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House Dust

House Dust: A complex mixture of particulate matter, including fibers, microorganisms, and chemicals, commonly found in indoor environments.
Analyzing the composition and characteristics of house dust can provide valuable insights into indoor air quality, potential health risks, and environmental exposures.
Researchers leveraging PubCompare.ai can optimize their house dust studies by identifying the most accurate and reproducable methids from the literature, preprints and patents.
This AI-driven platform enables data-driven comparisons to enhance the success of house dust research projects.

Most cited protocols related to «House Dust»

The design of the Gulf STUDY is described in detail elsewhere (Kwok et al., in press ). In brief, persons who were involved in any aspect of DWH oil spill work and/or completed worker safety training in anticipation of performing spill-related work were identified from administrative records. Potential participants were recruited by telephone and invited to complete a 30–60 minute telephone enrollment interview. Participants were required to be at least 21 years of age at enrollment. Interviews were conducted in English, Spanish, or Vietnamese. Enrollment interviews were conducted from March 2011 to March 2013. Individuals who completed the telephone interview constitute the GuLF STUDY Cohort (N=32,608). All cohort members who lived in one of the Gulf States (Alabama, Florida, Louisiana, Mississippi, and east Texas) and spoke English or Spanish were invited to participate in a home visit. Home visits were conducted from May 2011 to May 2013. The Home Visit Subcohort is composed of the 11,193 cohort members who completed a home visit. Only 136 (1.2%) home visit participants were known to be still engaged in spill-related work at the time of their home visit, with a median of 21 months and maximum of 35 months since the end of spill-related work among all home visit participants. Finally, a stratified random sample of Home Visit Subcohort members residing in selected coastal and adjacent counties in Louisiana and Alabama and oversampled for oil spill workers constitute the Biomedical Surveillance Subcohort (N=4,050; 91% workers). This subcohort was established for characterization of blood parameters (in assays requiring fresh blood), for detailed urinalysis at the time of the home visit, and for possible invitation to periodic follow-up clinical examinations depending on proximity to subsequently established clinics in those two states.
Certified Medical Assistants (CMA), who have been trained in phlebotomy and other clinical procedures as part of their professional certification, served as field agents and underwent extensive additional training and certification by the GuLF STUDY research team prior to conducting the home visits. They were also subjected to regular quality control review and day-to-day oversight by the GuLF STUDY research team central field manager and a field manager located in each state.
During the home visit, field agents obtained informed consent, collected several physiologic and anthropometric measures, conducted a pulmonary function test, administered an additional questionnaire, and collected biological specimens and house dust samples. The three hour home visits were conducted 7 days per week, from early morning into the early evening. Whenever possible, home visits for members of the Biomedical Surveillance Subcohort were scheduled early on Monday through Thursday to facilitate overnight shipping of specimens. An average of 119 home visits per week was conducted.
Characteristics of the Home Visit Subcohort are shown in Table 1.
Publication 2017
Biological Assay Biopharmaceuticals BLOOD Hispanic or Latino House Dust Petroleum Pollution Phlebotomy Physical Examination physiology Safety Specimen Collection Tests, Pulmonary Function Urinalysis Vietnamese Workers
CCAAPS is an ongoing prospective birth cohort study. The study’s purpose is to determine whether infants who are exposed to DEP are at an increased risk for developing atopy and allergic respiratory diseases and to determine whether this effect is modified in a genetically at-risk population. The study methods, population, and sampling methodology are described in detail elsewhere (Hu et al. 2006 ; LeMasters et al. 2006 (link); Martuzevicius et al. 2004 ; Ryan et al. 2005 (link)). Briefly, eligible infants were identified from birth records, and the addresses obtained using these records were geocoded using EZLocate software (TeleAtlas Inc., Menlo Park, CA). Only those infants whose address was assigned the highest match code (TeleAtlas Inc. 2006 ) were included in this analysis. We computed the distance to the nearest road with > 1,000 trucks daily (“major road”) using the geoprocessing extension available for ArcView GIS 3.2 (Environmental Systems Research Institute Inc., Redlands, CA). Institutional review board approval was obtained before subject recruitment, and all enrolled parents gave written informed consent before their own and their infants’ study participation. Parents were recruited when infants were approximately 6 months of age and screened for allergy symptoms. Infants of atopic parents [confirmed with a skin prick test (SPT)] were enrolled. CCAAPS has continued prospectively evaluating enrolled infants with annual SPTs, physical examinations, questionnaires, and environmental exposure assessments, including exposure to house dust, mold, and environmental tobacco smoke (ETS) (Biagini et al. 2006 (link); Cho et al. 2006 (link); Osborne et al. 2006 (link)).
