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Sodium sulfite

Sodium sulfite is an inorganic compound with the chemical formula Na2SO3.
It is a white, crystalline powder that is commonly used as a food preservative, antioxidant, and reducing agent.
Sodium sulfite is known to have a variety of applications in food, pharmaceutical, and industrial settings.
It is an important compound in research, with studies exploring its effects on various biological and chemical processes.
Researchers can use PubCompare.ai's AI-driven protocol optimization to discover the most reproducible and effective sodium sulfite research protocols from published literature, pre-prints, and patents, streamlining their research and enhancing reproducibilty.
Experice the power of data-driven decision making today!

Most cited protocols related to «Sodium sulfite»

Nutrient Digestibility Assessment in Horses

Cited 11 times

Nutrient digestibility was assessed through the use of internal (acid detergent insoluble ash, ADIA) and external (Titanium IV Dioxide, rutile; Sigma–Aldrich) markers. On d 27, baseline fecal samples were collected. From d 28 to 41, 5 g of titanium dioxide (TiO₂) was mixed into each concentrate meal. After a 10-d supplementation period, feces were collected from d 38 to 41 of the study. To account for diurnal variation of marker concentration, feces were collected twice daily at 12-h intervals, advancing by 3 h each subsequent day, which resulted in 8 fecal samples per mare over a 4-d collection period. After each collection time point, a 200 to 400 g sample of feces was stored at −20 °C before analysis.
To prepare samples for analysis, feces, hay, and grain were dried in a forced air oven at 55 °C for 96 h and allowed to equilibrate to room temperature for 24 h. All samples were weighed before and after the drying process to determine partial dry matter (DM) percentage. Samples were ground in a Wiley mill through a 1 mm screen. To determine overall DM percentage, samples were exposed to a 105 °C drying oven for 24 h. Similarly, to determine organic matter (OM) percentage, difference in sample weight was measured before and after exposure to a combustion oven at 450 °C for 8 h. The NDF and ADF values were determined by the Ankom Fiber Analyzer with sodium sulfite and α-amylase admitted and without correction for residual ash (Ankom Technology Corp., Macedon, NY). A Parr 6300 Calorimeter (Parr Instrument Company, Moline, IL) was used to measure gross heat energy (GE) for hay, grain, and fecal samples.
Forage consumption was estimated by TiO₂ analysis following a previously established protocol (Myers et al., 2004 (link)). Concentrate, hay, and fecal samples were ashed at 450 °C for 12 h then exposed to concentrated sulfuric acid (95% to 98% w/w) for 2 h before the addition of 30% hydrogen peroxide solution. Samples were digested at 350 °C for 45 min in a SCP digester. Absorbance at 410 nm was measured and compared with standards of 10, 8, 6, 4, or 2 mg TiO₂ per 50 g solution. The following calculations were used to estimate voluntary DMI of nutrients in the diet: mg TiO₂ / g = (mg TiO_{2 sample} / g sample) − (mg TiO_{2 baseline} / g sample); fecal output (kg/d) = (10 g/d TiO₂) / ([TiO₂] feces (g/kg), where [TiO₂] indicates concentration of TiO₂ in the feces; and DMI (kg/d) = fecal output (kg/d) × ([ADIA] feces/[ADIA] feed), where [ADIA] indicates concentration of ADIA.

Much M.L., Leatherwood J.L., Zoller J.L., Bradbery A.N., Martinez R.E., Keegan A.D., Lamprecht E.D, & Wickersham T.A. (2019). Influence of diet fortification on body composition and apparent digestion in mature horses consuming a low-quality forage. Translational Animal Science, 4(1), 1-9.

