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Thomsen-Friedenreich antibodies

Thomsen-Friedenreich antibodies are a group of carbohydrate-binding proteins that recognise specific glycan structures found on the surface of various cell types, including tumor cells.
These antibodies play a key role in immune surveillance and can be used as biomarkers for cancer detection and monitoring.
PubCompare.ai's AI-driven protocol comparison tool helps researchers optimise their Thomsen-Friedenreich antibody research by identifying the most reproducible protocols from literature, preprints, and patents, providing data-driven insights to enhance reproducibility and accelerate discovery.

Most cited protocols related to «Thomsen-Friedenreich antibodies»

1
Assessing Cdo-APPL1 Complexes in Muscle Development
Cited 8 times
Western blot analyses were carried out as previously described (Bae et al., 2009b (link)). Briefly, cells were lysed in lysis buffer (10 mM Tris-HCl, pH 7.2, 150 mM NaCl/1% Triton X-100/1 mM EDTA) containing proteinase inhibitor (Roche Diagnostics), followed by SDS-PAGE. APPL1 rabbit polyclonal antibody was raised against C-terminal PTB domain of human APPL1. Sera were affinity-purified using Sulfolink beads (Pierce, Rockford, IL). Other antibodies used in this study were as follows: anti-Cdo (Zymed, South San Francisco, CA), anti-SRT (Lee et al., 2001 (link)), anti-HA (Roche Diagnostics), anti-pan-cadherin (Sigma-Aldrich, St. Louis, MO), anti-β-tubulin (Zymed), anti-p38MAPK (Sigma-Aldrich), anti-pp38MAPK, anti-Akt, anti-pAkt (Cell Signaling, Beverly, NA), anti-MHC (MF20; Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA), anti-Troponin T (Sigma-Aldrich), anti-Boc (R&D Systems, Minneapolis, MN), anti-N-cadherin (Zymed), anti-Neogenin (Santa Cruz Biotechnology, Santa Cruz, CA) and anti-GFP (Zymed). To study formation of Cdo-APPL1 complexes, coimmunoprecipitation was performed as described previously (Bae et al., 2009b (link)).
For analysis of dissected hindlimbs by immunoblotting, hindlimb muscles from Cdo+/+ and Cdo−/− mice at E15.5 (embryonic day 15.5) embryos and P1 (postnatal day 1), P3, and P5 mice were pulverized and solubilized with lysis buffer. Lysates were then analyzed by immunoblotting as described above.
Bae G.U., Lee J.R., Kim B.G., Han J.W., Leem Y.E., Lee H.J., Ho S.M., Hahn M.J, & Kang J.S. (2010). Cdo Interacts with APPL1 and Activates AKT in Myoblast Differentiation. Molecular Biology of the Cell, 21(14), 2399-2411.
Publication 2010
Antibodies Buffers Cadherins Cells Co-Immunoprecipitation Diagnosis Edetic Acid Embryo Hindlimb Homo sapiens Hybridomas Immunoglobulins Mitogen-Activated Protein Kinase p38 Mus Muscle Tissue neogenin Protease Inhibitors Rabbits SDS-PAGE Serum Sodium Chloride Thomsen-Friedenreich antibodies Triton X-100 Tromethamine Troponin Troponin T Tubulin Western Blot
2
Immunohistochemical and Immunoblotting Analyses
Cited 8 times
Deparaffinized and rehydrated sections were quenched with 3% hydrogen peroxide and blocked with normal serum. The sections were then incubated with the desired primary antibody (anti-T-antigen, anti-PCNA, anti-Ki-67, anti-E-cadherin, anti-synaptophysin, anti-CD31, anti-cyclinB1, or anti-securin antibody) and washed with Tris-buffered saline followed by incubation with appropriate biotinylated secondary antibody. Characteristic brown color was developed by incubation with 3,3-diaminobenzidine. The sections were counterstained with Meyers Hematoxylin (Sigma) and examined under a