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Mercuric Chloride

Mercuric Chloride (HgCl2) is an inroganic compound with a wide range of applications in research and industry.
It is commonly used as a disinfectant, preservative, and reagent in various scientific experiments.
This versatile compound has been the subject of extensive study, with researchers investigating its properties, applications, and potential risks.
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Most cited protocols related to «Mercuric Chloride»

1
Phytohormone Analysis by HPLC-MS
Cited 28 times
Cytokinins (zeatin, Z, and zeatin riboside, ZR), indole-3-acetic acid (IAA), and abscisic acid (ABA) were extracted and purified according to the method of Dobrev and Kaminek (2002) (link). One gram of fresh plant material (leaf or root) was homogenized in liquid nitrogen and placed in 5 ml of cold (–20 °C) extraction mixture of methanol/water/formic acid (15/4/1 by vol., pH 2.5). After overnight extraction at –20 °C solids were separated by centrifugation (20 000 g, 15 min) and re-extracted for 30 min in an additional 5 ml of the same extraction solution. Pooled supernatants were passed through a Sep-Pak Plus †C18 cartridge (SepPak Plus, Waters, USA) to remove interfering lipids and plant pigments and evaporated to dryness. The residue was dissolved in 5 ml of 1 M formic acid and loaded on an Oasis MCX mixed mode (cation-exchange and reverse phase) column (150 mg, Waters, USA) preconditioned with 5 ml of methanol followed by 5 ml of 1 M formic acid. To separate different CK forms (nucleotides, bases, ribosides, and glucosides) from IAA and ABA, the column was washed and eluted stepwise with different appropriate solutions indicated in Dobrev and Kaminek (2002) (link). ABA and IAA were analysed in the same fraction. After each solvent was passed through the columns, they were purged briefly with air. Solvents were evaporated at 40 °C under vacuum. Samples then dissolved in a water/acetonitrile/formic acid (94.9:5:0.1 by vol.) mixture for HPLC/MS analysis. Analyses were carried out on a HPLC/MS system consisting of an Agilent 1100 Series HPLC (Agilent Technologies, Santa Clara, CA, USA) equipped with a μ-well plate autosampler and a capillary pump, and connected to an Agilent Ion Trap XCT Plus mass spectrometer (Agilent Technologies, Santa Clara, CA, USA) using an electrospray (ESI) interface. Prior to injection, 100 μl of each fraction extracted from tissues or a similar volume of xylem sap were filtered through 13 mm diameter Millex filters with 0.22 μm pore size nylon membrane (Millipore, Bedford, MA, USA). 8 μl of each sample, dissolved in mobile phase A, was injected onto a Zorbax SB-C18 HPLC column (5 μm, 150×0.5 mm, Agilent Technologies, Santa Clara, CA, USA), maintained at 40 °C, and eluted at a flow rate of 10 μl min−1. Mobile phase A, consisting of water/acetonitrile/formic acid (94.9:5:0.1 by vol.), and mobile phase B, consisting of water/acetonitrile/formic acid (10:89.9:0.1 by vol.), were used for the chromatographic separation. The elution programme maintained 100% A for 5 min, then a linear gradient from 0% to 6% B in 10 min, followed by another linear gradient from 6% to 100% B in 5 min, and finally 100% B maintained for another 5 min. The column was equilibrated with the starting composition of the mobile phase for 30 min before each analytical run. The UV chromatogram was recorded at 280 nm with a DAD module (Agilent Technologies, Santa Clara, CA, USA). The mass spectrometer was operated in the positive mode with a capillary spray voltage of 3500 V, and a scan speed of 22 000 m/z s−1 from 50–500 m/z. The nebulizer gas (He) pressure was set to 30 psi, whereas the drying gas was set to a flow of 6.0 l min−1 at a temperature of 350 °C. Mass spectra were obtained using the DataAnalysis program for LC/MSD Trap Version 3.2 (Bruker Daltonik GmbH, Germany). For quantification of Z, ZR, ABA, and IAA, calibration curves were constructed for each component analysed (0.05, 0.075, 0.1, 0.2, and 0.5 mg l−1) and corrected for 0.1 mg l−1 internal standards: [2H5]trans-zeatin, [2H5]trans-zeatin riboside, [2H6]cis,trans-abscisic acid (Olchemin Ltd, Olomouc, Czech Republic), and [13C6]indole-3-acetic acid (Cambridge Isotope Laboratories Inc., Andover, MA, USA). Recovery percentages ranged between 92% and 95%.
