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Cetyl alcohol

Cetyl alcohol, a saturated fatty alcohol with the chemical formula CH3(CH2)14CH2OH, is a widely used ingredient in a variety of cosmetic and pharmaceutical products.
It serves as an emollient, emulsifier, and thickening agent, contributing to the texture and stability of formulations.
Cetyl alcohol is commonly found in moisturizers, creams, lotions, shampoos, and other personal care items.
Its unique properties make it a valuable component in the development of effective and well-tolerated topical preparations.
Reasearchers and formulators can leverage PubCompare.ai's AI-driven platform to quickly identify the best protocols and procedures for working with cetyl alcohol, streamlining their experriments and enhancing the reproducibility and accuracy of their research.

Most cited protocols related to «Cetyl alcohol»

DNA was extracted from dried cassava leaves (20 mg) using the modified cetyl trimethylammonium bromide (CTAB) method [22 ]. Briefly, the leaves were crushed in CTAB buffer using metal beads and incubated at 65°C for 30 min. The homogenized mixture was then added to 700 μl of chloroform:isoamyl alcohol (24:1), and DNA was precipitated using isopropanol alcohol for 3 h. The DNA pellet was washed twice with 70% ethanol and then dried at room temperature for approximately 30 min. The DNA was resuspended in water containing 100 μg/ml RNase (Thermo Fisher Scientific, Waltham, MA, USA) and stored at −20°C. The quality and quantity of the isolated DNA were assessed by agarose gel electrophoresis and spectrophotometry [23 (link)].
To isolate DNA from whiteflies, five adults were randomly selected from among those collected at each location. Genomic DNA was isolated as previously described [24 (link)], with minor modifications. Briefly, each whitefly was crushed in lysis buffer (200 mM NaCl and 200 mM Tris-HCl [pH 8.0]) containing β-mercaptoethanol and proteinase K (10 mg/ml), and the mixture was incubated at 65°C for 90 min. DNA was recovered by centrifugation.
To detect SLCMV, the AV1 gene (encoding coat protein) was amplified from SLCMV-infected cassava leaf samples by PCR using sequence-specific primers (forward: 5′-GTT GAA GGT ACT TAT TCC C-3′ and reverse: 5′-TAT TAA TAC GGT TGT AAA CGC-3′) designed in our laboratory. A partial fragment of the mitochondrial cytochrome oxidase 1 (mtCO1) gene was amplified from whitefly DNA using the primers C1-J-2195 (5′-TTG ATT TTT TGG TCA TCC AGA AGT-3′) and L2-N-3014 (5′-TCC AAT GCA CTA ATC TGC CAT ATT A-3′) [25 (link)], yielding a 1258-bp product.
PCR amplification was performed in a 25-μl reaction volume containing 1× PCR buffer (PCR Biosystems, London, UK), 0.2 μM each of forward and reverse primers, and approximately 50 ng of the DNA template. The thermal cycling conditions for the AV1 gene were as follows: initial denaturation at 94°C for 5 min; 35 cycles of denaturation at 94°C for 40 s, annealing at 55°C for 40 s, and elongation at 72°C for 40 s; and final elongation at 72°C for 5 min. For the mtCO1 gene, the thermal cycling conditions were as follows: initial denaturation at 94°C for 5 min; 35 cycles of denaturation at 94°C for 40 s, annealing at 52°C (mtCO1) for 40 s, and elongation at 72°C for 40 s; and final elongation at 72°C for 5 min.
The amplified PCR products were separated on a 1% agarose gel alongside a 1-kb DNA ladder (Thermo Fisher Scientific) that was stained with RedSafe Nucleic Acid Staining Solution (iNtRON Biotechnology, Sangdaewon, South Korea) in 1× Tris–acetate–EDTA buffer. The gels were visualized using a Gel Doc imaging system (Syngene, Frederick, MD, USA). Confirmed negative and positive controls were included in all assays.
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Publication 2021
To examine the differential expression of disease-responsive genes, total RNA from elm stem samples was extracted using a modified CTAB protocol64 (link). Briefly, 10 ml of CTAB extraction buffer (2% (w/v) CTAB (cetyl trimethylammonium bromide), 2% (w/v) PVP (polyvinylpyrrolidone, mol wt 40,000), 100 mM Tris-HCl (pH 8.0), 25 mM EDTA, 2 M NaCl, 0.05% spermidine trihydrochloride) was placed in sterile 50 ml conical centrifuge tubes and preheated to 65 oC in a water bath for 30 min. Woody stem samples were ground in liquid nitrogen using IKA® A11 Basic Analytical Mill (IKA, Wilmington, USA), and further ground using a mortar and pestle. About 2 g of the ground tissues was quickly transferred to the warm extraction buffer after the addition of 2% of β-mercaptoethanol to the buffer. Tubes were vortexed vigorously for 2 min and then incubated at 65 °C for 45 min. Samples were vortexed every 15 min during incubation. Ten milliliter of chloroform-isoamyl