The largest database of trusted experimental protocols

Capsule

Capsules are small, enclosed containers used to deliver medications or other substances.
They typically consist of a gelatin or other polymer shell that holds the active ingredient.
Capsules provide a convenient and precise way to administer drugs, vitamins, or dietary supplements.
They can help protect the contents from environmental factors, mask unpleasant flavors, and facilitate swallowing.
Capsules come in a variety of sizes and shapes to suit different dosage requirements.
Researchers and healthcare providers utilize capsules to optimize drug delivery and improve patient compliance.

Most cited protocols related to «Capsule»

To cover a large portion of the known bacterial diversity within this species (Table S2), a total of 462 E. coli strains from multiple healthy and diseased sources were investigated. We scored as pathogenic those bacteria isolated from diseased hosts or with known virulence determinants (see bottom of Table S2) and all others as non-pathogens. One focus of the collection consisted of pathogens from both humans and domesticated animals that had been classified as EHEC (41 isolates), EPEC (20), EAEC (9), or ETEC (20) on the basis of virulence determinants (Nataro and Kaper, 1998 (link)) or APEC (13) on the basis of typical disease in domesticated animals. To add geographical as well as host diversity, and to expand the numbers of non-pathogens, the collection included all 72 isolates from the ECOR collection (Ochman and Selander, 1984 (link)), 15 isolates that represent the known diversity of E. coli from healthy wild mammals in Australia (Gordon et al., 2002 (link)) and 114 isolates from patients with diarrhoea in Ghana plus their close contacts including food handlers. We also included 61 Shigella from all known serotypes and species, 38 EIEC of different serotypes and 46 isolates from a variety of clonal groupings that express the K1 capsular polysaccharide (Achtman and Pluschke, 1986 (link)). Additional details including geographic origin are in Table S2.
Sequence-based phylogenetic analysis showed that two E. coli isolates (isolates RL325/96 and Z205 from a dog and a parrot respectively) differed markedly from the remaining isolates (Fig. 2). These strains clearly belong to E. coli according to biochemical, serological and metabolic typing schemes and by 16S rDNA sequences. Based on the MLST data, they represent the deepest known evolutionary lineages in this species. Because of their extensive sequence divergence from the vast majority of E. coli strains, they were excluded from subsequent analysis.
Publication 2006
Animal Diseases Animals, Domestic Bacteria Biological Evolution Capsule Clone Cells Diarrhea DNA, Ribosomal Enterohemorrhagic Escherichia coli Enteropathogenic Escherichia coli Enterotoxigenic Escherichia coli Escherichia coli Food Homo sapiens Mammals Parrots Pathogenicity Patients Polysaccharides Shigella Strains Virulence Factors
