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3-(triethoxysilyl)propylamine

3-(triethoxysilyl)propylamine is a chemical compound used in a variety of applications, including surface modification, polymer synthesis, and materials science.
It is a silane-based reagent that can be used to introduce functional groups onto surfaces or into polymeric materials.
Researchers can optimize their 3-(triethoxysilyl)propylamine research with PubCompare.ai's AI-driven reproducibility platform, which helps locate protocols from literature, preprints, and patents, and provides AI-driven comparisons to identify the best protocols and products for their experiments.
This can help streamline research and acheive reliable results.

Most cited protocols related to «3-(triethoxysilyl)propylamine»

Two flowers per day from anthesis, two and three days after pollination were fixed in 4% formaldehyde freshly prepared from paraformaldehyde in 1x phosphate saline buffer (PBS) pH7.3, left overnight at 4ºC, and conserved then at 0.1% formaldehyde solution [83 (link)]. Then the pistils were dehydrated in an acetone series (30%, 50%, 70%, 90%, 100%), and embedded in Technovit 8100 (Kulzer and Co, Germany) for two days. The resin was polymerized at 4ºC, and sectioned at 4 μm thickness. Sections were placed in a drop of water on a slide covered with 2% (3-Aminopropyl) triethoxysilane - APTEX (Sigma-Aldrich), and dried at room temperature. Callose was identified with the anticallose antibody (AntiCal) that recognises linear β-(1,3)-glucan segments (anti-β-(1,3)-glucan; immunoglobulin G1), Biosupplies, Australia [49 (link)]. As a secondary antibody, Alexa 488 fluorescein isothiocyanate (FITC)-conjugated anti-mouse IgG was used (F-1763; Sigma). Additionally, a monoclonal antibody (mAbs) JIM13 [84 (link)] against AGPs glycosyl epitopes, and one mAb JIM11 [85 (link)] against extensin epitopes were obtained from Carbosource Services (University of Georgia, USA). Secondary antibodies were anti-rat IgG conjugated with the same Alexa 488 used above. Sections were incubated for 5 min in PBS pH7.3 followed by 5% bovine serum albumin (BSA) in PBS for 5 min. Then, sections were incubated at room temperature for 1h with AntiCal primary mAb, JIM13, and JIM11. After that, three washes in PBS of 5 minutes each preceded the incubation for 45 min in the dark with a 1/25 diluted secondary fluorescein isothiocyanate (FITC) conjugated with the antibody in 1% BSA in PBS, followed by three washes in PBS [83 (link)]. Sections were counterstained with calcofluor white for cellulose [86 (link)], mounted in PBS or Mowiol, and examined under a LEICA DM2500 epifluorescence microscope connected to a LEICA DFC320 camera. Filters were 355/455 nm for calcofluor white and 470/525 nm for the Alexa 488 fluorescein label of the antibodies (White Level?=?255; Black Level = 0; ϒ?=?1). Exposur (Exp) times were adapted to the best compromise in overlapping photographs for each antibody: AntiCal, Exp.?=?15.30ms (Calcofluor Exp. = 1.20ms); JIM13 Exp.?=?2.52ms (Calcofluor?=?0.41ms); JIM11, Exp. = 31.59 ms (Calcofluor Exp. = 1.40ms). Brightness and contrasts were adjusted to obtain the sharpest images with the Leica Application Suite software.
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Publication 2014
3-(triethoxysilyl)propylamine Acetone anti-IgG Antibodies Bos taurus Buffers calcofluor white callose Cellulose Contrast Media Epitopes Flowers Fluorescein Formaldehyde Formalin Glucans Immunoglobulins isothiocyanate Mice, House Microscopy Orosomucoid paraform Phosphates Pistil Pollination Resins, Plant Saline Solution Serum Albumin Serum Albumin, Bovine

