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Alexa594

Alexa Fluor 594 is a fluorescent dye commonly used in biological research.
It is a member of the Alexa Fluor dye series, which are known for their bright fluorescence and photostability.
Alexa Fluor 594 has an excitation maximum at 590 nm and an emission maximum at 617 nm, making it compatible with common red fluorescent protein and rhodamine filter sets.
This dye is frequently conjugated to antibodies, proteins, or other biomolecules to enable visualization and detection in a variety of assays, including immunohistochemistry, flow cytometry, and live-cell imaging.
Alexa Fluor 594 is a valuable tool for researchers studying cellular processes, protein localization, and molecular interactions.
Its versatility and performance make it a popular choice for fluorecsent labeling and detection in the life sciences.

Most cited protocols related to «Alexa594»

Transwell Invasion and ECM Degradation Assay

Transwell invasion assay was performed as described previously^{4 (link)}. In brief, cells were loaded onto the upper well of the Transwell chamber with 8 µm ϕ pore membrane (Coster), precoated with Matrigel on an upper side of the chamber. The lower well was filled with 600 µl of DMEM containing 10% FBS. After incubation for 24 hr, cells invaded to lower surface of the membrane were counted. For ECM degradation assay, glass coverslips were coated with gelatin conjugated with either Alexa Fluor 594 (Invitrogen) (Alexa-gelatin) or fluorescein (Invitrogen) (FL-gelatin) as described^{65 (link)}. Transfected cells were trypsinized, replated on these glass coverslips, and cultured for 6 hr. After fixation, cells were fixed and stained with phalloidin. Number of invadopodia, identified as F-actin dots in the areas of degraded gelatin, was quantified by using the ImageJ particle analysis tool.

Nishita M., Park S.Y., Nishio T., Kamizaki K., Wang Z., Tamada K., Takumi T., Hashimoto R., Otani H., Pazour G.J., Hsu V.W, & Minami Y. (2017). Ror2 signaling regulates Golgi structure and transport through IFT20 for tumor invasiveness. Scientific Reports, 7, 1.

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Publication 2017

Alexa594 Biological Assay Cells F-Actin Fluorescein Gelatins matrigel Phalloidine Podosomes Tissue, Membrane

In vivo Cell-Attached Electrophysiology

In vivo cell-attached recordings were performed using glass pipettes (∼5-7 MΩ) filled with solution containing the following (in mM): 125 NaCl, 5 KCl, 10 glucose, 10 HEPES, 2 CaCl₂, 2 MgSO₄, and 0.1 Alexa Fluor 594; pH 7.4). Signals were amplified using an AxoPatch 200B amplifier (Molecular Devices), filtered at 5 kHz, and digitized at 10 kHz. Spikes were recorded using current clamp mode. The frame trigger pulses of ScanImage 4.0 were also recorded and used offline to synchronize individual frames to electrophysiological recordings. After establishment of a low-resistance seal (15-50 MΩ), the orientation, spatial and temporal frequency of the stimuli was quickly optimized for individual neurons using recorded spikes. The optimal grating stimulus was repeated at a reduced contrast to maintain a moderate spiking rate.

Chen T.W., Wardill T.J., Sun Y., Pulver S.R., Renninger S.L., Baohan A., Schreiter E.R., Kerr R.A., Orger M.B., Jayaraman V., Looger L.L., Svoboda K, & Kim D.S. (2013). Ultra-sensitive fluorescent proteins for imaging neuronal activity. Nature, 499(7458), 295-300.

