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Isoprostanes

Isoprostanes are a class of prostaglandin-like compounds formed by the peroxidation of arachidonic acid, independent of cyclooxygenase.
They are considered reliable biomarkers of oxidative stress and have been implicated in various pathological conditions, including cardiovascular disease, neurodegenerative disorders, and cancer.
Isoprostanes exhibit potent biological activities and may play a role in the pathophysioogy of these diseases.
Research on isoprostanses is an active area of study, with ongoing efforts to develop sensitive detection methods and further elucidate their clinical utility.

Most cited protocols related to «Isoprostanes»

1
Biomarker Analysis of Urine and Blood
Urine samples were collected, and aliquots stored at −20 °C until batch analysis. Blood samples were separated immediately by centrifugation at 2000g for 15 minutes at 4 °C, and the aliquots were stored at −80 °C, within 30 minutes of blood collection, until batch analysis. High sensitivity C-reactive protein (hsCRP) was measured using Synchron systems CRPH reagent kit (Beckman-Coulter, UK) as per manufacturer’s protocol. Fasting plasma glucose (FPG) was measured using a Synchron LX 20 analyzer (Beckman-Coulter) according to the manufacturer’s recommended protocol. Total cholesterol, triglycerides, and high-density lipoprotein (HDL) cholesterol levels were measured enzymatically using a Synchron LX20 analyzer (Beckman-Coulter, High Wycombe, UK). Plasma metanephrine and normetanephrine were measured by tandem mass spectrometry. The between-run coefficients of variation (CV) for the metanephrine and normetanephrine measurements were 6.5–12.2% and 4.7–11.5%, respectively. Urinary isoprostane, 8-iso PGF2α, was measured by enzyme-linked immunosorbent assay (ELISA) using urinary isoprostane EIA kit (Oxford Biomedical Research, Oxford, USA) as per the manufacturer’s protocol, and by an operator who was blinded to study group of the participants24 (link).
Kahal H., Halama A., Aburima A., Bhagwat A.M., Butler A.E., Graumann J., Suhre K., Sathyapalan T, & Atkin S.L. (2020). Effect of induced hypoglycemia on inflammation and oxidative stress in type 2 diabetes and control subjects. Scientific Reports, 10, 4750.
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Publication 2020
8-isoprostaglandin F2alpha BLOOD Centrifugation Cholesterol C Reactive Protein Enzyme-Linked Immunosorbent Assay Glucose High Density Lipoprotein Cholesterol Isoprostanes Metanephrine Normetanephrine Plasma Tandem Mass Spectrometry Triglycerides Urine
2
Omega-3 Fatty Acids in ARDS: A Randomized Trial
With a maximum enrollment of 1000 patients and 4 planned interim analyses, the study had statistical power of 90.7% to detect a 2.25-day increase in VFDs, assuming a mean of 14 VFDs and standard deviation of 10.5. The study was monitored using a group sequential design with asymmetric stopping boundaries for efficacy and futility designed using alpha and beta spending boundaries (eMethods).19 An independent data and safety monitoring board (DSMB) conducted an analysis of serum n-3 levels after enrollment of the first 60 patients, a safety analysis after enrollment of 100 patients, and an interim analysis after enrollment of 272 patients. Although VFDs was the primary end point, the DSMB was advised to consider mortality in their decision to stop the trial for either efficacy or futility.
Means and standard deviations are reported for baseline continuous variables, and counts and percentages are reported for baseline categorical variables, with differences assessed using t tests and χ2 (link) tests, respectively. Plasma levels of IL-6, IL-8, leukotrienes, and urinary isoprostanes were log transformed and compared using analysis of variance with baseline levels as covariates. Categorical outcome variables are reported as percentages with 95% confidence intervals. The continuous outcome variables (VFDs, ICU-free days, and organ failure–free days) are reported as means and standard deviations, with differences assessed using analysis of variance controlling for baseline shock and enrollment group of the EDEN study.
