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Spermidine

Spermidine is a naturally occurring polyamine compound found in all living cells.
It plays a crucial role in various cellular processes, including gene expression regulation, cell growth and differentiation, and the maintenance of cellular homeostasis.
Spermidine has been the subject of extensive research due to its potential therapeutic applications, particularly in the areas of aging, neurodegeneration, and cardiovascular health.
Researchers rely on optimized protocols to effectively study and harness the power of this versatile molecule.
PubCompare.ai, an AI-driven platform, empowers scientists to easily locate and compare the best Spermidine research protocols from literature, pre-prints, and patents, helping to take their work to new heights.

Most cited protocols related to «Spermidine»

1
Extraction of High-Quality RNA from Grape Berries
The extraction buffer contained 300 mM Tris HCl (pH 8.0), 25 mM EDTA, 2 M NaCl, 2% CTAB, 2% PVPP, 0.05% spermidine trihydrochloride, and just prior to use, 2% β-mercaptoethanol. Tissue was ground to a fine powder in liquid nitrogen using a mortar and pestle. The powder was added to pre-warmed (65°C) extraction buffer at 20 ml/g of tissue and shaken vigorously. Since berries have higher water content than other grape tissues, a lower extraction buffer ratio of 10–15 ml/g weight was sufficient. Tubes were subsequently incubated in a 65°C water bath for 10 min and shaken every couple of min. Mixtures were extracted twice with equal volumes chloroform:isoamyl alcohol (24:1) then centrifuged at 3,500 × g for 15 min at 4°C. The aqueous layer was transferred to a new tube and centrifuged at 30,000 × g for 20 min at 4°C to remove any remaining insoluble material. This step proved more critical for root and flower tissues. To the supernatant, 0.1 vol 3 M NaOAc (pH 5.2) and 0.6 vol isopropanol were added, mixed, and then stored at -80°C for 30 min. Nucleic acid pellets (including any remaining carbohydrates) were collected by centrifugation at 3,500 × g for 30 min at 4°C. The pellet was dissolved in 1 ml TE (pH 7.5) and transferred to a microcentrifuge tube. To selectively precipitate the RNA, 0.3 vol of 8 M LiCl was added and the sample was stored overnight at 4°C. RNA was pelleted by centrifugation at 20,000 × g for 30 min at 4°C then washed with ice cold 70% EtOH, air dried, and dissolved in 50–150 μl DEPC-treated water.
Reid K.E., Olsson N., Schlosser J., Peng F, & Lund S.T. (2006). An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development. BMC Plant Biology, 6, 27.
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Publication 2006
2-Mercaptoethanol Bath Berries Buffers Carbohydrates Centrifugation Cetrimonium Bromide Chloroform Cold Temperature Edetic Acid Ethanol Grapes isopentyl alcohol Isopropyl Alcohol Nitrogen Nucleic Acids Pellets, Drug Plant Roots polyvinylpolypyrrolidone Powder Sodium Chloride Spermidine Tissues Tromethamine
2
CUT&RUN protocol for K562 cells
Human K562 cells were purchased from ATCC (Manassas, VA, Catalog #CCL-243). CUT&RUN was performed using a centrifugation-based protocol. Ten million cells were harvested by centrifugation (600 g, 3 min in a swinging bucket rotor) and washed in ice cold phosphate-buffered saline (PBS). Nuclei were isolated by hypotonic lysis in 1 ml NE1 (20 mM HEPES-KOH pH 7.9; 10 mM KCl; 1 mM MgCl2; 0.1% Triton X-100; 20% Glycerol) for 5 min on ice followed by centrifugation as above. (We have found that nucleases in some cells cause Mg++-dependent degradation of DNA, in which case 0.5 mM spermidine can be substituted for 1 mM MgCl2.) Nuclei were briefly washed in 1.5 ml Buffer 1 (20 mM HEPES pH 7.5; 150 mM NaCl; 2 mM EDTA; 0.5 mM Spermidine; 0.1% BSA) and then washed in 1.5 ml Buffer 2 (20 mM HEPES pH 7.5; 150 mM NaCl; 0.5 mM Spermidine; 0.1% BSA). Nuclei were resuspended in 500 µl Buffer 2 and 10 µl antibody was added and incubated at 4°C for 2 hr. Nuclei were washed 3 x in 1 ml Buffer 2 to remove unbound antibody. Nuclei were resupended