The broth microdilution method was used to measure the minimal inhibitory concentration (MIC) of oridonin against USA300 as per the guidelines of the Clinical and Laboratory Standards Institute (Arendrup et al. 2017 ). The lowest concentration of oridonin that inhibited the growth of USA300 was defined as the MIC. The minimum bactericidal concentration (MBC) value against USA300 was determined with the BHI plate method (Wilson et al. 2018 (link)). Control experiment was carried out using a fixed concentration of DMSO in duplicates. To examine growth curves of USA300 following exposure to oridonin, 5 mL overnight bacterial cultures were added to 500 mL fresh BHI broth. After the culture absorbance at 600 nm (OD600 nm) reached 0.3, the bacterial cultures were treated with oridonin at final concentrations of 0, 8, 16, 32, 64 and 128 μg/mL. The cultures were incubated under constant shaking (200 rpm) at 37 °C (Ouyang et al. 2017 (link)). The OD600 nm value of the culture was measured at regular intervals with a UV-spectrophotometer (UV-2000, UNICO, Shanghai, China), and the growth curve was plotted according to the absorbance value. The experiments were repeated three times.
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Biologically Active Substance
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Oridonin
Oridonin
Oridonin: A Potent Triterpenoid Compound with Diverse Pharmacological Activities.
Oridonin is a bioactive compound isolated from various plant species, primarily within the genus Isodon.
It has been extensively studied for its anti-cancer, anti-inflammatory, and neuroprotective properties.
Oridonin exhibits a wide range of biological effects, including the regulation of cell proliferation, apoptosis, and autophagy.
Researchers continue to explore the therapeutic potential of Oridonin in the treatment of cancer, neurological disorders, and other disease states.
This MeSH term provides a concise overview of the key characteristics and applications of this important natural product.
Oridonin is a bioactive compound isolated from various plant species, primarily within the genus Isodon.
It has been extensively studied for its anti-cancer, anti-inflammatory, and neuroprotective properties.
Oridonin exhibits a wide range of biological effects, including the regulation of cell proliferation, apoptosis, and autophagy.
Researchers continue to explore the therapeutic potential of Oridonin in the treatment of cancer, neurological disorders, and other disease states.
This MeSH term provides a concise overview of the key characteristics and applications of this important natural product.
Most cited protocols related to «Oridonin»
Bacteria
Clinical Laboratory Services
Minimum Inhibitory Concentration
oridonin
Sulfoxide, Dimethyl
Buffers
High-Performance Liquid Chromatographies
oridonin
Sodium Chloride
Actins
Aftercare
BCL2 protein, human
Caspase 3
Cell Extracts
Cells
Chemiluminescence
Electrophoresis
Immunoglobulins
Malignant Neoplasm of Breast
MDA-MB-231 Cells
Milk
oridonin
PARP1 protein, human
polyacrylamide gels
polyvinylidene fluoride
Proteins
RELA protein, human
Serum Albumin, Bovine
Sulfoxide, Dimethyl
Technique, Dilution
Tissue, Membrane
Cell Nucleus
Cells
Endoribonucleases
Flow Cytometry
Fluorescence
Phosphates
polyethylene glycol 8000
Propidium Iodide
Saline Solution
Sodium Chloride
Sodium Citrate
Triton X-100
In all, 6–8-weeks-old C57BL/6 mice were used to induce peritonitis by intraperitoneal administration of 1 mg MSU crystals (dissolved in 0.5 ml PBS). Before injection of MSU, 20 mg/kg Ori (dissolved in vehicle containing 90% PBS and 10% DMSO) were injected intraperitoneally. After 6 h, the mice were killed and 10 ml ice-cold PBS were used to wash the peritoneal cavities. The polymorphonuclear neutrophils in peritoneal lavage fluid was analyzed by flow cytometry by staining Ly6G and CD11b. The IL-1β level in serum or peritoneal lavage fluid was determined by ELISA.
For inducing joint inflammation, mice were administered Ori (20 mg/kg, dissolved in DMSO) by intraarticular injection. After 30 min, 0.5 mg MSU (dissolved in 20 μL PBS) was administrated intraarticularly and then the size of joints was measured at different time points. After 24 h, the patella were isolated and cultured in 200 μl opti-MEM medium containing 1% Penicillin-Streptomycin at room temperature for 1 h.
For inducing joint inflammation, mice were administered Ori (20 mg/kg, dissolved in DMSO) by intraarticular injection. After 30 min, 0.5 mg MSU (dissolved in 20 μL PBS) was administrated intraarticularly and then the size of joints was measured at different time points. After 24 h, the patella were isolated and cultured in 200 μl opti-MEM medium containing 1% Penicillin-Streptomycin at room temperature for 1 h.