ACC (1-aminocyclopropane-1-carboxylic acid) was determined after conversion into ethylene by gas chromatography using an activated alumina column and a FID detector (Konik, Barcelona, Spain). ACC was extracted with 80% (v/v) ethanol and assayed by degradation with alkaline hypochlorite in the presence of 5 mM HgCl2 (Casas et al., 1989 ). A preliminary purification step was performed by passing the extract through a Dowex 50W-X8, 50–100 mesh, H+-form resin and later recovered with 0.1 N NH4OH. The conversion efficiency of ACC into ethylene was calculated separately by using a replicate sample containing 2.5 nmol of ACC as an internal standard and used for the correction of data.
Albacete A., Ghanem M.E., Martínez-Andújar C., Acosta M., Sánchez-Bravo J., Martínez V., Lutts S., Dodd I.C, & Pérez-Alfocea F. (2008). Hormonal changes in relation to biomass partitioning and shoot growth impairment in salinized tomato (Solanum lycopersicum L.) plants. Journal of Experimental Botany, 59(15), 4119-4131.
Publication 2008
1-aminocyclopropane-1-carboxylic acid Abscisic Acid acetonitrile Capillaries Centrifugation Chaperone-Mediated Autophagy Chromatography cis-acid Cold Temperature CREB3L1 protein, human Cytokinins DNA Replication Dowex Ethanol Ethylenes formic acid Gas Chromatography Glucosides High-Performance Liquid Chromatographies Hypochlorite indoleacetic acid Isotopes Lipids Mass Spectrometry Mercuric Chloride Methanol Nebulizers Nitrogen Nucleotides Nylons Oxide, Aluminum Pigmentation Plant Leaves Plant Roots Plants Pressure Radionuclide Imaging Resins, Plant Sep-Pak C18 Solvents Strains Tissue, Membrane Tissues Vacuum Xylem Zeatin zeatin riboside
2
Structural Analysis of Mutant GltPh Transporter
Cited 14 times
K55C/C321A/A364C mutation was introduced within a heptahistidine mutant of GltPh, used in earlier crystallographic studies8 (link),9 (link), to which we refer as “wild type” for brevity. Purified protein was cross-linked in the presence of 10 fold molar excess of HgCl2, dialyzed against buffer containing 10 mM HEPES/NaOH, 7 mM n-decyl-β-D-maltopyranoside, 100 mM NaCl and 100 μM L-asp, diluted to the final concentration of 2–4 mg/ml and supplemented with 0.5 mM E. coli total polar lipid extract and 100 mM NaBr. Protein solution was mixed at 1:1 (v:v) ratio with the reservoir solution, containing 100 mM MES, pH 5.0, 18–20 % PEG 350 MME and 200 mM CaCl2, and crystallized at 4 °C by hanging drop vapour diffusion. Crystals were cryoprotected by allowing the drop to dry until its volume was reduced by 50 %. Selenomethionine-substituted protein was expressed as described previously8 (link) and crystallized as above. Diffraction data were indexed, integrated and scaled using HKL-2000 package41 . Further analyses were performed using CCP4 programs42 (link). Initial phases were obtained using Phaser43 (link), and the protein model built manually in Coot44 and refined using REFMAC42 (link) with TLS45 (link) and three fold NCS restrains.
Reyes N., Ginter C, & Boudker O. (2009). Transport mechanism of a bacterial homologue of glutamate transporters. Nature, 462(7275), 880-885.
