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Fast Green FCF

Fast Green FCF is a synthetic dye commonly used in food coloring, cosmetics, and pharmaceutical applications.
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Most cited protocols related to «Fast Green FCF»

1
Metagenomic Assembly with metaSPAdes
In addition to single-nucleotide variants and small indels, strain variation is often manifested as highly diverged regions, insertions of mobile elements, rearrangements, large deletions, parallel gene transfer, etc. The green edges in the assembly graph shown in Figure 3 result from an additional copy of a mobile element in a rare strain2 (compared with the abundant strain1), while the blue edge corresponds to a horizontally transferred gene (or a highly diverged genomic region) in a rare strain3 (compared to the abundant strain1). Such edges fragment contigs corresponding to the abundant strain1; for example, the green edges in Figure 3 (bottom right) break the edge c into three shorter edges. We note that the edges in the assembly graph are condensed; that is, they represent nonbranching paths formed by k-mers.
We refer to edges originating from rare strain variants within the assembly graph of a strain mixture as filigree edges. Traditional genome assemblers use a global threshold on read coverage to remove the low-coverage edges (that typically result from sequencing errors) from the assembly graph during the graph simplification step. However, this approach does not work well for metagenomic assemblies, since there is no global threshold that (1) removes edges corresponding to rare strains and (2) preserves edges corresponding to rare species. Similarly to IDBA-UD and MEGAHIT, metaSPAdes analyzes the coverage ratios between adjacent edges in the assembly graph, classifying edges with low-coverage ratios as potential filigree edges.
We denote the coverage of an edge e in the assembly graph as cov(e) and define the coverage cov(v) of a vertex v as the maximum of cov(e) over all edges e incident to v. Given an edge e incident to a vertex v and a threshold ratio (the default value is 10), a vertex v predominates an edge e if its coverage is significantly higher than the coverage of the edge e; that is, if ratio · cov(e) < cov(v). An edge (v,w) is weak if it is predominated by either v or w. Note that filigree edges are often classified as weak since their coverage is much lower than the coverage of adjacent edges resulting from abundant strains.
metaSPAdes disconnects all weak edges from their predominating vertices in the assembly graph. Disconnection of a weak edge (v,w) in the assembly graph from its starting vertex v (ending vertex w) is simply a removal of its first (last) k-mer rather than removal of the entire condensed edge. We emphasize that, in contrast to IDBA-UD and MEGAHIT, we disconnect rather than remove weak edges in the assembly graph since our goal is to preserve the information about rare strains whenever possible, that is, when it does not lead to a deterioration of the consensus backbone.
Nurk S., Meleshko D., Korobeynikov A, & Pevzner P.A. (2017). metaSPAdes: a new versatile metagenomic assembler. Genome Research, 27(5), 824-834.
Publication 2017
Debility DNA Insertion Elements Fast Green FCF Gene Deletion Gene Rearrangement Genes Gene Transfer, Horizontal Genome INDEL Mutation Metagenome Nucleotides Strains Vertebral Column
2
Consensus on Sjögren's Syndrome Criteria
In February 2004, the panel of experts gathered for a face-to-face meeting moderated by a statistician (SCS) and an epidemiologist (CHS). The goal of this meeting was to obtain consensus (at least 80%) on the target population to whom the classification criteria would apply, and the initial list of variables or criteria items that would be collected as part of SICCA. The meeting began with presentation of a comprehensive literature review by one of the senior investigators (TED) of the 11 previous classification and diagnostic criteria for SS that had been published in the past 40 years, none of which had been endorsed by the ACR or EULAR.
