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Benzochloryl

Benzochloryls are a class of organic compounds featuring a benzene ring fused to a chlorinated heterocyclic ring.
These versatile molecules find applications in pharmaceutical development, materials science, and various research fields.
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Most cited protocols related to «Benzochloryl»

We used two colonies of Anopheles gambiae mosquitoes: the DDT-resistant ZAN/U colony with increased metabolism of the insecticide, catalyzed by members of the glutathione S-transferees enzyme family [13] (link) and the DDT-sensitive Kisumu colony from western Kenya [14] (link). Two-day old larvae were transferred to 12-well plates and reared individually in 3 ml of de-ionized water. For each colony we reared 480 mosquitoes at each of three feeding regimes: 100% (high), 70% (medium) or 40% (low) of the standard amount of TetraMin® Baby fish food (Table 1). Given food quantities were administered to each well in 100 uL of de-ionized water (which partially compensates for evaporative loss). Emerged females were moved to plastic cups and supplied with cotton balls moistened with saturated 10% sugar solution, males were discarded.
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Publication 2013
Anopheles gambiae Benzochloryl Carbohydrates Culicidae Enzymes Females Fishes Food Glutathione Gossypium Infant Food Larva Males Metabolism
Anopheline mosquito larvae were collected by dipping from a range of breeding sites (road puddies, brick pits, pools, marshes, streams, surface water harvest, ditches, dam reservoir shore, pits dug for plastering traditional tukuls, and pits dug for pot making) in the two groups of villages (‘low risk’ and ‘high risk’) during the wet season from July to September 2009. The larvae were reared to adults in the field laboratory at Asendabo Health Centre, southwestern Ethiopia, under standard conditions (25±2°C, 80%±4% (Relative Humidity). Two to three days old, non blood-fed female mosquitoes were selected and exposed to insecticide impregnated papers with discriminating concentrations of DDT (4%), permethrin (0.75%), deltamethrin (0.05%), malathion (5%) and propoxur (0.1%) using WHO standard assays [33] . The insecticide impregnated and control papers were obtained from the WHO collaboration Centre, Vector Control Research Unit, School of Biological Sciences, Penang, Malaysia. Batches of 20–24 mosquitoes in five replicates were exposed in test kit tubes for all bioassays for one hour against the four classes of insecticides and knockdown was recorded at 10, 15, 20, 30, 40, 50, and 60 minutes. Equal numbers of mosquitoes were exposed to the corresponding control papers impregnated with resila oil (Organochlorine control), olive oil (Organophosphate/Carbamate control), and silicone oil (Pyrethroid control). After one hour, mosquitoes were transferred into holding tubes and provided 10% sucrose solution with cotton pads. Mortality was recorded after 24 hours of exposure. Likewise, a strain of An. arabiensis from Malaria Training Centre, Nazareth, Ethiopia that has been maintained in the laboratory without exposure to insecticides for over 30 years was exposed to the insecticide papers as reference. The identification of mosquitoes was conducted morphologically as An. gambiae s.l using a standard key [34] . Mosquitoes both dead and alive were individually preserved in Eppendorf tubes over silica-gel for further molecular assays.