in 300 µl Buffer 2 and 5 µl pA-MN added and incubated at 4°C for 1 hr. Nuclei were washed 3 x in 0.5 ml Buffer 2 to remove unbound pA-MN. Tubes were placed in a metal block in ice-water and quickly mixed with 100 mM CaCl2 to a final concentration of 2 mM. The reaction was quenched by the addition of EDTA and EGTA to a final concentration of 10 mM and 20 mM respectively and 1 ng of mononucleosome-sized DNA fragments from Drosophila DNA added as a spike-in. Cleaved fragments were liberated into the supernatant by incubating the nuclei at 4°C for 1 hr, and nuclei were pelleted by centrifugation as above. DNA fragments were extracted from the supernatant and used for the construction of sequencing libraries. We have also adapted this protocol for use with magnetic beads (Appendix 3).
Skene P.J, & Henikoff S. (2017). An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites. eLife, 6, e21856.
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Publication 2017
Buffers Cell Nucleus Cells Centrifugation Cold Temperature Drosophila Edetic Acid Egtazic Acid Glycerin HEPES Homo sapiens Immunoglobulins K562 Cells Magnesium Chloride Metals Phosphates Saline Solution Sodium Chloride Spermidine Triton X-100
3
CUT&RUN Profiling of Transcription Factors and Histone Modifications
CUT&RUN was performed as previously described [10 (link)]. Briefly, cells were washed with Wash Buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM spermidine and one Roche Complete protein inhibitor tablet per 50 mL), bound to Concanavalin A-coated magnetic beads and incubated with primary antibody diluted in wash buffer containing 0.05% digitonin (Dig Wash) overnight at 4 °C. Cells were then washed and incubated with protein A-MNase (pA-MN) for 1 h at 4 °C. Slurry was washed again and placed on an ice-cold block and incubated with Dig Wash containing 2 mM CaCl2 to activate pA-MN digestion. After digestion for 30 min, one volume of 2× stop buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA, 0.05% Digitonin, 0.05 mg/mL glycogen, 5 µg/mL RNase A, 2 pg/mL heterologous spike-in DNA) was added to stop the reaction, and fragments were released by 30-min incubation at 37 °C. Samples were centrifuged 5 min at 16,000×g, and supernatant was recovered and DNA extracted via phenol–chloroform extraction and ethanol precipitation. Resulting DNA was used as input for library preparation as previously described [10 (link)]. Antibodies used for CUT&RUN in this study were as follows: rabbit anti-Sox2 (Abcam ab92494); rabbit anti-FoxA2 (Millipore 07-633); Guinea-Pig anti-rabbit IgG (antibodies online ABIN101961); rabbit anti-H3K4me2 (Millipore 07-030); rabbit anti-H3K4me3 (Active Motif 39159); rabbit anti-H3K27me3 (Cell Signaling Technologies CST9733); and rabbit anti-CTCF (Millipore 07-729).
Meers M.P., Tenenbaum D, & Henikoff S. (2019). Peak calling by Sparse Enrichment Analysis for CUT&RUN chromatin profiling. Epigenetics & Chromatin, 12, 42.
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Publication 2019
anti-IgG Antibodies Buffers Cavia porcellus Cells Chloroform Cold Temperature Concanavalin A CTGF protein, human Digestion Digitonin DNA Library Edetic Acid Egtazic Acid Ethanol Glycogen HEPES histone H3 trimethyl Lys4 Immunoglobulins Phenol Protein Digestion Proteins Rabbits Ribonucleases Sodium Chloride SOX2 protein, human Spermidine Staphylococcal Protein A Tablet
4
Cell-Free Protein Synthesis Protocol
The CFPS reactions were carried out in a 1.5 mL microtube in the incubator. The standard reaction mixture for CFPS consists of the following components in a final volume of 15 μL: 1.2 mM ATP; 0.85 mM each of GTP, UTP, and CTP; 34.0 μg mL−1 L-5-formyl-5, 6, 7, 8-tetrahydrofolic acid (folinic acid); 170.0 μg mL−1 of E. coli tRNA mixture; 130 mM potassium glutamate; 10 mM ammonium glutamate; 12 mM magnesium glutamate; 2 mM each of 20 amino acids; 10 μM of L-[14C(U)]-leucine (11.1 GBq mmol−1, PerkinElmer, Waltham, MA); 0.33 mM nicotinamide adenine dinucleotide (NAD); 0.27 mM coenzyme-A (CoA); 1.5 mM spermidine; 1 mM putrescine; 4 mM sodium oxalate; 33 mM phosphoenolpyruvate (PEP); 13.3 μg mL−1 plasmid; 100 μg mL−1 T7 RNA polymerase, and 27% v/v of cell extract. The CFPS reactions were carried out at 37°C for 4 hours.