There was consensus among the panel that the criteria should apply to the population of patients who may be referred to a specialist because of signs and/or symptoms possibly suggesting SS. Recruitment strategies and eligibility criteria are described below. The rationale for selecting this target population is that a given patient would not be evaluated for SS unless she/he had signs or symptoms suggesting this diagnosis. There was also consensus that if asked to select cases and controls for validation of new classification criteria, panel members would use objective tests (e.g., specific serum measures of autoimmunity, ocular staining reflecting lacrimal hypofunction, and LSG biopsy reflecting FLS) that would likely be part of the new classification criteria, leading to circularity. Therefore, it was agreed that no diagnostic labels would be used for enrollment, and that all participants would undergo the same set of standardized objective tests, and questionnaires capturing various signs and symptoms.
The panel agreed upon examinations and tests used to assess ocular and oral signs and symptoms, tear and salivary function, LSG biopsy results and various serum measures of autoimmunity. The list created was based both on published results and on the clinical experience of panel members. There was discussion among the rheumatologists regarding which extra-glandular manifestations possibly associated with SS should be captured, and a consensus was achieved regarding a list of signs/symptoms that would be measured through a targeted rheumatologic examination, review of systems, careful medical history and serologic laboratory measures. Similarly, the oral medicine specialists agreed on a list of tests measuring salivary function (both stimulated parotid and UWS flow rates), and salivary gland expression of autoimmunity through biopsy of LSG, examining them for the presence of FLS, and measuring FS accordingly as described in detail elsewhere (15 (link)). The ophthalmologists agreed on tests evaluating participants for the presence of keratoconjunctivitis sicca (KCS). There was consensus that, while rose Bengal had been widely used for grading conjunctival and corneal damage in patients with KCS, it is inherently toxic to epithelial cells and very painful for patients. Therefore, fluorescein was selected to grade the cornea and lissamine green the bulbar conjunctiva. Effectiveness for grading KCS is established for both (16 (link)). They agreed on a standardized quantitative grading system that would be easily reproducible and could be used in clinical practice in the future (17 (link)). Ocular staining score (OSS) is the sum of a 0–6 score for fluorescein staining of the cornea and a 0–3 score for lissamine green staining of both nasal and temporal bulbar conjunctivae, yielding a total score ranging from 0 to 12. Alternative established tests for dryness used in prior criteria, such as tear break-up time (TBUT) and unanesthetized Schirmer test, were also included.
The final list of criteria items that was agreed upon by the end of the first meeting included nearly all those previously reported in the relevant literature. It has been described previously (12 (link)) and is available at http://sicca.ucsf.edu.
Shiboski S., Shiboski C., Criswell L., Baer A., Challacombe S., Lanfranchi H., Schiødt M., Umehara H., Vivino F., Zhao Y., Dong Y., Greenspan D., Heidenreich A., Helin P., Kirkham B., Kitagawa K., Larkin G., Li M., Lietman T., Lindegaard J., McNamara N., Sack K., Shirlaw P., Sugai S., Vollenweider C., Whitcher J., Wu A., Zhang S., Zhang W., Greenspan J, & Daniels T. (2012). American College of Rheumatology Classification Criteria for Sjögren’s Syndrome: A Data-Driven, Expert Consensus Approach in the SICCA Cohort. Arthritis care & research, 64(4), 475-487.
Publication 2012
Autoimmunity Biopsy Conjunctiva Conjunctiva, Bulbar Cornea Cornea Injuries Diagnosis Eligibility Determination Epidemiologists Epithelial Cells Eye Face Fast Green FCF Fluorescein Keratoconjunctivitis Sicca Nose Ophthalmologists Pain Parotid Gland Patients Physical Examination Rheumatologist Rose Bengal Salivary Glands Serum Signs and Symptoms Specialists Target Population Tears
3
Targeted Viral Labeling of Nodose/Jugular Complex