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Publication 2006
Environmental Exposure Ethics Committees, Research Fungus, Filamentous House Dust Hypersensitivity Infant Nicotiana tabacum Parent Physical Examination Population at Risk Respiration Disorders Respiratory Rate Smoke Test, Skin
Concentrations of tobacco alkaloids and tobacco-specific nitrosamines were determined using previously described analytical protocols7 (link), 8 (link), with a modified extraction procedure, described below and in the Supporting Information, Figure S1. Each dust sample was homogenized and fractionated using a mechanical shaker equipped with a 100-mesh sieve to obtain dust particles smaller than 150 μm. Fine dust samples were accurately weighed (66 ± 11 mg) into 16x125 mm culture tubes and to each sample was added 150 µL of aqueous internal standard solution (containing d4-nicotine, d4-NNN, d4-NNK, d3-NNA, d4-NAB, d4-NAT, d9-cotinine, d4-myosmine, d4-N-formylnornicotine, d8-nicotelline, and d4-2,3’-bipyridine; for details see Hang et al.8 (link)), 1.5 mL distilled water, and 0.5 mL of 1M sulfuric acid. The mixture was vortexted and 10 mL of 70:30 toluene:butanol was added. The tubes were then sonicated at 55°C for 1 hour with intermittent vortexing. The tubes were centrifuged, frozen in a dry ice-acetone bath and the toluene:butanol phase discarded. After the remaining aqueous phase was made basic with 1 mL of 45% potassium carbonate 5% tetrasodium EDTA, 10 mL of 45:45:10 dichloromethane:pentane:ethyl acetate was added. The samples were extracted using vortex mixing, centrifuged, and frozen again in a dry ice-acetone bath and the organic layer divided into two sets of 13x100 mm culture tubes for analysis by GC-MS (Agilent 6890N, for nicotine) and liquid chromatography-tandem mass spectrometry (Thermo Fisher Vantage LC-MS/MS, for all other analytes), as previously described.7 (link), 8 (link) Representative LC-MS/MS chromatograms are provided in the Supporting Information, Figures S2 and S3. Concentrations were calculated using the instrument data system software, aqueous standards spanning the measured concentration range, and calibration curves prepared from analyte/internal standard peak area ratios and analyte concentrations using linear regression with 1/X weighting. The precision of the analytical method was demonstrated using National Institute of Standards and Technology Standard Reference Material 2585 (Organic Contaminants in House Dust). Table S1 (Supporting Information) shows coefficients of variation for each analyte in nine analytical replicates of three extracts of the standard reference material ranging in sample mass from 31 to 110 mg.
Publication 2015
Acetone Alkaloids Bath Butanols Carbonates Cotinine Dry Ice Edetic Acid, Potassium Salt ethyl acetate Freezing Gas Chromatography-Mass Spectrometry House Dust Liquid Chromatography Methylene Chloride myosmine Nicotiana tabacum Nicotine Nitrosamines pentane Sulfuric Acids Tandem Mass Spectrometry Toluene
Settled surface dust was collected using the Omega HEPA Vacuum with a Dust Collection Filter Sock (Midwest Filtration Company, Cincinnati, OH) from 5 different locations within the month of September 2008: floor of the operating facility of a swine confinement facility (housing approximately 400-600 hogs), floor of the feeding barn of a dairy farm (approximately 50 in the building sampled, 2500 total on the facility), floor of the storage facility of a grain elevator (feed grain/feedmill), domestic home without pets (pets never allowed during home ownership), and domestic home with pet, dog (~60 pound dog). House dust was collected in the carpeted living room. In each facility three 1m2 areas were sampled vacuuming the whole area in two orthogonal passes. Dust samples in the Filter Sock were immediately placed in sterile Whirl-Pak bags (Nasco, Ft. Atkinson, WI). Samples were stored at −20°C.
For chemical marker analysis studies, dust samples (5 mg) were extracted with 10ml of 0.05% TWEEN-20 by vortexing for 1 min followed by 1 hr at 100 rpm on a rotary mixer at 20°C. The Tween extraction solution was then aliquoted for separate analysis of endotoxin (rFC), 3-OHFA (GC/MS/MS), muramic acid (GC/MS/MS), and ergosterol (GC/MS). Samples for mass spectrometry were not centrifuged before aliquoting. Individual aliquots were stored at −80°C, then lyophilized prior to sample digestion for mass spectrometry. Although Tween extraction is standard practice for mass spectrometry analysis, Tween is cellular toxic, and thus, not recommended for cell culture studies. All samples were analyzed in triplicate (i.e. three 5 mg extracts were used from each dust sample).
For cell culture studies, the dust samples were placed into solution and sterile filtered by a standard published procedure (Romberger et al. 2002 (link)). Briefly, 100 mg of each dust sample was placed in 1 ml of Hanks’ balanced salt solution (HBSS) without calcium (Biofluids). The mixture was vortexed for 1 min and allowed to stand at room temperature for 1 hour. The mixture was centrifuged at x g for 10 min, and the supernatant was recovered and centrifuged again. The final supernatant was filter (0.22 μm) sterilized. Individual aliquots were stored at −80°C prior to cell culture studies. HBSS extractions were also analyzed by rFC and mass spectrometry.
Publication 2010
Calcium Cell Culture Techniques Cells Cereals Digestion Endotoxins Ergosterol Filtration Gas Chromatography-Mass Spectrometry Hanks Balanced Salt Solution House Dust Mass Spectrometry Muramic Acid Pigs Sterility, Reproductive Strains Tandem Mass Spectrometry Tween 20 Tweens

