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Brine

Brine is a saline solution, typically composed of water and dissolved salts, often used in various industrial and scientific applications.
This high-concentration salt solution can be found in natural environments, such as salt lakes and oceans, or artificially produced for specific purposes.
Brine plays a crucial role in diverse fields, including food processing, water treatment, oil and gas extraction, and medical research.
The optimization of brine protocols, using AI-driven analysis and comparison, can enhance reproducibility and accuracy in brine-related research and applications, leading to more efficient and effective brine utilization.
PubCompare.ai offers a powerful tool to locate the best brine protocols from literature, pre-prints, and patents, empowering researchers and professionals to experience the benefits of AI-assisted brine optimization.

Most cited protocols related to «Brine»

Chemical synthesis of potential inhibitors. Reagents and solvents were from Aldrich, Alfa Aesar or Acros. Reactions were monitored by TLC, which was performed on precoated aluminum-backed plates (Merck, silica 60 F254). Melting points were determined using a Leica Galen III hot-stage melting point apparatus and microscope. Infrared spectra were recorded from Nujol mulls between sodium chloride discs, on a Bruker Tensor 27 FT-IR spectrometer. NMR spectra were acquired using a Bruker DPX500 NMR spectrometer. Chemical shifts (δ) are given in ppm, and the multiplicities are given as singlet (s), doublet (d), triplet (t), quartet (q), multiplet (m), broad (br). Coupling constants J are given in Hz (± 0.5 Hz). High resolution mass spectra (HRMS) were recorded using a Bruker MicroTOF spectrometer. The purity of all compounds synthesized were ≥95% as determined by analytical reverse-phase HPLC (Ultimate 3000). Daminozide (Alar) and compound 28 are commercially available. The synthesis and characterisation of compounds 2225 , 2526 (link), 2727 , 3628 , 3729 and 3826 (link) has been reported. The synthesis of compounds 31-35, 39-41 and 13C NMR spectra for 22, 23, 24, 26, 31-35 are given in the Supporting Information.
4-(2,2,2-Trimethylhydrazinyl)-4-oxobutanoate 22. The synthesis of compound 22 was as reported25 , thus reaction of daminozide (500mg, 3.1 mmol) with methyl iodide (700mg, 0.31 mL, 5.0 mmol) gave 22 as a white solid (75% yield), mp: 137-138 °C (lit.1 137-138.5 °C); 1H NMR (500 MHz, MeOD): δ 2.40 (t, J = 6.5 Hz, 2H), 2.51 (t, J = 6.5 Hz, 2H), 3.56 (s, 9H); 13C NMR (125 MHz, MeOD): δ 28.5, 29.1, 56.1, 170.4, 173.4; IR (neat) υ/cm−1: 3405, 3312, 1729, 1693; HRMS (m/z): [M]+ calcd. for C7H15N2O3, 175.1077; found, 175.1081.
General procedure for the coupling of hydrazine to succinic anhydride. To a stirred solution of the appropriate hydrazine (1 equiv.) in acetonitrile (5 mL) was added dropwise a solution of succinic anhydride (200 mg, 2.0 mmol, 1 equiv.) in acetonitrile (5 mL). The mixture was stirred at room temperature for 24 h, after which the solvent was evaporated in vacuo and the resulting crude purified using semipreparative reverse-phase HPLC, performed on a phenomenex C18 column (150 mm × 4.6 mm). Separation was achieved using a linear gradient of solvent A (water + 0.1% CF3CO2H) and solvent B (acetonitrile + 0.1% CF3CO2H), eluting at a flow rate of 1 mL/min and monitoring at 220 nm: 0% B to 40% B over 30 min.
