Sequences of A. palmeri EPSPS gene (GenBank accession no. JX564536) were used to develop the PCR primers for cloning of the EPSPS gene. The PCR product was cloned in 2.1-TOPO TA vector (Invitrogen), and the clone was labeled with digoxigenin-11-deoxyuridine triphosphate (Roche Diagnostics) using a standard nick translation reaction. The clone, maize 5S rDNA (54 (link)), was labeled with biotin-16-dUTP (Roche). The BAC clones were labeled with either biotin-16-dUTP or digoxigenin-11-dUTP using a nick translation reaction. Biotin- and digoxigenin-labeled probes were detected with Alexa Fluor 488 streptavidin antibody (Invitrogen) and rhodamine-conjugated antidigoxigenin antibody (Roche), respectively.
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Digoxigenin-11-deoxyuridine triphosphate
Digoxigenin-11-deoxyuridine triphosphate
Digoxigenin-11-deoxyuridine triphosphate is a synthetic nucleotide analog used in molecular biology techniques, such as in situ hybridization and immunohistochemistry.
It allows for the sensitive and specific detection of target DNA or RNA sequences by incorporating the digoxigenin-labeled deoxyuridine triphosphate into newly synthesized nucleic acid strands.
This versatile label can be detected using anti-digoxigenin antibodies, enabling visualization and quantification of the labeled targets.
Researchers can utilize PubComapre.ai's AI-driven platform to explore optimized protocols and methods for using Digoxigenin-11-deoxyuridine triphosphate, locating relevant literature, preprints, and patents, and identifying the best techniques and products through intelligent comparisons.
It allows for the sensitive and specific detection of target DNA or RNA sequences by incorporating the digoxigenin-labeled deoxyuridine triphosphate into newly synthesized nucleic acid strands.
This versatile label can be detected using anti-digoxigenin antibodies, enabling visualization and quantification of the labeled targets.
Researchers can utilize PubComapre.ai's AI-driven platform to explore optimized protocols and methods for using Digoxigenin-11-deoxyuridine triphosphate, locating relevant literature, preprints, and patents, and identifying the best techniques and products through intelligent comparisons.
Most cited protocols related to «Digoxigenin-11-deoxyuridine triphosphate»
alexa fluor 488
Biotin
biotin-16-dUTP
Clone Cells
Cloning Vectors
Diagnosis
Digoxigenin
digoxigenin-11-deoxyuridine triphosphate
Excitatory Postsynaptic Potentials
Genes
Immunoglobulins
Maize
Oligonucleotide Primers
Recombinant DNA
Rhodamine
Streptavidin
trioctyl phosphine oxide
The cells/sections on coverslips or the sections on grids were washed with distilled water before reverse transcription. The following components were used in the reaction mixture (RM) during testing: 1 × AMV reverse transcriptase buffer (Promega, 5 × AMV reverse transcriptase buffer: 250 mM Tris–HCl, 250 mM KCl, 50 mM MgCl2, 2.5 mM spermidine, 50 mM DTT), 0.2 U/µl AMV reverse transcriptase (Promega), 0.4 U/µl RNasin (Promega), 0.25 mM dATP, dGTP, dCTP and dTTP (Promega), 0.25 mM 5-bromo-2′-deoxyuridine triphosphate (BrdUTP, Sigma Aldrich), 0.05 mM biotin-16-2′-deoxyuridine-5′-triphosphate (biotin-dUTP, Roche), 0.05 mM digoxigenin-11-2′-deoxyuridine-5′-triphosphate (digoxigenin-dUTP, Roche), 0.05 mM Alexa Fluor® 555-aha-2′-deoxyuridine-5′-triphosphate (alexa-dUTP, Invitrogen), 0.05 mM ChromaTide® fluorescein-12-2′-deoxyuridine-5′-triphosphate (fluorescein-dUTP, Invitrogen) and 0.01 µg/µl oligonucleotides. The following oligonucleotides were used: oligonucleotides consisting of 15, 20 or 25 deoxythymidines (oligo dT15, Promega; oligo dT20, oligo