HeLa cells were seeded in 24-well plates at a density of 7 × 104 cells/well and grown up to 60–70% confluence. Endotoxin-free plasmid DNA was prepared with anion-exchange resin columns (Qiagen) and diluted in sterile 10 mM Tris–HCl buffer. Cells were transfected with Lipofectamine 2000 reagent (Invitrogen) following the manufacturer’s protocol. Each well received 200 ng plasmid DNA in the single-vector assay, 0.6 µl Lipofectamine 2000 reagent and 100 µl GlutaMAX-I-supplemented Opti-MEM I reduced-serum medium (Invitrogen). In the two-vector assay, a 20:1 ratio of L1 plasmid (200 ng) and the Rluc control plasmid pGL4.73 (10 ng) was used. Puromycin (Sigma-Aldrich) was added in complete medium at a final concentration of 2.5 µg/ml at 24 h post-transfection. Cells were harvested 4 days post-transfection for luciferase activity analysis unless otherwise indicated. To perform single-vector assays in 96-well plates, HeLa cells were seeded at a density of 1 × 104 cells/well and grown up to 80–85% confluence. One-hundred-nanogram plasmid DNA, 0.3 µl Lipofectamine 2000 reagent and 50 µl GlutaMAX-I-supplemented Opti-MEM I reduced-serum medium were used per well.
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Anion Exchange Resins
Anion Exchange Resins
Anion Exchange Resins are a class of materials used in a wide range of scientific applications, including water treatment, ion chromatography, and biochemical purification.
These resins are designed to selectively bind and exchange anions, making them valuable tools for researchers studying ionic processes and developing new separation techniques.
PubCompare.ai's platform offers a powerful AI-driven comparison tool to help scientists optimize their Anion Exchange Resins research, easily locate the best protocols from literature, pre-prints, and patents, and leverage AI-powered insights to identify the most effective products and methodologies for their experiments.
Take your Anion Exchange Resins research to the next level with PubCompare.ai - your one-stop-shop for scientific discovery.
These resins are designed to selectively bind and exchange anions, making them valuable tools for researchers studying ionic processes and developing new separation techniques.
PubCompare.ai's platform offers a powerful AI-driven comparison tool to help scientists optimize their Anion Exchange Resins research, easily locate the best protocols from literature, pre-prints, and patents, and leverage AI-powered insights to identify the most effective products and methodologies for their experiments.
Take your Anion Exchange Resins research to the next level with PubCompare.ai - your one-stop-shop for scientific discovery.
Most cited protocols related to «Anion Exchange Resins»
Anion Exchange Resins
Biological Assay
Cells
Cloning Vectors
Endotoxins
HeLa Cells
lipofectamine 2000
Luciferases
Paragangliomas 4
Plasmids
Puromycin
Serum
Sterility, Reproductive
Transfection
Tromethamine
Total RNA was isolated using either phenol or anion exchange resin. Phenol extraction was carried out using a protocol adapted from Chomczynski and Sacchi [26 (link)]. In short, samples were disrupted (5% weight/volume) in lysis buffer (4 M guanidine thiocyanate, 25 mM pH7.0 sodium citrate, 0.5% N-laurylsarcosine and 0.1 M β-mercaptoethanol) with a blender (Waring Blender, New Hartford, USA). RNA isolation was carried out from 900 μL of lysate with 90 μL of 2 M pH4.0 sodium acetate, 810 μL of phenol and 180 μL of 24/1 chloroform / isoamylic alcohol. Conversely, total RNA was isolated from 30 mg samples, using RNeasy mini kit (QIAGEN S.A., Courtabeuf, France) following the manufacturer's instructions with the optional RNase-free DNase step to avoid contamination with genomic DNA.