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Publication 2018
Biological Assay Cells House Dust tris(1,3-dichloroisopropyl) phosphate tris(2-carboxyethyl)phosphine

Most recents protocols related to «House Dust»

Daily intake
(EDI) of plasticizers via dust ingestion or dermal contact was determined
using the following equations29 (link),45 (link) where EDI is the estimated daily intake (ng/kg body weight/day), C is the concentration of a chemical in house dust (ng/g),
IEF is the indoor exposure fraction (hours spent over a day in homes),
DIR is the dust ingestion rate (g/day), BW is body weight (kg), BSA
is body surface area (cm2/day), SAS is the amount of solid
particles adhered onto skin (mg/cm2), and FA is the fraction
of a chemical absorbed through the skin. We assumed a 100% absorption
of chemicals from ingested dust. Due to the lack of experimental and
model data of skin absorption of NPPs, the skin absorption fraction
of NPPs was assumed to be 0.000031 (low exposure) or 0.01025 (high
exposure) according to the experimental data of PAEs (0.000031–0.01025).46 (link) Other parameters included in the equations are
summarized in Table S3.
The hazard
quotient (HQ) was determined to assess human exposure risks via dust
ingestion and dermal absorption. Only chemicals with a DF of >70%
in at least four of the five regions were included for HQ estimation47 where RfD
represents the reference dose of a target chemical. For an analyte
without an appropriate RfD, its nonobserved-adverse-effect-level
(NOAEL) or lethal dose (LD50) adjusted with an uncertainty
factor was applied (Table S4). A hazard
index (HI) was also calculated by summing the HQs for individual analytes.
For a target analyte with a detection frequency (DF) > 70%,
an
LOQ/√2 was assigned to any measurements below the LOQ for statistical
analysis. Statistical analyses and data visualization were conducted
using Origin version 9.0 or PASW Statistics 18.0. Differences among
chemical groups or regions were determined using a Kruskal–Wallis
analyses of variance (ANOVA) followed by a Mann–Whitney test.
Spearman’s correlation analyses were used to determine the
relationships between individual plasticizers in house dust. The level
of significance was set at α = 0.05.
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Publication 2023
Body Surface Area Body Weight Homo sapiens House Dust Nandrolone neuro-oncological ventral antigen 2, human No-Observed-Adverse-Effect Level phenyl-2-aminoethyl sulfide Plasticizers Skin Skin Absorption
We collected 3 mL of venous blood to separate the serum for use and used the Fubok allergen detector and its allergen diagnostic reagent produced by Jiangsu Haooubo Biological Medicine Co., Ltd. (Suzhou, Jiangsu Province, China) to determine the specific IgE of the corresponding allergen in the serum. The system adopts the enzyme-linked immunostaining method to detect the serum-specific IgE content, which is a quantitative detection. Inhalation allergens include house dust mites, dust mites, mugwort, ragweed, cockroaches, cat epithelia, dog epithelia, house dust, Alternaria, and willow. Food allergens include peanut, egg, milk, cod, wheat flour, shrimp, soybean, crab, beef, and mutton.
Publication 2023
Allergens Alternaria Arachis hypogaea Artemisia vulgaris Beef Biopharmaceuticals Brachyura Cockroaches Diagnosis Enzymes Epithelium Food House Dust Inhalation Milk, Cow's Pyroglyphidae Serum Soybeans Veins Wheat Flour Willow
The CCLS is a population-based case-control study in 35 counties in California, including 17 counties in the San Francisco Bay area and 18 in the Central Valley [11 (link),26 (link)]. Between 1995 and 2012, cases ≤14 years old were ascertained within 72 h of diagnosis from nine major pediatric clinical centers in the study area. Using California birth certificate information, controls were matched to cases on the basis of date of birth, sex, Hispanic ethnicity, and maternal race. Parents of both case and control participants were initially interviewed to gather information about their child’s exposure to suspected leukemia risk factors. Families who had not moved since the child’s diagnosis date (reference date for