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Dry Ice

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Most cited protocols related to «Dry Ice»

1
Rapid Golgi Staining of Mouse Brains
Brains were harvested whole from P14 or P25 mice on a 129 background and stained using the FD Rapid GolgiStain kit (FD NeuroTechnologies). Brains were rinsed with double distilled water and then immersed in a 1∶1 mixture of FD Solution A∶B for 2 weeks at room temperature in the dark. Brains were then transferred to FD Solution C and kept in the dark at 4°C for 48 hours. Solution C was replaced after the first 24 hours. In preparation for freezing, individual brains were placed in Peel-A-Way disposable embedding molds (VWR) and immersed in Tissue Freezing Medium (Triangle Biomedical Sciences). Dry ice was used to line the bottom of an ice bucket, which was then filled with 190-proof ethanol (Koptec). Using forceps, the molds were lowered into the ethanol (being careful not to allow the ethanol to spill into the top of the mold) and held until the TFM froze. Brains were kept at −80°C until sectioning. Cryosectioning was performed on a Leica CM 3050 S at −22°C. Coronal sections of 100 µm thickness were cut and transferred to gelatin coated slides (LabScientific) onto small drops of FD Solution C. This thickness enabled optimal staining and preservation of spines on the secondary and tertiary dendritic segments used for analysis in the examples presented here. However, any thickness between 80 to 240 µm (recommended in the FD Rapid GolgiStain instructions) can be used in order to satisfy the user’s unique requirements, providing that individual dendritic spines can still be differentiated and measured. After allowing sections to dry at room temperature in the dark for at least 4 hours (or overnight), slides were then stained exactly as described in the FD Rapid GolgiStain instructions (under “Part VI. Staining Procedure”). Permount (Fisher) was used for coverslipping.
Risher W.C., Ustunkaya T., Singh Alvarado J, & Eroglu C. (2014). Rapid Golgi Analysis Method for Efficient and Unbiased Classification of Dendritic Spines. PLoS ONE, 9(9), e107591.
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Publication 2014
ARID1A protein, human Biologic Preservation Brain Dendrites Dendritic Spines Dry Ice Ethanol Forceps Freezing Fungus, Filamentous Gelatins Mice, 129 Strain Tissues Vertebral Column
2
Whole Mount and Sectioned Embryo Imaging
For whole mount photographs (Figures 2 and 4), unfixed embryos were photographed using a Nikon epifluorescence microscope fitted with Chroma filter sets for ECFP (cyan GFP Ex436/20 Dm455 Bar480/40) and EYFP (yellow GFP Ex500/20 Dm515 Bar535/30). Digital images were acquired using a Spot camera.
For histological sections (Fig 3), embryos were fixed overnight in 4% paraformaldehyde at 4°C, washed 2x for 10 min. in PBS, then equilibrated in the following solutions until the embryos settled at the bottom (approx. 30 min): PBS, 5% sucrose in PBS, 10% sucrose in PBS, and 15% sucrose in PBS. They were then equilibrated in a 1:1 mixture of OCT (Tissue-Tek, Mile, Inc.) and 15% sucrose in PBS for >1 hour, and embedded in OCT over dry ice. Sections were cut at 8 - 12 μM, blow-dried for 30 min. at low heat, then stored at -80°C with desiccant in an air tight bag. Before being photographed, the slides were brought to room temperature, washed 3x in PBS, mounted in Vectashield (Vector Laboratories), covered with a cover glass and sealed with clear nail polish. Sections were photographed as described above.
Srinivas S., Watanabe T., Lin C.S., William C.M., Tanabe Y., Jessell T.M, & Costantini F. (2001). Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Developmental Biology, 1, 4.
Publication 2001
Cloning Vectors Cocaine Cyan 40 Desiccants Dry Ice Embryo Microscopy Nails paraform POU2F1 protein, human Sucrose Tissues
3
Breast Cancer Cohort from Karolinska Hospital
We included all breast cancer patients that were operated on at the Karolinska Hospital from 1 January 1994 to 31 December 1996 (n = 524), identified from the population-based Stockholm–Gotland breast cancer registry established in 1976. Available tumor material was frozen on dry ice or in liquid nitrogen and was stored in -70°C freezers. Figure 1 shows the details of various exclusions leading to the final 159 patients for analysis. The ethical committee at the Karolinska Hospital approved this microarray expression project.
