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Eosin

Eosin, a red dye derived from fluorescein, is a widely used histological stain that highlights acidic cellular components.
It provides a vivid, contrasting counterpart to basic dyes like hematoxylin, enabling researchers to visualize and differentiate tissue structures.
Eosin is commonly utilized in the eosin-hematoxylin (H&E) staining procedure, a standard technique for examining cell morphology and identifying pathological changes.
Its ability to selectively bind to proteins, nucleic acids, and other acidic biomolecules makes eosin an indispensable tool in the fields of histology, cytology, and pathology.
Reseachers can expore a vast databse of eosin-related literature, pre-prints, and patents using PubCompare.ai's AI-driven platform, which helps identify the most reproducible and acurate eosin protocols and products to enhance their research.

Most cited protocols related to «Eosin»

1
Integrating Multimodal Data for Breast Cancer Subtyping
Information on lymph node status, stage and tumour size was available from original histopathology reports for all studies. Expert breast cancer pathologists reviewed FFPE sections stained with haematoxylin and eosin (H&E) from tumours with available material and scored histological tumour type, grade, tumour cellularity and lymphocytic infiltration.
Immunohistochemistry-based (IHC) scoring of ER status was, where available, used to classify ER−positive (ER+) and ER−negative (ER−) tumours. To confirm this classification for samples which had gene expression data available, we fit a two-component Gaussian mixture model to the expression levels of ESR1 using the mixtools package59 in R, and computed the probabilities of the samples belonging to the two distributions defined by the components. The distribution yielding the higher probability was selected to represent the ER status for each sample. Where the calls between the two systems differed, we used the expression-derived classification if the probability of belonging to the opposite distribution was at least 5 × higher than for the distribution described by IHC; this scheme was chosen so as to assign more weight to the IHC classification, as this is currently the clinical gold standard. We performed a similar analysis with ERBB2 expression levels to corroborate the IHC-based HER2 calls. For patients without expression data (n=416), we used the IHC scores to assign ER and HER2 status. Similarly, gene expression-based classification was used for samples without IHC data.
Pereira B., Chin S.F., Rueda O.M., Vollan H.K., Provenzano E., Bardwell H.A., Pugh M., Jones L., Russell R., Sammut S.J., Tsui D.W., Liu B., Dawson S.J., Abraham J., Northen H., Peden J.F., Mukherjee A., Turashvili G., Green A.R., McKinney S., Oloumi A., Shah S., Rosenfeld N., Murphy L., Bentley D.R., Ellis I.O., Purushotham A., Pinder S.E., Børresen-Dale A.L., Earl H.M., Pharoah P.D., Ross M.T., Aparicio S, & Caldas C. (2016). The somatic mutation profiles of 2,433 breast cancers refines their genomic and transcriptomic landscapes. Nature Communications, 7, 11479.
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Publication 2016
Breast Carcinoma Cells Eosin erbb2 Gene Gene Expression Gold Immunohistochemistry Lymphocyte Neoplasms Nodes, Lymph Pathologists Patients
2
Neuropathological Examination of Aging Brains
Brain autopsies were conducted at pre-determined sites across the US, with a mean postmortem interval of 8.3 (SD=8.0) hours. The cerebellar and cerebral hemispheres were cut coronally into 1cm slabs. Slabs not designated for freezing were fixed for at least 48–72 hours. Neuropathologic evaluations were performed at the Rush, blinded to clinical data, and reviewed by a board-certified neuropathologist, as reported elsewhere.16 (link),17 (link) A uniform examination included assessment for common vascular and neurodegenerative conditions in aging. Examination for cerebral infarcts documented age (acute/subacute/chronic), size, and location (side and region) of infarcts visible to the naked eye on fixed slabs.17 (link) All grossly visualized and suspected macroscopic infarcts were dissected for histologic confirmation. For analyses, chronic macroscopic infarcts were characterized as present or absent.17 (link)
