2-Iodobenzonitrile, ethyl-1H-indole-carboxylate and 5-bromo-ethyl-1H-indole-carboxylate were purchased from ABCR. Borane solution (1M in THF), absolute DMF, dimethylaminopyridine, di-tert-butyl-dicarbonate, absolute acetonitrile, palladium(II) acetate, sodium bicarbonate, basic aluminuim oxide, 2-acetylpyridine and 2-formylpyridine were bought from Fisher/Acros Organics. Ethoxy-methylchloride was obtained form TCI. Sodium hydride, phosphorus(V) sulfide, celite, hydrazine monohydrate and methyl iodide were purchased from Sigma Aldrich, while lithium hydroxide monohydrate and triphenylphosphine were from Alfa Aesar. 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide-hydrochloride was purchased from IRIS biotech. Silver(I) carbonate was purchased from Merck. 2-Iodobenzylamine was prepared by a known method.26 (link) The unsubstituted indolo[2,3-d]benzazepinone (A ) was prepared by following published protocols.18 (link)–20 The 11-bromo-substituted B was prepared using reported precedures,18 (link)–20 with some modifications, a detailed description of the synthesis of B is given in the Supplementary Information file.
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Boranes
Boranes
Boranes are a class of chemical compounds composed of boron and hydrogen atoms.
These versatile molecules have a wide range of applications in chemistry, materials science, and energy research.
Borane compounds exhibit unique structural and reactivity properties, making them valuable tools for synthetic transformations, catalysis, and the development of novel materials.
PubCompare.ai leverages advanced AI algorithms to help researchers identify the most accurate and reproducible protocols for working with boranes, enabling reliable, high-quality results in their scientific endeavors.
Explore the power of AI-driven protocol comparison and discover the future of borane research today.
These versatile molecules have a wide range of applications in chemistry, materials science, and energy research.
Borane compounds exhibit unique structural and reactivity properties, making them valuable tools for synthetic transformations, catalysis, and the development of novel materials.
PubCompare.ai leverages advanced AI algorithms to help researchers identify the most accurate and reproducible protocols for working with boranes, enabling reliable, high-quality results in their scientific endeavors.
Explore the power of AI-driven protocol comparison and discover the future of borane research today.
Most cited protocols related to «Boranes»
2-acetylpyridine
Acetate
acetonitrile
Anabolism
Bicarbonate, Sodium
bis(tert-butoxycarbonyl)oxide
Boranes
Carbodiimides
Carbonates
Celite
hydrazine hydrate
indole
Iris Plant
lithium hydroxide monohydrate
Methyl Chloride
methyl iodide
Oxides
Palladium
Phosphorus
Silver
sodium hydride
Sulfides
triphenylphosphine
Glycan release and labeling of Korčula and Vis IgG samples was performed essentially as described by Royle and coworkers (46 (link)). Briefly, IgG was immobilized in a block of sodium dodecyl sulfate–polyacrylamide gel and N-glycans were released by digestion with PNGase F (ProZyme, Hayward, CA). Each step was done in a 96-well microtiter plate to achieve the best throughput of sample preparation. After deglycosylation, N-glycans were labeled with 2-AB fluorescent dye. Excess of label was removed by solid-phase extraction using Whatman 3MM chromatography paper. Finally, glycans were eluted with water and stored at −20°C until usage.