The different reasons for exclusion were not influenced by age at diagnosis (Table 1). The 231 tumors that were not analyzed using expression profiling had a lower mean diameter, had fewer mean affected lymph nodes, and had fewer individuals with recurrent disease at the end of the study period (Table 1). For those excluded for other reasons, there did not seem to be a selection based on age or stage of the disease, compared with those patients included in the study (Table 1).
The Stockholm–Gotland Breast Cancer Registry, supplemented with patient records, were examined for information on the tumor size, the number of retrieved and metastatic axillary lymph nodes, the hormonal receptor status, distant metastases, the site and date of relapse, initial therapy, therapy for possible recurrences, the date and cause of death. Tumor sections from the primary tumors from patients with array profiles were classified using Elston–Ellis grading [18 (link)] by a blinded pathologist (HN).
In the adjuvant setting tamoxifen and/or goserelin is normally used for hormonal treatment, but mostly intravenous cyclophosphamide, methotrexate and 5-fluorouracil (CMF) on days 1 and 8 was used as adjuvant chemotherapy, except in high-risk patients who were offered inclusion in the Scandinavian Breast Group 9401 study [19 (link)]. After primary therapy, patients were recommended to have regular clinical examinations and yearly mammograms, in addition to laboratory and X-ray tests guided by clinical signs and symptoms. Patients were normally followed for 5 years. Patients followed up outside the Karolinska Hospital were tracked using a unique personal identification number. There was no loss to follow-up.
The relapse site, date of relapse, relapse therapy and date of death were ascertained in May 2002. The average follow-up was 6.1 years. Cause of death was coded as death due to breast cancer (including those with distant metastases but dying from other causes), death due to other malignancies and death due to nonmalignant disorders. Through the population-based Swedish Cancer Registry, second primary malignancies were identified.
Pawitan Y., Bjöhle J., Amler L., Borg A.L., Egyhazi S., Hall P., Han X., Holmberg L., Huang F., Klaar S., Liu E.T., Miller L., Nordgren H., Ploner A., Sandelin K., Shaw P.M., Smeds J., Skoog L., Wedrén S, & Bergh J. (2005). Gene expression profiling spares early breast cancer patients from adjuvant therapy: derived and validated in two population-based cohorts. Breast Cancer Research, 7(6), R953-R964.
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Publication 2005
Axilla Breast Chemotherapy, Adjuvant Cyclophosphamide Diagnosis Dry Ice Fluorouracil Freezing Goserelin Malignant Neoplasm of Breast Malignant Neoplasms Mammography Methotrexate Microarray Analysis Neoplasm Metastasis Neoplasms Nitrogen Nodes, Lymph Pathologists Patients Pharmaceutical Adjuvants Physical Examination Precancerous Conditions Radiography Recurrence Relapse Scandinavians Second Primary Cancers Tamoxifen Therapeutics
4
Multimodal Glycemic Monitoring in Clinical Trials
Measures of glycemia included continuous interstitial glucose monitoring (CGM) (CGMS; Medtronic Minimed, Northridge, CA), which measures glucose levels every 5 min and was performed for at least 2 days at baseline and then every 4 weeks during the next 12 weeks. For calibration purposes and as an independent measure of glycemia, subjects performed eight-point (premeal, 90 min postmeal, prebed, and at 3:00 a.m.) self-monitoring of capillary glucose with the HemoCue blood glucose meter (Hemocue Glucose 201 Plus; Hemocue, Ángelholm, Sweden) during the 2 days of CGM. As a third and independent measure of glycemia, subjects were asked to perform seven-point (same as the eight-point profile above without the 3:00 a.m. measurement) fingerstick capillary glucose monitoring (OneTouch Ultra; Lifescan, Milipitas, CA) for at least 3 days per week, at times when CGM was not being performed, for the duration of the study. The results from the CGM and fingerstick monitoring were downloaded from their respective meters and exported to the data coordinating center. To be acceptable for analysis, the CGM data had to include at least one successful 24-h profile out of the 2–3 days of monitoring with no gaps >120 min and a mean absolute difference compared with the Hemocue calibration results <18%, as recommended by the manufacturer.
