3-(triethoxysilyl)propylamine
It is a silane-based reagent that can be used to introduce functional groups onto surfaces or into polymeric materials.
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Most cited protocols related to «3-(triethoxysilyl)propylamine»
2. Drop 18–22 μl of fixative (1:1, 2:1, 3:1 or 5:1 ethanol:acetic acid)** and wait till the surface becomes granule-like and the layer of fixative becomes thin (25–35 sec)
3. Put the slides upside down under the steam from a water bath at 55°C (10–15 cm from water surface of the water bath) for 3–5 sec
4. For double “SteamDrop”, repeat step 2 but with less volume (3–6 μl) of fixative and higher concentration of acetic acid. Perform Step 3 for 1 sec only.
5. Immediately dry slides with air flow (e.g. a tabletop fan).
Note
*-for preparation of large size chromosomes it is useful to coat slides with APES (3-aminopropyl-triethoxy-silane) to prevent a chromosome partial detachment. APES coating of slides: 1.5% APES in 100% acetone for 30 sec, twice wash in distilled water and dry for 1 h at 37°C.
**– the protocol allows an easy correction of enzyme treatment results - check the level of tissue enzymatic digestion in the first chromosome preparation slide under microscope, if tissue is underdigested use a high proportion of acetic acid in fixative (1:1 or 2:1); if tissue is overdigested use a low proportion of acetic acid in fixative (5:1 or 10:1).
Most recents protocols related to «3-(triethoxysilyl)propylamine»
Example 1
A nitrogen-purged autoclave reactor was charged with cyclohexane, tetrahydrofuran, styrene, and 1,3-butadiene. The temperature of the contents of the reactor was adjusted to 20° C., and then n-butyllithium was added to initiate polymerization. The polymerization was carried out under adiabatic conditions, and the maximum temperature reached 85° C. Once the polymerization conversion ratio reached 99%, 1,3-butadiene was further added, followed by polymerization for five minutes. Subsequently, N,N-bis(trimethylsilyl)-3-aminopropyltriethoxysilane was added as a modifier, and a reaction was performed. After completion of the polymerization reaction, 2,6-di-tert-butyl-p-cresol was added. Thereafter, the solvent was removed by steam stripping. The product was dried on hot rolls adjusted at 110° C. to obtain SBR 1.
GMPCPP-bound tubulin labeled with Alexa Fluor 488 (2% labeled) and biotin (5% labeled) was incubated at 27°C for 1 h to promote nucleation, followed by 1 h of polymerization at 37°C. Polymerized microtubule samples were purified by centrifugation at 20,000g, 35°C for 25 min.
The glass chamber was treated as follows: first, 1 mg/ml NeutrAvidin was loaded into the chamber and incubated for 2 min to let it bind to biotin molecules covalently connected to the glass surface. The glass surface was subsequently deactivated by adding and incubating with 10× blocking solution (1 mg/ml casein and 1% pluronic F127) for 2 min. After washing them out with BRB80 containing 25% glycerol, GMPCPP-MT seeds were loaded into the chamber and incubated for 2 min to immobilize microtubules on the glass surface. Unbound microtubules were washed out with BRB80 containing 10x blocking solution and 1 mM GTP. To polymerize GDP-MT from the seeds, 28 μM Cy5-labeled tubulin (labeling rate 2%) in polymerization buffer (BRB80, 2× blocking solution, 1 mM GTP) was applied to the chamber, polymerized for 5 min, and terminated by washing free tubulin out with washout buffer (BRB80, 25% glycerol, and 2× blocking solution). To completely hydrolyze GTP, the chamber was incubated for more than 30 min before any subsequent experiments.
were prepared by our previously described salt-induced self-assembly
approach.43 (link),44 (link) Briefly, 100% clottable fibrinogen from
human plasma (Merck, Darmstadt, Germany), dissolved in 10 mM NH4HCO3 (Carl Roth GmbH, Karlsruhe, Germany) in deionized
H2O, was dialyzed overnight against the same solution using
a cellulose membrane dialysis tube (cutoff 14 kDa; Sigma-Aldrich,
Darmstadt, Germany) to obtain a fibrinogen solution. Fifteen millimeter
glass coverslips (VWR, Darmstadt, Germany) were cleaned with piranha
solution (3:1 of 95% sulfuric acid (H2SO4)/30%
hydrogen peroxide (H2O2)) before treatment with
5% (3-aminopropyl)triethoxysilane (APTES, Sigma) in ethanol (C2H5OH) overnight. Unbound APTES was removed by washing
in pure ethanol three times for 5 min, before storing the coverslips
dry for further use.
To obtain fibrinogen nanofibers, 5 mg mL–1 of the fibrinogen solution was dried in the presence
of 2.5× phosphate-buffered saline (PBS, ThermoFisher, pH 7.4)
on APTES-modified coverslips. Planar fibrinogen scaffolds were obtained
by drying 5 mg mL–1 of fibrinogen in 5 mM NH4HCO3. Drying was performed in a custom-built climate
chamber at a relative humidity of 30% and temperature of 24 °C for 12 h.