Publication 2009
Buffers Cardiac Arrest Crystallography Diffusion Escherichia coli HEPES Lipids Mercuric Chloride Molar Mutation Proteins Selenomethionine Sodium Chloride
3
Purification and Cross-linking of Glt(Ph) Transporter
Cited 12 times
GltPh-K55C/C321A/A364C was expressed as His8 fusion and purified as described previously8 (link). Transporter samples were exchanged by size exclusion chromatography (SEC) into buffer, containing (in mM) 10 HEPES/NaOH or KOH, pH 7.4, 1 n-dodecyl-β-D-maltopyranoside and either 100 NaCl and 0.1 L-asp or 100 KCl. Cross-linking was initiated by addition of 1:2 molar ratio of Cu2+ and 1,10 phenantroline or HgCl2. Reactions were quenched with 100 mM N-ethyl maleimide prior to SDS PAGE analysis. Crude E. coli membranes were isolated by centrifugation, washed either in a Na+/L-asp-containing or free buffer and cross-linked as in detergent. Protein bands were visualized by western blotting using antibodies against histidine tag.
Reyes N., Ginter C, & Boudker O. (2009). Transport mechanism of a bacterial homologue of glutamate transporters. Nature, 462(7275), 880-885.
Publication 2009
Antibodies aspartic acid beta-naphthylamide Buffers Centrifugation Detergents Escherichia coli Gel Chromatography HEPES Histidine maleimide Membrane Transport Proteins Mercuric Chloride Molar Proteins SDS-PAGE Sodium Chloride Tissue, Membrane
4
Generating Transgenic Brassica napus via Agrobacterium-Mediated Transformation
Cited 7 times
The 35S::BraLTP1 fragment in PBI121s was introduced into Agrobacterium tumefaciens GV3101 by electroporation, and positive clones were selected on on LB agar plates at 37°C, supplemented with appropriate concentration of antibiotics (gentamicin 50 mg L−1, rifampicin 50 mg L−1 and kanamycin 50 mg L−1) and PCR verified. A single positive colony was used to transform B. napus cv. Zhongshuang 6, an elite Chinese cultivar in China, as follows: Seeds of Zhongshuang 6 were soaked in 75% ethanol for 1 min and for 10–15 min in a 1.5% mercuric chloride solution. Five to six days after germination under darkness, etiolated hypocotyls were cut in 7 mm segments and mixed with 50 mL Agrobacterium in liquid DM media (MS+30 g L−1 sucrose+100 µM acetosyringone, pH 5.8) (OD ∼0.3) for 0.5 h. Surface air dried hypocotyls were then transferred to co-cultured medium (MS+30 g L−1 sucrose+18 g L−1 manitol+1 mg L−1 2, 4-D+0.3 mg L−1 kinetin+100 µM acetosyringone+8.5 g agrose, pH 5.8) for 2 days and then to a selection medium (MS+30 g L−1 sucrose+18 g L−1 manitol+1 mg L−1 2, 4-D+0.3 mg L−1 kinetin+20 mg L−1 AgNO3+8.5 g L−1 agrose+25 mg L−1 kanamycin+250 mg L−1 carbenicillin pH 5.8) for proliferation. After 3 weeks, hypocotyl callus was transferred to regeneration medium (MS+10 g L−1 glucose+0.25 g L−1 xylose+0.6 g L−1 MES hydrate+2 mg L−1 zeatin+0.1 mg L−1 indole-3-acetic acid+8.5 g L−1 agrose+25 mg L−1 kanamycin+250 mg L−1 carbenicillin, pH 5.8) for 2 weeks. Hypocotyls were transferred to new regeneration media every 2 weeks for 3∼4 regeneration cycles before transfer to radication medium (MS+10 g L−1 sucrose+10 g L−1 agar, pH 5.8) for rooting (about 3 weeks). Transformed plants with roots were transplanted into pots and grown as described. For the construct, more than 60 independent 35S::BraLTP1 T0 transgenic plants were generated, and more than 85% were positive transformants as detected using a forward primer designed to the CaMV 35S sequence (35S-F: 5′-AGGACACGCTGAAATCACCA-3) and a reverse primer designed to BraLTP1 (D-BraLTP1-R: 5′-GGATCCCAAACCTCATGGCACAATGTA-3′). T1 seeds of PCR-positive transformants were harvested and grown to T2 generation for phenotype identification.
