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Lucite

Lucite, a transparent thermoplastic polymer, is widely used in various applications due to its durability, versatility, and optical clarity.
It is commonly employed in the production of eyeglasses, signage, and medical devices.
Lucite's unique properties make it an important material in the field of scientific research, particularly in the area of Lucite research protocols.
PubCompare.ai's intelligent comparison tools can help streamline and optimize Lucite research workflows by locating the best protocols from literature, preprints, and patents, while providing data-driven insights to enhance the accuracy and reproducibility of Lucite-based experiments.
Experiece the power of AI-powered protocol discovery and selection today to take your Lucite research to the next level.

Most cited protocols related to «Lucite»

Partial-Body Irradiation Exposure Methodology for Non-Human Primates

Approximately 18 h prior to exposure, food was removed from each animal’s cage to minimize the occurrence of radiation-induced emesis. On the day of irradiation, NHPs were administered an antiemetic, Zofran (Glaxo-SmithKline, 5 Moore Dr., Research Triangle Park, Durham, NC 27713 USA) or Ondansetron, (Hospira, Inc., 275 North Field Dr., Lake Forest, IL 60045 USA) (1–2 mg kg⁻¹ IV or IM), 45–90 min prior to exposure. Anesthetized NHPs were placed in a Plexiglas supine restraint device, and then transported from the NHP housing area to the linear accelerator (LINAC) facility at the University of Maryland, Department of Radiation Oncology.
The NHPs were administered xylazine to ensure proper radiation field placement would be maintained and then exposed to PBI/BM5 with 6MV LINAC-derived photons. PBI was delivered at an approximate dose rate of 0.80 Gy min⁻¹. The MLTD doses delivered included 9.0 Gy, 10.0 Gy, 11.0 Gy, 11.5 Gy, 12.0 Gy, and 12.5 Gy. Animals were positioned such that their tibiae were outside of the beam field. Animals were observed via in-room cameras throughout the exposure procedure. Following PBI, NHPs were anesthetized, transported back to the NHP housing area, administered Lactated Ringer’s Solution (LRS) (10–15 mL kg⁻¹ IV) and a second dose of Zofran or Ondansetron (1–2 mg kg⁻¹ IM or IV), and returned to their home cage.
Prior to beginning the study, the LINAC was calibrated in the geometry used for NHP irradiation. Depth dose measurements were made at the center of a cylindrical phantom that approximates the mean diameter of the experimental rhesus macaque. Three phantoms of increasing size were available. The phantom is made of 0.32-cm Lucite and filled with water. Dose measurements were performed with ion chambers. Absorbed dose to water per unit machine monitor unit (MU) was obtained. The calibration was then transferred to a constancy system for daily quality assurance purposes.
During NHP irradiations, in vivo dosimetry was performed using a silicon diode system. One diode was placed on the chest to measure the delivered dose, and another was secured on the tibiae to confirm that the tissue outside of the beam field was spared irradiation. Quality assurance and control procedures that were performed prior to each set of NHP irradiations included LINAC energy output checks and diode calibrations.

MacVittie T.J., Bennett A., Booth C., Garofalo M., Tudor G., Ward A., Shea-Donohue T., Gelfond D., McFarland E., Jackson W I.I.I., Lu W, & Farese A.M. (2012). THE PROLONGED GASTROINTESTINAL SYNDROME IN RHESUS MACAQUES: THE RELATIONSHIP BETWEEN GASTROINTESTINAL, HEMATOPOIETIC, AND DELAYED MULTI-ORGAN SEQUELAE FOLLOWING ACUTE, POTENTIALLY LETHAL, PARTIAL-BODY IRRADIATION. Health physics, 103(4), 427-453.

