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Clorox

Clorox is a household cleaning and disinfecting brand known for its bleach-based products.
The Clorox Company manufactures a wide range of cleaning solutions, including liquid bleach, disinfecting wipes, and cleaning sprays.
These products are commonly used for sanitizing surfaces, removing stains, and killing germs.
Clorox has been a trusted name in household cleanning for over a century, offering effective and convenient solutions for a variety of cleaning needs.
Experinece the power of Clorox's trusted brand today.

Most cited protocols related to «Clorox»

1
Establishing Homozygous Transgenic Petunia Lines
Transformants were transferred into soil (Metro-Mix 200, Sun Gro, Bellevue, WA, USA) and grown under artificial lighting (∼40 μM m−2 s−1, 16 h photoperiod, 25°C). The stigma of each flower was artificially pollinated and the flower without petals was enveloped with tape to avoid cross-pollination. T1 seeds were collected and sown on MS (Sigma, USA) medium with 20 mg ml−1 hygromycin (Sigma, USA) for the further selection of homozygous lines. The following procedure was performed: T1 seeds were first surface-sterilised in 15% bleach (Clorox, USA) with 0.01% Tween-20 (Sigma, USA) for 20 min and then 70% ethanol for 45sec, followed by three washes with sterile distilled water. The plates (20 seeds/plate) were kept at room temperature under continuous low fluorescence light (∼40 μM m−2 s−1) for 21 days. T1 seeds which displayed a 3∶1 ratio of survival on hygromycin medium were retained and moved to pots for harvesting T2 seeds. One hundred seeds from each line were germinated again on MS medium containing 20mg ml−1 hygromycin, and lines having 100% survival were identified as homozygous and used for further experiments.
PCR amplification was performed for further identifying transgenic lines. Genomic DNA of the petunia transformed Arabidopsis etr1-1 gene was extracted using a hexadecyltrimethylammonium bromide (CTAB) method, as described previously [19] (link). PCRs were carried out in a total reaction volume of 20 µl, according to the procedure as the following: 5 min at 95°C followed by 40 cycles of 30 s at 94°C, 30 s at 56°C, and 1min at 72°C. Primer pairs: 5′-CCATCACACTAAATCTTGCACCA-3′ and reverse 5′- TTCGGTATGCCCGACTGTTTAG- 3′ were used. 1.0% agarose gel electrophoresis was performed using standard protocols.
Wang H., Stier G., Lin J., Liu G., Zhang Z., Chang Y., Reid M.S, & Jiang C.Z. (2013). Transcriptome Changes Associated with Delayed Flower Senescence on Transgenic Petunia by Inducing Expression of etr1-1, a Mutant Ethylene Receptor. PLoS ONE, 8(7), e65800.
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Publication 2013
Animals, Transgenic Arabidopsis Cetrimonium Bromide Clorox Electrophoresis, Agar Gel Ethanol Fluorescence Genes Genome Homozygote hygromycin A Light Marijuana Abuse Oligonucleotide Primers Petunia Plant Embryos Pollination Polymerase Chain Reaction Sterility, Reproductive Tween 20
2
Micropropagation of Pineapple Cultivars
Crown meristems and slips of three pineapple varieties (Tainong 11, MD2 and Tainong 21) were used as explants for the initiation stage of micro-propagation. The explants were washed thoroughly with commercial antibacterial liquid detergent for 30 min followed by washing with running tap water for 4 h. The explants were sterilized with 75% ethanol, followed by three washes with sterilized distilled water under aseptic conditions. The explants were divided into six sets, five of which were surface sterilized with different concentrations of Clorox (NaOCl, 10% and 15%) for different amounts of time (5, 10 and 15 min). The remaining set was surface sterilized with 0.1% mercuric chloride (HgCl2) for 10 min. All six sets were washed with sterilized distilled water three times. Before slicing the samples for inoculation into medium, the explants were dried on sterilized filter paper under aseptic conditions.
