Total RNAs were extracted using standard hot phenol RNA preparation method (51 (link)). For RT-PCR analysis, 1.5 µg of total RNAs were subjected to reverse transcription (RT) using gene-specific primers, and then the resulting cDNAs were analysed by standard PCR method or qPCR. Primers used are listed in Supplementary Table S3 . The half-life of pre-mRNAs was determined as described previously (52 (link),53 (link)). For northern blotting, the procedures were as previously described (50 (link)) except that total RNAs (10 µg) were separated by 6% acrylamide gels. Sequences of oligonucleotide probes are listed in Supplementary Table S3 . Band intensities were quantified using ImageJ densitometry software.
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Acrylamide
Acrylamide
Acrylamide is a chemical compound with the formula CH2=CHCONH2.
It is a colorless, odorless, crystalline solid that is highly solubike in water and other polar solvents.
Acrylamide has a wide range of industrial and scientific applications, including the production of polyacrylamide, which is used in water treatment, papermaking, and other industries.
In the human body, acrylamide can be formed during the cooking of certain foods, particularly at high temperatures, and has been associated with potential health risks.
Researchers studying acrylamide may use PubCompare.ai to optimize their research by identifying the best protocols from literature, preprints, and patents, and leverageing AI-powered comparisons to enhance reproducibility and accuracy in their work.
It is a colorless, odorless, crystalline solid that is highly solubike in water and other polar solvents.
Acrylamide has a wide range of industrial and scientific applications, including the production of polyacrylamide, which is used in water treatment, papermaking, and other industries.
In the human body, acrylamide can be formed during the cooking of certain foods, particularly at high temperatures, and has been associated with potential health risks.
Researchers studying acrylamide may use PubCompare.ai to optimize their research by identifying the best protocols from literature, preprints, and patents, and leverageing AI-powered comparisons to enhance reproducibility and accuracy in their work.
Most cited protocols related to «Acrylamide»
Acrylamide
Densitometry
DNA, Complementary
Gels
Genes
mRNA Precursor
Oligonucleotide Primers
Oligonucleotide Probes
Phenol
Reverse Transcription
RNA
Standard Preparations
Acid Hybridizations, Nucleic
Acrylamide
Antibodies
Buffers
Equus asinus
Immunoglobulins
Immunoglobulins, Fab
Nitrogen
paraform
PEGDMA Hydrogel
Phosphates
Propane
RBBP8 protein, human
Serum
Sodium Azide
Tissues
For most routine extractions of fungal tissue and our early extractions of ectomycorrhizal root tips, DNA was extracted using a CTAB protocol with 0.8% mercaptoethanol and incubating at 65°C for 1 hour [34 (link)]. An alternate extraction method modified from Ross [35 (link)] based on the action of xanthogenate [36 ] was later employed for rapid survey analyses of large numbers of ectomycorrhizal root tips. Single ectomycorrhizal root tips (approximately 1 mg tissue dry weight) were placed in each 1.5 mL tube with 600 μL Xanthine/Tween Buffer (100 mM Tris-HCl pH 7.5, 12.5 mM potassium ethyl xanthogenate (Fluka, Buchs, Switzerland, cat. no. 60040), 10 mM EDTA pH 8.0, 10% Tween 20, 500 mM NaCl). These were sonicated for 15 seconds and incubated 90–120 min at 60°C on a shaker. Tubes were then centrifuged 5 min at 10,000 × g to pellet undissolved tissue. Supernatant was removed to a new tube containing 1/5 volume of PEG/NaCl (20% PEG-8000/2.5 M NaCl [37 (link)]) and 8 μL 1.25 mg/mL Linear Acrylamide (Ambion, Austin, Texas, cat. no. 9520). This was mixed by gently tipping the tube, and the mixture was incubated at 30°C for 15 minutes. DNA was precipitated by centrifuging 5 minutes (room temp.) at 10,000 × g, and then recovered by removing the PEG solution. The final DNA pellet was obtained by rinsing twice with 200 μL 80% ice-cold ethanol while mixing by gently tipping the tubes, followed by centrifuging 5 minutes at 10,000 × g, and lastly, by drying the pellet under vacuum for 30–45 minutes. In both protocols DNA was resuspended in a final volume of 100 μL TE buffer (pH 8, 10 mM Tris: 1 mM EDTA) after extraction by heating to 37°C for 10 minutes.
