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Sodium Hypochlorite

Sodium Hypochlorite is a widely-used chemical compound with a broad range of applications, including water treatment, disinfection, and bleaching.
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Most cited protocols related to «Sodium Hypochlorite»

1
Rice Protoplast Isolation Protocol
Cited 64 times
Dehulled seeds of rice (Oryza sativa L.) cultivar Nipponbare were sterilized with 75% ethanol for 1 min. These seeds were further sterilized with 2.5% sodium hypochlorite for 20 min, washed at least five times with sterile water and then incubated on 1/2 MS medium with a photoperiod of 12 h light (about 150 μmol m-2 s-1) and 12 h dark at 26°C for 7-10 days. Green tissues from the stem and sheath of 40-60 rice seedlings were used. A bundle of rice plants (about 30 seedlings) were cut together into approximately 0.5 mm strips with propulsive force using sharp razors. The strips were immediately transferred into 0.6 M mannitol for 10 min in the dark. After discarding the mannitol, the strips were incubated in an enzyme solution (1.5% Cellulase RS, 0.75% Macerozyme R-10, 0.6 M mannitol, 10 mM MES at pH 5.7, 10 mM CaCl2 and 0.1% BSA) for 4-5 h in the dark with gentle shaking (60-80 rpm). After the enzymatic digestion, an equal volume of W5 solution (154 mM NaCl, 125 mM CaCl2, 5 mM KCl and 2 mM MES at pH 5.7) was added, followed by vigorous shaking by hand for 10 sec. Protoplasts were released by filtering through 40 μm nylon meshes into round bottom tubes with 3-5 washes of the strips using W5 solution. The pellets were collected by centrifugation at 1,500 rpm for 3 min with a swinging bucket. After washing once with W5 solution, the pellets were then resuspended in MMG solution (0.4 M mannitol, 15 mM MgCl2 and 4 mM MES at pH 5.7) at a concentration of 2 × 106 cells mL-1, determined by using a hematocytometer. The viability of protoplasts was determined by the FDA staining method as described [44 (link)]. All manipulations above were performed at room temperature.
For isolating protoplasts from etiolated rice seedlings, the sterilized seeds were germinated under light for 3 days, and then moved to the dark for another 4-7 days. The isolation procedure was the same as that for isolation of green tissue protoplasts described above.
Zhang Y., Su J., Duan S., Ao Y., Dai J., Liu J., Wang P., Li Y., Liu B., Feng D., Wang J, & Wang H. (2011). A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes. Plant Methods, 7, 30.
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Publication 2011
Cells Cellulase Centrifugation Digestion Enzymes Ethanol isolation Light Magnesium Chloride Mannitol MS 1-2 Nylons Oryza sativa Pellets, Drug Plant Embryos Protoplasts Seedlings Sodium Chloride Sodium Hypochlorite Staining Stem, Plant Sterility, Reproductive Tissues
2
Ancient DNA Analysis of Bone Samples
Cited 29 times
DNA was extracted from ∼100 mg of bone power after sodium hypochlorite treatment (SI Appendix 2). Sequencing, filtering of the data, and genotyping was carried out as previously described (ref. 3 (link) and SI Appendix 3). Present-day human DNA contamination was estimated using mitochondrial, autosomal data, and from the proportion of DNA fragment mapping on the Y chromosome (SI Appendixes 4 and 5). Demographic histories and split times were estimated using the Pairwise Sequentially Markovian Coalescent (38 (link) and SI Appendix 6) and the F(A|B) method (refs. 35 (link) and SI Appendix 7), respectively, and coestimated with momi2 (39 and SI Appendix 7). HBD tracts were computed as previously published (refs. 35 (link) and SI Appendix 8). Selection analyses were performed using the R packages GOfuncR (https://github.com/sgrote/GOfuncR), ABAEnrichment (ref. 29 (link) and SI Appendix 9), and by computing HKA and a modified PBS statistic in 25-kb windows (SI Appendix 10).
Mafessoni F., Grote S., de Filippo C., Slon V., Kolobova K.A., Viola B., Markin S.V., Chintalapati M., Peyrégne S., Skov L., Skoglund P., Krivoshapkin A.I., Derevianko A.P., Meyer M., Kelso J., Peter B., Prüfer K, & Pääbo S. (2020). A high-coverage Neandertal genome from Chagyrskaya Cave. Proceedings of the National Academy of Sciences of the United States of America, 117(26), 15132-15136.
Publication 2020
Bones DNA Contamination Homo sapiens Mitochondria Sodium Hypochlorite Y Chromosome
3
Transgenic Seed Germination on Sand
Cited 24 times
Flowering plants were grown essentially as described [1 (link)]. Selection of transgenic seeds on agar was on solid MS3 media [4.4 g/L Murashige & Skoog Basal Salt mixture (Sigma-Aldrich cat: M5524), 30 g/L sucrose, 0.5 g/L Monohydrate 2-(N-morpholino)ethanesufonic acid (MES); adjusted pH 5.7, 1.5% phyto agar (Duchefa Biochemie)]. Seed sterilization was as described [1 (link)], except that a 33% commercial bleach/Triton X-100 solution was used [33% DanKlorix (this is ~28 g/L sodium hypochlorite; Colgate-Palmolive; Hamburg) and 200 μL/L Triton X-100].
Selection on sand in a plastic-petri plate started with saturating chromatography sand with liquid MS0 media (1.1 g/L MS basal salt, 0.5 g/L MES; adjusted pH 5.7). The sands reported here were i) Silicon dioxide (SiO2); purum p.a.; acid purified; 40–200 mesh (84880 – Fluka), and ii) Quartz (SiO2); purum p.a.; powder; < 230 mesh (00653 – Sigma). Appropriate antibiotics were added to the MS0 solution before sand saturation. Dry seeds were sprinkled onto the wet sand, and after a two-day treatment at 4°C, plants were allowed to grow for two weeks.
Davis A.M., Hall A., Millar A.J., Darrah C, & Davis S.J. (2009). Protocol: Streamlined sub-protocols for floral-dip transformation and selection of transformants in Arabidopsis thaliana. Plant Methods, 5, 3.
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Publication 2009
Acids Agar Animals, Transgenic Antibiotics, Antitubercular Chromatography Magnoliopsida Morpholinos Plant Embryos Plants Powder Quartz Silicon Dioxide Sodium Chloride Sodium Hypochlorite Sterilization Sucrose Triton X-100
4
Isogenic Drosophila Lines for Wolbachia Research
Cited 21 times