Publication 2019

Acids Amylase Cereals Circadian Rhythms Detergents Diet Feces Fibrosis Nutrients Peroxide, Hydrogen rutile sodium sulfite Suby's G solution Sulfuric Acids titanium dioxide

Cellulase Production and Enzymatic Characterization

Cited 7 times

Cellulase was produced in a 500 mL flask that contained 100 mL of fluid medium through a two-step cultivation procedure. Strains were first grown at 30°C in 100 mL of medium that contained 2 g of glucose as a carbon source and were then regulated at pH 5.5 and 200 rpm for 20 hours. The cultures were collected through vacuum drum filtration during this second step, and 0.5 g vegetative mycelia was added to 100 mL of Vogel’s medium that contained 2% cellulose as carbon source or wheat bran medium at an initial pH of 5.5 at 30°C and 200 rpm. Culture supernatants (crude enzyme) were diluted with sodium acetate buffer solution (SABF, 0.2 M, pH 4.8). Enzymatic hydrolyses of the polysaccharides were also performed in SABF (0.2 M, pH 4.8). The filter paper enzyme (FPA), endoglucanase (CMCase), xylanase, and amylase activities of the culture supernatants (diluted samples) were assayed using a DNS reagent (10 g 3, 5-dinitrosalicylic acid, 20 g sodium hydroxide, 200 g sodium potassium tartrate, 2.0 g redistilled phenol, and 0.50 g sodium sulfite anhydrous per 1000 mL DNS reagent) against Whatman No. 1 filter paper, carboxymethylcellulose sodium salt (CMC-Na), xylan (from beechwood), and soluble starch. CMC-Na, xylan, or starch was dissolved in SABF to a final concentration of 1% (mass/volume percent, m/v %), and then the mixture was left overnight and was shaken well before using. The following components were added in a 2.0 mL reaction mixture: 0.5 mL diluted culture supernatants and 1.5 mL CMC-Na, xylan, or starch solution for CMCase, xylanase, or amylase activity assays, respectively; and 2.0 mL diluted culture supernatants and 50 mg Whatman No. 1 filter paper for FPA assay into 25 mL colorimetric tube. The mixture was mixed gently and the reaction mixture was incubated for FPA measurement in a 50°C water bath for 1 hour, for CMCase and xylanase activity measurements at 50°C for 30 min, and for amylase activity measurement at 40°C for 10 min. Three milliliters of DNS reagent were then added to stop the reaction. A blank tube (with boiled crude enzyme) was used as control to correct any reducing sugar present in the crude enzyme samples. The tubes were placed in boiling water for 10 min, 20 mL distilled water was added, 200 μL of reaction mixture was pipetted, and the absorbance was determined at 540 nm. The cellobiohydrolase (pNPCase) and β-glucosidase (pNPGase) activities were measured by using 4-Nitrophenyl β-D-cellobioside (pNPC) and 4-Nitrophenyl β-D-glucopyranoside (pNPG) as substrates, respectively. The pNPC or pNPG was dissolved in SABF to a final concentration of 1 mg/mL. Moreover, 50 μL of pNPC solution (containing 1 mg/mL D-Glucono-δ-lactone) or 50 μL of pNPG solution and 100 μL of diluted culture supernatants were mixed, and then the mixtures were incubated in a 50°C water bath for 30 min. The reaction was stopped by adding 0.15 mL of sodium carbonate solution (10%, m/v), then 200 μL of these reaction mixtures was pipetted, and the absorbance was measured at 420 nm. One unit of enzyme activity was defined as the amount of enzyme required to release 1 μmol of glycoside bonds of the substrate per minute under defined assay conditions. Independent triplicate cultures were sampled and analyzed.
The total protein was determined using a Bradford assay kit according to the instructions of the manufacturer.

Li Z., Yao G., Wu R., Gao L., Kan Q., Liu M., Yang P., Liu G., Qin Y., Song X., Zhong Y., Fang X, & Qu Y. (2015). Synergistic and Dose-Controlled Regulation of Cellulase Gene Expression in Penicillium oxalicum. PLoS Genetics, 11(9), e1005509.