4-(2-Methylhydrazinyl)-4-oxobutanoic acid 23. Compound 23 is a colourless oil (63% yield), 1H NMR (500 MHz, DMSO-d6): δ 2.35 (t, J = 7.0 Hz, 2H), 2.68 (t, J = 7.0 Hz, 2H), 2.98 (s, 3H), 4.76 (s, 1H), 7.74 (s, 1H); 13C NMR (125 MHz, DMSO-d6): δ 28.3, 29.1, 170.1, 173.6; IR (neat) υ/cm−1: 33 3219, 3057, 1708, 1632; HRMS (m/z): [M+Na]+ calcd. for C5H10N2NaO3, 169.0584; found, 169.0577.
4-Hydrazinyl-4-oxobutanoic acid 24. Compound 24 is a colourless oil (87% yield), 1H NMR (500 MHz, DMSO-d6): δ 2.34 (t, J = 7.0 Hz, 2H), 2.60 (t, J = 7.0 Hz, 2H), 5.86 (s, 1H), 8.99 (s, 1H); 13C NMR (125 MHz, DMSO-d6): δ 28.2, 29.1, 170.8, 173.9; IR (neat) υ/cm−1: 3303, 3290, 3199, 1712, 1624; HRMS (m/z): [M-H]- calcd. for C4H7N2O3, 131.0462; found, 131.0468.
4-Oxo-4-(1,2,2-trimethylhydrazinyl)butanoic acid 26. Compound 26 is a white solid (56% yield), mp: 97-98 °C, 1H NMR (500 MHz, DMSO-d6): δ 2.37 (t, J = 7.0 Hz, 2H), 2.66 (t, J = 7.0 Hz, 2H), 2.74 (s, 3H), 11.98 (s, 1H); 13C NMR (125 MHz, DMSO-d6): δ 28.2, 29.8, 43.4, 48.7, 173.5, 175.0; IR (neat) υ/cm−1: 2958, 1723, 1615; HRMS (m/z): [M+Na]+ calcd. for C7H14N2NaO3, 197.0897; found, 197.0895.
4-((Dimethylamino)oxy)-4-oxobutanoic acid 29. N,N-Dimethylhydroxylamine (39 mg, 0.63 mmol, 1.1 equiv. ) was added to a solution of 4-(tert-butoxy)-4-oxobutanoic acid (100 mg, 0.57 mmol, 1 equiv.), hydroxybenzotriazole (100 mg, 0.74 mmol, 1.3 equiv.), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide ( 140 mg, 0.74 mmol, 1.3 equiv.) and diisopropylethylamine ( 0.2 mL, 1.14 mmol, 2.0 equiv.) in CH2Cl2 (10 mL). The reaction was stirred at room temperature overnight, washed with water, HCl 1N, brine, dried on MgSO4. The organic phase was evaporated in vacuo and purified by chromatography (MeOH/CH2Cl2 0.5/9.5) to obtain 110 mg of tert-butyl 4((dimethylamino)oxy)-4-oxobutanoate (90% yield). CF3CO2H (0.04 ml, 0.37 mmol, 4 equiv.) was added to a solution of tert-butyl 4((dimethylamino)oxy)-4-oxobutanoate (20 mg, 0.09 mmol, 1 equiv.) in CH2Cl2 (1.5 ml). The reaction was stirred at room temperature for 4h and evaporated in vacuo to give 14 mg of 29 (yield 95%). 1H NMR (500 MHz, CD3OD) δ 2.59 (s, 6H), 2.57 (s, 4H); 13C NMR (500 MHz, CD3OD) δ 176.2, 172.0, 48.5, 29.9; IR (neat) 3341, 2485,1717, 1120, 1026, 975 cm−1; HRMS (m/z):[M+]calcd. for C6H11NO4 161.0688; found 161.0923.
N‘1, N‘1, N‘4, N‘4-Tetramethylsuccinohydrazide 30. A solution of succinic acid (100 mg, 0.85 mmol, 1 equiv.), hydroxybenzotriazole (350 mg, 2.11 mmol, 2.3 equiv.), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (421 mg, 2.11 mmol, 2.3 equiv.), diisopropylethylamine (0.6 mL, 3.4 mmol, 4 equiv.) and 1,1-dimethylhydrazine (0.16 mL, 2.04 mmol, 2.2 equiv.) in CH2Cl2 (20 mL) was stirred at room temperature overnight. CH2Cl2 (10 mL) was added and the reaction mixture was washed with water, a saturated solution of NaHCO3, brine and dried on MgSO4. The organic phase was evaporated in vacuo and purified by chromatography (MeOH/ CH2Cl2 1/9) to give 77 mg of 30 (45% yield). 1H NMR (500 MHz, CD3OD) δ 2.87 (s, 6H), 2.65 (s, 2H); 13C NMR (500 MHz, CD3OD) δ 178.2, 43.8, 27.6; IR (neat) 3356, 2485, 2071, 1695, 1120, 1027, 974 cm−1; HRMS (m/z):[(M-2CH3)]calcd. for C6H14N4O2, 174.1117; found, 174.1022.
Publication 2012
Three extremophilic microbes, previously isolated from the deep-sea anoxic brine lakes, were selected as part of a genome-sequencing project due to their phylogenetic position, peculiar features and unique biotope. Analysis of their draft genomes provides us with a first glimpse on some of their unusual characteristics and the ways they cope with living in such a harsh environment [11 (link)-13 (link)].