2-Mercaptoethanol
Anion Exchange Resins
Buffers
Chloroform
Deoxyribonucleases
DNA Contamination
Endoribonucleases
Ethanol
Genome
guanidine thiocyanate
isolation
Phenols
Sodium Acetate
Sodium Citrate
The aptamers were transcribed in vitro from a linearized pSP64 plasmid (Promega) using a T7 promoter. For precise aptamer 3′ ends the primary transcripts contained self-cleaving hammerhead ribozymes (all sequences are available upon request). Run-off transcription was done in a volume of 2 ml containing 40 mM Tris-HCl (pH 8.0), 5 mM DTT, 1 mM spermidine, 30 mM Mg-acetate, 4 mM each NTP, 0.1 mg/ml HindIII-linearized plasmid and 15 µg/ml T7 RNA polymerase [prepared according (14 (link))]. Reactions were incubated at 37°C for 4 h, subsequently diluted 1:5 in 30 mM Mg-acetate and subjected to two annealing cycles (5 min at 65°C, followed by slow-cooling to room temperature over 45 min) in order to maximize self cleavage of the ribozyme. After dissolving precipitated Mg-pyrophosphate by dropwise addition of 0.5 M EDTA (pH 8.0), the reaction products were pre-purified on 1 ml pre-equilibrated DEAE–Sepharose FF anion exchange resin [Pharmacia®, 0.7 cm column diameter, equilibration in 300 mM, elution in 3 M Na-acetate (pH 7.5)]. After ethanol-precipitation, the RNA products were separated on a 10% denaturing polyacrylamide gel. Aptamer RNA was detected by ultraviolet (UV) shadowing and eluted from crushed gel slices in 300 mM Na-acetate over night at room temperature. The supernatant was again concentrated on DEAE–Sepharose and ethanol precipitated. RNA was resuspended in H2O (0.1–10 mg/ml) and stored frozen at −20°C. Prior to complex formation the RNA was adjusted to the respective buffer system and annealed (see above). Complexation with tc was assured by an additional co-incubation for 5 min at 37°C.
2-diethylaminoethanol
Acetate
Anion Exchange Resins
bacteriophage T7 RNA polymerase
Buffers
Catalytic RNA
Cytokinesis
Edetic Acid
Ethanol
Freezing
hammerhead ribozyme
magnesium pyrophosphate
Plasmids
polyacrylamide gels
Promega
Sepharose
Spermidine
Transcription, Genetic
Tromethamine
Commercially available anion exchange resin membranes (551642S, VWR) were cut in 2 × 3 cm pieces. Membranes were placed in 0.5 M HNO3 for 1 h for cleaning, rinsed with sub-boiled water, and regenerated for 4 h in a 0.5 M NaHCO3 (p.a., Sigma-Aldrich) solution. The regenerated membranes were rinsed with sub-boiled water and placed into 15 mL of standard solution or sample. These solutions (containing the membranes) were shaken for 16 h. The membranes were rinsed with sub-boiled water, and the adsorbed sulfate was extracted from the membrane in 15 mL 2 % HNO3 within 1 h of shaking.
Recovery of sulfate was tested for the SO42− concentration range found in our soil solution samples. The recovery was tested for actual samples, as well. Since other anions can be found in the soil solution in significant amounts, the influence of anion competition on SO42− exchange on the membrane was investigated: Cl− and NO3− anions in concentrations of 0.1, 1.0, 5.0, 10, and 15 mmol L−1 were added to the sulfate standard (0.6 mmol L−1 SO42-). The kinetics of anion exchange on the membrane were investigated by placing regenerated resin membranes into a standard solution (0.9 mmol L−1 SO42−) for 10 min, 30 min, and 1, 2, 4, 8, and 16 h. In order to test for sulfate preconcentration by the anion exchange membrane, we reduced the volume of the elution solution to 10 mL and to 5 mL 2 % (m/m) HNO3.