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Publication 2009
3-(triethoxysilyl)propylamine Alexa 350 alexa 568 alexa fluor 488 Alkalies Antibodies Antibodies, Anti-Idiotypic Avidin Biological Assay Biotin Buffers Cells Cloning Vectors Ethanol Glutaral Goat Immunoglobulins isolation Mice, House Microscopy Molecular Probes Monoclonal Antibodies neutravidin Nucleotides Pulses Telomere
CNS and PNS tissues were fixed by transcardial perfusion with 4% paraformaldehyde in 0.1 M sodium phosphate, pH 7.4. After extensive washing with 0.1 M sodium phosphate, pH 7.4, the tissue was cryoprotected by immersion for 15 min each in 5%, then 10% sucrose in 0.1 M phosphate buffer, pH 7.4, and finally overnight in 25% sucrose in 0.1 M phosphate buffer, pH 7.4, at 4°C. After embedding in OCT (Tissue TEK) blocks were frozen in isopentane cooled in liquid nitrogen. Sections (8–10 μm) were collected on 3-aminopropyltriethoxysilane subbed glass slides, and OCT was removed by washing in PBS (Sigma Chemical Co.). Sciatic nerve fibers were teased in 0.1% Triton X-100 in PBS. Before incubation with the NFF3 antibody, sections or teased sciatic nerve fibers were treated with Bouin's reagent for 1 min. Samples were blocked for 3 h in 0.2% Triton X-100, 0.2% gelatin in PBS, pH 7.4, containing either 10% nonimmune goat serum when using rabbit and mouse primary antibodies, or donkey serum when the sheep antibody was used. The antibodies were applied overnight in 4% nonimmune serum in the same buffer, and after washing them in buffer without serum, fluorescently labeled secondary antibodies were applied for 2 h in buffer containing serum, followed by further washes. Secondary antibodies were as follows: FITC-labeled goat anti–rabbit (Cappel Laboratories), TRITC-labeled goat anti–mouse IgG1, TRITC-labeled donkey anti–rabbit (Jackson Laboratories), and FITC-labeled donkey anti–sheep (Jackson Laboratories). Further washes in blocking buffer minus serum were followed by several washes in PBS. Sections were mounted in Vectashield (Vector Laboratories). Images were captured with a Leica TCS4D confocal or Olympus BX60 microscope, and figures were produced with Adobe Photoshop.
Publication 2000
3-(triethoxysilyl)propylamine Antibodies Buffers Cardiac Arrest Cloning Vectors Equus asinus Fluorescein-5-isothiocyanate Freezing Gelatins Goat IgG1 Immunoglobulins isopentane Mice, House Microscopy Nerve Fibers Nitrogen paraform Perfusion Phosphates Rabbits Serum Sheep sodium phosphate Submersion Sucrose tetramethylrhodamine isothiocyanate Tissues Triton X-100
1. Drop 10 μl of cell suspension onto a slide* and wait till the surface becomes granule-like, i.e. ethanol meniscus occurred on the top of the cells, (10–15 sec)
2. Drop 18–22 μl of fixative (1:1, 2:1, 3:1 or 5:1 ethanol:acetic acid)** and wait till the surface becomes granule-like and the layer of fixative becomes thin (25–35 sec)
3. Put the slides upside down under the steam from a water bath at 55°C (10–15 cm from water surface of the water bath) for 3–5 sec
4. For double “SteamDrop”, repeat step 2 but with less volume (3–6 μl) of fixative and higher concentration of acetic acid. Perform Step 3 for 1 sec only.
5. Immediately dry slides with air flow (e.g. a tabletop fan).
Note
*-for preparation of large size chromosomes it is useful to coat slides with APES (3-aminopropyl-triethoxy-silane) to prevent a chromosome partial detachment. APES coating of slides: 1.5% APES in 100% acetone for 30 sec, twice wash in distilled water and dry for 1 h at 37°C.
**– the protocol allows an easy correction of enzyme treatment results - check the level of tissue enzymatic digestion in the first chromosome preparation slide under microscope, if tissue is underdigested use a high proportion of acetic acid in fixative (1:1 or 2:1); if tissue is overdigested use a low proportion of acetic acid in fixative (5:1 or 10:1).
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Publication 2014
3-(triethoxysilyl)propylamine Acetic Acid Acetone APEX1 protein, human Bath Cells Chromosomes Cytoplasmic Granules Digestion Enzymes Ethanol Fixatives Meniscus Microscopy Pongidae Steam Test, Clinical Enzyme Tissues
Silicon wafers (100 orientation, P/B doped, resistivity 1–10 Ω-cm, thickness 475–575 μm) were purchased from International Wafer Service. Silane coupling agents, 3-aminopropyltriethoxysilane (APTES, 99.7%), 3-aminopropyltrimethoxysilane (APTMS, 98.5%), N-(6-aminohexyl)aminomethyltriethoxysilane (AHAMTES, 99.7%), N-(2-aminoethyl)-3-aminopropyltriethoxysilane (AEAPTES, 97.4%), and N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (AEAPTMS, 98.3%), were purchased from Gelest. The purity information was provided by the manufacturer. The silanes were stored in Schlenk flasks under nitrogen and used without further distillation. House-purified water (reverse osmosis) was purified in a Millipore Milli-Q Biocell System (Millipore) that involved reverse osmosis, ion exchange, and filtration (18.2 MΩ-cm). Other reagents were used as received from Fisher Scientific. Solution-phase silanization was carried out in anhydrous toluene that was dried and deoxygenated using a Pure Solv 400-6 solvent purification system (Innovative Technology). All glassware was cleaned in a base bath (potassium hydroxide in isopropyl alcohol and water), rinsed with distilled water (3×), and stored in a clean oven at 110 °C until use.
Publication 2011
3-(triethoxysilyl)propylamine 3-aminopropyltrimethoxysilane Bath Distillation Filtration Ion Exchange Isopropyl Alcohol Nitrogen Osmosis potassium hydroxide Silanes Silicon Solvents Toluene