Logistic regression controlling for baseline shock and randomization group of the EDEN study was used to analyze mortality. Adjusted mortality rates were calculated using 7 baseline mortality-predicting covariates derived from a previous study of similar populations20 (link): age, Acute Physiology and Chronic Health Evaluation III (APACHE III) score, plateau pressure, missing plateau pressure, number of organ failures, and the alveolar-arterial difference in PaO2 value. Proportion curves over time were plotted for survival and unassisted breathing.
All analyses were performed using SAS version 9.2 (SAS Institute Inc, Cary, North Carolina) on an intention-to-treat basis, with 2-sided P≤.05 considered significant. P values were not corrected for multiple comparisons or early stopping.
Rice T.W., Wheeler A.P., Thompson B.T., deBoisblanc B.P., Steingrub J, & Rock P. (2011). Enteral Omega-3 Fatty Acid, γ-Linolenic Acid, and Antioxidant Supplementation in Acute Lung Injury. JAMA : the journal of the American Medical Association, 306(14), 1574-1581.
Publication 2011
Arteries Clinical Trials Data Monitoring Committees Isoprostanes Leukotrienes Patients Patient Safety physiology Plasma Pressure Serum Shock Urine
3
Quantifying Alveolar and Interstitial Morphology
The point-counting technique was employed (26 (link)) by means of a reticule of a known area (50 lines and 100 points) coupled to a microscope (E200MV, Nikon Corporation, Tokyo, Japan). Ten random fields in the alveolar walls per animal, at a magnification of 1,000×, were counted, and the number of positive cells was established based on the number of points that coincided with the positive cells within the reticulum divided by the number of points coinciding with the alveolar walls. The total area of the reticulum was 104 μm2 and analyzes were performed at 1,000× magnification (17 (link), 27 (link)).
The optical density was used to evaluate the volume fractions of the collagen fibers type I and III, actin, decorin, lumican, biglycan, fibronectin, and isoprostane PGF2α. Images were captured using an Leica DM2500 microscope (Leica Microsystems, Wetzlar, Germany), a digital camera (Leica DFC420 Leica Microsystems, Wetzlar, Germany). The images were acquired and processed using Optimas v.4.10 software. We analyzed 10 fields per lamina and one lamina per animal. The images were analyzed using Image-Proplus 4.5 software (NIH, MD, USA). This software allowed a thresholding of the color shades to be developed. These shades represent the positive areas quantified in the previously determined area. The volume fractions of these markers are expressed as percentages of the area (20 (link)).
By using a light microscope (Leica DFC 420 Leica microsystems, Wetzlar, Germany), eight non-overlapping fields of view were imaged at 100×, 200×, and 400× magnifications. We used a weighted scoring system to quantify interstitial edema. Scores from 0 to 4 were used to represent the severity of interstitial edema, with 0 representing no effect and 4 representing maximal severity. Additionally, the degree of each score quantified per field of view was established on a scale of 0–4, with 0 representing no noticeable extent and 4 representing complete involvement. An evaluator blind to all animal treatment conditions performed all analyzes and product of severity and the extent of each feature, ranging from 0 to 16, was used to calculate the overall scores (28 (link), 29 (link)).
Camargo L.D., Righetti R.F., Aristóteles L.R., dos Santos T.M., de Souza F.C., Fukuzaki S., Cruz M.M., Alonso-Vale M.I., Saraiva-Romanholo B.M., Prado C.M., Martins M.D., Leick E.A, & Tibério I.D. (2018). Effects of Anti-IL-17 on Inflammation, Remodeling, and Oxidative Stress in an Experimental Model of Asthma Exacerbated by LPS. Frontiers in Immunology, 8, 1835.
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Publication 2017
Actins Animal Diseases Animals Biglycan Blindness Cells Collagen Type I Decorin Dinoprost Edema Fibronectins Fingers Isoprostanes Light Microscopy LUM protein, human Microscopy Reticulum Vision
4
Vascular Inflammation Biomarkers Analysis