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Publication 2011
Adult Benzochloryl Biological Assay BLOOD Carbamates Culicidae decamethrin Females Gossypium Humidity Insecticides Larva Malaria Malathion Marshes Oil, Olive Organophosphates Permethrin Propoxur Pyrethroids Silica Gel Silicone Oils Strains Sucrose Van der Woude syndrome
In 2000–2001, we measured DDT-related compounds in serum samples that had been collected during 1959–1967. The mean age of subjects when blood was drawn was 26 years. Samples collected within 1–3 days of delivery were used for 82% of the cases and 86% of controls, and serum drawn during the third trimester was used for the remainder. Longnecker et al. (1999) (link), reported that organochlorine levels assayed across all trimesters of pregnancy and soon after delivery were highly correlated, indicating that the time when samples are collected over the 9 months of pregnancy is not critical. We stored serum samples at −20°C. Samples were first thawed to prepare an aliquot of 1.5 mL for organochlorine assays. The aliquots were shipped frozen to the laboratory; p,p-DDE, o,p-DDT, and p,p-DDT were assayed using methods reported previously by Gammon et al. (2002) (link). Sample order was randomly assigned within and across batches. Case–control pairs were analyzed in the same batches to minimize differences due to laboratory drift. The laboratory was blind as to case or control status of the samples. As described previously by Berkowitz et al. (2003) (link), we used all observed positive values of o,p-DDT in analyses, even those reported to be below the limit of detection (LOD); seven o,p-DDT measurements with reported negative values were recoded as the lowest measured value (i.e., 0.01 μg/L). Interbatch and intrabatch coefficients of variation were 16% and 5%, respectively, for p,p-DDT; 11% and 4% for p,p-DDE; and 26% and 5% for o,p-DDT. Total cholesterol and total triglycerides were measured enzymatically on a Hitachi 911 analyzer (Roche Diagnostics, Indianapolis, IN) in a laboratory certified by the Centers for Disease Control and Prevention (Atlanta, GA) and the National Heart Lung and Blood Institute Lipid Standardization Program (Bethesda, MD).
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Publication 2007
Benzochloryl Blindness BLOOD Cholesterol Diagnosis Freezing Heart Lipids Lung o,p'-DDT Obstetric Delivery Pregnancy Serum Triglycerides
After obtaining information on the study and signing an informed consent, participants completed a detailed and validated Danish questionnaire with the assistance of a qualified interviewer during a face-to-face interview. The questionnaire was adapted from the Mediterranean Osteoporosis Study Questionnaire (MEDOS) [27 (link)] and allowed to document the following risk factors for osteoporosis: smoking habits, physical activity, daily milk products and calcium supplement consumption, current use of HRT and former contraceptive use. Questions were also asked to document secondary causes of osteoporosis such as Cushing's disease, renal and liver deficiency, hyper- and hypothyroidism, bone cancer and rheumatoid arthritis.
Weight, height and waist, abdominal and hip girth were measured using standardized techniques. Women were considered postmenopausal if they had no menses for at least one year before the recruitment, if they underwent bilateral oophorectomy more than 6 months ago and if analysis had revealed a follicle stimulating hormone concentration greater than 40 IU/L.
Blood samples (10 ml) were collected in vials containing EDTA as the anticoagulant, centrifuged (10 min, 3000 rpm) and the plasma poured into glass vials pre-rinsed with hexane. First morning urine samples were collected in plastic vials. Plasma and urine samples were stored frozen at -80°C until time of analysis for OCs and cadmium respectively, at the Centre de Toxicologie (CTQ) of the Institut National de Santé Publique du Québec (Québec, Canada). This laboratory is accredited by the Canadian Association for Environmental Analytical Laboratories.
Fourteen PCB congeners [International Union for Pure and Applied Chemistry (IUPAC) no. 28, 52, 99, 101, 105, 118, 128, 138, 153, 156, 170, 180, 183, 187], p, p'-DDT (dichlorodiphenyltrichloroethane) and its major metabolite p, p'-DDE (p, p'-dichlorodiphenyl-dichloroethylene), hexachlorobenzene (HCB) and 8 other chlorinated pesticides [α-chlordane, γ-chlordane, aldrin, β-hexachlorocyclohexane (β-HCH), mirex, oxychlordane, cis-nonachlor, and trans-nonachlor] were analysed by high-resolution gas chromatography with electron capture detection. A 1:1:3 mixture of ammonium sulfate:ethanol:hexane was first added to the plasma to extract OCs. The extracts were then concentrated and purified on two Florosil columns (60–100 mesh; Fisher Scientific, Nepean, Ontario, Canada). The OCs were measured in the purified extracts with an HP 5890 high-resolution gas chromatograph equipped with dual-capillary columns (HP Ultra I and Ultra II) and dual Ni-63 electron capture detectors (Hewlett-Packard, Palo Alto, CA, USA). The limit of detection was based on 3 times the average standard deviation of noise and was 0.08 μg/L for p, p'-DDE, p, p'-DDT and β-HCH, and 0.04 μg/L for all other compounds. The average percent recoveries were greater than 95%. Coefficients of variations (CVs) based on repeated analyses of a standard reference material (SRM 1589; N = 15) ranged from 3.9% to 18.5%, except for PCB 105 and p, p'-DDT, for which CVs were 31.6% and 26.2%, respectively.
