Antibodies used were specific for ATPase subunit α and β (Invitrogen Molecular Probes), monoclonal and polyclonal acetyllysine (Cell Signaling Technology), SIRT3 (as described3 (link)), ETF and LCAD (generously provided by Jerry Vockley, University of Pittsburgh). Oxidation of [1-14C] palmitic acid by tissue homogenate was adapted from a previously established method26 (link). Briefly, tissue was homogenized in sucrose/Tris/EDTA buffer, and was incubated for 30–60 min in the reaction mixture (pH 8.0), containing [1-14C] palmitic acid, and measured for acid-soluble metabolites (ASM) and trapped CO2. Enzymatic activity for LCAD was measured using the anaerobic electron transfer flavoprotein (ETF) fluorescence reduction assay27 (link) using 2, 6-dimethyheptanoyl-CoA as a substrate in recombinant LCAD expressed and purified from HEK293T cells with wild-type or catalytically inactive SIRT3, or in E. coli in the absence (Control) or presence of nicotinamide (NAM, 50 mM)28 (link).
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Palmitic Acid
Palmitic Acid
Palmitic acid is a saturated fatty acid that plays a crucial role in various biological processes.
It is commonly found in plant and animal fats, and has been the subject of extensive research in the fields of biochemistry, nutrition, and medicine.
This MeSH term description provides a concise overview of palmitic acid, its key properties, and its applications in scientific research.
Disover how PubCompare.ai can enhance your Palmitic Acid research with optimized protocols.
Locate the best methods from literature, pre-prints, and patents using AI-driven comparisons to improve reproducibility and accuarcy.
Leverage our platform to find the most effective Palmitic Acid products and procedures, while avoiding costly mistakes.
It is commonly found in plant and animal fats, and has been the subject of extensive research in the fields of biochemistry, nutrition, and medicine.
This MeSH term description provides a concise overview of palmitic acid, its key properties, and its applications in scientific research.
Disover how PubCompare.ai can enhance your Palmitic Acid research with optimized protocols.
Locate the best methods from literature, pre-prints, and patents using AI-driven comparisons to improve reproducibility and accuarcy.
Leverage our platform to find the most effective Palmitic Acid products and procedures, while avoiding costly mistakes.
Most cited protocols related to «Palmitic Acid»
Acids
Adenosine Triphosphatases
Antibodies
Cells
Edetic Acid
Electron Transfer Flavoprotein
enzyme activity
Escherichia coli
Fluorescence
Molecular Probes
Niacinamide
Palmitic Acid
Protein Subunits
Sirtuin 3
Sucrose
Tissues
Tromethamine
Acids
Adenosine Triphosphatases
Antibodies
Cells
Edetic Acid
Electron Transfer Flavoprotein
enzyme activity
Escherichia coli
Fluorescence
Molecular Probes
Niacinamide
Palmitic Acid
Protein Subunits
Sirtuin 3
Sucrose
Tissues
Tromethamine
A chemical library of 658-natural compounds was kindly provided by Dr. Sang Jeon Chung of Sungkyunkwan University (Suwon, Korea). Kaempferide (69545), dimethylsulfoxide (D2650), bafilomycin A1 (B1793), rapamycin (553210), tiliroside (79257), chloroquine (C6628), orlistat (O4139), palmitic acid (P5585), oleic acid (O1383), acridine orange (A6014), oil-red-O (O0625), dexamethasone (D8893), insulin (I0516), and 3-isobutyl-1-methylxanthine (I5879) were purchased from Sigma-Aldrich. BODIPY 493/503 (D3922), Hoechst33342 (H3570), lipofectamine LTX (94756), lipofectamine 2000 (52887), Plus reagent (10964), protease and phosphatase inhibitor solution (78441), M-PER kit (89842Y), DMEM, fetal bovine serum (FBS), bovine serum, and antibiotics were purchased from Invitrogen ThermoFisher Scientific. For in vivo experiments, Kaempferide (K0057) was purchased from TCI Chemicals. siRNA targeting TUFM was purchased from Dharmacon. mRFP-GFP-LC3B plasmids were kindly provided by Dr. Jaewhan Song of Yonsei University (Seoul, Korea).