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Publication 2013
8-epi-prostaglandin F2alpha Biological Assay Biological Markers BLOOD C Reactive Protein Enzyme-Linked Immunosorbent Assay Immunonephelometry Intercellular Adhesion Molecule-1 Interleukin-6 Isoprostanes Monocyte Chemoattractant Protein-1 Necrosis Neoplasms PAF 2-Acylhydrolase SELP protein, human TNFRSF1B protein, human Tumor Necrosis Factor Receptor 11b Urine Vasculitis
5
Meta-Analysis of F2-Isoprostanes as Oxidative Stress Biomarkers

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Publication 2017
8-epi-prostaglandin F2alpha 8-isoprostaglandin F2alpha Amyotrophic Lateral Sclerosis Biological Markers Biopharmaceuticals Coagulation, Blood Epilepsy F2-Isoprostanes Homo Hyperthyroidism Isoprostanes Oxidative Stress PTGS2 protein, human Serum

Most recents protocols related to «Isoprostanes»

1
Urinary Isoprostane Quantification Protocol
Urine samples from each rat were tested for 15-isoprostane F2t activity using a urinary isoprostane ELISA kit (Oxford Biomedical Research, Oxford, United States) according to the manufacturer’s instruction. The sample was prepared by adding 100 L of urine to an anti-15-isoprostane F2t-coated well plate following dilution with glucuronidase. The 15-isoprostane F2t horseradish peroxidase (HRP) conjugate and tetramethylbenzidine (TMB) substrate were then added to the mixture. An EnSpire Multimode Plate Reader (Perkin Elmer, Singapore) quantified the produced colour as the absorbance at 650 nm. Based on the data collected and the standard curve produced using the given standard solution, the isoprostane concentration (ng/mL) of each sample was calculated.
Razak A.M., Zakaria S.N., Abdul Sani N.F., Ab Rani N., Hakimi N.H., Mohd Said M., Tan J.K., Gan H.K., Mad Nordin M.F, & Makpol S. (2023). A subcritical water extract of soil grown Zingiber officinale Roscoe: Comparative analysis of antioxidant and anti-inflammatory effects and evaluation of bioactive metabolites. Frontiers in Pharmacology, 14, 1006265.
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Publication 2023
3,3',5,5'-tetramethylbenzidine 8-isoprostaglandin F2alpha beta-Glucuronidase Enzyme-Linked Immunosorbent Assay Horseradish Peroxidase Isoprostanes Technique, Dilution Urine
2
Urinary Biomarkers of Oxidative Stress
Urinary concentrations of 15-F2t-isoprostane, leukotriene B4 (LTB4), lipoxine A4 (LXA4), the metabolites of prostanglandine E2 metabolite (PGEM), reduced and oxidized glutathione (GSH and GSSG) and creatinine were measured using commercial enzyme immunoassays kits (Oxford Biomedical Research for 15-F2t-isoprostane, LTB4, LXA4 and PGEM, Cayman Chemical for GSH/GSSG, BioVision for creatinine) for CM patients at D0, D3 and D30, according to the manufacturer’s protocol. As recommended by the manufacturer, the samples were pre-treated with β-glucuronidase for 15-F2t-isoprostane dosage to separate isoprostane from glucuronic acid, with this complexed form representing more than 50% of the isoprostane present in the urine [27 (link)]. Similarly, prior to PGEM dosage, for reasons of PEG2 instability, standards and urine samples were subjected to derivatization to quantify a stable derivative. Finally, for the measurement of oxidized glutathione (GSSG), standards and samples were pretreated with 2-vinylpyridine to derivatize GSH. A second assay in the absence of pretreatment was carried out to determine the concentration of total GSH and the subsequent amount of reduced glutathione (GSH) equal to total GSH–GSSG. The plate reader TECAN Infinite F200 pro and dedicated software were used for plate readings. Values of the different urinary biomarkers were normalized to the urinary creatinine concentrations for each patient.
Royo J., Vianou B., Accrombessi M., Kinkpé E., Ayédadjou L., Dossou-Dagba I., Ladipo Y., Alao M.J., Bertin G.I., Cot M., Boumédiène F., Houzé S., Faucher J.F, & Aubouy A. (2023). Elevated plasma interleukin-8 as a risk factor for mortality in children presenting with cerebral malaria. Infectious Diseases of Poverty, 12, 8.
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Publication 2023
8-isoprostaglandin F2alpha beta-Glucuronidase Biological Assay Biological Markers Caimans Creatinine Enzyme Immunoassay Glucuronic Acid Glutathione Disulfide Isoprostanes Leukotriene B4 Patients Reduced Glutathione Urine
3
Comprehensive Evaluation of Antioxidant and Metabolic Effects
In the protocol the results of this study were originally planned to be published in three publications: 1. A hypothesis generating study on the effects on oxidative stress. Outcomes: SOD, CAT, GLU, isoprostanes and Hs CRP. 2. Confirmatory study on effects on serum-cholesterol levels with primary outcomes, TC, LDL-C, HDL-C, TG, secondary outcomes: Blood pressure, SOD, CAT, GLU, isoprostanes, HbA1c, DFI and Hs CRP. 3. Confirmatory study on effects on sperm quality with primary outcomes: TMSC, TPMSC. Secondary outcomes: Pregnancy during the study or within 3 months of terminating participation in the study, SOD, CAT, HbA1c, DFI and Hs CRP, testosterone, blood pressure. During evaluation of the results of the clinical study it was evaluated to be more sensible to publish all results in one paper presenting the primary and secondary outcomes listed above.