Publication 2013

Alexa594 Cells Glucose HEPES Medical Devices Neurons Phocidae Precipitating Factors Pulses Reading Frames Sodium Chloride Sulfate, Magnesium

In vivo Cell-Attached Electrophysiology

Publication 2013

Alexa594 Cells Glucose HEPES Medical Devices Neurons Phocidae Precipitating Factors Pulses Reading Frames Sodium Chloride Sulfate, Magnesium

Molecular Dynamics Simulation with Langevin Integrator

Molecular dynamics was
propagated via the Langevin dynamics integrator in GROMACS (version
4.5.5 or 4.6.5),³⁰ using a time step of
2 fs, and a friction coefficient of 1 ps^–1. Langevin
dynamics was used because it is a very effective thermostat and correctly
samples the canonical ensemble. The friction used here will have only
a small effect on the dynamics,³¹ and no
effect on most of the observables we are concerned with, because these
are almost all equilibrium configurational averages. Lennard-Jones
pair interactions were cut off at 1.4 nm, electrostatic energies were
computed via particle-mesh Ewald³² with
a grid spacing of ∼0.1 nm and a real-space cutoff of 0.9 nm.
The force field in all cases was a derivative of Amber ff03:^{33 (link)} either Amber ff03*^{6 (link)} in conjunction with the TIP3P water model¹¹ or Amber ff03w^{20 (link)} in conjunction with
the TIP4P/2005 water model.^{13 (link)} The force
field for the chromophores Alexa 488 and Alexa 594 will be described
in a future publication, and has been extensively validated against
experimental data.
In certain cases, temperature replica exchange
simulations were performed (for Csp M34, ACTR, Ac-(AAQAA)₃-NH₂, GB1 hairpin, chignolin, and Trp cage). The protocol
for these is the same as above, with exchanges attempted every 1 ps.
Further details on the temperature ranges and simulation lengths for
each case are included in the Results section.
We eliminate from the analysis any configurations in which the proteins
make van der Waals contact with their periodic image, defined by a
closest approach of any atom with an image atom of less than 0.3 nm.²²Nativeness of proteins and peptides was
assessed by computing the d_RMS over native
contacts, defined as the mean-square
difference between the distances d_ij⁰ between residue pairs (i,j) in contact in the reference (native)
state, and the corresponding distance d_ij(x) in a given configuration x The list of the N_ij native contacts, {native}, is defined
as all pairs
of heavy atoms (i,j) within 4.5
Å in the native structure, excluding pairs for which |Res(i) – Res(j)| ≤ 2, where the
function Res(k) gives the residue number of atom k.

Best R.B., Zheng W, & Mittal J. (2014). Balanced Protein–Water Interactions Improve Properties of Disordered Proteins and Non-Specific Protein Association. Journal of Chemical Theory and Computation, 10(11), 5113-5124.

Publication 2014

Alexa594 Amber chignolin Desminopathy, Primary Electrostatics Friction Molecular Dynamics Peptides Proteins