Leica microscope. At least three non-overlapping representative images of each tissue were captured from each section using a camera mounted onto the microscope. The images were analyzed using Image ProPlus 5.0 software (Media Cybernetics) for quantitation of PCNA, Ki-67, E-cadherin, T-antigen, cyclinB1, and securin expression and analysis of microvessel number and vessel diameter (CD31 staining). Immunoblotting for T-antigen expression using prostate/tumor tissue supernatants from control and DATS-treated mice was performed as previously described by us for other proteins (27 (link)).
Singh S.V., Powolny A.A., Stan S.D., Xiao D., Arlotti J.A., Warin R., Hahm E.R., Marynowski S.W., Bommareddy A., Potter D.M, & Dhir R. (2008). Garlic Constituent Diallyl Trisulfide Prevents Development of Poorly-Differentiated Prostate Cancer and Pulmonary Metastasis Multiplicity in TRAMP Mice. Cancer research, 68(22), 9503-9511.
Publication 2008
Alzheimer's Disease anti-synaptophysin Antibodies, Anti-Idiotypic Antigens Blood Vessel Cadherins CDH1 protein, human Hematoxylin Immunoglobulins Microscopy Microvessels Mus Peroxide, Hydrogen Proliferating Cell Nuclear Antigen Prostatic Neoplasms Proteins PTTG1 protein, human Saline Solution Serum Thomsen-Friedenreich antibodies Tissues Viral Tumor Antigens
3
Immunostaining of T. gondii Infection
Cited 8 times
Serological reagents used were: Anti-IIGP1 165 rabbit antiserum [35 (link)], anti-IIGP1 10E7, and 10D7 mouse monoclonal antibodies (mAb), anti-IGTP I68120 mAb (BD Transduction Laboratories, Lexington, Kentucky, United States), anti-TGTP1 A20 goat antiserum (Santa Cruz Biotechnology, Santa Cruz, California, United States), anti-LRG-47 A19 goat antiserum (Santa Cruz), anti-GTPI H53 rabbit antiserum raised against the N-terminal peptide MEEAVESPEVKEFEY, anti-IRG-47 2078 rabbit antiserum raised against the peptides CKTPYQHPKYPKVIF, and CDAKHLLRKIETVNVA, anti-T. gondii rabbit antiserum (BioGenex, San Ramon, California, United States), anti-LAMP1 1D4B rat mAb (University of Iowa, Iowa City, Iowa, United States), anti-GRA7 5–241–178 mouse mAb (gift from R. Ziemann, Abbott Laboratories, Abbot Park, Illinois, United States) [41 (link)], anti-ROP2/3/4 T24A7 mouse mAb (gift of J. Dubremetz, Montpellier, France) [59 (link)], anti-ctag1 2600 rabbit antiserum raised against the peptide CLKLGRLERPHRD, anti-ERP60 rabbit antiserum (gift from T. Wileman, BBSRC, Pirbright, United Kingdom), SPA-265 anti-calnexin rabbit antiserum (Stressgene), anti-PDI mAb (BD Transduction Laboratory), anti-Gm130 mAB (BD Transduction Laboratory), goat anti-mouse Alexa 546/488, goat anti-rabbit Alexa 546/488, donkey anti-goat Alexa 546/488, donkey anti-mouse Alexa 488, donkey anti-rabbit Alexa 488, donkey anti-rat Alexa 488, goat anti-rabbit Alexa 680 (Molecular Probes, Eugene, Oregon, United States).
Martens S., Parvanova I., Zerrahn J., Griffiths G., Schell G., Reichmann G, & Howard J.C. (2005). Disruption of Toxoplasma gondii Parasitophorous Vacuoles by the Mouse p47-Resistance GTPases. PLoS Pathogens, 1(3), e24.
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Publication 2005
Anti-Antibodies Calnexin Calreticulin Equus asinus Goat Immune Sera lysosomal-associated membrane protein 1, human Mice, House Molecular Probes Peptides Rabbits Thomsen-Friedenreich antibodies
4
Exploring Cardioprotective Mechanisms of Irisin
Cited 7 times