dT25, Generi Biotech), oligonucleotides consisting of 15 deoxyadenines (oligo dA15, Generi Biotech) or a random hexanucleotide (Promega). The coverslips/grids were incubated on the 20 µl/10 µl drops of the RM in the moisture chamber for 1 h at 42°C and washed in 1 × PBS. Coverslips with cells labelled with alexa–dUTP or fluorescein-dUTP exclusively were washed in 1 × PBS and distilled water and mounted in Mowiol. The coverslips/grids with fluorescently non-labelled nucleotide analogues were incubated for 1 h with the primary antibody and after washing in PBS they were incubated for 1 h with the secondary antibody. The following primary antibodies were used in the comparative studies focused on the detection of cDNA tagged by bromodeoxyuridine (BrdU) or biotin-dUTP: a rabbit anti-biotin antibody (Enzo Lifesciences) diluted 1:100 in 1× PBS and a mouse anti-BrdU antibody (Roche) diluted 1:20 in 1× PBS. Digoxigenin-dUTP was detected by a mouse anti-digoxigenin antibody (Roche) diluted 1:100 in 1× PBS. For a comparison of the different antibodies against the BrdU, a rat anti-BrdU antibody (Abcam) diluted 1:100 in 1× PBS and a mouse anti-BrdU antibody (Becton-Dickinson) diluted 1:4 in 1× PBS were used in addition to the mouse anti-BrdU antibody purchased from Roche. For the detection of the splicing factor SC35, the mouse anti-SC35 antibody (Abcam) diluted 1:500 in 1× PBS was used. For LM, we used the anti-rabbit, anti-rat or anti-mouse secondary antibody conjugated with Cy3 or FITC fluorochrome. All of the secondary antibodies were diluted 1:100 in 1× PBS and were purchased from Jackson Immunoresearch. In the comparative studies focused on the detection of cDNA tagged by BrdU or biotin-dUTP and the comparision of different antibodies against BrdU, secondary antibodies conjugated with Cy3 were used exclusively. The chosen secondary antibodies (cat. number 115-165-146 and 111-165-144) provided a similar strength of the replication signal after the hypotonic introduction (10 (link)) of biotin-dUTP and BrdUTP into cells. For EM, we used an anti-mouse antibody conjugated with 10 nm of gold adduct diluted 1:100 in 1× PBS (Aurion). After incubation with the secondary antibody, the cells on coverslips were washed on drops of 1× PBS, then on drops of distilled water and then mounted in Mowiol. The sections on coverslips were washed on drops of 1× PBS, drops of distilled water, stained with DAPI for 20 min, washed in distilled water and mounted in Mowiol. The sections on grids were washed in 1× PBS, then in distilled water and subsequently incubated on drops of 3% uranyl acetate for 45 min. After that, the grids were washed in distilled water and dried.
The following components were used in the RM during the control experiments with DNA polymerase I, E. coli (Fermentas): 1 × DNA polymerase I, E. coli buffer (Fermentas, 10x polymerase I buffer: 500 mM Tris–HCl, 100 mM MgCl2, 10 mM DTT), 0.2 U/µl DNA polymerase I, E. coli, 0.25 mM dATP, dGTP, dCTP and dTTP (Promega), 0.25 mM BrdUTP and 0.05 mM biotin-dUTP.
In the other control experiments, the cells/sections were treated by DNase I or RNase A. We used DNase I (RNase-free, Fermentas, 0.1 U/µl in 10 mM Tris–Cl, pH 7.5, 2.5 mM MgCl2 and 0.1 mM CaCl2) or RNase A (DNase and protease free, Fermentas, 5 µg/ml in 10 mM Tris-Cl, pH 7.4).
The following components were used in the RM during the control experiments with DNA polymerase I, E. coli (Fermentas): 1 × DNA polymerase I, E. coli buffer (Fermentas, 10x polymerase I buffer: 500 mM Tris–HCl, 100 mM MgCl2, 10 mM DTT), 0.2 U/µl DNA polymerase I, E. coli, 0.25 mM dATP, dGTP, dCTP and dTTP (Promega), 0.25 mM BrdUTP and 0.05 mM biotin-dUTP.