Quantification of GCaMP Expression in Neurons

Adult mice (P42–P56) were deeply anesthetized with isofluorane and transcardially perfused with 10 ml 1× Dulbecco's phosphate-buffered saline (DPBS, Life Technologies), followed by 50 ml 4% paraformaldehyde in 0.1 M phosphate buffer. After perfusion, the brains were removed and post-fixed overnight at 4°C. The brains were embedded in 5% agarose in DPBS, and cut into 50 µm thick coronal sections with a vibratome (Leica VT 1200S). Since DPBS contains a saturating concentration of calcium (0.9 mM) GCaMP brightness will be maximal. Every other section was dehydrated with DPBS and coverslipped with Vectashield mounting medium (H-1400, Vector laboratories). The coverslipped sections were imaged using a slide scanner (Nanozoomer, Hamamatsu). Confocal images (LSM 710, Zeiss) were collected for selected brain regions (Fig. 1 and 2, Fig. S1 and S3) [26] , using an 20× 0.8 NA objective and standard GFP imaging filters. Individual images were tiled and stitched using commercial software (Zeiss).
For a subset of mouse lines (GP4.3, GP4.12, GP5.5, GP5.11, and GP5.17) we visualized neurons using NeuN to measure the fraction of neurons expressing GCaMP. Staining was performed on sections that were not used for quantification of expression. Sections were blocked with 2% BSA and 0.4% Triton X-100 solution for 1 hour at room temperature to prevent nonspecific antibody binding, followed by incubation overnight at 4°C with mouse anti-NeuN primary antibody (1∶500; Millipore, MAB 377) and incubation with Alexa594-conjugated goat-anti-mouse secondary antibody (1∶ 500; Life Technologies, A11032) for 4 hours at room temperature. Sections were mounted on microscope slides with Vectashield mounting medium (H-1400, Vector laboratories).
We analyzed primary motor cortex (M1), primary somatosensory cortex (S1), primary visual cortex (V1) and hippocampus (CA1, CA3, and Dentate Gyrus, DG) using confocal microscopy. For sample images in each area we identified all labeled cells, segmented their somata, and calculated the somatic GCaMP fluorescence brightness for each cell. For cortical regions, cells were grouped into layer 2/3 (L2/3) and layer 5 (L5) cells. We also counted the fraction of GCaMP labeled cells (green channel) as a fraction of the NeuN stained cells (red channel). To compensate for variations of imaging conditions across time (e.g. changes in the excitation light source intensity), images of a fluorescence standard, 3.8 µm fluorescent beads (Ultra Rainbow Fluorescent Particles, Bangs Laboratories), were acquired. The average bead brightness was used to normalize the GCaMP signal.
In addition we performed a coarse analysis of expression levels across numerous brain regions (Table 1; Data S1).

Dana H., Chen T.W., Hu A., Shields B.C., Guo C., Looger L.L., Kim D.S, & Svoboda K. (2014). Thy1-GCaMP6 Transgenic Mice for Neuronal Population Imaging In Vivo. PLoS ONE, 9(9), e108697.

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Publication 2014

Adult Alexa594 anti-c antibody Antibodies, Anti-Idiotypic Brain Buffers Calcium Carisoprodol Cells Cloning Vectors Cortex, Cerebral Diploid Cell Fluorescence Goat Gyrus, Dentate Immunoglobulins Light Microscopy Microscopy, Confocal Motor Cortex, Primary Mus Neurons paraform Perfusion Phosphates Saline Solution Seahorses Sepharose Somatosensory Cortex, Primary Triton X-100

Most recents protocols related to «Alexa594»