Bud initiation was tested with different concentrations of BAP (4 and 5 mg/l) combined with 0.2 mg/l NAA and 0.2 mg/l IAA in full strength MS [21 (link)] medium (pH = 5.8). Plantlets obtained from the subculturing process were treated with different rooting hormones (1 mg/l NAA and 1 mg/l IAA) in liquid and solid full strength MS medium for root induction. Full strength MS medium without added hormone was also tested for rooting.
Cultures were maintained under a light intensity of 3000 Lux and a day/night cycle of 8/16 h at 25 ± 2 °C in a controlled environment. All growth media used in the above experiments were sterilized by autoclaving at 121 °C for 20 min.
Priyadarshani S.V., Hu B., Li W., Ali H., Jia H., Zhao L., Ojolo S.P., Azam S.M., Xiong J., Yan M., ur Rahman Z., Wu Q, & Qin Y. (2018). Simple protoplast isolation system for gene expression and protein interaction studies in pineapple (Ananas comosus L.). Plant Methods, 14, 95.
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Publication 2018
1-naphthylarsonic acid Anti-Bacterial Agents Asepsis Clorox Culture Media Detergents Environment, Controlled Ethanol Hormones Light Mercuric Chloride Meristem Pineapple Transcription Initiation, Genetic Vaccination
3
Arabidopsis thaliana Pathogen Assay Protocol
Arabidopsis thaliana ecotype Colombia (Col-0) was used as a wild-type plant in this study. The male sterile coi1-17 line [41 (link)] was obtained from Dr. Barbara Kunkel (Washington University, St. Louis MO) and maintained as a heterozygous stock. The homozygous coi1-17 line was selected by growing the seeds from segregating lines on one-half Murashige and Skoog medium (MS) containing 10 μM methyl jasmonate (MeJA; Bedoukian Research Inc., Danbury, CT, U.S.A.) for seven days, and then transferring to one-half MS medium without MeJA. The sid2-2 (eds16) line [42 (link),43 (link)] was obtained from Dr. Frederick Ausubel (Massachusetts General Hospital, Boston, MA). The fls2 line [36 (link)] was obtained from Dr. Yuki Ichinose (Okayama University, Okayama, Japan).
Arabidopsis seeds were sterilized using bleach. In brief, 100-200 seeds were incubated with 70% ethanol for 5 min in a microcentrifuge tube and then incubated with 20% (v/v) commercial bleach containing 6% sodium hypochlorite (Clorox Co., Oakland, CA) containing 0.1% Tween 20 (Sigma-Aldrich, St. Louis, MO, U.S.A.). After surface sterilization, seeds were washed with sterile distilled H2O at least four times and germinated on one-half strength MS medium containing Gamborg vitamins (PhytoTechnologies Laboratories, Shawnee Mission, KS, U.S.A.) solidified with 0.3% Phytagel (Sigma-Aldrich) in deep Petri plates (100 mm × 25 mm). The MS plates were dried overnight in the hood with closed lid before transferring the surface-sterilized seeds. The MS plates with seeds were kept for two days at 4°C to break the dormancy and were further incubated at 24°C with a light intensity of 150-200 μE m-2 sec-1 and a 12 h light/12 h dark photoperiod, and the seedlings, two weeks post-germination, were used for pathogen assays.
Ishiga Y., Ishiga T., Uppalapati S.R, & Mysore K.S. (2011). Arabidopsis seedling flood-inoculation technique: a rapid and reliable assay for studying plant-bacterial interactions. Plant Methods, 7, 32.
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Publication 2011
