The profile-based detection of secondary metabolite clusters has now been augmented by a tighter integration of the generalized PFAM (22 (link)) domain-based ClusterFinder algorithm (Cimermancic et al., in preparation) already included in version 1.0 of antiSMASH. This algorithm performs probabilistic inference of gene clusters by identifying genomic regions with unusually high frequencies of secondary metabolism-associated PFAM domains, and it was designed to detect ‘classical’ as well as less typical and even novel classes of secondary metabolite gene clusters. While antiSMASH 1.0 only generated the output of this algorithm in a static image, version 2.0 displays these additional putative gene clusters along with the other gene clusters in the HTML output. A key advantage of this is that these putative gene clusters will now also be included in the subsequent (Sub)ClusterBlast analyses.
Terpenes
They are characterized by their distinct aromatic properties and have been the subject of increasing scientific interest due to their potential therapeutic applications.
Terpenes exhibit a wide range of biological activities, including anti-inflammatory, analgesic, and antimicrobial effects.
This MeSH term provides a comprehensive overview of the chemical structure, biosynthesis, and pharmacological properties of terpenes, enabling researchers to explore their role in various areas of medicine and biolodycs.
Most cited protocols related to «Terpenes»
The profile-based detection of secondary metabolite clusters has now been augmented by a tighter integration of the generalized PFAM (22 (link)) domain-based ClusterFinder algorithm (Cimermancic et al., in preparation) already included in version 1.0 of antiSMASH. This algorithm performs probabilistic inference of gene clusters by identifying genomic regions with unusually high frequencies of secondary metabolism-associated PFAM domains, and it was designed to detect ‘classical’ as well as less typical and even novel classes of secondary metabolite gene clusters. While antiSMASH 1.0 only generated the output of this algorithm in a static image, version 2.0 displays these additional putative gene clusters along with the other gene clusters in the HTML output. A key advantage of this is that these putative gene clusters will now also be included in the subsequent (Sub)ClusterBlast analyses.
Gene clusters are defined by locating clusters of signature gene pHMM hits spaced within <10 kb mutual distance. To include flanking accessory genes, gene clusters are extended by 5, 10 or 20 kb on each side of the last signature gene pHMM hit, depending on the gene cluster type detected. As a consequence of this greedy methodology, gene clusters that are spaced very closely together may be merged into ‘superclusters’. These gene clusters are indicated in the output as ‘hybrid clusters’; they may either represent a single gene cluster which produces a hybrid compound that combines two or more chemical scaffold types, or they may represent two separate gene clusters which just happen to be spaced very closely together.
Cheminformatic metrics, including molecular weight, number of hydrogen bond donors and acceptors, octanol-water partition coefficients, and Bertz topological complexity, were calculated in RDKit. Both platforms occasionally generated very small, non-specific structure predictions (for example, a single unspecified amino acid or a single malonyl unit) that did not provide actionable information about the chemical structure of the encoded product; to remove these from consideration, we applied a molecular weight filter to remove structures under 100 Da output by either platform. To evaluate the internal structural diversity of each set of predicted structures, we computed the distribution of pairwise Tcs for each set45 , taking the median pairwise Tc instead of the mean as a summary statistic to ensure robustness against outliers. Structural similarity to known natural products was assessed using the RDKit implementation of the ‘natural product-likeness’ score22 (link), and by the median Tc between predicted structures and the known secondary metabolite structures deposited in the NP Atlas database46 (link).
Tannins. 200 mg of plant material was boiled in 10 mL distilled water and few drops of FeCl3 were added to the filtrate; a blue-black precipitate indicated the presence of Tannins.
Alkaloids. 200 mg plant material was boiled in 10 mL methanol and filtered. 1% HCl was added followed by 6 drops of Dragendorff reagent, and brownish-red precipitate was taken as evidence for the presence of alkaloids.
Saponins (Frothing test). 5 mL distilled water was added to 200 mg plant material. 0.5 mL filtrate was diluted to 5 mL with distilled water and shaken vigorously for 2 minutes. Formation of stable foam indicates the presence of saponins.
Cardiac Glycosides (Keller-Kiliani test). 2 mL filtrate was treated with 1 mL glacial acetic acid containing few drops of FeCl3.Conc. H2SO4 was added to the above mixture giving green-blue colour depicting the positive results for presence of cardiac glycosides.
Steroids (Liebermann-Burchard reaction). 200 mg plant material was added in 10 mL chloroform. Acetic anhydride was added in the ratio of 1 : 1 which resulted into the formation of blue-green ring pointing towards the presence of steroids.
Terpenoids (Salkowski test). To 200 mg plant material 2 mL of chloroform (CHCl3) and 3 mL of concentrated sulphuric acid (H2SO4) were carefully added. A reddish brown colouration signified the presence of terpenoids.