Most recents protocols related to «3-(triethoxysilyl)propylamine»

Example 1

A nitrogen-purged autoclave reactor was charged with cyclohexane, tetrahydrofuran, styrene, and 1,3-butadiene. The temperature of the contents of the reactor was adjusted to 20° C., and then n-butyllithium was added to initiate polymerization. The polymerization was carried out under adiabatic conditions, and the maximum temperature reached 85° C. Once the polymerization conversion ratio reached 99%, 1,3-butadiene was further added, followed by polymerization for five minutes. Subsequently, N,N-bis(trimethylsilyl)-3-aminopropyltriethoxysilane was added as a modifier, and a reaction was performed. After completion of the polymerization reaction, 2,6-di-tert-butyl-p-cresol was added. Thereafter, the solvent was removed by steam stripping. The product was dried on hot rolls adjusted at 110° C. to obtain SBR 1.

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Patent 2024
1,3-butadiene 3-(triethoxysilyl)propylamine butyllithium cresol Cyclohexane Nitrogen Polymerization Rubber Solvents Steam Styrene TERT protein, human tetrahydrofuran
A PEG-biotin–coated glass chamber was made by silane coupling and a succinimidyl ester reaction. Cover glasses (C022221S; Matsunami Glass) were first subjected to sonication in 1 N KOH and plasma treatment. Glass surfaces were coated with an amino group by sandwiching N-2-(aminoethyl)-3-aminopropyl-triethoxysilane (KBE-603; Shin-Etsu Chemical) and incubation for 20 min at room temperature. These glasses were washed with deionized water 20 times and then incubated with 200 mg/ml of 0.5% biotinylated NHS-PEG (ME-050-TS and BI-050-TS; NOF) for 90 min at room temperature. Coated glasses were stuck to each other with 30-μm double-sided tape (5603; Nitto-Denko) to make an ∼2-mm wide flowing chamber. The glasses were sealed in a food saver in vacuo and stored at −80°C until use.
GMPCPP-bound tubulin labeled with Alexa Fluor 488 (2% labeled) and biotin (5% labeled) was incubated at 27°C for 1 h to promote nucleation, followed by 1 h of polymerization at 37°C. Polymerized microtubule samples were purified by centrifugation at 20,000g, 35°C for 25 min.
The glass chamber was treated as follows: first, 1 mg/ml NeutrAvidin was loaded into the chamber and incubated for 2 min to let it bind to biotin molecules covalently connected to the glass surface. The glass surface was subsequently deactivated by adding and incubating with 10× blocking solution (1 mg/ml casein and 1% pluronic F127) for 2 min. After washing them out with BRB80 containing 25% glycerol, GMPCPP-MT seeds were loaded into the chamber and incubated for 2 min to immobilize microtubules on the glass surface. Unbound microtubules were washed out with BRB80 containing 10x blocking solution and 1 mM GTP. To polymerize GDP-MT from the seeds, 28 μM Cy5-labeled tubulin (labeling rate 2%) in polymerization buffer (BRB80, 2× blocking solution, 1 mM GTP) was applied to the chamber, polymerized for 5 min, and terminated by washing free tubulin out with washout buffer (BRB80, 25% glycerol, and 2× blocking solution). To completely hydrolyze GTP, the chamber was incubated for more than 30 min before any subsequent experiments.
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Publication 2023
