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> Chemicals & Drugs > Hazardous or Poisonous Substance > Tretinoin

Tretinoin

Tretinoin, also known as all-trans-retinoic acid, is a naturally occurring retinoid and the carboxylic acid form of vitamin A.
It is widely used in the treatment of acne, photo-aged skin, and certain types of cancer.
Tretinoin works by regulating gene expression, cell differentiation, and cell proliferation.
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Most cited protocols related to «Tretinoin»

1
Cerebral Organoid Generation from Human Pluripotent Stem Cells
Human H9 ES (WA09) were obtained from WiCell at passage 26 with verified normal karyotype and contamination-free. iPS cells were obtained from System Biosciences (SC101A-1) verified pluripotent and contamination free. All human PSC lines were regularly checked and confirmed negative for mycoplasma. PSCs were maintained on CF-1 gamma irradiated MEFs (Global Stem) according to WiCell protocols. On day 0 of organoid culture, ESCs or iPSCs less than passage 50 were dissociated from MEFs by dispase treatment and MEFs were removed by gravity separation of stem cell colonies from MEFs before trypsinization of stem cells to generate single cells. 4500 cells were then plated in each well of an ultra-low binding 96-well plate (Corning) in hES media with low bFGF (5-fold reduced) and 50uM ROCK inhibitor49 (link) (Calbiochem).
EBs were fed every other day for 6 days then transferred to low adhesion 24-well plates (Corning) in neural induction media containing DMEM/F12, 1:100 N2 supplement (Invitrogen), Glutamax (Invitrogen), MEM-NEAA, and 1ug/ml Heparin50 (link) (Sigma). These began forming neuroepithelial tissues, which were fed every other day for 5 days. On Day 11 of the protocol, tissues were transferred to droplets of Matrigel (BD Biosciences) by pipetting into cold Matrigel on a sheet of Parafilm with small 3mm dimples. These droplets were allowed to gel at 37C and were subsequently removed from the Parafilm and grown in differentiation media containing a 1:1 mixture of DMEM/F12 and Neurobasal containing 1:200 N2 supplement (Invitrogen), 1:100 B27 supplement without vitamin A (Invitrogen), 3.5ul/L 2-mercaptoethanol, 1:4000 insulin (Sigma), 1:100 Glutamax (Invitrogen), 1:200 MEM-NEAA.
After 4 days of stationary growth, the tissue droplets were transferred to a spinning bioreactor containing differentiation media as above except B27 supplement with vitamin A (Invitrogen) was used. Since retinoic acid has been shown to be important for neuronal differentiation in vivo52 (link), we included it in the final media used to differentiate the cerebral organoids.
Lancaster M.A., Renner M., Martin C.A., Wenzel D., Bicknell L.S., Hurles M.E., Homfray T., Penninger J.M., Jackson A.P, & Knoblich J.A. (2013). Cerebral organoids model human brain development and microcephaly. Nature, 501(7467), 10.1038/nature12517.
Publication 2013
2-Mercaptoethanol Bioreactors Cells Cell Separation Common Cold Dietary Supplements dispase Enhanced S-Cone Syndrome Gamma Rays Gravity Homo sapiens Induced Pluripotent Stem Cells Insulin Karyotyping matrigel Mycoplasma Nervousness Neurons Organoids Pancreatic Stellate Cells Stem, Plant Stem Cells Tissues Tretinoin Vitamin A
2
Dopaminergic and Motoneuron Patterning
Dopaminergic patterning was initiated using BDNF, ascorbic acid, sonic hedgehog, and FGF8 in N2 media as previously reported22 (link), and maturation was performed in the presence of BDNF, ascorbic acid, GDNF, TGFb-1, and cyclic-AMP. Motoneuron patterning was performed using BDNF, ascorbic acid, sonic hedgehog, and retinoic acid in N2 media as previously reported18 (link).
Chambers S.M., Fasano C.A., Papapetrou E.P., Tomishima M., Sadelain M, & Studer L. (2009). Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nature biotechnology, 27(3), 275-280.
Publication 2009
Ascorbic Acid Cyclic AMP Erinaceidae FGF8 protein, human GDNF protein, human Hydrochloride, Dopamine Motor Neurons Tretinoin
3
Culturing Neural Progenitor Cells
The surface of the culture dishes (polystyrene Petri dishes and culture flasks, or glass coverslips in multiwell plates; Nunc) was first coated with 10 µg/ml Poly-Lysine (Sigma) overnight at room temperature. After several rinses in water, the surfaces were then coated with 5 µg/ml Laminin (Tebu-bio&Roche) at 37°C overnight. The plates or coverslips were stored at −20°C.
After removing excess coating solution, cells were plated directly onto the surface. The cultures were maintained in proliferation medium consisting of Neurobasal (Invitrogen), B27 or N2 supplement (Invitrogen), 2 mM Glutamax (Invitrogen), Pen-Strep (Sigma), 20 ng/ml human Fibroblast Growth Factor-2 (FGF2), and 20 ng/ml human Epidermal Growth Factor (EGF) (both from R&D or Pepro Tech). The medium was replaced with fresh medium the next day. Subsequently, the cultures were fed with new medium every two or three days by replacing 75% of the medium. We maintained a cell density of 104 cells/cm2. To induce and maintain differentiation the growth medium was replaced with either growth medium free of mitogens FGF2 and EGF, with or without 0.5 µM retinoic acid (Sigma), or with 0.5% fetal bovine serum and human Brain Derived Neurotrophic Factor (BDNF; 100 ng/ml). Recombinant human Insulin (Sigma) was used at a concentration of 20 µg/ml.
Babu H., Cheung G., Kettenmann H., Palmer T.D, & Kempermann G. (2007). Enriched Monolayer Precursor Cell Cultures from Micro-Dissected Adult Mouse Dentate Gyrus Yield Functional Granule Cell-Like Neurons. PLoS ONE, 2(4), e388.
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Publication 2007
Brain Cell Culture Techniques Cells Culture Media Dietary Supplements Epidermal growth factor Fetal Bovine Serum Fibroblast Growth Factor 2 Homo sapiens Hyperostosis, Diffuse Idiopathic Skeletal Insulin laminin-5 Lysine Mitogens Nerve Growth Factors Poly A Polystyrenes Streptococcal Infections Tretinoin
4
Directed Differentiation of Lung Progenitors from Pluripotent Stem Cells