Arabidopsis Arabidopsis thalianas Biological Assay Clorox Ecotype Ethanol Germination Heterozygote Homozygote Light M-200 Males methyl jasmonate Pathogenicity Plant Embryos Plants Seedlings Sodium Hypochlorite Sterility, Reproductive Tween 20 Vitamins
4
Isolation of Bacterial Endophytes from Appalachian Plants
Bacterial endophytes were isolated from plants grown in the foothills of the Appalachian Mountains in Central Virginia, USA (geographic location: 37.125372, −79.298415). The soil was not fertilized. Healthy plants were selected randomly from the natural environment. Plants were taken from the field and brought to the lab for isolation or kept in a refrigerator temporarily. The plants were first washed with tap water to remove the soil, and then surface was sterilized with 5% Clorox® bleach (The Clorox Company, Oakland, CA, USA) containing 8.5% NaOCl with a drop of Tween 20 for 5 min. Finally, the plants were rinsed with sterile water five times.
To confirm the sterilization efficacy, 50 µL of the last rinsate were plated on Luria agar (LA) medium. The surface-sterilized plant was divided into the root, leaf and stem, which were ground individually with sterile water. The ground tissues were centrifuged at 2000 rpm for 3 min to remove the debris. The supernatant was diluted to 10 ×, 100 × and 1000 × with sterile water, and then 50 µL were spread on LA plates. The plates were placed at 28 °C for 2–5 days. Different individual colonies were streaked onto a fresh LA plate for purification and for producing a glycerol stock for long-term storage.
Mei C., Chretien R.L., Amaradasa B.S., He Y., Turner A, & Lowman S. (2021). Characterization of Phosphate Solubilizing Bacterial Endophytes and Plant Growth Promotion In Vitro and in Greenhouse. Microorganisms, 9(9), 1935.
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Publication 2021
Agar Bacteria Clorox Endophytes Glycerin isolation Plant Leaves Plant Roots Plants Stem, Plant Sterility, Reproductive Tissues Tween 20
5
Arabidopsis Phosphate Homeostasis Assay
Arabidopsis thaliana accession Col-0 was used. All seeds were surface-sterilized with 70% bleach (Clorox, Oakland, CA), 0.2% Tween-20 (MilliporeSigma, St. Louis, MO) for 8 minutes, and rinsed 3 times with sterile distilled water to eliminate any seed-borne microbes on the seed surface. Seeds were stratified at 4°C in the dark for 2 days. Plants were germinated on vertical square 12 X 12 cm agar plates (Fisher Scientific, Hampton, NH) with Johnson medium (JM; [4 (link)]) containing 0.5% sucrose (MilliporeSigma, St. Louis, MO) and 1,000 μM Pi, for 7 days. Then, 10 plants were transferred to each vertical agar plate with amended JM lacking sucrose at one of the following experimental Pi concentrations: 0, 10, 30, 50, 100, or 1,000 μM Pi. The SynCom was spread on the agar prior to transferring plants. Each experiment included unplanted agar plates with SynCom for each media type (designated NP) and uninoculated plates with plants for each media type (designated NB). Plants were placed in randomized order in growth chambers and grown under a 16-hour dark/8-hour light regime at 21°C day/18°C night for 12 days (the period of time it takes roots to reach the bottom of the plate).
Finkel O.M., Salas-González I., Castrillo G., Spaepen S., Law T.F., Teixeira P.J., Jones C.D, & Dangl J.L. (2019). The effects of soil phosphorus content on plant microbiota are driven by the plant phosphate starvation response. PLoS Biology, 17(11), e3000534.
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Publication 2019
Agar Arabidopsis thalianas Clorox Light Plant Embryos Plant Roots Plants Sterility, Reproductive Sucrose Tween 20