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Publication 2015

4-nitrophenyl 4-nitrophenyl beta-cellobioside 4-nitrophenylgalactoside Acids Amylase Bath beta-Glucosidase Biological Assay Buffers Carbohydrates Carbon carboxymethylcellulase Cardiac Glycosides Cellulase Cellulose Colorimetry enzyme activity Enzymes Exo-Cellobiohydrolase Filtration Glucose Hydrolysis Lactones Mycelium Phenol Polysaccharides Proteins Sodium Acetate sodium carbonate Sodium Carboxymethylcellulose Sodium Chloride Sodium Hydroxide sodium potassium tartrate sodium sulfite Starch Strains Vacuum Wheat Bran Xylanase C Xylans

Microfluidic Device for Oxygen Monitoring

Cited 7 times

Details of device fabrication and cell culture can be found in the Electronic Supporting Information (S1 and S2). The Visisens handheld microscope was mounted vertically in a variable incubator and the microfluidic devices (bonded to sensor foils and filled with collagen gel and cells) were placed on top. The oxygen partial pressure in the device was monitored in the central 5 by 2.5 mm² area including the gel and adjoining media channels. All oxygen measurements were evaluated using the Visisens software (AnalytiCal 1, VA 1.11 prototype version) after calibration against two reference solutions. Oxygen-free solution was prepared by dissolving 1 g of sodium sulfite in 100 ml water with 50 ul of Co(NO₃)₂ standard solution (1g l⁻¹ in 0.5 M nitric acid). Water bubbled with air for 30 min was used as a second calibration point (air-saturated). Due to the opaqueness of the oxygen-sensitive coating, control devices with a glass coverslip bottom were fabricated to facilitate visualization of cell morphology and distribution (Fig. S1). Excellent viability over 2 days of culture in all devices was observed, confirming earlier results with human endothelial cells.^{25 (link)} Phase-contrast images were taken with a CCD color digital camera (Olympus DP72, Japan) connected to an inverted Olympus IX71 microscope. Images were captured with DP2 BSW Software (version 2.2) and analyzed by ImageJ software (Scion Corp., USA).
The oxygen consumption in the devices during EC or HEP culture was simulated using a commercial finite element software (COMSOL Multiphysics v4.2). Transport of diluted species equation (Fick’s law) was adopted as the governing equation, considering the molar concentration of oxygen c as dependent variable. The computational mesh consisted of ~1.8×10⁶ tetrahedral elements with a total volume of 410 mm³. To reduce the calculation time, only a quarter of the device was considered by imposing a symmetry condition on the two cutting planes. The cells were represented as a thin (10 μm) layer on top of the PDMS glue layer. A no-flux condition was implemented to represent the impermeable oxygen sensor foil. A constant concentration of oxygen (17%) was considered as boundary condition on the surfaces exposed to the external environment (fluid channel and gel injection inlet). A time dependent simulation was solved for a period of 4 h at 10 min intervals. The diffusion coefficients and initial oxygen concentration in the different materials are summarized below.

Ochs C.J., Kasuya J., Pavesi A, & Kamm R.D. (2014). Oxygen Levels in Thermoplastic Microfluidic Devices during Cell Culture. Lab on a chip, 14(3), 459-462.

Publication 2014

Cell Culture Techniques Cells Collagen Diffusion Endothelial Cells Fingers Homo sapiens Light Microscopy Medical Devices Microchip Analytical Devices Microscopy Microscopy, Phase-Contrast Molar Nitric acid Oxygen Oxygen-17 Oxygen Consumption Partial Pressure sodium sulfite

Characterization of Diverse Lignin Samples

Cited 6 times

The following chemicals were obtained from Sigma–Aldrich Handels GmbH, Darmstadt, Germany: LS lignosulfonic acid sodium salt (average M_w≈54 000, M_n≈6000 g mol⁻¹), lithium bromide (≥99 %), and dimethyl sulfoxide (HPLC grade). PSS sodium salt and pullulan standards were obtained from Polymer Standard Service (PSS, Mainz, Germany). Technical lignin samples were kindly provided by associated companies: two softwood kraft lignins—Indulin AT (MeadWestvaco Corp., USA), Lignoboost (Innventia/RISE, Sweden), and soda lignin—Sarkanda (Granit S.A., Switzerland); two Organosolv lignins—OSL HW, hardwood (Fraunhofer CBP, Germany), and OSL Alcell, mixed hardwood (Repap, Canada); two lignosulfonates from different sulfite processes—Ammonium LS (Borregaard, Norway) and Magnesium spruce LS (Lenzing, Austria). Biolignin is based on wheat straw Organosolv processing (CIMV, France). Purified pine milled wood lignin (MWLp) was extracted according to the original procedure53 and purified according to the protocol described by Balakshin et al.54 DHP and trimer as side products of coniferyl alcohol polymerization were obtained according to the literature.55 A hexameric lignin model compound was obtained according to Kilpeläinen et al.56