A minimum of nine regions in one hemisphere were examined for microinfarcts on 6µm paraffin-embedded sections, stained with hematoxylin/eosin. We examined six cortical regions (midfrontal, middle temporal, entorhinal, hippocampal, inferior parietal, and anterior cingulate cortices), two subcortical regions (anterior basal ganglia, thalamus), and midbrain. Locations of microinfarcts were recorded. Because acute and subacute microinfarcts were unlikely to be related to dementia, we only considered chronic microinfarcts for this study. These included cavitated or incomplete infarcts, with few remaining macrophages and fibrillary gliosis. In primary analyses, each case was classified according to whether any chronic microinfarct was present. We created additional variables for secondary analyses. For quantity, we created a predictor with three levels: no (reference level), one, and multiple microinfarcts. For location, we created two variables: cortical (presence of any microinfarcts in any cortical region; reference = no cortical microinfarcts) and subcortical microinfarcts (presence of any microinfarcts in any subcortical region; reference = no subcortical microinfarcts). To investigate quantity and location simultaneously, we created four variables: one cortical microinfarct and multiple cortical microinfarcts (compared to no cortical microinfarcts), and one subcortical microinfarct and multiple subcortical microinfarcts (compared to no subcortical microinfarcts).
Each brain was examined for pathological markers of other common neurodegenerative conditions associated with dementia. AD pathology was assessed in fixed tissue which was paraffin embedded, cut into 6µm sections, and mounted on slides.17 (link) Using a modified Bielschowsky silver stain, we counted neuritic plaques, diffuse plaques, and neurofibrillary tangles. Counts were scaled separately in each region and averaged across regions to create summary scores of each markers for each subject. For analyses, we created a summary score of global AD pathology, by averaging the summary scores of the three markers.16 (link) Lewy body pathology was identified in 6µm sections of cortex and substantia nigra, using α-synuclein immunohistochemistry (Zymed, 1:100).18 (link) For analyses, Lewy body data was dichotomized as present (if identified in any brain region) vs. absent.
Arvanitakis Z., Leurgans S.E., Barnes L.L., Bennett D.A, & Schneider J.A. (2011). Microinfarct Pathology, Dementia, and Cognitive Systems. Stroke; a journal of cerebral circulation, 42(3), 722-727.
Publication 2011
alpha-Synuclein Autopsy Basal Ganglia Blood Vessel Brain Cerebellum Cerebral Hemispheres Cerebral Infarction Cortex, Cerebral Dementia Eosin Gliosis Gyrus, Anterior Cingulate Immunohistochemistry Infarction Lewy Bodies Macrophage Mesencephalon Neurodegenerative Disorders Neurofibrillary Tangle Neuropathologist Paraffin Paraffin Embedding Pathologic Processes Senile Plaques Silver Stains Substantia Nigra Thalamus
3
Shallow Whole-Genome Sequencing Pipeline
Fifteen LGGs (van Thuijl et al. 2014 (link)), two SCCs (Bhattacharya et al. 2011 (link)), and the breast cancer cell line BT474 were used to develop and illustrate the shallow WGS pipeline presented. All material used from LGG and SCC tumors was derived from FFPE archival samples. Patient consent was obtained for SCCs as published previously (Bhattacharya et al. 2011 (link)). LGG samples were collected from five Dutch hospitals (VU University Medical Center in Amsterdam, Academic Medical Center in Amsterdam, Radboud University Medical Center in Nijmegen, St. Elisabeth Hospital in Tilburg, and Isala Klinieken in Zwolle). Sample collection was approved by the Medical Ethics Committees of all five hospitals. Areas containing > 60% tumor cells were outlined on hematoxylin and eosin-stained slides, and 10 subsequent 10-μm sections were used for DNA isolations.
