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MitoTracker red CMXRos

MitoTracker red CMXRos is a fluorescent dye used to label and track mitochondria in living cells.
It selectively accumulates in active mitochondria, allowing for visualization and analysis of mitochondrial morphology and function.
This dye is widely used in cell biology research to study mitochondrial dynamics, apoptosis, and other mitochondria-related processes.
PubCompare.ai's AI-driven protocol comparison tool can help optimize your MitoTracker red CMXRos experiments by quickly identifying the most effective research protocols from literature, preprints, and patents.
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Most cited protocols related to «MitoTracker red CMXRos»

1
Visualizing Mitochondrial Dynamics in Embryonic Cells
Cited 20 times
MEFs were derived from e10.5 embryos. Embryos were mechanically dispersed by repeated passage through a P1000 pipette tip and plated with MEF media (DME, 10% FCS, 1× nonessential amino acids, 1 mM l-glutamine, penicillin/streptomycin [Life Technologies/GIBCO BRL]).
For visualization of mitochondria, the MEFs were either stained with 150 nM MitoTracker Red CMXRos (Molecular Probes) or infected with a retrovirus expressing EYFP fused to the presequence from subunit VIII of human cytochrome c oxidase, which directs EYFP to the mitochondrial matrix (a gift from R. Lansford, California Institute of Technology, Pasadena, CA) (Okada et al., 1999 (link)). To facilitate immortalization, the MEFs were later infected with a retrovirus expressing SV40 large T antigen (a gift from L. Jackson-Grusby, Massachusetts Institute of Technology) (Jat et al., 1986 (link)). Neither retroviral infection nor immortalization affected mitochondrial morphology. To label actin filaments, cells were fixed in 4% PFA and stained with 2.5 U/ml rhodamine-phalloidin (Molecular Probes). The stained cells were postfixed in 4% PFA.
For time-lapse confocal microscopy, cells were plated at low density onto chambered glass coverslips. Cells with culture medium were overlaid with light mineral oil and imaged in a 37°C chamber. EYFP-optimized filters and dichroics (q497lp, HQ500lp; Chroma) were used on a Zeiss 410 laser scanning confocal microscope (Carl Zeiss MicroImaging, Inc.)
TS cells from e3.5 blastocysts were derived using established protocols (Tanaka et al., 1998a (link)). Live cells were stained with MitoTracker Red (150 nM) and Syto16 (100 nM; Molecular Probes).
Chen H., Detmer S.A., Ewald A.J., Griffin E.E., Fraser S.E, & Chan D.C. (2003). Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. The Journal of Cell Biology, 160(2), 189-200.
Publication 2003
Amino Acids Blastocyst Cell Culture Techniques Cells COX8C protein, human Culture Media Embryo Glutamine Large T-Antigen Light Microfilaments Microscopy, Confocal Mitochondria Mitochondrial Inheritance MitoTracker red CMXRos Molecular Probes Oil, Mineral Penicillins Retroviridae Retroviridae Infections rhodamine-phalloidin Simian virus 40 Streptomycin
2
Multiparameter Imaging of Mitochondrial Function
Cited 14 times
Myocytes were loaded with dyes for >20 min on a microscope stage, incubated in Hepes-buffered solution (same composition as the storage solution) at 23°C, and imaged with a LSM-410 inverted confocal microscope using a Plan-Neofluar 63×/1.4N.A. oil immersion lens (Carl Zeiss, Inc.). Time scans were recorded from mitochondria arrayed along individual myofibrils in a multichannel line-scan mode with excitation at both 488 nm (for 2, 7-dichlorodihydrofluorescein diacetate [DCF], diaminofluorescein diacetate [DAF]-2, and calcein-AM) and 568 nm (for TMRE, TMRM [tetramethylrhodamine, methyl and ethyl ester, respectively], and MitoTracker® Red CMXRos; Molecular Probes, Inc.), collecting simultaneous fluorescence emission at 515–540 nm and >590 nm, respectively. Each image consisted of 512–1,024 line scans obtained at 2–230 Hz, each line comprising 512 pixels spaced at 0.050-μm intervals. The confocal pinhole was set to obtain spatial resolutions of 0.4 μm in the horizontal plane and 1.0 μm in the axial dimension. Some protocols were performed using 351 nm excitation, collecting 400–435 nm fluorescence emission, using a Zeiss c-apo 63×/1.3N.A. water immersion lens. Experiments were carried out at 23°C. Image processing was done using MetaMorph® software (Universal Imaging).