Antibodies against the following proteins were purchased from Cell Signaling Technology (Danvers, MA, USA): BAX (1:1000), cleaved-Caspase3 (C-Caspase, 1:1000), total Caspase3 (T-Caspase3, 1:1000), total AKT (T-AKT, 1:1000), phosphorylated AKT (P-AKT, 1:1000), T-mTOR (1:1000), P-mTOR (1:1000), T-P70 (1:1000), P-P70 (1:1000), T-ribosomal protein S6 (T-S6, 1:1000), P-S6 (1:1000), T-4EBP1 (1:1000), P-4EBP1 (1:1000), T-glycogen synthase kinase 3β (T-GSK3β, 1:1000), P-GSK3β (1:1000), 4-Hydroxynonenal (4-HNE, 1:200 for staining), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, 1:1000). Antibodies for FNDC5 (1:1000 for western blot, 1:100 for staining), p67phox (1:1000), superoxide dismutase 1 (SOD1, 1:1000), SOD2 (1:1000), B-cell lymphoma 2 (BCL-2, 1:1000), Nrf2 (1:1000), heme oxygenase-1 (HO-1, 1:1000), Kelch-like ECH-associated protein 1 (Keap1, 1:1000), and heat shock protein 20 (HSP20, 1:1000) were purchased from Abcam (Cambridge, UK). Anti-T-FYN (1:200), anti-P-FYN (1:200), and anti-T-proliferating cell nuclear antigen (PCNA, 1:200) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). The secondary antibody used for western blot was purchased from LI-COR Biosciences, whereas anti-rabbit/mouse EnVisionTM+/HRP reagent used for immunohistochemistry was obtained from Gene Technology (Shanghai, China). DOX, irisin, AKT inhibitor (AKT i), rapamycin (Rapa) and dexrazoxane (DEX) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dihydroethidium (DHE) was obtained from Keygen Biotech, and 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), malondialdehyde (MDA) assay kit, glutathione (GSH) assay kit, total SOD assay kit and NADPH oxidase assay kit were all purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Phosphoinositide 3-kinase (PI3K) activity ELISA assay kit was obtained from Echelon Biosciences Inc. ApopTag® Plus In Situ Apoptosis Fluorescein Detection Kit was purchased from Millipore (Billerica, MA, USA) and the cell counting kit-8 (CCK-8) was obtained from Dōjindo Laboratories (Kumamoto, Japan).
Zhang X., Hu C., Kong C.Y., Song P., Wu H.M., Xu S.C., Yuan Y.P., Deng W., Ma Z.G, & Tang Q.Z. (2019). FNDC5 alleviates oxidative stress and cardiomyocyte apoptosis in doxorubicin-induced cardiotoxicity via activating AKT. Cell Death and Differentiation, 27(2), 540-555.
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Publication 2019
1-Phosphatidylinositol 3-Kinase 4-hydroxy-2-nonenal Antibodies Apoptosis B-Cell Lymphomas BCL2 protein, human Biological Assay Caspase Caspase 3 Dexrazoxane dihydroethidium EIF4EBP1 protein, human Enzyme-Linked Immunosorbent Assay Fluorescein FRAP1 protein, human GAPDH protein, human Genes Glyceraldehyde-3-Phosphate Dehydrogenases GSK3B protein, human Heat Shock Proteins HMOX1 protein, human Immunoglobulins Immunohistochemistry KEAP1 protein, human Malondialdehyde Mus NADPH Oxidase neutrophil cytosol factor 67K NFE2L2 protein, human Proliferating Cell Nuclear Antigen Proteins Rabbits Ribosomal Protein S6 Sirolimus SOD2 protein, human Superoxide Dismutase-1 Thomsen-Friedenreich antibodies Western Blotting
5
Discriminating Tissue-Resident CD8 T Cells
Cited 7 times
To discriminate between CD8 T cells in tissue parenchyma versus tissue vasculature, i.v. injected Ab was used as previously described (28 (link)). Briefly, 3μg of anti-CD8α Ab (53-6.7, Biolegend, San Diego, CA) was injected i.v. and allowed to circulate for three minutes prior to mouse sacrifice.
