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> Chemicals & Drugs > Inorganic Chemical > Orthophosphate

Orthophosphate

Orthophosphates are a class of phosphate compounds that are widely used in various scientific and industrial applications.
These compounds are characterized by the presence of the orthophosphate (PO4^3-) ion, which consists of a central phosphorus atom bonded to four oxygen atoms.
Orthophosphates play a crucial role in many biological processes, including energy metabolism, signal transduction, and structural components of cells.
They are also commonly used in detergents, fertilizers, and water treatment applications.
This MeSH term provides a comprehensive overview of the properties, functions, and applications of orthophosphates, offering researchers and professionals a concise and informative resource for their work.

Most cited protocols related to «Orthophosphate»

1
RNA Extraction and Northern Blot Analysis
Cited 9 times
RNA was extracted from cell pellets using TRI reagent (Sigma-Aldrich) and analyzed by glyoxal-agarose gel or urea-PAGE and transferred to nylon membrane. For Northern blot analysis, oligonucleotides (Table S4) were 5′ labeled with γ-[32P]ATP using T4 polynucleotide kinase and used as probes. Random prime-labeled probes hybridizing immediately upstream and downstream of the A’ cleavage in the 5′ETS (ETS1 and ETS2, respectively) were produced from PCR products (Turner et al., 2009 (link)). Random prime-labeled probes against the full-length RNase MRP RNA and the S domain of 7SL were also generated.
For metabolic labeling experiments using 3H methyl-methionine, the cells were incubated in methionine-free media (15 min) and then in methionine-free media containing 50 µCi/ml 3H-labeled methyl-methionine (30 min). The cells were then incubated in normal media containing 10× the normal concentration of methionine and harvested at the required time points (0, 15, 30, 60, 120, and 240 min). For metabolic labeling experiments using 32P orthophosphate, cells were grown in phosphate-free media (1 h) followed by phosphate-free media containing 15 µCi/ml 32P-labeled inorganic phosphate (1 h). Cells were then incubated in normal media and harvested at the required time points (0, 30, 60, 120, and 240 min). RNA was extracted using TRI reagent and analyzed by agarose-glyoxal or urea-PAGE as appropriate. Results were visualized using a phosphorimager (Typhoon FLA9000; GE Healthcare). All quantitation was normalized to the levels of mature 18S or 28S rRNA, as appropriate.
Sloan K.E., Mattijssen S., Lebaron S., Tollervey D., Pruijn G.J, & Watkins N.J. (2013). Both endonucleolytic and exonucleolytic cleavage mediate ITS1 removal during human ribosomal RNA processing. The Journal of Cell Biology, 200(5), 577-588.
Publication 2013
Cells Cytokinesis Glyoxal Methionine methionine methyl ester Northern Blotting Nylons Oligonucleotides Orthophosphate Pellets, Drug Phosphates Polynucleotide 5'-Hydroxyl-Kinase RNA, Ribosomal, 28S RNA Probes RNAse MRP Sepharose Tissue, Membrane Typhoons Urea
2
Metabolic Connectivity Mapping in Yeast
Cited 9 times
Metabolism was represented in a form of a connectivity graph. The nodes of the graph correspond to metabolic genes, and edges correspond to connections established by metabolic reactions. Metabolic genes X and Y are considered connected if and only if there exists a metabolite that is present among the list of either reactants or products of reactions catalyzed by enzymes encoded by both X and Y.
The metabolic connectivity graph is used to calculate network distance (or metabolic separation) between genes. We define a pair of directly connected metabolic genes as being separated by distance 1. In general, we define network distance between the genes X and Y as the length of the shortest path from X to Y on the metabolic connectivity graph. A hand-curated metabolic network model of S. cerevisiae (Forster et al, 2003 (link)) was used to construct a comprehensive metabolic connectivity graph. While any metabolite can be used to deduce gene connectivity, the relationships established by the common cofactors, such as ATP, are not likely to connect genes with similar metabolic functions. In compiling a global metabolic connectivity graph, we consider a subset of metabolites, which excludes most highly connected metabolic species. An exclusion threshold was determined based on the connectivity of the resulting gene dependency graph (Supplementary Figure 1). A total of 14 most highly connected metabolites (ATP, ADP, AMP, CO2, CoA, glutamate, H, NAD, NADH, NADP, NADPH, NH3, orthophosphate, pyrophosphate) and their mitochondrial and external analogs were excluded. The general trends described in the paper are not sensitive to the precise choice of the metabolite set; however, the actual values change when more or less metabolites are considered. For detailed analysis, see Supplementary information. Genes encoding enzymes that are part of known complexes, according to MIPS complex database (http://mips.gsf.de) and SGD (http://www.yeastgenome.org/), were masked as unassigned enzymes, so that their expression profiles would not be included in any of the analysis (36 enzyme-encoding genes in total).