Protocol full text hidden due to copyright restrictions

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Publication 2017
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide Ascorbic Acid Bone Morphogenetic Protein 4 Cells Chir 99021 Dietary Supplements dorsomorphin Endoderm Endometriosis Flow Cytometry Growth Factor Homo sapiens Lung matrigel NKX2-1 protein, human Proteins Protoplasm Serum Serum Albumin, Bovine Stem Cells Surrogate Markers thioglycerol Tretinoin Y 27632
5
Multicenter AML Clinical Trials Protocol
We obtained samples from patients participating in three prospective multicenter clinical trials of the German–Austrian AML Study Group: AML-HD98A, AML-HD98B, and AMLSG-07-04 (see the Supplementary Appendix, available with the full text of this article at NEJM.org).9 (link)–11 (link) In AML-HD98A, patients 18 to 65 years of age received induction chemotherapy consisting of idarubicin, cytarabine, and etoposide (ICE); high-risk patients were offered allogeneic stem-cell transplantation, intermediate-risk patients a stem-cell allograft (if a matched related donor was available) or intensive chemotherapy, and low-risk patients intensive chemotherapy. AMLSG-07-04 had a similar design, but patients 18 to 61 years of age were randomly assigned to receive ICE or ICE plus all-trans retinoic acid (ATRA) as induction therapy. In AML-HD98B, patients 58 to 84 years of age were randomly assigned to induction therapy with ICE or ICE plus ATRA, with further therapy dictated by the response (see Figs. S1 and S2 in the Supplementary Appendix). The median follow-up period was 5.9 years (Table S1 in the Supplementary Appendix). All patients provided written informed consent.
The last two authors guided the analysis and data interpretation.
Papaemmanuil E., Gerstung M., Bullinger L., Gaidzik V.I., Paschka P., Roberts N.D., Potter N.E., Heuser M., Thol F., Bolli N., Gundem G., Van Loo P., Martincorena I., Ganly P., Mudie L., McLaren S., O’Meara S., Raine K., Jones D.R., Teague J.W., Butler A.P., Greaves M.F., Ganser A., Döhner K., Schlenk R.F., Döhner H, & Campbell P.J. (2016). Genomic Classification and Prognosis in Acute Myeloid Leukemia. The New England journal of medicine, 374(23), 2209-2221.
Publication 2016
Cytarabine Etoposide Figs Idarubicin Induction Chemotherapy Neoadjuvant Therapy Pharmacotherapy Stem Cells Therapeutics Tissue Donors Transplantation, Homologous Transplantations, Stem Cell Tretinoin