Zorov D.B., Filburn C.R., Klotz L.O., Zweier J.L, & Sollott S.J. (2000). Reactive Oxygen Species (Ros-Induced) Ros Release: A New Phenomenon Accompanying Induction of the Mitochondrial Permeability Transition in Cardiac Myocytes. The Journal of Experimental Medicine, 192(7), 1001-1014.
Publication 2000
Dyes Esters Fluorescence fluorexon HEPES Lens, Crystalline LINE-1 Elements Microscopy Microscopy, Confocal Mitochondria MitoTracker red CMXRos Molecular Probes Muscle Cells Myofibrils Radionuclide Imaging Submersion tetramethylrhodamine
3
Mitochondrial Morphology and Fusion Analysis
Cited 12 times
Mitochondrial morphology was scored in wild-type and mutant cells expressing a mitochondrial-targeted form of GFP (mito-GFP) either from the plasmid pVT100UGFP (provided by B. Westermann and W. Neupert, Universitaet Muenchen, Muenchen, Germany) or the plasmid pRS416 + preCox4-GFP (Otsuga et al. 1998). In some cases, mitochondria were labeled with a matrix-targeted form of the red fluorescent protein, mito-RFP (A.D. Mozdy and J.M. Shaw) or MitoTracker Red CMXRos (Molecular Probes, Inc.). Growth conditions were essentially as described (Bleazard et al. 1999). DAPI (4′,6-diamidino-2-phenylindole) staining was used to assess the presence/absence of mtDNA (Pringle et al. 1991). Mitochondrial fusion was examined essentially as described previously (Nunnari et al. 1997), except that mito-RFP was used in place of the vital dye MitoTracker Red CMXRos (Molecular Probes, Inc.). Digital microscopic images of cells were acquired using a Axioplan microscope or a Confocal microscope (Carl Zeiss, Inc.), as described previously (Hermann et al. 1998; Otsuga et al. 1998).
Mozdy A.D., McCaffery J.M, & Shaw J.M. (2000). Dnm1p Gtpase-Mediated Mitochondrial Fission Is a Multi-Step Process Requiring the Novel Integral Membrane Component Fis1p. The Journal of Cell Biology, 151(2), 367-380.
Publication 2000
Cells DAPI DNA, Mitochondrial Growth Disorders Microscopy Microscopy, Confocal Mitochondria Mitochondrial Fusion Mitomycin MitoTracker red CMXRos Molecular Probes Plasmids red dye CMXRos red fluorescent protein
4
Apoptosis and Mitochondrial Membrane Potential Analysis
Cited 7 times
Analysis of apoptosis by nuclear staining with Hoechst 33258 (Invitrogen) was performed as previously described (18 (link)). Annexin V/propidium iodide (PI) staining was performed using annexin Alexa 488 (Invitrogen) and PI as described (14 (link)). For analysis of cytochrome c release, mitochondrial and cytosolic fractions were isolated by the differential centrifugation method previously described (19 (link)), and probed by Western blotting for cytochrome c. For colony formation assays, the treated cells were plated in 12-well plates at appropriate dilutions, and allowed to grow for 10–14 days before staining with crystal violet (Sigma). For detection of mitochondrial membrane potential change, the treated cells were stained by MitoTracker Red CMXRos (Invitrogen) for 15 min at room temperature, and then analyzed by flow cytometry.
Dudgeon C., Wang P., Sun X., Peng R., Sun Q., Yu J, & Zhang L. (2010). PUMA induction by FoxO3a mediates the anticancer activities of the broad-range kinase inhibitor UCN-01. Molecular cancer therapeutics, 9(11), 2893-2902.