Publication 2012

Animals Antiemetics Chest Food Forests In Vivo Dosimetry Lactated Ringer's Solution Linear Accelerators Lucite Macaca mulatta Medical Devices Mucolipidosis Type IV Ondansetron Plexiglas Radiation Radiotherapy Silicon Tibia Tissues Vomiting Xylazine Zofran

Characterizing PET Scanner Spatial Resolution

A 0.3 mm Na-22 point source encapsulated in Lucite with activity of 132
μCi was scanned in an Inveon preclinical PET scanner
(Siemens, Knoxville, TN) at (−15 mm,−15 mm) in the central axial
plane as shown in figure 1(a). The crystal
size of the Inveon scanner is about 1.59 mm and the 2D sinogram size is 128
(radialbins) × 160 (angles). The total number of events collected in the
2D sinogram was 73 312 and the estimated random fraction was less than 0.1%.
To evaluate the effect of point source contrast on measured spatial
resolution, we simulated the background sinogram by forward projecting a uniform
background with different activity concentrations using the system matrix, and
then superimposed the background projection data onto the point source data
before reconstruction. Since the purpose of adding the background is to reduce
the nonlinear response and the effect of non-negativity constraint, there is no
need to introduce Poisson noise in the background data. Maximum-likelihood image
reconstruction was then performed using 5000 iterations of the preconditioned
conjugate gradient (PCG) algorithm (Qi
et al 1998). Only standard normalization was
applied in the reconstruction. The system matrix used during forward projection
and reconstruction includes a geometric projection matrix and a sinogram
blurring matrix (Tohme and Qi 2009 (link)). The
image matrix size was 1005 × 1005 and the pixel size was 0.1 mm. A small
pixel size was used to increase the accuracy of FWHM measurements.
Reconstruction of the background sinogram was also performed, and was subtracted
from the result of the combined reconstruction to produce the point source only
image. Profiles were drawn through the peak of the point source and linear
interpolation was performed between neighboring pixels to measure the FWHM along
the radial and tangential directions. No curve fitting was performed because the
pixel size was much smaller than the expected FWHM. Negative values were not
considered in the FWHM calculation. In our implementation, the point source
contrast was defined as

Contrast = \frac{Reconstructed point peak intensity}{Background intensity} - 1

Here the reconstructed point peak intensity refers to the peak
intensity of the point source in the reconstructed image before subtracting the
background. For comparison, 2D FBP reconstruction using the standard software on
the scanner was performed with a pixel size of 0.097 mm. To show the 2D results
presented here are applicable to other conditions, fully 3D maximum-likelihood
expectation-maximization (MLEM) reconstruction of a point source at the center
of the FOV was also performed with different background values. The image matrix
size in the 3D reconstruction was 256 × 256 × 161 with a voxel
size of 0.194 × 0.194 × 0.796 mm³.

Gong K., Cherry S.R, & Qi J. (2016). On the assessment of spatial resolution of PET systems with iterative image reconstruction. Physics in medicine and biology, 61(5), N193-N202.

Publication 2016

Lucite Reconstructive Surgical Procedures

Comparative Radiosensitivity of CHO Cells

Exponentially growing cells were exposed to radiation at room temperature. Gamma-ray irradiations (LET 0.3 keV/µm) were carried out at a dose rate of 2.5 Gy/min using a Model Mark I-68A nominal 222TBq (6,000 Ci) ¹³⁷Cesium sealed source (J.L. Shepherd, Carlsbad, CA). Hadron radiation experiments were carried out at the National Institute of Radiological Sciences (NIRS) in Chiba, Japan [19 (link)]. Protons were accelerated to 70 MeV (LET 1.1 keV/µm) using the NIRS-930 cyclotron at NIRS [20 (link)]. Carbon-ions and iron-ions were accelerated to 290 MeV/n and 500 MeV/n, respectively, using the heavy ion medical accelerator (HIMAC) at NIRS. The LET of the entrance region for monoenergetic carbon ions and monoenergetic iron ions were 13 and 200 keV/μm, respectively. Monoenergetic carbon ions with a LET of 70 keV/μm were obtained by Lucite attenuation. The dose-averaged LET of the carbon ions at the middle of the 6-cm spread-out Bragg peak (SOBP) is ∼50 keV/µm at a distance of 119 mm from the entrance [21 (link)]. Dose rates for carbon-ions, iron-ions and protons were set at 3 Gy/min. All irradiation was carried out at room temperature. LET values of the various radiation types are summarized in Table 1.

