Escherichia coli TOP10 (Invitrogen) and E. coli CA434 [39] (link) were cultured in Luria-Bertani (LB) medium, supplemented with chloramphenicol (25 µg/ml), where appropriate. Routine cultures of C. difficile 630 Δerm[40] (link) and C. difficile R20291 were carried out in BHIS medium (brain heart infusion medium supplemented with 5 mg/ml yeast extract and 0.1% [wt/vol] L-cysteine) [41] (link). C. difficile medium was supplemented with D-cycloserine (250 µg/ml), cefoxitin (8 µg/ml), lincomycin (20 µg/ml), and/or thiamphenicol (15 µg/ml) where appropriate. A defined minimal media [18] (link) was used as uracil-free medium when performing genetic selections. A basic nutritive mannitol broth for growth assays of C. difficile strains were prepared as follows : Proteose peptone no. 2 4% [wt/vol] (BD Diagnostics, USA), sodium phosphate dibasic 0.5%[wt/vol], potassium phosphate monobasic 0.1%[wt/vol], sodium chloride, 0.2% [wt/vol], magnesium sulfate, 0.01% [wt/vol], mannitol, 0.6% [wt/vol] with final pH at +/−7.35. For solid medium, agar was added to a final concentration of 1.0% (wt/vol). Clostridium sporogenes ATCC 15579 was cultivated in TYG media [7] (link). All Clostridium cultures were incubated in an anaerobic workstation at 37°C (Don Whitley, Yorkshire, United Kingdom). Uracil was added at 5 µg/ml, and 5-Fluoroorotic acid (5-FOA) at 2 mg/ml. All reagents, unless noted, were purchased from Sigma-Aldrich.
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Potassium phosphate, dibasic
Potassium phosphate, dibasic
Potassium phosphate, dibasic is an inorganic salt with a wide range of applications in scientific research and industrial processes.
It serves as a buffering agent, pH regulator, and nutrient in cell culture media, biochemical assays, and various other laboratory procedures.
This compound is also used in the production of fertilizers, detergents, and food preservatives.
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One typo: 'literatuer' instead of 'literature'.
It serves as a buffering agent, pH regulator, and nutrient in cell culture media, biochemical assays, and various other laboratory procedures.
This compound is also used in the production of fertilizers, detergents, and food preservatives.
Discover the power of potassium phosphate, dibasic with PubCompare.ai's AI-driven platform, which helps you easily locate the best research protocols from literature, pre-prints, and patents.
Optimize your experiments and find the right products for your needs.
Experience seamless research with PubCompare.ai's intuitive tools and data-driven insights.
One typo: 'literatuer' instead of 'literature'.
Most cited protocols related to «Potassium phosphate, dibasic»
Escherichia coli TOP10 (Invitrogen) and E. coli CA434 [39] (link) were cultured in Luria-Bertani (LB) medium, supplemented with chloramphenicol (25 µg/ml), where appropriate. Routine cultures of C. difficile 630 Δerm[40] (link) and C. difficile R20291 were carried out in BHIS medium (brain heart infusion medium supplemented with 5 mg/ml yeast extract and 0.1% [wt/vol] L-cysteine) [41] (link). C. difficile medium was supplemented with D-cycloserine (250 µg/ml), cefoxitin (8 µg/ml), lincomycin (20 µg/ml), and/or thiamphenicol (15 µg/ml) where appropriate. A defined minimal media [18] (link) was used as uracil-free medium when performing genetic selections. A basic nutritive mannitol broth for growth assays of C. difficile strains were prepared as follows : Proteose peptone no. 2 4% [wt/vol] (BD Diagnostics, USA), sodium phosphate dibasic 0.5%[wt/vol], potassium phosphate monobasic 0.1%[wt/vol], sodium chloride, 0.2% [wt/vol], magnesium sulfate, 0.01% [wt/vol], mannitol, 0.6% [wt/vol] with final pH at +/−7.35. For solid medium, agar was added to a final concentration of 1.0% (wt/vol). Clostridium sporogenes ATCC 15579 was cultivated in TYG media [7] (link). All Clostridium cultures were incubated in an anaerobic workstation at 37°C (Don Whitley, Yorkshire, United Kingdom). Uracil was added at 5 µg/ml, and 5-Fluoroorotic acid (5-FOA) at 2 mg/ml. All reagents, unless noted, were purchased from Sigma-Aldrich.
