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Bioplex
Bioplex
Bioplex refers to the complex biological systems involved in various life processes, such as cell signaling, protein interactions, and metabolic pathways.
These intricate networks of biomolecular interactions are crucial for understanding the mechanisms underlying health and disease.
The study of bioplex systems often involves the use of experimental techniques like proteomics, metabolomics, and systems biology to elucidate the dynamic and interconnected nature of biological components.
Researchers leveraging AI-powered tools like PubCompare.ai can streamline their bioplex research, enhacing reproducibility and identifing the most effective products and methods from the literature, preprints, and patents.
These intricate networks of biomolecular interactions are crucial for understanding the mechanisms underlying health and disease.
The study of bioplex systems often involves the use of experimental techniques like proteomics, metabolomics, and systems biology to elucidate the dynamic and interconnected nature of biological components.
Researchers leveraging AI-powered tools like PubCompare.ai can streamline their bioplex research, enhacing reproducibility and identifing the most effective products and methods from the literature, preprints, and patents.
Most cited protocols related to «Bioplex»
NR4A2 protein, human
Proteins
Staphylococcal Protein A
Trypsin
The steps in assay validation were similar to recently developed bead-based multiplex immunoassays for CMV, EBV, and RSV, with minor modifications as described below [16 (link), 17 (link)]. For the multiplex bead-based immune assay the following antigens obtained from Sino Biological were used: SARS-CoV-2 monomeric spike S1 (40591-V08H), RBD (40592-V08B), and nucleoprotein (N) (40588-V08B). Microplex fluorescent beads were activated in 50 mM 2-(N-morpholino)ethanesulfonic acid (MES) pH 5.5. The proteins were diluted to a concentration of 0.2 mg/mL in phosphate-buffered saline (PBS) pH 7.4 and added at 5 µg per 75 µL of activated beads.
An internal reference sample was created by pooling 13 sera of COVID-19 patients with varying immunoglobulin G (IgG) concentrations. An arbitrary antibody concentration unit of 100 was assigned on the basis of the mean fluorescence intensity (MFI) signal in the upper limit of linearity of a 3-fold serial dilution of the reference sample.
Sera (25 µL) diluted 1:400 and 1:8000 in SM01 buffer (Surmodics) plus 2% fetal calf serum were incubated with antigen-coated beads for 45 minutes at room temperature at 750 rpm in the dark. Following incubation, samples were washed 3 times with PBS, incubated with phycoerythrin-conjugated goat anti-human IgG for 30 minutes and washed. Samples were acquired on an LX200 or FM3D (Luminex). MFI was converted to arbitrary units (AU/mL) by interpolation from a 5-parameter logistic standard curve, using Bioplex Manager 6.2 (Bio-Rad Laboratories) software and exported to Microsoft Excel.
An internal reference sample was created by pooling 13 sera of COVID-19 patients with varying immunoglobulin G (IgG) concentrations. An arbitrary antibody concentration unit of 100 was assigned on the basis of the mean fluorescence intensity (MFI) signal in the upper limit of linearity of a 3-fold serial dilution of the reference sample.
Sera (25 µL) diluted 1:400 and 1:8000 in SM01 buffer (Surmodics) plus 2% fetal calf serum were incubated with antigen-coated beads for 45 minutes at room temperature at 750 rpm in the dark. Following incubation, samples were washed 3 times with PBS, incubated with phycoerythrin-conjugated goat anti-human IgG for 30 minutes and washed. Samples were acquired on an LX200 or FM3D (Luminex). MFI was converted to arbitrary units (AU/mL) by interpolation from a 5-parameter logistic standard curve, using Bioplex Manager 6.2 (Bio-Rad Laboratories) software and exported to Microsoft Excel.
Antibodies, Anti-Idiotypic
Antigens
Biological Assay
Biopharmaceuticals
bioplex
COVID 19
ethane sulfonate
Fetal Bovine Serum
Fluorescence
Goat
Homo sapiens
Immunoassay
Immunoglobulin G
Immunoglobulins
isononanoyl oxybenzene sulfonate
Morpholinos
Nucleoproteins
Patients
Phosphates
Phycoerythrin
Proteins
Saline Solution
SARS-CoV-2
Serum
Technique, Dilution
AN 12
Biopharmaceuticals
bioplex
Mass Spectrometry
Proteins
The BioPlex 2.0 network and its underlying data are available to the scientific community in several formats. First, all interactions in the BioPlex network have been deposited in the BioGRID protein interaction database. Second, we have created a website devoted to the project (http://bioplex.hms.harvard.edu ) which provides tools to download i) the interactions that make up BioPlex 1.0 and 2.0; ii) a customized viewer that enables browsing of either network to examine the interactions of specific proteins; iii) an interface for download of nearly 12,000 individual RAW files containing mass spectrometry data from individual AP-MS experiments; and iv) an R package and web-based tool for performing CompPASS analyses. Third, the BioPlex 2.0 network as bait-prey pairs has been incorporated into NDEx40 (link), a web-based platform for biological Network Data Exchange. Fourth, our RAW files have been submitted for inclusion in ProteomicsDB45 (link). Finally, all RAW files (3Tb) from this study will be provided to investigators upon request using investigator-provided hard drives. Finally, a table in. tsv format containing all proteins and spectral count information for all 5891 AP-MS experiments reported here is available for download at the BioPlex website.