D. melanogaster lines with Wolbachia are described in Table 1. Lines with Wolbachia variants described in Riegler et al.[40] (link) were kindly provided by Markus Riegler and Scott O'Neill. wMelCS_b source and DrosDel w1118 isogenic background were described elsewhere [12] , [59] (link). wMel variants were introduced in the DrosDel w1118 iso isogenic background by chromosomes replacement using a first and third double balancer line and a second chromosome balancer line. The crosses were performed with Wolbachia-infected females, ensuring endosymbiont transmission through the germline. The fourth chromosome was not isogenized. All the Wolbachia genotypes were confirmed by PCR, as described in Riegler et al.[40] (link) (data not shown).
The lines were cleaned of possible chronic viral infections as described elsewhere [12] , [60] .
In order to homogenize the gut microbiota, embryos from each line were sterilized with 2% sodium hypochlorite, followed by 70% ethanol and washed with sterile water. Embryos were placed in new food vials and 150 µl of a bacterial inoculum from a reference stock was added. The inoculum was produced by mixing 5 ml of sterile water with 2 g of food from 10 days old vials containing VF-0058–3 flies [12] , and filtering it to remove eggs and larvae.
Tetracycline-treated lines were cleaned of Wolbachia infection by raising them for two generations in ready-mix dried food (Philip Harris) with 0.05 mg/ml of tetracycline hydrochloride (Sigma). Experiments were performed on lines that were raised without antibiotics for at least 6 generations.
Drosophila lines were maintained on standard cornmeal diet at a constant temperature of 25°C. We focused the analysis on males in the assumption that Wolbachia levels would be more stable in these. Wolbachia is present in ovaries and the sizes of these vary greatly with mating status and physiology of the female.
Chrostek E., Marialva M.S., Esteves S.S., Weinert L.A., Martinez J., Jiggins F.M, & Teixeira L. (2013). Wolbachia Variants Induce Differential Protection to Viruses in Drosophila melanogaster: A Phenotypic and Phylogenomic Analysis. PLoS Genetics, 9(12), e1003896.
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Publication 2013
Antibiotics Bacteria Chromosomes Diet Diptera Drosophila Drosophila melanogaster Eggs Embryo Ethanol Females Food Food, Dried Gastrointestinal Microbiome Genotype Germ Line Infection Larva Males Ovary physiology Sodium Hypochlorite Sterility, Reproductive Tetracycline Tetracycline Hydrochloride Transmission, Communicable Disease Virus Diseases Wolbachia
5
Wheat Seedling Growth and Analysis
Cited 15 times
Wheat (Triticum aestivum L.) seeds were purchased from a certified supplier of local market in the Allahabad district of India. Seeds were surface sterilized in 10% (v/v) sodium hypochlorite solution for 5 min, then washed thoroughly and soaked for 2–4 h in distilled water. After sterilization and soaking, uniform-sized seeds were sown in plastic trays containing sterilized sand. Thereafter, trays were kept in the dark for seed germination at 25 ± 2°C. When germination reached maximum of percentage, seedlings were grown in a growth chamber under photo synthetically active radiation (PAR) of 250 μmol photons m-2 s-1 and 60% relative humidity with 16:8 h light–dark regime at 25 ± 2°C for 8 days. During the growth period, seedlings were sprayed with water whenever required. Uniformed-sized seedlings were used to analyze the impact of NO on various physiological and biochemical parameters under ZnONPs toxicity.
Tripathi D.K., Mishra R.K., Singh S., Singh S., Vishwakarma K., Sharma S., Singh V.P., Singh P.K., Prasad S.M., Dubey N.K., Pandey A.C., Sahi S, & Chauhan D.K. (2017). Nitric Oxide Ameliorates Zinc Oxide Nanoparticles Phytotoxicity in Wheat Seedlings: Implication of the Ascorbate–Glutathione Cycle. Frontiers in Plant Science, 8, 1.
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Publication 2017
Germination Humidity Light physiology Radiation Seedlings Sodium Hypochlorite Triticum aestivum