The Iso-Seq method for sequencing full-length transcripts was developed by PacBio during the same time period as the genome assembly. We therefore used this technique to improve characterization of transcript isoforms expressed in cattle tissues using a diverse set of tissues collected from L1 Dominette 0 1449 upon euthanasia. The data were collected using an early version of the Iso-Seq library protocol [26 ] as suggested by PacBio. Briefly, RNA was extracted from each tissue using Trizol reagent as directed (Thermo Fisher). Then 2 μg of RNA were selected for PolyA tails and converted into complementary DNA (cDNA) using the SMARTer PCR cDNA Synthesis Kit (Clontech). The cDNA was amplified in bulk with 12–14 rounds of PCR in 8 separate reactions, then pooled and size-selected into 1–2, 2–3, and 3–6 kb fractions using the BluePippin instrument (Sage Science). Each size fraction was separately re-amplified in 8 additional reactions of 11 PCR cycles. The products for each size fraction amplification were pooled and purified using AMPure PB beads (Pacific Biosciences) as directed, and converted to SMRTbell libraries using the Template Prep Kit v1.0 (PacBio) as directed. Iso-Seq was conducted for 22 tissues including abomasum, aorta, atrium, cerebral cortex, duodenum, hypothalamus, jejunum, liver, longissimus dorsi muscle, lung, lymph node, mammary gland, medulla oblongata, omasum, reticulum, rumen, subcutaneous fat, temporal cortex, thalamus, uterine myometrium, and ventricle from the reference cow, as well as the testis of her sire. The size fractions were sequenced in either 4 (for the smaller 2 fractions) or 5 (for the largest fraction) SMRTcells on the RS II instrument. Isoforms were identified using the Cupcake ToFU pipeline [27 ] without using a reference genome.
Short-read–based RNA-seq data derived from tissues of Dominette were available in the GenBank database because her tissues have been a freely distributed resource for the research community. To complement and extend these data and to ensure that the tissues used for Iso-Seq were also represented by RNA-seq data for quantitative analysis and confirmation of isoforms observed in Iso-Seq, we generated additional data, avoiding overlap with existing public data. Specifically, the TruSeq stranded mRNA LT kit (Illumina, Inc.) was used as directed to create RNA-seq libraries, which were sequenced to ≥30 million reads for each tissue sample. The Dominette tissues that were sequenced in this study include abomasum, anterior pituitary, aorta, atrium, bone marrow, cerebellum, duodenum, frontal cortex, hypothalamus, KPH fat (internal organ fat taken from the covering on the kidney capsule), lung, lymph node, mammary gland (lactating), medulla oblongata, nasal mucosa, omasum, reticulum, rumen, subcutaneous fat, temporal cortex, thalamus, uterine myometrium, and ventricle. RNA-seq libraries were also sequenced from the testis of her sire. All public datasets, and the newly sequenced RNA-seq and Iso-Seq datasets, were used to annotate the assembly, to improve the representation of low-abundance and tissue-specific transcripts, and to properly annotate potential tissue-specific isoforms of each gene.
Full text: Click here
Publication 2020
Abomasum Anabolism Aorta Bone Marrow Capsule Cattle cDNA Library Cerebellum Cerebral Ventricles Cortex, Cerebral Dietary Fiber DNA, Complementary Duodenum Euthanasia Genes Genome Heart Atrium Hypothalamus Jejunum Kidney Liver Lobe, Frontal Lung Mammary Gland Medulla Oblongata Muscle Tissue Myometrium Nasal Mucosa Nodes, Lymph Omasum Pituitary Hormones, Anterior Poly(A) Tail Protein Isoforms Reticulum RNA, Messenger RNA-Seq Rumen Subcutaneous Fat Temporal Lobe Testis Thalamus Tissues Tissue Specificity Tofu trizol Uterus