5-fluoroorotic acid
Agar
Biological Assay
Brain
Cefoxitin
Chloramphenicol
Clostridium
Clostridium sporogenes
Cycloserine
Cysteine
Diagnosis
Escherichia coli
Genetic Selection
Heart
Lincomycin
Mannitol
potassium phosphate
proteose-peptone
Sodium Chloride
sodium phosphate
Strains
Sulfate, Magnesium
Thiamphenicol
Uracil
Yeast, Dried
Acetic Acid
Amino Acids
Buffers
Calcium
Cells
Edetic Acid
Endocytosis
FuGene
HEK293 Cells
Hyperostosis, Diffuse Idiopathic Skeletal
Ions
Magnesium
Microscopy, Confocal
Molar
Nigericin
paraform
Parathyroid Hormone
PHluorin
Potassium Chloride
Protoplasm
Quartz
Radionuclide Imaging
Sodium Chloride
sodium phosphate
Transfection
The chickens were sacrificed under chloroform anesthesia by cervical dislocation. Whole caeca with their contents originating from 18 random healthy chickens or hens 4 to 40 weeks of age were removed during necropsy, chilled on ice and transported to an anaerobic chamber for further processing within one hour. The caeca were opened in an anaerobic chamber (10% CO2, 5% H2 and 85% N2 atmosphere; Concept 400, Baker Ruskinn, USA) and 0.5 g of content was squeezed into 4.5 ml pre-reduced PRAS dilution blank (0.1 g magnesium sulfate heptahydrate, 0.2 g monobasic potassium phosphate, 0.2 g potassium chloride, 1.15 g dibasic sodium phosphate, 3.0 g sodium chloride, 1.0 g sodium thioglycolate, 0.5 g L-cysteine, 1000 ml distilled water; final pH 7.5 +/− 0.2 at 25 °C) and mixed thoroughly. All samples were serially diluted in pre-reduced PRAS dilution blank and plated on Wilkins-Chalgren anaerobe agar (WCHA) (Oxoid) supplemented with 30% of rumen fluid. The rumen fluid was collected from cows by an oral probe, filtered through cheesecloth, centrifuged at 8000 g for 30 min and sterilised by filtration through a 0.22 μm filter. Aliquots of rumen fluid were stored at − 20 °C. WCHA was additionally supplemented with 5 mg/l hemin, 1 mg/l cellobiose, 0.5 g/l soluble starch, 1 mg/ml maltose, 0.2 ml vitamin K1 solution (0.1 ml of filter sterilized vitamin K1 in 20 ml 95% ethanol) and 0.5 mg/ml L-cysteine. Approx. 10 well-separated colonies of different morphology were selected from each agar plate after a five-day incubation at 37 °C and purified by subculture on WCHA. All isolates were stored at − 80 °C in pre-reduced PRAS dilution blank containing glycerol at 20% concentration and equal volume of sterile sheep blood. Sensitivity of pure anaerobe cultures to air oxygen exposure was tested exactly as described elsewhere [6 (link)]. Briefly, bacterial cultures were serially diluted in anaerobic chamber and plated on 4 copies of WCHA. One copy of WCHA was left in the anaerobic chamber to determine initial counts of each anaerobe. The remaining 3 copies of WCHA plates were placed into a standard aerobic 37 °C incubator and after 1, 8 and 24 h, a single copy of agar plate was returned back to the anaerobic chamber to check for growth restoration.
