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2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose

2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose is a synthetic glucose derivative that contains a nitrobenzoxadiazole moiety.
It is used as a fluorescent label for tracking glucose uptake and metabolism in biological systems.
The nitrobenzoxadiazole group provides a chromophore that can be detected using fluorescence microscopy or spectroscopy.
This compound is a valuable tool for stydying glucose homeostasis and metabolic pathways in health and disease.

Most cited protocols related to «2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose»

Comprehensive Analysis of EBV Infection

Cited 6 times

Detailed descriptions of the materials and methods are provided in SI Appendix and include the following: eukaryotic cell lines; virus supernatants from the HEK293 2089 cell line; collection and quantitation of virus supernatant from the B95-8 cell line; preparation of B cells from adenoid tissue; purification of B cells on MACS columns; primary B cells infection with EBV; TMRE and Annexin V detection; glucose uptake using 2-NBDG; FACS sorting for naïve B cells; analysis of the cells’ diameter; cell cycle analysis; calculation of the cell DI; Western blot protein quantification with the Wes platform technology; FACS staining for surface B cell markers; preparation of cell lysates; sample collection for time-course RNA-seq experiments; RNA isolation; library preparation and sequencing; and bioinformatic analysis, including transcript quantification by Salmon, quality control and data normalization, identification of DE genes, gene clustering, GO analysis, heatmaps of log₁₀-transformed expression data, PCA of phenotypic data, PCA with viral genes, clustering of viral genes, and data availability.

Mrozek-Gorska P., Buschle A., Pich D., Schwarzmayr T., Fechtner R., Scialdone A, & Hammerschmidt W. (2019). Epstein–Barr virus reprograms human B lymphocytes immediately in the prelatent phase of infection. Proceedings of the National Academy of Sciences of the United States of America, 116(32), 16046-16055.

Publication 2019

2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose Adenoids Annexin A5 B-Lymphocytes cDNA Library Cell Cycle Cell Lines Cells Epstein-Barr Virus Infections Eukaryotic Cells Genes Genes, Viral Glucose HEK293 Cells isolation Phenotype Proteins RNA-Seq Salmon Specimen Collection Tissues Virus Western Blot

Measuring Cellular Glucose Uptake

Cited 5 times

Cells seeded in 6-well plates were treated with or without 1 μM gefitinib or erlotinib. Cell were washed with PBS three times and then incubated with glucose-free medium for 4 h. After wash with PBS for three times, the cells were incubated with PBS containing 2-NBDG (100 μM; Sigma-Aldrich) for 20 min at 37 °C and then subjected to flow cytometry analysis (BD Biosciences) or microscope image processing (ThermoFisher).

Cheng F.J., Chen C.H., Tsai W.C., Wang B.W., Yu M.C., Hsia T.C., Wei Y.L., Hsiao Y.C., Hu D.W., Ho C.Y., Li T.S., Wu C.Y., Chou W.Y., Yu Y.L., Tang C.H., Chen C.Y., Chen C.M., Hsu J.L., Chen H.F., Chen Y., Tu C.Y., Hung M.C, & Huang W.C. (2020). Cigarette smoke-induced LKB1/AMPK pathway deficiency reduces EGFR TKI sensitivity in NSCLC. Oncogene, 40(6), 1162-1175.

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Publication 2020

2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose Cells Erlotinib Flow Cytometry Gefitinib Glucose Microscopy

In Vivo 2-NBDG Glucose Uptake Imaging

Cited 5 times

After 6 hours of breathing room air or hypoxic gas, mice were anesthetized using isofluorane mixed with 21% oxygen (1.5% v/v) administered through a nose cone. Once breathing was stable, mice were placed on a heating pad for the duration of the experiment. Trans-illumination images, RFP fluorescence (for 4T1-RFP cells) and a background image at 525 nm corresponding to pre-NBDG injection were recorded. Any background fluorescence at this wavelength was due to FAD. 100 µl of 2-NBDG (2.5 mg/ml) dissolved in sterile saline was injected through the tail vein. The 2-NBDG fluorescence was recorded for 75 minutes as follows: continuously for the first 8 minutes, every 30 seconds for the next 30 minutes and every 3 minutes for the final 35 minutes of imaging.
High resolution images of 2-NBDG uptake and constitutive tumor RFP fluorescence were recorded using a Zeiss upright confocal microscope. 2-NBDG fluorescence was excited at 488 nm and imaged from 510–550 nm. RFP fluorescence was recorded from 580–620 nm. All images were collected using a 10×objective (NA = 0.45). Field of view for the first set of images (baseline –40 minutes) was 850×850 µm. After 40 minutes, field of view was 297×297 µm.