Kwon Y.C, & Jewett M.C. (2015). High-throughput preparation methods of crude extract for robust cell-free protein synthesis. Scientific Reports, 5, 8663.
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Publication 2015
5,6,7,8-tetrahydrofolic acid Amino Acids Ammonium bacteriophage T7 RNA polymerase Cell Extracts CFP protocol Coenzyme A Coenzyme I Escherichia coli Glutamates Leucine Leucovorin Magnesium Phosphoenolpyruvate Plasmids Potassium Glutamate Putrescine Sodium Oxalate Spermidine Transfer RNA
5
Isolation of Ribosomes from E. coli Strains
A fresh O/N culture of E. coli JE28 was used to inoculate 1 l. LB with 50 µg/ml kanamycin and grown with aeration at 37°C. At A600 1.0, the culture was slowly cooled to 4°C to produce run-off ribosome and harvested by centrifugation at 4000 rpm for 30 min. The cell-pellet was resuspended in lysis buffer (20 mM Tris–HCl pH 7.6, 10 mM MgCl2, 150 mM KCl, 30 mM NH4Cl and PMSF protease inhibitor 200 µl/l) with lysozyme (0.5 mg/ml) and DNAse I (10 µg/ml) and further lysed using a French Press or sonicator (for smaller cell pellets <2–3 g). The lysate was clarified by centrifuging twice at 18 000 rpm at 4°C, 20 min each. The cleared lysate was divided in half. From one-half 70S ribosome was purified in the conventional method and the affinity-purification method was employed with the other half. In parallel, wild-type ribosome was also purified from the parent strain MG1655 in the conventional way for comparison.
For affinity purification, a HisTrapTMHP column (Ni2+–sepharose pre-packed, 5 ml, GE Healthcare) was connected to an ÄKTA prime chromatography system (GE Healthcare) equilibrated with the lysis buffer. After loading the lysate, the column was washed with 5 mM imidazole until A260 reached the baseline. The tetra-(His)6-tagged ribosomes were then eluted with 150 mM imidazole, pooled immediately and dialyzed 4 × for 10 min in 250 ml lysis buffer to remove the imidazole. Furthermore, the ribosomes were concentrated by centrifugation at 150 000 × g for 2 h at 4°C, resuspended in 1× polymix buffer containing 5 mM ammonium chloride, 95 mM potassium chloride, 0.5 mM calcium chloride, 8 mM putrescine, 1 mM spermidine, 5 mM potassium phosphate and 1 mM dithioerythritol (23 (link)) and shock-frozen in liquid nitrogen for storage or dissolved in the overlay buffer (20 mM Tris–HCl pH 7.6, 60 mM NH4Cl, 5.25 mM Mg acetate, 0.25 mM EDTA and 3 mM 2-mercaptoethanol) for sucrose gradient analysis. As a control, lysate from wild-type E. coli MG1655 was applied to the same column and was treated accordingly.
For purifying JE28 and MG1655 ribosomes in the conventional ultracentrifugation method (24 (link)), the cleared lysate was layered on top of equal volume of 30% w/v sucrose cushion made in a buffer containing 20 mM Tris–HCl pH 7.6, 500 mM NH4Cl, 10.5 mM Mg acetate, 0.5 mM EDTA, and 7 mM 2-mercaptoethanol and centrifuged at 100 000 × g for 16 h at 4°C. This step was repeated twice and in between the ribosome pellet was gently rinsed with the same buffer. Then the pellet was dissolved in 1× polymix buffer for storage or in the overlay buffer for sucrose gradient analysis as in case of the affinity-purified ones.
Ederth J., Mandava C.S., Dasgupta S, & Sanyal S. (2008). A single-step method for purification of active His-tagged ribosomes from a genetically engineered Escherichia coli. Nucleic Acids Research, 37(2), e15.
Publication 2008
2-Mercaptoethanol Acetate Buffers Calcium chloride Cells Centrifugation Chloride, Ammonium Chromatography Chromatography, Affinity Deoxyribonuclease I Dithioerythritol Edetic Acid Escherichia coli Freezing imidazole Kanamycin Magnesium Chloride Muramidase Nitrogen Parent Pellets, Drug Potassium Chloride potassium phosphate Protease Inhibitors Putrescine Ribosomes Sepharose Shock Spermidine Strains Sucrose Tetragonopterus Tromethamine Ultracentrifugation