2-N-propylamine-cyclo-hexanethiol
Agar
Anesthesia
Atmosphere
Autopsy
Bacteria
Bacteria, Aerobic
Bacteria, Anaerobic
Blood Volume
Cattle
Cecum
Cellobiose
Chickens
Chloroform
Cysteine
Ethanol
Filtration
Glycerin
Hemin
Heptahydrate Magnesium Sulfate
Hypersensitivity
Joint Dislocations
Maltose
Neck
Oxygen
Potassium Chloride
potassium phosphate, monobasic
Rumen
S100A6 protein, human
Sheep
Sodium Chloride
sodium phosphate, dibasic
sodium thioglycolate
Starch
Sterility, Reproductive
Technique, Dilution
Vitamin K1
Cultures were grown in RM medium (10 g/L yeast extract, 2 g/L KH2PO4) supplemented with either 2% (w/v) glucose (RMG) or 2% (w/v) xylose (RMX) for inhibitor studies. The pH of all media was adjusted to pH5.8 and was filter sterilized. Media were prepared from stock solutions of yeast extract and monobasic potassium phosphate, and when possible, inhibitor stock solutions were prepared and titrated to pH 5.8. Two compounds (4-hydroxycinnamic acid and syringaldehyde) were prepared from stock solutions in 100% DMSO due to their low solubility in water. The total amount of DMSO in the final medium ranged from 0.1-5.0% (v/v) for 4-hydroxycinnamic acid and 0.1%-3% (v/v) in syringaldehyde. Inhibitor studies with DMSO alone did not detect notable inhibitions on growth or final cell mass (data not shown).
Ammonium salts were prepared by titrating the acids with concentrated ammonium hydroxide, except for the anions: sulfate, chloride, nitrate, acetate, phosphate. Calcium formate was prepared by titrating formic acid with lime. Phosphate stocks were made by preparing 1 M stock solutions of monobasic phosphate and titrating the medium to pH of 5.8 with 1 M dibasic solution.
As a consequence of precipitation of monobasic potassium phosphate with calcium, it was not included in medium containing calcium. In this case, 50 mM MES, pH 5.8, was provided to supply some buffering capacity. Growth rates in RMG or RMX with 50 mM MES, pH 5.8, and no potassium phosphate were similar to those obtained in media with potassium phosphate which indicated that yeast extract in rich media could supply sufficient phosphorous for growth at the 2% sugar level (data not shown).
Overnight cultures in RMG medium were either started from single colonies or from glycerol stocks. Optical densities were measured using a Beckman DU-640 spectrophotometer (Beckman Coulter, Inc., Brea, CA) for inoculation. Growth rates were obtained from the Bioscreen C analyzer purchased from Growth Curves USA (Piscataway, NJ). Procedures for inoculation, growth conditions, measurement, recording of final cell densities and calculations used to correct for non-linear response at high cell densities were previously reported [22 (link)]. In brief, log phase cultures of Z. mobilis 8b (a recombinant xylose-utilizing strain of ZM4) were obtained by inoculating overnight cultures in RMG at 30°C and allowing the cells to grow to an OD600 ~ 1.0. Cells were then spun down at 3840 × g, for 10 min at RT and resuspended in RMG or RMX with inhibitor at the desired concentration such that the starting cell density distributed to Bioscreen C microplates after appropriate dilutions with inhibitors was OD600 = 0.05 (~5 × 106 cells/mL=) in a total volume of 300 μL. Incubations were performed at 30°C and absorbance readings were taken every 10 min. Operation of the Bioscreen C and collection of turbidity measurements (OD420–580) were computer automated with EZ Experiment. Data were collected and exported to Microsoft Excel spreadsheets.
Cultures for mini-fermentation studies at 1X MIC were inoculated with Z. mobilis 8b from seed cultures at an OD600 of 1.0 described above, in 4.5 mL of RM medium containing 5% glucose or 5% xylose and inhibitor compounds at a concentration which would cause 100% inhibition of growth rates (1X MIC) in 6 mL HPLC vials at 30°C, 150 rpm, and were vented with an 18 gauge needle and 0.2 micron syringe filter. Samples (0.5 mL) were removed at 0, 24 and 48 hours post inoculation for OD600 and HPLC analysis.