Zinovyev G., Sulaeva I., Podzimek S., Rössner D., Kilpeläinen I., Sumerskii I., Rosenau T, & Potthast A. (2018). Getting Closer to Absolute Molar Masses of Technical Lignins. Chemsuschem, 11(18), 3259-3268.

Publication 2018

Acids ammonium sulfite coniferyl alcohol High-Performance Liquid Chromatographies indulin AT Kraft lignin Lignin lignosulfonates lithium bromide Magnesium Picea Pinus Polymerization Polymers pullulan Sodium Sodium Chloride Sulfoxide, Dimethyl Triticum aestivum

Dietary Emulsifiers Affect Mouse Metabolism

Cited 6 times

Mice were exposed to CMC, P80 or sodium sulfite diluted in the drinking water (1.0% or indicated concentration) (not blinded). The same water (reverse-osmosis treated Atlanta city water) was used for the water-treated (control) group. These solutions were changed every week. Emulsifier solutions were autoclaved for experiments presented in figure 4A-H. When required, emulsifier solutions were replaced by water after 8 weeks of treatment for an additional 6 weeks. Body weights were measured every week and are expressed as % compare to the initial body weight (day 0) defined as 100%. Mice developing colitis (Lcn2 level ≥ 500 ng/g of feces) were excluded for metabolic syndrome parameters analysis. Fresh feces were collected every week for downstream analysis. After 3 months of emulsifier treatment, mice were fasted for 5-h at which time blood was collected by retrobulbar intraorbital capillary plexus. Hemolysis-free serum was generated by centrifugation of blood using serum separator tubes (Becton Dickinson, Franklin Lakes, NJ). Mice were then euthanized, and colon length, colon weight, spleen weight and adipose weight were measure. Organs were collected for downstream analysis. Incorporation of CMC and P80 in the diet was performed by Research Diets Inc. (New Brunswick, NJ, USA) using Purina Rodent Chow diet #5001. These diets are now referenced by Research Diets as C13050701 and C13050702, respectively.

Chassaing B., Koren O., Goodrich J., Poole A., Srinivasan S., Ley R.E, & Gewirtz A.T. (2015). Dietary emulsifiers impact the mouse gut microbiota promoting colitis and metabolic syndrome. Nature, 519(7541), 92-96.

Publication 2015

Aftercare BLOOD Capillaries Centrifugation Colitis Colon Diet Feces Hemolysis Metabolic Syndrome X Mus Obesity Osmosis Rodent Serum sodium sulfite Spleen

Most recents protocols related to «Sodium sulfite»

Bromothymol Blue Redox Reaction

Not available on PMC !

Didodecyldimethylammonium bromide (DDAB, >98.0%) was purchased from Tokyo Chemical Industry Co., Ltd. Tokyo, Japan, and the other chemicals were purchased from Fujifilm Wako Chemical Corporation. Osaka, Japan. Sodium hydroxide (>97%), sodium bromate (>99.5%), sodium hydrogen sulfite (guaranteed reagent), sodium sulfite (guaranteed reagent), manganese(II) sulfate pentahydrate (>99.9%), and bromothymol blue (BTB) (guaranteed reagent) were used. The solutions of the phosphate buffer standard solution (pH = 6) and 0.04-w/v% bromothymol blue solution (BTB) were purchased from Fujifilm Wako Chemical Corporation. Osaka, Japan. All the chemicals were used without further purification.

Shimada M., Someya R., Okamoto Y., Yamamoto D., & Shioi A. (2024). The Reversible Transformation of a Vesicular Aggregate in Response to a pH Oscillation. Processes, 12(3), 514-514.