Kharchenko P., Church G.M, & Vitkup D. (2005). Expression dynamics of a cellular metabolic network. Molecular Systems Biology, 1, 2005.0016.
Publication 2005
Diphosphates Enzymes Gene Regulatory Networks Genes Glutamate Macrophage Inflammatory Protein-1 Metabolic Networks Metabolism Mitochondria NADH NADP Operator, Genetic Orthophosphate
3
Phospholipid Labeling and Analysis
Cited 7 times
Five-days-old seedlings or leaf disks (5 mm ∅) of 3-weeks-old plants were transferred to a 2.0 ml Eppendorf tube, containing MES (2-[N-morpholino]ethane sulfonic acid)-based buffer of 2.56 mM MES (pH 5.7) and 1 mM KCl. To label phospholipids, 10 μCi carrier-free 32P-orthophosphate per tube was added for 16 h, unless indicated otherwise. Cold shock treatments were executed by transferring tubes to ice water. Incubations were stopped by the addition of HClO4 (final concentration 5%, w/v), and 10 min of subsequent shaking.
The total solvent was removed and 375 μl CHCl3/MeOH/HCl (50:100:1, by vol.) was added to extract the lipids. After 10 min of vigourous shaking, two phases were induced by adding 375 μl CHCl3 and 200 μl 0.9% (w/v) NaCl. The organic lower phase was then transferred to a tube containing 375 μl CHCl3/MeOH/1M HCl (3:48:47, by vol.). Shaking, spinning, and removing the upper phase yielded a purified organic phase, which was dried down in a vacuum centrifuge at 50°C. The residue was resuspended in 50 μl CHCl3 and sampled for lipid analysis.
Phospholipids were analyzed by thin-layer chromatography (TLC) on heat-activated silica gel 60 plates (Merck, 20 × 20 cm) using one of the following solvent systems (ratios by vol.): (A) CHCl3/MeOH/NH4OH (25%)/H2O (90:70:4:16); or (B) ethylacetate/iso-octane/formic acid/H2O (13:2:3:10), of which the organic phase was used for TLC. Solvent A was used for total phospholipid analysis, while B was used to quantitate PtdOH and PtdBut. Radiolabeled phospholipids were visualized and quantified by phosphoimaging (Molecular Dynamics, Sunnyvale CA, USA).
Arisz S.A., van Wijk R., Roels W., Zhu J.K., Haring M.A, & Munnik T. (2013). Rapid phosphatidic acid accumulation in response to low temperature stress in Arabidopsis is generated through diacylglycerol kinase. Frontiers in Plant Science, 4, 1.
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Publication 2013
2,2,4-trimethylpentane Buffers Chloroform Cold Shock Stress Cold Temperature Electroconvulsive Therapy ethane sulfonate ethyl acetate formic acid Lipids Molecular Dynamics Morpholinos Orthophosphate phosphatidylbutanol Phospholipids Plant Leaves Plants Seedlings Silica Gel Sodium Chloride Solvents Thin Layer Chromatography Vacuum
4
Radioactive Labeling and Immunoprecipitation of Proteins
Cited 5 times
Radioactive labeling of cells was performed on subconfluent cultured cells grown on 90-mm plates. For the 33P-labeling, A431 cells were first incubated for 1 h at 37°C in serum-free and phosphate-free medium (Sigma Chemical Co.) before adding 0.5 mCi of 33P-orthophosphate (Amersham, UK) to each 90-mm plate. After further incubation for 1 h at 37°C, cells were stimulated with 200 ng/ml EGF for different times up to 15 min. Cells were then quickly washed with TBS and scraped into 1 ml of extraction buffer containing phosphatase and protease inhibitors: 25 mM Tris-HCl, pH 7.4, containing 150 mM NaCl, 1 mM EDTA, 0.1% SDS, 1% Triton X-100, 1% deoxycholate, 1 mM PMSF, a protease inhibitor cocktail (complete™, Boehringer), 1 mM Na3VO4, 30 mM NaF, and 10 mM Na2P2O7. Similarly for 35S-labeling, A431 and NRK cells grown on 90-mm plates were preincubated for 1 h at 37°C in methionine/cysteine-free MEM containing 5% dialysed FCS. After a brief wash with PBS, 0.2 mCi of [35S]methionine/cysteine (Pro-mix; Amersham, UK) in 4 ml of methionine/cysteine free MEM supplemented with 20 mM Hepes, pH 7.4, was added to the cells and incubation continued for 1 h at 37°C. The cells were washed three times with PBS and scraped into 1 ml of extraction buffer without the phosphatase inhibitors. The cell extracts after 35S- or 33P-labeling were first sonicated on ice to reduce