3-(triethoxysilyl)propylamine 5'-guanylylmethylenebisphosphonate alexa fluor 488 Biotin Buffers Caseins Centrifugation Esters Eyeglasses Food Glycerin Immobilization Microtubules neutravidin Plant Embryos Plasma Pluronic F-127 Silanes Tubulin
Nanofibrous and planar fibrinogen scaffolds
were prepared by our previously described salt-induced self-assembly
approach.43 (link),44 (link) Briefly, 100% clottable fibrinogen from
human plasma (Merck, Darmstadt, Germany), dissolved in 10 mM NH4HCO3 (Carl Roth GmbH, Karlsruhe, Germany) in deionized
H2O, was dialyzed overnight against the same solution using
a cellulose membrane dialysis tube (cutoff 14 kDa; Sigma-Aldrich,
Darmstadt, Germany) to obtain a fibrinogen solution. Fifteen millimeter
glass coverslips (VWR, Darmstadt, Germany) were cleaned with piranha
solution (3:1 of 95% sulfuric acid (H2SO4)/30%
hydrogen peroxide (H2O2)) before treatment with
5% (3-aminopropyl)triethoxysilane (APTES, Sigma) in ethanol (C2H5OH) overnight. Unbound APTES was removed by washing
in pure ethanol three times for 5 min, before storing the coverslips
dry for further use.
To obtain fibrinogen nanofibers, 5 mg mL–1 of the fibrinogen solution was dried in the presence
of 2.5× phosphate-buffered saline (PBS, ThermoFisher, pH 7.4)
on APTES-modified coverslips. Planar fibrinogen scaffolds were obtained
by drying 5 mg mL–1 of fibrinogen in 5 mM NH4HCO3. Drying was performed in a custom-built climate
chamber at a relative humidity of 30% and temperature of 24 °C for 12 h.
To maintain the stability
of scaffolds in an aqueous environment
for further cell culture studies, the scaffolds were cross-linked
in formaldehyde (FA) vapor for 2 h after placing them in a sealed
beaker. The FA vapor was generated by placing 1 μL of 37% FA
solution (Applichem GmbH, Darmstadt, Germany) per cubic centimeter
and letting it evaporate in the sealed beaker. After cross-linking
and devaporizing for another 30 min, all samples were washed 3 × 15 min with deionized water. We have extensively
characterized the nanofibrous topography with dense, porous nanofiber
networks of these fibrinogen nanofiber scaffolds as well as the smooth
topography of the planar fibrinogen scaffolds previously.43 (link),45 (link)To obtain un-cross-linked adsorbed fibrinogen substrates,
the so-called
physisorbed fibrinogen scaffolds, a slightly modified protocol from
a previously published method was used.46 (link) Fifteen millimeter glass coverslips (VWR) were cleaned with piranha
solution (3:1 of 95% sulfuric acid (H2SO4)/30%
hydrogen peroxide (H2O2)). A 0.1 mg mL–1 fibrinogen solution in PBS (Thermo Fisher, pH 7.4) was then dried
on the piranha-cleaned coverslips at 4 °C overnight. Subsequently,
the scaffolds were washed three times for 5 min with PBS. The physisorbed
fibrinogen scaffolds displayed a flat and smooth topography (see Supporting Information, Figure S4), also described
earlier.46 (link)Nanofibrous, planar, as
well as physisorbed fibrinogen scaffolds
were placed in wells of nontreated Corning Costar 24-well plates (Sigma)
and sterilized for 30 min using the UV light of a laminar flow cabinet
(ESI Flufrance) to further use them in cell culture experiments.
Publication 2023
3-(triethoxysilyl)propylamine Cell Culture Techniques Cellulose Cuboid Bone Dialysis Solutions Ethanol Fibrinogen Formaldehyde Humidity Peroxide, Hydrogen Phosphates Piranhas Plasma Saline Solution Scaffold Cell Culture Techniques Sodium Chloride sulfuric acid Tissue, Membrane Ultraviolet Rays