Publication 2024

Solvent Impact on Extraction Efficiency

Not available on PMC !

Testing the influence of some solvents (usually used in the tanning process) on the extraction efficiency: water, aqueous solution of sodium hydroxide (0.5% w/v), and mixed aqueous solution of sodium sulfite (0.25% w/v) and sodium bisulfite (0.25% w/v);

Quaratesi I., Călinescu I., Lavric V., Ferrara V., Badea E., Chipurici P., Dumbravă E., Constantinescu R., Ignat N.D., & Asofiei I. (2024). Loop-Ultrasound-Assisted Extraction: An Efficient Approach for the Recovery of Bioactive Compounds from Oak Bark. Agronomy, 14(7), 1452-1452.

Publication 2024

Biotin Switch Assay for Protein S-Nitrosation

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Publication 2024

Sulfite-Assisted Bamboo Pretreatment

GL-sulfite pretreatment was carried out in a 200 mL polytetrafluoroethylene (PTFE) reactor after steam pretreatment. Briefly, 10 g of dried SE treated bamboo was mixed directly with 150 mL of water containing different GL loadings (3–12 mL per g-DS (dry substrate)) and 6 g of sodium sulfite was added to the mixtures. Then the reactor was loaded in a large stainless steel tank and fixed well. The system including reactor and stainless steel tank was placed in a chamber equipped with a shaft. The whole system was heated to desired temperature (e.g. 80 °C, 100 °C and 120 °C) with an average rate of 5 °C min⁻¹. Moreover, one control pretreatment without sodium sulfite (with only addition of 7.5 mL per g-DS GL) were also performed at 100 °C. After 1 h, the insoluble residues were collected by filtration and washed with distilled water until neutral pH. Some of the washed samples were dried in an oven at 105 °C for 6 h to evaluate the yield.
The composition percentage of pretreated bamboos based on the initial bamboos can be obtained by multiplying the yield by the percentage of each component of GL pretreated bamboos.

Xing Y., Jin Y., Li H., Jiang J, & Shao B. (2024). Enhancing enzymatic digestibility of bamboo residues using a combined low severity steam explosion and green liquor-sulfite pretreatment. RSC Advances, 14(11), 7609-7615.

Publication 2024

Preparation of Buffered Extract and Dilution for Food Analysis

Sodium sulfite (Na₂SO₃) and sodium dodecyl sulfate (SDS) were supplied by Kanto Chemical Co., Inc. (Tokyo, Japan). Tris(hydroxymethyl)aminomethane hydrochloride (Tris), hydrochloric acid (HCl), polyxyethylene-sorbitan monolaurate (Tween 20), Ethylenediaminetetraacetic acid trisodium salt (EDTA-3Na), 2-mercaptoethanol (2-ME), sodium chloride (NaCl), polyvinylpyrrolidone 25 (PVP25), Sucrose and acetone were supplied by Nacalai Tesque, Inc. (Kyoto, Japan). Bovine serum albumin (BSA) was supplied by FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan), xylitol and methyl alpha-d-glucopyranoside were supplied by Sigma-Aldrich Japan (Tokyo, Japan), horseradish peroxidase (HRP) was supplied by Toyobo Co., Ltd. (Osaka, Japan), Proclin^TM 300 (Proclin), 3,3′,5,5′-tetramethylbenzidine (TMB) was supplied by Surmodics, Inc. (Eden Prairie, MN, USA), 4-Hydroxy phenoxy was supplied by Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan) and LIPIDURE®-BL405 was supplied by NOF Corporation (Tokyo, Japan) respectively. Food samples were extracted, and the extracts were diluted, using the extraction buffer solution (Tris-based neutral buffer solution containing 0.1 M sodium sulfite and 0.6 % (v/v) SDS, 0.1 % (w/v) BSA, 0.04 % (w/v) Tween, 200 mM EDTA-3Na and 0.05 % Proclin) and sample diluent (Tris-based neutral buffer solution, 0.1 % (w/v) BSA, 0.04 % (w/v) Tween, 200 mM EDTA-3Na and 0.05 % Proclin). The following buffers were also prepared: 20 mM Tris-HCl (pH 7.4) containing 0.1 M sodium sulfite and 0.6 % (v/v) SDS (buffer A), and 20 mM Tris-HCl (pH 7.4), 150 mM NaCl, 0.05 % Tween 20, 0.1 % 4-Hydroxy phenoxy acetic acid, 0.05 % (v/v) Proclin, 0.125 % (w/v) LIPIDURE®-BL405 containing 1 % (w/v) BSA (buffer B).