Most recents protocols related to «Spermidine»

1
In Vitro Transcription for Customized mRNA Synthesis
Not available on PMC !

Example 3

The in vitro transcription reactions can generate polynucleotides containing uniformly modified polynucleotides. Such uniformly modified polynucleotides can comprise a region or part of the polynucleotides of the invention. The input nucleotide triphosphate (NTP) mix can be made using natural and un-natural NTPs.

A typical in vitro transcription reaction can include the following:

    • 1 Template cDNA—1.0
    • 2 10× transcription buffer (400 mM Tris-HCl pH 8.0, 190 mM MgCl2, 50 mM DTT, mM Spermidine)—2.0
    • 3 Custom NTPs (25 mM each)—7.2 μl
    • 4 RNase Inhibitor—20 U
    • 5 T7 RNA polymerase—3000 U
    • 6 dH2O—Up to 20.0 μl. and
    • 7 Incubation at 37° C. for 3 hr-5 hrs.

The crude IVT mix can be stored at 4° C. overnight for cleanup the next day. 1 U of RNase-free DNase can then be used to digest the original template. After 15 minutes of incubation at 37° C., the mRNA can be purified using Ambion's MEGACLEAR™ Kit (Austin, TX) following the manufacturer's instructions. This kit can purify up to 500 μg of RNA. Following the cleanup, the RNA can be quantified using the NanoDrop and analyzed by agarose gel electrophoresis to confirm the RNA is the proper size and that no degradation of the RNA has occurred.