controls) were eligible for a second interview (Tier 2), during which carpet dust samples were collected and information about pesticide use was obtained. The second interview and dust sampling were limited to cases and controls <8 years old (diagnosed December 1999 to June 2006) to ensure the samples reflected early-life chemical exposure of the child. Case-control matching was not maintained due to residential eligibility criteria and voluntary participation. There were 731 participants for the Tier 2 interviews (324 cases and 407 controls). Of these, 296 cases (91%) and 333 controls (82%) agreed to participate. Due to insufficient dust or interferences in the chemical analyses, some samples were not quantified, leading to a final 277 cases and 306 controls (n = 583) [13 (link)].
The dust samples were collected using either a high-volume small surface sampler (HVS3) or a household vacuum cleaner. As described previously [26 (link)], concentrations of 64 organic chemicals (ng/g dust) were measured using gas chromatography/mass spectrometry (GC/MS) in multiple ion-monitoring mode after extraction with three different extraction methods. Nine metals were measured using microwave-assisted acid digestion combined with inductively coupled plasma/mass spectrometry (ICP/MS).
Our analysis investigated the association of 67 chemicals with the risk of childhood leukemia. Out of the entire CCLS dataset, only chemical exposure variables with at least 20% non-missing observations were included, as past experience has shown that higher levels of missingness contribute negligible information on potential relations with an outcome. We organized exposures into seven chemical class indices: polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), insecticides, herbicides, metals, tobacco exposure markers of nicotine and cotinine, and polybrominated diphenyl ethers (PBDEs). The reason for these groupings was that the chemicals share a structural similarity (e.g., PCBs, PAHs, metals) or usage (e.g., herbicides, insecticides). Most missing data were the result of findings below a chemical’s detection limit. Additionally, there were missing data in the PBDE chemicals due to insufficient levels of house dust for analysis. Both types of missing observations were imputed with log-normal distributions, as previously described [15 (link)].
The interviewers took a global positioning system reading of the residence coordinates, which were linked to census data for the computation of the neighborhood deprivation index and used to evaluate spatial random effects for unexplained spatial heterogeneity in leukemia risk.
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Publication 2023
Acids Brominated Diphenyl Ethers Cefaclor Child Childbirth Cotinine Digestion Eligibility Determination Ethnicity Gas Chromatography-Mass Spectrometry Genetic Heterogeneity Herbicides Hispanics House Dust Households Insecticides Interviewers Leukemia Mass Spectrometry Metals Microwaves Mothers Nicotine Organic Chemicals Parent Pesticides Plasma Polychlorinated Biphenyls Polycyclic Hydrocarbons, Aromatic Tobacco Products Vacuum
When the children were 18 months old, JECS staff visited their homes to collect house dust, which contains endotoxin, from the children’s mattresses. The endotoxin concentration was calculated per 1 mg of house dust using a kinetic chromogenic Limulus amebocyte lysate assay (Kinetic–QCL; Lonza Japan, Sagamihara, Japan) [26 (link)].
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Publication 2023
azo rubin S Biological Assay Child endotoxin binding proteins Endotoxins House Dust Kinetics Limulus PER1 protein, human
Pregnant women’s exposures were measured using environmental and biological samples collected in the second trimester (between weeks 12 and 24). Domestic settled dust was used as an environmental indicator to estimate Pb exposure, which represents an indoor source. We calculated the Pb dust loading rate, while the biological samples used were blood (for Cd and Pb), hair (for Mn and As), and toenails (for Mn).
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Publication 2023
Biopharmaceuticals BLOOD Hair House Dust Pregnant Women Toenails