alcohol (24:1) (Sigma-Aldrich Co., St Louis, USA) was added to each tube followed by vigorous vortexing for 2 min. The components of each tube were transferred to a clean Corex tube. Samples were then centrifuged at 26,000 × g for 10 min at 4 °C using Sorvall RC 6 plus (Thermo Electron Corporation, USA). The aqueous supernatant was transferred to a clean Corex tube and 1/3 volume of 8 M LiCl (Sigma) was added to the supernatant. Tubes were mixed well by shaking and then incubated for 2 h at −20 °C. The total RNA was pelleted by centrifugation at 26,000 × g for 30 min at 4 °C. The supernatant was discarded and the pellet was washed with 1 ml of 80% ethanol and centrifuged again at 26,000 × g for 10 min at 4 °C. The supernatant was removed and the pellet was dried at room temperature. The RNA pellet was dissolved in sterile Milli-Q water. The RNA quantity and quality was estimated using a Synergy H1 hybrid reader (BioTek, Oakville, ON, Canada) and 1.2% agarose gel containing 3% formaldehyde, respectively. All RNA extracts were treated with DNase using the RNase-free DNase set (Qiagen, Mississauga, ON) and then purified using RNeasy Mini Kit (Qiagen). The cDNA was synthesized from 2.5 μg of DNase treated RNA using the SuperScript® VILO™ cDNA Synthesis Kit (Invitrogen, Burlington, ON) in a total volume of 20 μl. Quantitative real time-PCR (qRT-PCR) was performed using CFX connect real-time Detection System (Bio-Rad, Mississauga, ON) and SsoFast™ EvaGreen® Supermix (Bio-Rad) using gene-specific primers (Supplementary Table 1). Results were statistically analyzed using the CFX manager software (Bio-Rad), which was also used to generate the Volcano plots and the clustergram.
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Publication 2016
2-Mercaptoethanol Anabolism Bath Buffers Centrifugation Cetrimonium Bromide Chloroform Deoxyribonucleases DNA, Complementary Edetic Acid Electrons Endoribonucleases Ethanol Formaldehyde Gene Expression Genes Hybrids isopentyl alcohol Nitrogen Oligonucleotide Primers Povidone Real-Time Polymerase Chain Reaction Sepharose Sodium Chloride Spermidine Stem, Plant Sterility, Reproductive Tissues Tromethamine
RNA was extracted using the Spectrum Plant Total RNA Kit (Sigma) with slight modification. To approximately 50 mg frozen sample, 850 μl of CTAB (cetyl trimethylammonium bromide) buffer (pre-warmed at 65°C) and 10 μl of β-mercaptoethanol (Sigma) was added, vortexed and incubated at 65°C for 5 min. To the same tube 600 μl of chloroform:isoamyl alcohol (24:1 v:v) was added and re-extracted. After centrifugation, the supernatant was passed through filter column (Sigma). After diluting the filtrate with 500 μl of 95% EtOH, the combined liquid was passed through binding column. The binding column was then washed with Wash 1 solution and on-column DNAse digestion was carried out for 20 min as per the manufactures instructions (Sigma). The binding column was washed with wash buffers and RNA was eluted using 50–100 μl elution buffer. After quantifying RNA using NanoDrop, cDNA was prepared from 1500 ng total RNA. qRT-PCR was performed using 3 ng RNA equivalent cDNA, gene specific primers (200 nM) (primer sequences provided in Additional file 11) [48 (link)] and 1Z SYBR green (Bio-Rad life sciences, Hercules, CA) on the StepOnePlusTM Real-Time PCR system (Applied Biosystems, Grand Island, NY). Gene expression was calculated using ΔcT or ΔΔcT methods [49 (link)] based on the threshold cycle (cT) values of the target gene and the housekeeping genes, Ubiquitin-conjugating enzyme E2 (Potri.006G205700) and 18S ribosomal RNA (AF206999) (Additional file 11).
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Publication 2014
2-Mercaptoethanol Buffers Centrifugation Cetrimonium Bromide Chloroform Deoxyribonuclease I Digestion DNA, Complementary Ethanol Freezing Gene Expression Genes Genes, Housekeeping isopentyl alcohol Oligonucleotide Primers RNA, Plant RNA, Ribosomal, 18S SYBR Green I Ubiquitin-Conjugating Enzymes
Total microbial genomic DNA, including bacterial and fungal genomic DNA in the feces of piglets, was extracted using a combined method of cetyl trimethyl ammonium bromide (CTAB) and bead-beating. Briefly, 0.25–0.30 g frozen feces were re-suspended in 1.5 ml ice-cold PBS and then were centrifuged at 9000 rpm for 10 min at 4°C to obtain microbial pellets. The pellets were washed in ice-cold PBS repeatedly until the supernatant became clear. Subsequently, the microbial pellets were re-suspended in 800 μl CTAB buffer containing 50 mM CTAB, 1.4 M NaCl, 100 mM Tris-HCl, 20 mM Ethylene Diamine Tetraacetic Acid (EDTA) and then were lysed by beat-beading using FastPrep-24 bead beater (MP Bio) at the top speed for total 240 s with an ice-cold bath for 