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Publication 2013
Anoxia brine Extremophiles Genome
Sediment cores (the top 5 cm), composed mainly of salt crystals and brine, were sampled in June 2016 from the final crystallizer pond Vasca #27 of the solar saltern system Saline della Laguna (Sicily, Italy). Chemical analysis of environmental samples, cultivation conditions, microbial community analysis, genome sequencing and assembly, phylogenomic studies, proteome analysis, analysis of polymer hydrolysis metabolites, quantitative PCR, field emission scanning and transmission electron microscopies and fluorescence microscopy are detailed in SI Appendix, Materials and Methods.
Publication 2020
brine Genome Hydrolysis Microbial Community Microscopy, Fluorescence Polymers Proteome Saline Solution Sodium Chloride Transmission Electron Microscopy
Dried cysts were performed as indicated above, and incubated (1 g cyst per liter) in a hatcher at 28–30°C with strong aeration, under a continuous light regime. Approximately 12 h after hatching the phototropic nauplii were collected with a pipette from the lighted side and concentrated in a small vial. Ten brine shrimp were transferred to each well using adequate pipettes. Each test consisted of exposing groups of 10 Artemia aged 12 h to various concentrations of the toxic compound. The toxicity was determined after 12 h (mainly nauplii in instar I/II), 24 h (nauplii in instar II/III) and 48 h (mainly nauplii in instar III/IV) of exposure.
The numbers of survivors were counted and percentage of deaths were calculated. Larvae were considered dead if they did not exhibit any internal or external movement during several seconds of observation.
The larvae did not receive food. To ensure that the mortality observed in the bioassay could be attributed to bioactive compounds and not to starvation; we compared the dead larvae in each treatment to the dead larvae in the control. In any case, hatched brine shrimp nauplii can survive for up to 48 h without food [15 ] because they still feed on their yolk-sac [10 (link)]. However, in cases where control deaths were detected, the percentage of mortality (% M) was calculated as: % M = percentage of survival in the control - percentage of survival in the treatment.
Publication 2002
Artemia Biological Assay Cyst Food Larva Movement Neoplasm Metastasis Survivors Yolk Sac
Zebrafish used in this study was Tübingen strain that was initially obtained from the Zebrafish International Resource Center and propagated in our lab according to the following procedure. Fish were kept at constant water temperature (28°C), photoperiod (14L:10D, lights on 9:00, lights off at 23:00), pH (7.2), and salinity (conductivity 500–1200 μS) in automatic controlled zebrafish rearing systems (Aquatic Habitats Z-Hab Duo systems, FL, USA). Fish were fed three times daily to satiation with a commercial food (Otohime B2, Reed Mariculture, CA, USA) contained high protein content and supplemented with newly hatched artemia (Brine Shrimp Direct, UT, USA). Fertilized eggs were collected from natural spawning of paired healthy fish in the morning once lights were turned on. Experimental protocols were approved by The Institutional Animal Care and Use Committee at East Carolina University.
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Publication 2015
Artemia Electric Conductivity Fishes Food Institutional Animal Care and Use Committees Light Proteins Salinity Satiation Strains Zebrafish Zygote