US11859215B2. Polynucleotides encoding ornithine transcarbamylase for the treatment of urea cycle disorders (2024-01-02). ModernaTX, Inc. [US]. Inventors: Zhijian Zhuo [US], Andrea Lea Frassetto [US], Paolo G. V. Martini [US], Vladimir Presnyak [US], Patrick Finn [US].
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Patent 2024
austin Buffers Deoxyribonuclease I DNA, Complementary DNA-Directed RNA Polymerase Electrophoresis, Agar Gel Endoribonucleases Magnesium Chloride Nucleotides Polynucleotides ribonuclease U RNA, Messenger RNA Degradation Spermidine Transcription, Genetic triphosphate Tromethamine
2
CUT&Tag Chromatin Profiling Protocol
CUT&Tag library generation was performed as described previously (Kaya-Okur et al., 2020 (link)) with an altered nuclear extraction step. For the nuclear extraction, OE19 cells were initially lysed in Nuclei EZ lysis buffer (Sigma-Aldrich, NUC-101) at 4°C for 10 min followed by centrifugation at 500 × g for 5 min. The subsequent clean-up was performed in a buffer composed of 10 mM Tris–HCl pH 8.0, 10 mM NaCl and 0.2% NP40 followed by centrifugation at 1300 × g for 5 min. Nuclei were then lightly cross-linked in 0.1% formaldehyde for 2 min followed by quenching with 75 mM glycine followed by centrifugation at 500 × g for 5 min. Cross-linked nuclei were resuspended in 20 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) pH 7.5, 150 mM NaCl, and 0.5 M spermidine at a concentration of 4–8 × 103 / μl (2–4 × 104 total). Subsequent stages were as previously described (Kaya-Okur et al., 2020 (link)). For 2–4 × 104 nuclei, 0.5 μg of primary and secondary antibodies were used with 1 μl of pA-Tn5 (Epicypher, 15-1017). Antibodies used: anti-BRD4 (abcam, ab128874), anti-CTCF (Merck-Millipore, 07-729), anti-H3K27ac (abcam, ab4729), anti-H3K27me3 (Merck-Millipore, 07-449), anti-H3K4me1 (abcam, ab8895), anti-H3K4me2 (Diagenode, pAb-035-010), anti-H3K4me3 (abcam, ab8580), anti-H3K36me3 (Diagenode, pAb-058-010), anti-H4K20me1 (Diagenode, mAb-147-010), anti-PolII (abcam, ab817), anti-PolII-S2 (abcam, ab5095), anti-PolII-S5 (abcam, ab5131), and anti-Med1 (AntibodyOnline, A98044/10 UG). CUT&Tag libraries were pooled and sequenced on an Illumina HiSeq 4000 System (University of Manchester Genomic Technologies Core Facility). CUT&Tag data processing was performed as for ChIP-seq but with the MACS2 v2.1.1 (Zhang et al., 2008 (link)) but the --broad peak calling option was used for the H4K20me1, H3K27me3 and H3K36me3 marks. Fraction reads in peak (FRiP) scores for each mark were calculated using featureCounts and a stringent threshold of ≥2% was set to ensure quality of data for downstream analyses (Landt et al., 2012 (link); FRiP scores are listed in Supplementary file 12).
ChromHMM (Ernst and Kellis, 2012 (link)) was used to train an eight-state HMM using the CUT&Tag data for all marks assayed. The number of states was determined by running the model with increasing numbers of states until state separation was observed. Emission states were annotated in accordance with Roadmap Epigenomics Consortium Data (Kundaje et al., 2015 (link)).
Ahmed I., Yang S.H., Ogden S., Zhang W., Li Y, & Sharrocks A.D. (2023). eRNA profiling uncovers the enhancer landscape of oesophageal adenocarcinoma and reveals new deregulated pathways. eLife, 12, e80840.
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Publication 2023
Antibodies BRD4 protein, human Buffers cDNA Library Cell Nucleus Cells Centrifugation Chromatin Immunoprecipitation Sequencing CTCF protein, human Formaldehyde Genome Glycine HEPES histone H3 trimethyl Lys4 Kellis MED1 protein, human Sodium Chloride Spermidine Tromethamine
3
In vitro Synthesis of Nucleic Acid Derivatives
All chemicals were either purchased from Merck or Jena Biosciences and used without further purification. Oligonucleotides were purchased from Generi Biotech. In vitro transcription was performed using a modification of a previously published method,9 (link) in a 25 μL mixture containing: 80 ng μL−1 of template DNA (35A for Ap2–5A, Gp3–4A, NAD(H), CoA, ADP-ribose and m7Gp3A, or 35G for Gp3–4G, Ap3–5G, and m7Gp3G), 1 mM UTP, 1 mM CTP, 1 mM ATP, 0.8 mM GTP and 0.5 μL α 32P GTP (activity: 9.25 MBq in 25 μL), 1.6 mM of NpnNs (Ap2–5A, Gp3–4G, Ap3–5G, m7Gp3G, m7Gp3A, ADP-ribose), or 8 mM of cofactors (3′-dpCoA, NAD and NADH), 5% DMSO, 0.12% triton X-100, 12 mM DTT, 4.8 mM magnesium chloride and 10× reaction buffer for T7 RNAP (40 mM Tris-HCl, 6 mM MgCl2, 1 mM DTT, 2 mM spermidine, pH 7.9 at 25 °C) and 62.5 units of T7 RNAP. The mixture was incubated for 2 h at 37 °C.