AN 12
Biopharmaceuticals
bioplex
Mass Spectrometry
Proteins
Most recents protocols related to «Bioplex»
Mice were infected as described above and were euthanised 24-h post infection. The bladders were collected and homogenised in the presence of a protease inhibitor cocktail (Roche). The samples were spun at 12,000 x g for 20 min at 4 °C, and supernatants were stored at −80 °C. Cytokine measurements were performed on 4–9 samples per group using the Bio-Plex Pro Mouse Cytokine 23–Plex Group 1 kit as per manufacturer’s instructions (Bio-Rad).
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The BioPlex 2200 SARS-CoV-2 IgG Panel (BIO-RAD, Hercules, California, USA) is a multiplex immunoassay for the qualitative detection and semi-quantitative differentiation of IgG against the RBD, S1, S2 and N proteins of SARS-CoV-2. Results < 10U/mL are reported as negative and ≥ 10 U/mL as positive. The measuring range for the assay is 1–100 U/mL. Results outside of this range are reported as < 1 U/mL or > 100 U/mL.
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Plasma samples were taken from cancer cachexia mice at 24 h after the evaluation of behavioral phenotypes. Cytokines, chemokines, and growth factors in plasma samples were quantified by the Bio-Plex® Multiplex System (Bio-Rad Laboratories Inc., CA, USA). In this study, the Bio-Plex Pro™ Mouse Cytokine 23-plex Assay (#64,140,214, Bio-Rad Laboratories Inc.) and Bio-Plex Pro™ Mouse Cytokine 9-plex Assay (#64,174,947, Bio-Rad Laboratories Inc.) were used.
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The levels of bone marker-related proteins sclerostin (SOST), osteocalcin (OCN) and osteoprotegerin (OPG) in both CLI patient serum and healthy controls, and conditioned culture media were determined via a multiplex array analysis using a Milliplex Luminex xMAP™ bone marker panel (Merck Millipore, Burlington, MA, USA), performed as per manufacturer’s protocol.
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The concentrations of IL-1 , IL-6, IL-10, IL-17A, IL-17F, IL-22, IL-23, IL-33, IFN- , and TNF- were measured using commercial Multiplex kits (ThermoFisher) according to the instructions of the manufacturer.
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Top products related to «Bioplex»
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The Bio-Plex 200 is a multiplex detection system that utilizes magnetic beads and dual-laser fluorescence detection to quantify multiple analytes in a single sample. The system is designed to enable simultaneous measurement of up to 100 different biomolecules in a small sample volume.
Sourced in United States, United Kingdom, Germany, Canada, France, Belgium, Switzerland, China, Austria, Sweden, Australia, Japan, Italy
The Bio-Plex 200 system is a multiplex immunoassay platform that allows for the simultaneous detection and quantification of multiple analytes in a single sample. The system utilizes fluorescently-labeled magnetic beads and a dual-laser detection system to perform high-throughput, multiplexed analyses.
Sourced in United States, Italy, Germany, Belgium, Japan, Canada
Bio-Plex is a multiplex assay platform that allows for the simultaneous detection and quantification of multiple analytes in a single sample. It utilizes magnetic beads coated with specific antibodies to capture and measure target molecules. The Bio-Plex system provides a flexible and efficient solution for high-throughput analysis of various biomolecules, enabling researchers to obtain comprehensive data from minimal sample volumes.
Sourced in United States, Germany, United Kingdom, Canada, Sweden, Italy
Bio-Plex Manager software is a data analysis tool designed for use with Bio-Rad's Bio-Plex multiplex assay system. It provides functionality for data acquisition, analysis, and visualization.
Sourced in United States, France, Canada, Japan, United Kingdom, Italy
Bio-Plex Manager 6.1 software is a data analysis software for the Bio-Plex multiplex assay system. It is designed to acquire, analyze, and manage data from Bio-Plex assays.