Publication 2010
Annexin A5 Annexins Apoptosis Biological Assay Cells Centrifugation Cytochromes c Cytosol Flow Cytometry Hoechst 33258 Membrane Potential, Mitochondrial Mitochondria MitoTracker red CMXRos Propidium Iodide Technique, Dilution Violet, Gentian
5
Mitochondrial Function in LPS-Tolerant Macrophages
Cited 6 times
LPS-tolerance might be associated with cell-energy adaptation. Therefore, several parameters of mitochondria were explored. As such, 200 nM of Mitotracker Red CMxROS (Molecular probe) was added to each well and incubated at 37°C for 15 min before removal. Then, cells were fixed with cold methanol at −20°C for 15 min, washed twice with PBS, and photographed by an IX81 inverted microscope (Olympus, Tokyo, Japan). Energy metabolism profiles with estimation of glycolysis were performed and assessments of mitochondrial oxidative phosphorylation with extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) were carried out by Seahorse XFp Analyzers (Agilent, Santa Clara, CA, USA) on macrophages at 1 × 105 cells/ well by Seahorse Wave 2.6 software as previously described (21 (link)).
Jaroonwitchawan T., Visitchanakun P., Dang P.C., Ritprajak P., Palaga T, & Leelahavanichkul A. (2020). Dysregulation of Lipid Metabolism in Macrophages Is Responsible for Severe Endotoxin Tolerance in FcgRIIB-Deficient Lupus Mice. Frontiers in Immunology, 11, 959.
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Publication 2020
Acclimatization Cells Cold Temperature Energy Metabolism Glycolysis Immune Tolerance Macrophage Methanol Microscopy Mitochondria Mitochondrial Inheritance MitoTracker red CMXRos Molecular Probes Oxidative Phosphorylation Oxygen Consumption Seahorses

Most recents protocols related to «MitoTracker red CMXRos»

1
Fluorescence Imaging of Yeast Mitochondria
Wild-type yeast (W303-1A) were maintained in Synthetic Defined (SD; 0.067% yeast nitrogen base, 2% glucose, drop-out mix with or without uracil). pDDGFP-LEU2D-ISCU and pDDGFP-LEU2D-PSS were transformed into S. cerevisiae using the S. C. EasyComp Transformation Kit (Invitrogen) according to manufacturer”s protocol and selected on SD-ura; SD without uracil. For fluorescence microscopy, cells were grown on SD-ura + 2% glucose media overnight at 30 °C. A 100 µl aliquot of cells were transferred to SD-ura supplemented with 2% galactose and lacking glucose for the induction of the genes and incubated at 30 °C for 4–6 h. To stain mitochondria and nuclei, Mitotracker Red CMXRos (final concentration of 200 nM) was added to the cells for 20 min, followed by DAPI (2 µg/ml) for 10 min, respectively. After incubation, cells were washed with PBS and loaded in a 1% agarose pad. Cells were imaged on a Zeiss Axio Imager.z2 microscope, using a Plan-Apochromat 100×/1.40 Oil Ph 3 M27 immersion oil objective lens. Excitation wavelength: mCherry (Mitotracker) 587 nm, EGFP 488 nm, and DAPI 353 nm, emission wavelength: mCherry 610 nm, EGFP 509 nm, and DAPI 465 nm. Exposure times for each channel were: mCherry 100 ms, EGFP 500 ms, DAPI 170 ms. Images were processed using linear adjustments (e.g., brightness/contrast) and deconvolved using Zen. Expression of PSS resulted in changes to the yeast cell morphology; additional pictures are provided in supplementary figure S6, Supplementary Material online.
Onuț-Brännström I., Stairs C.W., Campos K.I., Thorén M.H., Ettema T.J., Keeling P.J., Bass D, & Burki F. (2023). A Mitosome With Distinct Metabolism in the Uncultured Protist Parasite Paramikrocytos canceri (Rhizaria, Ascetosporea). Genome Biology and Evolution, 15(3), evad022.