Liu F., Xiong X., Wu L., Fu D., Hayward A., Zeng X., Cao Y., Wu Y., Li Y, & Wu G. (2014). BraLTP1, a Lipid Transfer Protein Gene Involved in Epicuticular Wax Deposition, Cell Proliferation and Flower Development in Brassica napus. PLoS ONE, 9(10), e110272.
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Publication 2014
acetosyringone Agar Agrobacterium Agrobacterium tumefaciens Antibiotics, Antitubercular Callosities Carbenicillin Chinese Clone Cells Darkness Electroporation Ethanol Gentamicin Germination Glucose Hypocotyl indoleacetic acid Kanamycin Kinetin Mannitol Marijuana Abuse Mercuric Chloride MS 1-2 Oligonucleotide Primers Phenotype Plant Embryos Plant Roots Plants, Transgenic Regeneration Rifampin Sucrose Xylose Zeatin
5
Synchronized Toxoplasma tachyzoite culture
Cited 7 times
T. gondii tachyzoites (RH strain) were grown in h-Tert human fibroblasts (Farwell et al., 2000 (link)) as described before (Moreno & Zhong, 1996 (link)). These cells grow in DMEM media containing 1% FBS.
For semisynchronization of cultures, h-Tert cells cultured in 75 cm2 flasks, were infected with 3.7 × 107 tachyzoites/flask for two hours, extracellular parasites thoroughly washed and the cultures allowed to grow for 35–40 hours. At this time, extracellular parasites were removed by washing with fresh invasion medium (IM) (DMEM containing 20 mM Hepes pH 7.4 with 1% FBS) 3 times and the cultures allowed growth for two more hours in IM. Subsequently, the extracellular tachyzoites were washed off and the intracellular tachyzoites collected in fresh IM by scrapping off the host monolayer and purifying the parasites by filtration through a nucleopore membrane. The isolated tachyzoites were centrifuged and resuspended in IM without serum or Buffer A plus glucose (BAG) (116 mM NaCl, 5.4 mM KCl, 0.8 mM MgSO4, 50 mM Hepes, pH 7.2, 5.5 mM glucose) at a concentration of 5 × 107 tachyzoites/ml, and incubated for two or four hours at 37°C.
For stress experiments involving mercury tolerance, tachyzoites were incubated in BAG with 1 μM HgCl2 for 5 min, fixed with 4% paraformaldehyde and mounted on coverslips. Using Image J software (NIH), circularity measurements (a metric of roundness where 1 equals a perfect circle) were made on 50 randomly chosen cells from each treatment to determine changes in overall cell shape when in the presence or absence of 1 μM HgCl2.
For salt stress experiments freshly egressed tachyzoites were purified and washed in IM, incubated for 15 and 30 min under stress conditions (described in the legend for Fig. 7) and subsequently added to the regular culture medium. For plaque assays confluent monolayers of fibroblasts grown in 6 well plates were infected, in triplicate, with 225 tachyzoites per well. The parasites were allowed to plaque for 9 days, fixed, and stained as described (Roos et al., 1994 (link)).
Miranda K., Pace D.A., Cintron R., Rodrigues J.C., Fang J., Smith A., Rohloff P., Coelho E., de Haas F., de Souza W., Coppens I., Sibley L.D, & Moreno S.N. (2010). Characterization of a novel organelle in Toxoplasma gondii with similar composition and function to the plant vacuole. Molecular microbiology, 76(6), 1358-1375.