Sourced in United States, Australia, United Kingdom, France, Czechia
The Bio-Plex 200 instrument is a multiplex immunoassay platform that allows for the simultaneous detection and quantification of multiple analytes in a single sample. It utilizes color-coded magnetic beads coated with specific capture antibodies to enable the measurement of up to 100 different analytes in a single well.
Sourced in United States, Japan, France, Germany, Netherlands
The Bio-Plex system is a multiplex assay platform that enables the simultaneous detection and quantification of multiple target analytes in a single sample. The system utilizes xMAP technology, which combines fluorescent-coded beads, flow cytometry, and laser-based detection to provide a powerful and flexible solution for a wide range of bioassay applications.
Sourced in United States, Germany, Canada, France, Morocco, Australia, United Kingdom
The Milliplex MAP Kit is a multiplex assay platform developed by Merck Group. The kit enables the simultaneous detection and measurement of multiple analytes in a single sample. The core function of the Milliplex MAP Kit is to provide a versatile and efficient tool for researchers to conduct multiplexed biomarker analysis.
Sourced in United States, Canada
The BioPlex 2200 is an automated multiplex assay system designed for the quantitative analysis of multiple analytes in a single sample. It utilizes magnetic bead-based technology to perform simultaneous measurements of various biomarkers, proteins, and other molecules. The system offers high-throughput capabilities and is suitable for a wide range of applications in clinical diagnostics, research, and drug development.
Sourced in United States, Germany, Canada
The Bio-Plex MAGPIX Multiplex Reader is a compact, automated instrument that utilizes magnetic bead-based technology to perform multiplexed analysis of various analytes in a single sample. The instrument is designed to detect and quantify multiple targets simultaneously, providing efficient and high-throughput data generation.
More about "Bioplex"
Bioplex refers to the complex biological systems involved in various life processes, such as cell signaling, protein interactions, and metabolic pathways.
These intricate networks of biomolecular interactions are crucial for understanding the mechanisms underlying health and disease.
The study of bioplex systems often involves the use of experimental techniques like proteomics, metabolomics, and systems biology to elucidate the dynamic and interconnected nature of biological components.
Researchers can leverage AI-powered tools like PubCompare.ai to streamline their bioplex research, enhancing reproducibility and identifying the most effective products and methods from the literature, preprints, and patents.
This cutting-edge tool enables intelligent comparisons of research protocols, helping scientists locate the best approaches for their bioplex experiments.
The Bio-Plex 200 system, Bio-Plex, and Bio-Plex Manager software are commonly used in bioplex research.
The Bio-Plex 200 instrument is a powerful multiplex reader that can simultaneously detect and quantify multiple analytes in a single sample.
The Bio-Plex Manager 6.1 software provides advanced data analysis and visualization capabilities to support bioplex research.
Milliplex Map Kits are another useful tool for bioplex studies, allowing for the simultaneous measurement of multiple analytes in a single well.
The BioPlex 2200 system and Bio-Plex MAGPIX Multiplex Reader are also valuable resources for researchers working with bioplex systems.
By leveraging these advanced technologies and AI-powered tools, scientists can streamline their bioplex research, enhance reproducibility, and gain deeper insights into the complex biological systems that underlie health and disease.
These intricate networks of biomolecular interactions are crucial for understanding the mechanisms underlying health and disease.
The study of bioplex systems often involves the use of experimental techniques like proteomics, metabolomics, and systems biology to elucidate the dynamic and interconnected nature of biological components.
Researchers can leverage AI-powered tools like PubCompare.ai to streamline their bioplex research, enhancing reproducibility and identifying the most effective products and methods from the literature, preprints, and patents.
This cutting-edge tool enables intelligent comparisons of research protocols, helping scientists locate the best approaches for their bioplex experiments.
The Bio-Plex 200 system, Bio-Plex, and Bio-Plex Manager software are commonly used in bioplex research.
The Bio-Plex 200 instrument is a powerful multiplex reader that can simultaneously detect and quantify multiple analytes in a single sample.
The Bio-Plex Manager 6.1 software provides advanced data analysis and visualization capabilities to support bioplex research.
Milliplex Map Kits are another useful tool for bioplex studies, allowing for the simultaneous measurement of multiple analytes in a single well.
The BioPlex 2200 system and Bio-Plex MAGPIX Multiplex Reader are also valuable resources for researchers working with bioplex systems.
By leveraging these advanced technologies and AI-powered tools, scientists can streamline their bioplex research, enhance reproducibility, and gain deeper insights into the complex biological systems that underlie health and disease.