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Publication 2023
Cell Nucleus Cells DAPI Galactose Glucose Induction, Genetic Lens, Crystalline Microscopy Microscopy, Fluorescence Mitochondria MitoTracker red CMXRos Nitrogen Saccharomyces cerevisiae Sepharose Stains Submersion sulofenur Uracil
2
Quantifying Mitochondrial Dynamics and Mitophagy

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Open the protocol to access the free full text link

Publication 2023
Cells Culture Media Flow Cytometry Fluorescence LysoTracker Microscopy, Confocal Mitochondria Mitomycin Mitophagy MitoTracker red CMXRos
3
Mitochondrial Localization of RIEP Protein
HeLa cells expressing vector alone or Flag-RIEP were prepared for IF following a previously described protocol and cultured on glass coverslips coated with 1% gelatin (15 (link)). To label mitochondria, prior to IF, cells were labeled with 200 μM MitoTracker Red CMXRos for 30 min. Generally, cells were fixed with 4% paraformaldehyde at 4 °C for 20 min, then permeabilized with 0.1% TritonX-100 in the cold for 20 min. Cells were washed 3 times using TBST, then incubated with Protein-Free (TBS) blocking buffer (Thermo Scientific, 37570) at RT for 1 h. Then cells were incubated with primary antibody: anti-Flag, NPM1, endogenous RIEP-1, RIEP-216, or RPA194 (1:500 dilution) overnight in the cold room. After washing three times with TBST, cells were incubated with the appropriate secondary fluorescence-conjugated antibody (Alexa Fluor 488 Goat anti-Rabbit IgG or Alexa Fluor 568 Goat anti-Mouse IgG, 1:500 dilution, Thermo Fisher) diluted with blocking buffer for 1 h at room temperature. Cells were mounted on slides with mounting buffer containing DAPI (Thermo Fisher) for imaging using a Zeiss Zen confocal microscope 700 with 63× oil objective. Images were recorded with the same settings. Image quantification was performed using Image J.
Feng S., Desotell A., Ross A., Jovanovic M, & Manley J.L. (2023). A nucleolar long “non-coding” RNA encodes a novel protein that functions in response to stress. Proceedings of the National Academy of Sciences of the United States of America, 120(9), e2221109120.
Publication 2023
alexa 568 alexa fluor 488 anti-IgG Buffers Cells Cloning Vectors Cold Temperature DAPI Fluorescent Antibody Technique Gelatins Goat HeLa Cells Immunoglobulins Microscopy, Confocal Mitochondria MitoTracker red CMXRos Mus NPM1 protein, human paraform Proteins Rabbits Technique, Dilution
4
Comprehensive Antibody Immunodetection Protocol
Antibodies recognizing the Flag tag (Sigma,F1804), SETX (Novus, NBP1-94712), RNA polymerase I (RPA194 (C-1) (Santa Cruz, sc-48385), fibrillarin (G-8) (Santa Cruz, sc-374022), nucleophosmin (NPM1, Forties Life Sciences A302-403A-T), C1QBP/P32 (Proteintech, 24474-1-AP), CHCHD2 (Proteintech, 66302-1-lg), GAPDH (Sigma-Aldrich, G9545), U2AF65 (Sigma-Aldrich, U4758), H3 (Abcam, ab8898), phospho-histone H2A.X(Ser139) (Cell signaling, 2577), and MitoTracker Red CMXRos (Invitrogen, M7512) were obtained from the indicated vendors. Human RIEP rabbit polyclonal antibodies were generated at Bethyl Laboratories/Fortis Life Sciences. RIEP-1 and RIEP-216 antibodies recognize peptides derived from the RIEP N terminus or C terminus, respectively. IGPAL-CA-630 (Sigma, I8896), cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail (Roche, 04693159001), and Pierce™ Protein A/G Magnetic Beads (Thermo Fisher scientific, 88802) were purchased from indicated vendors.