DNA from the LGG samples was isolated as previously described (van Essen and Ylstra 2012 (link)). DNA concentrations were measured with the Nanodrop 2000 (Fisher Scientific), and 500 ng was used as input for Shallow WGS laboratory preparation. DNA from the SCC samples was isolated as previously described (Bhattacharya et al. 2011 (link)), DNA concentrations were measured with the Qubit 2.0 fluorometer dsDNA BR Assay (Life Technologies), and 250 ng DNA used as input for shallow WGS laboratory preparation. The BT474 breast tumor cell line was cultured and DNA isolated as previously described (Krijgsman et al. 2013 ). DNA concentration was measured with the Qubit fluorometer and 250 ng used as input.
Scheinin I., Sie D., Bengtsson H., van de Wiel M.A., Olshen A.B., van Thuijl H.F., van Essen H.F., Eijk P.P., Rustenburg F., Meijer G.A., Reijneveld J.C., Wesseling P., Pinkel D., Albertson D.G, & Ylstra B. (2014). DNA copy number analysis of fresh and formalin-fixed specimens by shallow whole-genome sequencing with identification and exclusion of problematic regions in the genome assembly. Genome Research, 24(12), 2022-2032.
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Publication 2014
Biological Assay Breast Cell Line, Tumor Cells DNA, Double-Stranded Eosin Ethics Committees, Clinical Hematoxylin isolation MCF-7 Cells Neoplasms Patients Specimen Collection
4
Comprehensive Cancer Genomic Profiling
CGP was performed using the FoundationOne assay (Cambridge, MA, USA), as previously described in detail [55 (link), 56 (link)]. Briefly, the pathologic diagnosis of each case was confirmed by review of hematoxylin and eosin stained slides and all samples that advanced to DNA extraction contained a minimum of 20% tumor cells. Hybridization capture of exonic regions from 185, 236, 315, or 405 cancer-related genes and select introns from 19, 28, or 31 genes commonly rearranged in cancer was applied to ≥50 ng of DNA extracted from formalin-fixed, paraffin-embedded clinical cancer specimens. These libraries were sequenced to high, uniform median coverage (>500×) and assessed for base substitutions, short insertions and deletions, copy number alterations, and gene fusions/rearrangements [55 (link)]. Data from all versions of the FoundationOne assay were used in the analysis. Hybridization capture baits for PMS2 are identical across all assay versions.
Chalmers Z.R., Connelly C.F., Fabrizio D., Gay L., Ali S.M., Ennis R., Schrock A., Campbell B., Shlien A., Chmielecki J., Huang F., He Y., Sun J., Tabori U., Kennedy M., Lieber D.S., Roels S., White J., Otto G.A., Ross J.S., Garraway L., Miller V.A., Stephens P.J, & Frampton G.M. (2017). Analysis of 100,000 human cancer genomes reveals the landscape of tumor mutational burden. Genome Medicine, 9, 34.
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Publication 2017
Biological Assay Cells Copy Number Polymorphism Crossbreeding Diagnosis Eosin Exons Formalin Gene, Cancer Gene Deletion Gene Rearrangement Genes Hematoxylin Insertion Mutation Introns Malignant Neoplasms Neoplasms Paraffin Embedding PMS2 protein, human
5
Genetic Manipulation of Apoptosis Pathways in Mice
Mice with a deleted allele of caspase-8 were generated by germ line deletion of a previously-described caspase-8flox allele2 (link). RIPK3 deficient animals were obtained from Vishva Dixit21 (link). Genotypes were confirmed by tail snip PCR as previously described. For Jo2 injections, animals were injected via tail vein with 15µg purified Jo2 in LPS-free PBS per animal. Liver enzymes were assayed using a Trilogy Multi-Purpose Analyzer System from Drew Scientific, and liver sections were created and stained with hematoxylin and eosin, in the St Jude Veterinary Pathology Core facility. For SEB injections, 50µg SEB (Toxin Technology Inc.) per animal was injected via tail vein and T-cell populations were monitored by retro-orbital bleed and FACS as detailed previously. The St. Jude Institutional Animal Care and Use Committee approved all procedures in accordance with the Guide for the Care and Use of Animals.
Oberst A., Dillon C.P., Weinlich R., McCormick L.L., Fitzgerald P., Pop C., Hakem R., Salvesen G.S, & Green D.R. (2011). Catalytic activity of the caspase-8-FLIPL complex inhibits RIPK3-dependent necrosis. Nature, 471(7338), 363-367.
Publication 2011
Alleles Animals Caspase Caspase-8 Deletion Mutation Enzymes Eosin Genotype Germ Line Institutional Animal Care and Use Committees Liver Mus Population Group RIPK3 protein, human T-Lymphocyte Tail Toxins, Biological Veins