Prior to synthesizing
the monolithic packing, the polyimide-coated fused-silica capillaries
(100 μm i.d., 375 μm o.d.) from Polymicro Technologies
(Phoenix, AZ, USA) were first treated by an adapted etching protocol28 (link) at room temperature comprising a rinse with
methanol followed by deionized water, then etching with aqueous 1
M NaOH for 2 h followed by a rinse with deionized water until neutral.
This was followed by flushing with 1 M aqueous HCl for 20 min, deionized
water until neutral, methanol for 1 h, and drying by a flow of nitrogen
at room temperature for 12 h. These etched capillaries were subsequently
modified to produce three different activated capillaries (Figure S1; prefix “S” refers to
the Supporting Information), first by 10% (v/v) (3-aminopropyl)triethoxysilane
(APTES) in toluene at 60 °C overnight, followed by washing with
water and methanol and drying as in the final step of the above etching
procedure, yielding capillaries C1. Dried C1 capillaries
were subsequently reacted according to two different schemes; capillaries C2 were prepared by filling C1 with 10% (v/v) aqueous formaldehyde
and reacting at 80 °C for 4 h, followed by washing and drying
as above; capillaries C3 were filled with the MF precondensate
(see below) diluted 1 + 9 (v/v) with water and reacted at 80 °C
for 4 h, followed by washing and drying as above. Activated capillaries
were stored in a desiccator for use in the polymerization of MF monolithic
columns.
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Publication 2023
3-(triethoxysilyl)propylamine Capillaries Methanol Polymerization Silicon Dioxide Toluene
For Paper_AuNPs_CIN, 1 mL of 10 mM HAuCl4 (99.9%, MERCK) ethanol solution was poured over a sheet of Whatman
#1 filter paper (47 mm in diameter) and dried in a convection oven
at 60 °C for 1 h (Scheme 1). The paper containing Au ions was then immersed in 5 mL
of a 10 mM sodium borohydride (NaBH4, MERCK) aqueous solution
for an additional hour. Au ions settled on the filter paper were reduced
to AuNPs via a reduction reaction with NaBH4, resulting
in an instantaneous color change from white to purple. The paper containing
the AuNPs, labeled Paper_AuNPs, was then rinsed with deionized water
and ethanol to remove any residual reagents and dried in a convection
oven at 60 °C. The Paper_AuNPs film was submerged in 10 mL of
toluene containing 5% 3-aminopropyltriethoxysilane (APTES, 99%, MERCK)
and placed in a 30 °C, 100 rpm, shaking incubator for 1 day.
The paper was then rinsed with toluene and dried in a convection oven
to remove any residual APTES; it was then labeled as Paper_Si_AuNPs.
The Paper_Si_AuNPs were then immersed in 10 mL of ethanol containing
5% trans-cinnamaldehyde (CIN, 99%, MERCK) and placed
in a 50 °C, 100 rpm incubator for 1 day. The Paper_AuNPs_CIN
film was fabricated by connecting amine groups on the outer surface
of the Paper_Si_AuNPs film to trans-cinnamaldehyde
(CIN, 99%, MERCK) via an imine (C=N) bond. The fabrication of Paper_AuNPs_CIN
was completed by a drying procedure following three 5 min ultrasonication
treatments with ethanol. The paper CIN was produced using the identical
method without AuNPs reduction (Scheme 2).
Publication 2023
3-(triethoxysilyl)propylamine Amines cinnamic aldehyde Convection Ethanol gold tetrachloride, acid Imines Ions sodium borohydride Toluene