Immunofluorescence Analysis of Muscle Tissue

The muscles were cut on a cryostat at − 23 °C (7 μm), air-dried, and stored at − 20 °C. Slides were air-dried, rehydrated, and fixed in 4% paraformaldehyde (PFA) for 20 min at the time of staining. For CD63/DAPI/laminin staining, sections were incubated with mouse anti-CD63 IgG1 antibody (1:100 dilution, ab108950, Abcam, Cambridge, UK) and rabbit anti-laminin IgG antibody (1:100 dilution, L9393, Sigma-Aldrich, St. Louis, MO) overnight at 4 °C. Slides were washed in PBS, then incubated with Alexa Fluor 488 goat anti-mouse IgG1 (1:250 dilution, A11001, Invitrogen, Waltham, MA) and Alexa Fluor 594 goat anti-rabbit IgG (1:250 dilution, A11012, Invitrogen) secondary antibodies for 1 h at room temperature. Slides were washed in PBS and mounted with VectaShield fluorescent mounting media with DAPI (H-1200-10, Vector Laboratories, Newark, CA). For CD9/DAPI/dystrophin staining, sections were incubated with rabbit anti-CD9 IgG (1:100 dilution, SA35-08, Invitrogen) and mouse anti-dystrophin IgG2b (1:250 dilution, 08168, Sigma-Aldrich) overnight, followed by incubation with Alexa Fluor 594 goat anti-rabbit IgG (1:250 dilution, A11012, Invitrogen) and Alexa Fluor 647 goat anti-mouse IgG2b (1:250 dilution, A32728, Invitrogen) for 1 h at room temperature. For CD81/DAPI/dystrophin staining, sections were incubated with rabbit anti-CD81(1:100 dilution, SN206-01, Novus Biologicals, Centennial, CO) and mouse anti-dystrophin IgG2b (1:250 dilution, 08168, Sigma-Aldrich) overnight, followed by incubation with Alexa Fluor 594 goat anti-rabbit IgG (1:250 dilution, A11012, Invitrogen) and Alexa Fluor 647 goat anti-mouse IgG2b (1:250 dilution, A32728, Invitrogen) for 1 h at room temperature. For Pax7/CD9/DAPI/WGA staining, sections were subjected to epitope retrieval using sodium citrate (10 mM, pH 6.5) at 92 °C, followed by blocking of endogenous peroxidase activity with 3% hydrogen peroxide in PBS. Sections were incubated overnight in mouse anti-Pax7 IgG1 (1:100 dilution, Developmental Studies Hybridoma Bank, Iowa City, IA) and rabbit anti-CD9 IgG (1:100 dilution, SA35-08, Invitrogen), followed by incubation in goat anti-mouse biotin-conjugated secondary antibody (dilution 1:1,000, 115-065-205; Jackson ImmunoResearch, West Grove, PA) and Alexa Fluor 647 goat anti-rabbit IgG (1:250 dilution, A32733, Invitrogen) for 1 h at room temperature. Next, sections were incubated with streptavidin-HRP (1:500 dilution, S-911, Invitrogen) and Texas Red-conjugated Wheat Germ Agglutinin (WGA) (1:50 dilution, W21405, Invitrogen) at room temperature for 1 h, before incubation in Tyramide Signal Amplification (TSA) Alexa Fluor 488 (1:500 dilution, B40953, Invitrogen). Sections were mounted with VectaShield fluorescent mounting media with DAPI (H-1200-10, Vector Laboratories).
Images were captured with a Zeiss upright microscope (AxioImager M1, Oberkochen, Germany). To quantify the percentage of nuclei (DAPI+) expressing CD63, MyoVision software was used for automated analysis of nuclear density in cross-sections [39 (link)], and nuclei-expressing CD63 (identified as DAPI+/CD63+ events) were counted manually in a blinded manner by the same assessor for all sections using the Zen Blue software.

Ismaeel A., Van Pelt D.W., Hettinger Z.R., Fu X., Richards C.I., Butterfield T.A., Petrocelli J.J., Vechetti I.J., Confides A.L., Drummond M.J, & Dupont-Versteegden E.E. (2023). Extracellular vesicle distribution and localization in skeletal muscle at rest and following disuse atrophy. Skeletal Muscle, 13, 6.

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Publication 2023

Alexa594 alexa fluor 488 Alexa Fluor 647 anti-IgG Antibodies Antibodies, Anti-Idiotypic Biological Factors Biotin Cardiac Arrest Cell Nucleus Cloning Vectors DAPI DMD protein, human Epitopes Goat Hybridomas IgG1 IgG2B Immunoglobulins Laminin Microscopy Mus Muscle Tissue Novus paraform PAX7 protein, human Peroxidase Peroxides Rabbits Sodium Citrate Streptavidin Technique, Dilution Tritium Wheat Germ Agglutinins

