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Alabaster

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Most cited protocols related to «Alabaster»

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Publication 2008
1,2-dihexadecyl-sn-glycero-3-phosphocholine Alabaster austin Brain Stem Buffers Cells Cerebellum Chloroform Cholinergic Agents Cold Temperature Cycloheximide Deoxyribonucleases Digestion Dithiothreitol Endoribonucleases Ethanol G-substrate Goat HEPES inhibitors Isopropyl Alcohol Lipids Magnesium Chloride Mice, Laboratory Mice, Transgenic Motor Neurons Nonidet P-40 Polyribosomes Protease Inhibitors Purkinje Cells Ribosomal RNA RNA, Messenger Sodium Acetate Sodium Chloride Striatum, Corpus Teflon Tissues trizol
Lipid-polymer hybrid NPs were prepared via self-assembly of PLGA (poly (D,L-lactic-co-glycolic acid); Lactel, Pelham, AL), lecithin (soybean, refined, molecular weight: ~330 Da; Alfa Aesar, Ward Hill, MA), and DSPE-PEG (1,2-distearoyl-sn-glycero-3-phosphoethanolamine- N-carboxy (polyethylene glycol)2000); Avanti, Alabaster, AL) through a single-step nanoprecipitation method. Briefly, PLGA polymer was dissolved in acetonitrile with concentrations ranging from 1~5 mg/mL. Lecithin/DSPE-PEG (8.5/1.5, molar ratio) with a weight ratio of 15% to the PLGA polymer were dissolved in 4 wt% ethanol aqueous solution. The lecithin/DSPE-PEG solution was heated to 65°C to ensure all lipids were in liquid phase. The resulting PLGA solution was then added into the preheated lipid solution dropwise under gentle stirring. The mixed solution was vortexed vigorously for 3 minutes followed by gentle stirring for 2 hours at room temperature. The remaining organic solvent and free molecules were removed by washing the NP solution three times using an Amicon Ultra-4 centrifugal filter (Millipore, Billerica, MA) with a molecular weight cut-off of 10,000 Da. To prepare drug-encapsulated NPs, docetaxel (Sigma-Aldrich, St Louis, MO) with proper initial dosage was dissolved into the PLGA acetonitrile solution before the nanoprecipitation process. NP size (diameter, nm) and surface charge (zeta potential, mV) were obtained from three repeat measurements by Quasi-elastic laser light scattering with a ZetaPALS dynamic light scattering detector (15 mW laser, incident beam = 676 nm; Brookhaven Instruments Corporation, Holtsville, NY).
Publication 2008
1,2-distearoylphosphatidylethanolamine acetonitrile Alabaster DA10 Docetaxel Ethanol glycolic acid Hybrids Lecithin Lipids Molar Pharmaceutical Preparations Phosphatidylethanolamines Poly A polyethylene glycol 2000 Polylactic Acid-Polyglycolic Acid Copolymer Polymers Solvents Soybeans