Publication 2010
Biological Assay Buffers Cells Cell Shape Culture Media Dental Plaque Fibroblasts Filtration Glucose HEPES Homo sapiens Immune Tolerance Mercuric Chloride Mercury Nuclear Pore paraform Parasites Protoplasm Salt Stress Serum Sodium Chloride Strains Stress Disorders, Traumatic Sulfate, Magnesium TERT protein, human Tissue, Membrane

Most recents protocols related to «Mercuric Chloride»

1
Heavy Metal Tolerance of B. licheniformis
Not available on PMC !

Example 2

This example illustrates the heavy metal acceptability of B. licheniformis strain ECOBIO_2.

The pure cultures of each isolates were grown in LB broth and the bacterial cell concentration was spectrophotometrically adjusted to have an OD of 1.0 at A600 nm. 1 ml of cells were inoculated to 100 ml of sterilize LB medium amended with either 1 mM Pb(C2H3O2)2, CdCl2, K2Cr2O7 or with 0.3 mM HgCl2 in individual culture flasks. Flasks were incubated at 37° C. and 150 rpm. The growth rate was measured by assessing the turbidity of the medium every 24 hours after initial incubation for 3 days.

Data obtained from three independent experiments performed to determine the heavy metal acceptability of strain ECOBIO_2. Results (shown in FIG. 3) demonstrated that B. licheniformis strain ECOBIO_2 could grow in the presence of lead similar to controls (LB alone). This signifies the noteworthy characteristics of bacteria towards tolerating Pb2+ toxicity. However, the growth of B. licheniformis strain ECOBIO_2 was significantly reduced in the presence of cadmium, chromium, and mercury with mercury being the most toxic metal among the tested heavy metals.

US11865597B2. Method for bioremediation of lead (2024-01-09). None [IN], None [US], None [JP], None [IN]. Inventors: Sunil Kumar Channarayapatna Ramesh [IN], Emmanuel Stephen Victor [US], Kishore Babu Naripogu [JP], Geetha Nagaraja [IN].
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Patent 2024
Bacteria Bioremediation Cadmium Cells Chloride, Cadmium Chromium Heavy Metal Poisonings Mercuric Chloride Mercury Metals, Heavy Potassium Dichromate Sterilization Strains
2
Isolation and Cultivation of Cordyceps sinensis Ascospores
Cited 2 times
Immature C. sinensis specimens were purchased from local markets from the Hualong (located at 36°13’N, 102°19’E) and Yushu areas (located at 33°01’N, 96°48’E) of Qinghai Province of China (3800–4600 m’ altitude) in mid-May and characterized by a plump caterpillar body and very short stroma (1.0–2.0 cm) [19 , 27 ]. Mature C. sinensis specimens were collected in mid-June and characterized by a plump caterpillar body and long stroma (>5.0 cm) and by the formation of an expanded fertile portion close to the stromal tip, which was densely covered with ascocarps (Fig 1). Governmental permission was not required for C. sinensis purchases in local markets, and the collections of C. sinensis specimens from sales by local farmers fall under the governmental regulations for traditional Chinese herbal products.
The specimens were washed thoroughly on site in running water with gentle brushing, soaked in 0.1% mercuric chloride for 10 min for surface sterilization and washed 3 times with sterile water. The thoroughly cleaned specimens were immediately frozen in liquid nitrogen on site and kept frozen during transportation to the laboratory and during storage prior to further processing [19 , 27 ].
Some of the mature C. sinensis specimens were harvested along with the outer mycelial cortices and soil surrounding the caterpillar body and replanted in paper cups in soil obtained from C. sinensis production areas (Fig 1A) and were cultivated in our laboratory (altitude 2,200 m) in Xining City, Qinghai Province of China [67 , 68 ]. Because of the phototropism of natural C. sinensis, we kept the windows fully open, allowing sufficient sunshine and a natural plateau breeze blowing over the cultivated specimens in the paper cups. The room temperature was maintained naturally, fluctuating with the lowest temperature at 18–19°C during the night and the highest temperature at 22–23°C in the early afternoon. The humidity of our laboratory was maintained by spraying of water using an atomizer twice a day in the morning and evening.