Feng S., Desotell A., Ross A., Jovanovic M, & Manley J.L. (2023). A nucleolar long “non-coding” RNA encodes a novel protein that functions in response to stress. Proceedings of the National Academy of Sciences of the United States of America, 120(9), e2221109120.
Publication 2023
Antibodies Edetic Acid fibrillarin G-substrate GAPDH protein, human H2AX protein, human Homo sapiens MitoTracker red CMXRos Novus NPM1 protein, human Peptides Protease Inhibitors Rabbits RNA Polymerase I SETX protein, human Staphylococcal Protein A
5
Immunofluorescence Analysis of Brain Hypoxia
Harvested brains were fixed in 4% paraformaldehyde and prepared into 10-mm frozen sections, and three mice were used in each group. The bEnd.3 cells were fixed on the slides with 4% paraformaldehyde. The samples were blocked with 10% goat serum at room temperature for 1 h, following which they were incubated overnight at 4°C with an appropriate primary antibody. The samples were then incubated with the corresponding secondary antibody at room temperature for 1 h and mounted with a medium containing DAPI (Zsbio, China). The following primary and secondary antibodies were used: Cox7c (1:100, 11411, Proteintech), CD31 (1:100, ab24590, Abcam), Alexa Fluor 488 (1:200, A32731, Invitrogen), Alexa Fluor 594 (1:200, A32744, Invitrogen). The detection of cell apoptosis and mitochondrial localization were performed according to the instructions of the One Step TUNEL Apoptosis Assay Kit (Beyotime, China) and Mito-Tracker Red CMXRos (Beyotime, China). A fluorescence microscope (200×; Eclipse 90i, Nikon, Japan) was used to acquire images. The immunofluorescence staining images of brains were from the cortex on the infarct side.
To assess hypoxia levels, three mice were used in each group in this experiment. Mice were injected with a 60 mg/kg hypoxyprobe (Burlington, United States) into the tail vein, following which mice were sacrificed and their brains were removed 1.5 h later. Tissue sections were incubated overnight at 4°C with anti-hypoxyprobe (1:100, FITC-conjugated mouse immunoglobulin G (IgG) monoclonal antibody). After washing, all sections were mounted on slides with a medium containing DAPI. The images were acquired using a fluorescence microscope (40×; Eclipse 90i, Nikon, Japan).
Jia J., Deng J., Jin H., Yang J., Nan D., Yu Z., Yu W., Shen Z., Lu Y., Liu R., Wang Z., Qu X., Qiu D., Yang Z, & Huang Y. (2023). Effect of Dl-3-n-butylphthalide on mitochondrial Cox7c in models of cerebral ischemia/reperfusion injury. Frontiers in Pharmacology, 14, 1084564.
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Publication 2023
Alexa594 alexa fluor 488 Antibodies Apoptosis Biological Assay Brain Cortex, Cerebral DAPI Decompression Sickness Fluorescein-5-isothiocyanate Fluorescent Antibody Technique Frozen Sections Goat Hypoxia Immunoglobulin G Immunoglobulins Infarction In Situ Nick-End Labeling Microscopy, Fluorescence Mitochondria MitoTracker red CMXRos Monoclonal Antibodies Mus paraform Serum Tail Tissues Veins

Top products related to «MitoTracker red CMXRos»

1
Mitotracker red cmxros by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, Japan, China, Italy, Canada, Spain, France, Belgium
MitraTracker Red CMXRos is a fluorescent dye that can be used to stain mitochondria in live cells. It is a cell-permeant dye that accumulates in active mitochondria, enabling the visualization and analysis of mitochondrial structure and function.
2
Mito tracker red cmxros by Beyotime
Sourced in China, United States, Japan
Mito-Tracker Red CMXRos is a fluorescent dye that specifically stains mitochondria in live cells. It is a cationic lipophilic dye that accumulates in the mitochondrial matrix in proportion to the membrane potential.
3
Mitotracker green fm by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, Japan, Australia, France, Italy
MitoTracker Green FM is a fluorescent dye that specifically labels mitochondria in live cells. It passively diffuses across the plasma membrane and accumulates in active mitochondria. The dye exhibits bright green fluorescence upon binding to mitochondrial lipids.