Orkney and TwinsUK IgG samples were first denatured with addition of 30 μL 1.33% sodium dodecyl sulfate (w/v) (Invitrogen, Carlsbad, CA) and by incubation at 65°C for 10min. Subsequently, 10 μL of 4% Igepal-CA630 (Sigma–Aldrich, St. Louis, MO) and 1.25 mU of PNGase F (ProZyme) in 10 μL 5× phosphate-buffered saline were added to the samples. The samples were incubated overnight at 37°C for N-glycan release. The released N-glycans were labeled with 2-AB. The labeling mixture was freshly prepared by dissolving 2-AB (Sigma–Aldrich) in dimethyl sulfoxide (Sigma–Aldrich) and glacial acetic acid (Merck) mixture (85:15, v/v) to a final concentration of 48mg/mL. A volume of 25 μL of labeling mixture was added to each N-glycan sample in the 96-well plate. Also, 25 μL of freshly prepared reducing agent solution (106.96mg/mL 2-picoline borane [Sigma–Aldrich] in dimethyl sulfoxide) was added and the plate was sealed using adhesive tape. Mixing was achieved by shaking for 10min, followed by 2-hour incubation at 65°C. Samples (in a volume of 100 μL) were brought to 80% acetonitrile (ACN) (v/v) by adding 400 μL of ACN (J.T. Baker, Phillipsburg, NJ). Free label and reducing agent were removed from the samples using hydrophilic interaction chromatography–solid-phase extraction. An amount of 200 μL of 0.1g/mL suspension of microcrystalline cellulose (Merck) in water was applied to each well of a 0.45 μm GHP filter plate (Pall Corporation, Ann Arbor, MI). Solvent was removed by application of vacuum using a vacuum manifold (Millipore Corporation, Billerica, MA). All wells were prewashed using 5×200 μL water, followed by equilibration using 3×200 μL acetonitrile/water (80:20, v/v). The samples were loaded to the wells. The wells were subsequently washed seven times using 200 μL acetonitrile/water (80:20, v/v). Glycans were eluted two times with 100 μL of water and combined eluates were stored at −20°C until usage.
Orkney and TwinsUK IgG samples were first denatured with addition of 30 μL 1.33% sodium dodecyl sulfate (w/v) (Invitrogen, Carlsbad, CA) and by incubation at 65°C for 10min. Subsequently, 10 μL of 4% Igepal-CA630 (Sigma–Aldrich, St. Louis, MO) and 1.25 mU of PNGase F (ProZyme) in 10 μL 5× phosphate-buffered saline were added to the samples. The samples were incubated overnight at 37°C for N-glycan release. The released N-glycans were labeled with 2-AB. The labeling mixture was freshly prepared by dissolving 2-AB (Sigma–Aldrich) in dimethyl sulfoxide (Sigma–Aldrich) and glacial acetic acid (Merck) mixture (85:15, v/v) to a final concentration of 48mg/mL. A volume of 25 μL of labeling mixture was added to each N-glycan sample in the 96-well plate. Also, 25 μL of freshly prepared reducing agent solution (106.96mg/mL 2-picoline borane [Sigma–Aldrich] in dimethyl sulfoxide) was added and the plate was sealed using adhesive tape. Mixing was achieved by shaking for 10min, followed by 2-hour incubation at 65°C. Samples (in a volume of 100 μL) were brought to 80% acetonitrile (ACN) (v/v) by adding 400 μL of ACN (J.T. Baker, Phillipsburg, NJ). Free label and reducing agent were removed from the samples using hydrophilic interaction chromatography–solid-phase extraction. An amount of 200 μL of 0.1g/mL suspension of microcrystalline cellulose (Merck) in water was applied to each well of a 0.45 μm GHP filter plate (Pall Corporation, Ann Arbor, MI). Solvent was removed by application of vacuum using a vacuum manifold (Millipore Corporation, Billerica, MA). All wells were prewashed using 5×200 μL water, followed by equilibration using 3×200 μL acetonitrile/water (80:20, v/v). The samples were loaded to the wells. The wells were subsequently washed seven times using 200 μL acetonitrile/water (80:20, v/v). Glycans were eluted two times with 100 μL of water and combined eluates were stored at −20°C until usage.