Organs were harvested and digested as previously described (29 (link)). For isolation of small intestinal intraepithelial lymphocytes (IEL), Peyer’s patches were removed, the small intestine was cut longitudinally and then laterally into small pieces. Pieces were incubated for 30 minutes with stirring at 37°C with 0.154mg/mL dithioerythritol (Sigma-Aldrich, St. Louis, MO) in 10% HBSS/HEPES. Female reproductive tract (FRT), lung and salivary gland (SG) tissues were cut into small pieces in RPMI 1640 containing 5% FBS, 2 mM MgCl2, 2 mM CaCl2 and 0.5mg/mL type IV collagenase for FRT (Sigma-Aldrich, St. Louis, MO) or 100 U/mL type I collagenase for lung and SG (Worthington, Lakewood, NJ) and incubated for 1 hr at 37°C with stirring. After enzymatic digestion, the remaining tissue pieces were mechanically disrupted using a gentleMACs dissociator (Miltenyi Biotec, San Diego, CA). The liver was mechanically dissociated by pushing the tissue through a 70μm-cell strainer. Single cell suspensions of IEL, FRT, lung, liver and SG were further separated using a 44/67% Percoll (GE Healthcare Life Sciences, Pittsburgh, PA) density gradient. Spleen and lymph nodes (LN) were dissociated mechanically. Splenocytes and blood were treated with ACK lysis buffer to lyse red blood cells.
The following antibodies were used for flow cytometry: anti-KLRG1 (2F1), anti-Eomes (Dan11ma), anti-T-bet (4B10), anti-CD44 (IM7), anti-CD122 (TM-b1), anti-CD27 (LG.7F9), anti-CD69 (H1.2F3) (all from eBioscience, San Diego, CA), anti-CD8α (53-6.7, eBioscience, Biolegend, San Diego, CA), anti-CD103 (M290), anti-CD25 (PC61), anti-Bcl-2 (Bcl-2/100) and anti-CD127 (SB/199) (BD Biosciences, San Jose, CA). Cell viability was determined using Ghost DyeTM Red 780 (Tonbo Biosciences, San Diego, CA). Kb-SIINFEKL-specific CD8 T cells were identified using H-2Kb tetramers made in house containing the SIINFEKL peptide (New England Peptide, Gardener, MA). The BD Biosciences intracellular kit for cytokine staining and the eBioscience FoxP3 kit for transcription factor staining were used in accordance with manufacturer’s directions. Peptide stimulation was performed as previously described (30 (link)). Briefly, splenocytes were plated in RPMI 1640 containing 10% FBS, 1× NEAA, 2mM L-glutamine, 1mM sodium pyruvate, 1× penicillin/streptomycin and 0.05mM β-mercaptoethanol and incubated with 1ug/mL SIINFEKL peptide and 1ug/mL GolgiPlug (BD Biosciences, San Jose, CA) for four hours at 37°C. Cells were washed and stained with fixable LIVE/DEAD aqua dead cell stain (Life Technologies, San Diego, CA) before surface and intracellular staining. Samples were acquired on an LSRII flow cytometer (BD Biosciences, San Diego, CA).
Thompson E.A., Beura L.K., Nelson C.E., Anderson K.G, & Vezys V. (2016). Shortened intervals during heterologous boosting preserve memory CD8 T cell function but compromise longevity. Journal of immunology (Baltimore, Md. : 1950), 196(7), 3054-3063.
Publication 2016
2-Mercaptoethanol alpha HML-1 Antibodies BCL2 protein, human BLOOD Buffers CD8-Positive T-Lymphocytes CD44 protein, human Cells Cell Survival Collagenase, Clostridium histolyticum Cytokine Digestion Dithioerythritol Enzymes Erythrocytes Female Reproductive System Flow Cytometry Glutamine Hemoglobin, Sickle HEPES IL2RA protein, human IL2RB protein, human Intestines, Small Intraepithelial Lymphocytes isolation KB Cells KLRG1 protein, human Liver Lung Lymphocyte Magnesium Chloride Matrix Metalloproteinase 2 Mus Nodes, Lymph OVA-8 Penicillins Peptides Percoll Peyer Patches Protoplasm Pyruvate Red Cell Ghost Salivary Glands Sodium Spleen Stains Streptomycin Tetrameres Thomsen-Friedenreich antibodies Tissues Transcription Factor