Sangild J., Faldborg A., Schousboe C., Fedder M.D., Christensen L.P., Lausdahl A.K., Arnspang E.C., Gregersen S., Jakobsen H.B., Knudsen U.B, & Fedder J. (2023). Effects of Chokeberries (Aronia spp.) on Cytoprotective and Cardiometabolic Markers and Semen Quality in 109 Mildly Hypercholesterolemic Danish Men: A Prospective, Double-Blinded, Randomized, Crossover Trial. Journal of Clinical Medicine, 12(1), 373.
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Publication 2023
Blood Pressure Cholesterol C Reactive Protein Isoprostanes Oxidative Stress Pregnancy Serum Sperm Testosterone
4
Investigating Oxidative Stress and Inflammation
The primary outcomes of blood samples were markers of OS and inflammation, serum lipid levels, long-term blood glucose (HbA1c), homeostatic model assessment (HOMA), and fasting glucose. The blood samples were analyzed in standardized hospital laboratories.
To investigate lipid status, TC, triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were analyzed.
C-reactive Protein (CRP) was the primary marker of inflammation. To analyze CRP-counts, the high sensitivity CRP (hs CRP) was used, as this test can detect slight changes within the normal ranges of CRP [52 (link)].
Conditions of OS were measured by levels of glutathione, SOD, CAT, and isoprostanes. Glutathione, SOD, and CAT are part of the antioxidant defense system scavenging ROS [30 (link),53 (link),54 (link)], and isoprostanes are regarded as valid indicators of cell membrane damage caused by OS [55 (link),56 (link)].
Sangild J., Faldborg A., Schousboe C., Fedder M.D., Christensen L.P., Lausdahl A.K., Arnspang E.C., Gregersen S., Jakobsen H.B., Knudsen U.B, & Fedder J. (2023). Effects of Chokeberries (Aronia spp.) on Cytoprotective and Cardiometabolic Markers and Semen Quality in 109 Mildly Hypercholesterolemic Danish Men: A Prospective, Double-Blinded, Randomized, Crossover Trial. Journal of Clinical Medicine, 12(1), 373.
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Publication 2023
Antioxidants BLOOD Blood Glucose Cholesterol, beta-Lipoprotein C Reactive Protein Glucose Glutathione High Density Lipoprotein Cholesterol Homeostasis Hypersensitivity Inflammation Isoprostanes Lipids Plasma Membrane Serum Triglycerides
5
Quantification of Bioactive Lipid Mediators
The levels of free F2-IsoPs, F3-IsoPs, and F4-NeuroPs were determined by gas chromatography/negative-ion chemical ionization tandem mass spectrometry (GC/NICI-MS/MS; Thermo Finnigan, San Jose, CA, USA).
After thawing, the seminal and blood plasma samples were treated with a volume of acidified water (pH 3) and spiked with a tetradeuterated derivative of Prostaglandin F2α (PGF2α-d4; 500 pg), as an internal standard. Subsequently, solid phase extraction procedures were carried out, according to a previously reported methodology [32 (link)]. Briefly, each sample (plasma or seminal plasma) was applied to an octadecylsilane (C18) cartridge, and the eluate was transferred to an aminopropyl (NH2) cartridge to collect the isoprostanoids. The final eluates were derivatized to convert the carboxyl group of isoprostanes into pentafluorobenzyl esters and the hydroxyl group into trimethylsilyl ethers, as previously reported [33 (link)]. The derivatized isoprostanoids were analyzed by GC/NICI-MS/MS. The ions that were determined were the product ions at m/z 299 and m/z 303, derived from the [M-181]—precursor ions of 8-iso-PGF2α, also referred to as 15-F2t-IsoP (m/z 569) and PGF2α-d4 (m/z 573), respectively [33 (link)], whereas for F3-IsoP quantification, the measured ion was the product ion at m/z 297 derived from the [M-181]− precursor ions (m/z 567) produced from oxidized EPA.
Concerning F4-NeuroPs, the mass ions that were determined were the product ions at m/z 323 and m/z 303, derived from the [M-181]-precursor ions of 10-F4tNeuroPs, considered as the most represented F4-NeuroPs (m/z 593) [34 (link)] and PGF2α-d4 (m/z 573), respectively.
Mattioli S., Angelucci E., Dal Bosco A., Signorini C., Sylla L., Bosa L., Collodel G., Durand T., Galano J.M., Oger C, & Castellini C. (2023). Pro-Atherogenic and Pro-Oxidant Diets Influence Semen and Blood Traits of Rabbit Bucks. Antioxidants, 12(10), 1880.
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Publication 2023
8-isoprostaglandin F2alpha Dinoprost Esters Ethers Gas Chromatography-Mass Spectrometry Geodia Hydroxyl Radical Ions Isoprostanes octadecylsilane Plasma Seminal Plasma Solid Phase Extraction Tandem Mass Spectrometry