Fully ejected ascospores of C. sinensis were collected using double layers of autoclaved weighing paper (Fig 1B). During massive ascospore ejection, numerous ascospores adhered to the outer surface of asci, as shown in Fig 1C after removing the upper layer of autoclaved weighing papers for collection of the fully ejected ascospores, and failed to be brushed away using an autoclaved brush; hence, these ascospores were instead gently scratched off using a disinfected inoculation shovel or ring and referred to as semiejected ascospores.
The 2 types of ascospores were cleaned by 2 washes with 10% and 20% sucrose solutions and 10-min centrifugation at 1,000 rpm (desktop centrifuge, Eppendorf, Germany); the supernatant was discarded after each centrifugation. The pellets (ascospores) were subsequently washed with 50% sucrose solution and centrifuged for 30 min, and the ascospores that floated on the liquid were collected [67 ]. The fully and semiejected ascospores were stored in a -80°C freezer prior to further processing.
Li Y.L., Li X.Z., Yao Y.S., Wu Z.M., Gao L., Tan N.Z., Luo Z.Q., Xie W.D., Wu J.Y, & Zhu J.S. (2023). Differential coexistence of multiple genotypes of Ophiocordyceps sinensis in the stromata, ascocarps and ascospores of natural Cordyceps sinensis. PLOS ONE, 18(3), e0270776.
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Publication 2023
Atomizers Centrifugation Chinese Cold Temperature Cortex, Cerebral Farmers Fertility Fever Freezing Human Body Humidity Mercuric Chloride Mycelium Nitrogen Pellets, Drug Phototropism Specimen Collection Sterility, Reproductive Sterilization Sucrose Sunlight Vaccination
3
Molecular Characterization of C. sinensis
Laboratory common reagents such as ethanol, sucrose, paraformaldehyde, hematoxylin, eosin, agarose and electrophoresis reagents, etc. were purchased from Beijing Bioland Technology Company. Mercuric chloride (0.1%) for surface sterilization of freshly collected C. sinensis specimens was a gift from the Institute of Microbiology, Chinese Academy of Sciences. EcoRI endonuclease is a product of New England BioLabs, United States. The Universal DNA Purification kit was a product of TIANGEN BIOTECH Company, China. The DNeasy Plant Mini Kit was a product of Qiagen Company, Germany. The Gel Extraction Kit was a product of Omega Bio-Tek, United States. The Taq PCR reagent kit and Vector NTI Advance 9 software were purchased from Invitrogen, United States. Calmodulin was a product of Abicom (Shanghai, China).
Li Y.L., Li X.Z., Yao Y.S., Wu Z.M., Gao L., Tan N.Z., Luo Z.Q., Xie W.D., Wu J.Y, & Zhu J.S. (2023). Differential coexistence of multiple genotypes of Ophiocordyceps sinensis in the stromata, ascocarps and ascospores of natural Cordyceps sinensis. PLOS ONE, 18(3), e0270776.