Most recents protocols related to «Thomsen-Friedenreich antibodies»

1
CD28-CA125-PD1 VSV-G Virus Activation and Inhibition
Not available on PMC !

Example 3

Human T cells are infected with the pseudotyped CD28-CA125-PD1 VSV-G virus. 24 hrs to 48 hrs post viral infection, the T cell culture medium is collected and checked for the presence of proinflammatory cytokines. These results will show that T cells are activated by CD28-CA125-PD1 VSV-G, as evidenced by presence of proinflammatory cytokines such as IFN-β and IL-2 in the cell culture supernatant of CD28-CA125-PD1 VSV-G infected human T cells.

EphA2-overexpressing gastric cancer cells, from KATO3 cell line, are infected with pseudotyped CD28-CA125-PD1 VSV-G or non-pseudotyped CD28-CA125-PD1 VSV virus and the cell proliferation is assessed. These results will show that cell proliferation is significantly reduced in cells KATO3 cells infected with pseudotyped CD28-CA125-PD1 VSV-G compared to KATO3 cells infected with non-pseudotyped CD28-CA125-PD1 VSV virus.

US11865081B2. Oncolytic viral delivery of therapeutic polypeptides (2024-01-09). Virogin Biotech Canada Ltd. [CA]. Inventors: Mitchell H. Finer [US], Luke Evnin [US].
Full text: Click here
Patent 2024
CA-125 Antigen Cell Lines Cell Proliferation Cells Culture Media Cytokine Gastric Cancer Gastrin-Secreting Cells Homo sapiens Neoplasms T-Lymphocyte Thomsen-Friedenreich antibodies Virus Virus Diseases
2
GPRC5D-Targeted CAR T Cell Therapy for Myeloma
Not available on PMC !

Example 8

GPRC5D targeted 28z CAR18 T cells mediated an anti-myeloma immune response. 1×107 U266 human myeloma cell line cells were injected IV into NSG mice on day 0. On day 4 1×106 GPRC5D targeted or CD19 targeted second generation CAR T cells were injected IV. Imaging on day 11 (day 7 s/p CAR T cell injection) shows that, unlike irrelevant (CD19) targeted CAR T cells; GPRC5D A targeted 28z CAR18 T cells can mediate an anti-tumor response. See FIG. 12.

US11866478B2. Nucleic acid molecules encoding chimeric antigen receptors targeting G-protein coupled receptor (2024-01-09). MEMORIAL SLOAN KETTERING CANCER CENTER [US], EUREKA THERAPEUTICS, INC. [US]. Inventors: Renier J. Brentjens [US], Eric L Smith [US], Cheng Liu [US].
Full text: Click here
Patent 2024
Cell Lines Cells GPRC5D protein, human Homo sapiens Multiple Myeloma Mus Neoplasms Response, Immune T-Lymphocyte Thomsen-Friedenreich antibodies
3
Bivalent Binding of HER2 Inhibits Proliferation
Not available on PMC !

Example 8

Binding of trastuzumab to overexpressed HER2 in HER2 amplified cancer cells results in robust interference of constitutive ligand independent PI3K signaling initiated by the deregulated HER2-HER3 complex (Junttila et al. Cancer Res. 74(19): 5561-5571, 2014). The 1Fab-IgG TDB molecule binds to HER2 in bivalent form. In vitro treatment of SKBR3 cells with incubation with 1Fab-IgG TDB resulted in transient non-durable reduction of pAKT, without detectable effect on HER3 phosphorylation (FIG. 28A). In addition, neither monovalent high HER2 affinity IgG TDB, nor bivalent low HER2 affinity 1Fab-IgG TDB substantially affected the proliferation/viability of SKBR3 cells (FIG. 28B). In summary, bivalent low affinity binding to HER2 does not inhibit proliferation of HER2-amplified cells.

US11866498B2. Bispecific antigen-binding molecules and methods of use (2024-01-09). Genentech, Inc. [US]. Inventors: Diego Ellerman [US], Teemu T. Junttila [US], Twyla Noelle Lombana [US], Dionysos Slaga [US], Christoph Spiess [US].
Full text: Click here
Patent 2024
Cardiac Arrest Cell Proliferation Cells Cell Survival erbB-3, Proto-Oncogene Proteins ERBB2 protein, human Ligands Malignant Neoplasms Phosphorylation PIK3CB protein, human Thomsen-Friedenreich antibodies Transients Trastuzumab
4
Anti-GD2 CAR T-cell Therapy for CT26 Tumors
Not available on PMC !

Example 9

CT26 cell line was engineered to express GD2 as described above (designated CT26 clone #7 or CT25#7 for short). Either 2×105 of wild type (wt) or GD2 positive CD26 cells were inoculated into the flanks of C57BL/6 mice (syngeneic with CT26). 10 days after tumour challenge, mock-transduced and anti-GD2 CAR transduced syngeneic splenocytes were prepared. Mice were divided into the following 4 cohorts: mice with GD2 expressing CT26 tumours receiving anti-GD2 CAR spleoncytes; GD2 expressing CT26 tumours receiving mock-transduced splenocytes; GD2 negative (wt) CT26 tumours with anti-GD2 CAR splenocytes; and GD2 expressing CT26 tumours receiving no splenocytes. Tumour was measured using a digital caliper in 3 dimension and volume estimated therewith. FIG. 11 shows the growth curves of the tumours. Only GD2 positive tumours in mice receiving anti-GD2 CAR T-cells had little or no growth.

