Ethyl Methanesulfonate
It is commonly used as a mutagen in genetic research and as an alkylating agent in organic synthesis.
This versatile reagent can induce a variety of genetic alterations, making it a valuable tool for studying mutagenesis and gene function.
PubCompare.ai's AI-driven platform can help locate the best protocols from literature, preprints, and pateents, using intelligent comparisons to identify the most effective methods for working with Ethyl Methanesulfonate.
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Most cited protocols related to «Ethyl Methanesulfonate»
Arabidopsis thaliana (L.) Heynh. var. Columbia (Col-0) and var. Landsberg (erecta mutant) (Ler) were used as wild-type strains. The sub-1 mutant was described previously [25] (link). Plants were grown in a greenhouse under Philips SON-T Plus 400 Watt fluorescent bulbs on a long day cycle (16 hrs light). Dry seeds were sown on soil (Patzer Einheitserde, extra-gesiebt, Typ T, Patzer GmbH & Co. KG, Sinntal-Jossa, Germany) overlying perlite, stratified for 4 days at 4°C and then placed in the greenhouse. Plant trays were covered for 7–8 days to increase humidity and support equal germination. Ler seeds mutagenized with ethylmethane sulfonate (EMS) were obtained from Lehle Seeds (Round Rock, TX, USA). 60'000 M2 plants, corresponding to about 7'500 M1 plants, were screened for plants exhibiting a sub-like phenotype. All sub-like mutants described in this paper were outcrossed three times to Ler prior to further analysis. Two qky T-DNA insertion mutants (line SALK_140123 and SALK_043901) [112] (link) were obtained from the ABRC (
A germ-line tranformant expressing an myc epitope-tagged wild-type β spectrin transgene on chromosome 2 was produced using a previously described strategy (
Mutations in the Drosophila β spectrin gene were produced by chemical mutagenesis. The screen was based on recovery of X-linked lethal mutations by complementation with Dp(1;3)BS3iD3, a duplication of the 16A-F region of the X chromosome on chromosome 3. Males from an isogenized Oregon R stock were fed 24.5 mM ethyl methane sulfonate using standard methods (
Balanced stocks of each mutant over a FM7[Kruppel-GFP] chromosome (
Mitotic clones in adult retina were generated using hs-FLP1; FRTneo82B, P{w+}96A (Xu and Rubin, 1993 (link)). Mitotic clones in larval eye discs were generated using ey-FLP; FRT82neo, GMR-myrGFP-3R (see below). To facilitate exogenous protein expression in larval Garland cells, UAS-derived transgenes (UAS-GFP–rab7 and UAS-GFP–Clc) were driven with Act5C-GAL4 lines.
UAS-myc::Hsc70-C1 and alleles of Hsc70-4 were obtained from Spyros Artavanis-Tsakonas (Massachusetts General Hospital/Harvard Medical School, Boston, MA). HRP-Boss flies were obtained from Helmut Kramer (University of Texas Southwestern, Dallas, TX). UAS-GFP–rab7 flies were obtained from Marcos A. González-Gaitán (Max-Planck Institute, Dresden, Germany). Act5C-GAL4 (No. 4414), Sev-GAL4 (No. 5793), and sca-GAL4109–68 (No. 6479) were obtained from the Bloomington Drosophila stock center (Bloomington, IN).
Most recents protocols related to «Ethyl Methanesulfonate»
Example 1
Since the biosynthetic pathway of anatabine and its associated genes is not completely known, a novel genetic variation was created in a population of tobacco plants to identify plants that have a significantly reduced ability to biosynthesize anatabine. These plants very likely have a mutated non-functional gene, critical for anatabine biosynthesis.
A population of the Flue-cured variety “401” was used in these experiments. Approximately 5000 seeds were treated with 0.6% ethyl methane sulfonate and germinated. M1 plants were grown in the field and M2 seeds were collected. Fifteen hundred M2 seeds were germinated and grown in 4-inch pots. At 50% flowering stage, plants were topped. Leaf samples were collected 2 weeks after topping and the samples screened for anatabine levels using high performance thin layer chromatography (HP-TLC) and gas chromatography.
After screening for alkaloids, two Flue Cured (FC) 401 ultra-low anatabine (ULA) lines were selected for trait development. It is noted that the amount of nicotine in both ULA lines is unchanged.
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More about "Ethyl Methanesulfonate"
This reagent is widely used in genetic research and organic synthesis due to its powerful mutagenic properties.
EMS is capable of inducing a variety of genetic alterations, making it a valuable tool for studying mutagenesis and gene function.
Researchers often utilize EMS in conjunction with other compounds, such as MS-222 (Tricaine methanesulfonate), DMSO, and Collagenase type I, to achieve desired outcomes in their experiments.
MS-222, for example, is a common anesthetic used in aquatic research, while DMSO is a popular solvent that can enhance the penetration of EMS into cells.
The effects of EMS can be wide-ranging, from inducing point mutations to larger-scale chromosomal changes.
Ethyl 3-aminobenzoate methanesulfonate salt, also known as Ethyl methanesulfonate (EMS), is a related compound that shares some of EMS's properties and applications.
PubCompare.ai's AI-driven platform can help streamline your research on EMS by locating the best protocols from literature, preprints, and patents.
The platform's intelligent comparisons can identify the most effective methods for working with this powerful mutagen, allowing you to optimize your research and find the optimal solution.
Whether you're studying mutagenesis, gene function, or utilizing EMS in organic synthesis, PubCompare.ai can be a valuable resource in your research endeavors.
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