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Publication 2023
Calmodulin Chinese Cloning Vectors Deoxyribonuclease EcoRI Electrophoresis Eosin Ethanol Hematoxylin Mercuric Chloride paraform Plants Sepharose Sterilization Sucrose
4
Inoculation of Common Bean Varieties
We carried out an experiment in a screen house to determine the mineralization potential and phenotypic characteristics of selected isolates. Common bean varieties from Kenya Seed Company (Rose coco and Mwitemania) were used as test crops for total plant biomass. This was carried out by inoculating two high-potential PSB (KB5 and KV1) into two varieties of common bean to determine their efficacy. Certified bean seeds were surface sterilized with 1% mercuric chloride for 3 minutes followed by rinsing with distilled water and pregermination in a darkroom using Petri dishes. Inoculants were prepared according to [26 (link)]. The isolates were grown in NBRIB broth for 2 days and cells were harvested by centrifugation at 5000 × g for 20 min. The cells were resuspended with sterile distilled water to give a final concentration (108 CFU ml−1) in a 250 mL conical flask. The seedlings' roots were immersed into the culture for 5 minutes and covered uniformly with a 15 mm thick layer of vermiculite in a Leonard's Jar and then placed into a completely randomized design alongside negative control (uninoculated seedlings). A total of six treatments were replicated four times to obtain 24 experimental units with two trials. Leonard's jars assemblies [37 (link)] (9 cm diameter and 12 cm height) were filled with the sterile vermiculite (Kenworks, Nairobi, Kenya). Tricalcium phosphate was provided as soil inorganic phosphorus fertilizer at the rate of 150 mg/kg based on the nutrient necessities of common bean plants [38 (link)]. Depth (5 cm) was dug into Leonard's Jar, and two seedlings were placed at equal distances. A modified nutrient solution without phosphorus was supplied to all treatments [39 ]. After 6 weeks, plant samples were uprooted and oven-dried at 70°C to a constant weight and were grinded after drying to determine the total dry weight in grams.
Kiprotich K., Muoma J., Omayio D.O., Ndombi T.S, & Wekesa C. (2023). Molecular Characterization and Mineralizing Potential of Phosphorus Solubilizing Bacteria Colonizing Common Bean (Phaseolus vulgaris L.) Rhizosphere in Western Kenya. International Journal of Microbiology, 2023, 6668097.
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Publication 2023
Agricultural Crops Cacao Cells Centrifugation Hyperostosis, Diffuse Idiopathic Skeletal Mercuric Chloride Nutrients Phaseolus vulgaris Phenotype Phosphorus Physiologic Calcification Plant Embryos Plant Roots Plants Seedlings Sterility, Reproductive tricalcium phosphate vermiculite
5
Sediment Core Slurry Incubation with 15N and 35S
PVC core liners (I.D. 3.5 cm) were used to collect three vertical cores from the upper sand flat of Janssand during low tide on two occasions (October 17, 2018 and May 22, 2019) and transported to the lab (~2 h). In October surface water was approximately 14 °C, and in May 11 °C.
Cores were transferred to an anaerobic chamber and the upper pale (oxidized) layer (0–3 cm) was separated from a dark (reduced) layer (7–10 cm) (Supplementary Fig. 1). The upper layer was well mixed before 2 cm3 aliquots of sediment was transferred into 12 mL glass vials with septa (LabCo, Manchester), hereafter referred to as “Exetainers”, that were filled with filtered anoxic seawater collected October 10, 2018 (NO3- + NO2-- < 2 µM) creating sediment slurries. Exetainers were capped headspace free and removed from the anaerobic chamber whereupon they were assigned to one of three treatment groups (Supplementary Fig. 1). 38 Exetainers per core received 60 µM 15N-labeled NO3- (corresponding to ~300 nmol/cm3 sediment), 24 received 60 µM 15N-labeled NO3- and 250 kBq of 35S-labeled sulfate, 24 received only 250 kBq of 35S-labeled sulfate. Filled Exetainers were placed in roller tanks on a roller table. The roller table speed was set in order to gently invert the Exetainers every 44 seconds along their longitudinal axis to ensure that the slurries remained homogenous. Visual observations confirmed that this constantly mixed the sediment with the seawater in the vials.
Slurries were weighed and killed in duplicates at 12 selected time points with the aim of including timepoints before and after NO3- depletion. Slurries without added 35S (i.e. those with only 15N) were killed by injecting 100 µL 30% w/v zinc chloride and 200 µL saturated mercury chloride so that they were suitable for later 15N gas analysis. Slurries with added 35S were killed by first removing 1.8 mL sample water that was directly pipetted into 200 µL 20% w/v zinc acetate (total radioactivity samples) and stored at 4 °C, and the remaining sediment and water was decanted directly into 50 mL falcon tubes pre-filled with 7 mL 30% zinc acetate (TRIS samples) and frozen at −20 °C.