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Publication 2012
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy-poly(ethylene glycol 2000) 1,2-distearoyllecithin Alabaster Excipients Fluorocarbons Glycerin Lipid A Lipids Microbubbles Molar monomethoxypolyethylene glycol perfluorobutane perflutren Phosphates Phosphatidylethanolamines Propylene Glycol Saline Solution Syringes
E. coli total lipid extract was purchased from Avanti Polar Lipids (Alabaster, AL, USA) and analyzed on the LTQ Orbitrap XL instrument in negative ion mode. A solution of the total lipid concentration of 2.5 μg/ml in 7.5 mM ammonium acetate in choloroform/methanol/2-propanol (1/2/4, v/v/v) was infused into the mass spectrometer by TriVersa robotic ion source using a chip with the diameter of spraying nozzles of 4.1 μm. To produce the spectra dataset, the extract was analyzed in several independent experiments: experiment I, eight acquisitions under the unit mass resolution (R) settings using ion trap (IT) to acquire both MS and MS/MS spectra; experiment II, six acquisitions with R = 7,500 for MS spectra (Orbitrap) and unit resolution for MS/MS spectra (IT); experiment III, four acquisitions with R = 30,000 for MS spectra (Orbitrap) and unit resolution for MS/MS spectra (IT); experiment IV, four acquisitions with R = 100,000 for MS spectra (Orbitrap) and unit resolution for MS/MS spectra (IT); experiment V, seven acquisitions with R = 100,000 for MS spectra (Orbitrap) and R = 15,000 for MS/MS spectra (Orbitrap).
In the experiments I to IV, each acquisition produced approximately 33 MS and 330 MS/MS spectra; in the experiment V, 10 MS and 100 MS/MS spectra were acquired. To reduce undersampling, in the experiment V, acquisition of MS/MS spectra was navigated by the inclusion list compiled from 40 masses of plausible PE, PG and PA precursors A list of molecular lipid species was produced by manual interpretation of spectra acquired in the experiment V with requested mass tolerance of better than 3 ppm for precursors and 5 ppm for specific fragment ions. Only lipid species identified in at least four out of seven replicated analyses were included.
Spectra acquired in each of the experiments I to IV were further processed by LipidXplorer to produce corresponding MasterScan files. We used the dataset from the experiment I for comparative benchmarking of LipidXplorer against LipidQA and LipidSearch programs. Since LipidQA and LipidSearch do not align the spectra from replicated analyses, each acquisition was processed independently and then a non-redundant list of all identified lipid species was compiled.
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Publication 2011
1-Propanol Alabaster ammonium acetate DNA Chips Escherichia coli Immune Tolerance Ions Lipid A Lipids Methanol Spectrum Analysis Tandem Mass Spectrometry
Proteoliposomes for content-mixing assays were prepared by detergent dialysis in RB150/Mg2+ (20 mM HEPES-NaOH, pH 7.4, 150 mM NaCl, 1 mM MgCl2, 10% glycerol [vol/vol]) as described (Zucchi and Zick, 2011 (link)) from lipid mixes mimicking the vacuolar composition (43.6 mol% 1,2-dilinoleoyl-sn-glycero-3-phosphocholine, 18 mol% 1,2-dilinoleoyl-sn-glycero-3-phosphoethanolamine, 18 mol% soy l-α-phosphatidylinositol, 4.4 mol% 1,2-dilinoleoyl-sn-glycero-3-phospho-l-serine, 2 mol% 1,2-dilinoleoyl-sn-glycero-3-phosphate, 1 mol% 16:0 1,2-dipalmitoyl-sn-glycerol [all from Avanti Polar Lipids, Alabaster, AL]; 8 mol% ergosterol [Sigma-Aldrich, St. Louis, MO], 1 mol% each of di-C16 phosphatidylinositol 3-phosphate and phosphatidylinositol 4,5-bisphosphate [Echelon Biosciences] and 3 mol% 7-nitrobenz-2-oxa-1,3-diazole [NBD]–1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine [DPPE; Life Technologies, Carlsbad, CA]) for donor liposomes or 3 mol% Marina-Blue-DPPE for acceptor, subsets of the four vacuolar SNAREs, and Ypt7p, entrapping Cy5-labeled streptavidin or biotinylated R-phycoerythrin. Molar protein:lipid ratio was 1:2500 for SNAREs and 1:2000 for Ypt7p. Isolation after reconstitution was achieved by floatation on a three-step Histodenz gradient (35, 25% Histodenz [wt/vol] and RB150/Mg2+). Histodenz (Sigma-Aldrich) solutions were prepared as 70% stock solution in modified RB150/Mg2+ with a reduced concentration (2% [vol/vol]) of glycerol to compensate for the osmotic activity of the density medium; lower-concentration solutions were obtained by dilution with RB150/Mg2+. RPLs for experiments based on lipid dequenching were prepared with 1.5 mol% of NBD-DPPE and rhodamine-DPPE for donor RPLs and without fluorescent lipids for acceptor RPLs. RPLs for experiments based on tethering via streptavidin were prepared with 0.1 mol% 18:1 Biotinyl Cap PE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) [Avanti Polar Lipids]) and without entrapped content markers.
Publication 2013
1,2-dipalmitoyl-3-phosphatidylethanolamine 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) Alabaster Biological Assay bis(diphenylphosphine)ethane Detergents Dialysis dioleoyl cephalin Ergosterol Glycerin Glycerylphosphorylcholine HEPES isolation Lipids Liposomes Magnesium Chloride Molar Osmosis Phosphates Phosphatidylethanolamines phosphatidylinositol 3-phosphate Phosphatidylinositols Phycoerythrin Proteins proteoliposomes RB150 Rhodamine Serine SNAP Receptor Sodium Chloride Streptavidin Technique, Dilution Tissue Donors Vacuole