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2021
Actins Animals Antibodies Biopharmaceuticals Capsule Catabolism Cataract Cells DNA Replication Epithelium Epitopes Eye Fibrosis Fluorescent Antibody Technique Fluorescent Dyes Freezing Gene Expression Genes Homo sapiens Immunoglobulins Institutional Animal Care and Use Committees Lens, Crystalline Mice, Inbred C57BL Microscopy, Confocal Mus Operative Surgical Procedures Protein Denaturation Proteins RNA-Seq Smooth Muscles Tissues Training Programs Vision Western Blotting
Litters chosen for testing contained more than seven pups for BTBR (7.8±1.05), B6 (7.1±0.58) and FVB/NJ (7.5±0.34), and more than five pups for 129X1 (5.5±0.40; a strain known for small litters). One female and one male from each litter of BTBR, B6, FVB/NJ and 129X1 mice (n = 10 litters each strain) were used for baseline measurements of the ultrasonic vocalizations from pnd 2 to 12. Body weights and body temperatures of pups were measured after the ultrasonic vocalization test on pnd 2, 4, 6, 8 and 12. On each day of testing, each pup was placed into an empty plastic container (diameter, 5 cm; height 10 cm), located inside a sound-attenuating styrofoam box, and assessed for USVs during a five minute test. At the end of the five minute recording session, each pup was weighed and its axillary temperature measured by gentle insertion of the thermal probe in the skin pocket between upper foreleg and chest of the animal for about 30 seconds (Microprobe digital thermometer with mouse probe, Stoelting Co., Illinois, USA). No differences in patterns of calling were detected in a comparison of male and female pups, therefore data were collapsed across sex.
An Ultrasound Microphone (Avisoft UltraSoundGate condenser microphone capsule CM16, Avisoft Bioacoustics, Berlin, Germany) sensitive to frequencies of 10–180 kHz, recorded the pup vocalizations in the sound-attenuating chamber. The microphone was placed through a hole in the middle of the cover of the styrofoam sound-attenuating box, about 20 cm above the pup in its plastic container. The temperature of the room was maintained at 22±1°C. Vocalizations were recorded using Avisoft Recorder software (Version 3.2). Settings included sampling rate at 250 kHz; format 16 bit. For acoustical analysis, recordings were transferred to Avisoft SASLab Pro (Version 4.40) and a fast Fourier transformation (FFT) was conducted. Spectrograms were generated with an FFT-length of 1024 points and a time window overlap of 75% (100% Frame, Hamming window). The spectrogram was produced at a frequency resolution of 488 Hz and a time resolution of 1 ms. A lower cut-off frequency of 15 kHz was used to reduce background noise outside the relevant frequency band to 0 dB. Call detection was provided by an automatic threshold-based algorithm and a hold-time mechanism (hold time: 0.01 s). An experienced user checked the accuracy of call detection, and obtained a 100% concordance between automated and observational detection. Parameters analyzed for each test day included number of calls, duration of calls, qualitative and quantitative analyses of sound frequencies measured in terms of frequency and amplitude at the maximum of the spectrum.
Waveform patterns of calls were examined in depth in twenty sonograms collected from every strain, one from each of the pups tested. The sonograms were one minute in length and selected from recordings at postnatal day 8. We classified 3633 BTBR calls, 2333 B6 calls, 1806 129X1 calls and 2575 FVB/NJ calls. Each call was identified as one of 10 distinct categories, based on internal pitch changes, lengths and shapes, using previously published categorizations [21] (link), [22] (link), [24] (link). Classification of USVs included ten waveform patterns described below, and illustrated visually in Figure 2 and S1 and as audiofiles (Sounds S1, S2, S3, S4, S5, S6, S7, S8, S9, S10) in Supporting Information.
Inter-rater reliability in scoring the call categories was 98%. Call category data were subjected to two different analyses: a) strain-dependent effects on the frequency and duration of the vocalizations emitted by each subject at pnd 8 b) strain-dependent effects on the probability of producing calls from each of the ten categories of USV, as described below under Statistical analysis.
Full text: Click here
Publication 2008
Acoustics Animals Axilla Body Weight Capsule Chest Females Fingers Males Mice, House Neoplasm Metastasis Patient Holding Stretchers Reading Frames Skin Sound Strains styrofoam Thermometers Ultrasonics Ultrasonography
The bacterial virulent protein sequences were retrieved from the SWISS-PROT [20 (link)] and VFDB (an integrated and comprehensive database of virulence factors of bacterial pathogens, [21 (link)]). SWISS-PROT sequences were retrieved using keywords such as virulence, adhesin, adhesion, adherence, toxin, invasion, capsule and other terms related to virulence factors. The VFDB and SWISS-PROT sequences were screened strictly in order to obtain a high quality dataset. First, the sequences were filtered to remove entries annotated as "Probable", Putative", "By similarity", "Fragments" "Hypothetical", "Unknown" and "Possible". The filtering yielded 1756 annotated virulent protein sequences (henceforth referred to as positive dataset).
For training with non-virulent protein sequences, we selected 3000 annotated protein sequences of bacterial enzymes and other non-virulent proteins from SWISS-PROT database (these sequences are henceforth referred to as negative dataset). The negative dataset sequences were mainly chosen from the bacterial proteomes, the virulent protein sequences of which are included in the positive dataset.
Full text: Click here
Publication 2008
Adhesins, Bacterial Amino Acid Sequence Bacteria Bacterial Proteins Capsule Enzymes Proteome Toxins, Biological Virulence Virulence Factors