Cultures for aldehyde conversion studies were inoculated with Z. mobilis 8b at an OD600 of 1.0 in 100 mL of RMG containing 5% glucose in 125 mL unbaffled shake flasks containing aldehyde inhibitors at a concentration that would cause 50% inhibition of growth rates at 30°C, 125 rpm. Samples were removed at 0, 24 and 48 hours for HPLC and growth analysis. Flasks containing inhibitor medium without cells were included to assess abiotic loss due to instability or volatility.
Ammonium salts were prepared by titrating the acids with concentrated ammonium hydroxide, except for the anions: sulfate, chloride, nitrate, acetate, phosphate. Calcium formate was prepared by titrating formic acid with lime. Phosphate stocks were made by preparing 1 M stock solutions of monobasic phosphate and titrating the medium to pH of 5.8 with 1 M dibasic solution.
As a consequence of precipitation of monobasic potassium phosphate with calcium, it was not included in medium containing calcium. In this case, 50 mM MES, pH 5.8, was provided to supply some buffering capacity. Growth rates in RMG or RMX with 50 mM MES, pH 5.8, and no potassium phosphate were similar to those obtained in media with potassium phosphate which indicated that yeast extract in rich media could supply sufficient phosphorous for growth at the 2% sugar level (data not shown).
Overnight cultures in RMG medium were either started from single colonies or from glycerol stocks. Optical densities were measured using a Beckman DU-640 spectrophotometer (Beckman Coulter, Inc., Brea, CA) for inoculation. Growth rates were obtained from the Bioscreen C analyzer purchased from Growth Curves USA (Piscataway, NJ). Procedures for inoculation, growth conditions, measurement, recording of final cell densities and calculations used to correct for non-linear response at high cell densities were previously reported [22 (link)]. In brief, log phase cultures of Z. mobilis 8b (a recombinant xylose-utilizing strain of ZM4) were obtained by inoculating overnight cultures in RMG at 30°C and allowing the cells to grow to an OD600 ~ 1.0. Cells were then spun down at 3840 × g, for 10 min at RT and resuspended in RMG or RMX with inhibitor at the desired concentration such that the starting cell density distributed to Bioscreen C microplates after appropriate dilutions with inhibitors was OD600 = 0.05 (~5 × 106 cells/mL=) in a total volume of 300 μL. Incubations were performed at 30°C and absorbance readings were taken every 10 min. Operation of the Bioscreen C and collection of turbidity measurements (OD420–580) were computer automated with EZ Experiment. Data were collected and exported to Microsoft Excel spreadsheets.
Cultures for mini-fermentation studies at 1X MIC were inoculated with Z. mobilis 8b from seed cultures at an OD600 of 1.0 described above, in 4.5 mL of RM medium containing 5% glucose or 5% xylose and inhibitor compounds at a concentration which would cause 100% inhibition of growth rates (1X MIC) in 6 mL HPLC vials at 30°C, 150 rpm, and were vented with an 18 gauge needle and 0.2 micron syringe filter. Samples (0.5 mL) were removed at 0, 24 and 48 hours post inoculation for OD600 and HPLC analysis.
Cultures for aldehyde conversion studies were inoculated with Z. mobilis 8b at an OD600 of 1.0 in 100 mL of RMG containing 5% glucose in 125 mL unbaffled shake flasks containing aldehyde inhibitors at a concentration that would cause 50% inhibition of growth rates at 30°C, 125 rpm. Samples were removed at 0, 24 and 48 hours for HPLC and growth analysis. Flasks containing inhibitor medium without cells were included to assess abiotic loss due to instability or volatility.
Most recents protocols related to «Potassium phosphate, dibasic»
Potassium dihydrogen phosphate (Merck Group), Potassium phosphate dibasic (SPECTRUM), sodium acetate (Merck Group), Tris Base (Fisher Bioreagents), Bis-Tris Hydrochloride (Sigma Aldrich), and sodium chloride (BioLab) were used for buffer preparation.
LC-MS grade water, methanol, acetonitrile, and isopropanol were all purchased from Merck (Darmstadt, Germany). Formic acid, dimethyl sulfoxide (DMSO), potassium phosphate dibasic (K2HPO4), potassium phosphate monobasic (KH2PO4), and bovine serum albumin (BSA) were acquired from Sigma-Aldrich (Buchs, Switzerland).