Immunofluorescence of Brain Tissue Sections

15 animals were previously anesthetized by i.p. injection of ketamine (100 mg/Kg) and xylazine (10 mg/Kg). When they were in the no-pain sleep phase, they were intracardially perfused with 4% paraformaldehyde (PFA) diluted in 0.1 M phosphate buffer (PB). After perfusion, brains were removed and stored in 4% PFA at 4 °C overnight (O/N). The next day, the solution was replaced by 4% PFA + 30% sucrose. Coronal sections of 20 μm were obtained by a cryostat (Leica Microsystems, Wetzlar, Germany) and they were kept in a cryoprotectant solution and stored at − 20 °C until use. To perform the experiments, the free-floating technique was used. Briefly, free-floating sections were rinsed in 0.1 M phosphate-buffered saline (PBS) pH 7.35, and after that in PBS-T (PBS 0.1 M, 0.2% Triton X-100). Then they were incubated in a blocking solution (10% fetal bovine serum (FBS), 1% Triton X-100, PBS 0.1 M + 0.2% gelatin) for 1–2 h at room temperature. Later, sections were washed with PBS-T and incubated O/N at 4 °C with the corresponding primary antibody (Table 2). Brain slices were washed with PBS-T and incubated with the corresponding secondary antibody (Table 2) for 2 h at room temperature. Thioflavin-S (ThS) protocol was carried out as previously described [42 (link)]. Finally, sections were treated with 0.1 μg/mL Hoechst (Sigma-Aldrich, St Louis, MO, United States), used for cell nuclei staining, for 8 min in the dark at room temperature and washed with 0.1 M PBS. All reagents, containers and materials exposed to Hoechst were properly handled and processed to avoid any cytotoxic contamination. Ultimately, all the samples were mounted in Superfrost^® microscope slides using Fluoromount medium (EMS) and were left to dry O/N. Image acquisition was obtained using an epifluorescence microscope (BX61 Laboratory Microscope, Melville, NY OlympusAmerica Inc.) and quantified by ImageJ. 5 animals per group were analyzed.Table 2

Primary and secondary antibodies for Immunofluorescence

Protein	Antibody
GFAP	Z0334 (Dako)
IBA1	O19-19741 (Wako)
2nd-ary Alexa Fluor 488 (Goat-AntiMouse)	A11001 (Life Technologies)
2nd-ary Alexa Fluor 594 (Goat-Anti Rabbit)	A11080 (Life Technologies)

Espinosa-Jiménez T., Cano A., Sánchez-López E., Olloquequi J., Folch J., Bulló M., Verdaguer E., Auladell C., Pont C., Muñoz-Torrero D., Parcerisas A., Camins A, & Ettcheto M. (2023). A novel rhein-huprine hybrid ameliorates disease-modifying properties in preclinical mice model of Alzheimer’s disease exacerbated with high fat diet. Cell & Bioscience, 13, 52.

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Publication 2023

Alexa594 alexa fluor 488 Animals Antibodies Brain Cell Nucleus Cryoprotective Agents Fetal Bovine Serum Gelatins Goat Immunoglobulins Ketamine Microscopy Pain paraform Perfusion Phosphates Rabbits Saline Solution Sleep Stages Sucrose thioflavin S Triton X-100 Xylazine

Intracellular Visualization Using Two-Photon Microscopy

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Publication 2023

Acceleration Acetone Alexa594 Anti-Antibodies Buffers cadherin 5 Cells DAPI Dehydration F-Actin fluorescein isothiocyanate-phalloidin Glutaral Gold IGF II Immunoglobulins Microscopy Palladium Phalloidine Phosphates Polaron Proteins Protoplasm Reconstructive Surgical Procedures Sapphire Technique, Dilution tetramethylrhodamine isothiocyanate Triton X-100 Vacuum