Most recents protocols related to «Clorox»

1
Microwave Inactivation of Penicillium roqueforti Spores
Not available on PMC !

Example 3

Penicillium roqueforti spores were suspended in water. Four DEE chemical compositions were evaluated: (1) 0.06 M copper (II) ions in water, (2) 1 wt.-% surfactant and 10 wt.-% PCSR, (3) 1 wt.-% surfactant and 1 wt.-% PCSR, and (4) 0.5 wt.-% bleach. OxiClean was used as the PCSR and Tween 80 as the surfactant. Clorox was used as bleach. Each DEE composition was added to 0.1 mg/ml suspension of mold spores and exposed to 2.45 GHz microwave for 10 s. After exposure, the cells were centrifuged, washed to remove the DEE chemicals and then plated on Petrifilm and cultured. With the DEE composition 0.06 M copper (II) ions in water and 1 wt.-% surfactant and 10 wt.-% sodium percarbonate, a 6-7 log reduction in P. roqueforti spores (6-7 log kill levels) was realized.

US11872322B2. Microwave assisted methods and systems for surface decontamination (2024-01-16). Zeteo Tech, Inc. [US]. Inventors: Thomas McCreery [US], Wayne A. Bryden [US].
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Patent 2024
Cells chemical composition Clorox Copper Fungus, Filamentous Ions Microwaves Penicillium roqueforti sodium percarbonate Spores Surface-Active Agents Tween 80
2
Decontamination of Bacillus thuringiensis
Not available on PMC !

Example 1

Thuricide BT Caterpillar Control (Southern Ag) was used as the source of viable Bacillus thuringiensis spores (6 million spores/mg). The DEE chemical comprised of 1:200 bleach in water. This concentration corresponds to approximately 0.5 wt.-% bleach (Clorox) in water. Vegetative cells of BT were grown in lysogeny broth for 48 h at 30° C. The culture was diluted 1:100 with phosphate buffered saline and the DEE chemical composition (about 100 microliters) was added. In the case of spores, the DEE composition was added to achieve a 1:100 dilution of spores.

The spores and vegetative cells in a micro-centrifuge tube were then exposed to 2.45 GHz microwave radiation for 10 s. After exposure, the cells were centrifuged and washed to remove the DEE composition and then plated on Petrifilm and cultured. The plates were then cultured for 24 h at 30° C. This decontamination method resulted in 6-7 log reduction in BT vegetative cells. However, in the case of BT spores, only 4-5 log kill was realized. Increasing the microwave exposure time to 15-20 s yielded 6-7 log kill levels in the case of BT spores.

US11872322B2. Microwave assisted methods and systems for surface decontamination (2024-01-16). Zeteo Tech, Inc. [US]. Inventors: Thomas McCreery [US], Wayne A. Bryden [US].
Full text: Click here
Patent 2024
Bacillus thuringiensis Cells chemical composition Clorox Decontamination Lysogeny Microwaves Phosphates Saline Solution Spores Technique, Dilution Thuricide
3
RF Irradiation Spore Decontamination
Not available on PMC !

Example 5

1:100 dilution of BT spores in DEE chemical formulations were prepared in a 96-well microtiter plate. Three DEE chemical compositions were evaluated: (1) about 0.06 M copper (II) chloride in water, (2) about 1 wt.-% surfactant and 10 wt.-% PCSR in water, and (3) about 1 wt.-% surfactant and about 1 wt.-% PCSR in water. The top of the wells was sealed with a polyolefin sheet. The plates were oriented vertically and were then exposed to 95 GHz RF radiation (centered at 94 GHz) for about 30 s to about 60 s. After RF exposure, the spores were centrifuged, washed to removed DEE chemicals, plated on Petrifilm and cultured. With formulation (2), >6-log kill was realized at both 30 s and 60 s.