Arthritis
Cold Temperature
Culture Media
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Injections, Intraperitoneal
Interleukin-1 beta
Intra-Articular Injections
ITGAM protein, human
Joints
Mice, Inbred C57BL
Mus
Neutrophil
Patella
Penicillins
Peritoneal Cavity
Peritoneal Fluid
Peritoneal Lavage
Peritonitis
Serum
Streptomycin
Sulfoxide, Dimethyl
Most recents protocols related to «Oridonin»
Soybean lecithin, DSPE-PEG2000 or DSPE-PEG2000-Maleimide, and oridonin were mixed at a mass ratio of 10:2:1. This mixture was then dissolved in dichloromethane (DCM). After fully evaporating, distillated ddH2O was added subsequently, and sonicated in the water bath for 10 ~ 20 min, resulting in a semi-transparent, white suspension. The final products were designated as orid-liposome and orid-liposome-MAL, respectively. Subsequently, orid-liposome-MAL were divided equally into two parts. One part was used for subsequent cell experiments, and the other for preparing targeted orid-liposome.
A cysteine (Cys) residue was synthetically appended to the C-terminal of the TLR2 targeting peptide, resulting in an amino acid sequence represented as His-Leu-Tyr-Val-Ser-Pro-Trp-Cys. TLR2 targeting peptide was co-incubated and conjugated to the orid-liposome-MAL. The drugs named as 1:1 and 5:1 differed only in the amount of TLR2 pep. TLR2 pep-orid-liposome 1:1 represents the molar ratio of 1:1 for oridonin: TLR2 peptide, whereas 5:1 for TLR2 pep-orid-liposome 5:1. Dialysis was performed to eliminate any excess free peptide post-incubation.
The MIC of oridonin was determined via the broth dilution method, as described by Paudel et al. (2019) [24 (link)]. Oridonin was sterilized via filtration using a 0.22 μm millipore membrane filter attached to a syringe (Millipore, Nanjing, China). Two-fold dilutions of sterile oridonin ranging from 800 to 6.25 μg/mL were aseptically prepared in tubes of sterile NB. The tubes of NB were each inoculated with 50 μL of overnight (20 h) cultures of A. hydrophila AS 1.1801 to obtain an initial viable cell concentration of 5.0 Log CFU/mL. The experiment included positive (inoculated tubes, free of oridonin) and negative (non-inoculated tubes, free of oridonin) controls. All tubes were incubated at 28 °C and observed for turbidity after 24 h. The MIC was the lowest oridonin concentration at which no visible growth (absence of turbidity) occurred. Tests for MIC were performed in triplicates.
The effect of subinhibitory concentrations of oridonin on A. hydrophila AS 1.1801 was determined spectrophotometrically using microplate readers (Infinite F50, TECAN, Männedorf, Switzerland). Concisely, overnight cultures of A. hydrophila AS 1.1801 were inoculated into NB broth without or with oridonin at different concentrations (0, 3.125, 6.25, 12.5, 25, 50, 100, and 200 μg/mL) at 28 °C for 26 h (stationary phase) under the condition of shaking at 180 rpm. At each of the time points viz. 0, 2, 4, 6, 8, 10, 12, 24, and 26 h, 200 μL of the oridonin-treated or untreated inoculum was added to a 96-well plate and determined for optical density (OD) at 600 nm. The experiments were repeated three times.
Nucleic acids (DNA and RNA) released from the cells of A. hydrophila AS 1.1801 after treatment with oridonin at different concentrations (0, 1/4, 1/2, 1, and 2 × MIC) were analyzed according to the method described by Kang and Song (2019) [26 (link)], with the introduction of some modifications. Overnight cultures grown on an NB were used for making a bacterial suspension in physiological saline (0.85% NaCl) and further adjusted to 0.5 McFarland turbidity using a McFarland densitometer (DEN-1, BIOSAN, Riga, Latvia). Inoculum were collected via centrifugation (8000 rpm, 4 °C for 10 min), washed with physiological saline, mixed with 20 mL physiological saline containing different concentrations of oridonin, and cultured at 28 °C for 3 h, 6 h, and 9 h under shaking conditions at 180 rpm. After incubation, the cell pellets treated with or without oridonin were harvested via centrifugation, as described above. The supernatant was collected, and its absorbance at 260 nm was measured to determine the amounts of intracellular DNA and RNA released from the cells of A. hydrophila AS 1.1801 after oridonin treatment. The supernatant obtained from the untreated A. hydrophila AS 1.1801 inoculum was used as a control.
Top products related to «Oridonin»
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
Sourced in United States
Oridonin is a natural compound extracted from the Chinese herbal plant Isodon. It is a white crystalline powder that is commonly used in laboratory research applications. Oridonin exhibits various biological activities and is often employed as a research tool in the study of cellular processes and signaling pathways.