4
Hoechst 33342 by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, Japan, China, France, Canada, Spain, Belgium, Italy, Australia, Austria, Denmark, Netherlands, Switzerland, Ireland, New Zealand, Portugal, Brazil, Argentina, Singapore, Poland, Ukraine, Macao, Thailand, Finland, Lithuania, Sweden
Hoechst 33342 is a fluorescent dye that binds to DNA. It is commonly used in various applications, such as cell staining and flow cytometry, to identify and analyze cell populations.
5
Mitotracker red cmxros by Cell Signaling Technology
Sourced in United States
MitoTracker Red CMXRos is a fluorescent dye that selectively accumulates in the mitochondria of live cells. It is a cationic dye that is readily sequestered by active mitochondria with intact membrane potentials.
6
Mitosox red by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Canada, Australia, Japan, Switzerland, Italy, France, Spain
MitoSOX Red is a fluorogenic dye designed to measure superoxide in the mitochondria of live cells. It is readily oxidized by superoxide but not by other reactive oxygen species. The oxidized product is highly fluorescent, allowing for the detection and quantification of mitochondrial superoxide.
7
Mitotracker green by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, Australia, Japan, Canada, Italy
MitraTracker Green is a fluorescent dye used to label and monitor mitochondria in live cells. It passively diffuses across the cell membrane and accumulates in active mitochondria. The dye exhibits enhanced fluorescence upon binding to the mitochondrial membrane potential.
8
4 6 diamidino 2 phenylindole (dapi) by Merck Group
Sourced in United States, Germany, Japan, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, Canada, Switzerland, Spain, Australia, Denmark, India, Poland, Israel, Belgium, Sweden, Ireland, Netherlands, Panama, Brazil, Portugal, Czechia, Puerto Rico, Austria, Hong Kong, Singapore
DAPI is a fluorescent dye that binds strongly to adenine-thymine (A-T) rich regions in DNA. It is commonly used as a nuclear counterstain in fluorescence microscopy to visualize and locate cell nuclei.
9
4 6 diamidino 2 phenylindole (dapi) by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, Japan, China, Canada, Italy, Australia, France, Switzerland, Spain, Belgium, Denmark, Panama, Poland, Singapore, Austria, Morocco, Netherlands, Sweden, Argentina, India, Finland, Pakistan, Cameroon, New Zealand
DAPI is a fluorescent dye used in microscopy and flow cytometry to stain cell nuclei. It binds strongly to the minor groove of double-stranded DNA, emitting blue fluorescence when excited by ultraviolet light.
10
Lysotracker red dnd 99 by Thermo Fisher Scientific
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LysoTracker Red DND-99 is a fluorescent dye that selectively stains acidic organelles, such as lysosomes, in live cells. It can be used to visualize and monitor the distribution and activity of lysosomes within the cellular environment.

More about "MitoTracker red CMXRos"

MitoTracker red CMXRos is a vital fluorescent dye used extensively in cell biology research to study mitochondrial dynamics, function, and related processes.
This lipophilic cation selectively accumulates in active mitochondria, enabling visualization and analysis of mitochondrial morphology, distribution, and activity.
The dye is often used in conjunction with other fluorescent probes like MitoTracker Green FM, Hoechst 33342, MitoSOX Red, and LysoTracker Red DND-99 to provide a comprehensive assessment of cellular organelles and processes.
Researchers leverage the specificity of MitoTracker red CMXRos to track mitochondrial movement, monitor apoptosis, and investigate mitochondrial respiratory function.
PubCompare.ai's AI-driven protocol comparison tool can help optimize your MitoTracker red CMXRos experiments by quickly identifying the most effective research protocols from literature, preprints, and patents.
This intelligent assistant takes the guesswork out of your experiments, allowing you to make informed decisions and obtain reliable results.
Whether you're studying mitochondrial dynamics, mitochondria-related signaling pathways, or other cellular processes, PubCompare.ai can help you locate the best protocols and optimize your experimental design.