To maintain the stability
of scaffolds in an aqueous environment
for further cell culture studies, the scaffolds were cross-linked
in formaldehyde (FA) vapor for 2 h after placing them in a sealed
beaker. The FA vapor was generated by placing 1 μL of 37% FA
solution (Applichem GmbH, Darmstadt, Germany) per cubic centimeter
and letting it evaporate in the sealed beaker. After cross-linking
and devaporizing for another 30 min, all samples were washed 3 × 15 min with deionized water. We have extensively
characterized the nanofibrous topography with dense, porous nanofiber
networks of these fibrinogen nanofiber scaffolds as well as the smooth
topography of the planar fibrinogen scaffolds previously.43 (link),45 (link)To obtain un-cross-linked adsorbed fibrinogen substrates,
the so-called
physisorbed fibrinogen scaffolds, a slightly modified protocol from
a previously published method was used.46 (link) Fifteen millimeter glass coverslips (VWR) were cleaned with piranha
solution (3:1 of 95% sulfuric acid (H2SO4)/30%
hydrogen peroxide (H2O2)). A 0.1 mg mL–1 fibrinogen solution in PBS (Thermo Fisher, pH 7.4) was then dried
on the piranha-cleaned coverslips at 4 °C overnight. Subsequently,
the scaffolds were washed three times for 5 min with PBS. The physisorbed
fibrinogen scaffolds displayed a flat and smooth topography (see
earlier.46 (link)Nanofibrous, planar, as
well as physisorbed fibrinogen scaffolds
were placed in wells of nontreated Corning Costar 24-well plates (Sigma)
and sterilized for 30 min using the UV light of a laminar flow cabinet
(ESI Flufrance) to further use them in cell culture experiments.
the monolithic packing, the polyimide-coated fused-silica capillaries
(100 μm i.d., 375 μm o.d.) from Polymicro Technologies
(Phoenix, AZ, USA) were first treated by an adapted etching protocol28 (link) at room temperature comprising a rinse with
methanol followed by deionized water, then etching with aqueous 1
M NaOH for 2 h followed by a rinse with deionized water until neutral.
This was followed by flushing with 1 M aqueous HCl for 20 min, deionized
water until neutral, methanol for 1 h, and drying by a flow of nitrogen
at room temperature for 12 h. These etched capillaries were subsequently
modified to produce three different activated capillaries (
the Supporting Information), first by 10% (v/v) (3-aminopropyl)triethoxysilane
(APTES) in toluene at 60 °C overnight, followed by washing with
water and methanol and drying as in the final step of the above etching
procedure, yielding capillaries
were subsequently reacted according to two different schemes; capillaries
and reacting at 80 °C for 4 h, followed by washing and drying
as above; capillaries
(see below) diluted 1 + 9 (v/v) with water and reacted at 80 °C
for 4 h, followed by washing and drying as above. Activated capillaries
were stored in a desiccator for use in the polymerization of MF monolithic
columns.
#1 filter paper (47 mm in diameter) and dried in a convection oven
at 60 °C for 1 h (
of a 10 mM sodium borohydride (NaBH4, MERCK) aqueous solution
for an additional hour. Au ions settled on the filter paper were reduced
to AuNPs via a reduction reaction with NaBH4, resulting
in an instantaneous color change from white to purple. The paper containing
the AuNPs, labeled Paper_AuNPs, was then rinsed with deionized water
and ethanol to remove any residual reagents and dried in a convection
oven at 60 °C. The Paper_AuNPs film was submerged in 10 mL of
toluene containing 5% 3-aminopropyltriethoxysilane (APTES, 99%, MERCK)
and placed in a 30 °C, 100 rpm, shaking incubator for 1 day.
The paper was then rinsed with toluene and dried in a convection oven
to remove any residual APTES; it was then labeled as Paper_Si_AuNPs.
The Paper_Si_AuNPs were then immersed in 10 mL of ethanol containing
5% trans-cinnamaldehyde (CIN, 99%, MERCK) and placed
in a 50 °C, 100 rpm incubator for 1 day. The Paper_AuNPs_CIN
film was fabricated by connecting amine groups on the outer surface
of the Paper_Si_AuNPs film to trans-cinnamaldehyde
(CIN, 99%, MERCK) via an imine (C=N) bond. The fabrication of Paper_AuNPs_CIN
was completed by a drying procedure following three 5 min ultrasonication
treatments with ethanol. The paper CIN was produced using the identical
method without AuNPs reduction (
Top products related to «3-(triethoxysilyl)propylamine»
More about "3-(triethoxysilyl)propylamine"
It is also known as 3-aminopropyltriethoxysilane (APTES) and can be used to introduce functional groups onto surfaces or into polymeric materials.
Researchers optimizing their 3-(triethoxysilyl)propylamine research can utilize PubCompare.ai's AI-driven reproducibility platform to easily locate protocols from literature, preprints, and patents.
The platform's AI-driven comparisons can help identify the best protocols and products for their experiments, streamlining the research process and ensuring reliable results.
APTES is often used in conjunction with other silane-based reagents, such as tetraethyl orthosilicate (TEOS), to create functionalized surfaces or to modify the properties of polymeric materials.
It can also be used in combination with compounds like hydrochloric acid, sodium hydroxide, and ethanol for various surface treatment and modification applications.
In addition to surface modification, 3-(triethoxysilyl)propylamine has applications in the preparation of bioactive materials, such as those involving bovine serum albumin (BSA) and N-hydroxysuccinimide (NHS).
These materials can be used in a variety of biomedical and biotechnological applications, including drug delivery, tissue engineering, and biosensing.
Researchers can streamline their 3-(triethoxysilyl)propylamine research and achieve reliable results by utilizing PubCompare.ai's AI-driven reproducibility platform, which helps locate the best protocols and products for their experiments.
This can be particularly useful when working with APTES and related silane-based compounds, as well as in the development of bioactive materials involving DMSO and other solvents.