Blood samples for A1C were obtained at baseline and monthly for 3 months. The blood samples were frozen at −80° C and were sent on dry ice by overnight shipment to the central laboratory. Samples were analyzed with four different DCCT-aligned assays, including a high-performance liquid chromatography assay (Tosoh G7; Tosoh Bioscience, Tokyo, Japan), two immunoassays (Roche A1C and Roche Tina-quant; Roche Diagnostics), and an affinity assay (Primus Ultra-2; Primus Diagnostics, Kansas City, MO). The mean A1C value was used. The laboratory assays were approved by the National Glycohemoglobin Study Program (10 (link)) and have intra- and interassay coefficients of variation <2.5% for low and high values. The assays were highly intercorrelated with R2 values of 0.99 and slopes of ∼1.0 and intercepts between 0.01 and 0.18. Any samples that demonstrated “aging peaks” on high-performance liquid chromatography, evidence of degradation during storage and/or shipment, were considered unacceptable for analysis. One center in Asia was unable to store samples acceptably, resulting in samples that could not be assayed for A1C. The center was eliminated from the study.
Nathan D.M., Kuenen J., Borg R., Zheng H., Schoenfeld D, & Heine R.J. (2008). Translating the A1C Assay Into Estimated Average Glucose Values. Diabetes Care, 31(8), 1473-1478.
Publication 2008
BLOOD Blood Glucose Capillaries Diagnosis Dry Ice Freezing Glucose Hemoglobin, Glycosylated High-Performance Liquid Chromatographies Immunoassay Methamphetamine
5
Affinity Purification of SARS-CoV-2 Protein Interactors
For each affinity purification (26 wild-type and one catalytically dead SARS-CoV-2 baits, one GFP control, one empty vector control), ten million HEK293T/17 cells were plated per 15-cm dish and transfected with up to 15 μg of individual Strep-tagged expression constructs after 20–24 hours. Total plasmid was normalized to 15 μg with empty vector and complexed with PolyJet Transfection Reagent (SignaGen Laboratories) at a 1:3 μg:μl ratio of plasmid to transfection reagent based on manufacturer’s recommendations. After more than 38 hours, cells were dissociated at room temperature using 10 ml Dulbecco’s Phosphate Buffered Saline without calcium and magnesium (D-PBS) supplemented with 10 mM EDTA for at least 5 minutes and subsequently washed with 10 ml D-PBS. Each step was followed by centrifugation at 200 × g, 4°C for 5 minutes. Cell pellets were frozen on dry ice and stored at −80°C. For each bait, n=3 independent biological replicates were prepared for affinity purification.
Gordon D.E., Jang G.M., Bouhaddou M., Xu J., Obernier K., White K.M., O’Meara M.J., Rezelj V.V., Guo J.Z., Swaney D.L., Tummino T.A., Huettenhain R., Kaake R.M., Richards A.L., Tutuncuoglu B., Foussard H., Batra J., Haas K., Modak M., Kim M., Haas P., Polacco B.J., Braberg H., Fabius J.M., Eckhardt M., Soucheray M., Bennett M.J., Cakir M., McGregor M.J., Li Q., Meyer B., Roesch F., Vallet T., Kain A.M., Miorin L., Moreno E., Naing Z.Z., Zhou Y., Peng S., Shi Y., Zhang Z., Shen W., Kirby I.T., Melnyk J.E., Chorba J.S., Lou K., Dai S.A., Barrio-Hernandez I., Memon D., Hernandez-Armenta C., Lyu J., Mathy C.J., Perica T., Pilla K.B., Ganesan S.J., Saltzberg D.J., Rakesh R., Liu X., Rosenthal S.B., Calviello L., Venkataramanan S., Liboy-Lugo J., Lin Y., Huang X.P., Liu Y., Wankowicz S.A., Bohn M., Safari M., Ugur F.S., Koh C., Savar N.S., Tran Q.D., Shengjuler D., Fletcher S.J., O’Neal M.C., Cai Y., C.J.Chang J., Broadhurst D.J., Klippsten S., Sharp P.P., Wenzell N.A., Kuzuoglu D., Wang H.Y., Trenker R., Young J.M., Cavero D.A., Hiatt J., Roth T.L., Rathore U., Subramanian A., Noack J., Hubert M., Stroud R.M., Frankel A.D., Rosenberg O.S., Verba K.A., Agard D.A., Ott M., Emerman M., Jura N., von Zastrow M., Verdin E., Ashworth A., Schwartz O., d’Enfert C., Mukherjee S., Jacobson M., Malik H.S., Fujimori D.G., Ideker T., Craik C.S., Floor S.N., Fraser J.S., Gross J.D., Sali A., Roth B.L., Ruggero D., Taunton J., Kortemme T., Beltrao P., Vignuzzi M., García-Sastre A., Shokat K.M., Shoichet B.K, & Krogan N.J. (2020). A SARS-CoV-2 Protein Interaction Map Reveals Targets for Drug-Repurposing. Nature, 583(7816), 459-468.
Publication 2020
Biopharmaceuticals calcium phosphate Calcium Phosphates Cells Centrifugation Chromatography, Affinity Cloning Vectors Dry Ice Edetic Acid Freezing Hyperostosis, Diffuse Idiopathic Skeletal Magnesium Pellets, Drug Plasmids Saline Solution SARS-CoV-2 Transfection

