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Paraquat

Paraquat is a highly toxic, non-selective herbicide used in agriculture to control a wide variety of weeds.
Exposure to paraquat can lead to severe respiratory distress, organ damage, and even death.
Researchers utilize paraquat to study the mechanisms of oxidative stress and its role in Parkinson's disease and other neurodegenerative disorders.
PubCompare.ai offers an intuitive platform to optimize paraquat research, locating the best protocols from literature, preprints, and patents using AI-driven comparisons.
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Most cited protocols related to «Paraquat»

To study the effect of different oxidative stressors on reporter gene expression in transgenic flies, 1 week old flies that were mated for one day and then separated into males and females, were exposed to different oxidative stressors. The animals were starved for 2 hours and then fed a solution of 5% sucrose ±20 mM Paraquat (Sigma-Aldrich, St. Louis, MO). To examine the effect of oltipraz on reporter flies, similarly collected reporter flies were fed food supplemented with 1 mM oltipraz (LKT Laboratories Inc., St. Paul, MN) for 48 hours. 15–20 flies were used in each group for these experiments and 3–5 representative flies were chosen randomly for imaging. Similar results were seen in multiple experiments. Western blot for reporter proteins on pools of flies also confirmed the induction of reporter transgenes with different treatments (data not shown).
To assess the effect of different oxidative stressors and chemicals on the cell-based reporters S2 cells were transiently transfected with the reporter plasmids by the calcium phosphate method. 8 hours after the PBS wash and medium change, the transfected cells were transferred to 96-well plates and treated with 25 µM oltipraz, 100 µM DEM, 100 µM NaAsO2 (J.T.Baker (Phillipsburg, NJ)) and were incubated at 25°C for 24 hrs.
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Publication 2012
Animals Animals, Transgenic Calcium Phosphates Cells Diptera Females Food Gene Expression Males oltipraz Paraquat Plasmids Proteins Sucrose Transgenes Vision Western Blot
In vitro maturation of hydrogenases was achieved by incubating 800 ng apo-protein (0.04 μM HYDA1) under strictly anaerobic conditions in 400 μl of 0.1 M potassium phosphate buffer, pH 6.8, with 2 mM sodium dithionite (NaDT) at 25°C for 30 min with a 10-fold molar excess of [2Fe]MIM if not stated otherwise. Subsequent in vitro activity measurements using NaDT-reduced methyl viologen as artificial electron donor were done as previously described33 . Maturation of HYDA1MIM for EPR and FTIR measurements was carried out with 150 μM apoHYDA1. The protein was subsequently purified and re-buffered to 0.01 M Tris-HCl, pH 8.0, 2 mM NaDT, by size exclusion chromatography using a NAP™ 5 column (GE Healthcare) and concentrated to 500 μM for EPR and FTIR measurements using Amicon Ultra centrifugal filters 10K (Millipore). Spectra of 12 mM [2Fe]MIM were recorded in 20 mM HEPES buffer pH 7.5, 100 mM KCl.
Publication 2013
Buffers Electrons Gel Chromatography HEPES Hydrogenase Molar Paraquat potassium phosphate Proteins Sodium Dithionite Spectroscopy, Fourier Transform Infrared Tissue Donors
One-week-old young seedlings aseptically grown on 1/2 MS agar medium were transferred into pots filled with the same amount of compost soil and grown for another 1 or 2 weeks before stress treatments were applied. For drought tolerance assay, soil-grown plants were fully watered, and then withheld irrigation for 4 weeks, followed by rewatering plants. Survival rates were scored 1 week after rewatering. For salt tolerance assay, soil-grown plants were treated with progressively applied high salt stress by irrigating plants with NaCl solutions of stepwisely increasing concentrations (50, 100, and 200 mM) every 4 days and lasting at the concentration of 200 mM for 12 days when chlorophyll contents were measured according to the method (Lichtenthaler 1987 (link)). Cold treatment was performed by transferring the plants into a low temperature incubator for desired durations (−4°C, Sanyo, MIR-253). After 7 days-recovery under normal growth conditions, the survival rates were recorded. For oxidative stress treatment, leaves of similar developmental stages (7th and 8th rosette leaves) were detached from 3 or 4 week-old plants aseptically grown on 1/2 MS agar medium and floated abaxial side up in 2 μM methyl viologen (MV, Sigma–Adrich) solution under controlled growth conditions. Chlorophyll contents of the detached leaves were measured as described (Lichtenthaler 1987 (link)).