Plasma OC concentrations were expressed on a lipid basis. We calculated the total plasma lipid concentration from the concentrations of cholesterol esters, free cholesterol, triglycerides and phospholipids [28 (link)], which were measured using standard enzymatic procedures.
Urine samples were analysed for cadmium by graphite furnace atomic absorption spectrometry and concentrations were corrected for urinary creatinine content to take into account differences in urinary output between participants. Duplicates were run every 10 samples. The CV of the method based on repeated analyses of a standard reference material from CTQ interlaboratory comparison program (N = 34) was 2.0%, and the limit of detection was 0.2 μg/L.
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Publication 2006
Abdomen Aldrin Anticoagulants Benzochloryl BLOOD Bone Cancer Cadmium Calcium, Dietary Capillaries Chlordan Cholesterol Cholesterol Esters Contraceptive Agents Creatinine Cushing's Disease Dietary Supplements Edetic Acid Electrons Enzymes Ethanol Face Female Castrations Follicle-stimulating hormone Freezing Gas Chromatography Graphite Hexachlorobenzene Hexanes Hypothyroidism Interviewers Kidney Lindane Lipid A Lipids Liver Menstruation Milk Mirex nonachlor nonachlor, (trans)-isomer Osteoporosis oxychlordane PCB 105 Pesticides Phospholipids Plasma Rheumatoid Arthritis Spectrophotometry, Atomic Absorption Sulfate, Ammonium Triglycerides Urine Woman
Indoor female Anopheles mosquitoes were collected in the village of Palmeira (25° 15′ 19′′ S; 32° 52′ 22′′ E), Manhiça district, Maputo province (southern Mozambique) near the Incomati river. The majority of inhabitants are farmers (sugar cane, rice) from the Xichangana and Xironga communities. The collections were performed during 4–5 days in 3 consecutive years (August 2016, April 2017, and January 2018) using electric aspirators. Anopheles funestus s.s. is the primary malaria vector in this area [27 (link)]. An Anopheles funestus sample collected in 2002 [28 (link)] was used for comparative genotyping of the CYP6P9a resistance allele. Most of the households have LLINs (Olyset and PermaNet 2.0) impregnated only with pyrethroids, whereas IRS with dichlorodiphenyltrichloroethane (DDT) is also applied [27 (link)].
Collected Anopheles female mosquitoes (blood-fed, gravid and half-gravid) were morphologically identified as belonging to An. funestus group or An. gambiae complex according to morphological keys [29 ]. Anopheles funestus sensu lato (s.l.) females were kept in cages until they became fully gravid, and subsequently, forced to lay eggs in separate 1.5-mL microcentrifuge tubes and larvae reared to adults as previously described [30 (link)]. Seventy An. funestus s.l. females collected in April 2017 were bisected into head plus thorax and abdomen and kept individually. Genomic DNA (gDNA) from these mosquitoes were extracted using the Livak method [31 (link)], followed by a cocktail polymerase chain reaction (PCR) as previously described [32 (link)] for species identification with An. funestus group. The internal transcribed spacer 2 (ITS2) was sequenced for samples that failed to amplify.