Recovery of sulfate was tested for the SO42− concentration range found in our soil solution samples. The recovery was tested for actual samples, as well. Since other anions can be found in the soil solution in significant amounts, the influence of anion competition on SO42− exchange on the membrane was investigated: Cl− and NO3− anions in concentrations of 0.1, 1.0, 5.0, 10, and 15 mmol L−1 were added to the sulfate standard (0.6 mmol L−1 SO42-). The kinetics of anion exchange on the membrane were investigated by placing regenerated resin membranes into a standard solution (0.9 mmol L−1 SO42−) for 10 min, 30 min, and 1, 2, 4, 8, and 16 h. In order to test for sulfate preconcentration by the anion exchange membrane, we reduced the volume of the elution solution to 10 mL and to 5 mL 2 % (m/m) HNO3.
Anion Exchange Resins
Anions
Bicarbonate, Sodium
CM 2-3
Kinetics
Resins, Plant
Sulfates, Inorganic
Tissue, Membrane
Collagen: To remove the extractable collagen pool, 1 ml of 1 mg/ml α-chymotrypsin (TLCK Treated, type VII: from bovine pancreas, Sigma) in 50 mM Tris, pH 7.6, containing proteinase inhibitors (1 mM EDTA, 1 mM iodoacetamide and 10 μg/ml pepstatin-A) was added to 50 mg of diced cartilage sample. After incubation at 37°C overnight the supernatant was removed. The supernatant and the saline, used for equilibration, were diluted quantitatively 1:1 with 12 N HCl and the residue was immersed in 6 N HCl to be hydrolyzed at 110°C overnight. The hydrolysates were then dried at 90°C, reconstituted with 0.5 ml distilled water and dried again to remove traces of HCl. Finally, samples were dissolved in 0.5 ml distilled water and clarified by adding charcoal resin decolorizer (prepared from equal amounts of activated charcoal and AG-1 X8 anion exchange resin). The amount of hydroxyproline (μg/mg tissue) was measured by colorimetric methodology at 550 nm using L-4- hydroxyproline (Fluka) as a standard
[22 (link)].
Results are reported as μg hydroxyproline, while in the discussion we refer to collagen content. The extractable collagen pool is expressed as percentage of the total collagen amount, as previously reported
[23 (link)].
GAG: To another 10 mg of diced cartilage sample 1 μl of 19 mg/ml papain (papaya latex, EC 3.4.22.2, Sigma) in 0.1 M Tris–HCl, pH 7.2, containing 10 mM disodium EDTA and 5 mM cysteine-HCl, was added. After incubation at 60°C for 18 hours the digest was removed
[24 (link)]. The amount of GAG was determined by a commercially available colorimetric kit using dye-precipitation of sulphated GAGs with Alcian blue
[25 (link)].
[22 (link)].
Results are reported as μg hydroxyproline, while in the discussion we refer to collagen content. The extractable collagen pool is expressed as percentage of the total collagen amount, as previously reported
[23 (link)].
GAG: To another 10 mg of diced cartilage sample 1 μl of 19 mg/ml papain (papaya latex, EC 3.4.22.2, Sigma) in 0.1 M Tris–HCl, pH 7.2, containing 10 mM disodium EDTA and 5 mM cysteine-HCl, was added. After incubation at 60°C for 18 hours the digest was removed
[24 (link)]. The amount of GAG was determined by a commercially available colorimetric kit using dye-precipitation of sulphated GAGs with Alcian blue
[25 (link)].