Publication 2011
Agar Biological Assay Chlorophyll Cold Temperature Drought Tolerance Growth Disorders Marijuana Abuse MS 1-2 Oxidative Stress Paraquat Plants Salt Stress Salt Tolerance Seedlings Sodium Chloride
HeLa cells stably expressing YFP-Parkin using the Flp-In system (Invitrogen) were creating according to the manufacturer's instructions and maintained in 300 μg/ml hygromycin (Sigma-Aldrich). Rat cortical neurons were isolated on embryonic day 18 and grown in neurobasal media supplemented with B-27, l-glutamine, and penicillin/streptomycin. All cell culture materials were obtained from Invitrogen and all chemicals were obtained from Sigma-Aldrich. Chemicals were prepared from DMSO stock solutions, except paraquat, N-acetyl-cysteine, and 3-methyladenine, which were added fresh to media. Mfn1−/−,Mfn2−/− and Mfn1−/−,Mfn2−/− double knockout MEFs were generously donated by D.C. Chan (California Institute of Technology, Pasadena, CA), ATG5−/− MEFs were donated by N. Mizushima (Tokyo Medical and Dental University, Tokyo, Japan), Flp-In HeLa cells were donated by V.V. Lobanenkov (National Institutes of Health, Rockville, MD), and HeLa cells stably expressing GFP-LC3 were donated by A. Tolkovsky (Cambridge University, Cambridge, UK).
Publication 2008
3-methyladenine Acetylcysteine Cell Culture Techniques Cortex, Cerebral Dental Health Services Embryo Glutamine HeLa Cells hygromycin A mitofusin 2 protein, human Neurons Paraquat PARK2 protein, human Penicillins Streptomycin Sulfoxide, Dimethyl
Animals were grown in 96-well microtiter plates to allow for automated liquid handling to speed assay set up and phenotyping. In each well, the final volume was 50 µL of S medium solution and HB101 bacterial food. HB101 was prepared in large batches (greater than 20 L) to reduce assay-to-assay variability. The bacteria were prepared from cultures grown for 24 hr in Superbroth and then centrifuged to concentrate. HB101 bacteria were resuspended at 20% volume per volume in S medium and frozen at −80° (without glycerol) in 1-mL aliquots. Bacteria were thawed and fed to animals at a final concentration of 2% volume per volume. This amount of food was sufficient to sustain the number of offspring derived from a single fourth larval stage hermaphrodite with food left over at the end of the assay. For any mapping or causality experiment, we used the same HB101 culture to reduce the substantial variation that arises from bacteria grown in independent preparations. Solutions of S medium and bacterial food were prepared and then split for control and paraquat conditions.
After assay plates were prepared, one mid to late fourth larval stage animal was singled using a platinum wire to each 50-µL well. In the plate set up, we separated different genotypes with a wash well containing only S medium to decrease carry-over and mixing of independent genotypes from one well to the next. After all wells were populated with animals, the microtiter plate was sealed with a Breath-Easy film (USA Scientific) and placed in a humidity chamber lined with damp towels, closed, and then sealed with parafilm. We observed less than 5% of the well volume evaporated after four days under these conditions. Humidity chambers were placed into incubators set to 20° and shaking at 180 rpm. These conditions ensured that cultures and bacterial food was constantly mixing so animals would never enter hypoxia nor encounter regions of depleted food in the well. Animals were grown for 96 hr in these conditions and then prepared for measurement on the COPAS BIOSORT (Union Biometrica).