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Publication 2019
Abdomen Adult Alleles Anopheles Benzochloryl BLOOD Chest Cloning Vectors Culicidae Eggs Electricity Farmers Females Genome Head Households Larva Malaria Oryza sativa Polymerase Chain Reaction Pyrethroids Rivers Saccharum Woman

Most recents protocols related to «Benzochloryl»

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Publication 2023
Benzochloryl cypermethrin decamethrin Dichlorodiphenyl Dichloroethylene Dieldrin Endosulfan Epoxy Compounds Ethyl Ether Florisil Heptachlor High-Performance Liquid Chromatographies Methanol Methoxychlor n-hexane o,p'-DDT Ocular Cicatricial Pemphigoid Permethrin Pesticides Pyrethroids Solvents
Extraction of proteins: An analysis of three biological replicates was processed in both ways with proteomics. The method of extracting protein from the RJ and MRJPs for proteomic analysis was described by previous reports, with some modifications [17 (link)]. Briefly, the RJ and MRJPs were each mixed with a Lysis buffer (1:1, v/v) and ultrasonicated for 2 min using an ultrasonic cell crusher at 4 °C. The above mixtures were allowed to centrifuge at 12,000× g rpm at 4 °C for 20 min, and the upper supernatant was mixed with three times the volume of cold acetone to precipitate the remaining proteins. This centrifugal operation was repeated three times. The supernatant was then removed, and the precipitate was collected. Then, 100 µL of 5 M urea was mixed into the precipitate, followed by ultrasonic treatment. Finally, 400 µL of NH4CO3 (40 mM) was mixed into the above solution.
Protein concentration determination: The method of determining the protein concentration referred to the previously reported study [18 (link)]. After the samples were diluted, the BCA kit was used to detect the protein concentration of the samples.
Proteolytic cleavage: An amount of 400 µg of RJ and MRJP protein extracts were mixed with 4 mL of DDT solution (0.1 M). After 1 h of reaction at chamber temperature, 2 mL of DDT (Dichlorodiphenyltrichloroethane) solution was added to the above mixture incubated in the dark for 1 h. Then, a trypsin enzyme with one-fifth of the protein mass was added for overnight cleavage. After 14 h, 1 µL of formic acid was mixed in to terminate the cleavage.
Peptide desalting and quantification: Desalting was performed using ZipTipC18 pipette tips, according to the standard procedure in the user guide [19 (link)].
The desiccated samples were solubilized in 0.1% formic acid water and centrifuged at 12,000× g rpm under chamber temperature for 20 min. After taking the supernatant, the peptide concentration was determined using a Nanodrop. It was then diluted to 0.25 µg/µL for mass spectrometry.
Mass spectrometry analysis:A peptide analysis was carried out using an HPLC system Easy-nLC1200 from Thermo Fisher Scientific (Waltham, MA, USA). This was coupled to a Thermo Orbitrap QE-HF mass spectrometer, according to previous reports [20 (link)].
Database searching and validation: Raw MS files were processed using MaxQuant, an in-house software suite developed in [21 (link)]. The search engine used was Andromeda, the search database used was Apis mellifera (from NCBI), and the contamination library used was the common contamination library sequence from MaxQuant software (version 1.6.1.0, Max-Planck-Institute of Biochemistry, Martinsried, Germany).
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Publication 2023
Acetone Apis Benzochloryl Biopharmaceuticals Buffers cDNA Library Cells Cold Temperature Cytokinesis Enzymes formic acid High-Performance Liquid Chromatographies Mass Spectrometry Peptides Proteins Proteolysis Staphylococcal Protein A Trypsin Ultrasonics Urea
Polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCs) were analyzed through gas chromatography coupled with tandem mass spectrometry (GC-MS/MS). Forty-one PCBs (CB-17, 18, 28, 31, 33, 44, 49, 52, 70, 74, 82, 87, 95, 99, 101, 105, 110, 118, 128, 132, 138, 149, 151, 153, 156, 158, 169, 170, 171, 177, 180, 183, 187, 191, 194, 195, 199, 205, 206, 208 and 209) and eighteen OCPs (α-HCH, β-HCH, δ-HCH, γ-HCH, Heptachlor, Aldrin, Heptachlor epoxide, Endossulfan-I, p-p’DDE, Dieldrin, Endrin, Endossulfan-II, p-p’DDD, Endrin aldeide, Endossulfan sulphate, p-p’DDT, Endrin cetone and Metoxychlor) were determined using certified standards (AccuStandard ®, Quebec Ministry of Environment Congener Mix (C-QME-01) and Pesticide Mix (Z-014C-R), respectively).