Most recents protocols related to «Dry Ice»

1
Nucleotide 5'-Phosphoramidate Purification
Not available on PMC !

Example 105

[Figure (not displayed)]

The parent nucleotide 5′-phosphoramidate (0.1 mmol) was suspended in triethylamine (5 mL) and distilled water (5 mL) in a round bottom flask at room temperature. The reaction mixture was stirred at 37° C. for 24 hours, and then the solvents were removed under reduced pressure. The crude residue was purified on silica eluting with 2-propanol, water, ammonia (8:1:1). The fractions containing the desired product were pooled and concentrated under reduced pressure to remove most of the volatiles. The remaining aqueous solution was transferred to a vial, and was then frozen in a dry ice/acetone bath. The material was then freeze-dried to provide the desired product as a white solid.

US11857560B2. Pharmaceutical compositions comprising substituted nucleotides and nucleosides for treating viral infections (2024-01-02). Emory University [US]. Inventors: Dennis C Liotta [US], George R. Painter [US], Gregory R. Bluemling [US], Abel de la Rosa [US].
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Patent 2024
Acetone Ammonia Bath Dry Ice Freezing Isopropyl Alcohol Nucleosides Nucleotides Parent Pharmaceutical Preparations phosphoramidate Pressure Silicon Dioxide Solvents triethylamine Virus Diseases
2
Polyamide 6 Particle Preparation by Melt Emulsification
Not available on PMC !