Most recents protocols related to «Tretinoin»

1
Tretinoin Gel Formulation and Preparation
Not available on PMC !

Example 2

A composition comprising Tretinoin as an active ingredient:

IngredientsConcentration (w/w %)
Oleic acid4.00
Isopropanol6.00
BHT (Butylated Hydroxytoluene)0.02
Sorbic acid0.10
Tretinoin0.10
Silica microspheres0.70
Natrosol (HEC)1.50
Xanthan gum0.80
Trolamine1.20
Benzyl alcohol0.80
Glycerin15.00
Waterq.s. 100%

The process for the preparation of the composition was as follows:

    • 1. Trolamine, Natrosol (HEC) and xanthan gum were added gradually to the water while stirring at high speed using mixer propeller;
    • 2. The mixture of oleic acid, isopropanol, BHT, sorbic acid and tretinoin was heated to 50° C. while stirring then cooled to the room temperature;
    • 3. Silica microspheres were added to the stage 2 and the resultant mixture was stirred for at least one hour;
    • 4. Benzyl alcohol and Glycerin were added to stage 1
    • 5. Stage 4 was added to the mixer reactor and stirred vigorously.

An opaque yellowish gel was obtained.

US11865208B2. Pharmaceutical compositions comprising silica microspheres (2024-01-09). SOL-GEL TECHNOLOGIES LTD. [IL]. Inventors: Marina Shevachman [IL], Amira Ze' Evi [IL], Eilon Asculai [IL], Batella Benyaminovich [IL], Nir Avram [IL], Chaim Aschkenasy [IL].
Full text: Click here
Patent 2024
Benzyl Alcohol Glycerin Isopropyl Alcohol Microspheres Oleic Acid Pharmaceutical Preparations Silicon Dioxide Sorbic Acid Tretinoin triethanolamine xanthan gum
2
Tretinoin-Based Topical Gel Formulation
Not available on PMC !

Example 4

A composition comprising Tretinoin as active ingredient:

IngredientConcentration (w/w %)
Oleic acid5.00
Isopropanol10.00
BHT (Butylated Hydroxytoluene)0.02
Sorbic acid0.10
Tretinoin0.10
Silica microspheres0.70
CMC Na (carboxymethyl cellulose sodium)2.40
Natrosol (HBC)0.50
Glycerin5.00
Benzyl alcohol0.80
Poloxamer 4070.20
P. Waterq.s. 100%

The process for the preparation of the composition was as follows:

    • 1. CMC Na (carboxymethyl cellulose sodium) and Natrosol (HEC) were dispersed in water until a clear gel was formed
    • 2. Glycerin and benzyl alcohol were added to stage 1 and mixed;
    • 3. Oleic acid, isopropanol, BHT, sorbic acid, Poloxamer 407 and tretinoin were heated to 50° C. while stirring until clear solution was obtained. Then the solution was cooled to the room temperature;
    • 4. Silica Microspheres were added to the cooled oily phase and resultant mixture was stirred for at least one hour;
    • 5. Stage 4 was added to the stage 2 and stirred for one hour under vacuum.

An opaque yellowish gel was obtained.

US11865208B2. Pharmaceutical compositions comprising silica microspheres (2024-01-09). SOL-GEL TECHNOLOGIES LTD. [IL]. Inventors: Marina Shevachman [IL], Amira Ze' Evi [IL], Eilon Asculai [IL], Batella Benyaminovich [IL], Nir Avram [IL], Chaim Aschkenasy [IL].
Full text: Click here
Patent 2024
Benzyl Alcohol Ethanol Glycerin Isopropyl Alcohol Microspheres Oils Oleic Acid Pharmaceutical Preparations Poloxamer 407 Silicon Dioxide Sodium Carboxymethylcellulose Sorbic Acid Tretinoin Vacuum
3
Expansion and Differentiation of Pluripotent Cells
Not available on PMC !