Most recents protocols related to «Capsule»

Example 1

As a general procedure, shikonin or a composition comprising shikonin or a derivative thereof is formulated in capsules, optionally in combination with lecithin (phospholipids, comprising primarily phosphatidylcholine) (e.g., at a shikonin-to-lecithin weight ratio of about 1:1). The shikonin or derivative thereof may be substantially pure (from a synthetic or natural source) or a part of an extract of a plant, such as Lithospermum erythrorhizon, Arnebia euchroma or another member of the borage family.

Using the above general procedure, an extract of purple gromwell (Lithospermum erythrorhizon) root (zicao) was prepared using an appropriate solvent, followed by spray drying and sieving, to obtain a purple powder. 175 mg of the powdered purple gromwell extract, containing about 30% shikonin and/or derivatives thereof, was placed with an equal weight of lecithin (Lipoid® PS P 20×, obtained from Lipoid GmbH) in Capsugel® delayed release (DR) capsules.

As an alternative to capsules, a syrup was prepared comprising lecithin and shikonin (95% purity) at a 5:1 lecithin:shikonin ratio, 44% alcohol as solvent, and honey.

Based on literature reports, toxicity of shikonin is not expected at dosages of less than 8 grams per day.

Full text: Click here
Patent 2024
Arnebia Borago Capsule Coronavirus Infections derivatives Ethanol Family Member Honey Lecithin Lithospermum Phospholipids Plant Extracts Plant Roots shikonin Solvents
Not available on PMC !

Example 4

A male 58-year-old subject suffering from a migraine ingested a capsule comprising 1000 mg citric acid and a capsule comprising 1200 mg KNO3, 200 mg elemental magnesium, and 50 mg elemental zinc. Within 5 minutes of ingesting both capsules, the subject saw alleviation of migraine symptoms. 30 minutes after ingesting the capsules, the subject reported that the migraine symptoms had disappeared.

Full text: Click here
Patent 2024
Capsule Citric Acid Headache Magnesium Males Migraine Disorders Zinc

Example 13

Batch analytical data for Formula 21 was determined and recorded in Table 15. Results were recorded at time, T=0 and again at time, T=1 month at a temperature of 40° C. and 75% relative humidity (RH).

TABLE 15
Exemplary Liquisoft Composition
Matrix Formulation
Results at T = 1 months
Results at Initial T = 040° C./75% RH
Assay Results
Dextromethorphan Hbr98.0% label claim100.4% label claim
Menthol97.0% label claim100.4% label claim
Degradation Products Results
Dextromethorphan HbrRRT 1.09: 0.05%RRT 1.09: 0.05%
Total: 0.05%Total 0.05%
MentholNone DetectedRRT 1.15: 0.1%;
RRT 1.73: 0.2%,
Total 0.03%
Dissolution Study Results
Dextromethorphan HBrDextromethorphan HBr
15 minutes: 99%15 minutes: 99%
30 minutes: 98%30 minutes: 98%
45 minutes: 98%45 minutes: 98%
60 minutes: 98%60 minutes: 98%

Full text: Click here
Patent 2024
Biological Assay Capsule Dextromethorphan Hydrobromide Humidity Menthol
Not available on PMC !

Example 1

Exemplary capsule shell and matrix compositions useful for producing Liquisoft capsules as described herein are shown in Table 4. Composition components are set forth by weight percentage of the total weight of the composition. Such compositions may be encapsulated using rotary die encapsulation as described herein.

Formulas 1 and 2 were the first shell formulations developed to achieve faster disintegration time and prevent crosslinking of the gelatin shell with matrix fill components.

TABLE 4
Exemplary Liquisoft Composition
Capsule Shell Formulation
ComponentFormula 1Formula 2
Gelatin, 250 Bloom24.3
Gelatin, 150 Bloom29.2
Gelatin, 100 Bloom 4.9
Gelatin Hydrolysate
Hydrolyzed Collagen
Powdered Cellulose 1.9
Maltitol25.7
Glycerol32.019.1
Xylitol 4.8
Sucralose
Citric Acid 0.5
Flavors 0.5
Water32.325.0
TOTAL100%100%
VISCOSITY3497 cP

Full text: Click here
Patent 2024
Capsule Cellulose Citric Acid Collagen Type I Flavor Enhancers Gelatins Glycerin maltitol sucralose Viscosity Xylitol
Not available on PMC !

Example 3

Raw materialAmount mg/capsule
vicagrel15
pregaletinized starch49.5
lactose140
sodium carboxymethyl starch20
hydroxypropyl methylcellulose5
waterq.s
sodium stearyl fumarate0.5
total230

The pulverized vicagrel salt was placed with pregelatinized starch, lactose, and sodium carboxymethyl starch in a fluidized bed, fluidized mixing is started for 10 min, and 5% hydroxypropyl methylcellulose is prepared as a binder. At an air inlet temperature of 80° C., the binder was sprayed while maintaining a bed temperature at 40-50° C. The formed particles were dried for 30 min while the bed temperature was maintained at 50-60° C., and were discharged. Sodium stearyl fumarate was added and mixed for 5 min, and the particles were filled into capsules.