viscosity and then cleared by centrifugation at 50,000 g for 30 min. 200 μl of supernatant was mixed first with 20 μl of nonimmune rabbit serum and 20 μl of protein A–Sepharose beads (50 mg/ml; Sigma Chemical Co.) and incubated at 4°C for 30 min. The nonimmune complex was sedimented by centrifugation and the resultant supernatant incubated with 20 μl of the specific antiserum. After 1 h this mixture was centrifuged in a microfuge (13,000 g, 15 min) and the resultant supernatant mixed with 50 μl of protein A–Sepharose beads (50 mg/ml). After 1 h the immunoprecipitates were washed eight times with extraction buffer by briefly sedimenting in the microfuge, aspirating the supernatant and then resuspending the Sepharose beads again in 0.5 ml of extraction buffer. Finally the Sepharose beads was washed twice in 50 mM Tris-HCl, pH 7.4, before processing for SDS-PAGE.
For limited proteolytic cleavage of myosin VI, A431 cells were labeled as described above with 33P. They were then extracted with a buffer containing 25 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, protease and phosphatase inhibitors (see above), and 1% IGEPAL CA-630 (Sigma Chemical Co.) but no denaturing detergent, because digestion with chymotrypsin in the presence of SDS leads to complete cleavage to small fragments. After immunoprecipitation and washing, pellets were finally washed and resuspended in 25 mM Tris-HCl, pH 7.2, 150 mM NaCl, 1 mM CaCl2, and incubated with Chymotrypsin (Sigma Chemical Co.) at 1:200 (wt/wt) for 30 min at 25°C. The digests were then processed for SDS-PAGE.
Buss F., Kendrick-Jones J., Lionne C., Knight A.E., Côté G.P, & Paul Luzio J. (1998). The Localization of Myosin VI at the Golgi Complex and Leading Edge of Fibroblasts and Its Phosphorylation and Recruitment into Membrane Ruffles of A431 Cells after Growth Factor Stimulation. The Journal of Cell Biology, 143(6), 1535-1545.
Publication 1998
Buffers Cells Centrifugation Chymotrypsin Cultured Cells Cysteine Cytokinesis Deoxycholate Detergents Digestion Edetic Acid HEPES Igepal CA-630 Immune Sera Immunoprecipitation inhibitors Methionine myosin VI Orthophosphate Pellets, Drug Peptide Hydrolases Phosphates Phosphoric Monoester Hydrolases Protease Inhibitors Proteolysis Rabbits Radioactivity SDS-PAGE Sepharose Serum Sodium Chloride Staphylococcal protein A-sepharose Triton X-100 Tromethamine Viscosity
5
Spatial Variation of Water Quality in Shrimp Aquaculture
Cited 4 times
A total of 66 water samples (from 22 enclosed culture ponds with three replicate) were collected from four pacific white shrimp cultural regions in Guangdong and Hainan provinces, China (Figure 1, Table S1). The sampled ponds were of similar size, water depth, and shrimp stocking density. Samples A, B, C, D, E, F, G, T, U, and V were from high salinity cultural areas in Maoming and Dongfang City, and samples H, I, J, K, L, M, N, O, P, Q, R, and S were from low salinity cultural areas in Zhuhai and Guangzhou City. Site locations were recorded via the global positioning system (GPS, Garmin Vista HCx) and the geographical distances between sampling sites ranged from about 50 m to over 683 km. For each sample, 1.0 L of water was taken from a depth of 0.5 m below the surface using sterile bottles, and samples were immediately placed on ice (Hou et al., 2016 (link)) before being filtered through a 0.22 μm polyethersulfone membrane filter (Supor-200, Pall) using a peristaltic pump. The cell pellets on the polyethersulfone membranes were stored at −80°C until DNA extraction. Temperature, pH, DO, and salinity were measured on-site using a YSI handheld multi-parameter instrument (Model YSI 380, YSI Incorporated, USA). For the chemical analyses, 200 mL of each sample was collected from the same location using sterile bottles. The samples were placed on ice, immediately transported to the laboratory, and stored at 4°C. TN, TP concentrations of dissolved inorganic nitrogen ( NH4+ -N, NO2- -N, and NO3- -N), and orthophosphate ( PO43- -P) were measured using an automatic discrete analyzer (Model CleverChem 200, DeChem-Tech, Germany).