In the other control experiments, the cells/sections were treated by DNase I or RNase A. We used DNase I (RNase-free, Fermentas, 0.1 U/µl in 10 mM Tris–Cl, pH 7.5, 2.5 mM MgCl2 and 0.1 mM CaCl2) or RNase A (DNase and protease free, Fermentas, 5 µg/ml in 10 mM Tris-Cl, pH 7.4).
To create an rDNA specific probe, the pNOY373 plasmid, a derivative of the high copy number plasmid YEp351 carrying rDNA with a promoter starting from –206 with a XhoI–NotI flanked enhancer, LEU2, 2µ, amp, was used. The pNOY373 plasmid was kindly provided by Prof. Masayasu Nomura (University of California, USA) (Wai et al. 2000 (link)). pNOY373 DNA containing the 18S rRNA coding region [1 µg] was labelled with digoxigenin-11-deoxyuridine 5′-triphosphate (dUTP) using the DIG Nick Translation Mix (Roche) according to manufacturer’s instruction. After PFGE separation (CHEF-DR®III Pulsed Field Electrophoresis System, Biorad), yeast chromosomes were transferred onto a nylon membrane (Roche) by capillary transfer. Then, the membrane was hybridised to a digoxigenin (DIG)-labelled rDNA-specific probe and rDNA was detected with an alkaline phosphatase-conjugated anti-DIG antibody. The chemiluminescence signal was detected with the substrate for alkaline phosphatase (CDP-Star) and the G:BOX imaging system (Syngene, Cambridge, UK). rDNA and ERCs were quantified using GelQuantNET software (http://biochemlabsolutions.com/GelQuantNET.html ) using the background correction option. The amount of genomic rDNA and multimer ERCs were calculated per mean amount of DNA.
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Alkaline Phosphatase
Antibodies, Anti-Idiotypic
Capillaries
Chemiluminescence
Chromosomes
Digoxigenin
digoxigenin-11-deoxyuridine triphosphate
Electrophoresis
Electrophoresis, Gel, Pulsed-Field
Genome
Nylons
Plasmids
Recombinant DNA
RNA, Ribosomal, 18S
Tissue, Membrane
Yeast, Dried
Whole genomic DNAs (gDNAs) from P. ridibundus and P. lessonae were extracted from muscle tissue using the conventional phenol-chloroform-isoamylalcohol method [13 (link)]. Probes prepared from both parental species were differentially labelled either with biotin-16-dUTP (2’-Deoxyuridine, 5’-Triphosphate, Roche, Mannheim, Germany) or digoxigenin-11-dUTP (Roche) using Nick Translation Mix (Abbott Molecular, Illinois, USA or Roche Diagnostics, Mannheim, Germany). For each slide, 1 μg of P. ridibundus gDNA, 1 μg of P. lessonae gDNA and 50 μg of sonicated salmon sperm DNA (Sigma-Aldrich) were added and the resulting probe was precipitated in 96 % ethanol, washed in 70 % ethanol, air-dried and re-dissolved in 25 μl of hybridization buffer (50 % formamide, 10 % dextran sulphate, 2× SSC (Standard saline buffer), 0.04 M NaPO4 (Sodium Phosphate) buffer, 0.1 % SDS, Denhardt’s reagent, see [29 ]). In some experiments, the final probe also included 15–30 μg of unlabelled species-specific competitive DNA prepared from P. esculentus gDNA using a Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Buckinghamshire, UK), followed by sonication of the amplified product (40 cycles, 10 pulses, 100 % power) to approximate fragment size of 100–200 bp using the ultrasonic homogenizer Sonopuls HD 2070 (Bandelin Electric, Berlin, Germany).