Most recents protocols related to «Brine»

In this study, the brine employed for the experiments was prepared by dissolving various salts (with a purity exceeding 99 %) in distilled water as outlined in Table 1. The research used a reference fluid with a salinity of 40,000 ppm as a base case, and also examined the impact of low salinity (4000 ppm) and high salinity (80,000 ppm) water to explore salinity-related effects [17 ].

The composition of different brines (in ppm) that are used in this study.

Table 1
Ion4000 ppm40,000 ppm80,000 ppm
Na+17241724334486
Cl19811980739614
K+40400800
HCO3-550100
Mg2+21421434286
Ca2+46460920
SO42-15014972994
TDS41624165983318
Ionic strength, mol/L0.0830.8321.664
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Publication 2024
The RO brine of petrochemical integrated wastewater was collected from a petrochemical factory that mainly produces terephthalic acid (Dalian, China). The primary characteristics of the brine are listed in Table 1. Brie y, this brine is transparent that free from visible insoluble impurities and strong odor. It exhibits a COD value ranging from 35 to 45 mg/L, while the total dissolved solids (TDS) range between 5500 and 6000 mg/L. The conductivity of the brine falls within the range of 7500 to 8400 µs/cm, making it suitable for electrochemical treatment. Based on the characteristics of the brine, the main reaction equations in the electrophoresis tank can be determined as follows: the rst combination is similar to that in the chlor-alkali industry (Moussallem et al. 2008) , where the electrolysis of chlorine salt solutions produces OH -, hydrogen and chlorine (Equations 1-2); the second combination involves the cleavage of salt and water, resulting in the production of H + , OH -, H 2 , and O 2 (Equations 3-4) (Kumar et al. 2019) .
Publication 2024
Brine process water (as the standard brine solution) contaminated with MPs at a level of 3671 ± 174 particles/L was obtained from kimchi manufacturing plants in Gwangju, Korea, to investigate the MP removal effect. MPs in the provided standard brine sample were quantified following the description in Section 2.3. Brine process water (i.e., the standard brine solution used in all processes) was used after continuous stirring to obtain a MP-homogenized standard brine solution. Three types of materials for filtration were examined in this study: i) a glass microfiber filter (Whatman Grade GF 10 glass microfiber filter); ii) an STS filter (20-μm stainless-steel filter mesh, Sintered 316L, Hengko Technology Co., Ltd., China); and iii) an SI filter (20-μm BioTect Ultra ceramic, Doulton Ltd., United Kingdom). For the standard brine samples filtered using each filter, MP precision analysis was performed by recovering the number of filtered MP particles.
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Publication 2024
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A container filled with saline solution was used to hatch the eggs of brine shrimp under suitable conditions of temperature, light, and aeration. The eggs hatched into nauplii (larval stage of brine shrimp) within a day. A series of test solutions with different concentrations of the sample were prepared by diluting them in the solvent. 10 brine shrimp nauplii were added to each container, including the control using a micropipette. The nauplii are allowed to swim freely in the test solutions. The containers were incubated under suitable conditions for 24 hours. The temperature and lighting conditions were maintained. After the incubation period, the containers were examined to determine the number of surviving brine shrimp nauplii in each concentration and control. The lethality or mortality rate is calculated by counting the number of dead nauplii (5, 18, 19) .
Publication 2024
The brine shrimp lethality bioassay was used to determine the cytotoxicity of the extracts. Artemia salina shrimps were produced using Artemia salina eggs in a container filled with brine solution, which is made by dissolving 38 g of sea salt in 1000 mL of distilled water and adjusting the pH to 8.5 using 1ml NaOH. The process took 48 hours with continuous aeration. Live-shrimps were gathered and utilized in the test (Rafshanjani et al., 2014) . In each test tube, 4.5 mL of the brine solution were added. The extract of E. indica was diluted appropriately in accordance with concentrations. The shrimp test tubes received 0.5 mL of the diluted solution of E. indica. Each test tube had ten live shrimp introduced to it using a glass dropper tube. After 24 hours, the surviving (larvae) shrimps were counted, and the lethality concentration LC50 determined. % death = (number of dead nauplii /(number of dead nauplii + number of live nauplii)) × 100
Publication 2024

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NaCl is a chemical compound commonly known as sodium chloride. It is a white, crystalline solid that is widely used in various industries, including pharmaceutical and laboratory settings. NaCl's core function is to serve as a basic, inorganic salt that can be used for a variety of applications in the lab environment.
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More about "Brine"

Brine is a high-concentration saline solution, typically composed of water and dissolved salts, such as sodium chloride (NaCl).
This type of solution is commonly found in natural environments like salt lakes and oceans, but it can also be artificially produced for various industrial and scientific applications.
Brine plays a crucial role in diverse fields, including food processing, water treatment, oil and gas extraction, and medical research.
The optimization of brine protocols, using AI-driven analysis and comparison, can enhance reproducibility and accuracy in brine-related research and applications, leading to more efficient and effective brine utilization.
This process can involve the use of analytical techniques like Prep-HPLC, which utilizes a reversed-phase C18 stationary phase (e.g., Boston Prime C18, XB-C18) to separate and analyze the components of brine solutions.
Additionally, the use of solvents like DMSO can be employed to enhance the solubility and extraction of specific analytes from brine samples.
PubCompare.ai offers a powerful tool to locate the best brine protocols from literature, pre-prints, and patents, empowering researchers and professionals to experience the benefits of AI-assisted brine optimization.
This can include the use of column chemistries like Synergi C18, XBridge C18, Gemini C18, and Luna C18 to optimize the separation and analysis of brine components.
By harnessing the power of AI-driven protocol comparisons, researchers and professionals can enhance the reproducibility and accuracy of their brine-related work, leading to more efficient and effective brine utilization across a wide range of applications.
Experince the power of AI-assisted brine optimization today and discover how PubCompare.ai can help you navigate the world of brine research and development.