Hou D., Huang Z., Zeng S., Liu J., Wei D., Deng X., Weng S., He Z, & He J. (2017). Environmental Factors Shape Water Microbial Community Structure and Function in Shrimp Cultural Enclosure Ecosystems. Frontiers in Microbiology, 8, 2359.
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Publication 2017
DNA Replication Nitrogen Orthophosphate Pellets, Drug Peristalsis polyether sulfone Salinity Sterility, Reproductive Tissue, Membrane

Most recents protocols related to «Orthophosphate»

1
Analyzing Nutrient Composition and Digestibility
The amounts of basal diet (forages) and concentrates offered and refused were individually weighed and recorded on a daily (forages) or weekly (concentrates) basis. The daily feed intake was calculated as the difference between the offered and refused amount for both basal diet and concentrates. Representative weekly samples of forages were collected, pooled per forage type by mixing equal amounts [on a fresh matter (FM) basis], and sent to the certificated laboratory Eurofins Agro NL (Wageningen, the Netherlands) for chemical analysis based on near infrared spectroscopy (NIRS). The concentrates were produced in one batch each, immediately sampled and analyzed. The chemical composition of these forages and concentrate samples was used to calculate the chemical composition of the total rations. During the final week of the experiment and 2 d prior to the start of fecal sampling, additional samples of the forages and concentrates were collected for the determination of the ATTD of chemical constituents. Forages were sampled daily and concentrates every 2 d. Samples were stored at –20 °C until later analysis. At the end of the experiment, forages were thawed at room temperature, pooled per type of forage by mixing equal amounts on a FM basis, freeze-dried for approximately 96 h in a Zirbus sublimator 3-4-5/20 (Zirbus Technology Benelux B. V., Tiel, the Netherlands) and ground to pass through a 1-mm screen using a Retsch ZM200 grinder (Retsch Benelux, Aartselaar, Belgium). The forage and concentrate samples were analyzed by Schothorst Feed Research (Lelystad, the Netherlands). The DM content was determined by drying at 103 °C to constant weight according to method ISO 6496 (ISO, 1998 ). Crude ash was determined gravimetrically after ashing the samples in a muffler furnace for 3 h at 550 °C, according to method ISO 5984 (ISO, 2002 ). The N content was determined by the Dumas method using a macro determinator (LECO CM928 MLC, LECO, Michigan, USA) according to method ISO 16634 (ISO, 2016 ), and the CP content was calculated as N × 6.25. The starch content (except in grass silage) was determined by the amylo-glucosidase method according to the procedures of Englyst et al. (1992) (link), and sugar content was determined according to the Luff-Schoorl method. Crude fat (CFat) was determined by ether extraction after acid hydrolysis, according to method ISO 11085 (ISO, 2015 ). The NDF content was exclusive of residual ash and a heat-stable α-amylase was added during NDF extraction, according to ISO 16472 (ISO, 2006 ). The ADF content was exclusive of ash and determined according to ISO 13906 (ISO, 2008 ). The P content was determined based on the colorimetric method according to ISO 6491 (ISO, 1998 ) and contents of Ca and TiO2 were determined based on atomic absorption spectroscopy according to ISO 6869 (ISO, 2000 ). The content of PP in forages and concentrates was analyzed at Danisco Animal Nutrition Research Centre (Brabrand, Denmark) using the HPLC method described by Christensen et al. (2020) (link) modified from Skoglund et al. (1998) (link). Modifications to the analytical procedure were that the extraction of IP6 from the feces samples was carried out at a concentration of 0.20 g/mL using 1.0M HCl as solvent. The phytase activity in concentrate samples was analyzed by Danisco Animal Nutrition Research Centre (Brabrand, Denmark) according to a modified version of the 2000.12 AOAC method (Engelen et al., 2001 (link)). For this, one FTU was defined as the quantity of enzyme that released 1 µmol of inorganic orthophosphate from a 0.0051 mol/L sodium phytate substrate per minute at pH 5.5 at 37 °C.