Immunofluorescent Characterization of iPSC-Derived Cardiomyocytes

IPSc-derived cardiomyocytes were cultured on glass slides coated with Synthemax II-SC (Corning) in RPMI-1640/B-27 with Y27632 (5 μM) media for 2 days. Cells were fixed with 4% formaldehyde for 15 min at room temperature. Fixed cells were blocked in antibody buffer (5% BSA, 0.1% Tween-20 in PBS) for 1 h at room temperature. Following blocking, cells were incubated overnight at 4 °C with cardiac troponin-T antibody (abcam, ab45932, 1:200), α-Actinin antibody (Sigma, A7811, 1:800), and/or Connexin 43 (Cell Signaling Technology, 1:100) in antibody buffer. After the overnight incubation, cells were washed three times in antibody buffer. Following washing, cells were incubated with Alexa-Fluor conjugated secondary antibodies (Thermo Fisher Scientific, Alexa 488 Donkey anti-Rabbit IgG and Alexa 594 Goat anti-Ms IgG1) at a 1:1,000 dilution in antibody buffer for 1 h at room temperature. Nuclei were stained by DAPI at 1 μg/ml and Wheat Germ Agglutinin (Thermo Fisher Scientific, W21404) was used to stain cell membrane for 10 min at room temperature in antibody buffer. Following washing in PBS to remove unbound complexes, sarcomeres were analyzed using a Zeiss LSM510 confocal microscope. Images were processed with Zeiss software (Axiovision Rel4.8 and Zen Blue). Circularity measurements were made by comparing cardiomyocyte length to width and were expressed as a circularity index whereby circularity index = width/length^{15 (link)}. Sarcomere distance measurements were made with ImageJ^{15 (link)}. All measurements were made with a double-blinding method.

Hsueh Y.C., Pratt R.E., Dzau V.J, & Hodgkinson C.P. (2023). Novel method of differentiating human induced pluripotent stem cells to mature cardiomyocytes via Sfrp2. Scientific Reports, 13, 3920.

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Publication 2023

Actinin Alexa594 anti-IgG Antibodies Buffers Cardiac Arrest Cell Nucleus DAPI Equus asinus Formaldehyde GJA1 protein, human Goat Heart IgG1 Immunoglobulins Induced Pluripotent Stem Cells Microscopy, Confocal Myocytes, Cardiac Plasma Membrane Rabbits Sarcomeres Stains Technique, Dilution Troponin T Tween 20 Wheat Germ Agglutinins Y 27632

Quantifying NPC Proliferation with EdU

Here, 3*10⁴ cells of NPCs were seeded in 24-well plates. Cells were treated with IL-1β (10 ng/mL) with or without DHJSD (300 μg/mL) for 24 h and then tested following the manufacturer’s instructions (BeyoClick™ EdU Cell Proliferation Kit with Alexa Fluor 594). DAPI was used to stain the cell nucleus before observation. Images were captured under a microscope (Olympus).

Liu W., Zhao X, & Wu X. (2023). Duhuo Jisheng Decoction suppresses apoptosis and mitochondrial dysfunction in human nucleus pulposus cells by miR-494/SIRT3/mitophagy signal axis. Journal of Orthopaedic Surgery and Research, 18, 177.

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Publication 2023

Alexa594 Cell Nucleus Cell Proliferation DAPI Interleukin-1 beta Microscopy Stains

Top products related to «Alexa594»

Alexa fluor 594 by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Germany, Japan, Canada, France, China, Denmark, Netherlands, Italy

Alexa Fluor 594 is a fluorescent dye developed by Thermo Fisher Scientific. It is designed to emit light in the red-orange region of the visible spectrum when excited with appropriate wavelengths of light. The dye can be used in various biological and biochemical applications that require a fluorescent label.

Alexa fluor 488 by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Germany, Japan, France, Italy, Canada, China, Spain, Switzerland, Denmark, Australia, Hungary, Belgium, Ireland, Israel, Netherlands, Moldova, Republic of, India, Austria, Czechia, Poland

Alexa Fluor 488 is a fluorescent dye used in various biotechnological applications. It has an excitation maximum at 495 nm and an emission maximum at 519 nm, producing a green fluorescent signal. Alexa Fluor 488 is known for its brightness, photostability, and pH-insensitivity, making it a popular choice for labeling biomolecules in biological research.

4 6 diamidino 2 phenylindole (dapi) by Thermo Fisher Scientific

Sourced in United States, Germany, United Kingdom, Japan, China, Canada, Italy, Australia, France, Switzerland, Spain, Belgium, Denmark, Panama, Poland, Singapore, Austria, Morocco, Netherlands, Sweden, Argentina, India, Finland, Pakistan, Cameroon, New Zealand

DAPI is a fluorescent dye used in microscopy and flow cytometry to stain cell nuclei. It binds strongly to the minor groove of double-stranded DNA, emitting blue fluorescence when excited by ultraviolet light.