Most recents protocols related to «Sodium Hypochlorite»

1
Arabidopsis Floral Dip Transformation Protocol
Not available on PMC !

Example 12

Plant transformation—The Arabidopsis thaliana var Columbia (To plants) were transformed according to the Floral Dip procedure [Clough S J, Bent A F. (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16(6): 735-43; and Desfeux C, Clough S J, Bent A F. (2000) Female reproductive tissues were the primary targets of Agrobacterium-mediated transformation by the Arabidopsis floral-dip method. Plant Physiol. 123(3): 895-904] with minor modifications. Briefly, Arabidopsis thaliana Columbia (C010) T0 plants were sown in 250 ml pots filled with wet peat-based growth mix. The pots were covered with aluminum foil and a plastic dome, kept at 4° C. for 3-4 days, then uncovered and incubated in a growth chamber at 18-24° C. under 16/8 hours light/dark cycles. The T0 plants were ready for transformation six days before anthesis.

Single colonies of Agrobacterium carrying the binary vectors harboring the genes of some embodiments of the invention were cultured in YEBS medium (Yeast extract 1 gr/L, Beef extract 5 gr/L, MgSO4*7H2O, Bacto peptone 5 gr/L) supplemented with kanamycin (50 mg/L) and gentamycin (50 mg/L). The cultures were incubated at 28° C. for 48 hours under vigorous shaking to desired optical density at 600 nm of 0.85 to 1.1. Before transformation into plants, 60 μl of Silwet L-77 was added into 300 ml of the Agrobacterium suspension.