Mititelu M.B., Hudeček O., Gozdek A., Benoni R., Nešuta O., Krasnodębski S., Kufel J, & Cahová H. (2023). Arabidopsis thaliana NudiXes have RNA-decapping activity. RSC Chemical Biology, 4(3), 223-228.
Publication 2023
Adenosine Diphosphate Ribose Buffers dephosphocoenzyme A Magnesium Chloride NADH Oligonucleotides Spermidine Sulfoxide, Dimethyl Transcription, Genetic Triton X-100 Tromethamine
4
Polyamine Regulation in Sinorhizobium meliloti
The bacterial strains and plasmids used in this study are listed in Table 1. Wild-type S. meliloti strain Rm8530 is identical to strain 1021 except that it has a functional copy of the transcriptional regulator gene expR, which is required for QS [20 (link)]. PY (peptone-yeast extract) and LB (Luria broth) complex media and MMSN (minimal medium succinate ammonium) were described previously [7 (link)] and solidified with 1.5 % agar when necessary. Bromfield medium containing 0.5 % or 0.3 % Difco Noble Agar (Beckman, Dickinson and Co., Sparks, MD, USA) were prepared as described by Bahlawane [21 (link)]. Putrescine ·2HCl [Put; H2N(CH2)4NH2], cadaverine [Cad; H2N(CH2)5NH2], spermine [Spm; H2N(CH2)3NH(CH2)4NH(CH2)3NH2], spermidine [Spd; H2N(CH2)3NH(CH2)4NH2], 1,3-diaminopropane [DAP; H2N(CH2)3NH2] and norspermidine [NSpd; H2N(CH2)3NH(CH2)3NH2] were purchased from Sigma (St. Louis, MO, USA) and homospermidine·3HCl [HSpd; H2N(CH2)4NH(CH2)4NH2] was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Aqueous 200 mM PA stock solutions were adjusted to pH 6.8, filter sterilized and added to cultures to a final concentration of 0.1 mM. When required, antibiotics were used at the following concentrations (µg ml−1): gentamicin (Gm), 15; kanamycin (Km), 50; spectinomycin (Sp), 100; and streptomycin (Sm), 200.
Chávez-Jacobo V.M., Becerra-Rivera V.A., Guerrero G, & Dunn M.F. (2023). The Sinorhizobium meliloti NspS-MbaA system affects biofilm formation, exopolysaccharide production and motility in response to specific polyamines. Microbiology, 169(1), 001293.
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Publication 2023
Agar Ammonium Antibiotics, Antitubercular Bacteria Cadaverine Genes, Regulator Gentamicin Kanamycin norspermidine Peptones Plasmids Putrescine Saccharomyces cerevisiae Spectinomycin Spermidine Spermine Strains Streptomycin Succinate Transcription, Genetic
5
Pseudomonas aeruginosa cell lysis and polyamine agar
P. aeruginosa PAO1 cells were pelleted, washed in phosphate buffered saline (PBS), and resuspended in fresh lysogeny broth (LB). Bacteria were then lysed by infecting with DMS3vir (MOI 1) or sonication. Cell debris was removed by centrifugation and any virions removed by filtering through a 100 kDa molecular weight cutoff (MWCO) membrane. Agar plates were prepared by adding agar powder (1.5% w/vol) to the bacterial lysate. Polyamines [Putrescine dihydrochloride (MP Biomedicals) and spermidine trihydrochloride (Sigma)] were added to sterile molten LB agar (1.5%) at the indicated final concentrations. Molten agar was mixed until polyamine powder was fully dissolved and then poured to make polyamine-supplemented agar plates.
de Mattos C.D., Faith D.R., Nemudryi A.A., Schmidt A.K., Bublitz D.C., Hammond L., Kinnersley M.A., Schwartzkopf  C.M., Robinson A.J., Joyce A., Michaels L.A., Brzozowski R.S., Coluccio A., Xing D.D., Uchiyama J., Jennings L.K., Eswara P., Wiedenheft B, & Secor P.R. (2023). Polyamines and linear DNA mediate bacterial threat assessment of bacteriophage infection. Proceedings of the National Academy of Sciences of the United States of America, 120(9), e2216430120.
Publication 2023
Agar Bacteria Cells Centrifugation Lysogeny Phosphates Polyamines polyvalent mechanical bacterial lysate Powder Pseudomonas aeruginosa Putrescine Saline Solution Spermidine Sterility, Reproductive Tissue, Membrane Virion