Full text: Click here
Patent 2024
Capsule carboxymethyl starch Fumarate Hypromellose Lactose sodium carboxymethyl starch Sodium Chloride sodium stearyl fumarate Starch vicagrel

Top products related to «Capsule»

Sourced in United States, Germany, China, Sao Tome and Principe, United Kingdom, India, Japan, Macao, Canada, France, Italy, Switzerland, Egypt, Poland, Hungary, Denmark, Indonesia, Singapore, Sweden, Belgium, Malaysia, Israel, Spain, Czechia
STZ is a laboratory equipment product manufactured by Merck Group. It is designed for use in scientific research and experiments. The core function of STZ is to serve as a tool for carrying out specific tasks or procedures in a laboratory setting. No further details or interpretation of its intended use are provided.
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
Sourced in Germany, United States, Italy, India, China, United Kingdom, France, Poland, Spain, Switzerland, Australia, Canada, Brazil, Sao Tome and Principe, Ireland, Belgium, Macao, Japan, Singapore, Mexico, Austria, Czechia, Bulgaria, Hungary, Egypt, Denmark, Chile, Malaysia, Israel, Croatia, Portugal, New Zealand, Romania, Norway, Sweden, Indonesia
Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.
Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan
Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
Sourced in United States, United Kingdom, Germany, China, Canada, Japan, Italy, France, Belgium, Australia, Uruguay, Switzerland, Israel, India, Spain, Denmark, Morocco, Austria, Brazil, Ireland, Netherlands, Montenegro, Poland
Matrigel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins. It is widely used as a substrate for the in vitro cultivation of cells, particularly those that require a more physiologically relevant microenvironment for growth and differentiation.
Sourced in Japan, United States, Germany, United Kingdom, China, France
The Hitachi H-7650 is a transmission electron microscope (TEM) designed for high-resolution imaging of materials. It provides a core function of nanoscale imaging and analysis of a wide range of samples.
Sourced in United States
The MPMS-XL SQUID magnetometer is a highly sensitive instrument designed to measure the magnetic properties of materials. It utilizes a Superconducting Quantum Interference Device (SQUID) to detect and quantify extremely small magnetic fields. The MPMS-XL is capable of performing measurements over a wide range of temperatures and magnetic fields, making it a versatile tool for materials research and characterization.
Sourced in United States, China, Germany, United Kingdom, Canada, Japan, France, Netherlands, Montenegro, Switzerland, Austria, Australia, Colombia, Spain, Morocco, India, Azerbaijan
Matrigel is a complex mixture of extracellular matrix proteins derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It is widely used as a basement membrane matrix to support the growth, differentiation, and morphogenesis of various cell types in cell culture applications.
Sourced in Germany, United States, Italy, United Kingdom, France, Spain, China, Poland, India, Switzerland, Sao Tome and Principe, Belgium, Australia, Canada, Ireland, Macao, Hungary, Czechia, Netherlands, Portugal, Brazil, Singapore, Austria, Mexico, Chile, Sweden, Bulgaria, Denmark, Malaysia, Norway, New Zealand, Japan, Romania, Finland, Indonesia
Formic acid is a colorless, pungent-smelling liquid chemical compound. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid is widely used in various industrial and laboratory applications.

More about "Capsule"

Capsules, also known as gelatin capsules or soft gel capsules, are small, enclosed containers used to deliver a variety of substances, including medications, vitamins, and dietary supplements.
These versatile vessels typically consist of a shell made from gelatin or other polymers that enclose the active ingredient.
Capsules offer a convenient and precise way to administer drugs, providing protection from environmental factors, masking unpleasant flavors, and facilitating swallowing.
Researchers and healthcare providers utilize capsules to optimize drug delivery and improve patient compliance.
Beyond medications, capsules can also be used to encapsulate other substances, such as cell culture media (e.g., DMEM), extracellular matrix components (e.g., Matrigel), and analytical reagents (e.g., STZ, FBS, acetonitrile, methanol, formic acid).
The unique properties of capsules make them useful in a wide range of applications, from pharmaceutical formulations to cell culture and analytical procedures.
The size and shape of capsules can be tailored to suit different dosage requirements, allowing for customized delivery.
This versatility makes capsules a valuable tool in the fields of pharmacology, biotechnology, and beyond.
Whether you're exploring new drug delivery methods, optimizing cell culture protocols, or conducting analytical experiments, understanding the capabilities of capsules can help enhance the reproducibility and accuracy of your research.
By incorporating the insights gained from the MeSH term description and metadescription, you can leverage the power of capsules to propel your work forward.