Example 5

Selected cells can be expanded/grown in Dulbecco's modified Minimal Essential Medium (DMEM) supplemented with glutamine, beta.-mercaptoethanol, 10% (by volume) horse serum, and human recombinant Leukemia Inhibitory Factor (LIF). LIF replaces the need for maintaining selected cells on feeder layers of cells, (which may also be employed) and is essential for maintaining selected cells in an undifferentiated, multipotent, or pluripotent state, such cells can be maintained in Dulbecco's modified Minimal Essential Medium (DMEM) supplemented with glutamine, beta.-mercaptoethanol, 10% (by volume) horse serum, and human recombinant Leukemia Inhibitory Factor (LIF). The LIF replaces the need for maintaining cells on feeder layers of cells, (which may also be employed) and is essential for maintaining cells in an undifferentiated state (per U.S. Pat. No. 6,432,711).

In order to initiate the differentiation of the selected cells into neuronal cells, the cells are trypsinized and washed free of LIF, and placed in DMEM supplemented with 10% fetal bovine serum (FBS). After resuspension in DMEM and 10% FBS, 1×106 cells are plated in 5 ml DMEM, 10% FBS, 0.5 microM retinoic acid in a 60 mm Fisher bacteriological grade Petri dishes, where the cells are expected to form small aggregates. Aggregation aids in proper cell differentiation. High efficiency transfection with (or overexpression of) appropriate neuronal transcription factors and small RNAs can occur before or after plating in DMEM, FBS, and retinoic acid. (See U.S. Pat. Nos. 6,432,711 and 5,453,357 for additional details).

US11859168B2. Electroporation, developmentally-activated cells, pluripotent-like cells, cell reprogramming and regenerative medicine (2024-01-02). None [US]. Inventors: Christopher B. Reid [US], Melissa Braga [US], Lilian N. Santamaria [US], Ashley N. Wickrema [US], Marinne D. Wickrema [US], Anthony Hao Dinh [US], Yaman Eksioglu [US], Mat Hoang Ho [US].
Full text: Click here
Patent 2024
2-Mercaptoethanol Acquired Immunodeficiency Syndrome Cells Differentiations, Cell Equus caballus Feeder Layer Cells Fetal Bovine Serum Glutamine Hyperostosis, Diffuse Idiopathic Skeletal LIF protein, human Neurons RNA Serum Transcription Factor Transfection Tretinoin
4
Tretinoin-Based Topical Gel Formulation
Not available on PMC !

Example 3

A composition comprising Tretinoin as active ingredient:

IngredientsConcentration (w/w %)
Oleic acid4.00
Isopropanol5.00
BHT (Butylated Hydroxytoluene)0.02
Sorbic acid0.10
Tretinoin0.10
Silica microspheres0.50-1.00
Poloxamer 407 A12.00-15.00
Poloxamer 407 B0.2-1.0
Benzyl alcohol0.80
Glycerin15.00 
Sodium hydroxide, dilutedAdjust pH
Waterq.s. 100%

The process for the preparation of the composition was as follows:

    • 1. Poloxamer 407 A was added to the water while stirring at high speed using mixer propeller;
    • 2. The mixture of oleic acid, isopropanol, BHT, sorbic acid, Poloxamer B and tretinoin was heated to 50° C. while stirring and then was cooled to the room temperature;
    • 3. Silica Microspheres were added to the stage 2 and resultant mixture was stirred for at least one hour;
    • 4. Benzyl alcohol and glycerin were added to stage 1 and stirred;
    • 5. Stage 3 was added to the mixer reactor and stirred vigorously;
    • 6. Sodium hydroxide was added for pH adjustment.

An opaque yellowish gel was obtained.