Flavonoids. To the aqueous filtrate 5 mL of dilute ammonia solution was added, followed by concentrated H2SO4. A yellow colouration indicated the presence of flavonoids.
Phlobatannins. The deposition of a red precipitate denoted the presence of phlobatannins when 200 mg of plant material was dissolved in 10 mL of aqueous extract and few drops of 1% HCl were added in the boiling tube.
Anthraquinones. 500 mg of dried plant leaves were boiled in 10% HCl for 5 mins and filtrate was allowed to cool. Equal volume of CHCl3 with few drops of 10% NH3 was added to 2 mL filtrate. The formation of rose-pink colour implies the presence of Anthraquinones.
Reducing Sugars. To the 10 mL of aqueous extract a few drops of Fehling's solution A and B were added; an orange red precipitate suggests the presence of reducing sugars.
Most recents protocols related to «Terpenes»
Example 5
Dehydrogenation of terpenes to cymene was evaluated in the fixed bed reactor system depicted in
Example 1
A pharmaceutical composition was prepared as described below. The following products were used in the amounts and concentrations specified:
-
- 1. About 20 g cannabinoid distillate
- 2. About 35 g Ethanol 95%
- 3. About 40 g maltodextrin/gum acacia mixture
- The cannabinoid distillate was weighed in a glass beaker. Ethanol 95% was added to the same beaker. The contents of the beaker were allowed to dissolve on a hot plate set to 55° C.
The above solution was combined with the maltodextrin/gum acacia in a planar mixer and was gently mixed until well incorporated.
The above mixture was passed through a granulation screen into a second bowl. This bowl was placed into a vacuum oven at 55° C. for 12 hours. The powder was stirred at least one during this time frame.
The formulation above was tested for potency and stability after 1 year of storage. After this period, no loss of potency was observed (as measured by HPLC), the formulation was visibly stable at room temperature and readily fluid when shaken.
Heatmap showing the 91 partly annotated variables selected by the normalized BORUTA. The y-axis displays the clustering of samples and the x-axis displays the clustering of selected features. R2 = 0.75, RMSE = 1.095445, MAE = 0.8. Black boxes were drawn to better differentiate the shifts in the samples. Blue indicates that a feature was produced less than the control and red indicates that a feature was produced more than the control
Molecular network showing the relationships of the selected compounds to pathways and compound classes. More information regarding the selected compounds is available in the Supplementary Material
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During the myopia induction phase, mice were given either normal (MF, Oriental Yeast Co., Ltd, Tokyo, Japan) or mixed chow containing the candidate chemical 0.0667 percent GBEs (INDENA JAPAN CO., Tokyo, Japan #9,033,008). 0.0667% GBEs contain 24% of the flavonol glycosides of quercetin, kaempferol, and isorhamnetin and 6% terpene trilactones. The corresponding concentration of GBEs mixed chow was 200 mg/kg/day, which is consistent with the concentration of GBEs that causes the significantly high activity of EGR-1 in vitro experiments. The addition of GBEs and the production of 0.0667% GBEs mixed chow are all produced by chow manufacturing company (Oriental Yeast Co., LTD., Tokyo, Japan).
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More about "Terpenes"
They are characterized by their distinct aromatic properties and have been the subject of increasing scientific interest due to their potential therapeutic applications.
Terpenes exhibit a wide range of biological activities, including anti-inflammatory, analgesic, and antimicrobial effects.
This comprehensive overview covers the chemical structure, biosynthesis, and pharmacological properties of terpenes, enabling researchers to explore their role in various areas of medicine and biology.
Synonyms for terpenes include isoprenoids, terpenoids, and essential oil constituents.
Subtopics related to terpenes include: - Limonene: A monocyclic monoterpene found in citrus fruits with potential anti-cancer and anti-inflammatory properties. - Linalool: A monoterpene alcohol found in many plants, such as lavender, with reported sedative, analgesic, and anti-anxiety effects. - α-Pinene and β-Pinene: Bicyclic monoterpenes with potential antimicrobial, anti-inflammatory, and bronchodilatory activities. - Myrcene: A monoterpene with anti-inflammatory, analgesic, and sedative effects, often found in cannabis and other plants.
Experimental techniques used in terpene research include: - HP-5MS column: A common gas chromatography (GC) column used for the separation and analysis of terpenes and other volatile compounds. - Whatman No. 1 filter paper: A type of filter paper often used in the extraction and purification of terpenes from plant materials. - DMSO and Ethanol: Solvents commonly used in the extraction and solubilization of terpenes for various applications.
By understanding the diverse properties and applications of terpenes, researchers can optimize their experiments and unlock new insights in the fields of medicine, biolodycs, and beyond.