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Publication 2015
Adult Anesthesia Animals Cloning Vectors Dissection Fast Green Fast Green FCF Ganglia Genome Mice, House Neck Operative Surgical Procedures tdTomato Tissue Harvesting Virus
4
Liver Injury and Fibrosis Evaluation
At times indicated in the figure legends, mice were anesthetized with pentobarbital (80 mg/kg, i.p.), and blood was collected from inferior vena cava. Livers were harvested and slides were prepared as described elsewhere (Zhong, et al., 2003 (link)). In sections stained with hematoxylin and eosin (H+E), ten random fields per slide were captured in a blinded manner using a Universal Imaging Image-1/AT image acquisition and analysis system (West Chester, PA) with an Axioskop 50 microscope (Carl Zeiss, Inc., Thornwood, NY) and a 10× objective lens. Necrotic areas were quantified by image analysis using IP Lab 3.7v software (BD Biosciences, Rockville, MD) by dividing necrotic areas by total cellular area of the images. Some slides were stained with 0.1% Sirius red (Polysciences Inc., Warrington, PA) and fast green FCF (Sigma Chemical, St. Louis, MO) to evaluate liver fibrosis, and Sirius red-positive area was quantified as described (Zhong, et al., 2003 (link)). In other experiments, liver tissue was frozen and stored at -80°C for later mRNA analysis. Serum alanine transaminase (ALT) was measured using a kit from Pointe Scientific (Uncoln Park, MI).
Rehman H., Ramshesh V.K., Theruvath T.P., Kim I., Currin R.T., Giri S., Lemasters J.J, & Zhong Z. (2008). NIM811, a Mitochondrial Permeability Transition Inhibitor, Attenuates Cholestatic Liver Injury But Not Fibrosis in Mice. The Journal of pharmacology and experimental therapeutics, 327(3), 699-706.
Publication 2008
Alanine Transaminase BLOOD Cells Eosin Fast Green FCF Fibrosis, Liver Freezing Lens, Crystalline Liver Mice, House Microscopy Necrosis Pentobarbital RNA, Messenger Serum Tissues Vena Cavas, Inferior
5
Quantitative Fluorescence Imaging of Tumor Vasculature
Fluorescence analyses were performed using an Olympus MVX10 microscope with a 2X objective (NA, 0.5) and an optical zoom of 0.63-6.3x. Excitation and emission filters were 470 ± 40 nm and 525 ± 50 nm for eGFP, 560 ± 55 nm and 645 ± 75 nm for Texas Red dextran, and 740 ± 35 nm and 780 longpass filter for near-infrared indocyanine green. Exposure time varied from 300-500 ms for initial scans of whole tissue sections to 15 ms for quantitative analysis of tumor regions. For Texas Red dextran, total fluorescence intensity in a region of interest was converted to sum voxel intensity/g tissue. Volume was calculated as area (cm2) × 0.002 cm thickness corrected for density 1.04 g/cm3. To convert fluorescence intensity to concentration, standard curves were generated (Supplementary Fig. 3), similar to autoradiography (46 (link)). Brain (500 mg) was excised and homogenized to uniformity with 100 μL of saline containing different concentrations of Texas Red dextran. The final mixture was flash frozen in isopentane and sliced into 20 μm sections. Similarly, blood samples were spiked with concentrations of dye, 1 μL samples were placed on glass slides and dried, and then total fluorescence intensity for the blood drop was measured. Texas Red dextran fluorescence intensity did not differ between standards prepared from brain or tumor, or from solutions of differing pH (6.0 −7.6) or Na+/Ca2+ concentration (data not shown). Texas Red dextran sum intensity was stable within ± 5% with repeat fluorescent exposures (15 ms – 1500 ms). Fluorescent image analysis was performed using Slidebook 5.0 program (Olympus). Vascular density and surface area were calculated using binary masks where vessels were defined by indocyanine green fluorescence ≥3 fold above background.
Lockman P.R., Mittapalli R.K., Taskar K.S., Rudraraju V., Gril B., Bohn K.A., Adkins C.E., Roberts A., Thorsheim H.R., Gaasch J.A., Huang S., Palmieri D., Steeg P.S, & Smith Q.R. (2010). Heterogeneous Blood-Tumor Barrier Permeability Determines Drug Efficacy in Experimental Brain Metastases of Breast Cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 16(23), 5664-5678.
Publication 2010
Autoradiography BLOOD Blood Vessel Brain Dextran dextran - saline drug combination Dextran 75 Fast Green FCF Fluorescence Freezing Indocyanine Green isopentane Microscopy Neoplasms Radionuclide Imaging Tandem Mass Spectrometry Tissues