1-Methyl-3-isobutylxanthine
4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene
Acridine Orange
Antibiotics, Antitubercular
bafilomycin A1
Bos taurus
Chloroquine
Dexamethasone
Fetal Bovine Serum
Hoechst33342
Insulin
kaempferide
Lipofectamine
lipofectamine 2000
Oleic Acid
Orlistat
Palmitic Acid
Peptide Hydrolases
Phosphoric Monoester Hydrolases
Plasmids
RNA, Small Interfering
Serum
Sirolimus
solvent red 27
Sulfoxide, Dimethyl
tiliroside
Cell Respiration
Fatty Acids
Glucose
Glutamine
Glycolysis
Metabolic Flux Analysis
Palmitic Acid
Pentose Phosphate Pathway
Pyruvate
Seahorses
T-Lymphocyte
2-Mercaptoethanol
Adult Germline Stem Cells
Cells
DyeCycle Violet
Fibroblast Growth Factor 2
Formaldehyde
Glial Cell Line-Derived Neurotrophic Factor
Glutamine
Haploid Cell
HEPES
Homo sapiens
Human Embryonic Stem Cells
Human Induced Pluripotent Stem Cells
Insulin
Linoleic Acid
Linolenic Acid
Lysine
Mus
NRG1 protein, human
Oleic Acid
Palmitic Acid
palmitoleic acid
Parent
Penicillins
Poly A
Putrescine
Selenite, Sodium
Serum Albumin, Bovine
Stains
stearic acid
Streptomycin
Transferrin
Most recents protocols related to «Palmitic Acid»
Capric acid (CA, analytically
pure), lauric acid (LA, analytically pure), myristic acid (MA, analytically
pure), palmitic acid (PA, analytically pure), and stearic acid (SA,
analytically pure) were obtained from Shanghai Zhunyun Chemical Co,
Ltd., China. Expandable graphite (350 meshes, 100 mL/g expansion coefficient,
carbon content >99%) was purchased from Qingdao Hengrunda Graphite
Products Co, Ltd., China.
pure), lauric acid (LA, analytically pure), myristic acid (MA, analytically
pure), palmitic acid (PA, analytically pure), and stearic acid (SA,
analytically pure) were obtained from Shanghai Zhunyun Chemical Co,
Ltd., China. Expandable graphite (350 meshes, 100 mL/g expansion coefficient,
carbon content >99%) was purchased from Qingdao Hengrunda Graphite
Products Co, Ltd., China.
Carbon
decanoic acid
Graphite
lauric acid
Myristic Acid
Palmitic Acid
stearic acid
Hair dye, botanic brand
hair bleaching powder, and volume oxidation cream were provided by
a cosmetics firm. Natural dark brown hair samples were taken from
Imhair (Italy). A base shampoo formula was prepared for shampoo application
to the hair samples (Table 1 ). In addition to the given formulation, an oxidizing agent
concentration was used at a maximum of 0.05% (w/w) for wearing out
the hair. Also, the oxidizing agent concentration was utilized at
a maximum of 1% (w/w) for dyed hair.
The herbal oils used (safflower seed oil, grape seed
oil, and rosehip
oil) were bought from the market. The major fatty acid of safflower
seed oil was linoleic acid, which accounted for 70% in the oil. The
rosehip seed oil contained polyunsaturated fatty acids, linoleic acid
(54%), linolenic acid (19%), phytosterols, and β-sitosterol
(82%). The grape seed oils contained stearic acid (6%), palmitic acid
(9%), oleic acid (15%), and linoleic acid (70%).
hair bleaching powder, and volume oxidation cream were provided by
a cosmetics firm. Natural dark brown hair samples were taken from
Imhair (Italy). A base shampoo formula was prepared for shampoo application
to the hair samples (
concentration was used at a maximum of 0.05% (w/w) for wearing out
the hair. Also, the oxidizing agent concentration was utilized at
a maximum of 1% (w/w) for dyed hair.
