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Superparamagnetic Iron Oxide Nanoparticles

Superparamagnetic Iron Oxide Nanoparticles (SPIONs) are a class of nano-scale materials that exhibit unique magnetic properties, making them highly versatile for a wide range of biomedical applications.
These nanoparticles are composed of iron oxide and demonstrate superparamagnetic behavior, meaning they can be magnetized in the presence of an external magnetic field but retain no residual magnetism once the field is removed.
This property allows for precise control and targeted delivery of SPIONs within the body.
PubCompare.ai's AI-driven platform empowers researchers to optimize their SPION-based protocols by locating the most effective products and enhancing reproducibility.
Discover the power of data-driven research and take your SPION studies to new heights today!

Most cited protocols related to «Superparamagnetic Iron Oxide Nanoparticles»

1
Nanoparticle Characterization and Validation
Cited 6 times
ISDD was verified against experimentally measured particle transport data for three different particles (polystyrene, iron oxide, silica) from three independent studies utilizing particles of different density, size and agglomeration state. Each study used a different method for quantifying particle transport (see Kinetic Data). This approach limits the potential for method-dependent bias. Superparamagnetic iron oxide nanoparticles with a manufacturer reported diameter of 30 nm (20 nm core by transmission electron microscopy (TEM)), with ~10 nm polymer coating) were obtained from Ocean Nanotechnologies (Springdale, AS). Particle size was verified by Dynamic Light Scattering (DLS) in MilliQ water and RPMI media. DLS sizing was conducted using a custom built high-sensitivity DLS instrument, enabling size-class determination at low particle concentrations (10 μg/mL) similar to those used in in vitro experiments. The instrument is a modification of the instrument developed by our team [32 (link)], and its accuracy was verified against polystyrene beads (Polysciences Inc, Warrington PA, Cat# 16905). The original instrument was enhanced by introducing optic fibers and avalanche photodiodes to improve the collecting efficiency.
Carboxylated fluorescent polystyrene spheres with manufacturer reported diameters of 24, 100, 210, 500 and 1100 nm in diameter were obtained from Invitrogen/Molecular Probes. Particles were virtually monodisperse in our experimental system (fluorescence microscopy, see Particokinetic Data) and could be described according to their reported primary particle size.
The amorphous silica nanoparticles used by Lison et al. [33 (link)] to generate the cellular dose data simulated here had a reported particle size of 29.3 nm (TEM) and a hydrodynamic diameter of 34.8 nm in the study media, DMEM.
Hinderliter P.M., Minard K.R., Orr G., Chrisler W.B., Thrall B.D., Pounds J.G, & Teeguarden J.G. (2010). ISDD: A computational model of particle sedimentation, diffusion and target cell dosimetry for in vitro toxicity studies. Particle and Fibre Toxicology, 7, 36.
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Publication 2010
Avalanches Cells ferric oxide Hydrodynamics Hypersensitivity Kinetics Microscopy, Fluorescence Molecular Probes Polymers Polystyrenes Silicon Dioxide Superparamagnetic Iron Oxide Nanoparticles Transmission Electron Microscopy
2
Fabrication of Lipid-Magnetic Nanovesicles
Cited 5 times
25 mg of 1-stearoyl-rac-glycerol (Sigma-Aldrich), 2.5 mg of oleic acid (Sigma-Aldrich), 2.5 mg of 1,2-dipalmitoyl-rac-glycero-3-phosphocholine (Sigma-Aldrich), 4 mg of mPEG-DSPE5k (Sigma-Aldrich), and 2.5 mg of temozolomide (Sigma-Aldrich) (when TMZ-LMNVs are fabricated), are mixed with 84.5 μl of an ethanol solution of superparamagnetic iron oxide nanoparticles (15 wt%; US Research Nanomaterials Inc.), inside a 6 ml glass vial. Subsequently, the vial is placed inside an ultrasonic bath (Elmasonic S 35w) set at 70 °C in order to melt the lipids and to allow ethanol to evaporate. After ethanol evaporates, 3 ml of a pre-warmed (70 °C) Tween® 80 (Sigma-Aldrich) solution (1.0 wt%) were added to the lipid mixture and immediately sonicated using an ultrasonic homogenizer (Fisherbrand™ Q125 Sonicator) for 15 min (amplitude 30%, 120 W). After the ultrasonic homogenization, the hot mixture is transferred to a high pressure homogenizer (HPH, EmulsiFlex-B15 from Avestin), where the sample is further homogenized by passing it 5 times through the homogenizer at a pressure of 100 000 psi. After the homogenization, the LMNVs are placed for 30 min at 4 °C to allow the lipid-based structures to stabilize. The LMNVs are purified by centrifugation and washing with ultrapure (Mili-Q) water (3 times for 30 min at 4 °C).