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2-Mercaptoethanol
Acrylamide
austin
Buffers
Cetrimonium Bromide
Cold Temperature
Ectomycorrhizae
Edetic Acid
Ethanol
Neoplasm Metastasis
polyethylene glycol 8000
potassium ethylxanthate
Root Tip
Sodium Chloride
Tissues
Tromethamine
Tween 20
Tweens
Vacuum
Xanthine
Acrylamide
Biological Assay
Deoxyribonuclease EcoRI
Embryo
Ethidium Bromide
Genes
Genome
MECP2 protein, human
Mus
Mutation
Oligonucleotide Primers
Restriction Fragment Length Polymorphism
Sepharose
Acrylamide
Biological Assay
Biopsy
Embryonic Stem Cells
Ethidium Bromide
Genome
Mus
Mutation
Oligonucleotide Primers
Restriction Fragment Length Polymorphism
Sepharose
Tail
Most recents protocols related to «Acrylamide»
Example 161
To a solution of 2-(piperazin-1-yl)ethanol (0.73 g, 5.6 mmol, 1 eq.) in DMF (10 mL) was added K2CO3 (1.56 g, 11.3 mmol, 2 eq.) followed by 1,2,4-trifluoro-5-nitrobenzene (1 g, 5.6 mmol, 1 eq.) and the mixture was stirred at 0° C. for 1 hour. The mixture was poured into ice-water (100 mL), extracted by EA (3×40 mL), and the organic layers were combined, washed with brine (150 mL), concentrated and purified via column chromatography (10-95% CH3CN—H2O) to afford 2-(4-(2,5-difluoro-4-nitrophenyl)piperazin-1-yl)ethanol (0.65 g, 41%) as a yellow solid.
To a solution of 2-(4-(2,5-difluoro-4-nitrophenyl)piperazin-1-yl)ethanol (0.65 g, 2.3 mmol) in MeOH (50 mL) was added Pd/C (100 mg) and the resulting mixture was stirred at r.t. overnight. The Pd/C was removed by filtration and the filtrate was concentrated to afford 2-(4-(4-amino-2,5-difluorophenyl)piperazin-1-yl)ethanol (0.58 g, 99%).
To a suspension of 2-(4-(4-amino-2,5-difluorophenyl)piperazin-1-yl)ethanol (270 mg, 0.88 mmol, 1 eq.) and N-(3-(2-chloroquinazolin-8-yl)phenyl)acrylamide (225 mg, 0.88 mmol, 1 eq.) in n-BuOH (10 mL) was added TFA (0.5 mL, 4.4 mmol, 5 eq.) and the resulting mixture was stirred at 90° C. overnight. The mixture was concentrated, diluted with DCM (20 mL), washed with Na2CO3 solution (20 mL), dried, concentrated and purified via column chromatography (DCM/MeOH=10/1) to afford N-(3-(2-((2,5-difluoro-4-(4-(2-hydroxyethyl)piperazin-1-yl)phenyl)amino)quinazolin-8-yl)phenyl)acrylamide (120 mg, 26%) as yellow solid. LRMS (M+H+) m/z calculated 531.2, found 531.2. 1H NMR (DMSO-d6, 400 MHz) δ 10.18 (s, 1H), 9.37 (s, 1H), 9.17 (s, 1H), 7.97-7.94 (m, 3H), 7.83-7.74 (m, 2H), 7.50-7.39 (m, 3H), 6.90-6.85 (m, 1H), 6.48-6.41 (m, 1H), 6.23 (dd, 1H), 5.73 (dd, 1H), 4.42 (t, 1H), 3.55-3.50 (m, 2H), 2.94-2.91 (m, 4H), 2.55-2.54 (m, 4H), 2.44 (t, 2H).