Most recents protocols related to «Fast Green FCF»

1
Green Tea Infused Silk Gel
Not available on PMC !

Example 10

Steps:
Green Tea PrepHeat 250 mL water to boil
Steep tea bag 2-3 minutes
with occasional stir
remove tea bag and let cool
Gel SolutionUse TFF-10-0047 (3.71% silk)
Prepdilute to 3% silk with water
dilute to 2% with green tea
add L-ascorbic acid
GelGelation occurred like standard
gel at room temperature
Green/yellow color
Green Tea scent
Solution Spec:2% silk solution
65 mL (35 ml of 3.71% silk, 8.3
mL water, 21.66 mL green tea)
0.43 gL-ascorbic acid

FIG. 92 is a table summarizing an embodiment of a caffeine gel of the present disclosure. A silk gel with 2% silk and 100 mg L-ascorbic acid/15 mL solution was created with the addition of 50 mg caffeine/15 mL solution. The gel has the exact appearance of standard L-ascorbic acid gels.

US11857663B2. Stable silk protein fragment compositions (2024-01-02). Evolved By Nature, Inc. [US]. Inventors: Gregory H. Altman [US], Rebecca L. Lacouture [US], Rachel Lee Dow [US], Rachel M. Lind [US], Dylan S. Haas [US].
Patent 2024
Ascorbic Acid Caffeine Fast Green FCF Furuncles Gels Green Tea Pheromone Silk STEEP1 protein, human Technique, Dilution
2
Green Tea and Silk Nanocomposite Gel
Not available on PMC !

Example 10

Steps:

    • Green Tea Prep Heat 250 mL water to boil
      • Steep tea bag 2-3 minutes with occasional
      • stir
      • remove tea bag and let cool
    • Gel Solution
    • Prep Use TFF-10-0047 (3.71% silk)
      • dilute to 3% silk with water
      • dilute to 2% with green tea
      • add L-ascorbic acid
      • Gelation occurred like standard gel at room
    • Gel temperature
      • Green/yellow color
      • Green Tea scent
    • Solution Spec: 2% silk solution
      • 65 mL (35 ml of 3.71% silk, 8.3 mL water,
      • 21.66 mL green tea)
      • 0.43 g L-ascorbic acid

FIG. 92 is a table summarizing an embodiment of a caffeine gel of the present disclosure. A silk gel with 2% silk and 100 mg L-ascorbic acid/15 mL solution was created with the addition of 50 mg caffeine/15 mL solution. The gel has the exact appearance of standard L-ascorbic acid gels.