120 s at the interval. After incubation at 70°C for 20 min, homogenate solution was centrifuged at 10,000 rpm for 10 min to obtain the supernatant. Five microliter of RNAase (10 mg/ml) was added into the supernatant obtained and the solution was incubated at 37°C for 30 min to remove the RNA. After that, three rounds of phenol: chloroform: isoamyl alcohol (V/V/V = 25: 24: 1) extraction were performed. The microbial DNA obtained was precipitated with the solution containing 1.5 ml ice-cold 95% ethanol and 40 μl 3M NaAc (20:1) at −20°C overnight and then re-suspended in 50 ml of Tris-EDTA buffer. Microbial genomic DNA was quantified using Qubit® 3.0 Fluorometer (Life technology) and DNA integrity was determined by gel electrophoresis (concentration of agarose gel: 1%, voltage: 150 V, and electrophoresis time: 40 min). Finally, the DNA samples examined were stored at −80°C until processing.
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Publication 2016
Bacteria Bath Buffers Cetrimonium Bromide Chloroform Cold Temperature DNA, Fungal Edetic Acid Electrophoresis Ethanol Feces Freezing Genome Genome, Microbial isopentyl alcohol Pellets, Drug Phenol Ribonuclease, Pancreatic Sepharose Sodium Chloride Tromethamine
The current methodology comprises two steps: (1) purification of the microbial cells from fecal impurities and (2) lysis of microbial cells to obtain metagenomic DNA with high molecular weight.
At the first step, fresh feces (100 mg) were weighed into a sterile microcentrifuge tube for isolation of purified microbial cells. Microbial cells were sequentially washed with normal saline solution (0.9% NaCl solution) and phosphate-buffered saline (PBS; pH 7.4). The washing steps were optimized for the recovery of a purified bacterial pellet to obtain quality fecal metagenomic DNA for the downstream studies. In 5 sets of replicates, 100 mg of feces were resuspended in 1 ml of normal saline solution by vortexing for 30 s and then centrifuged at ambient room temperature (RT) for 2 min with different speed of 1000 rpm (72 × g), 2000 rpm (287 × g), 3000 rpm (645 × g), 4000 rpm (1147 × g), and 5000 rpm (1792 × g), respectively. The resulting supernatants were subjected to microscopic examination for the presence of fibers and insoluble impurities. Recovered supernatant was centrifuged again at 10,000 rpm (7168 × g) for 1 min at ambient room temperature to collect microbial pellet for downstream processing. Microbial pellet from all replicates was subsequently washed with 1 ml of PBS (pH 7.4) for centrifugation with different speeds as described above. The resulting supernatants were subjected to centrifugation again at 10,000 rpm (7168 × g) for 1 min to recover microbial pellet, which was used for metagenomic DNA isolation at the next step.
At the second step, the purified microbial pellet was resuspended in 500 μl of lysis buffer containing 1% (w/v) cetyl trimethyl ammonium bromide (CTAB), 100 mM of ethylenediaminetetraacetic acid (EDTA), 1.5 M of NaCl, 100 mM of Na3PO4, and 100 mM of Tris–HCl (pH 8.0). After adding 2 μl of proteinase K (20 mg/ml), the mixture was incubated for 10 min at 37 °C with gentle shaking at 100 rpm in orbital shaker incubator. Afterward, sodium dodecyl sulfate (SDS) was added with a final concentration of 1% and the incubation continued for another 20 min at 65 °C with intermittent shaking. The lysate was centrifuged at 13,000 rpm (12,114 × g) for 5 min at ambient room temperature. The resulting supernatant was collected and mixed with an equal volume of saturated phenol:chloroform:isoamyl alcohol (25:24:1), which is then subjected to centrifugation at 10,000 rpm (7168 × g) for 5 min at RT. The aqueous phase was collected and metagenomic DNA was precipitated with 0.6 volume of isopropanol and pelleted by centrifugation at 13,000 rpm (12,114 × g) for 5 min. After washing twice with 70% ethanol, the resulting DNA was dried and finally dissolved into a 50 μl of 1 × Tris–EDTA buffer (pH 8.0).
The qualitative and quantitative analysis of the metagenomic DNA was performed by agarose gel electrophoresis, restriction endonuclease digestion (Sau3A1), NanoQuant (Tecan Group, Mannedorf, Switzerland) estimation, and Qubit® dsDNA HS Assay Kit (Life Technologies, Carlsbad, CA). Metagenomic DNA recovered from all replicates was compared for qualitative and quantitative parameters to obtain optimized condition for metagenomic DNA isolation from human feces. The optimized method is outlined in Figure 1 and was then used to isolate the metagenomic DNA from 10 one-month-old frozen feces stored at −86 °C and 50 random fecal samples (including both fresh and frozen samples) to evaluate its robustness.
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Publication 2016