Dersjant-Li Y., Kok I., Westreicher-Kristen E., García-González R., Mereu A., Christensen T, & Marchal L. (2023). Effect of a biosynthetic bacterial 6-phytase on the digestibility of phosphorus and phytate in midlactating dairy cows. Journal of Animal Science, 101, skad032.
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Publication 2023
Acids Amylase Animal Nutritional Physiological Phenomena Carbohydrates chemical composition Colorimetry Diet Enzymes Ethyl Ether Feces Feed Intake Freezing Glucosidase High-Performance Liquid Chromatographies Hydrolysis Orthophosphate Phytase Poaceae Silage Sodium Phytate Solvents Spectrophotometry, Atomic Absorption Spectroscopy, Near-Infrared Starch
2
Ibuprofen Capsule Formulation Development
USP grade Ibuprofen, Tween 80 and leucine were purchased from MEDISCA (Plattsburgh, NY, USA) and Sigma Aldrich (Castle Hill NSW, Australia). Lactose monohydrate as InhaLac®120 was purchased from MEGGLE group, Germany. Magnesium stearate was purchased from Professional Compounding Chemists of Australia (PCCA) Pty Ltd. (PCCA, New South Wells, Australia). Hypromellose empty capsule shells (size 3) were purchased as Vcaps® Plus Capsules from LONZA Inc., New Jersey, USA. Absolute ethanol (analytical grade), acetonitrile (HPLC grade) and orthophosphoric acid were purchased form Thermo Fisher Scientific (Queensland, Australia) and, RCI Labscan Limited (South Australia Australia.), Sodium chloride, potassium chloride, potassium di-hydrogen orthophosphate and di-sodium hydrogen phosphate were obtained from MERCK Pty. Limited, Germany for preparing phosphate buffered saline (PBS, pH 7.4).
Sharif S., Muneer S., Izake E.L, & Islam N. (2023). Impact of Leucine and Magnesium Stearate on the Physicochemical Properties and Aerosolization Behavior of Wet Milled Inhalable Ibuprofen Microparticles for Developing Dry Powder Inhaler Formulation. Pharmaceutics, 15(2), 674.
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Publication 2023
acetonitrile Capsule Ethanol High-Performance Liquid Chromatographies Hydrogen Hypromellose Ibuprofen Lactose Leucine magnesium stearate Orthophosphate Phosphates phosphoric acid Potassium Potassium Chloride Saline Solution Sodium Chloride sodium phosphate Tween 80
3
Comprehensive Physicochemical and Heavy Metal Assessment
The physiochemical parameters were determined for each sample. The water temperature (WT), dissolved oxygen (DO), electrical conductivity (EC), oxidation reduction potential (ORP), turbidity (Tur), chlorophyll-a (Chl-a), and pH of the water were measured in situ by using an EXO2 (YSI, Yellow Springs, OH, USA), and the ORP and pH of sediment cores were measured in situ with a portable detector. The total nitrogen (TN), total phosphorus (TP), ammonia nitrogen (NH4+-N), nitrate nitrogen (NO3−-N), nitrite nitrogen (NO2−-N), and orthophosphate (PO43−-P) of water, and TN, TP, NH4+-N, NO3−-N, water content (WC), and organic matter (OM) of sediment were measured by spectrophotometer (for nutrients) and muffle furnace (for OM) based on the standard method [20 ,21 (link)]. The chemical oxygen demand was measured using the potassium permanganate (CODMn) method.
Eleven heavy metals (As, Hg, Fe, Cr, Co, Ni, Cu, Zn, Cd, Pb, and Mn) were measured in the samples of surface water, bottom water, and sediment. Sediment cores were regrouped based on physiochemical parameters (Figure 1), and heavy metals were determined after being regrouped and mixed. The pretreatment and analysis of water and sediment samples were based on a previous study [12 (link)]. Briefly, 45 mL water sample, 4 mL HNO3, and 1 mL HCl were placed in a 100 mL closed Teflon vessel and digested 10 min at 170 °C; 0.1 g sediment sample and 6 mL aqua regia were put into a 100 mL closed Teflon vessel and digested 60 min at 180 °C. After digestion, the heavy metals Hg and As were determined by atomic fluorescence; Zn, Pb, Cu, Mn, Ni, Cd, Cr, Co, and Fe were determined by an inductively coupled plasma emission spectrometer [12 (link)].