Top products related to «House Dust»

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The Agilent 7900 is an inductively coupled plasma mass spectrometer (ICP-MS) designed for ultra-trace elemental analysis. It offers high sensitivity, stability, and low detection limits for a wide range of elements across diverse sample types.
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The PV-H23 is a laboratory equipment product manufactured by Hitachi. It serves as a power supply unit for various laboratory applications. The core function of the PV-H23 is to provide a stable and adjustable source of electrical power to power other laboratory equipment and devices.
House dust mite protein extract is a laboratory product used for analytical and research purposes. It contains proteins derived from house dust mites, which are a common source of allergens. The extract can be used for various applications, such as the development and testing of diagnostic tests or the study of allergic reactions. The core function of this product is to provide a source of house dust mite proteins for these types of scientific and research activities.
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The Agilent 7500cx is an inductively coupled plasma mass spectrometer (ICP-MS) designed for high-performance elemental analysis. It features advanced technology for sensitive, accurate, and precise measurements of trace elements in a variety of sample types.
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The Kinetic-QCL is a laboratory instrument that utilizes Quantum Cascade Laser (QCL) technology for spectroscopic measurements. It is designed to provide high-precision, real-time analysis of various samples across a wide range of applications.
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The Limulus amebocyte lysate (LAL) assay is a widely used laboratory test that detects and measures the presence of bacterial endotoxins, also known as lipopolysaccharides (LPS). The assay utilizes the blood cells (amebocytes) of the horseshoe crab, Limulus polyphemus, to identify the presence of endotoxins in a sample. This test is a sensitive and reliable method for monitoring the levels of endotoxins in a variety of applications, including pharmaceutical and medical device manufacturing.
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Alkaline phosphatase-conjugated monoclonal anti-human IgE antibodies are a laboratory reagent used in immunoassays to detect and measure the presence of IgE antibodies in biological samples. They are produced by fusing myeloma cells with antibody-producing B cells to create a stable hybridoma cell line that secretes the desired monoclonal antibody. The antibodies are then conjugated with the enzyme alkaline phosphatase, which can be used to generate a measurable signal upon interaction with a suitable substrate.
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Pb(II) acetate, also known as lead(II) acetate, is an inorganic compound with the chemical formula Pb2(C3O2H)2. It is a crystalline solid that is soluble in water and organic solvents. Pb(II) acetate is commonly used as a laboratory reagent and a precursor in the synthesis of other lead compounds.

More about "House Dust"

House dust is a complex mixture of particulate matter, including fibers, microorganisms, and chemicals, commonly found in indoor environments.
Analyzing the composition and characteristics of house dust can provide valuable insights into indoor air quality, potential health risks, and environmental exposures.
Researchers can leverage AI-driven platforms like PubCompare.ai to optimize their house dust studies by identifying the most accurate and reproducible methods from the literature, preprints, and patents.
House dust research can benefit from the use of analytical instruments like the Agilent 7900 ICP-MS, Phadia 250 immunoassay analyzer, and Agilent 7500cx ICP-MS.
These tools can help researchers measure and quantify the presence of various elements, allergens, and contaminants in house dust samples.
Additionally, techniques like the Limulus amebocyte lysate (LAL) assay and the use of alkaline phosphatase-conjugated monoclonal anti-human IgE antibodies can provide insights into the biological and immunological properties of house dust.
To enhance the success of house dust research projects, researchers can leverage AI-driven platforms like PubCompare.ai, which enable data-driven comparisons to identify the most accurate and reproducible methods.
This can include evaluating protocols for dust collection, sample preparation, and analytical techniques, such as the Kinetic-QCL method for endotoxin analysis and the use of GraphPad Prism 5 for data analysis.
By incorporating these insights and tools, researchers can take their house dust studies to the next level, gaining a deeper understanding of indoor air quality, potential health risks, and environmental exposures.
Key subtopics to consider include house dust mite protein extract, Pb(II) acetate (Pb2(C3O2H)2, and the impact of house dust on human health and well-being.