Bourceau O.M., Ferdelman T., Lavik G., Mussmann M., Kuypers M.M, & Marchant H.K. (2023). Simultaneous sulfate and nitrate reduction in coastal sediments. ISME Communications, 3, 17.
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Publication 2023
Anoxia Dental Cavity Liner Epistropheus Freezing Homozygote Mercuric Chloride Neoplasm Metastasis Radioactivity Sulfates, Inorganic Tromethamine Zinc Acetate zinc chloride

Top products related to «Mercuric Chloride»

1
Hgcl2 by Merck Group
Sourced in United States, Germany, United Kingdom, India
HgCl2, also known as mercury(II) chloride, is a chemical compound commonly used in laboratory settings. It is a white, crystalline solid that is soluble in water and organic solvents. HgCl2 is primarily utilized as a reagent in various analytical and synthetic procedures conducted in research and development laboratories.
2
Mercuric chloride by Merck Group
Sourced in United States, Germany, India
Mercuric chloride is a chemical compound with the formula HgCl2. It is a white, crystalline solid that is commonly used as a laboratory reagent.
3
Hgcl2 by Sinopharm
Sourced in China
HgCl2 is a chemical compound consisting of mercury and chlorine. It is a white crystalline solid that is commonly used as a laboratory reagent.
4
Sodium chloride (nacl) by Merck Group
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NaCl is a chemical compound commonly known as sodium chloride. It is a white, crystalline solid that is widely used in various industries, including pharmaceutical and laboratory settings. NaCl's core function is to serve as a basic, inorganic salt that can be used for a variety of applications in the lab environment.
5
Sodium hydroxide (naoh) by Merck Group
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Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.
6
Zncl2 by Merck Group
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ZnCl2 is a chemical compound that can be used as a laboratory reagent. It is a white crystalline solid that is soluble in water and other polar solvents. ZnCl2 is commonly used in various chemical and analytical applications, but a detailed description of its core function is not available without the risk of unintended interpretation or extrapolation.
7
Hydrochloric acid (hcl) by Merck Group
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Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.
8
Mercury chloride by Merck Group
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Mercury chloride is a chemical compound with the formula HgCl2. It is a white, crystalline solid that is used in various laboratory applications. Mercury chloride has a high boiling point and is soluble in water.
9
Cdcl2 by Merck Group
Sourced in United States, Germany, Italy, United Kingdom, China, Sao Tome and Principe
CdCl2 is a chemical compound that is commonly used in various laboratory applications. It is a white, crystalline solid that is soluble in water and various organic solvents. CdCl2 is primarily used as a precursor for the synthesis of other cadmium-containing compounds, as well as in thin-film deposition processes.
10
Potassium chloride (kcl) by Merck Group
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Potassium chloride (KCl) is an inorganic compound that is commonly used as a laboratory reagent. It is a colorless, crystalline solid with a high melting point. KCl is a popular electrolyte and is used in various laboratory applications.

More about "Mercuric Chloride"

Mercuric Chloride (HgCl2), also known as mercury(II) chloride or corrosive sublimate, is a versatile inorganic compound with a wide range of applications in research and industry.
This versatile substance is commonly used as a disinfectant, preservative, and reagent in various scientific experiments and industrial processes.
Mercuric Chloride has been the subject of extensive study, with researchers investigating its properties, applications, and potential risks.
The compound's unique chemical characteristics, such as its high density, thermal stability, and reactivity, make it a valuable tool in a variety of fields, including analytical chemistry, materials science, and environmental remediation.
In addition to its primary uses, Mercuric Chloride is related to other inorganic compounds like Sodium Chloride (NaCl), Sodium Hydroxide (NaOH), Zinc Chloride (ZnCl2), and Hydrochloric Acid (HCl).
These compounds share similarities in their chemical structures and can be used in conjunction with Mercuric Chloride for various applications.
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OtherTerms: HgCl2, Mercury chloride, Mercuric chloride, CdCl2, KCl