Alcian Blue
Anion Exchange Resins
Bos taurus
Carica papaya
Cartilage
Charcoal
Charcoal, Activated
Chymotrypsin
Collagen
Colorimetry
Cysteine Hydrochloride
Edetic Acid
EDTA, Disodium
Hydroxyproline
Iodoacetamide
Latex
Pancreas
Papain
pepstatin
Protease Inhibitors
Resins, Plant
Saline Solution
Tissues
Tosyllysine Chloromethyl Ketone
Tromethamine
Most recents protocols related to «Anion Exchange Resins»
The XOS contents were evaluated by high-performance anion-exchange chromatography (HPAEC). Samples were dissolved in ultrapure water, oscillated by ultrasound, and then centrifuged at 6000 × g for 15 min, and 1 mL of the supernatant was collected. Standards of xylobiose (X2), xylotriose (X3), xylotetraose (X4), xylopentaose (X5), and xylohexaose (X6) were purchased from Megazyme (Wicklow, Ireland, UK). The following steps were conducted according to the method [18 (link)]. Analysis of the standards and filtered samples was carried out on a Dionex ICS3000 system equipped with a pump and an amperometric detector. The chameleon chromatography management system (Dionex, Sunnyvale, CA, USA) was used for sugar identification and quantification. An analytical CarboPac PA10 pellicular anion-exchange resin column (250 mm × 4 mm) was used for sugar separation. The monoses were eluted with 250 mmol/L NaOH at a flow rate of 1.0 mL/min.
Anion Exchange Resins
Anions
Carbohydrates
Chameleons
Chromatography
Dental Pellicle
Ultrasonics
xylobiose
xylotriose
Fresh Rhizomes of Rehmannia glutinosa were cut into small pieces of 5–10 mm after being washed, added four times the amount of water, and extracted twice at 90°C, at 1 h duration. The two extracts were combined, adding activated carbon (2 g/100 ml) and activated clay (2 g/100 ml) to the extract. It was stirred and decolored at 80°C for 30 min, then centrifuged. The supernatant was passed through 001 × 7 cation exchange resin column (diameter: high = 6:1), D201 type anion exchange resin column (diameter: high = 6:1), D101 macroporous adsorption resin column (diameter: high = 10:1) one by one, sample volume (mL): resin column volume = 1:1.5, flow rate was 500 mL/h. Finally, the macroporous adsorption resin effluent was collected and concentrated and dried at 60°C to get white powder, that is RGO.
The type and content of oligosaccharides in RGO were detected using high-performance liquid chromatography (HPLC) (Agilent1260), configured using a Refractive Index Detector (RID) (13 (link)). The standard reference substances of sucrose, stachyose, raffinose, and mulberry sugar were weighed precisely and prepared with 70% acetonitrile aqueous solution into the standard reference solution with a concentration of 0.5 mg/mL, respectively. The RGO powder was also weighed precisely, and prepared with 70% acetonitrile aqueous solution into the sample solution with a concentration of 1 mg/mL. The chromatographic column was Agilent ZORBOX NH2 (4.6 mm × 250 mm, 5 μm); the mobile phase was acetonitrile: water (7:3); the injection volume was 10 μL, the flow rate was 1.0 mL/min, and the temperature of column incubator was 40°C. The temperature of the detection was 50°C with RID. The types of oligosaccharides in RGO were determined by comparing the HPLC peaks of reference substance with those in RGO, and the content of oligosaccharides was calculated by external standard method.
The type and content of oligosaccharides in RGO were detected using high-performance liquid chromatography (HPLC) (Agilent1260), configured using a Refractive Index Detector (RID) (13 (link)). The standard reference substances of sucrose, stachyose, raffinose, and mulberry sugar were weighed precisely and prepared with 70% acetonitrile aqueous solution into the standard reference solution with a concentration of 0.5 mg/mL, respectively. The RGO powder was also weighed precisely, and prepared with 70% acetonitrile aqueous solution into the sample solution with a concentration of 1 mg/mL. The chromatographic column was Agilent ZORBOX NH2 (4.6 mm × 250 mm, 5 μm); the mobile phase was acetonitrile: water (7:3); the injection volume was 10 μL, the flow rate was 1.0 mL/min, and the temperature of column incubator was 40°C. The temperature of the detection was 50°C with RID. The types of oligosaccharides in RGO were determined by comparing the HPLC peaks of reference substance with those in RGO, and the content of oligosaccharides was calculated by external standard method.