Most recents protocols related to «Alabaster»

Stock solutions (1–10 mg/mL)
of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
(POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine (POPS, Avanti Polar Lipids, Alabaster, AL, USA), and
ATTO 390-1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine
(Atto 390-DPPE, ATTO-TEC, Siegen, Germany) were prepared in chloroform. l-α-Phosphatidylinositol-4,5-bisphosphate (PtdIns[4,5]P2, brain porcine, Avanti Polar Lipids, Alabaster, AL, USA)
was freshly dissolved in chloroform/methanol/H2O (10:20:8)
to a final concentration of 1 mg/mL. Lipid mixtures (0.4 mg) were
prepared in chloroform/methanol (10:1), and organic solvents were
evaporated with a nitrogen stream followed by 3 h in vacuum. The dried
lipid films were stored at 4 °C until needed.
Small unilamellar
vesicles (SUVs) were prepared by rehydrating a lipid film in spreading
buffer (50 mM KCl, 20 mM Na-citrate, 0.1 mM NaN3, 0.1 mM
ethylenediaminetetraacetic acid (EDTA), pH 4.8),38 (link) incubating for 30 min, subsequent vortexing (3 × 30
s at 5 min intervals), and a final sonification step for 30 min at
room temperature (cycle 4, 60%, Sonopuls HD2070, resonator cup; Bandelin,
Berlin, Germany). PtdIns[4,5]P2 containing SUVs were used
immediately for the preparation of SLBs to avoid PtdIns[4,5]P2 degradation.65 (link)
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Publication 2023
1-palmitoyl-2-oleoylphosphatidylcholine Acids Alabaster bis(diphenylphosphine)ethane Brain Chloroform Citrates Edetic Acid Lipid A Lipids Methanol Nitrogen Phosphatidylethanolamines Phosphatidylinositols Pigs Serine Sodium Azide Solvents Vacuum
Extraction of liver lipids was performed according to the method described by Folch et al.30 (link). Dihydroceramides and ceramides and were isolated by solid phase extraction chromatography using C12:0 dihydroceramide and C17:0 ceramide (Avanti Polar Lipids, Alabaster, Al, USA) as internal standards. Samples were analysed by liquid chromatography-mass spectrometry (LC–MS) using a Thermo Exactive Orbitrap mass spectrometer (Thermo Scientific, Hemel Hempsted, UK) equipped with a heated electrospray ionization (HESI) probe and coupled to a Thermo Accela 1250 ultra-high-pressure liquid chromatography (UHPLC) system. Samples were injected onto a Thermo Hypersil Gold C18 column (2.1 mm by 100 mm; 1.9 μm) maintained at 50 °C. Mobile phase A consisted of water containing 10 mM ammonium formate and 0.1% (vol/vol) formic acid. Mobile phase B consisted of a 90:10 mixture of isopropanol-acetonitrile containing 10 mM ammonium formate and 0.1% (vol/vol) formic acid. The initial conditions for analysis were 65% mobile phase A, 35% mobile phase B and the percentage of mobile phase B was increased from 35 to 65% over 4 min, followed by 65% to 100% over 15 min, with a hold for 2 min before reequilibration to the starting conditions over 6 min. The flow rate was 400 μl/min and samples were analyzed in positive ion mode. The LC–MS data were processed with Thermo Xcalibur v2.1 (Thermo Scientific) with signals corresponding to the accurate mass-to-charge ratio (m/z) values for dihydroceramide and ceramide molecular species extracted from raw data sets with the mass error set to 5 ppm. Quantification was achieved by relating the peak area of the dihydroceramide and ceramide lipid species to the peak area of their respective internal standard. All values were normalised to the wet weight of liver.
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Publication 2023
acetonitrile Alabaster Altretamine Ceramides Chromatography dihydroceramide formic acid formic acid, ammonium salt Gold High-Performance Liquid Chromatographies Isopropyl Alcohol Lipids Liquid Chromatography Liver Mass Spectrometry Solid Phase Extraction