Acetic Acid
acetonitrile
Boranes
Chromatography
Digestion
Fluorescent Dyes
Glycopeptidase F
Hydrophilic Interactions
microcrystalline cellulose
Phosphates
Picoline
polyacrylamide gels
Polysaccharides
Reducing Agents
Saline Solution
Solid Phase Extraction
Solvents
Sulfate, Sodium Dodecyl
Sulfoxide, Dimethyl
Vacuum
1H NMR
2-picoline
Acetic Acid
acetonitrile
Bicarbonate, Sodium
Boranes
brine
Chromatography
ethyl acetate
Methanol
Solvents
Spectinomycin
acetonitrile
Ammonium
Bath
Boranes
Buffers
Centrifugation
Digestion
Ethanol
formic acid
Glycopeptidase F
Glycoproteins
Methanol
methyl iodide
Oligosaccharides
Polysaccharides
Proteins
Salts
Serum
Sodium Hydroxide
Sulfoxide, Dimethyl
Tandem Mass Spectrometry
Vacuum
acetamide
acetic anhydride
acetonitrile
Acids
Amides
Amines
Amino Acids
Anabolism
Boranes
Carbamates
chiralpak AS
Crystallization
derivatives
Diamines
Esters
Formaldehyde
High-Performance Liquid Chromatographies
Isomerism
nitroethane
Retention (Psychology)
Sodium
Stereoisomers
tartaric acid
Thiophene
triethylamine
Urea
Most recents protocols related to «Boranes»
All chemicals including cobalt nitrate hexahydrate (Co(NO3)2·6H2O, AR), copper nitrate trihydrate (Cu(NO3)2·3H2O, AR), sodium hypophosphite monohydrate (NaH2PO2·H2O, AR), potassium hydroxide (KOH, AR), hydrochloric acid (HCl, 37%), and ammonia borane (NH3BH3, 97%) were purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd, China. Deionized water was used throughout this experiment.
Ammonia
Boranes
cobaltous nitrate hexahydrate
Copper
Hydrochloric acid
Nitrates
potassium hydroxide
sodium hypophosphite monohydrate
For performing the photocatalytic
H2 production reaction from ammonia borane (NH3BH3), powder samples of KTLO, HTO, exf-HTO, and Sn/exf-HTO
were dispersed in 4.5 mL of distilled water in a Pyrex test tube purged
with N2 for 30 min. Before sealing with a rubber septum
properly, 0.5 mL of 40 mM NH3BH3 was added.
For the H2 evolution reaction from water, powder samples
(5 mg) were dispersed separately in 5 mL of distilled water (20%V/V
methanol) in a Pyrex test tube which was sealed properly and purged
with N2 for 30 min. The test tube was illuminated via the
solar simulator while stirring constantly. The amount of H2 produced was measured with the gas chromatograph, directly taking
injections from the headspace of the Pyrex tube using a gastight syringe.
H2 production reaction from ammonia borane (NH3BH3), powder samples of KTLO, HTO, exf-HTO, and Sn/exf-HTO
were dispersed in 4.5 mL of distilled water in a Pyrex test tube purged
with N2 for 30 min. Before sealing with a rubber septum
properly, 0.5 mL of 40 mM NH3BH3 was added.
For the H2 evolution reaction from water, powder samples
(5 mg) were dispersed separately in 5 mL of distilled water (20%V/V
methanol) in a Pyrex test tube which was sealed properly and purged
with N2 for 30 min. The test tube was illuminated via the
solar simulator while stirring constantly. The amount of H2 produced was measured with the gas chromatograph, directly taking
injections from the headspace of the Pyrex tube using a gastight syringe.
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Ammonia
Biological Evolution
Boranes
Gas Chromatography
Powder
Rubber
Syringes
A total of 40 mg of magnetic NHG’s (50 nm) matrix and 79 mg boric acid (1.27 mmol) were taken in a reactor. A total of 150 µL of trimethyl borate (1.27 mmol) was added followed by the addition of 4.25 mL 1 M borane-THF complex (4.25 mmol). After the cessation of hydrogen evolution, the reactor was capped tightly and kept in an oil bath at 65 °C for 96 h. The matrix was then centrifuged and washed with MeOH (20 mL × 5), DMF (20 mL × 5), and water (20 mL × 5) using magnetic susceptibility for separation. The resulting matrix was kept in 10 mL H2O.