Top products related to «Isoprostanes»

1
Urinary isoprostane elisa kit by Oxford Biomedical Research
Sourced in United States
The Urinary Isoprostane ELISA Kit is a quantitative assay designed to measure the levels of isoprostanes, a biomarker of oxidative stress, in urine samples. The kit uses a competitive enzyme-linked immunosorbent assay (ELISA) principle to determine the concentration of isoprostanes in the provided samples.
2
8 isoprostane eia kit by Cayman Chemical
Sourced in United States
The 8-isoprostane EIA kit is a laboratory assay designed to quantify the levels of 8-isoprostane, a biomarker of oxidative stress, in various sample types. The kit utilizes a competitive enzyme immunoassay (EIA) technique to measure 8-isoprostane concentrations.
3
Elisa kit by Oxford Biomedical Research
Sourced in United States
The ELISA kit is a laboratory tool used to detect and quantify specific proteins or other molecules in a sample. It employs an enzyme-linked immunosorbent assay (ELISA) technique to measure the concentration of the target analyte. The kit includes the necessary reagents and materials required to perform the assay.
4
Urinary isoprostane eia kit by Oxford Biomedical Research
Sourced in United States
The Urinary Isoprostane EIA Kit is a tool designed to quantify the levels of isoprostanes, a class of prostaglandin-like compounds, in urine samples. Isoprostanes are considered reliable biomarkers of oxidative stress, which is associated with various pathological conditions. This kit provides a standardized and convenient method to measure urinary isoprostane levels.
5
Elisa kit by Cayman Chemical
Sourced in United States, China
The ELISA (Enzyme-Linked Immunosorbent Assay) kit is a laboratory tool used for the detection and quantification of specific substances, such as proteins, hormones, or antibodies, in a sample. The kit contains the necessary reagents and components to perform the ELISA technique, which is a widely used analytical method in various fields, including research, diagnostics, and quality control.
6
Eia kit by Cayman Chemical
Sourced in United States
The EIA kit is a laboratory tool used to detect and quantify specific molecules in a sample through enzyme-linked immunosorbent assay (ELISA) technique. It provides a standardized and reliable method for analyte measurement.
7
Formic acid by Merck Group
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Formic acid is a colorless, pungent-smelling liquid chemical compound. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid is widely used in various industrial and laboratory applications.
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Tgf β1 by R&D Systems
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TGF-β1 is a cytokine that plays a key role in cell growth, cell differentiation, and immune function. It is part of the transforming growth factor beta family of proteins. TGF-β1 is commonly used in cell culture research.
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Strata x aw 33u polymeric by Phenomenex
Strata X-AW 33u polymeric is a solid-phase extraction (SPE) sorbent. It is a polymeric material designed for the extraction and purification of analytes from various matrices.
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Luminex is a multiplex assay platform that allows for the simultaneous detection and quantification of multiple analytes in a single sample. The core function of Luminex is to facilitate high-throughput, multiplexed analysis of proteins, nucleic acids, and other biomolecules.

More about "Isoprostanes"

Isoprostanes are a fascinating class of compounds that have captured the attention of researchers worldwide.
These prostaglandin-like molecules are formed through the peroxidation of arachidonic acid, independent of the cyclooxygenase pathway.
As reliable biomarkers of oxidative stress, isoprostanes have been implicated in a wide range of pathological conditions, including cardiovascular disease, neurodegenerative disorders, and cancer.
Isoprostanes exhibit potent biological activities and are believed to play a crucial role in the pathophysiology of these diseases.
Researchers are actively exploring the clinical utility of isoprostanes, with ongoing efforts to develop sensitive detection methods, such as ELISA kits (e.g., Urinary Isoprostane ELISA Kit, 8-isoprostane EIA kit) and Luminex-based assays.
The study of isoprostanes is an exciting and rapidly evolving field, with researchers leveraging advanced tools and technologies to uncover their secrets.
The Formic acid extraction method and the use of Strata X-AW 33u polymeric sorbents have become important steps in the accurate quantification of isoprostanes, particularly in the analysis of urinary samples (Urinary isoprostane EIA kit).
Moreover, the transforming growth factor-beta 1 (TGF-β1) pathway has been implicated in the regulation of isoprostane production, adding another layer of complexity to the understanding of their role in disease processes.
PubCompare.ai, the leading AI platform for isoprostane research, offers a wealth of resources and insights to support scientists in their quest to push the boundaries of knowledge.
By seamlessly locating protocols from literature, preprints, and patents, and leveraging AI-driven comparisons to identify the optimal methods and products, PubCompare.ai empowers researchers to take their isoprostane studies to new heights and accelerate their discoveries.