Transformation of T0 plants was performed by inverting each plant into an Agrobacterium suspension such that the above ground plant tissue was submerged for 1 minute. Each inoculated T0 plant was immediately placed in a plastic tray, then covered with clear plastic dome to maintain humidity and was kept in the dark at room temperature for 18 hours to facilitate infection and transformation. Transformed (transgenic) plants were then uncovered and transferred to a greenhouse for recovery and maturation. The transgenic T0 plants were grown in the greenhouse for 3-5 weeks until siliques were brown and dry, then seeds were harvested from plants and kept at room temperature until sowing.

For generating T1 and T2 transgenic plants harboring the genes of some embodiments of the invention, seeds collected from transgenic T0 plants were surface-sterilized by exposing to chlorine fumes (6% sodium hypochlorite with 1.3% HCl) for 100 minutes. The surface-sterilized seeds were sown on culture plates containing half-strength Murashig-Skoog (Duchefa); 2% sucrose; 0.5% plant agar; 50 mg/L kanamycin; and 200 mg/L carbenicylin (Duchefa). The culture plates were incubated at 4° C. for 48 hours and then were transferred to a growth room at 25° C. for three weeks. Following incubation, the T1 plants were removed from culture plates and planted in growth mix contained in 250 ml pots. The transgenic plants were allowed to grow in a greenhouse to maturity. Seeds harvested from T1 plants were cultured and grown to maturity as T2 plants under the same conditions as used for culturing and growing the T1 plants.

US11859197B2. Insecticidal polypeptides and use thereof (2024-01-02). EVOGENE LTD. [IL]. Inventors: Or Rotem [IL], Moshe Elbaz [IL], Michal Simovitch Etkes [IL], Lisa N. Meihls [US], Noam Ben Naim [IL], Dhritiman Ghosh [US].
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Patent 2024
Agar Agrobacterium Aluminum Animals, Transgenic Arabidopsis Arabidopsis thalianas Bacto-peptone Beef Chlorine Cloning Vectors Culture Media Decompression Sickness Females Genes Genes, Plant Gentamicin Humidity Infection Kanamycin Marijuana Abuse Plant Diseases Plant Embryos Plants Plants, Transgenic Reproduction Saccharomyces cerevisiae silwet L-77 Sodium Hypochlorite Sucrose Sulfate, Magnesium Tissues
2
Glass Surface Activation Protocol
Not available on PMC !

Example 41

At room temperature, a first glass member and a second glass member were respectively washed in acetone, a peracetic acid solution or a calcium hypochlorite solution in sequence for 30 min, removed, washed with pure water and dried. The washed first glass member and second glass member were treated for 60 min in a mixed solution of sodium hypochlorite and aqueous ammonia (containing, in percentage by weight, 5% of sodium hypochlorite, 5% of aqueous ammonia, and 90% of deionized water) at room temperature. The residual solution on the surface was washed off for 10 min with pure water. Then the glass members were blow dried. In this way, the surfaces of the glass members were activated.

US11858844B2. Glass composite, casing, display device and terminal device (2024-01-02). BYD COMPANY LIMITED [CN]. Inventors: Lan Ma [CN], Liang Chen [CN], Xiong Yuan [CN], Chen Li [CN].
Full text: Click here
Patent 2024
Acetone Ammonia calcium hypochlorite Cocaine Medical Devices Peracetic Acid Sodium Hypochlorite
3
Glass Surface Activation Protocol
Not available on PMC !

Example 39

At room temperature, a first glass member and a second glass member were respectively washed in acetone, a peracetic acid solution or a calcium hypochlorite solution in sequence for 30 min, removed, washed with pure water and dried. The washed first glass member and second glass member were treated for 30 min in a mixed solution of sodium hypochlorite and aqueous ammonia (containing, in percentage by weight, 5% of sodium hypochlorite, 15% of aqueous ammonia, and 80% of deionized water) at room temperature. The residual solution on the surface was washed off for 10 min with pure water. Then the glass members were blow dried. In this way, the surfaces of the glass members were activated.

US11858844B2. Glass composite, casing, display device and terminal device (2024-01-02). BYD COMPANY LIMITED [CN]. Inventors: Lan Ma [CN], Liang Chen [CN], Xiong Yuan [CN], Chen Li [CN].
Full text: Click here
Patent 2024
Acetone Ammonia calcium hypochlorite Cocaine Medical Devices Peracetic Acid Sodium Hypochlorite
4
Hard Water Tolerance of Chlorinated Cleaner
Not available on PMC !