Sodium alginate (SA) (low viscosity and medium viscosity) was obtained from Sigma Aldrich.
Paracetamol, Carboxymethyl cellulose (CMC) (7M1FPH) was a gift from Ashland, Türkiye. Calcium chloride dehydrates, potassium phosphate dibasic (K HPO ) and potassium phosphate monobasic (KH PO ) were from ISO LAB chemicals and ethanol from LiChrosolv.
Paracetamol, Carboxymethyl cellulose (CMC) (7M1FPH) was a gift from Ashland, Türkiye. Calcium chloride dehydrates, potassium phosphate dibasic (K HPO ) and potassium phosphate monobasic (KH PO ) were from ISO LAB chemicals and ethanol from LiChrosolv.
Recombinant truncated NA protein from the A/Perth/16/2009 H3N2 strain of influenza virus was produced in Chinese hamster ovary (CHO) cell line. The recombinant protein was secreted and purified from clarified supernatants, yielding a homogeneous SEC profile with a major peak corresponding to the molecular size of the tetramer.
Sodium phosphate monobasic and dibasic, potassium phosphate dibasic (all ACS reagents) and monobasic (molecular grade), sodium chloride and sucrose (both BioXtra, ≥ 99.5%), were purchased from Sigma.
Sodium phosphate monobasic and dibasic, potassium phosphate dibasic (all ACS reagents) and monobasic (molecular grade), sodium chloride and sucrose (both BioXtra, ≥ 99.5%), were purchased from Sigma.
Ammonium chloride (NH4Cl), magnesium sulfate heptahydrate (MgSO4·7H2O), calcium chloride dihydrate (CaCl2·2H2O), Sodium phosphate dibasic (Na2HPO4), potassium dihydrogen phosphate (KH2PO4), iron (III) chloride hexahydrate (FeCl3·6H2O), sodium hydroxide (NaOH), sodium bicarbonate (NaHCO3), potassium chloride (KCl), potassium hydroxide (KOH), ethanol, Luria-Bertani medium, agarose gel, osmium acid were purchased from Aladdin Industrial Corporation, China. Anhydrophosphoric acid, 4-aminobenzoic acid, hydrochloric acid, glutaraldehyde, acetone, potassium hexacyanoferrate (III) (K3[Fe(CN)6]), sodium acetate were purchased from Sinopharm Chemical Reagent Co. Ltd. China.
Top products related to «Potassium phosphate, dibasic»
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Potassium phosphate monobasic is a chemical compound used in various laboratory applications. It is a colorless, crystalline solid that is soluble in water. The primary function of potassium phosphate monobasic is to serve as a buffer in pH-sensitive experiments and to maintain specific pH levels in various biological and chemical systems.
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Sodium phosphate dibasic is a chemical compound that is commonly used in laboratory settings. It is a crystalline, white solid that is soluble in water and has a neutral to basic pH. The compound's primary function is to serve as a buffering agent, helping to maintain a specific pH level in various chemical reactions and processes.
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NaCl is a chemical compound commonly known as sodium chloride. It is a white, crystalline solid that is widely used in various industries, including pharmaceutical and laboratory settings. NaCl's core function is to serve as a basic, inorganic salt that can be used for a variety of applications in the lab environment.
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Potassium phosphate dibasic is a chemical compound that is commonly used in various laboratory applications. It is a white crystalline powder that is soluble in water and has a neutral to slightly alkaline pH. The core function of potassium phosphate dibasic is to serve as a buffer agent, helping to maintain a specific pH range in aqueous solutions.
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Potassium chloride (KCl) is an inorganic compound that is commonly used as a laboratory reagent. It is a colorless, crystalline solid with a high melting point. KCl is a popular electrolyte and is used in various laboratory applications.
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Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.
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Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.
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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
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Sodium phosphate monobasic is a chemical compound commonly used as a lab reagent. It is a white, crystalline solid that is soluble in water. The primary function of sodium phosphate monobasic is to act as a pH buffer in various laboratory applications, helping to maintain a specific pH level in solutions.
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.