Standard working mixtures of all compounds were prepared from stock solutions by dilution to obtain the following concentrations: 0.50, 1.0, 2.0, 5.0, 8.0, 10, 15, 20 and 25 ng m L−1. The analyses were performed using a Thermo Scientific Trace GC Ultra model coupled to a Thermo Scientific TSQ Quantum XLS tandem mass spectrometer. Chromatographic conditions are detailed in Table 1.
The GC-MS/MS analysis, using a triple-quadrupole analyzer (QqQ), was performed in the selective reaction monitoring mode (SRM). This method results in higher selectivity than traditional mass spectrometry, as it monitors the fragmentation pattern between the precursor ion (Q1) and the product ion (Q3) obtained in the second quadrupole (q) [28 (link),29 (link),30 (link)]. The GC-MS/MS method is widely applied to samples containing low compound concentrations and to complex matrices, as it reduces spectral interferences being, therefore, more selective [30 (link)]. The triple quadruple fragmentation patterns (two precursor-products for each analyte) used for identification and quantification are detailed in Table 2. The method limit of quantification (LOQ) corresponds to ten times the standard deviation of background signal to noise of the lowest level of the curve. The LOQ ranged from 0.12 to 0.38 ng g−1.
The PCBs, Pesticides, surrogates and internal standards were obtained from Accustandard ® (New Haven, CT, USA). Sorbent materials used for column chromatography comprised silica gel 60 (0.063–0.200 mm) (CAS-No 112926-00-8; Supelco, Saint Louis, MO, USA) and aluminum oxide 90 active neutral (CAS-No 1344-28-1; Supelco, Saint Louis, USA), both acquired from Merck (Rio de Janeiro, Brazil). Sodium sulfate (CAS-No 7757-82-6) was acquired from Sigma-Aldrich (Saint Louis, USA).
Method precision was estimated based on the Residual Standard Deviation (RSD) 20% obtained by analyzing seven replicate samples fortified with 5 ng of all targeted compounds (RSD—9 ± 6 %). Samples of the IAEA-435 certified material—Tuna Homogenate—were extracted and quantified as part of the analytical control process. The evaluation of IAEA-435 results was performed through normalized error (equation 1), a relation between the mean and uncertainty of the certified reference material ( x¯1±u1 ) and the achieved results ( x¯2±u2 ). Normalized error values lower than 1 are considered conforming or passing, and outside of this value (≥1) are considered nonconforming or failing. All values for normalized error in this research were within an acceptable range (<1; 0.61 ± 0.17).
|x¯1x¯2|u12+u22
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Publication 2023
Aldrin Benzochloryl Chromatography compound NG Dichlorodiphenyl Dichloroethylene Dieldrin Endrin Gas Chromatography Heptachlor Heptachlor Epoxide Mass Spectrometry Ocular Cicatricial Pemphigoid Oxide, Aluminum Pesticides Polychlorinated Biphenyls Silica Gel sodium sulfate Sulfates, Inorganic Tandem Mass Spectrometry Technique, Dilution Tuna
The mixed standard samples of organochlorine pesticides were purchased from China Institute of Metrology. The standard substance used high-purity isooctane/high-purity toluene (mass ratio: 99.7/0.3) as the solvent, and α-Hexachlorocyclohexane (α-HCH), δ-Hexachlorocyclohexane (δ-HCH), γ-Hexachlorocyclohexane (γ-HCH), δ-Hexachlorocyclohexane (δ-HCH), o, p′-Dichlorodiphenyltrichloroethane (o, p′-DDT), p, p′-Dichlorodiphenyltrichloroethane (p, p′-DDT), p, p′- Dichlorodiphenyldichloroethane (p, p′-DDD), p, p′-Dichlorodiphenylethane (p, p′-DDE), hexachlorobenzene, heptachlor, heptachlor epoxide, aldrin, dieldrin, endrin, and mirex, a total of 15 organochlorine pesticides. The standard solution was prepared into 0, 5, 10, 20, and 50 μg/L standard solutions with n-hexane, and a standard curve was established. The correlation coefficients of the established standard curves were all between 0.938–0.999, which meets the requirements. The linear regression equation and fitting degree are given in the Supplementary Table S2.