Example 4

Testing to evaluate hard water tolerance of exemplary formulations of a high-foaming, higher alkaline chlorinated cleaner (with and without PSO) was conducted to determine the impact of the PSO on hard water tolerance. The evaluated formulations are shown below in Table 8 wherein alkaline cleaning compositions including hydroxide alkalinity sources were combined with the PSO adducts and compared to the formulations without the PSO adducts (Control).

TABLE 8
EXP 9Control
DI water25-5025-50
NaOH 50%10-3010-30
PSO adducts, 40%1-5 0
Lauryl dimethylamine oxide 30% 5-10 5-10
Sodium Hypochlorite, 10%20-4020-40
Additional Functional Ingredients 5-10 5-10
100.00100

The hardness tolerance testing of the EXP 9 formulation and the control were conducted using 1% solutions in water with varying degrees of synthetic hardness created by adding various amounts of dissolved CaCl2) and MgCl2 to a combination of deionized water and NaHCO3. Once the solutions reached 140° F. they were removed from the heat and let stand for 30 minutes. A failure was characterized by the presence of visible flocculent after the 30 minutes, whereas a passing evaluation was characterized by the absence of visible flocculent after the 30 minutes. The results are shown in Table 9.

TABLE 9
Grains per
Water sourcegallonEXP 9Control
Synthetic hard water16PassPass
Synthetic hard water17PassPass
Synthetic hard water18PassFail
Synthetic hard water19PassFail
Synthetic hard water20Fail—
Synthetic hard water21Fail—
Synthetic hard water22Fail—
Synthetic hard water23Fail—

As shown in Table 10, the exemplary high-foaming formulation (EXP 9) according to the invention containing the PSO adducts had increased hard water tolerance over cleaning compositions not containing the PSO adducts.

The inventions being thus described, it will be obvious that the same may be varied in many ways. Such variations are not to be regarded as a departure from the spirit and scope of the inventions and all such modifications are intended to be included within the scope of the following claims.

US11859155B2. Hard surface cleaning compositions comprising phosphinosuccinic acid adducts and methods of use (2024-01-02). ECOLAB USA INC. [US]. Inventors: Carter M. Silvernail [US], Erik C. Olson [US], Michel M. Lawrence [US], Richard D. Johnson [US], Steven J. Lange [US].
Full text: Click here
Patent 2024
Acids Alkalies Bicarbonate, Sodium Cereals dodecyldimethylamine oxide hydroxide ion Immune Tolerance Magnesium Chloride Sodium Hypochlorite
5
Glass Surface Activation Protocol
Not available on PMC !

Example 40

At room temperature, a first glass member and a second glass member were respectively washed in acetone, a peracetic acid solution or a calcium hypochlorite solution in sequence for 30 min, removed, washed with pure water and dried. The washed first glass member and second glass member were treated for 20 min in a mixed solution of sodium hypochlorite and aqueous ammonia (containing, in percentage by weight, 10% of sodium hypochlorite, 30% of aqueous ammonia, and 60% of deionized water) at room temperature. The residual solution on the surface was washed off for 10 min with pure water. Then the glass members were blow dried. In this way, the surfaces of the glass members were activated.

US11858844B2. Glass composite, casing, display device and terminal device (2024-01-02). BYD COMPANY LIMITED [CN]. Inventors: Lan Ma [CN], Liang Chen [CN], Xiong Yuan [CN], Chen Li [CN].
Full text: Click here
Patent 2024
Acetone Ammonia calcium hypochlorite Cocaine Medical Devices Peracetic Acid Sodium Hypochlorite

Top products related to «Sodium Hypochlorite»