Example 1

A 1 L stirred reactor from Parr Instruments was used to prepare polyamide 6 particles by melt emulsification. The reactor was loaded with 20 wt % polyamide 6 and 80 wt % 10,000 cSt PDMS oil. The mixture was then heated to 225° C. while stirring at 1000 rpm using a dual 4-blade propeller. After about 60 minutes, the mixture was discharged from the reactor onto dry ice to quench the mixture. The mixture was then filtered and washed to recover the polymer particles. The resultant polymer particles were passed through a 150-μm sieve. Approximately, 40 wt % of the polyamide 6 loaded into the reactor passed through the 150-μm sieve. FIG. 2 is an SEM micrograph of the particles after sieving, which illustrates the range of particle sizes as large.

US11866552B2. Polyamide particles and methods of production and uses thereof (2024-01-09). Xerox Corporation [US]. Inventors: Valerie M. Farrugia [CA], Yulin Wang [CA], Chu Yin Huang [CA], Carolyn Patricia Moorlag [CA].
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Patent 2024
Dry Ice nylon 6 Nylons Polymers
3
Targeted Biogenic Amine Metabolomics
Four ~1.5-g frozen tissue samples collected from specimens 25, 27, 28, and 29 each in 1:1 wt:wt sample to EtOH ratio solutions were shipped on dry ice for biogenic amines panel metabolome analyses by HILIC-QTOF MS/MS [76 (link)] at the UC Davis West Coast Metabolomic Center (http://metabolomics.ucdavis.edu/). Raw output files were curated based on internal standard removal, signal to noise ratio cutoff, and a minimum peak threshold of 1000 and subsequently parsed based on average (n=4) metabolite log fold increases relative to blanks, as suggested elsewhere [77 (link)]. Only metabolites with non-redundant names and InChIKey identifiers and average fold changes higher than 5 relative to blanks were retained for further analyses.
Ramírez G.A., Bar-Shalom R., Furlan A., Romeo R., Gavagnin M., Calabrese G., Garber A.I, & Steindler L. (2023). Bacterial aerobic methane cycling by the marine sponge-associated microbiome. Microbiome, 11, 49.
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Publication 2023
Biogenic Amines Dry Ice Ethanol Freezing Metabolome Tandem Mass Spectrometry Tissues
4
Pancreatic Endocrine Cell Isolation and Sorting
The pancreases from P9-P10 pups were first perfused by 1 mg/ml collagenase in Hank’s balanced salt solution, dissected, and incubated at 37 °C for 10–12 min to release endocrine cells/islets. Digested pancreatic tissue was washed 3 × by 1% FBS in Hank’s solution. To generate single cells, the tissue was further dissociated by trypsinization as described [66 (link)]. Briefly, tissue was dissociated using 0.05% trypsin/0.53 mM EDTA at 37 °C for 5 min. Digestion was stopped by the FACS buffer (2% FBS and 10 mM EGTA in PBS [66 (link)]), and cells were then 1 × washed by FACS buffer. The pancreases microdissected from E14.5 embryos were directly trypsinized and prepared for FACS as described above. Finally, cell suspensions were filtered through 40 µm nylon mesh and immediately tdTomato+ cells were sorted using a flow cytometer (BD FACSAria™ Fusion), through a 100 µm nozzle in 20 psi, operated with BD FACSDiva™ Software (Additional file 1: Fig. S7). For RNA sequencing, 100 sorted cells were collected into individual wells of 96-well plate containing 5 µl of lysis buffer of NEB Next single-cell low input RNA library prep kit for Illumina (New England Biolabs #E6420). Plates were frozen immediately on dry ice and stored at − 80 °C. The total time from euthanasia to cell collection was ∼3 h. For the epigenetic study, on average, 4700 cells/sample at E14.5 and 14,700 cells/sample at P9 were sorted. Cell sorting was performed in the Imaging Methods Core Facility at BIOCEV.