US11872322B2. Microwave assisted methods and systems for surface decontamination (2024-01-16). Zeteo Tech, Inc. [US]. Inventors: Thomas McCreery [US], Wayne A. Bryden [US].
Full text: Click here
Patent 2024
chemical composition Chlorides Clorox Copper Microwaves polyolefin Radiation Radiation Exposure Spores Surface-Active Agents Technique, Dilution
4
Comparative Evaluation of Gel Bait Efficacy
Four gel bait products were screened against the JWax-S, I-IN, D-IL, and B-MD strains, as seen in AI-DC assays. Tested gel baits included Maxforce (fipronil), Vendetta (abamectin), Optigard (emamectin benzoate), and Advion (indoxacarb) (Supp Table S2 [online only]). Procedures described previously in other studies were used with minor modifications (Wang et al. 2004 (link), Gondhalekar et al. 2011 (link), Fardisi et al. 2017 (link)). Plastic containers were used (17.8 by 17.8 by 6 cm/0.739 L; Glad boxes Clorox Co., Oakland, CA). The bioassays were conducted in a no-choice format in which no competing food was provided. Polystyrene weighing dishes (Fisher Scientific, Pittsburgh, PA) were filled with 0.5-g gel bait and a water cup, and cardboard shelters were provided in each container. For controls, the gel bait was replaced with a 0.5-g piece of rodent diet. Adult males were starved for one day before assaying. To prevent the cockroaches from escaping, the container walls were lightly greased with petroleum jelly and mineral oil (2:3), and containers were closed tightly with lids containing a central meshed opening (3-cm diameter). Ten replications for each strain–treatment combination were conducted. Mortality was checked every 24 h until 100% mortality was achieved in all strains. All assay boxes were kept 72 h after 100% mortality was achieved to ensure no recovery occurred.
Gits M.P., Gondhalekar A.D, & Scharf M.E. (2023). Impacts of Bioassay Type on Insecticide Resistance Assessment in the German Cockroach (Blattodea: Ectobiidae). Journal of Medical Entomology, 60(2), 356-363.
Publication 2023
abamectin Adult Biological Assay Clorox Cockroaches Diet DNA Replication emamectin benzoate fipronil Food Hyperostosis, Diffuse Idiopathic Skeletal indoxacarb Males Oil, Mineral Petrolatum Polystyrenes Rodent Strains Vision
5
Fungal Pathogen Inoculation of Buxus, Pachysandra, and Sarcococca
Boxwood (Buxus sempervirens ‘Suffruticosa’), pachysandra (Pachysandra terminalis), and sweet box (Sarcococca hookeriana var. humilis) were used. For inoculation, unblemished leaves of similar size and age were collected from healthy specimens of each of these host plants and used after surface sterilization with 10% Clorox® Performance Bleach with CLOROMAX®—Concentrated Formula (Clorox Co., Oakland, CA, USA) for 1 min, rinsing five times with distilled water (dH2O) and then blot-drying with paper towels. The clean, detached leaves, with the abaxial surface facing up, were placed in rows onto polypropylene plastic mesh (Industrialnetting.com, Minneapolis, MN, USA) over wet paper towels in a clear plastic container (31 × 23 × 10 cm, Pioneer Plastics Inc., Eagan, MN, USA) and at this point were ready for inoculation.
Kong P., Daughtrey M.L, & Hong C. (2023). Differential Adaptation Has Resulted in Aggressiveness Variation of Calonectria pseudonaviculata on Hosts Buxus, Pachysandra, and Sarcococca. Journal of Fungi, 9(2), 181.
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Publication 2023
Buxus Clorox Pachysandra Plants Polypropylenes Sarcococca Sterilization Vaccination

Top products related to «Clorox»