Sourced in China
Oridonin is a natural bioactive compound extracted from the traditional Chinese medicinal plant Isodon rubescens. It is a white crystalline powder that serves as a key raw material for pharmaceutical and research applications.
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
Sourced in United States, United Kingdom, China, Germany, Japan, Canada, Morocco, Sweden, Netherlands, Switzerland, Italy, Belgium, Australia, France, India, Ireland
β-actin is a cytoskeletal protein that is ubiquitously expressed in eukaryotic cells. It is an important component of the microfilament system and is involved in various cellular processes such as cell motility, structure, and integrity.
Sourced in United States, Germany, China, United Kingdom, Morocco, Ireland, France, Italy, Japan, Canada, Spain, Switzerland, New Zealand, India, Hong Kong, Sao Tome and Principe, Sweden, Netherlands, Australia, Belgium, Austria
PVDF membranes are a type of laboratory equipment used for a variety of applications. They are made from polyvinylidene fluoride (PVDF), a durable and chemically resistant material. PVDF membranes are known for their high mechanical strength, thermal stability, and resistance to a wide range of chemicals. They are commonly used in various filtration, separation, and analysis processes in scientific and research settings.
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RPMI 1640 medium is a commonly used cell culture medium developed at Roswell Park Memorial Institute. It is a balanced salt solution that provides essential nutrients, vitamins, and amino acids to support the growth and maintenance of a variety of cell types in vitro.
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Matrigel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins. It is widely used as a substrate for the in vitro cultivation of cells, particularly those that require a more physiologically relevant microenvironment for growth and differentiation.
More about "Oridonin"
Oridonin is a bioactive triterpenoid compound isolated from various plant species, primarily from the Isodon genus.
This natural product has been extensively studied for its remarkable pharmacological activities, including anti-cancer, anti-inflammatory, and neuroprotective properties.
Oridonin's mechanism of action involves the regulation of key cellular processes such as proliferation, apoptosis, and autophagy.
Researchers have explored the therapeutic potential of Oridonin in the treatment of a wide range of disease states, including cancer, neurological disorders, and inflammatory conditions.
In cancer research, Oridonin has shown promising results in inhibiting the growth and proliferation of various cancer cell lines, while also inducing apoptosis and autophagy.
To facilitate Oridonin research, researchers often utilize common laboratory reagents and techniques, such as FBS (Fetal Bovine Serum) for cell culture, TRIzol reagent for RNA extraction, DMSO (Dimethyl Sulfoxide) as a solvent, and DMEM (Dulbecco's Modified Eagle Medium) or RPMI 1640 medium for cell culture.
Western blotting techniques, employing PVDF (Polyvinylidene Fluoride) membranes and β-actin as a loading control, are frequently used to analyze protein expression in Oridonin-treated cells.
Additionally, advanced in vitro assays, such as the Matrigel invasion assay, have been utilized to evaluate the anti-metastatic potential of Oridonin.
By leveraging the insights gained from this comprehensive understanding of Oridonin's pharmacological properties and research methodologies, scientists can more effectively navigate the complexities of Oridonin research and optimize their investigations for greater reproducibility and productivity.
This natural product has been extensively studied for its remarkable pharmacological activities, including anti-cancer, anti-inflammatory, and neuroprotective properties.
Oridonin's mechanism of action involves the regulation of key cellular processes such as proliferation, apoptosis, and autophagy.
Researchers have explored the therapeutic potential of Oridonin in the treatment of a wide range of disease states, including cancer, neurological disorders, and inflammatory conditions.
In cancer research, Oridonin has shown promising results in inhibiting the growth and proliferation of various cancer cell lines, while also inducing apoptosis and autophagy.
To facilitate Oridonin research, researchers often utilize common laboratory reagents and techniques, such as FBS (Fetal Bovine Serum) for cell culture, TRIzol reagent for RNA extraction, DMSO (Dimethyl Sulfoxide) as a solvent, and DMEM (Dulbecco's Modified Eagle Medium) or RPMI 1640 medium for cell culture.
Western blotting techniques, employing PVDF (Polyvinylidene Fluoride) membranes and β-actin as a loading control, are frequently used to analyze protein expression in Oridonin-treated cells.
Additionally, advanced in vitro assays, such as the Matrigel invasion assay, have been utilized to evaluate the anti-metastatic potential of Oridonin.
By leveraging the insights gained from this comprehensive understanding of Oridonin's pharmacological properties and research methodologies, scientists can more effectively navigate the complexities of Oridonin research and optimize their investigations for greater reproducibility and productivity.