Tomiki M., Sakai M., Tanaka D., Hosoya M., Uchida K., Shibata H., Morita M., Ito R., Iwasaki Y, & Akiyama H. (2024). A reliable enzyme-linked immunosorbent assay for the determining of sesame proteins in raw food ingredients and in processed foods. Food Chemistry: X, 21, 101231.

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Publication 2024

Top products related to «Sodium sulfite»

Sodium sulfite by Merck Group

Sourced in United States, Germany, United Kingdom, Canada

Sodium sulfite is a chemical compound used as a laboratory reagent. It is a white, crystalline solid that is soluble in water. Sodium sulfite serves as a reducing agent and an antioxidant, and it is commonly used in various scientific and industrial applications.

Sodium hydroxide (naoh) by Merck Group

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Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.

Ankom 200 fiber analyzer by ANKOM Technology

Sourced in United States

The Ankom 200 Fiber Analyzer is a lab equipment designed to analyze the fiber content of various samples. It provides quantitative measurements of neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL) in a rapid and automated manner.

Hydrochloric acid (hcl) by Merck Group

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Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.

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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.

Sodium hydroxide (naoh) by Sinopharm

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Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, crystalline solid that is highly soluble in water. Sodium hydroxide has a wide range of applications in various industries, including as a pH regulator, cleaning agent, and chemical intermediate.

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Gallic acid is a naturally occurring organic compound that can be used as a laboratory reagent. It is a white to light tan crystalline solid with the chemical formula C6H2(OH)3COOH. Gallic acid is commonly used in various analytical and research applications.

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Sulfuric acid is a highly corrosive, colorless, and dense liquid chemical compound. It is widely used in various industrial processes and laboratory settings due to its strong oxidizing properties and ability to act as a dehydrating agent.

Dimethyl sulfoxide (dmso) by Merck Group

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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.

Ethanol by Merck Group

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Ethanol is a clear, colorless liquid chemical compound commonly used in laboratory settings. It is a key component in various scientific applications, serving as a solvent, disinfectant, and fuel source. Ethanol has a molecular formula of C2H6O and a range of industrial and research uses.

More about "Sodium sulfite"

Sodium sulfite (Na2SO3) is a widely used inorganic compound with a variety of applications in food, pharmaceutical, and industrial settings.
As a white, crystalline powder, it serves as a food preservative, antioxidant, and reducing agent.
Researchers can leverage PubCompare.ai's AI-driven protocol optimization to discover the most reproducible and effective sodium sulfite research protocols from published literature, preprints, and patents, streamlining their work and enhancing reproducibility.
Sodium sulfite is closely related to other compounds like sodium hydroxide (NaOH), which is also used in research and industry.
The Ankom 200 Fiber Analyzer, for example, utilizes sodium sulfite and other chemicals like hydrochloric acid (HCl), methanol (CH3OH), gallic acid (C6H2(OH)3COOH), and sulfuric acid (H2SO4) to analyze the fiber content of various samples.
In addition to its preservative and antioxidant properties, sodium sulfite has been studied for its effects on biological and chemical processes.
Researchers may use dimethyl sulfoxide (DMSO) or ethanol (C2H5OH) as solvents when investigating the impact of sodium sulfite on different systems.
By leveraging PubCompare.ai's advanced data-driven approach, scientists can identify the most reproducible and effective sodium sulfite research protocols, streamlining their work and enhancing the overall quality and reliability of their findings.
Experice the power of data-driven decision making today!