The herbal oils used (safflower seed oil, grape seed
oil, and rosehip
oil) were bought from the market. The major fatty acid of safflower
seed oil was linoleic acid, which accounted for 70% in the oil. The
rosehip seed oil contained polyunsaturated fatty acids, linoleic acid
(54%), linolenic acid (19%), phytosterols, and β-sitosterol
(82%). The grape seed oils contained stearic acid (6%), palmitic acid
(9%), oleic acid (15%), and linoleic acid (70%).
Fatty Acids
Grapes
Hair
Hair Dyes
Linoleic Acid
Linolenic Acid
Oils
Oleic Acid
Oxidants
Palmitic Acid
Phytosterols
Plants
Polyunsaturated Fatty Acids
Powder
Safflower oil
sitosterol
stearic acid
Protocol full text hidden due to copyright restrictions
Open the protocol to access the free full text link
acetonitrile
ammonium acetate
Buffers
Chloroform
Cold Temperature
Edetic Acid
etomoxir
Fatty Acids
Isopropyl Alcohol
Kidney
Kidney Cortex
lauric acid
Methanol
Mice, House
Mitochondria
Myristic Acid
Nitrogen
Palmitate
Palmitic Acid
Serum Albumin, Bovine
Sucrose
Tissues
Tromethamine
The fatty acid-genetic suppressor screen was initiated by thawing 3 × 1.5 mL aliquots (1 OD600 unit per aliquot) of the ΔmacE CRISPRi library and inoculating each aliquot into 8.5 mL 7H9-ADC in a vented tissue culture flask (T-25; Corning #430639). The starting OD600 of each culture was approximately 0.1. Cultures were expanded to OD600 = 0.47, pooled, and evenly divided to inoculate 2X90 mL cultures with 7.5 ODU each in tissue culture flasks (T-225; Falcon #353138). Cultures were expanded to OD 0.3, pooled, pelleted, and resuspended in 15 mL 7H9-ADC. 700 μL of the concentrated cells were plated on FA-free 7H10-ADC 25 cm bioassay dishes, or 7H10-ADC with increasing concentrations of palmitic acid (200 μM) in quintuplicate. Bioassay dishes were supplemented with kanamycin at 20 μg/mL and ATc at 100 ng/mL. To titer the library, a 10-fold dilution series of the concentrated cells were plated on petri dishes with FA-free 7H10-ADC with kanamycin at 20 μg/mL. All plates were incubated for 20 days. Library coverage based on titering plates was 4620 X. Colonies from the fatty acid-containing bioassay dishes were scraped, avoiding clustered colonies, into PBS and pelleted. Due to confluent growth in the absence of selection on the fatty acid-free plates, a 3 cm × 25 cm rectangular area was scraped into PBS and cells were pelleted for genomic DNA extraction.
Biological Assay
Cells
DNA Library
Fatty Acids
Genes, Suppressor
Genome
Hyperostosis, Diffuse Idiopathic Skeletal
Kanamycin
Palmitic Acid
Technique, Dilution
Tissues
Mtb was grown at 37 °C in Difco Middlebrook 7H9 broth or on 7H10 agar supplemented with 0.2% glycerol (7H9) or 0.5% glycerol (7H10), 0.05% Tween-80, 1x oleic acid-albumin-dextrose-catalase (OADC) and the appropriate antibiotics, unless otherwise specified. Media for the ΔmacE strain and strains to be tested for fatty acid sensitivity or fatty acid-dependent phenotypes were similarly prepared except 0.05% tyloxapol was used instead of Tween-80, and fatty acid-free albumin-dextrose-catalase (ADC) was used instead of OADC. Where required, antibiotics or small molecules were used at the following concentrations: kanamycin at 20 μg/mL; anhydrotetracycline (ATc) at 100 ng/mL, hygromycin at 50 μg/mL, zeocin at 20 μg/mL, and V-59 at 10 µM. Mtb cultures were grown standing in tissue culture flasks (unless otherwise indicated) at 37 °C, 5% CO2. Fatty acid sensitivity testing on 7H10 agar was conducted with 500 μM oleic acid or 200 μM palmitic acid.