Wang C., Wang K., Zhou W., Li Y., Zou G, & Wang Z. (2023). Occurrence, Risk, and Source of Heavy Metals in Lake Water Columns and Sediment Cores in Jianghan Plain, Central China. International Journal of Environmental Research and Public Health, 20(4), 3676.
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Publication 2023
Ammonia aqua regia Blood Vessel Chemical Oxygen Demand Chlorophyll A Digestion Electric Conductivity Fluorescence Metals, Heavy Natural Springs Nitrates Nitrites Nitrogen Nutrients Orthophosphate Oxidation-Reduction Oxygen Phosphorus Plasma Potassium Permanganate Teflon
4
Orthophosphate Quantification in Freeze-Dried Samples
Sub-samples from each freeze-dried nubbin were weighed and digested in a persulfate potassium solution autoclaved at 121 °C [86 (link)]. Samples were then placed in triplicate on a 96-well microplate with a solution of ammonium molybdate and incubated at room temperature for 10 min before the addition of a solution of malachite green. After 30 min, the absorbance was measured at 630 nm using a spectrofluorometer (Xenius®, SAFAS, Monaco) to quantify the orthophosphate content. The standard curve was prepared with known concentrations of potassium phosphate.
Blanckaert A.C., Biscéré T., Grover R, & Ferrier-Pagès C. (2023). Species-Specific Response of Corals to Imbalanced Ratios of Inorganic Nutrients. International Journal of Molecular Sciences, 24(4), 3119.
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Publication 2023
ammonium molybdate Freezing malachite green Orthophosphate potassium persulfate potassium phosphate
5
Water Filtration and Nutrient Analysis
Upon return to the laboratory, all samples were gently rotated ~ 10 × to ensure water was well-mixed prior to processing. Then, using a 30 ml syringe, water from each replicate was filtered through a 25 mm, 0.7 μm pore size glass fiber filter (GF/F) (Whatman®) into acid-washed (as described previously) 20 ml glass scintillation vials (~ 17 ml final volume) for DIN: nitrate + nitrite (NO3− + NO2−; henceforth N + N, ammonia-N (NH3 + NH4+; henceforth AmN), dissolved inorganic phosphorus (DIP): orthophosphate (PO43−), total dissolved N (TDN), and phosphorus (TDP). Sample vials were then frozen (−20 °C) until analysis. Nutrient samples were analyzed within 1 h of thawing using a Hach© Lachat QuikChem 8500 Flow Injection Analysis System (Strickland and Parsons 1984 ; Grasshoff et al. 1999 ). Protocol Minimum Detection Limit (MDLs) were as follows: AmN (0.05 μM; Lachat Applications Group 2017 ), N + N (0.014 μM; Egan 2008 ), PO43− (0.0646 μM; Egan 2007 ), TDN and TDP (0.485 and 0.123 μM, respectively; Tucker et al. 2008a , 2008b ). MDLs were used when measured concentrations were below those values. DON and DOP concentrations were calculated per replicate as TDN–DIN and TDP–DIP, respectively. DOC samples were collected following standard procedures (Ducklow and Dickson 1994 ; JGOFS 1996 ), specifically by filtering water through a 30 ml syringe (previously soaked in 10% HCL for 24 h then rinsed 3 times with ddH2O, equipped with a pre-combusted (2 h at 450 °C) GF/F) into a pre-combusted (4 h at 450 °C) 20 ml glass scintillation vial. Following addition of two to three drops of 10% HCl, vials containing samples were stored (4 °C) prior to analysis (in duplicates), and subsequently analyzed by the University of Wisconsin–Milwaukee. DOM was calculated as the sum of DON, DOP, and DOC.
Humphries G.E., Espinosa J.I., Ambrosone M., Ayala Z.R., Tzortziou M., Goes J.I, & Greenfield D.I. (2023). Transitions in nitrogen and organic matter form and concentration correspond to bacterial population dynamics in a hypoxic urban estuary. Biogeochemistry, 163(2), 219-243.
Publication 2023
Acids Ammonia DNA Replication Flow Injection Analysis Freezing H 450 Nitrates Nitrites Nutrients Orthophosphate Phosphorus Syringes

Top products related to «Orthophosphate»

1
32p orthophosphate by PerkinElmer
Sourced in United States
[32P]orthophosphate is a radioactive isotope of phosphorus that can be used as a tracer in various research and analytical applications. It emits beta particles and is commonly used to label biomolecules, monitor biological processes, and detect specific compounds. The core function of [32P]orthophosphate is to serve as a tool for researchers and analysts to study and quantify the behavior and distribution of phosphorus-containing compounds in biological systems.