Two minutes before the animals were loaded on the COPAS BIOSORT, 200 µL of M9 with 50 mM sodium azide was added to the wells. The sodium azide kills and straightens the animals to ensure proper measurement of body length as the paralyzed animal passes through the flow cell. The COPAS BIOSORT sheath flow rate was kept constant at 9.8 mL per minute to decrease variability in length measurements as much as possible. Then, 96-well microtiter plates were aspirated using the ReFLx module with the BISORT set to “no-bubble-trap” mode. When the system is run in “no-bubble-trap” mode, all wells from a single microtiter plate can be measured in approximately 25 min and well-to-well contamination is less than 1%, which is further mitigated by the use of wash wells between unique genotypes. Extinction and time of flight minimums were 50 and 20, respectively. Green, yellow, and red photomultiplier tubes were set to 700, 700, and 900, respectively. Signal multipliers were set to 1.0 and signal gains set to 3.0. The COPAS BIOSORT software had Profiler II enabled. Flat comma-separated value files were analyzed using custom scripts in the R statistical computing environment. The COPAS BIOSORT software can not differentiate bubbles from animals as objects pass through the flow cell. For this reason, we trained a support vector machine to differentiate these two types of objects with 99.97% accuracy (Supporting Information, Figure S1). The support vector machine primarily uses optical density raw values to generate a binary linear classifier separating bubbles from animals. The raw data were read in, processed, and plotted using the COPASutils R package (Shimko and Andersen 2014 (link)). All of the raw and processed data (File S2) can be accessed on the Andersen lab GitHub page. High-throughput assay plates with paraquat were prepared at 1.5 mM paraquat (Chem Service, Inc.) or no paraquat from the same dilution of HB101 and S medium.
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Publication 2015
Alarmins Animals Bacteria Biological Assay Cells CHOP protocol Extinction, Psychological Food Freezing Genotype Glycerin Hermaphroditism High-Throughput Screening Humidity Hypoxia Larva Measure, Body Paraquat Platinum Sodium Azide Technique, Dilution Vision

Most recents protocols related to «Paraquat»

The E. coli strains were incubated in M9 medium supplied with 2 mM MgSO4, 0.1 mM CaCl2, 10 mM sodium nitrate, 0.01 mM NiCl2, 0.01 mM FeCl2, 50 μg/mL thiamine, and 20 μg/mL chloramphenicol under anaerobic conditions. To obtain anaerobic conditions, H2 and CO2 were used to purge the headspace of a bottle sealed with rubber. Approximately 4 × 109 bacteria cells were collected by centrifugation (10,000× g, at 4 °C) from 750 mL medium and sonicated in Tris buffer (100 mM Tris-HCl at pH 8.4, 3 mM dithioerythritol) on ice in an anaerobic chamber (Coy Laboratory Products, Inc., Grass Lake, MI, USA) filled with 9% H2, 15% CO2, and 76% N2 gas. The crude extracted proteins were collected from the supernatant by centrifugation (10,000× g, at 4 °C). The 2-oxoglutarate:ferredoxin oxidoreductase activity assays were performed using the method described in a previous report with modifications [15 (link)] in an anaerobic chamber (Coy Laboratory Products, Inc., Grass Lake, MI, USA). The activity of 2-oxoglutarate:ferredoxin oxidoreductase was determined by the reduction in succinyl-CoA (reduced methyl viologen:succinyl-CoA oxidoreductase). The assay was performed in a 1 mL volume containing 100 mM Tris-HCl at pH 8.4, 2 mM MgCl2, 4 mM methyl viologen, and 1 mM succinyl-CoA. Dithionite was added from 1 M stock solutions until the methyl viologen-containing assay solutions presented a faint blue color. The optical density changes were measured at 578 nm (methyl viologen, ε578 = 9.8 × 103 M–1 cm–1) after addition of the succinyl-CoA solutions. The optical densities were measured with a GeneQuant 1300 (GE Healthcare, Little Chalfont, Buckinghamshire, UK) in the anaerobic chamber at 35 °C. The result is displayed in Table 2.
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Publication 2023
alpha-Ketoglutarate Bacteria Biological Assay Cells Centrifugation Chloramphenicol Dithioerythritol Dithionite Escherichia coli Ferredoxin Magnesium Chloride Oxidoreductase Paraquat Poaceae Proteins Rubber sodium nitrate Strains succinyl-coenzyme A Sulfate, Magnesium Syncope Thiamine Tromethamine Vision
After 3 days of treatment, nematodes were transferred onto NGM plates supplied with 10 mM H2O2 or 10 mM paraquat, and each plate with 80 worms. The number of survival nematodes was counted per 30 min intervals (the H2O2 plates) and per 12 h intervals (the paraquat plates) until all the nematodes were dead [47 (link)]. The survival rate was calculated.