Most recents protocols related to «Eosin»

1
Histological analysis of traumatic brain injury
Authorizations for reporting these three cases were granted by the Eastern Ontario Regional Forensic Unit and the Laboratoire de Sciences Judiciaires et de Médecine Légale du Québec.
The sampling followed a relatively standardized protocol for all TBI cases: samples were collected from the cortex and underlying white matter of the pre-frontal gyrus, superior and middle frontal gyri, temporal pole, parietal and occipital lobes, deep frontal white matter, hippocampus, anterior and posterior corpus callosum with the cingula, lenticular nucleus, thalamus with the posterior limb of the internal capsule, midbrain, pons, medulla, cerebellar cortex and dentate nucleus. In some cases, gross pathology (e.g. contusions) mandated further sampling along with the dura and spinal cord if available. The number of available sections for these three cases was 26 for case1, and 24 for cases 2 and 3.
For the detection of ballooned neurons, all HE or HPS sections, including contusions, were screened at 200×.
Representative sections were stained with either hematoxylin–eosin (HE) or hematoxylin-phloxin-saffron (HPS). The following histochemical stains were used: iron, Luxol-periodic acid Schiff (Luxol-PAS) and Bielschowsky. The following antibodies were used for immunohistochemistry: glial fibrillary acidic protein (GFAP) (Leica, PA0026,ready to use), CD-68 (Leica, PA0073, ready to use), neurofilament 200 (NF200) (Leica, PA371, ready to use), beta-amyloid precursor-protein (β-APP) (Chemicon/Millipore, MAB348, 1/5000), αB-crystallin (EMD Millipore, MABN2552 1/1000), ubiquitin (Vector, 1/400), β-amyloid (Dako/Agilent, 1/100), tau protein (Thermo/Fisher, MN1020 1/2500), synaptophysin (Dako/Agilent, ready to use), TAR DNA binding protein 43 (TDP-43) ((Protein Tech, 10,782-2AP, 1/50), fused in sarcoma binding protein (FUS) (Protein tech, 60,160–1-1 g, 1/100), and p62 (BD Transduc, 1/25). In our index cases, the following were used for the evaluation of TAI: β-APP, GFAP, CD68 and NF200; for the neurodegenerative changes: αB-crystallin, NF200, ubiquitin, tau protein, synaptophysin, TDP-43, FUS were used.
For the characterization of the ballooned neurons only, two cases of fronto-temporal lobar degeneration, FTLD-Tau, were used as controls. One was a female aged 72 who presented with speech difficulties followed by neurocognitive decline and eye movement abnormalities raising the possibility of Richardson’s disorder. The other was a male aged 67 who presented with a primary non-fluent aphasia progressing to fronto-temporal demαentia. In both cases, the morphological findings were characteristic of a corticobasal degeneration.