4 6 diamidino 2 phenylindole (dapi) by Merck Group

Sourced in United States, Germany, Japan, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, Canada, Switzerland, Spain, Australia, Denmark, India, Poland, Israel, Belgium, Sweden, Ireland, Netherlands, Panama, Brazil, Portugal, Czechia, Puerto Rico, Austria, Hong Kong, Singapore

DAPI is a fluorescent dye that binds strongly to adenine-thymine (A-T) rich regions in DNA. It is commonly used as a nuclear counterstain in fluorescence microscopy to visualize and locate cell nuclei.

Alexa 594 by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Canada, Switzerland, Japan, Germany, France, Spain

Alexa Fluor 594 is a fluorescent dye produced by Thermo Fisher Scientific. It is designed for use in a variety of biological applications, including flow cytometry, immunohistochemistry, and fluorescence microscopy. The dye has an excitation maximum at 590 nm and an emission maximum at 617 nm, making it compatible with common fluorescence detection systems.

Triton x 100 by Merck Group

Sourced in United States, Germany, United Kingdom, Italy, China, Japan, Canada, France, Sao Tome and Principe, Switzerland, Macao, Poland, Spain, Australia, India, Belgium, Israel, Sweden, Ireland, Denmark, Brazil, Portugal, Panama, Netherlands, Hungary, Czechia, Austria, Norway, Slovakia, Singapore, Argentina, Mexico, Senegal

Triton X-100 is a non-ionic surfactant commonly used in various laboratory applications. It functions as a detergent and solubilizing agent, facilitating the solubilization and extraction of proteins and other biomolecules from biological samples.

Bovine serum albumin (bsa) by Merck Group

Sourced in United States, Germany, United Kingdom, China, Italy, Japan, France, Sao Tome and Principe, Canada, Macao, Spain, Switzerland, Australia, India, Israel, Belgium, Poland, Sweden, Denmark, Ireland, Hungary, Netherlands, Czechia, Brazil, Austria, Singapore, Portugal, Panama, Chile, Senegal, Morocco, Slovenia, New Zealand, Finland, Thailand, Uruguay, Argentina, Saudi Arabia, Romania, Greece, Mexico

Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.

Alexa 488 by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Germany, France, Japan, Switzerland, Canada, Panama, China, Italy, Denmark, Spain

Alexa 488 is a fluorescent dye used in various biological applications. It is a brighly fluorescent, green-emitting dye with excitation and emission maxima at 495 and 519 nm, respectively. Alexa 488 can be conjugated to biomolecules such as proteins, antibodies, or nucleic acids to enable their detection and visualization.

Hoechst 33342 by Thermo Fisher Scientific

Sourced in United States, Germany, United Kingdom, Japan, China, France, Canada, Spain, Belgium, Italy, Australia, Austria, Denmark, Netherlands, Switzerland, Ireland, New Zealand, Portugal, Brazil, Argentina, Singapore, Poland, Ukraine, Macao, Thailand, Finland, Lithuania, Sweden

Hoechst 33342 is a fluorescent dye that binds to DNA. It is commonly used in various applications, such as cell staining and flow cytometry, to identify and analyze cell populations.

Alexa fluor 594 goat anti rabbit igg by Thermo Fisher Scientific

Sourced in United States, Japan, China, United Kingdom, Germany, Italy, Canada

Alexa Fluor 594 goat anti-rabbit IgG is a fluorescent secondary antibody used for detection and visualization in immunoassays and other applications. It is conjugated to the Alexa Fluor 594 dye, which has an excitation maximum at 590 nm and an emission maximum at 617 nm.

What are the key features and benefits of Alexa Fluor 594?