Table 1.

Ratios of D₁₀ doses for CHO wild-type and xrs5 cells exposed to various radiations

Radiation	LET keV/μm	(A) D₁₀ of CHO10B2	(B) D₁₀ of xrs5	Ratio (A)/(B)
Gamma rays	0.3	6.37 Gy	1.18 Gy	5.40
Proton Mono	1.1	5.31Gy	1.16 Gy	4.58
Carbon Mono	13	3.79 Gy	0.91 Gy	4.16
Carbon SOBP	50	3.16 Gy	1.05 Gy	3.01
Carbon Mono	70	2.49 Gy	0.94 Gy	2.65
Iron Mono	200	1.89 Gy	1.00 Gy	1.89
⁶⁴Cu-ATSM	NA	0.97 Bq/cell	0.40 Bq/cell	2.43

McMillan D.D., Maeda J., Bell J.J., Genet M.D., Phoonswadi G., Mann K.A., Kraft S.L., Kitamura H., Fujimori A., Yoshii Y., Furukawa T., Fujibayashi Y, & Kato T.A. (2015). Validation of 64Cu-ATSM damaging DNA via high-LET Auger electron emission. Journal of Radiation Research, 56(5), 784-791.

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Publication 2015

Carbon Cells Cyclotrons Gamma Rays Heavy Ions Ions Iron Lucite Protons Radiation Radiation Exposure Radiotherapy X-Rays, Diagnostic

Portable XRF for In Vivo Bone Lead Measurement

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Publication 2017

Bones Cadaver Diaphyses Filtration Homo sapiens Human Body Iron Lucite Medical Devices Plaster of Paris Radiography Radioisotopes Silicon Silver Skin Tibia Tissues

Cellular Radiation and Chemical Response

Cells were irradiated with TITAN x-ray irradiator with 200 kVp, 20 mA, 0.5 cm of Al and Cu filter (Shimadzu, Japan). Heavy ion treatment was performed by HIMAC (Heavy Ion Medical Accelerator in Chiba). The accelerated ions used in this study were carbon ions (290 MeV/n), neon (400 MeV/n), silicon (490 MeV/n), argon (500 MeV/n), and iron ions (500 MeV/n). The details concerning the beam characteristics of carbon-ion beams, biological irradiation procedures, and dosimetry have been described elsewhere [19 (link),20 (link)]. We used several kinds of beams having different LET values, using Lucite absorbers with various thicknesses to change the energy of the beams. At the sample position, we estimated the LET values of carbon (13, 30, 50, 70 keV/μm), neon (31, 70, 120 keV/μm), silicon (55, 150, 250 keV/μm), argon (100 keV/μm), and iron (200 keV/μm). Taking fragmentations into consideration, dose was calculated from fluence [21 (link)-23 ]. Asynchronously dividing cells cultured in T12.5 flasks were irradiated at room temperature. For chemical treatment, cycling cells in T12.5 culture flasks were exposed to series of concentration of bleocin, a single component of bleomycin family group A (Calbiochem, Japan), which induces DNA strand breaks, camptothecin (CPT, Sigma, Japan) which is a Topoisomerase I inhibitor, mitomycin C (MMC, Funakoshi, Japan) which induces DNA crosslink, or cisplatin (Nippon Kayaku, Japan) which induces DNA crosslink for 1 hour at 37°C.
After exposure to ionizing radiation or chemical treatment, cells were trypsinized and re-plated in P-100 cell culture dishes. HeLa and U87-MG cells were cultured for 10 to 14 days, and U-CH1-N cells were kept in an incubator for 3 to 4 weeks. Plating efficiency of U-CH1-N, U87-MG, and HeLa cells were 4.8%, 32%, and 70%, respectively. After colonies were formed, cells were fixed with 100% ethanol and stained with crystal violet solution. Colonies were observed under microscope and colonies containing more than 50 cells were counted as survivors. Cell survival assay was carried out 2 to 4 times independently. Radiation exposed cell survival curves were fitted with linear quadratic model by PRISM5 software on MacOSX10.6. Error bars indicate standard error of the means.