Tapeinos C., Marino A., Battaglini M., Migliorin S., Brescia R., Scarpellini A., De Julián Fernández C., Prato M., Drago F, & Ciofani G. (2018). Stimuli-responsive lipid-based magnetic nanovectors increase apoptosis in glioblastoma cells through synergic intracellular hyperthermia and chemotherapy †Electronic supplementary information (ESI) available. See DOI: 10.1039/c8nr05520c. Nanoscale, 11(1), 72-88.
Publication 2018
ACTR protein, human Bath Centrifugation Ethanol Glycerin Lipids monomethoxypolyethylene glycol Oleic Acid Phosphorylcholine Pressure Superparamagnetic Iron Oxide Nanoparticles Tween 80 Ultrasonics
3
Synthesis and Functionalization of Dextran-Coated SPIONs
Cited 4 times
Dextran-coated SPIONs (SPIONDEX) were synthesized in a cold gelation process, as described previously.26 In brief, an aqueous solution containing 8.8% (w/w) dextran, 2.4% (w/w) FeCl3·6H2O, and 0.9% (w/w) FeCl2·4H2O was prepared and filtered through a 0.22-µm membrane into an ice-cooled three-neck round-bottomed flask. Under an argon atmosphere, the temperature was adjusted to 0°C–4°C. NH3 was added to the solution, which resulted in a green-brownish suspension. After heating the solution at 75°C, the formed particles were cooled and dialyzed against water (MWCO 10 kDa) to remove excess salts. Redundant dextran was removed afterward by ultrafiltration in a centrifuge 5430R (Eppendorf, Hamburg, Germany) with Vivaspin20 filter units at 6,300× g for 10 minutes for multiple runs. The dextran molecules of the SPION coating were crosslinked with ECH. In this reaction, the pH of the particles was adjusted to basic conditions with 5 M NaOH and then ECH was added to a final concentration of 15% (v/v). The suspension was stirred for 24 hours and then it was again dialyzed against water for 24 hours, followed by ultrafiltration and sterile filtration through 0.22 µm filters.
The dextran coating of the particles was functionalized with carboxylic acid groups according to Huynh et al.23 Briefly, the pH of the suspension was adjusted to 12–13 with 5 M NaOH. After cooling the suspension, monochloroacetic acid was added and then it was heated to 60°C for 90 minutes, followed by neutralization with acetic acid. In the end, the particles were purified by dialysis against water and ultrafiltration. In the following, they are referred to as SPIONCMD.
The determination of the particles’ iron content was performed photometrically as described by Dokuzovic.27
Unterweger H., Subatzus D., Tietze R., Janko C., Poettler M., Stiegelschmitt A., Schuster M., Maake C., Boccaccini A.R, & Alexiou C. (2015). Hypericin-bearing magnetic iron oxide nanoparticles for selective drug delivery in photodynamic therapy. International Journal of Nanomedicine, 10, 6985-6996.
Publication 2015
Acetic Acid Argon Atmosphere Carboxylic Acids chloroacetic acid Cold Temperature Dextran Dialysis Filtration Iron Neck Salts Sterility, Reproductive Superparamagnetic Iron Oxide Nanoparticles Tissue, Membrane Ultrafiltration
4
Synthesis of Magnetic Iron Oxide Nanoparticles and Their Surface Functionalization
Cited 4 times
SPIONs were synthesized by a modified Massart method, based on chemical coprecipitation of Fe2+ and Fe3+ ions in an alkaline solution.19 In this procedure, 2.15 g of FeCl2·4H2O and 5.8 g of FeCl3 were dissolved separately in 200 mL of deionized water. The two solutions were then mixed and heated to 75°C; 7.5 mL of ammonium hydroxide (25%) was then added to the solution, followed by further heating for 30 minutes. Fe3O4@Au were obtained by a modification of the K-gold method,20 whereby 0.6 g of uncoated nanoparticles were dispersed in 20 mL of deionized water, with subsequent addition of 45 mmol NaOH, 7.85 mmol HAuCl4, 6.6 mmol NaBH4, and 20 mmol citric acid. Next, the nanoparticles were washed three times with water and ethanol. Aminosilane-functionalized nanoparticles (Fe3O4@NH2) were synthesized using a one-step polycondensation method. One gram of uncoated nanoparticles were redispersed in ethanol under argon gas protection. The solution of nanoparticles was then mixed with 2 mL of ammonium hydroxide and 0.5 mL of 3-aminopropyl trimethoxysilane in 2 L of ethanol under a flow of argon gas (5.0 Air Liquide, Houston, TX, USA). After 5 hours, the prepared Fe3O4@NH2 were washed three times with ethanol. Following synthesis, all the nanoparticle samples were placed in an oven at 60°C and dried into powder over 12 hours.
Niemirowicz K., Swiecicka I., Wilczewska A.Z., Misztalewska I., Kalska-Szostko B., Bienias K., Bucki R, & Car H. (2014). Gold-functionalized magnetic nanoparticles restrict growth of Pseudomonas aeruginosa. International Journal of Nanomedicine, 9, 2217-2224.
Publication 2014
Ammonium Hydroxide Anabolism Argon Citric Acid Ethanol Gold gold tetrachloride, acid Ions Oxide, Ferrosoferric Powder Superparamagnetic Iron Oxide Nanoparticles trimethoxysilane
5
Electroporation-based Exosome Cargo Loading
Cited 4 times