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1H NMR
Acrylamide
brine
Chromatography
Ethanol
Filtration
Ice
Nitrobenzenes
Piperazine
potassium carbonate
Sulfoxide, Dimethyl
Example 82
N-(3-(2-((4-(2-oxooxazolidin-3-yl)phenyl)amino)quinazolin-8-yl)phenyl)acrylamide (71.0 mg) was prepared as described for (S)—N-(3-(2-((4-((1-acetylpyrrolidin-3-yl)oxy)phenyl)amino)quinazolin-8-yl)phenyl)acrylamide. LRMS (M+H+) m/z calculated 452.2, found 452.1. 1H NMR (DMSO-d6, 400 MHz) δ10.30 (s, 1H), 9.95 (s, 1H), 9.36 (s, 1H), 8.06 (s, 1H), 7.81-7.96 (m, 5H), 7.48 (q, 2H), 7.41 (d, 1H), 7.29 (d, 2H), 6.43-6.47 (m, 1H), 6.26 (d, 1H), 5.75 (d, 1H), 4.42 (t, 2H), 3.97 (t, 2H).
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1H NMR
Acrylamide
Sulfoxide, Dimethyl
Example 58
N-(3-(2-((4-(4-(2,2-difluoroethyl)piperazin-1-yl)phenyl)amino)quinazolin-8-yl)phenyl)acrylamide (74.2 mg) was prepared as described for (S)—N-(3-(2-((4-((1-acetylpyrrolidin-3-yl)oxy)phenyl)amino)quinazolin-8-yl)phenyl)acrylamide. LRMS (M+H+) m/z calculated 515.2, found 515.2. 1H NMR (CD3OD, 300 MHz) δ 9.15 (s, 1H), 7.52-7.99 (m, 6H), 7.39-7.50 (m, 3H), 6.80 (d, 2H), 6.41-6.52 (m, 2H), 6.02 (t, 1H), 5.77-5.84 (m, 1H), 3.03-3.12 (m, 4H), 2.74-2.88 (m, 6H).
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1H NMR
Acrylamide
Piperazine
Example 72
N-(3-(2-((4-(3-(hydroxymethyl)-4-methylpiperazin-1-yl)phenyl)amino)quinazolin-8-yl)phenyl)acrylamide (41.4 mg) was prepared as described for (S)—N-(3-(2-((4-((1-acetylpyrrolidin-3-yl)oxy)phenyl)amino)quinazolin-8-yl)phenyl)acrylamide. LRMS (M+H+) m/z calculated 495.2, found 495.2. 1H NMR (CD3OD, 400 MHz) δ 9.01 (s, 1H), 7.80-7.86 (m, 2H), 7.68 (d, 2H), 7.62 (d, 2H), 7.35-7.38 (m, 1H), 7.25-7.30 (m, 2H), 6.68 (d, 2H), 6.25-6.39 (m, 2H), 5.68 (d, 1H), 3.66-3.67 (m, 2H), 3.45 (d, 1H), 3.33 (d, 1H), 2.99 (d, 1H), 2.56-2.71 (m, 1H), 2.46 (s, 1H)
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1H NMR
Acrylamide
Example 106
(R)—N-(3-(2-((4-((tetrahydrofuran-3-yl)oxy)phenyl)amino)quinazolin-8-yl)phenyl)acrylamide (55.0 mg) was prepared as described for (S)—N-(3-(2-((4-((1-acetylpyrrolidin-3-yl)oxy)phenyl)amino)quinazolin-8-yl)phenyl)acrylamide. LRMS (M+H+) m/z calculated 453.2, found 453.1. 1H NMR (DMSO-d6, 400 MHz) δ 10.29 (s, 1H), 9.76 (s, 1H), 9.31 (s, 1H), 8.04 (s, 1H), 7.78-7.93 (m, 5H), 7.33-7.50 (m, 3H), 6.66 (d, 2H), 6.44-6.50 (m, 1H), 6.23-6.28 (m, 1H), 5.74-5.77 (m, 1H), 4.85-4.87 (m, 1H), 3.37-3.86 (m, 4H), 2.12-2.17 (m, 1H), 1.89-1.91 (m, 1H).
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1H NMR
Acrylamide
Sulfoxide, Dimethyl
tetrahydrofuran
Top products related to «Acrylamide»
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Acrylamide is a chemical compound used in the production of various laboratory equipment and materials. It is a white, crystalline solid with a high water solubility. Acrylamide is commonly utilized in the manufacture of polyacrylamide gels, which are widely used in electrophoresis techniques for the separation and analysis of biological macromolecules, such as proteins and nucleic acids.