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Acid Hybridizations, Nucleic
biotin-16-dUTP
BP 100
Buffers
Chloroform
Deoxyuridine
Diagnosis
digoxigenin-11-deoxyuridine triphosphate
Electricity
Ethanol
formamide
Genome
Muscle Tissue
Parent
Phenol
Pulses
Saline Solution
Salmo salar
sodium phosphate
Sperm
Sulfate, Dextran
triphosphate
Ultrasonics
Male and female genomic DNA were labelled with biotin- deoxyuridine triphosphate (dUTP) and digoxigenin-dUTP, respectively, using a Nick Translation Kit (Abbott Laboratories, Lake Bluff, IL, USA). From each sample, 1 μg of male and 1 μg of female labelled genomic DNA was co-precipitated overnight with 5 μL salmon sperm DNA (10 mg/mL, Sigma), 10 μL of 3M sodium acetate and 2.5× volume of ethanol. After precipitation, the dry pellets were resuspended in 22 μL hybridization buffer (50% formamide, 2× SSC, 10% SDS, 10% dextran sulfate, 1× Denhardt’s buffer, pH 7), denatured at 75 °C for 10 min and then chilled on ice for 10 min prior to hybridization. At the same time, the metaphase slides were treated with RNase and pepsin, fixed with 4% formaldehyde, dehydrated through a 70, 85 and 100% ethanol series, denatured in 70% formamide/2× SSC at 75 °C for 3 min and dehydrated again. For the next step, 11 μL of the probe (concentration approx. 500 ng of labeled DNA) was applied on the slide per drop of chromosomal suspension and incubated at 37 °C for 48 h. Post-hybridization washes were performed in 50% formamide/2× SSC at 42 °C and in 2× SSC. Each slide was incubated with 100 μL of 4× SSC/5% blocking reagent (Roche, Basel, Switzerland) at 37 °C for 30 min and then with 100 μL detection solution 4× SSC/5% blocking reagent including 2 μL of avidin-FITC (Vector Laboratories, Burlingame, CA, USA) and 10 μL of anti-digoxigenin-rhodamine (Roche, Basel, Switzerland) at 37 °C for 30 min. The slides were subsequently washed in 4× SSC/0.05% Tween 20, dehydrated through an ethanol series and air dried. Finally, the slides were mounted with Fluoroshield antifade medium containing DAPI (Sigma-Aldrich, St. Louis, MO, USA). For our detailed protocol see [27 (link)].
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Acid Hybridizations, Nucleic
Biotin
Buffers
Chromosomes
Cloning Vectors
DAPI
deoxyuridine triphosphate
Digoxigenin
Ethanol
Females
fluorescein isothiocyante avidin
Fluoroshield
Formaldehyde
formamide
Genome
Males
Metaphase
Pellets, Drug
Pepsin A
Rhodamine
Ribonuclease, Pancreatic
Salmo salar
Sodium Acetate
Sperm
Sulfate, Dextran
Tween 20
Most recents protocols related to «Digoxigenin-11-deoxyuridine triphosphate»
In order to generate probes for FISH, genomic DNA (gDNA) from hexaploid C. gibelio was used as a template for amplification of the U1 and U2 snDNA regions, and H3 histone. DNA was extracted from adult fish tissues using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. Primers used for amplification are listed in Table 2 . The annealing temperature was 54 °C and the elongation step 30 s for all polymerase chain reactions (PCR); other conditions for PCR amplification with PPP Master Mix (Top-Bio, Prague, Czech Republic) followed the manufacturer’s recommendations. PCR amplification of the U1, U2, and H3 genes consistently resulted in 112, 140, and 375 bp long fragments, respectively. The search using the blastn algorithm confirmed the locus- and species-specificity of each amplicon: 98.98% identity with the U1 DNA sequence of C. gibelio (accession number XR_008182931.1), 97.10% identity with the U2 DNA sequence of C. gibelio (accession number XR_008154662.1), and 97.53% identity with H3 DNA of C. gibelio (accession number XM_052561945.1). Labeling PCR was performed as described in Knytl and Fornaini (2021) (link). Digoxigenin-11-deoxyuridine triphosphate (dUTP) (Jena Bioscience, Jena, Germany) was used for U2 and H3 labeling, and biotin-16-dUTP (Jena Bioscience) was used for U1 labeling. Carassius gibelio U1 and U2 snDNA and H3 probes were then hybridized to chromosome spreads of C. carassius and C. auratus and tetraploid and hexaploid C. gibelio. The procedures for hybridization mixture preparation, denaturation, and subsequent overnight hybridization were previously described for rDNA FISH (Knytl et al. 2023 (link)). Post-hybridization stringency washing and blocking reactions were performed as described for painting FISH in Krylov et al. (2010) (link). Probe signal was visualized following Knytl et al. (2017) (link).