After 3 days of treatment, nematodes were transferred onto the fresh NGM plates (80 worms on each plate) and incubated at 35 °C for 10 h [48 (link)]. Numbers of survival nematodes were counted after the heat shock and the survival rate was calculated.
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Publication 2023
Aftercare Heat-Shock Response Helminths Nematoda Paraquat Peroxide, Hydrogen
The A. platensis powder was purchased from a local supplier in Shanghai. Strains used in this study were isolated from the sediment of the South China Sea and stored in the lab: Pseudoalteromonas sp. JS4-1 (GenBank accession number: MT116988), Pseudoalteromonas sp. B27-3, Pseudoalteromonas sp. B47-6, Pseudoalteromonas sp. B62-3, Pseudoalteromonas sp. WH05-1, Pseudoalteromonas sp. WH06-2, and Pseudoalteromonas sp. ZB23-2. Immortal human umbilical vein endothelial cell line (HUVECs) and human immortal keratinocyte line (HaCaT) were obtained from the National Collection of Authenticated Cell Cultures (Shanghai, China). Wild type C. elegans (N2) were obtained from Caenorhabditis Genetics Center (CGC).
Ninhydrin, penicillin-streptomycin solution (cell culture grade), trypsin-EDTA solution, dimethyl sulfoxide (DMSO), thiazolyl blue tetrazolium bromide (MTT), and phosphate buffered saline (PBS) were purchased from Sangon Biotech (Shanghai, China). Trisodium citrate dihydrate, SnCl2, n-propanol, FeSO4, 30% H2O2, cholesterol and glucose were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). In addition, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 1,10-phenanthroline monohydrate (OP), fluorescein sodium salt, 2,20-azobis (2-amidinopropane) dihydrochloride (AAPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), L-leucine, antifade mounting medium and NaN3 were purchased from Sigma-Aldrich, Ltd. (St. Louis, MO, USA). AmiconTM Ultra-15 centrifugal filter units were purchased from Millipore® (Billerica, MA, USA). Sephadex LH-20 was purchased from GE Healthcare Life Sciences (Uppsala, Sweden). Fetal bovine serum (FBS) was purchased from PAN-BiotechGibco company (Aidenbach, Germany). RPMI-1640 medium was purchased from Gibco® ThermoFisher Scientific Company (Waltham, MA, USA). Methyl viologen dichloride was purchased from Aladdin® (Shanghai, China). BCA assay kit, cell lysis buffer, ROS assay kit, catalase (CAT) assay kit, total superoxide dismutase (SOD) assay kit with NBT, cellular glutathione peroxidase (GSH-Px) assay kit with NADPH and lipid peroxidation MDA assay kit were purchased from Beyotime Biotechnology (Shanghai, China).
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Publication 2023
1-Propanol 2,2'-azobis(2-amidinopropane) Biological Assay Buffers Caenorhabditis Carboxylic Acids Catalase Cell Culture Techniques Cell Lines Cells Cholesterol diphenyl Edetic Acid Endothelium Fetal Bovine Serum Glucose Glutathione Homo sapiens Keratinocyte Leucine Lipid Peroxidation NADP NADPH peroxidase Ninhydrin Paraquat Penicillins Peroxidase, Glutathione Peroxide, Hydrogen Phenanthrolines Phosphates Powder Pseudoalteromonas Saline Solution sephadex LH 20 Sodium Azide Sodium Citrate Dihydrate Sodium Fluorescein Strains Streptomycin Sulfoxide, Dimethyl Superoxide Dismutase thiazolyl blue tetrazolium bromide Trolox C Trypsin Umbilical Vein
(±)-α-tocopherol; methyl viologen dichloride hydrate; DMEM:F12 medium; penicillin–streptomycin solution hybri-max (P/S); fetal bovine serum (FBS); L-glutamine (L-Glut); non-essential amino acid solution (NEAA); trypan blue; AM281; WIN55,212-2; mesylate salt; 2-mercaptobenzothiazole; and 1,5-diaminophtalene were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Methanol and acetonitrile were purchased from Panreac (Barcelona, Spain) and cesium chloride was purchased from HoneyWell (Charlotte, NC, USA).