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Bath
Biological Evolution
Boranes
boric acid
Hydrogen
Susceptibility, Disease
trimethyl borate
The centrifuged polymeric NHG’s (160 mg) and 319 mg boric acid (5.16 mmol) was poured into a glass pressure tube. A total of 607 µL of trimethyl borate (5.16 mmol) was added followed by the addition of 17 mL 1 M borane-THF complex (17 mmol). After the cessation of hydrogen evolution, the tubes were sealed and kept in an oil bath at 65 °C for 72 h. The NHG’s were then centrifuged and washed with MeOH (10 mL × 2). The obtained precipitate was mixed with methanol (8 mL) and piperidine (2 mL), transferred to a reactor, and heated to 65°C for 20 h under pressure to destroy the borane complexes. Following the decantation of the piperidine-borane solution, water was added, and the suspension was centrifuged and washed with distilled water (10 mL × 3). The reduced NHGs were dialyzed against 25 L DDW for 2 days. The water was changed twice per day.
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Bath
Biological Evolution
Boranes
boric acid
Hydrogen
Methanol
piperidine
Polymers
Pressure
trimethyl borate
To obtain –OH functional end groups, the poly(butadiene) of exclusively (~100%) -1,2 microstructure is submitted to post-polymerization chemical modification reactions, namely hydroboration and oxidation. In this process, each vinyl bond (-CH=CH2 per monomeric unit) is converted to -CH2-CH2-OH, leading to the desired -OH group in all monomeric units of the PB blocks. To perform the hydroboration reaction 0.15 g (3.3 mmol, 6.6 mL) of 9-BBN were introduced into 1 g of PS-b-PB1,2-b-PDMS (sample 1, corresponding to 2.7 mmoles of the PB1,2 segment) which was dissolved in tetrahydrofuran (0.2 w/v%) under nitrogen atmosphere at −15 °C. The solution was left to warm up to ambient conditions and was stirred for 24 h to ensure the completion of the hydroboration process. Notably, the 9-BBN is used in ~20% excess compared to the mol of the PB1,2 block. Following this, the solution was placed at −25 °C, and 1 mL of properly degassed methanol was added to deactivate all the excess of the borane reagent. After 30 min, NaOH (3.1 mmoles 6 N, 10% excess compared to the borane moles) was introduced to prevent the development of crosslinked networks due to borane by-products. Following, the oxidizing agent H2O2 (6.2 mmoles 30% w/v solution, 50% excess compared to the NaOH moles) was added, and the solution was stirred at −25 °C for 2 h before being placed at 55 °C for 1 h, where the separation of the desired organic and aqueous phases occurred. The organic phase was poured into a 0.25 M NaOH solution, and subsequently, the polymer was dissolved in THF/MeOH and washed with 0.25 M NaOH twice using a Buhner funnel [28 (link),29 (link)]. Finally, the polymer was thoroughly washed with copious amounts of distilled water and placed in a vacuum oven to remove any volatile compounds. Coherent procedures were employed in all terpolymers. The chemical modification reactions for sample PB1,2-b-PS-b-PDMS or sample 3 are provided in Scheme 1 c. Similar reactions are followed for the other sequence of PS-b-PB1,2-b-PDMS samples.
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1,3-butadiene
Atmosphere
Boranes
Methanol
Moles
Nitrogen-15
Oxidants
Peroxide, Hydrogen
Poly A
Polymerization
Polymers
Polyvinyl Chloride
tetrahydrofuran
Vacuum
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
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Borane-ammonia complex is a stable, white, crystalline compound. It is commonly used as a reducing agent in organic synthesis. The compound consists of a borane (BH3) molecule coordinated to an ammonia (NH3) molecule, forming a stable complex.
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Formic acid is a colorless, pungent-smelling liquid chemical compound. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid is widely used in various industrial and laboratory applications.