US11857664B2. Stable silk protein fragment compositions (2024-01-02). Evolved By Nature, Inc. [US]. Inventors: Gregory H. Altman [US], Rebecca L. Lacouture [US], Rachel Lee Dow [US], Rachel M. Lind [US], Dylan S. Haas [US].
Patent 2024
Ascorbic Acid Caffeine Fast Green FCF Furuncles Gels Green Tea Pheromone Silk STEEP1 protein, human Technique, Dilution
3
Quantifying Meristematic Characteristics in Lateral Roots
The lateral roots were fixed in formalin–acetic acid–methanol (FAA) according to the method of Livingston et al.62 (link). Blocks were sectioned with a Leica RM2016 rotary microtome at 5 μm. Fast green FCF and safranin O were used to stain the sections. The lignified or corkified cell wall and vessel element will be dyed red and other tissues will be dyed green. Images were captured with a Nikon Eclipse E100. Subsequently, the length of the meristematic zone and the number of meristematic cells were analyzed using ImageJ 1.53.
Wang S., Feng Y., Lou Y., Niu J., Yin C., Zhao J., Du W, & Yue A. (2023). 3-Hydroxy-3-methylglutaryl coenzyme A reductase genes from Glycine max regulate plant growth and isoprenoid biosynthesis. Scientific Reports, 13, 3902.
Publication 2023
Acetic Acid Blood Vessel Cell Wall Fast Green FCF Formalin Meristem Methanol Microtomy Plant Roots safranine T Stains Tissues
4
Detailed Paraffin Sectioning Protocol
For the paraffin sections, fully unfolded leaves and stems at the same position were detached from the transgenic and WT plants grown in the greenhouse, fixed with FAA [5% (v/v) glacial acetic acid, 5% (v/v) formalin, and 50% (v/v) ethanol] solution, and evacuated for 30 min with a vacuum pump. The fixed materials were then dehydrated with gradient ethanol (50%, 70%, 80%, 90%, 95%, and 100%, v/v) and gradient xylene (25%, 50%, 75%, and 100% xylene in ethanol, v/v). Subsequently, the samples were soaked in paraffin at 60°C for three days (the paraffin solution was replaced three times during this period), after which they were embedded in a paraffin block. Approximately 8-μm sections were obtained using a microtome (LEICA RM2265, Leica, Germany), dewaxed in xylene, and then stained with safranin and Fast Green FCF. Finally, the images of these sections were observed using an Echo Revolve light microscope (ECHO, San Diego, CA, USA).
Sun S., Li X., Nie N., Chen Y., Gao S., Zhang H., He S., Liu Q, & Zhai H. (2023). Sweet potato NAC transcription factor NAC43 negatively regulates plant growth by causing leaf curling and reducing photosynthetic efficiency. Frontiers in Plant Science, 14, 1095977.
Publication 2023
Acetic Acid Animals, Transgenic ECHO protocol Ethanol Fast Green FCF Formalin Light Microscopy Microtomy Paraffin Paraffin Embedding Plants safranine T Stem, Plant Vacuum Xylene
5
Caspase 3/7 Analysis of Apoptotic Cells in Scaffolds
The distribution of apoptotic cells within scaffolds for all conditions under study was evaluated via Caspase 3/7 analysis. More specifically, scaffolds were snap frozen at time of points 1 and 7 days post-treatment in liquid nitrogen for 20 min and preserved thereafter at –80°C. The scaffolds were sectioned and stained with Cell Event Caspase 3/7 green detection reagent (Fisher Scientific, UK) and DAPI (Fisher Scientific, UK) for 1 h at 37°C. Thereafter, the samples were washed with PBS and visualised using a Nikon Ti-Eclipse inverted confocal microscope (Nikon Instruments, Europe).
Wishart G., Gupta P., Nisbet A., Velliou E, & Schettino G. (2023). Enhanced effect of X-rays in the presence of a static magnetic field within a 3D pancreatic cancer model. The British Journal of Radiology, 96(1143), 20220832.
Publication 2023
Apoptosis Caspase Caspase-7 Caspase 3 Cells DAPI Fast Green FCF Freezing Microscopy, Confocal Nitrogen

Top products related to «Fast Green FCF»