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A TUNEL staining analysis kit was used according to the manufacturer's instructions. Briefly, samples were treated with 3% H2O2 and then digested with proteinase K (20 µg/ml) at 25°C. After approximately 10 min, the samples were cultured with the labeled buffer (1:18) at 37°C. After approximately 2 h, the samples were incubated with biotinylated anti‐digoxin antibody (1:100) for about 30 min. Streptavidin‐biotin peroxidase was used to detect luciferin. Next, 3,3'‐diaminobenzidine (DAB) was used to dye the samples. The 3'‐OH terminal DNA fragment was labeled using terminal deoxynucleotidyl transferase with digoxigenin‐11‐deoxyuridine triphosphate (DIG dUTP) to measure cell death. Dead cells were stained brown, whereas healthy cells were colored. The death index refers to the ratio of brown‐stained nuclei to total nuclei. For each sample, nuclei from 10 regions were analyzed.
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Antibodies, Anti-Idiotypic
Biotin
Buffers
Cell Death
Cell Nucleus
Cells
digoxigenin-11-deoxyuridine triphosphate
Digoxin
DNA Nucleotidylexotransferase
Endopeptidase K
In Situ Nick-End Labeling
Luciferins
Peroxidase
Peroxide, Hydrogen
Streptavidin
For Southern blot analysis, 20 µg of plant genomic DNA was digested with XbaI (Promega, USA), resolved by electrophoresis in 1% agarose gel, and transferred onto a nylon membrane Hybond N+ (Roche, Germany) (Hoisington et al. 1994 ). The membrane was pre-hybridized for 3 h in hybridization buffer containing 0.2% sodium dodecyl sulfate (SDS), 0.1% sodium N-lauroyl sarcosinate, 5x SSC solution (0.75M NaCl, 0.075M sodium citrate), 1% blocker (Roche, Germany), and denatured herring sperm DNA (Promega, USA). A 354-bp probe labeled with digoxigenin-11-deoxyuridine-5′triphosphate (Dig-dUTP; Roche, Germany) was prepared by PCR, using ipt354-Fw and ipt354-Rv oligonucleotides (T). The membrane was hybridized overnight at 55°C. The hybridized membrane was washed three times with 2X, 0.2X, and 0.1X of SSC (pH 7.2), respectively, and 0.1% m/v of SDS at 65°C. The chemiluminescent signals were detected on Amersham Hyper lmTM ECL (Ge, EEUU) and the UVP SYNGENE® system.
To detect sgRNAs complexed with Cas9, 1 μl of Cas9 (1 μM) (Alt-R S.p. Cas9 Nuclease V3, IDT) and 1 μl of synthetic sgRNAs (3 μM, 1 μM or 0.3 μM; IDT) were mixed with 8 μl of distilled water (total reaction volume of 10 μl) and reacted on ice for 30 min. Samples were loaded onto Bullet PAGE One Precast gels (6%) (Nacalai Tesque) in Tris-borate-ethylenediaminetetraacetic acid (Tris-Borate-EDTA) buffer. RNA was transferred to a Hybond N+ membrane (GE Healthcare) and cross-linked using CX-2000 (Analytik Jena). An sgRNA tracer probe was labelled with an alkali-labile digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) using a PCR DIG Probe Synthesis kit (Roche); DNA fragments were amplified using PCR and primers (Supplementary Table 3 ). After hybridization, specific bands were visualized with the CDP-Star reagent (Roche) using a luminescent image analyser (LAS-3000, FUJIFILM).