acetonitrile
Adsorption
Anion Exchange Resins
Carbohydrates
Cation Exchange Resins
Charcoal, Activated
Chromatography
Clay
High-Performance Liquid Chromatographies
Morus
Oligosaccharides
Powder
Raffinose
Rehmannia glutinosa
Resins, Plant
Rhizome
stachyose
Sucrose
Expression of uniform [13C, 15N] labeled wild-type Asyn was carried out in E. coli BL21 (DE3)/pET28a-AS in modified Studier medium M (Studier 2005 (link)). The labeling medium contained 3.3 g/L [13C]glucose, 3 g/L [15N]ammonium chloride, 11 mL/L [13C, 15N]Bioexpress (Cambridge Isotope Laboratories, Inc., Tewksbury, MA), 1 mL/L BME vitamins (Sigma), and 90 μg/mL kanamycin. After a preliminary growth in medium containing natural abundance (NA) isotopes, the cells were transferred to the labeling medium at 37 °C to an OD600 of 1.2, at which point the temperature was reduced to 25 °C and protein expression induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) and grown for 15 h to a final OD600 of 4.1 and harvested.
Protein purification was done as described previously (Barclay et al. 2018 (link)). Briefly, cells were lysed chemically in the presence of Turbonuclease (Sigma) to digest nucleic acids. Purification began with a heat denaturation of the cleared lysate, followed by ammonium sulfate precipitation (Kloepper et al. 2006 (link)). The resolubilized protein was bound to QFF anion exchange resin (GE Healthcare Life Sciences, Marlborough, MA) and eluted using a linear gradient of 0.2–0.6 M NaCl. Fractions containing Asyn monomer, which eluted at about 0.3 M NaCl, were pooled, concentrated, and run over a 26/60 Sephacryl S-200 HR gel filtration column (GE Healthcare Life Sciences) equilibrated in 50 mM Tris-HCl, 100 mM NaCl, pH 8 buffer. Fractions were pooled, concentrated to ~20 mg/mL Asyn, and dialyzed at 4 °C into 10 mM Tris-HCl pH 7.6, 50 mM NaCl, 1 mM DTT, and stored at a concentration of ~14 mg/mL at −80 °C until use. Yields were 95 mg purified AS protein/L growth medium for the uniform [13C, 15N] labeled monomer.
Protein purification was done as described previously (Barclay et al. 2018 (link)). Briefly, cells were lysed chemically in the presence of Turbonuclease (Sigma) to digest nucleic acids. Purification began with a heat denaturation of the cleared lysate, followed by ammonium sulfate precipitation (Kloepper et al. 2006 (link)). The resolubilized protein was bound to QFF anion exchange resin (GE Healthcare Life Sciences, Marlborough, MA) and eluted using a linear gradient of 0.2–0.6 M NaCl. Fractions containing Asyn monomer, which eluted at about 0.3 M NaCl, were pooled, concentrated, and run over a 26/60 Sephacryl S-200 HR gel filtration column (GE Healthcare Life Sciences) equilibrated in 50 mM Tris-HCl, 100 mM NaCl, pH 8 buffer. Fractions were pooled, concentrated to ~20 mg/mL Asyn, and dialyzed at 4 °C into 10 mM Tris-HCl pH 7.6, 50 mM NaCl, 1 mM DTT, and stored at a concentration of ~14 mg/mL at −80 °C until use. Yields were 95 mg purified AS protein/L growth medium for the uniform [13C, 15N] labeled monomer.
Anion Exchange Resins
Buffers
Cells
Chloride, Ammonium
Culture Media
Escherichia coli
Gel Chromatography
Glucose
Isotopes
Kanamycin
Nucleic Acids
Proteins
Sodium Chloride
Sulfate, Ammonium
Tromethamine
Vitamins
Purolite® A502PS, a commercially available anion-exchange resin (IEX), was used in combination with an MF membrane. The IEX is a polystyrenic Macroporous, Type I Strong Base Anion Resin which is a Type 1, quaternary/ammonium with chloride ion. Its total exchange capacity (min) is 0.85 eq/L (18.6 kg/ft3), and its moisture retention is 66–72%. Particle sizes ranging between 425 and 600 µm were selected for this experiment. The authors found in their previous study that this range of particle sizes was effective in reducing the transmembrane pressure (TMP) of the submerged membrane hybrid system [19 (link)].