Bohuslavova R., Fabriciova V., Lebrón-Mora L., Malfatti J., Smolik O., Valihrach L., Benesova S., Zucha D., Berkova Z., Saudek F., Evans S.M, & Pavlinkova G. (2023). ISL1 controls pancreatic alpha cell fate and beta cell maturation. Cell & Bioscience, 13, 53.
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Publication 2023
Buffers cDNA Library Cells Collagenase Digestion Dry Ice Edetic Acid Egtazic Acid Embryo Euthanasia Freezing Islets of Langerhans Nylons Pancreas Sodium Chloride System, Endocrine tdTomato Tissues Trypsin
5
Leaf Metabolite Profiling of Populus trichocarpa
Leaves from 851 P. trichocarpa genotypes established in a common garden at Clatskanie, OR in 2009 [20 (link), 21 (link)] were sampled over a 3-day period in July 2012, as described elsewhere [22 (link)], from which 219 samples were selected for inclusion in the present study and were selected as they varied in total and specific aromatic metabolite concentrations based on GC/MS analysis of foliar extracts. Additionally, 60 P. trichocarpa genotypes growing in a drought stress trial at Boardman, OR were sampled on July 11 and 12, 2018, including 2 replicate trees per genotype in a well-irrigated plot (44.7 cm in each of the 2017 and 2018 growing seasons) and 2 replicate trees growing in a drought stress plot receiving 60% (26.8 cm) the irrigation level of the well-irrigated plot. Trees were established in the spring of 2016, with the irrigation manipulation applied during the second (2017) and third (2018) growing seasons. In summary, the Boardman set consisted of 223 P. trichocarpa similar leaf samples collected from trees growing under different irrigation conditions at Boardman, OR in the summer of 2018 in their third year. Similar to the leaves sampled at Clatskanie, OR, a fully expanded leaf of leaf plastochron index 9 ± 1 was rapidly collected, placed on dry ice, shipped back to Oak Ridge National Laboratory, stored at -80ºC until being processed for metabolite analyses.
With Clatskanie, OR, being a mesic site in the Columbia River Delta with a high water table, trees were grown without supplemental irrigation. With Boardman, OR, typically only receiving approx. 18 cm of rainfall annually, trees were supplemented with irrigation water. Surprisingly, there were no significant differences in the total metabolite concentrations between drought-stressed and well-watered trees within a given genotype (data not shown).
Harman-Ware A.E., Martin M.Z., Engle N.L., Doeppke C, & Tschaplinski T.J. (2023). Rapid screening of secondary aromatic metabolites in Populus trichocarpa leaves. Biotechnology for Biofuels and Bioproducts, 16, 41.
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Publication 2023
DNA Replication Droughts Dry Ice Gas Chromatography-Mass Spectrometry Genotype Plant Leaves Rivers Trees