To detect DNA fragments complexed with sgRNA-dCas9, we mixed 1 μl of dCas9 (1 μM) (Alt-R S.p. dCas9 Nuclease V3, IDT) and 1 μl of synthetic sgRNAs (1 μM; IDT) with distilled water for a final reaction volume of 10 μl, then reacted the mixture at room temperature for 10 min. After the reaction, the RNP complex was mixed with 100 ng of DNA fragment and 1 μl of 10× Cas9 reaction buffer (1 M HEPES, 3 M NaCl, 1 M MgCl2 and 250 mM EDTA (pH 6.5)), then reacted at room temperature for 10 min. The resulting 10 μl samples were loaded onto 2% agarose gels in Tris-acetate-EDTA buffer; DNA bands were detected by staining with ethidium bromide. The target DNA fragment (647 bp) was prepared by PCR amplification from a Tbx3-P2A1-Venus KI plasmid using primers (Supplementary Table3 ).
To detect DNA fragments complexed with sgRNA-dCas9, we mixed 1 μl of dCas9 (1 μM) (Alt-R S.p. dCas9 Nuclease V3, IDT) and 1 μl of synthetic sgRNAs (1 μM; IDT) with distilled water for a final reaction volume of 10 μl, then reacted the mixture at room temperature for 10 min. After the reaction, the RNP complex was mixed with 100 ng of DNA fragment and 1 μl of 10× Cas9 reaction buffer (1 M HEPES, 3 M NaCl, 1 M MgCl2 and 250 mM EDTA (pH 6.5)), then reacted at room temperature for 10 min. The resulting 10 μl samples were loaded onto 2% agarose gels in Tris-acetate-EDTA buffer; DNA bands were detected by staining with ethidium bromide. The target DNA fragment (647 bp) was prepared by PCR amplification from a Tbx3-P2A1-Venus KI plasmid using primers (Supplementary Table
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Alkalies
Anabolism
Borates
Buffers
Crossbreeding
Digoxigenin
digoxigenin-11-deoxyuridine triphosphate
Edetic Acid
Ethidium Bromide
Gels
HEPES
Luminescence
Magnesium Chloride
Oligonucleotide Primers
Plasmids
Sepharose
Sodium Chloride
Tissue, Membrane
tris-acetate-EDTA buffer
Tris-borate-EDTA buffer
Tromethamine
Electrophoretic mobility shift assays (EMSAs) were used to assess the regulation of Oct-4/Sox2 by CDX2. Probes with the CDX2-binding site sequence (see Table 1 ) were labeled with Digoxigenin-11-deoxyuridine 5-triphosphate (DIG-ddUTP). Cell protein-DNA complexes were examined with and without competitors or the anti-CDX2 antibody. After electrophoretic separation, the complexes were probed auto-radiographically using a 2nd-generation DIG Gel Shift Kit (Roche, Mannheim, Germany).
2',3'-dideoxyuridine-5'-triphosphate
Antibodies, Anti-Idiotypic
Binding Sites
Cells
digoxigenin-11-deoxyuridine triphosphate
Electrophoresis
Electrophoretic Mobility Shift Assay
HSP40 Heat-Shock Proteins
POU5F1 protein, human
SOX2 Transcription Factor
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The Nylon membrane is a laboratory equipment used for filtration and separation processes. It is a semi-porous material made of nylon that allows the passage of certain substances while retaining others, depending on the membrane's pore size. The Nylon membrane is designed to provide efficient and reliable performance in various laboratory applications.
Digoxigenin-11-deoxy-uridine-triphosphate is a modified nucleotide used in molecular biology techniques. It can be incorporated into nucleic acid probes for the detection and localization of specific DNA or RNA sequences.
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4-Thio-UTP is a modified nucleotide used in various biochemical and molecular biology applications. It serves as a substrate for enzymatic reactions and can be incorporated into nucleic acids, such as RNA. The sulfur atom at the 4-position of the uracil base is the key structural feature of this compound.