Kato T.A., Tsuda A., Uesaka M., Fujimori A., Kamada T., Tsujii H, & Okayasu R. (2011). In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs. Radiation Oncology (London, England), 6, 116.

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Publication 2011

Argon Biological Assay Biopharmaceuticals Bleomycin Camptothecin Carbon Carbon-13 Cell Culture Techniques Cells Cell Survival Cisplatin DNA Breaks Electromagnetic Radiation Ethanol Heavy Ions HeLa Cells Hyperostosis, Diffuse Idiopathic Skeletal Iron Lucite Microscopy Neon Radiation Exposure Radiometry Silicon Survivors Topoisomerase I Inhibitors Violet, Gentian X-Rays, Diagnostic

Most recents protocols related to «Lucite»

Mössbauer Spectroscopy of Historical Tiles

The analyzed samples were homogeneous powder gently scraped from the surfaces and interfaces of the stained specimens: three tiles of Carrara and Travertine. Two samples were similarly collected from indoor specimens and from the archeological artifacts, observing the BB.CC. regulations for the sampling modality.
The absorbers were prepared by pressing finely the powder samples mixed with powder acrylic resin (Lucite) to self-supporting disks of about 10 mm diameter. Sample quantity at this stage was strictly dependent on the availability of the examined material, with the drawback of—in some cases—staying at or near the detection limit. The amount used should correspond to about 2 mg of iron oxide, with an absorption density in which the thickness does not affect the Mössbauer results. In our case, these concentrations were very difficult to reach, and to solve this problem long acquisition times (one or two weeks) were used to ensure a good signal/noise ratio in data reading, and in all cases the powders collected at the surface and interface zones were mixed and analyzed as a single sample.
Spectra were collected at 298 K (room temperature, RT) using a ⁵⁷Co/Rh source and a conventional constant acceleration mode. A multichannel analyzer with 512 channels was used for the data recording at a range of velocity of −10 to +10 mm/s. A highly pure sample of α-iron was used to calibrate the speed, and raw data were collected in 512 channels. Spectra were elaborated by Recoil 1.04 [44 ] software accounting for symmetric Lorentz curves. The χ² test was used to individuate the best conditions and uncertainty was obtained by the covariance matrix. A 0.02 mm/s uncertainty resulted for the isomer shift (IS), quadrupole splitting (QS), and magnetic hyperfine field (HF). Uncertainty no lower than ±3% was estimated for the doublet areas.

Reale R., Andreozzi G.B., Sammartino M.P, & Salvi A.M. (2023). Analytical Investigation of Iron-Based Stains on Carbonate Stones: Rust Formation, Diffusion Mechanisms, and Speciation. Molecules, 28(4), 1582.

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Publication 2023

Acceleration Acrylic Resins ferric oxide Iron Isomerism Lucite Magnetic Fields Powder Vitelliform Macular Dystrophy