Top products related to «Dry Ice»

1
Trizol reagent by Thermo Fisher Scientific
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
2
Rneasy mini kit by Qiagen
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The RNeasy Mini Kit is a laboratory equipment designed for the purification of total RNA from a variety of sample types, including animal cells, tissues, and other biological materials. The kit utilizes a silica-based membrane technology to selectively bind and isolate RNA molecules, allowing for efficient extraction and recovery of high-quality RNA.
3
Trizol by Thermo Fisher Scientific
Sourced in United States, Germany, China, Japan, United Kingdom, Canada, France, Italy, Australia, Spain, Switzerland, Belgium, Denmark, Netherlands, India, Ireland, Lithuania, Singapore, Sweden, Norway, Austria, Brazil, Argentina, Hungary, Sao Tome and Principe, New Zealand, Hong Kong, Cameroon, Philippines
TRIzol is a monophasic solution of phenol and guanidine isothiocyanate that is used for the isolation of total RNA from various biological samples. It is a reagent designed to facilitate the disruption of cells and the subsequent isolation of RNA.
4
Cryostat by Leica
Sourced in Germany, United States, Japan, United Kingdom, France, Australia, Canada, Denmark, Italy, Singapore, Switzerland, Israel
The Cryostat is a specialized piece of laboratory equipment used for the sectioning of frozen tissue samples. It maintains a controlled low-temperature environment, enabling the precise and consistent cutting of delicate specimens for microscopic analysis and examination.
5
Cm3050 by Leica
Sourced in Germany, United States, Japan, France, United Kingdom, Canada, Switzerland, Austria, Israel
The Leica CM3050S is a cryostat designed for sectioning frozen tissue samples. It features a cooling system that maintains a precise temperature range and enables the creation of high-quality tissue sections. The instrument is engineered to provide consistent and reliable performance for various applications in histology and pathology laboratories.
6
Superfrost plus slide by Thermo Fisher Scientific
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Superfrost Plus slides are high-quality, positively charged microscope slides designed for improved tissue adhesion and cell attachment. These slides feature a specialized coated surface that enhances the binding of biological samples, ensuring secure sample mounting and reliable results during histological and cytological applications.
7
Rnalater by Thermo Fisher Scientific
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RNAlater is a RNA stabilization solution developed by Thermo Fisher Scientific. It is designed to protect RNA from degradation during sample collection, storage, and transportation. RNAlater stabilizes the RNA in tissues and cells, allowing for efficient RNA extraction and analysis.
8
Agilent 2100 bioanalyzer by Agilent Technologies
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The Agilent 2100 Bioanalyzer is a lab instrument that provides automated analysis of DNA, RNA, and protein samples. It uses microfluidic technology to separate and detect these biomolecules with high sensitivity and resolution.
9
Tissue tek oct compound by Sakura Finetek
Sourced in United States, Japan, Netherlands, Germany, France, United Kingdom
Tissue-Tek OCT compound is a tissue-embedding medium designed for cryosectioning. It is formulated to provide optimal support and preservation of tissue samples during the freezing process, enabling the production of high-quality frozen sections for microscopic analysis.
10
Oct compound by Sakura Finetek
Sourced in Japan, United States, Netherlands, Germany, Switzerland, United Kingdom, France
The OCT compound is a specialized laboratory equipment designed for optical coherence tomography (OCT) analysis. It enables high-resolution, non-invasive imaging of biological samples by utilizing low-coherence interferometry. The core function of the OCT compound is to capture detailed, cross-sectional images of various materials and tissues.

More about "Dry Ice"

Dry Ice, also known as solid carbon dioxide (CO2), is a versatile and essential tool in various scientific and industrial applications.
It is widely used for sample preservation, temperature regulation, and cold chain logistics.
Researchers in fields like molecular biology, biochemistry, and cell biology often employ Dry Ice to maintain the integrity and stability of temperature-sensitive samples, such as DNA, RNA, proteins, and cell cultures.
The deep-freezing capabilities of Dry Ice help prevent degradation and ensure reproducible results in experiments.
Techniques like RNA extraction, utilizing reagents like TRIzol and RNeasy Mini Kit, often rely on Dry Ice to chill samples during the process and preserve the delicate RNA molecules.
Similarly, cryosectioning with a cryostat (e.g., CM3050S) and mounting tissue samples on Superfrost Plus slides benefit from the cooling properties of Dry Ice to maintain sample morphology.
Preserving samples in RNAlater solution is another common application of Dry Ice, as it helps stabilize and protect RNA from degradation during transport and storage.
Downstream analyses, such as those performed on the Agilent 2100 Bioanalyzer, can then be conducted on these high-quality samples.
The use of Dry Ice is not limited to the lab; it is also essential in the cold chain logistics of temperature-sensitive materials, including pharmaceutical and biomedical products.
Tissue-Tek OCT compound, for example, is often used in conjunction with Dry Ice to maintain the structural integrity of tissue samples during storage and transportation.
Discover the power of PubCompare.ai, an AI-driven platform that helps researchers effortlessly locate the most effective Dry Ice protocols from research literature, preprints, and patents.
With side-by-side comparisons, you can identify the best Dry Ice methods and products to unlock reproducible research and experiance the future of protocol discovery today.