Michaud J., Plu I., Parai J., Bourgault A., Tanguay C., Seilhean D, & Woulfe J. (2023). Ballooned neurons in semi-recent severe traumatic brain injury. Acta Neuropathologica Communications, 11, 37.
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Publication 2023
Amyloid beta-Protein Precursor Amyloid Proteins Antibodies Broca Aphasia Cloning Vectors Congenital Abnormality Contusions Corpus Callosum Cortex, Cerebellar Cortex, Cerebral Corticobasal Degeneration Crystallins Dura Mater Eosin Eye Abnormalities Eye Movements Frontotemporal Lobar Degeneration FUBP1 protein, human Glial Fibrillary Acidic Protein Hematoxylin Immunohistochemistry Internal Capsule Iron Males Medial Frontal Gyrus Medulla Oblongata Mesencephalon Movement Movement Disorders neurofilament protein H Neurons Nucleus, Dentate Nucleus, Lenticular Occipital Lobe Periodic Acid phloxine Pons Proteins protein TDP-43, human RNA-Binding Protein FUS Saffron Sarcoma Seahorses Speech Spinal Cord Staining Synaptophysin Temporal Lobe Thalamus Ubiquitin White Matter Woman
2
Histological Examination of Distal Colon
The distal colon specimens were fixed with 4% paraformaldehyde for 24 h and embedded in paraffin. Then, paraffin-embedded colonic tissues were sectioned (4 μm in thickness), stained with hematoxylin and eosin, and analyzed by a pathologist without information on the experimental procedures based on the scoring criteria as described previously [19 (link)].
Yang J., Wang L., Mei M., Guo J., Yang X, & Liu S. (2023). Electroacupuncture repairs intestinal barrier by upregulating CB1 through gut microbiota in DSS-induced acute colitis. Chinese Medicine, 18, 24.
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Publication 2023
Colon Eosin Paraffin Paraffin Embedding paraform Pathologists Tissues
3
Histological Analysis of African and South American Lungfish Olfactory Organs
All procedures were approved by the local Animal Ethics Committee of Iwate University. The African lungfish P. aethiopicus and South American lungfish, L. paradoxa, were purchased from commercial suppliers. The fishes were anesthetized with tricaine methanesulfonate and euthanized by decapitation. Information pertaining to the animals is shown in Table 1. Juvenile and adult individuals of each lungfish were used. According to Mlewa and Green (2004) [29 (link)] and Jorgensen and Joss (2010) [30 ], P. aethiopicus individuals over 43 cm in body length (BL) reach sexual maturity. Thus, P. aethiopicus #1 (BL 50 cm) and L. paradoxa #1 (BL 65 cm) were regarded as adults, whereas P. aethiopicus #2–4 and L. paradoxa #3 (BL 35 cm or less) were regarded as juveniles [29 (link), 30 ]. Also, we confirmed during dissection whether they had functional genital organs or not.