US11879016B2. Chimeric antigen receptor (2024-01-23). AUTOLUS LIMITED [GB]. Inventors: Martin Pulé [GB], John Anderson [GB], Simon Thomas [GB].
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Patent 2024
Cells Clone Cells DPP4 protein, human Mice, Inbred C57BL Mus Neoplasms Physiology, Cell Thomsen-Friedenreich antibodies
5
Comprehensive Splenocyte Immunophenotyping Protocol
Splenocytes were prepared by manual disruption of spleens into cold staining medium (1× PBS + 3% calf serum + 5 mM EDTA + 0.05% sodium azide) followed by red blood cell lysis with ammonium chloride/potassium chloride (ACK) solution. Single-cell suspensions were washed with PBS and labeled with Ghost 510 live/dead reagent (Tonbo). Washed splenocytes were blocked with unconjugated anti-CD16/32 (clone 2.4G2) in staining medium and then stained with surface antibody cocktails on ice. Following washing and secondary staining with streptavidin-conjugated fluorophores where necessary, cells were washed in staining medium and fixed with 1% paraformaldehyde in 1× PBS. For T-bet intracellular staining, cells were permeablized with 0.1% Triton-X 100 in staining medium for 20 min on ice, then washed and blocked with staining medium containing 10% mouse serum and incubated with anti–T-bet antibody overnight. For Ki67 intracellular staining, cells were first fixed in 2% paraformaldehyde in 1× PBS then permeabilized with 1× Foxp3 Perm/Wash buffer (eBiosciences) for 30 min on ice, washed with Foxp3 Perm/Wash buffer, blocked with Foxp3 Perm/Wash buffer supplemented with 10% rat serum, then stained with anti-Ki67 antibody overnight. Following overnight incubations, cells were washed twice with Perm/Wash then twice with staining medium. Data were collected on a Becton Dickinson LSR II or Fortessa and analyzed in FlowJo.
Antibodies and flow cytometry reagents used in this study were: CD8-Alexa647 (TIB105), CD11c-Alexa488 (N418), CD11c-Alexa647 (N418), CD21/35-Alexa647 (7G6), CD44-Alexa488 (Pgp1), CD44-Alexa647 (Pgp1), CD45.1-Alexa647 (A20), CD45.2-Alexa488 (104), CD317-Alexa647 (eBio927)—lab grown from hybridoma and fluorophore conjugated; PNA-Alexa647 (Vector labs)—lab conjugated; CD4-BV421 (GK1.5), CD4-PE (GK1.5), CD8a-APC-Cy7 (53-6.7), CD11c-PE-Cy7 (N418), CD11b-APC-Cy7 (M1/70), CD11b-PE (M1/70), CD21/35-PerCP-Cy5.5 (7E9), CD23-PE-Cy7 (B3B4), CD38-PE (90), CD44-APCCy7 (IM7), CD62L-APC-eFluor780 (Mel-14), CD93-PE (AA4.1), CD138-BV605 (281-2), CD138-PE (281-2), I-A/I-E-BV605 (M5/114.15.2), T-bet-PE (4B10), TCRβ-PerCP-Cy5.5 (H57-597), TCRβ-BV421 (H57-597)—Biolegend; CD11b-BUV737 (M1/70), CD19-BUV395 (1D3), CD44-BV605 (1M7), CD73-PE (TY/11.8), CD80-BV421 (16-10A1), CD138-BV605 (281-2), CD273-biotin (TY25), SiglecH-BUV737 (440c), Streptavidin-BUV737—Becton Dickinson; CD45.1-APC-eFluor780 (A20), Ki67-FITC (SolA15), BrdU-Alexa647 (Mobu1)—eBiosciences/Invitrogen. All antibodies were titrated prior to use.
Nickerson K.M., Smita S., Hoehn K.B., Marinov A.D., Thomas K.B., Kos J.T., Yang Y., Bastacky S.I., Watson C.T., Kleinstein S.H, & Shlomchik M.J. (2023). Age-associated B cells are heterogeneous and dynamic drivers of autoimmunity in mice. The Journal of Experimental Medicine, 220(5), e20221346.
Publication 2023
Alexa Fluor 647 Antibodies Antibodies, Anti-Idiotypic Antigen T Cell Receptor, beta Chain Biotin Bromodeoxyuridine BST2 protein, human Buffers CD44 protein, human Cells Chloride, Ammonium Clone Cells Cloning Vectors Common Cold CY5.5 cyanine dye Edetic Acid Erythrocytes Flow Cytometry Fluorescein-5-isothiocyanate Hybridomas Immunoglobulins ITGAM protein, human Mus NT5E protein, human paraform Potassium Chloride Progressive Encephalomyelitis with Rigidity Protoplasm Receptors, Antigen, B-Cell Red Cell Ghost SDC1 protein, human SELL protein, human Serum Sodium Azide Streptavidin Thomsen-Friedenreich antibodies Triton X-100