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PVDF membranes are a type of laboratory equipment used for a variety of applications. They are made from polyvinylidene fluoride (PVDF), a durable and chemically resistant material. PVDF membranes are known for their high mechanical strength, thermal stability, and resistance to a wide range of chemicals. They are commonly used in various filtration, separation, and analysis processes in scientific and research settings.
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Ammonium persulfate is a white crystalline chemical compound that is commonly used as an initiator in various chemical reactions, particularly in the field of polymerization. It serves as an oxidizing agent and is known for its ability to generate free radicals, which are essential for initiating and accelerating polymerization processes.
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Acrylamide is a chemical compound commonly used as a reagent in electrophoresis, a laboratory technique for the separation and analysis of biomolecules such as proteins and nucleic acids. It serves as a key component in the preparation of polyacrylamide gel, which is a versatile matrix used in various electrophoresis applications.
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Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
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Image Lab software is a data analysis tool designed for use with Bio-Rad's gel and blot imaging systems. The software provides a user-friendly interface for capturing, analyzing, and processing images of gels, blots, and other samples.
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Nitrocellulose membranes are a type of laboratory equipment designed for use in protein detection and analysis techniques. These membranes serve as a support matrix for the immobilization of proteins, enabling various downstream applications such as Western blotting, dot blotting, and immunodetection.
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The Protease Inhibitor Cocktail is a laboratory product designed to inhibit the activity of proteases, which are enzymes that can degrade proteins. It is a combination of various chemical compounds that work to prevent the breakdown of proteins in biological samples, allowing for more accurate analysis and preservation of protein integrity.
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N,N′-methylenebisacrylamide is a chemical compound used as a cross-linking agent in various laboratory applications. It is a white crystalline solid that is soluble in water and organic solvents.
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Sodium dodecyl sulfate (SDS) is a commonly used anionic detergent for various laboratory applications. It is a white, crystalline powder that has the ability to denature proteins by disrupting non-covalent bonds. SDS is widely used in biochemical and molecular biology techniques, such as protein electrophoresis, Western blotting, and cell lysis.
More about "Acrylamide"
Acrylamide is a chemical compound with the formula CH2=CHCONH2, also known as propenamide or acrylic amide.
It is a colorless, odorless, crystalline solid that is highly soluble in water and other polar solvents.
Acrylamide has a wide range of industrial and scientific applications, including the production of polyacrylamide, which is used in water treatment, papermaking, and other industries.
In the human body, acrylamide can be formed during the cooking of certain foods, particularly at high temperatures, and has been associated with potential health risks.
Researchers studying acrylamide may also use related compounds and materials such as PVDF membranes, ammonium persulfate, bovine serum albumin, Image Lab software, nitrocellulose membranes, protease inhibitor cocktails, N,N'-methylenebisacrylamide, and sodium dodecyl sulfate in their experiments and analyses.
PubCompare.ai is an AI-driven platform that can help optimize acrylamide research by identifying the best protocols from literature, preprints, and patents, and leveraging AI-powered comparisons to enhance reproducibility and accuracy.
By utilizing this tool, researchers can discover the optimal methods and products to advance their acrylamide-related discoveries and investigations.
It is a colorless, odorless, crystalline solid that is highly soluble in water and other polar solvents.
Acrylamide has a wide range of industrial and scientific applications, including the production of polyacrylamide, which is used in water treatment, papermaking, and other industries.
In the human body, acrylamide can be formed during the cooking of certain foods, particularly at high temperatures, and has been associated with potential health risks.
Researchers studying acrylamide may also use related compounds and materials such as PVDF membranes, ammonium persulfate, bovine serum albumin, Image Lab software, nitrocellulose membranes, protease inhibitor cocktails, N,N'-methylenebisacrylamide, and sodium dodecyl sulfate in their experiments and analyses.
PubCompare.ai is an AI-driven platform that can help optimize acrylamide research by identifying the best protocols from literature, preprints, and patents, and leveraging AI-powered comparisons to enhance reproducibility and accuracy.
By utilizing this tool, researchers can discover the optimal methods and products to advance their acrylamide-related discoveries and investigations.