US11865208B2. Pharmaceutical compositions comprising silica microspheres (2024-01-09). SOL-GEL TECHNOLOGIES LTD. [IL]. Inventors: Marina Shevachman [IL], Amira Ze' Evi [IL], Eilon Asculai [IL], Batella Benyaminovich [IL], Nir Avram [IL], Chaim Aschkenasy [IL].
Full text: Click here
Patent 2024
Benzyl Alcohol Ethanol Glycerin Isopropyl Alcohol Microspheres Oleic Acid Pharmaceutical Preparations Poloxamer Poloxamer 407 Silicon Dioxide Sodium Hydroxide Sorbic Acid Tretinoin
5
Neuronal Differentiation of IMR-32 Cells
Neuronal differentiation of IMR-32 cells was conducted as previously described [1 (link)]. Briefly, cells were plated in 6 well dishes at a density of 50,000/well in normal growth media. After 24 hours of incubation, cells were treated with BMAA and/or all-trans retinoic acid (ATRA) (ACROS Organics, Geel, Belgium) at the indicated concentrations by addition to the media and returned to incubation. Cell morphology, analysis of dendrite formation using ImageJ, and RNA extraction for qPCR was conducted following 5 days of incubation post-treatment.
Burton B., Collins K., Brooks J., Marx K., Renner A., Wilcox K., Moore E., Osowski K., Riley J., Rowe J, & Pawlus M. (2023). The biotoxin BMAA promotes dysfunction via distinct mechanisms in neuroblastoma and glioblastoma cells. PLOS ONE, 18(3), e0278793.
Full text: Click here
Publication 2023
Cells Culture Media Dendrites Differentiations, Cell Hyperostosis, Diffuse Idiopathic Skeletal Neurons Tretinoin

Top products related to «Tretinoin»

1
Retinoic acid by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, China, Canada, Australia, Sao Tome and Principe, Israel, Japan, Netherlands
Retinoic acid is a chemical compound commonly used in laboratory settings. It is a form of vitamin A that plays a role in various biological processes. This product serves as a key reagent for researchers and scientists working in the fields of cell biology, developmental biology, and pharmacology.
2
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
3
Penicillin streptomycin by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
4
Dimethyl sulfoxide (dmso) by Merck Group
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
5
Dmem f12 by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Japan, Canada, Australia, Italy, Switzerland, Belgium, New Zealand, Spain, Denmark, Israel, Macao, Ireland, Netherlands, Austria, Hungary, Holy See (Vatican City State), Sweden, Brazil, Argentina, India, Poland, Morocco, Czechia
DMEM/F12 is a cell culture medium developed by Thermo Fisher Scientific. It is a balanced salt solution that provides nutrients and growth factors essential for the cultivation of a variety of cell types, including adherent and suspension cells. The medium is formulated to support the proliferation and maintenance of cells in vitro.
6
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
7
Glutamax by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, France, Switzerland, Canada, Australia, Japan, China, Belgium, Italy, Austria, Denmark, Spain, Netherlands, Sweden, Ireland, New Zealand, Israel, Gabon, India, Argentina, Macao, Poland, Finland, Hungary, Brazil, Slovenia, Sao Tome and Principe, Singapore, Holy See (Vatican City State)
GlutaMAX is a chemically defined, L-glutamine substitute for cell culture media. It is a stable source of L-glutamine that does not degrade over time like L-glutamine. GlutaMAX helps maintain consistent cell growth and performance in cell culture applications.
8
L glutamine by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, France, Canada, Switzerland, Italy, Australia, Belgium, China, Japan, Austria, Spain, Brazil, Israel, Sweden, Ireland, Netherlands, Gabon, Macao, New Zealand, Holy See (Vatican City State), Portugal, Poland, Argentina, Colombia, India, Denmark, Singapore, Panama, Finland, Cameroon
L-glutamine is an amino acid that is commonly used as a dietary supplement and in cell culture media. It serves as a source of nitrogen and supports cellular growth and metabolism.
9
Non essential amino acids (neaa) by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, Canada, France, Japan, China, Australia, Switzerland, Belgium, Italy, Austria, Sweden, Ireland, Spain, Gabon, Brazil, Netherlands, Holy See (Vatican City State), Israel, Argentina, Colombia, Sao Tome and Principe
Non-essential amino acids are a group of amino acids that can be synthesized by the human body and are not required to be obtained through diet. These amino acids play a fundamental role in various biological processes, including protein synthesis and cellular function.
10
Penicillin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Canada, Japan, Australia, Switzerland, Italy, Israel, Belgium, Austria, Spain, Brazil, Netherlands, Gabon, Denmark, Poland, Ireland, New Zealand, Sweden, Argentina, India, Macao, Uruguay, Portugal, Holy See (Vatican City State), Czechia, Singapore, Panama, Thailand, Moldova, Republic of, Finland, Morocco
Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.

More about "Tretinoin"

vitamin A, retinoic acid, FBS, penicillin, streptomycin, DMSO, DMEM/F12, DMEM, GlutaMAX, L-glutamine, non-essential amino acids