Animals

Animal NoTotal body length (cm)Body weight (g)SexApplication
P. aethiopicus150.0349.0FISH (left)/RNA extraction (right)
235.0150.6MDice CT
331.5100.0unknownISH
434.0118.3FSEM
L. paradoxa165.0994.5FRNA extraction (left)/ISH (right)
318.518.6MISH

ISH in situ hybridization; Dice CT Diffusible iodine-based contrast-enhanced computed tomography; SEM Scanning Electron Microscopy

For histological examination, olfactory organs were dissected from the heads and fixed in 4% paraformaldehyde in 0.1 M phosphate buffer (PB, pH 7.4). The specimens were cryoprotected in a sucrose gradient (10%, 20%, and 30% in 0.1 M PB), embedded in O.C.T. compound (Sakura Finetek, Tokyo, Japan), and sectioned sagittally using a cryostat. Sections (20 µm in thickness) were thaw mounted on MAS-coated slides (Matsunami, Osaka, Japan), air-dried, and processed for hematoxylin–eosin staining, immunohistochemistry, and in situ hybridization.
Nakamuta S., Yamamoto Y., Miyazaki M., Sakuma A., Nikaido M, & Nakamuta N. (2023). Type 1 vomeronasal receptor expression in juvenile and adult lungfish olfactory organ. Zoological Letters, 9, 6.
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Publication 2023
Adult Animal Ethics Committees Animals Body Weight Buffers Decapitation Dissection Electrons Eosin Fishes Genitalia Head Human Body Immunohistochemistry In Situ Hybridization Iodine methanesulfonate Negroid Races paraform Phosphates Sense of Smell Sexual Maturation South American People Sucrose tricaine X-Ray Computed Tomography
4
Tissue Processing and Immunostaining Protocol
Hematoxylin and eosin (HE) and periodic acid Schiff (PAS) staining were performed as follows. The tissues were fixed in 4% paraformaldehyde and subsequently embedded in paraffin. Then, 4-μm-thick cross-sections were processed and stained with HE or PAS for morphological analysis.
Immunohistochemical and immunofluorescent staining were performed as follows. Human or rat tissue was fixed in 4% paraformaldehyde, dehydrated, embedded in paraffin wax, and serially sectioned at a thickness of 4 μm. The sections were incubated with primary antibodies overnight at 4 °C. Subsequently, the sections were stained with fluorescent secondary antibodies. The nuclei were stained with DAPI (Invitrogen). The sections were imaged using a fluorescence microscope (Olympus BX53, Tokyo, Japan). A minimum of 5 random images from 3 samples were analyzed per group. Immunohistochemical statistical analysis was conducted with the Fromowitz comprehensive scoring method [30 (link)]. The details of the antibodies are listed in Additional file 1: Supplementary Table 1.
Wang J., Zhang P., Liu M., Huang Z., Yang X., Ding Y., Liu J., Cheng X., Xu S., He M., Zhang F., Wang G., Li R, & Yang X. (2023). Alpha-2-macroglobulin is involved in the occurrence of early-onset pre-eclampsia via its negative impact on uterine spiral artery remodeling and placental angiogenesis. BMC Medicine, 21, 90.
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Publication 2023
Antibodies Cell Nucleus DAPI Eosin Fluorescent Antibody Technique Homo sapiens Microscopy, Fluorescence Paraffin Paraffin Embedding paraform Periodic Acid Tissues
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Immunohistochemical Analysis of AGR2 in HCC
The use of archived formalin-fixed, paraffin-embedded tissue blocks was approved by the Institutional Review Board of National Cheng Kung University Hospital. Tissue slides from HCC patients were evaluated via immunohistochemistry and hematoxylin/eosin staining using a polyclonal antibody against AGR2 (GeneTex, Hsinchu, Taiwan) according to the avidin–biotin complex method, as described previously [31 (link)]. Immunoreactivity for AGR2 was visualized using DAB/nickel substrate (Vector Laboratories, Burlingame, CA).
Tsai H.W., Chen Y.L., Wang C.I., Hsieh C., Lin Y.H., Chu P.M., Wu Y.H., Huang Y.C, & Chen C.Y. (2023). Anterior gradient 2 induces resistance to sorafenib via endoplasmic reticulum stress regulation in hepatocellular carcinoma. Cancer Cell International, 23, 42.
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Publication 2023
Avidin Biotin Cloning Vectors Eosin Ethics Committees, Research Formalin Immunoglobulins Immunohistochemistry Nickel Paraffin Embedding Patients Tissues

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Bx51 microscope by Olympus
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Hematoxylin by Merck Group
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Hematoxylin is a natural dye extracted from the wood of the Logwood tree (Haematoxylum campechianum). It is a commonly used stain in histology and microscopy for the staining of cell nuclei, providing a deep blue-purple color. Hematoxylin is considered a progressive stain, requiring the use of a mordant, such as aluminum salts, to create the desired staining effect.
4
Hematoxylin and eosin by Merck Group
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Hematoxylin and eosin (H&E) is a commonly used staining method in histology and pathology. Hematoxylin stains cell nuclei blue, while eosin stains the cytoplasm and extracellular matrix in various shades of pink and red. This staining technique is used to provide contrast and enable the visualization of cellular and tissue structures under a microscope.
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Optical microscope by Olympus
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More about "Eosin"

Fluorescein, H&E staining, cell morphology, pathology, histology, cytology, Image-Pro Plus, BX51 microscope, Hematoxylin, Optical microscope, Eclipse 80i, Paraformaldehyde, Microtome, Matrigel, Image-Pro Plus software