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Iodomethane is a colorless, volatile organic compound used as a laboratory reagent. It has the chemical formula CH3I and is typically employed as a methylating agent in various chemical reactions and synthesis procedures.
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PNGase F is an enzyme that cleaves the bond between the asparagine residue and the N-acetylglucosamine residue in N-linked glycoproteins. It is commonly used in the analysis and characterization of glycoproteins.
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Sodium hydroxide beads are a chemical compound used in various laboratory and industrial applications. They are composed of small, solid pellets or beads of sodium hydroxide, a strong base. Sodium hydroxide beads are known for their ability to absorb and neutralize acids, making them a valuable tool for pH adjustment and control in various processes.
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Acetic acid is a colorless, vinegar-like liquid chemical compound. It is a commonly used laboratory reagent with the molecular formula CH3COOH. Acetic acid serves as a solvent, a pH adjuster, and a reactant in various chemical processes.
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Methanol is a colorless, volatile, and flammable liquid chemical compound. It is commonly used as a solvent, fuel, and feedstock in various industrial processes.
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Acetonitrile is a highly polar, aprotic organic solvent commonly used in analytical and synthetic chemistry applications. It has a low boiling point and is miscible with water and many organic solvents. Acetonitrile is a versatile solvent that can be utilized in various laboratory procedures, such as HPLC, GC, and extraction processes.
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Ammonia borane is a colorless, crystalline solid chemical compound with the formula BH3NH3. It is a hydrogen-rich material that can be used as a potential hydrogen storage medium.
More about "Boranes"
Boranes are a versatile class of chemical compounds composed of boron and hydrogen atoms.
These unique molecules have a wide range of applications in chemistry, materials science, and energy research.
BH3 compounds, also known as borane or borohydride, exhibit distinctive structural and reactivity properties, making them valuable tools for synthetic transformations, catalysis, and the development of novel materials.
Boranes can be used in a variety of applications, including as reducing agents, in the synthesis of other boron-containing compounds, and in the production of advanced materials.
Related terms include diborane (B2H6), trimethylborane (B(CH3)3), and ammonia borane (NH3BH3), which is a promising hydrogen storage material.
PubCompare.ai leverages advanced AI algorithms to help researchers identify the most accurate and reproducible protocols for working with boranes, enabling reliable, high-quality results in their scientific endeavors.
By exploring the power of AI-driven protocol comparison, researchers can discover the future of borane research and unlock new possibilities in chemistry, materials science, and beyond.
To work with boranes, researchers may utilize solvents like DMSO, formic acid, iodomethane, and acetonitrile.
Additives like PNGase F, sodium hydroxide beads, acetic acid, and methanol may also be employed, depending on the specific application.
By understanding the properties and reactivity of boranes, scientists can develop innovative solutions to complex problems and advance the field of borane research.
These unique molecules have a wide range of applications in chemistry, materials science, and energy research.
BH3 compounds, also known as borane or borohydride, exhibit distinctive structural and reactivity properties, making them valuable tools for synthetic transformations, catalysis, and the development of novel materials.
Boranes can be used in a variety of applications, including as reducing agents, in the synthesis of other boron-containing compounds, and in the production of advanced materials.
Related terms include diborane (B2H6), trimethylborane (B(CH3)3), and ammonia borane (NH3BH3), which is a promising hydrogen storage material.
PubCompare.ai leverages advanced AI algorithms to help researchers identify the most accurate and reproducible protocols for working with boranes, enabling reliable, high-quality results in their scientific endeavors.
By exploring the power of AI-driven protocol comparison, researchers can discover the future of borane research and unlock new possibilities in chemistry, materials science, and beyond.
To work with boranes, researchers may utilize solvents like DMSO, formic acid, iodomethane, and acetonitrile.
Additives like PNGase F, sodium hydroxide beads, acetic acid, and methanol may also be employed, depending on the specific application.
By understanding the properties and reactivity of boranes, scientists can develop innovative solutions to complex problems and advance the field of borane research.