Most recents protocols related to «Cetyl alcohol»

AQUA/WATER, CETYL ALCOHOL, GLYCERYL STEARATE, PEG‐75 STEARATE, CETETH‐20, STEARETH‐20, ISODECYL NEOPENTANOATE, ± BENTONITE, SODIUM HYALURONATE, PHENOXYETHANOL, GLYCERIN, DIMETHICONE, FRAGRANCE
Publication 2024
Not available on PMC !
The formulation process involved preparing cream bases through emulsification, combining lipophilic substances with hydrophilic ingredients. Extracts of Lemon, Tomato, and Aloe vera were incorporated into the formulation, along with excipients like cetostearyl alcohol, stearic acid, and carbopol. Excipients such as propylene glycol, methylparaben, and cetyl alcohol were used to control the physicochemical properties of the formulation [6, 7] .
Publication 2024
Melatonin was purchased from Vana Darou Gostar Pharmaceutical Company (Tehran, Iran) and rosuvastatin was purchased from Jalinous Pharmaceutical Company (Tehran, Iran). Stearic acid, cetyl alcohol, mineral oil, glycerin, triethanolamine, methylparaben, and propylparaben were gifted kindly from Haiyan Company (Zhejiang, China).
Publication 2024
The composition of the formulations is shown in Table 9. Glyceryl Stearate (and) Ceteareth-20 (and) Ceteareth-12 (and) Cetearyl Alcohol (and) Cetyl Palmitate (Emulgade® SE-PF, BASF Poland), Cetearyl Isononanoate (Cetiol® SN, BASF, Warszawa, Poland), Octyldodecanol (Eutanol® G, BASF, Poland), Paraffinum Liquidum (Avena, Kraków, Poland), Cetyl Alcohol (Lanette® 16, BASF, Poland), Dimethicone (BASF, Poland) as oil phase, and Glycerin (POCH S.A, Poland), water as aqueous phase were homogenized for 3 min using the Heidolph SilentCrusher M homogenizer (Heidolph Instruments GmbH & Co. KG, Schwabach, Germany) at approximately 15,000 rpm. Then, the emulsion was gently stirred using the blender RW 16 (IKA® Werke GmbH & Co. KG, Staufen im Breisgau, Germany), and the FVE lyophilizates and their mixtures (1:1:1) were added at a concentration of 6%. A preservative of natural origin (sodium benzoate, Pol-Aura, Olsztyn, Poland) was added at 0.5% concentration as the maximum permissible concentration in cosmetics. The emulsion without preservatives and FVEs was a reference sample.
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Publication 2024
The materials used in this research include: avocado seed extract, stearic acid (Brataco chemical), liquid paraffin (Brataco chemical), triethanolamine (Lug chemical), glycerin (Brataco chemical), cetyl alcohol (Merck), methyl paraben (Brataco chemical), rose oil, aquadest, BHT (butylated hydroxytoluene) (samiraschem), ethanol (Brataco), methanol pro analyst (Merck), phytochemical screening reagent.
Publication 2024