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Publication 2013
Electroporation Therapy Exosomes Iron Medical Devices Proteins Pulse Rate Sucrose Superparamagnetic Iron Oxide Nanoparticles Trehalose

Most recents protocols related to «Superparamagnetic Iron Oxide Nanoparticles»

1
Morphological Characterization of SPIONs
Transmission Electron Microscopy (TEM, Jeol-Jem-1011 microscope), working at an accelerating voltage of 100 kV and equipped with a Quemesa Olympus CCD 11 Mp Camera, was utilized for the morphological characterization of as-synthesized SPIONs and SPION@PDA NPs. Organic-capped SPIONs, dispersed in chloroform, were deposited on 300-mesh carbon-coated Cu grid by dipping, while 5 µL of SPION@PDA NPs dispersed in ultrapure water was deposited by casting. Statistical analysis using freeware Image J analysis program was performed to evaluate average size of the “as synthesized” SPIONs. Staining of SPION@PDA NP samples was achieved by using an aqueous phosphotungstic acid solution 2% (w/v).
Siciliano G., Turco A., Monteduro A.G., Fanizza E., Quarta A., Comparelli R., Primiceri E., Curri M.L., Depalo N, & Maruccio G. (2023). Synthesis and Characterization of SPIONs Encapsulating Polydopamine Nanoparticles and Their Test for Aqueous Cu2+ Ion Removal. Materials, 16(4), 1697.
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Publication 2023
Carbon Chloroform Microscopy MP 11 Phosphotungstic Acid Superparamagnetic Iron Oxide Nanoparticles Transmission Electron Microscopy
2
FTIR Analysis of SPIONs and Derivatives
Changes in composition of various NPs were recorded on a Nicolet 6700 infrared detector (Thermo Fisher Scientific, USA). SPIONs, SPION@Cs, SPION@Cs-PEG-FA and SPION@Cs-PEG-FA were pressed with KBr to obtain the pellets at a pressure of 300 kg/cm2. The FTIR spectra of the above sample were obtained by averaging 32 interferograms within the range of 1000–4000 cm–1 with a resolution of 2 cm–1.
Al-Obaidy R., Haider A.J., Al-Musawi S, & Arsad N. (2023). Targeted delivery of paclitaxel drug using polymer-coated magnetic nanoparticles for fibrosarcoma therapy: in vitro and in vivo studies. Scientific Reports, 13, 3180.
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Publication 2023
Pellets, Drug Pressure Spectroscopy, Fourier Transform Infrared Superparamagnetic Iron Oxide Nanoparticles
3
Structural Analysis of SPIONs and SPION@Cs-PEG-FA
The crystal structures of SPIONs and SPION@Cs-PEG-FA were studied by XRD on a RIGAKU X-ray diffractometer (model Miniflex IL, USA) applying the CuKα as a radiation source. All samples were measured at a voltage of 30 kV, current of 25 mA, scan range of 5°–65°, and scan interval of 1°/min.
Al-Obaidy R., Haider A.J., Al-Musawi S, & Arsad N. (2023). Targeted delivery of paclitaxel drug using polymer-coated magnetic nanoparticles for fibrosarcoma therapy: in vitro and in vivo studies. Scientific Reports, 13, 3180.
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Publication 2023
Radiation Radiography Radionuclide Imaging Superparamagnetic Iron Oxide Nanoparticles
4
Catalytic Activity of SPIONs and mSPIONs