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More about "Digoxigenin-11-deoxyuridine triphosphate"
Digoxigenin-11-deoxyuridine triphosphate (Dig-11-dUTP) is a synthetic nucleotide analog widely used in molecular biology techniques such as in situ hybridization and immunohistochemistry.
This versatile label allows for the sensitive and specific detection of target DNA or RNA sequences by incorporating the digoxigenin-labeled deoxyuridine triphosphate into newly synthesized nucleic acid strands.
The Dig-11-dUTP can be detected using anti-digoxigenin antibodies, enabling visualization and quantification of the labeled targets.
Researchers can utilize PubCompare.ai's AI-driven platform to explore optimized protocols and methods for using Dig-11-dUTP, locating relevant literature, preprints, and patents, and identifying the best techniques and products through intelligent comparisons.
Nylon membranes are commonly used in conjunction with Dig-11-dUTP for techniques like Southern and Northern blotting, where the target nucleic acids are immobilized on the membrane and probed with the labeled nucleotide.
The Illustra GenomiPhi V2 DNA Amplification Kit can be used to amplify DNA samples, which may then be labeled with Dig-11-dUTP for in situ hybridization or other applications.
The In Situ Cell Death Detection Kit, which utilizes terminal deoxynucleotidyl transferase (TdT) to incorporate Dig-11-dUTP into DNA breaks, can be used to detect apoptotic cells.
The CHEF-DR®III Pulsed Field Electrophoresis System is another useful tool for separating large DNA fragments, which can then be analyzed using Dig-11-dUTP labeling.
Rhodamine-conjugated anti-digoxigenin antibodies can be used to visualize the Dig-11-dUTP-labeled targets, while 4-Thio-UTP is a related nucleotide analog that can also be used for labeling.
The QIAquick Gel Extraction Kit can be employed to purify DNA fragments labeled with Dig-11-dUTP, and the X-Omat processor is a common instrument used for developing autoradiographic films or chemiluminescent signals generated by Dig-11-dUTP detection.
Overall, Dig-11-dUTP is a versatile and widely used tool in molecular biology research, and PubCompare.ai can help researchers optimize their protocols and locate the best products and techniques for their specific applications.
This versatile label allows for the sensitive and specific detection of target DNA or RNA sequences by incorporating the digoxigenin-labeled deoxyuridine triphosphate into newly synthesized nucleic acid strands.
The Dig-11-dUTP can be detected using anti-digoxigenin antibodies, enabling visualization and quantification of the labeled targets.
Researchers can utilize PubCompare.ai's AI-driven platform to explore optimized protocols and methods for using Dig-11-dUTP, locating relevant literature, preprints, and patents, and identifying the best techniques and products through intelligent comparisons.
Nylon membranes are commonly used in conjunction with Dig-11-dUTP for techniques like Southern and Northern blotting, where the target nucleic acids are immobilized on the membrane and probed with the labeled nucleotide.
The Illustra GenomiPhi V2 DNA Amplification Kit can be used to amplify DNA samples, which may then be labeled with Dig-11-dUTP for in situ hybridization or other applications.
The In Situ Cell Death Detection Kit, which utilizes terminal deoxynucleotidyl transferase (TdT) to incorporate Dig-11-dUTP into DNA breaks, can be used to detect apoptotic cells.
The CHEF-DR®III Pulsed Field Electrophoresis System is another useful tool for separating large DNA fragments, which can then be analyzed using Dig-11-dUTP labeling.
Rhodamine-conjugated anti-digoxigenin antibodies can be used to visualize the Dig-11-dUTP-labeled targets, while 4-Thio-UTP is a related nucleotide analog that can also be used for labeling.
The QIAquick Gel Extraction Kit can be employed to purify DNA fragments labeled with Dig-11-dUTP, and the X-Omat processor is a common instrument used for developing autoradiographic films or chemiluminescent signals generated by Dig-11-dUTP detection.
Overall, Dig-11-dUTP is a versatile and widely used tool in molecular biology research, and PubCompare.ai can help researchers optimize their protocols and locate the best products and techniques for their specific applications.