Top products related to «Cetyl alcohol»

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Cetyl alcohol is a long-chain fatty alcohol that is commonly used in the manufacturing of various personal care and pharmaceutical products. It is a white, waxy solid that is insoluble in water but soluble in organic solvents. Cetyl alcohol is known for its emulsifying and thickening properties, making it a useful ingredient in formulations such as creams, lotions, and ointments.
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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
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Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.
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Stearic acid is a saturated fatty acid with the chemical formula CH3(CH2)16COOH. It is a white, odorless, and waxy solid at room temperature. Stearic acid is commonly used as a laboratory reagent and has various industrial applications.
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Tween 80 is a non-ionic surfactant and emulsifier. It is a viscous, yellow liquid that is commonly used in laboratory settings to solubilize and stabilize various compounds and formulations.
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The NanoDrop is a spectrophotometer designed for the quantification and analysis of small volume samples. It measures the absorbance of a sample and provides accurate results for DNA, RNA, and protein concentration measurements.
The HCS Studio™ 2.0 software is a comprehensive platform for high-content screening (HCS) analysis. It provides tools for image acquisition, analysis, and data management to support cellular imaging and phenotypic profiling studies.
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
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Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.
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Triethylamine is a clear, colorless liquid used as a laboratory reagent. It is a tertiary amine with the chemical formula (CH3CH2)3N. Triethylamine serves as a base and is commonly employed in organic synthesis reactions.

More about "Cetyl alcohol"

Cetyl alcohol, also known as 1-hexadecanol or palmityl alcohol, is a widely used ingredient in cosmetic and pharmaceutical products.
This saturated fatty alcohol with the chemical formula CH3(CH2)14CH2OH serves as an emollient, emulsifier, and thickening agent, contributing to the texture and stability of various formulations.
Cetyl alcohol is commonly found in a variety of personal care items, such as moisturizers, creams, lotions, shampoos, and other topical preparations.
Its unique properties, including its ability to act as a skin conditioning agent and provide a smooth, silky feel, make it a valuable component in the development of effective and well-tolerated topical products.
Researchers and formulators can leverage PubCompare.ai's AI-driven platform to quickly identify the best protocols and procedures for working with cetyl alcohol, streamlining their experiments and enhancing the reproducibility and accuracy of their research.
The platform helps users locate the most effective products and procedures from literature, pre-prints, and patents, using intelligent comparisons to identify the optimal approaches.
In addition to cetyl alcohol, other related compounds like methanol, sodium hydroxide, stearic acid, and Tween 80 may also be of interest in cosmetic and pharmaceutical research.
Analytical techniques such as NanoDrop and HCS studio™ 2.0 software can be used to characterize and evaluate the properties of these substances.
Solvents like DMSO and hydrochloric acid, as well as reagents like triethylamine, may also play a role in the formulation and analysis of cetyl alcohol-based products.
By leveraging the insights and capabilities offered by PubCompare.ai, researchers and formulators can optimize their cetyl alcohol-related experiments, leading to more reproducible and accurate results that drive the development of innovative and effective topical preparations.