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Publication 2023
2,2,4-trimethylpentane Aldrin Benzochloryl Dichlorodiphenyldichloroethane Dieldrin Endrin Heptachlor Heptachlor Epoxide Hexachlorobenzene Lindane Mirex n-hexane Pesticides Solvents Toluene
The chemicals and reagents used for this study were as follows: analytical grade n-hexane, acetone, acetonitrile, methanol, glacial acetic acid, and for cleanup purposes, anhydrous magnesium sulfate, sodium acetate, and florisil, and for centrifugation, 50 ml and 15 ml centrifuge tubes were used. Commonly studied pesticides by different scholars in Ethiopia were used to be studied in this study, which include o,p'-DDT, p,p'-DDT, p,p'-DDE, p,p'-DDD, cypermethrin, and deltamethrin, which were identified from different food matrices and water by [11 (link), 18 (link), 19 (link)] and expected to be detected in commonly consumed vegetables. In addition, other pesticides were selected based on the availability of their standards. Therefore, eight organochlorine pesticides and metabolites including o,p'-DDT, p,p'-DDT, p,p'-DDE, p,p'-DDD, γ-chlordane, dimethachlor, lindane, and heptachlor and two pyrethroids (cypermethrin and deltamethrin) were selected for this study. The analytical purity of those pesticide standards, extraction solvents, and salts was greater than 98%.
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Publication 2023
Acetic Acid Acetone acetonitrile Benzochloryl Centrifugation Chlordan cypermethrin decamethrin Dichlorodiphenyl Dichloroethylene Florisil Food Heptachlor Hexanes Lindane Methanol o,p'-DDT Pesticides Pyrethroids Salts Sodium Acetate Solvents Sulfate, Magnesium Vegetables

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Permethrin is a synthetic pyrethroid compound commonly used as an insecticide and acaricide. It acts as a contact and stomach poison for a wide range of insects and arthropods. Permethrin is primarily used in agricultural, industrial, and public health applications to control pests.
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More about "Benzochloryl"

Benzochloryl is a class of organic compounds featuring a benzene ring fused to a chlorinated heterocyclic ring.
These versatile molecules have a wide range of applications in the pharmaceutical, materials science, and research fields.
Closely related to Benzochloryl are other halogenated organic compounds like Acetonitrile, Permethrin, and Chlorpyrifos.
These compounds often share similar chemical structures and properties, making them useful in various research and industrial applications.
Solvents like Acetone, N-hexane, and DMSO are commonly used in Benzochloryl-related experiments and processes, aiding in the extraction, purification, and synthesis of these compounds.
Pesticides such as Methoxychlor and Dieldrin are also structurally similar to Benzochloryl, highlighting the diverse range of applications for these types of halogenated organic molecules.
When working with Benzochloryl and related compounds, techniques like using Nitrocellulose membranes can be helpful for separation, purification, and analysis purposes.
PubCompare.ai's AI-driven platform can streamline your Benzochloryl research by helping you locate relevant protocols from literature, pre-prints, and patents, and providing AI-driven comparisons to identify the best protocols and products.
This can enhance the reproducibility and reliability of your findings, optimizing your research process.