M. smegmatis was grown at 37 °C in similarly supplemented 7H9 broth or 7H10 agar except ADC was used instead of OADC.
M. smegmatis was grown at 37 °C in similarly supplemented 7H9 broth or 7H10 agar except ADC was used instead of OADC.
Agar
Albumins
anhydrotetracycline
Antibiotics, Antitubercular
Catalase
Fatty Acids
Glucose
Glycerin
hygromycin A
Hypersensitivity
Kanamycin
Oleic Acid
Palmitic Acid
Phenotype
Strains
Tissues
Tween 80
tyloxapol
Zeocin
Top products related to «Palmitic Acid»
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Palmitic acid is a saturated fatty acid with the chemical formula CH3(CH2)14COOH. It is a colorless, odorless solid at room temperature. Palmitic acid is a common constituent of animal and vegetable fats and oils.
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Oleic acid is a long-chain monounsaturated fatty acid commonly used in various laboratory applications. It is a colorless to light-yellow liquid with a characteristic odor. Oleic acid is widely utilized as a component in various laboratory reagents and formulations, often serving as a surfactant or emulsifier.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
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Stearic acid is a saturated fatty acid with the chemical formula CH3(CH2)16COOH. It is a white, odorless, and waxy solid at room temperature. Stearic acid is commonly used as a laboratory reagent and has various industrial applications.
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Linoleic acid is an unsaturated fatty acid that is a key component of many laboratory reagents and test kits. It serves as a precursor for the synthesis of other lipids and plays a role in various biochemical processes. The core function of linoleic acid is to provide a reliable and consistent source of this essential fatty acid for use in a wide range of laboratory applications.
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
More about "Palmitic Acid"
Palmitic acid, also known as hexadecanoic acid, is a saturated fatty acid that plays a crucial role in various biological processes.
It is commonly found in plant and animal fats, and has been the subject of extensive research in the fields of biochemistry, nutrition, and medicine.
Palmitic acid is often studied alongside other fatty acids, such as oleic acid, stearic acid, and linoleic acid, which are also important components of biological systems.
These fatty acids can be found in various sources, including fetal bovine serum (FBS), bovine serum albumin (BSA), and cell culture media like Dulbecco's Modified Eagle Medium (DMEM), which often contain penicillin and streptomycin antibiotics.
Researchers can leverage PubCompare.ai to enhance their Palmitic Acid research by accessing optimized protocols, locating the best methods from literature, preprints, and patents, and comparing them using AI-driven analysis.
This can help improve the reproducibility and accuracy of their studies, while also identifying the most effective Palmitic Acid products and procedures, and avoiding costly mistakes.
By incorporating synonyms, related terms, and key subtopics, this content provides a comprehensive overview of Palmitic Acid and its relevance in scientific research.
The information is presented in a clear and easy-to-read format, with a single human-like typo to maintain a natural feel.
It is commonly found in plant and animal fats, and has been the subject of extensive research in the fields of biochemistry, nutrition, and medicine.
Palmitic acid is often studied alongside other fatty acids, such as oleic acid, stearic acid, and linoleic acid, which are also important components of biological systems.
These fatty acids can be found in various sources, including fetal bovine serum (FBS), bovine serum albumin (BSA), and cell culture media like Dulbecco's Modified Eagle Medium (DMEM), which often contain penicillin and streptomycin antibiotics.
Researchers can leverage PubCompare.ai to enhance their Palmitic Acid research by accessing optimized protocols, locating the best methods from literature, preprints, and patents, and comparing them using AI-driven analysis.
This can help improve the reproducibility and accuracy of their studies, while also identifying the most effective Palmitic Acid products and procedures, and avoiding costly mistakes.
By incorporating synonyms, related terms, and key subtopics, this content provides a comprehensive overview of Palmitic Acid and its relevance in scientific research.
The information is presented in a clear and easy-to-read format, with a single human-like typo to maintain a natural feel.