2
Disodium hydrogen orthophosphate by Merck Group
Sourced in United States, India
Disodium hydrogen orthophosphate is a chemical compound used as a laboratory reagent. It is a white, crystalline solid that is soluble in water. The compound is a salt of phosphoric acid and is commonly used in various scientific and analytical applications.
3
Simplicity uv system by Merck Group
Sourced in France, United States, Germany, Poland
The Simplicity UV system is a laboratory instrument designed for UV-Visible spectroscopy. It provides accurate and reliable measurements of light absorbance or transmittance across a range of wavelengths in the ultraviolet and visible light spectrum.
4
32p orthophosphate by MP Biomedicals
32P-orthophosphate is a radioactive isotope of phosphorus that is commonly used as a tracer in various laboratory applications. It emits beta particles during its radioactive decay, which can be detected and quantified using specialized equipment. This product is primarily used in research and analytical settings to study biochemical processes, track the movement of phosphorus-containing compounds, and analyze the composition of samples.
5
Potassium dihydrogen orthophosphate by Thermo Fisher Scientific
Sourced in United Kingdom, Australia
Potassium dihydrogen orthophosphate is a chemical compound with the formula KH2PO4. It is a crystalline solid that is commonly used as a buffer solution, pH adjuster, and nutrient in various laboratory and industrial applications.
6
Methanol by Merck Group
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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
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Sodium chloride (nacl) by Merck Group
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NaCl is a chemical compound commonly known as sodium chloride. It is a white, crystalline solid that is widely used in various industries, including pharmaceutical and laboratory settings. NaCl's core function is to serve as a basic, inorganic salt that can be used for a variety of applications in the lab environment.
8
Hydrochloric acid (hcl) by Merck Group
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Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.
9
Hplc grade acetonitrile by Merck Group
Sourced in Germany, United States, India, Italy, United Kingdom, China, Spain, Sao Tome and Principe, Switzerland, Macao, Canada, France, Indonesia, Poland, Argentina, Japan, Australia
HPLC-grade acetonitrile is a high-purity organic solvent commonly used as a mobile phase component in high-performance liquid chromatography (HPLC) applications. It is a colorless, volatile liquid with a characteristic odor. The product meets the specifications required for HPLC-grade solvents, ensuring consistency and reliability in analytical procedures.
10
Ascorbic acid by Merck Group
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Ascorbic acid is a chemical compound commonly known as Vitamin C. It is a water-soluble vitamin that plays a role in various physiological processes. As a laboratory product, ascorbic acid is used as a reducing agent, antioxidant, and pH regulator in various applications.

More about "Orthophosphate"

Orthophosphates, also known as inorganic phosphates or phosphate salts, are a diverse class of chemical compounds that play a vital role in numerous scientific and industrial applications.
These compounds are characterized by the presence of the orthophosphate (PO4^3-) ion, which consists of a central phosphorus atom bonded to four oxygen atoms.
Orthophosphates are widely used in a variety of fields, including biology, chemistry, and environmental science.
In biological systems, they are crucial for energy metabolism, signal transduction, and structural components of cells.
They are also commonly found in detergents, fertilizers, and water treatment applications.
One of the key orthophosphate compounds is [32P]orthophosphate, which is a radioactive isotope of orthophosphate that is commonly used in research and diagnostic applications.
Another important compound is Disodium hydrogen orthophosphate, which is used in a variety of applications, including the Simplicity UV system, a water purification technology.
Potassium dihydrogen orthophosphate is another orthophosphate compound that is used in various applications, such as in the preparation of buffer solutions.
Methanol, NaCl, Hydrochloric acid, and HPLC-grade acetonitrile are also commonly used in conjunction with orthophosphate-related research and applications.
Researchers and professionals working with orthophosphates can leverage the power of PubCompare.ai, an AI-driven platform that helps optimize their research by providing access to protocols from literature, pre-prints, and patents, as well as advanced AI-driven comparisons to identify the most accurate and reproducible protocols and products.
With its intuitive and user-friendly interface, PubCompare.ai can streamline the orthophosphate research process and help researchers and professionals achieve their goals more effectively.