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Publication 2023
2-mercaptobenzothiazole acetonitrile alpha-Tocopherol Amino Acids, Essential cesium chloride Fetal Bovine Serum Glutamine Mesylates Methanol Paraquat Penicillins Sodium Chloride Streptomycin Trypan Blue
Arabidopsis plants were grown for 28 days in control conditions, and then were treated with paraquat (15 μM, final concentration) for 1 h under control conditions or HL. Light stress was caused by continuous illumination of cells with the LSM 510 META Ar laser (excitation at 488 nm) as described [24 (link)]. The effective power was 30 mW. The laser intensity was increased by 1.5 times compared to the standard intensity.
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Publication 2023
Arabidopsis Cells Hartnup Disease Light Lighting Paraquat Plants

Top products related to «Paraquat»

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Paraquat is a chemical compound used as a laboratory reagent. It is a highly effective herbicide and desiccant. Paraquat is commonly used in research and analytical applications that require a potent oxidizing agent.
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Methyl viologen is a chemical compound used as a redox indicator and electron acceptor in various laboratory applications. Its core function is to facilitate the measurement and monitoring of oxidation-reduction reactions in experimental settings.
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Methyl viologen dichloride hydrate is a chemical compound commonly used in laboratory settings. It is a synthetic organic salt that functions as an electron acceptor. The core purpose of this product is to facilitate redox reactions and act as a mediator in various experimental procedures.
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Rotenone is a naturally occurring insecticide and piscicide derived from the roots of certain tropical plants. It is commonly used as a research tool in laboratory settings to study cellular processes and mitochondrial function. Rotenone acts by inhibiting the electron transport chain in mitochondria, leading to the disruption of cellular respiration and energy production.
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Paraquat (PQ) is a highly effective and versatile herbicide produced by Merck Group. It is a broad-spectrum contact herbicide used to control a wide range of annual and perennial weeds, grasses, and broadleaf plants. Paraquat disrupts photosynthesis within plant cells, leading to the rapid desiccation and death of the target vegetation.
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Paraquat dichloride hydrate is a chemical compound primarily used as a laboratory reagent. It functions as an electron acceptor in various chemical reactions and processes. No further details or interpretations are provided.
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
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Paraquat dichloride is a chemical compound used as a herbicide and desiccant in various agricultural applications. It is a synthetic organic compound that belongs to the bipyridylium class of chemicals. The core function of paraquat dichloride is to act as a contact herbicide, which means it kills plants upon direct application to the foliage.
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Paraquat is an analytical instrument used for the detection and quantification of the chemical compound paraquat in various samples. It is designed to provide accurate and reliable results for research, environmental monitoring, and other applications that require the analysis of paraquat.
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Hydrogen peroxide is a clear, colorless liquid chemical compound with the formula H2O2. It is a common laboratory reagent used for its oxidizing properties.

More about "Paraquat"

Paraquat, also known as methyl viologen or paraquat dichloride, is a highly toxic, non-selective herbicide commonly used in agriculture to control a wide range of weeds.
Exposure to paraquat can lead to severe respiratory distress, organ damage, and even death.
Researchers often utilize paraquat in studies to investigate the mechanisms of oxidative stress and its role in Parkinson's disease and other neurodegenerative disorders.
PubCompare.ai offers an intuitive platform to optimize paraquat research by locating the best protocols from scientific literature, preprints, and patents using AI-driven comparisons.
This powerful tool can help researchers achieve reproducible and seamless protocol comparisons, as well as product recommendations for their paraquat-based experiments.
In addition to paraquat, researchers may also explore the use of related compounds like methyl viologen dichloride hydrate, rotenone, and DMSO to study oxidative stress and its impact on various health conditions.
By leveraging the insights and capabilities of AI-enhanced research platforms like PubCompare.ai, scientists can streamline their paraquat-related investigations and make meaningful advancements in the understanding of Parkinson's disease and other neurodegenerative disorders.