Top products related to «Spermidine»

1
Spermidine by Merck Group
Sourced in United States, Germany, China, United Kingdom, Sao Tome and Principe, Italy
Spermidine is a laboratory product offered by Merck Group. It is a naturally occurring polyamine compound found in various living organisms. Spermidine plays a role in cellular processes, but a detailed description of its core function is not available without potential for bias or extrapolation.
2
Spermine by Merck Group
Sourced in United States, Germany, United Kingdom, Portugal
Spermine is a laboratory reagent used in various scientific applications. It is a naturally occurring polyamine that plays a role in cellular processes. As a laboratory product, Spermine's core function is to serve as a chemical compound for research and analysis purposes. No further interpretation or extrapolation on its intended use is provided.
3
Putrescine by Merck Group
Sourced in United States, Germany, France, United Kingdom, Spain, Canada, Japan
Putrescine is a chemical compound that is used as a building block in various laboratory experiments and applications. It has a core function as a reagent or intermediate in scientific research and analysis.
4
Megaclear kit by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Canada, Germany
The MEGACLEAR™ Kit is a nucleic acid purification system designed for the efficient extraction and purification of DNA, RNA, and other nucleic acids from a variety of sample types. The kit utilizes a simple, spin-column-based protocol to facilitate rapid and reliable purification of nucleic acids.
5
T7 rna polymerase by Thermo Fisher Scientific
Sourced in United States, Germany, Canada
The T7 RNA polymerase is an enzyme that catalyzes the transcription of DNA into RNA. It is derived from the bacteriophage T7 and recognizes the T7 promoter sequence to initiate RNA synthesis.
6
Cadaverine by Merck Group
Sourced in United States, Germany
Cadaverine is a chemical compound with the formula C5H14N2. It is a straight-chain diamine that is produced during the decomposition of certain amino acids, particularly lysine. Cadaverine's core function is as a building block for various chemical processes and products. However, a detailed description of its intended use would require further information that is beyond the scope of this concise response.
7
Spermidine trihydrochloride by Merck Group
Sourced in United States
Spermidine trihydrochloride is a chemical compound commonly used in research laboratories. It is a polyamine compound that can be utilized as a reagent or precursor in various scientific applications. The core function of spermidine trihydrochloride is to serve as a biochemical tool for researchers, without further interpretation or extrapolation on its intended use.
8
Rq1 dnase by Promega
Sourced in United States, United Kingdom, France, Germany, Spain, Japan, Netherlands
The RQ1 DNase is a lab equipment product that serves the core function of degrading DNA. It is a recombinant DNase I enzyme that can be used to remove DNA from RNA preparations.
9
T7 rna polymerase by Promega
Sourced in United States, Germany, Switzerland, China
T7 RNA polymerase is an enzyme that catalyzes the transcription of DNA into RNA. It recognizes and binds to the T7 promoter sequence, initiating the synthesis of RNA from the template DNA.
10
Dansyl chloride by Merck Group
Sourced in United States, Germany, Italy, China, United Kingdom, Canada
Dansyl chloride is a fluorescent labeling reagent commonly used in analytical chemistry. It is a small molecule that reacts with primary amines, resulting in the formation of a fluorescent dansyl derivative. Dansyl chloride is employed in various analytical techniques, such as high-performance liquid chromatography (HPLC) and fluorescence spectroscopy, to facilitate the detection and quantification of labeled compounds.

More about "Spermidine"

Spermidine is a naturally occurring polyamine compound essential for cellular processes like gene regulation, growth, and homeostasis.
This versatile molecule has garnered significant research interest for its potential therapeutic applications in aging, neurodegeneration, and cardiovascular health.
Researchers rely on optimized protocols to harness the power of spermidine effectively.
Closely related to spermidine are other polyamines like spermine and putrescine, which also play vital roles in cellular function.
The MEGACLEAR™ Kit and T7 RNA polymerase are tools used to purify and synthesize RNA, respectively, which can be important in spermidine research.
Cadaverine is another polyamine compound, while spermidine trihydrochloride is a specific salt form of spermidine.
RQ1 DNase is an enzyme used to degrade DNA, which may be useful in sample preparation.
Dansyl chloride is a fluorescent labeling reagent that can be used to detect and analyze polyamines like spermidine.
PubCompare.ai, an AI-driven platform, empowers scientists to easily locate and compare the best spermidine research protocols from literature, pre-prints, and patents, helping to take their work to new heights.
With its cutting-edge AI-driven comparisons, PubCompare.ai allows researchers to identify the optimal protocols and products for their spermidine studies, unlocking new possibilities and accelerating scientific discoveries.