Ammonium
Anion Exchange Resins
Anions
Chloride, Ammonium
Chlorides
Hybrids
Pressure
Resins, Plant
Retention (Psychology)
Tissue, Membrane
The morphology of S. zooepidemicus and the surface area of anion-exchange resins were investigated under the scanning electron microscope (SEM) (JSM-IT 100, Jeol, Akishima, Japan). The method was adopted from Othman et al. (2018) [20 (link)] with slight modifications. The samples were prepared by fixation that was done with 4% (v/v) glutaraldehyde buffer for 12 h at 4 °C. The samples were then washed with 0.1 M sodium cacodylate buffer for 10 min. Next, the samples were fixed with 1% (w/v) osmium tetroxide for 2 h at 4 °C and washed once again. Then, the samples were dehydrated with increasing serial concentrations of acetone. The samples were placed in the sputter coater chamber after being mounted on an aluminum stub with a double stick of carbon tape. The samples were subsequently coated with a thin layer of metal gold/palladium (40–60 nm) and magnified at 2000× and 5000×.
Acetone
Aluminum
Anion Exchange Resins
Buffers
Cacodylate
Carbon
Glutaral
Gold
Metals
Osmium Tetroxide
Palladium
Scanning Electron Microscopy
Sodium
Top products related to «Anion Exchange Resins»
Sourced in United States
AG 1-X8 anion exchange resin is a strong base anion exchange resin used for the separation and purification of various ionic species. It is composed of a styrene-divinylbenzene copolymer matrix with quaternary ammonium functional groups. The resin has a high capacity for anions and is suitable for a wide range of applications in analytical and preparative chromatography.
Sourced in United States
DEAE-Sephadex A-25 is an anion-exchange resin. It is used for the purification and separation of biomolecules, such as proteins, enzymes, and nucleic acids, based on their charge properties.
Sourced in United States, United Kingdom
Anion exchange resin is a type of chromatographic material used for the separation and purification of negatively charged molecules, such as proteins, nucleic acids, and other biomolecules. The resin is composed of small, porous beads that contain positively charged functional groups, which can interact with and bind to the negatively charged target molecules. This allows for the selective separation and enrichment of the desired biomolecules from complex mixtures.
Sourced in United States
Dowex 1X2 is a strong-base anion exchange resin. It is a polymeric material with a polystyrene-divinylbenzene matrix and quaternary ammonium functional groups. Dowex 1X2 is designed for use in various chemical and industrial applications that require ion exchange capabilities.
Sourced in United States, Germany, United Kingdom, France, Italy, Israel, Czechia, Austria, Macao, India, Sao Tome and Principe, Switzerland, Denmark, Japan, Sweden, Poland, Australia, China, Spain
NaHCO3 is a chemical compound that is commonly used as a laboratory reagent. It is a white, crystalline powder with the chemical formula NaHCO3. NaHCO3 is a salt that is composed of sodium (Na+) and bicarbonate (HCO3-) ions.
Sourced in United States
The TopCount instrument is a high-performance microplate reader designed for sensitive and accurate detection of various assays. It utilizes advanced detection technologies to provide reliable data. The core function of the TopCount is to accurately measure and quantify luminescent, fluorescent, and radiometric signals in multiwell plate formats.
Sourced in United States
AG1-X8 is an anion exchange resin product manufactured by Bio-Rad. It is a strongly basic, gel-type anion exchange resin with a polystyrene-divinylbenzene matrix and quaternary ammonium functional groups.