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3-aminopropyltriethoxysilane is a bifunctional organosilane compound. It contains both an amino group and three ethoxy groups. This molecule can be used as a coupling agent in various applications, facilitating the bonding between inorganic and organic materials.
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3-aminopropyltriethoxysilane (APTES) is a silane coupling agent that can be used for modifying the surface properties of various materials, such as glass, metals, and polymers. It contains a primary amine group and three ethoxy groups, which allow for covalent attachment to substrates. APTES is commonly used in applications involving surface functionalization, adhesion promotion, and biomolecule immobilization.
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APTES is a silane-based chemical compound that is commonly used as a coupling agent in various applications. It is a colorless, viscous liquid with the chemical formula (CH3CH2O)3Si(CH2)3NH2. APTES is known for its ability to form covalent bonds between inorganic materials, such as glass or metal, and organic molecules, making it a valuable tool in surface modification and adhesion enhancement.
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Tetraethyl orthosilicate is a chemical compound used in the manufacturing of various laboratory equipment and materials. It is a clear, colorless liquid with a specific chemical formula of Si(OC2H5)4. The primary function of tetraethyl orthosilicate is to serve as a precursor for the synthesis of silicon-based materials, including silica gels, glasses, and coatings.
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.

More about "3-(triethoxysilyl)propylamine"

3-(triethoxysilyl)propylamine is a silane-based chemical compound with a range of applications in surface modification, polymer synthesis, and materials science.
It is also known as 3-aminopropyltriethoxysilane (APTES) and can be used to introduce functional groups onto surfaces or into polymeric materials.
Researchers optimizing their 3-(triethoxysilyl)propylamine research can utilize PubCompare.ai's AI-driven reproducibility platform to easily locate protocols from literature, preprints, and patents.
The platform's AI-driven comparisons can help identify the best protocols and products for their experiments, streamlining the research process and ensuring reliable results.
APTES is often used in conjunction with other silane-based reagents, such as tetraethyl orthosilicate (TEOS), to create functionalized surfaces or to modify the properties of polymeric materials.
It can also be used in combination with compounds like hydrochloric acid, sodium hydroxide, and ethanol for various surface treatment and modification applications.
In addition to surface modification, 3-(triethoxysilyl)propylamine has applications in the preparation of bioactive materials, such as those involving bovine serum albumin (BSA) and N-hydroxysuccinimide (NHS).
These materials can be used in a variety of biomedical and biotechnological applications, including drug delivery, tissue engineering, and biosensing.
Researchers can streamline their 3-(triethoxysilyl)propylamine research and achieve reliable results by utilizing PubCompare.ai's AI-driven reproducibility platform, which helps locate the best protocols and products for their experiments.
This can be particularly useful when working with APTES and related silane-based compounds, as well as in the development of bioactive materials involving DMSO and other solvents.