Alexa Fluor 594 is a highly fluorescent and photostable dye that excites at 590 nm and emits at 617 nm. It is commonly used for labeling antibodies, proteins, and other biomolecules in a variety of assays like immunohistochemistry, flow cytometry, and live-cell imaging. Alexa Fluor 594 is known for its bright signal and compatibility with standard red fluorescent protein and rhodamine filter sets, making it a popular choice for researchers studying cellular processes, protein localization, and molecular interactions.

How can PubCompare.ai help with the use of Alexa Fluor 594?

PubCompare.ai's AI-driven platform can assist researchers in optimizing their use of Alexa Fluor 594. The tool allows you to efficiently screen protocol literature to identify the most effective procedures for your specific research goals involving Alexa Fluor 594. The platform's analysis can highlight key differences in protocol effectiveness, enabling you to choose the best option to ensure reproducibility and accuracy in your experiments.

Are there any variations or types of Alexa Fluor 594 that researchers should be aware of?

Yes, there are several variations of Alexa Fluor 594 that researchers may encounter. For example, there are Alexa Fluor 594 succinimidyl ester and Alexa Fluor 594 maleimide versions, which are designed for different types of biomolecule conjugation. Additionally, Alexa Fluor 594 is part of the broader Alexa Fluor dye series, which includes other fluorescent dyes with different excitation and emission profiles. Researchers should carefully consider the specific properties and compatibility of the Alexa Fluor 594 variant they choose to ensure optimal results in their experiments.

What are some common applications of Alexa Fluor 594 in biological research?

Alexa Fluor 594 is a versatile dye that is widely used in a variety of biological research applications. It is frequently conjugated to antibodies for immunohistochemistry and flow cytometry to visualize and detect specific proteins or cell populations. Alexa Fluor 594 is also used to label proteins, nucleic acids, and other biomolecules for live-cell imaging, allowing researchers to study cellular processes and molecular interactions in real-time. Additionally, Alexa Fluor 594 can be employed in ELISA, Western blotting, and other assays that require sensitive and specific detection of target analytes.

How can PubCompare.ai help researchers optimize their use of Alexa Fluor 594?

PubCompare.ai's AI-driven platform can be a valuable tool for researchers using Alexa Fluor 594 in their experiments. The tool allows you to efficiently screen protocol literature, including journal articles, pre-prints, and patents, to identify the most effective procedures for labeling, detecting, and visualizing Alexa Fluor 594 in your specific research context. The platform's analysis can highlight key differences in protocol effectiveness, enabling you to choose the best option to ensure reproducibility and accuracy in your experiments. By leveraging PubCompare.ai, you can streamline your research and improve the efficiency of your Alexa Fluor 594-based studies.

More about "Alexa594"

Alexa Fluor 594 is a popular fluorescent dye used extensively in biological research.
It is part of the Alexa Fluor dye series, renowned for their bright fluorescence and excellent photostability.
With an excitation maximum at 590 nm and an emission maximum at 617 nm, Alexa Fluor 594 is compatible with common red fluorescent protein and rhodamine filter sets.
This versatile dye is frequently conjugated to antibodies, proteins, or other biomolecules to enable visualization and detection in a variety of assays, including immunohistochemistry, flow cytometry, and live-cell imaging.
Its bright signal and photostability make it a popular choice for fluorescent labeling and detection in the life sciences.
In addition to Alexa Fluor 594, researchers may also utilize other fluorescent dyes like Alexa Fluor 488, DAPI, Alexa 594, and Hoechst 33342 for multicolor experiments.
Detergents such as Triton X-100 and blocking agents like bovine serum albumin (BSA) are often used in conjunction with these fluorescent probes to optimize staining and imaging protocols.
Optimizing research protocols and improving reproducibility is crucial for scientific progress.
PubCompare.ai's AI-driven platform can help researchers easily locate and compare protocols from literature, pre-prints, and patents, ensuring they identify the best protocols and products for their needs.
By streamlining the research process and providing advanced tools and analytics, PubCompare.ai can help researchers improve their efficiency and the quality of their work.