Polycarbonate Nanotopographical Surfaces

Polycarbonate nanotopographical culture surfaces comprised of 120 nm diameter pits were produced by injection molding using an Engle Victory 28 hydraulic injection moulder. Master SQ (300 nm centre-centre spacing and 100 nm depth arrays) or NSQ (arrays with an additional 50 nm offset spacing of pits) nickel dies were produced from patterned resists of silicon-coated substrates fabricated on silicon-coated 100 nm PMMA (Elvacite 2041, Lucite International) by electron beam lithography. The silicon substrates were exposed to a 50 kV electron beam, developed in 1:3 MIBK:IPA for 30 s, rinsed in IPA and dried in a nitrogen stream. Patterned resists were sputtered coated ith 50 nm Ni-V and electroplated to a thickness of ~300 μm (outsourced to DVDNorden, Denmark). Nickel shims were chloroform cleaned for 10–15 min in an ultrasonic bath, rinsed in acetone and IPA, and dried once more in gaseous nitrogen. Surfaces were produced by injection moulding through heating polycarbonate (Makrolon® OD2015) to 180 °C and applying a clamping force of 250 kN to imprint nanotopographical pattern onto the surface of the polycarbonate. Final dimensions of each substrate being 24 mm × 24 mm. After cooling to 70 °C, separation of the press and polymer surfaces was achieved. Unpatterned (flat) polycarbonate substrates were injection moulded against planar shims and used as controls.

Ross E.A., Turner L.A., Donnelly H., Saeed A., Tsimbouri M.P., Burgess K.V., Blackburn G., Jayawarna V., Xiao Y., Oliva M.A., Willis J., Bansal J., Reynolds P., Wells J.A., Mountford J., Vassalli M., Gadegaard N., Oreffo R.O., Salmeron-Sanchez M, & Dalby M.J. (2023). Nanotopography reveals metabolites that maintain the immunomodulatory phenotype of mesenchymal stromal cells. Nature Communications, 14, 753.

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Publication 2023

Acetone Bath Chloroform Electrons Gases Imprinting (Psychology) Lucite Nickel Nitrogen polycarbonate Polymers Polymethyl Methacrylate SHIMS Silicon Ultrasonics Van der Woude syndrome

Palynological Analysis of Bahariya Formation

Fifty-six subsurface core and cuttings samples belonging to the early Cenomanian Bahariya Formation, gathered from four wells in the north Western Desert, Egypt, are palynologically investigated for this study. Such subsurface material is available, as the Bahariya Formation is an important target for petroleum exploration in the north Western Desert. The studied wells are GPJ-1, TSW-21, GPT-3, and GPTSW-7 wells (Fig 3). The Bahariya Formation in the GPTSW-7 and GPT-3 wells, was previously examined for its palynostratigraphy and palynofacies analysis ([62 ,72 ,73 ], respectively). However, the other two wells are investigated here for the first time and their results were supplemented with a re-interpretation of the data retrieved from both GPTSW-7 and GPT-3 boreholes, as outlined in Table 1.
Samples were processed following maceration techniques including cold hydrochloric and hydrofluoric acid (HCl-HF) demineralization but lacking alkali or oxidation handlings (e.g., [78 ]). The neutralized residues were sieved at 10 μm using a nylon mesh, stained with Safranin ‘O’, and strew-mounted on slides with the aid of Lucite International’s Elvacite 2044 acrylic resin. When drying, this offers a permanent mount with good optic properties, and notably for photography, the palynomorphs are all held to the surface of the coverslip and close to a single plane of focus. Relevant slides and residues are stored at the Geology Department, Faculty of Science, Mansoura University, Mansoura, Egypt.

El Atfy H., Coiffard C., El Beialy S.Y, & Uhl D. (2023). Vegetation and climate change at the southern margin of the Neo-Tethys during the Cenomanian (Late Cretaceous): Evidence from Egypt. PLOS ONE, 18(1), e0281008.

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Publication 2023

Acrylic Resins Alkalies ARID1A protein, human Cold Temperature Eye Faculty Hydrochloric acid Hydrofluoric acid Lucite Nylons Petroleum safranine T