Protein kinase A, lactate dehydrogenase, and phosphoenol-pyruvate were purchased from Roche CustomBiotech (Indianapolis, IN). Adenosine-5′-triphosphate disodium salt (ATP) ultrapure 98% was obtained from Alfa Aesar (Tewksbury, MA). Verapamil was acquired from Sigma Aldrich (Saint Louis, MO). n-dodecyl-β-D-maltopyranoside (DDM) was bought from Inalco S. p.A (Milano, Italy). Nicotinamide adenine dinucleotide (NADH) was purchased from Sigma-Aldrich (Burlington, MA).
E. coli polar lipids (polar extract) and synthetic lipids were acquired from Avanti (Alabaster, AL); these include 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine or 16:0-18:1 PC (POPC), 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylinositol (POPI), 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylglycerol (POPG), DPPA, 1,2-dipalmitoyl-sn-glycero-3-phosphate or 16:0 PA, 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC). Sphingomyelin (SM) was >99% pure from porcine brain with major acyl chains of 18:0 (50%) and 21:1 (21%), and cardiolipin (CL) was from >99% bovine heart with major acyl chains of 18:2 (90%). All synthetic lipids, SM and CL had very low tryptophan fluorescence (ex/em 295/350 nm) if purchased as powder. Cholesterol (Chol) and cholesteryl hemisuccinate (CHS) were purchased from Anatrace (Maumee, OH).
General chemicals were at the highest grade from Thermo Fisher Scientific (Waltham, Massachusetts).
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Publication 2023
1-palmitoyl-2-oleoylphosphatidylcholine Adenosine Triphosphate Alabaster Brain Cardiolipins Cattle Cholesterol cholesterol-hemisuccinate Coenzyme I Cyclic AMP-Dependent Protein Kinases Dimyristoylphosphatidylcholine Escherichia coli Fluorescence Glycerylphosphorylcholine Heart Lactate Dehydrogenase Lipids Phosphates Phosphatidylethanolamines Phosphatidylglycerols Phosphatidylinositols Phosphoenolpyruvate Pigs Powder Serine Sodium Chloride Sphingomyelins Tryptophan Verapamil
Cholesterol, distearoyl-sn-glycero-3-phosphocholine (DSPC), and dimyristoyl-rac-glycero-3-methoxypolyethylene glycol (PEG-DMG) were from Avanti Polar Lipids (Alabaster, AL, USA). The proprietary ionizable lipid (lipid 14) was synthesized in-house by G. S. Naidu (fig. S3) (48 (link)). mRNA sequences were purchased from TriLink (San Diego, CA, USA) or synthesized by an in vitro transcription (IVT) reaction using the MEGAscript T7 transcription kit and cleaned by the MEGAclear transcription clean-up kit both from Thermo Fisher Scientific (Waltham, MA, USA). All mRNA sequences were synthesized with complete N1-methyl-pseudouridine nucleotide substitution.
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Publication 2023
1,2-distearoyllecithin 1-methylpseudouridine ACTR protein, human Alabaster Cholesterol Lipids monomethoxypolyethylene glycol Nucleotides RNA, Messenger Transcription, Genetic
The phospholipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-hexadecanoyl-2-(9-Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (POPG), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine (POPS), and Liss-Rhod-DOPE were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Cholesterol was obtained from Sigma Aldrich (Shanghai, China). Sodium chloride, chloroform, 4-hydroxyethyl piperazine sulfonic acid (HEPES), hydrochloric acid, sodium hydroxide and propranolol hydrochloride were of analytical grade and were purchased from Titan (Shanghai, China). Microscope coverslips (22 × 40 mm, no. 1.5) was supplied by Fisher Scientific (Pittsburgh, Pennsylvania, USA). PDMS (Dow Corning Sylgard Silicone Elastomer-184) was provided from Krayden, Inc. (El Paso, TX). Purified water (18.25 mΩ cm) was produced from a Direct-pure UP Water System (RephiLe Bioscience, Ltd, China).
Publication 2023
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine Alabaster Chloroform Cholesterol Glycerin Glycerylphosphorylcholine Hydrochloric acid Lipids Microscopy Phosphatidylethanolamines Phospholipids Piperazine Propranolol Hydrochloride Silicone Elastomers Sodium Chloride Sodium Hydroxide Sulfonic Acids