1
Fast green fcf by Merck Group
Sourced in United States, Germany, United Kingdom, Ireland
Fast Green FCF is a synthetic dye commonly used as a food coloring agent and in laboratory applications. It is a dark green, crystalline powder that is soluble in water and alcohol. Fast Green FCF is primarily used for staining and visualization purposes in various scientific and industrial processes.
2
Cellevent caspase 3 7 green detection reagent by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, Japan, Sweden, Italy, Canada
The CellEvent Caspase-3/7 Green Detection Reagent is a fluorogenic substrate for the detection of caspase-3 and caspase-7 activity in live cells. It consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. Upon cleavage by active caspase-3 and caspase-7, the dye is released and can bind to DNA, resulting in a bright green fluorescent signal.
3
Cellevent caspase 3 7 green flow cytometry assay kit by Thermo Fisher Scientific
Sourced in United States, Sweden
The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit is a laboratory instrument designed to detect and quantify the activation of caspase-3 and caspase-7 enzymes in cells. It utilizes a fluorogenic substrate that emits a green fluorescent signal upon cleavage by these caspases, allowing for the measurement of apoptosis in cell samples using flow cytometry.
4
Safranin o by Merck Group
Sourced in United States, Germany, Japan, Canada, United Kingdom, Italy, China, Sao Tome and Principe, Switzerland
Safranin O is a cationic red dye used in various laboratory applications. It is a staining agent commonly employed in microscopy and microbiology for the identification and visualization of cellular structures.
5
Direct red 80 by Merck Group
Sourced in United States, Germany, Japan, United Kingdom, China, France, Italy, Netherlands, Canada
Direct Red 80 is a synthetic dye commonly used in laboratory settings. It is a water-soluble, red-colored azo dye that is utilized in various applications, such as staining and coloring procedures. The core function of Direct Red 80 is to provide a consistent and reliable coloring agent for specific laboratory techniques and processes. Further details on its intended use are not available.
6
Fast green fcf by Thermo Fisher Scientific
Sourced in United States
Fast Green FCF is a synthetic dye used in various laboratory applications. It is a green-colored compound that can be used as a staining agent or a colorant. The core function of Fast Green FCF is to provide a visual marker or contrast in various types of laboratory experiments and procedures.
7
Quantstudio 3 real time pcr system by Thermo Fisher Scientific
Sourced in United States, China, Japan, Germany, Canada, Switzerland, Italy, United Kingdom, Spain, Belgium, Lithuania, Singapore, France, Australia
The QuantStudio 3 Real-Time PCR System is a thermal cycler designed for real-time quantitative polymerase chain reaction (qPCR) analysis. It is capable of performing precise and sensitive DNA amplification and detection in a 96-well format.
8
Modified safranine o fast green fcf cartilage stain kit by Solarbio
Sourced in China
The Modified Safranine O-Fast Green FCF Cartilage Stain Kit is a laboratory product designed for staining and visualizing cartilage tissues. It contains reagents required for the staining process.
9
Incucyte zoom by Sartorius
Sourced in United States, United Kingdom, Germany, Japan
The IncuCyte ZOOM is a live-cell analysis system that enables real-time monitoring and quantification of cellular processes. It provides automated, non-invasive, and time-lapse imaging capabilities to observe and measure cellular responses within a controlled environment.
10
Incucyte caspase 3 7 green apoptosis assay reagent by Sartorius
Sourced in United Kingdom, Germany
The IncuCyte Caspase-3/7 Green Apoptosis Assay Reagent is a fluorescent probe designed to detect caspase-3 and caspase-7 activity in living cells. It provides a real-time, quantitative measurement of apoptosis in cell culture.

More about "Fast Green FCF"

Fast Green FCF is a synthetic dye used extensively in various industries, including food coloring, cosmetics, and pharmaceuticals.
This vibrant green dye has a wide range of applications and is often used as a coloring agent or stain in various research and laboratory settings.
Beyond Fast Green FCF, other related dyes and compounds have specific uses in the scientific community.
For instance, CellEvent Caspase-3/7 Green Detection Reagent is a fluorescent probe used to detect caspase-3 and caspase-7 activity, which are key indicators of apoptosis, or programmed cell death.
The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit is another tool that allows researchers to quantify apoptosis using flow cytometry.
Safranin O and Direct Red 80 are also commonly used stains, often employed in the histological analysis of tissues, particularly in the evaluation of cartilage and bone.
The Modified Safranine O-Fast Green FCF Cartilage Stain Kit, for example, combines these dyes to enable the visualization and assessment of cartilage samples.
Researchers may also utilize specialized equipment, such as the QuantStudio 3 Real-Time PCR System, to conduct precise, quantitative analyses, and the IncuCyte ZOOM and IncuCyte Caspase-3/7 Green Apoptosis Assay Reagent to monitor cellular processes in real-time.
PubCompare.ai, an AI-powered platform, can assist researchers in optimizing their studies on Fast Green FCF and related compounds by providing access to a wealth of published protocols, preprints, and patents.
This tool helps ensure reproducibility and accuracy, saving time and improving experimental outcomes.