1
Sodium hypochlorite by Merck Group
Sourced in United States, Germany, Canada, France, Sweden, Portugal, Italy
Sodium hypochlorite is a chemical compound that is commonly used as a disinfectant and oxidizing agent in various applications. It has a chemical formula of NaOCl.
2
Sodium hydroxide (naoh) by Merck Group
Sourced in Germany, United States, India, United Kingdom, Italy, China, Spain, France, Australia, Canada, Poland, Switzerland, Singapore, Belgium, Sao Tome and Principe, Ireland, Sweden, Brazil, Israel, Mexico, Macao, Chile, Japan, Hungary, Malaysia, Denmark, Portugal, Indonesia, Netherlands, Czechia, Finland, Austria, Romania, Pakistan, Cameroon, Egypt, Greece, Bulgaria, Norway, Colombia, New Zealand, Lithuania
Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.
3
Sodium hypochlorite solution by Merck Group
Sourced in United States, Germany, Spain
Sodium hypochlorite solution is a chemical compound commonly used as a disinfectant and oxidizing agent. It is a clear, greenish-yellow liquid with a characteristic chlorine-like odor. The solution contains sodium hypochlorite as the active ingredient, which is effective in killing a wide range of bacteria, viruses, and fungi.
4
Osteo assay surface plate by Corning
Sourced in United States
Osteo Assay Surface plates are a type of cell culture labware designed for the study of bone-forming cells. They provide a specialized surface that supports the growth and differentiation of osteoblasts, the cells responsible for bone formation. These plates are a tool used in various research applications related to bone biology and development.
5
Hydrochloric acid (hcl) by Merck Group
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Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.
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Phytagel by Merck Group
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Phytagel is a natural polysaccharide derived from Sphingomonas paucimobilis bacteria. It is a versatile gelling agent used in various laboratory applications, including cell and tissue culture, microbiology, and biochemistry. Phytagel forms clear, stable gels that can withstand a wide range of pH, temperature, and ionic conditions.
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Ethanol by Merck Group
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Ethanol is a clear, colorless liquid chemical compound commonly used in laboratory settings. It is a key component in various scientific applications, serving as a solvent, disinfectant, and fuel source. Ethanol has a molecular formula of C2H6O and a range of industrial and research uses.
8
Sodium bromide by Merck Group
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Sodium bromide is an inorganic compound with the chemical formula NaBr. It is a white crystalline solid that is highly soluble in water. Sodium bromide is commonly used as a laboratory reagent and in the production of other chemical compounds.
9
Whatman filter paper by Cytiva
Sourced in United Kingdom, United States, Japan, Germany, Switzerland, China, India
Whatman filter paper is a laboratory filtration product designed for various filtering applications. It is manufactured to provide consistent quality and performance. The core function of Whatman filter paper is to separate solid particles from liquids or gases through the process of filtration.
10
Tween 20 by Merck Group
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Tween 20 is a non-ionic detergent commonly used in biochemical applications. It is a polyoxyethylene sorbitan monolaurate, a surfactant that can be used to solubilize and stabilize proteins and other biomolecules. Tween 20 is widely used in various laboratory techniques, such as Western blotting, ELISA, and immunoprecipitation, to prevent non-specific binding and improve the efficiency of these assays.

More about "Sodium Hypochlorite"

Sodium hypochlorite, commonly known as bleach, is a widely-used chemical compound with a broad range of applications in water treatment, disinfection, and bleaching.
This versatile substance is a powerful oxidizing agent that can effectively kill a wide variety of microorganisms, including bacteria, viruses, and fungi.
In addition to its use in water purification and as a disinfectant, sodium hypochlorite is also employed in the production of other chemicals, such as sodium hydroxide (caustic soda) and sodium bromide.
It can be found in a variety of household and industrial products, including cleaning solutions, laundry detergents, and pool chemicals.
The optimization of sodium hypochlorite research can be greatly enhanced by utilizing the AI-driven comparison platform offered by PubCompare.ai.
This powerful tool allows researchers to effortlessly locate and compare protocols from literature, pre-prints, and patents, enabling the identification of the most effective products and processes for their specific needs.
By leveraging the intelligent comparisons provided by PubCompare.ai, researchers can streamline their sodium hypochlorite optimization efforts, saving time and resources while ensuring the use of the most effective and efficient methods.
This one-stop-shop for sodium hypochlorite optimization can be a game-changer for scientists and industry professionals working with this versatile and important chemical compound.