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Publication 2023
Bath Fluorescence Fluorescent Probes Free Radicals Hydroxy Acids Hydroxyl Radical Microscopy, Fluorescence Peroxide, Hydrogen Superparamagnetic Iron Oxide Nanoparticles terephthalic acid
5
Transwell-Based BBB Model for Nanoparticle Uptake

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Publication 2023
Cell Culture Techniques Culture Media Decompression Sickness Resistance, Electrical Superparamagnetic Iron Oxide Nanoparticles

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SPIONs (Superparamagnetic Iron Oxide Nanoparticles) are a class of nanomaterials composed of iron oxide cores. They exhibit superparamagnetic properties, allowing them to be magnetized in the presence of an external magnetic field and to return to a non-magnetized state when the field is removed. SPIONs can be used in various laboratory applications due to their unique physical and chemical characteristics.
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Sodium hydroxide (naoh) by Merck Group
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More about "Superparamagnetic Iron Oxide Nanoparticles"

Superparamagnetic Iron Oxide Nanoparticles (SPIONs) are a class of nano-scale materials with unique magnetic properties that make them highly versatile for a wide range of biomedical applications.
These nanoparticles, composed of iron oxide, exhibit superparamagnetic behavior, allowing them to be magnetized in the presence of an external magnetic field while retaining no residual magnetism once the field is removed.
This property enables precise control and targeted delivery of SPIONs within the body.
Researchers can optimize their SPION-based protocols by utilizing PubCompare.ai's AI-driven platform, which empowers them to locate the most effective products and enhance reproducibility.
This data-driven approach can take SPION studies to new heights, unlocking the full potential of these remarkable nanomaterials.
Beyond SPIONs, researchers can also leverage other advanced tools and techniques to further enhance their SPION-based research.
The Zetasizer Nano ZS, for example, can be used to measure the size and zeta potential of SPION samples, providing valuable insights into their physicochemical properties.
The H-600 transmission electron microscope can be employed to visualize the morphology and structure of SPIONs at the nanoscale, while the JEM-2100 can be used for high-resolution imaging.
The synthesis and functionalization of SPIONs often involve the use of chemicals like sodium hydroxide and hydrochloric acid, as well as polymers like poly-L-lysine, which can be used to modify the surface properties of the nanoparticles.
Analytical techniques, such as the Agilent 4200 MP-AES, can be utilized to quantify the elemental composition of SPION samples, while LS columns can be employed for the purification and separation of these nanoparticles.
By combining the powerful capabilities of PubCompare.ai's AI-driven platform with a diverse range of advanced tools and techniques, researchers can unlock new possibilities in the field of SPION-based biomedical research, driving innovation and enhancing the impact of their work.