1
Tween 20 by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, France, China, Spain, Australia, Japan, India, Poland, Sao Tome and Principe, Switzerland, Macao, Belgium, Canada, Denmark, Israel, Mexico, Netherlands, Singapore, Austria, Ireland, Sweden, Argentina, Romania
Tween 20 is a non-ionic detergent commonly used in biochemical applications. It is a polyoxyethylene sorbitan monolaurate, a surfactant that can be used to solubilize and stabilize proteins and other biomolecules. Tween 20 is widely used in various laboratory techniques, such as Western blotting, ELISA, and immunoprecipitation, to prevent non-specific binding and improve the efficiency of these assays.
2
Clorox by Merck Group
Sourced in Germany
Clorox is a laboratory disinfectant solution primarily used for cleaning and sterilizing equipment and surfaces. It contains sodium hypochlorite as the active ingredient.
3
Triton x 100 by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, China, Japan, France, Canada, Sao Tome and Principe, Switzerland, Macao, Poland, Spain, Australia, India, Belgium, Israel, Sweden, Ireland, Denmark, Brazil, Portugal, Panama, Netherlands, Hungary, Czechia, Austria, Norway, Slovakia, Singapore, Argentina, Mexico, Senegal
Triton X-100 is a non-ionic surfactant commonly used in various laboratory applications. It functions as a detergent and solubilizing agent, facilitating the solubilization and extraction of proteins and other biomolecules from biological samples.
4
Sodium hydroxide (naoh) by Merck Group
Sourced in Germany, United States, India, United Kingdom, Italy, China, Spain, France, Australia, Canada, Poland, Switzerland, Singapore, Belgium, Sao Tome and Principe, Ireland, Sweden, Brazil, Israel, Mexico, Macao, Chile, Japan, Hungary, Malaysia, Denmark, Portugal, Indonesia, Netherlands, Czechia, Finland, Austria, Romania, Pakistan, Cameroon, Egypt, Greece, Bulgaria, Norway, Colombia, New Zealand, Lithuania
Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.
5
Sucrose by Merck Group
Sourced in United States, Germany, United Kingdom, France, Switzerland, Sao Tome and Principe, China, Macao, Italy, Poland, Canada, Spain, India, Australia, Belgium, Japan, Sweden, Israel, Denmark, Austria, Singapore, Ireland, Mexico, Greece, Brazil
Sucrose is a disaccharide composed of glucose and fructose. It is commonly used as a laboratory reagent for various applications, serving as a standard reference substance and control material in analytical procedures.
6
Phytagel by Merck Group
Sourced in United States, Germany, United Kingdom, Japan, Canada, India, Netherlands, Switzerland, France
Phytagel is a natural polysaccharide derived from Sphingomonas paucimobilis bacteria. It is a versatile gelling agent used in various laboratory applications, including cell and tissue culture, microbiology, and biochemistry. Phytagel forms clear, stable gels that can withstand a wide range of pH, temperature, and ionic conditions.
7
Sulfuric acid by Merck Group
Sourced in Germany, United States, Italy, France, India, United Kingdom, Canada, Switzerland, Spain, Australia, Poland, Mexico, Singapore, Malaysia, Chile, Belgium, Ireland, Sweden, Hungary, Brazil, China
Sulfuric acid is a highly corrosive, colorless, and dense liquid chemical compound. It is widely used in various industrial processes and laboratory settings due to its strong oxidizing properties and ability to act as a dehydrating agent.
8
Pd c 10 palladium on c by Merck Group
Pd/C:10%Palladium on C is a heterogeneous catalyst composed of 10% palladium deposited on a carbon support. It is used in various organic synthesis reactions, such as hydrogenation and cross-coupling reactions.
9
Hypercarb cartridge by Thermo Fisher Scientific
The Hypercarb cartridge is a chromatography column product designed for liquid chromatography (LC) applications. It features a porous graphitic carbon stationary phase that can be used for the separation and analysis of a wide range of analytes, including polar, non-polar, and ionizable compounds. The Hypercarb cartridge provides high-performance separations and is compatible with a variety of sample types and mobile phase conditions.

More about "Clorox"

Explore the diverse range of Clorox products, from trusted household cleaners to innovative disinfecting solutions.
This trusted brand has been a staple in homes for over a century, offering effective and convenient cleaning options.
Discover the power of Clorox's bleach-based formulas, including liquid bleach, disinfecting wipes, and cleaning sprays, designed to sanitize surfaces, remove stubborn stains, and kill germs.
Clorox's cleaning prowess extends beyond just household applications.
The company's products are also utilized in various industries, such as healthcare and food service, where sanitiation and disinfection are crucial.
Explore the versatility of Clorox's offerings, including Tween 20, Triton X-100, and Sodium hydroxide, which can be used in laboratory settings for tasks like protein purification and buffer preparation.
Beyond cleaning, Clorox's product portfolio includes other household essentials like Sucrose, Phytagel, and Sulfuric acid, catering to a wide range of consumer needs.
The company's commitment to innovation is evident in its use of advanced ingredients like Pd/C:10%Palladium on C and Hypercarb cartridge, ensuring its products deliver superior performance and effectiveness.
Whether you're a homeowner looking to maintain a clean and healthy living environment or a professional in a specialized industry, Clorox's trusted brand and diverse product lineup can help you achieve your cleaning and disinfecting goals.
Experienece the power of Clorox today and discover the difference it can make in your life.