Chronic Cystitis-Induced Colorectal Hypersensitivity

Rats were i.p. injected with CPM (MilliporeSigma, C0768, 50 mg/kg) every 3 days to induce chronic cystitis. Control rats received saline injections. At day 17, colorectal sensitivity was measured by recording the response to CRD as described in detail previously (59 (link)). Briefly, all animals were habituated to the test environment for 3 days before measurement. On the day of the behavior test, rats were lightly anesthetized with isoflurane. A plastic balloon attached to tygon tubing was inserted 6 cm into the colorectum via the anus and fixed by taping the tubing to the tail. Rats were placed in small lucite cubicles (20 × 8 × 8 cm) and allowed to adapt for 30 minutes. CRD was performed by rapidly inflating the balloon using a sphygmomanometer. The balloon was inflated to various pressures (10, 20, 40, 60, 80, and 100 mmHg) for 20 seconds, followed by a 4-minute rest. Abdominal withdrawal reflex (AWR) responses to each CRD were observed by an operator blinded to the distension pressure and treatment (saline, CPM, CPM plus G15, or CPM plus vehicle). The AWR was scored as follows: 0 = normal behavior; 1 = slight head movement without an abdominal response; 2 = contraction of the abdominal muscles; 3 = lifting of the abdominal wall; or 4 = body arching and lifting of the pelvis. Each measurement was performed 3 times, and the repetitive AWR scores for each distension pressure were averaged.
In order to study the effects of GPER blockade on CPM-induced colorectal hypersensitivity, the GPER antagonist G15 (1 mM at the rate of 0.5 μL/h) was chronically infused intracisternally. In brief, after the dura mater between the foramen magnum and C1 lamina was perforated with a syringe needle, a PE-10 catheter was advanced 2 mm into the cisterna magna. The catheter was sealed to the dura with tissue glue, and the incision was closed with layered sutures. The outer end of the catheter was connected to an osmotic minipump (Alzet 2004), which was placed under the skin in the neck region. The control group received a continuous infusion of aCSF.

Jiao Y., Gao P., Dong L., Ding X., Meng Y., Qian J., Gao T., Wang R., Jiang T., Zhang Y., Kong D., Wu Y., Chen S., Xu S., Tang D., Luo P., Wu M., Meng L., Wen D., Wu C., Zhang G., Shi X., Yu W, & Rong W. (2023). Molecular identification of bulbospinal ON neurons by GPER, which drives pain and morphine tolerance. The Journal of Clinical Investigation, 133(1), e154588.

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Publication 2023

Abdomen Animals Anus Behavior Test Catheters Cystitis Dura Mater GPER protein, human Head Movements Human Body Hypersensitivity Isoflurane Lucite Magna, Cisterna Magnums, Foramen Muscle Contraction Neck Needles Neoplasm Metastasis Osmosis Pelvis Pressure Rattus norvegicus Reflex, Abdominal Saline Solution Skin Sphygmomanometers Sutures Syringes Tail Tissues Wall, Abdominal

Tail Flick Reflex Latency Test

Mice were lightly restrained in small lucite cubicles. One-third of the mouse’s tail was dipped into a water bath with a temperature of 48.0°C. The latency to flick or curl the tail was recorded. A maximum cutoff of 15 seconds was set to avoid tissue injury. The investigator was blinded to the treatments and genotypes.

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Publication 2023

Bath Endocytic Vesicles Genotype Injuries Lucite Mus Neoplasm Metastasis Tail Tissues

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More about "Lucite"

Lucite, a clear and durable thermoplastic polymer, is widely used in various applications due to its versatility and optical clarity.
It is commonly employed in the production of eyeglasses, signage, and medical devices, as well as in scientific research.
Methacrylate, a closely related term, is the key monomer used to produce Lucite.
Polymethyl methacrylate (PMMA), another name for Lucite, is a crucial material in the field of Lucite research protocols.
PubCompare.ai's intelligent comparison tools can help streamline and optimize Lucite/PMMA research workflows by locating the best protocols from literature, preprints, and patents, while providing data-driven insights to enhance the accuracy and reproducibility of Lucite-based experiments.
Teklad lab chow and Sprague-Dawley rats are often used in Lucite research, while Ussing chambers and Gammacell 40 Exactor may be employed to study the material's properties.
N,N-dimethyl-p-toluidine and the NI USB-6211 Data Acquisition Board are also relevant to Lucite research.
Experienence the power of AI-powered protocol discovery and selection today to take your Lucite/PMMA research to the next level.