Sourced in United States, United Kingdom, Germany
Potassium carbonate is a chemical compound with the formula K2CO3. It is a white, crystalline solid that is soluble in water. Potassium carbonate is commonly used in various industrial and laboratory applications.
HiTrap Q Sepharose FF is an anion exchange resin used for the purification of proteins and other biomolecules. It consists of a cross-linked agarose matrix with quaternary ammonium groups, which enable the binding and separation of negatively charged molecules. This resin is designed for medium-pressure chromatography applications.
Sourced in United States, Germany
Hexokinase is an enzyme that catalyzes the phosphorylation of glucose to glucose-6-phosphate, a key step in glycolysis. It plays a crucial role in the metabolism of carbohydrates.
More about "Anion Exchange Resins"
Anion exchange resins, also known as ion-exchange resins or anionic resins, are a class of materials widely used in various scientific applications, including water treatment, ion chromatography, and biochemical purification.
These resins are designed to selectively bind and exchange anions, making them invaluable tools for researchers studying ionic processes and developing new separation techniques.
One commonly used anion exchange resin is the AG 1-X8, a strongly basic resin that can be used for the separation and purification of a wide range of anionic species.
Another example is the DEAE-Sephadex A-25 anion-exchange resin, which is often employed in the purification of proteins and nucleic acids.
Anion exchange resins can be further categorized based on their functional groups, such as quaternary ammonium or tertiary amine groups, which determine their ion-exchange properties and selectivity.
The Dowex 1X2 resin is a popular strong base anion exchange resin, while the NaHCO3 (sodium bicarbonate) can be used as an eluent in ion chromatography applications.
The TopCount instrument, a highly sensitive scintillation counter, is often used in conjunction with anion exchange resins to analyze radioactive samples.
Additionally, the AG1-X8 resin can be used in the determination of hexokinase activity, a key enzyme in glucose metabolism, through the separation and detection of its reaction products.
Potassium carbonate (K2CO3) is another compound that can be used in the preparation and regeneration of anion exchange resins, as it helps to maintain the desired pH and ionic conditions.
The HiTrap Q Sepharose FF anion exchange resin is a versatile tool for the purification of proteins, nucleic acids, and other biomolecules, leveraging the selective binding and exchange properties of anion exchange resins.
By understanding the diverse applications and characteristics of anion exchange resins, researchers can optimize their experimental protocols, improve the efficiency of their purification and separation processes, and drive scientific discovery to new heights.
These resins are designed to selectively bind and exchange anions, making them invaluable tools for researchers studying ionic processes and developing new separation techniques.
One commonly used anion exchange resin is the AG 1-X8, a strongly basic resin that can be used for the separation and purification of a wide range of anionic species.
Another example is the DEAE-Sephadex A-25 anion-exchange resin, which is often employed in the purification of proteins and nucleic acids.
Anion exchange resins can be further categorized based on their functional groups, such as quaternary ammonium or tertiary amine groups, which determine their ion-exchange properties and selectivity.
The Dowex 1X2 resin is a popular strong base anion exchange resin, while the NaHCO3 (sodium bicarbonate) can be used as an eluent in ion chromatography applications.
The TopCount instrument, a highly sensitive scintillation counter, is often used in conjunction with anion exchange resins to analyze radioactive samples.
Additionally, the AG1-X8 resin can be used in the determination of hexokinase activity, a key enzyme in glucose metabolism, through the separation and detection of its reaction products.
Potassium carbonate (K2CO3) is another compound that can be used in the preparation and regeneration of anion exchange resins, as it helps to maintain the desired pH and ionic conditions.
The HiTrap Q Sepharose FF anion exchange resin is a versatile tool for the purification of proteins, nucleic acids, and other biomolecules, leveraging the selective binding and exchange properties of anion exchange resins.
By understanding the diverse applications and characteristics of anion exchange resins, researchers can optimize their experimental protocols, improve the efficiency of their purification and separation processes, and drive scientific discovery to new heights.