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1
4 6 diamidino 2 phenylindole (dapi) by Thermo Fisher Scientific
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DAPI is a fluorescent dye used in microscopy and flow cytometry to stain cell nuclei. It binds strongly to the minor groove of double-stranded DNA, emitting blue fluorescence when excited by ultraviolet light.
2
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The FACSCalibur is a flow cytometry system designed for multi-parameter analysis of cells and other particles. It features a blue (488 nm) and a red (635 nm) laser for excitation of fluorescent dyes. The instrument is capable of detecting forward scatter, side scatter, and up to four fluorescent parameters simultaneously.
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The LSRFortessa is a flow cytometer designed for multiparameter analysis of cells and other particles. It features a compact design and offers a range of configurations to meet various research needs. The LSRFortessa provides high-resolution data acquisition and analysis capabilities.
4
Foxp3 transcription factor staining buffer set by Thermo Fisher Scientific
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The Foxp3/Transcription Factor Staining Buffer Set is a laboratory product designed for the detection and analysis of intracellular transcription factors, such as Foxp3, using flow cytometry. The set includes the necessary buffers and reagents to facilitate the fixation, permeabilization, and staining of cells for the purpose of intracellular protein detection and quantification.
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The Cytofix/Cytoperm kit is a laboratory product designed for fixing and permeabilizing cells. It provides the necessary solutions for the preparation of samples prior to intracellular staining and flow cytometric analysis.
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Image Lab software is a data analysis tool designed for use with Bio-Rad's gel and blot imaging systems. The software provides a user-friendly interface for capturing, analyzing, and processing images of gels, blots, and other samples.
10
Ab8295 by Abcam
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Ab8295 is a rabbit monoclonal antibody that recognizes the Histidine (His) tag. It is intended for use in various laboratory techniques, including immunoassays and Western blotting, to detect and monitor proteins with a His tag.

More about "Thomsen-Friedenreich antibodies"

Thomsen-Friedenreich (TF) antibodies are a group of carbohydrate-binding proteins that recognize specific glycan structures found on the surface of various cell types, including tumor cells.
These antibodies play a crucial role in immune surveillance and can be used as biomarkers for cancer detection and monitoring.
TF antibodies are of great interest in cancer research as they can bind to the TF antigen, which is overexpressed on the surface of many cancer cells.
The TF antigen is a disaccharide (Galβ1-3GalNAc) that is typically hidden by larger glycan structures in healthy cells but becomes exposed on the surface of tumor cells.
The detection and study of TF antibodies often involve techniques like DAPI staining, flow cytometry using FACSCalibur or LSRFortessa instruments, and Western blotting with PVDF membranes.
Researchers may also use the Foxp3/Transcription Factor Staining Buffer Set or the Cytofix/Cytoperm kit to permeabilize cells and detect intracellular TF antigens.
Bovine serum albumin (BSA) is commonly used as a blocking agent in these assays.
To optimize their TF antibody research, scientists can utilize PubCompare.ai's AI-driven protocol comparison tool.
This tool helps researchers identify the most reproducible protocols from literature, preprints, and patents, providing data-driven insights to enhance reproducibility and accelerate discovery.
By leveraging the information in this tool, researchers can more effectively study the role of TF antibodies in cancer and develop new diagnostic and therapeutic approaches.
Additionally, the use of ionomycin, a calcium ionophore, can induce the expression of the TF antigen on the cell surface, which can be useful for studying the binding and functionality of TF antibodies.
The Image Lab software can also be employed for the analysis and visualization of TF antibody-related data.