Top products related to «Alabaster»

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Cholesterol is a lab equipment product that measures the concentration of cholesterol in a given sample. It provides quantitative analysis of total cholesterol, HDL cholesterol, and LDL cholesterol levels.
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Chloroform is a colorless, volatile liquid with a characteristic sweet odor. It is a commonly used solvent in a variety of laboratory applications, including extraction, purification, and sample preparation processes. Chloroform has a high density and is immiscible with water, making it a useful solvent for a range of organic compounds.
Sourced in United States
1,2-dioleoyl-sn-glycero-3-phosphocholine is a synthetic lipid compound. It is a phospholipid that consists of two oleic acid chains attached to a glycerol backbone, with a phosphocholine headgroup.
Sourced in United States, United Kingdom
1,2-dipalmitoyl-sn-glycero-3-phosphocholine is a synthetic phospholipid commonly used as a model system for the study of lipid membranes and their properties. It has a molecular formula of C40H80NO8P and is composed of two palmitoyl fatty acid chains attached to a glycerol backbone, with a phosphocholine head group.
Sourced in United States
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine is a phospholipid consisting of a glycerol backbone with a palmitic acid and an oleic acid esterified to the first and second carbons, respectively, and a phosphocholine group attached to the third carbon. This compound is a commonly used lipid in various biochemical and biophysical applications.
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Cholesterol is a lipid compound found in animal cells. It is a core component of cell membranes and is essential for various physiological processes. Avanti Polar Lipids offers high-purity cholesterol for use in research and laboratory applications.
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The Mini-extruder is a compact and versatile laboratory device designed for the extrusion of lipid vesicles and liposomes. It features a manual operation mechanism that allows for controlled and reproducible extrusion of samples through polycarbonate membranes with defined pore sizes.
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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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1,2-dimyristoyl-sn-glycero-3-phosphocholine is a synthetic phospholipid commonly used in research applications. It is a common component of cell membranes and can be used to model lipid bilayers.

More about "Alabaster"

Alabaster, the revolutionary AI-driven platform from PubCompare.ai, is transforming the way researchers approach research protocols.
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Integrating relevant concepts from related areas, Alabaster's functionality extends beyond just protocol discovery.
For instance, the platform's ability to handle data related to cholesterol, chloroform, phospholipids (such as 1,2-dioleoyl-sn-glycero-3-phosphocholine, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine), and even common laboratory techniques like mini-extruding and methanol-based procedures, further enhances its versatility and relevance across diverse research domains.
By combining the power of artificial intelligence with a comprehensive database of protocols, Alabaster revolutionizes the way researchers approach their work.
Whether you're investigating the properties of cholesterol, exploring the applications of chloroform, or optimizing your cell culture procedures using FBS (Fetal Bovine Serum) and phospholipids like 1,2-dimyristoyl-sn-glycero-3-phosphocholine, Alabaster's intuitive tools and AI-driven insights can streamline your research process and propel your discoveries forward.
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