Rajaram N., Frees A.E., Fontanella A.N., Zhong J., Hansen K., Dewhirst M.W, & Ramanujam N. (2013). Delivery Rate Affects Uptake of a Fluorescent Glucose Analog in Murine Metastatic Breast Cancer. PLoS ONE, 8(10), e76524.

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Publication 2013

2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose 6-deoxy-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminoglucose Cells Fluorescence Hypoxia Light Microscopy, Confocal Mus Neoplasm Metastasis Neoplasms Nose Oxygen-21 Retinal Cone Saline Solution Sterility, Reproductive Tail Veins

Quantifying Glucose Uptake in INS-1 Cells

Cited 4 times

A fluorescent glucose analogue, 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-glucose (2-NBDG) (Invitrogen #N13195), was used to measure glucose uptake in INS-1 cells according to the manufactory's instructions. Briefly, a 100 µM of 2-NBDG (dissolved in ethanol) was added over the transfected cells (as described above) (100 µM/1 ml medium/well) and incubated for one hour at 37 °C. Next, trypsin harvested cells were washed twice with cold-PBS, then centrifuged for 5 min at 1500 rpm at 4 °C. The supernatant was aspirated and cell suspended in 200 µl of cold-PBS in each tube. Cells were immediately analyzed and quantified by flow cytometry (BD FACS Aria™ III) at excitation 465 nm and emission at 540 nm .

El-Huneidi W., Anjum S., Mohammed A.K., Unnikannan H., Saeed R., Bajbouj K., Abu-Gharbieh E, & Taneera J. (2021). Copine 3 “CPNE3” is a novel regulator for insulin secretion and glucose uptake in pancreatic β-cells. Scientific Reports, 11, 20692.

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Publication 2021

2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose 2-Deoxyglucose 6-deoxy-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminoglucose Cells Cold Temperature Ethanol Flow Cytometry Glucose NRG1 protein, human Trypsin

Glucose Uptake Assay in 3T3-L1 Adipocytes

Cited 3 times

The 3T3L1 fibroblast cell monolayers were differentiated in culture to adipocytes as reported previously [28 (link)]. 50 µL of 200 µg/mL reconstituted plant extract with or without 100 nM insulin was added to each well of 3T3-L1 differentiated cells and incubated for 30 min at 37 °C in an atmosphere of 5% CO₂ and 95% air. 2-NBDG, 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (50 nM) was added. After 5 min, the wells were washed with ice-cold PBS and three to four coverslips were mounted in each slide and blocked with nail polish. Four images were taken of the four corners of the coverslips using a 10× magnification on the microscope. The fluorescence intensity was measured.

Ansari P., Flatt P.R., Harriott P, & Abdel-Wahab Y.H. (2020). Evaluation of the Antidiabetic and Insulin Releasing Effects of A. squamosa, Including Isolation and Characterization of Active Phytochemicals. Plants, 9(10), 1348.

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Publication 2020

2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose 2-Deoxyglucose 3T3-L1 Cells Adipocytes Atmosphere Cold Temperature Fibroblasts Fluorescence Insulin Microscopy Nails Plant Extracts

Most recents protocols related to «2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose»

Murine Myeloid Cell Isolation Protocol

Estradiol benzoate (HY-B1192) was purchased from MCE. Gox (G7141), collagenase type IV (C5138) and DNase I (11284932001) were purchased from Sigma-Aldrich. Fluorochrome-labeled or unlabeled monoclonal antibodies against mouse Ly6G (108454, 127607), IgG2b isotype control (400675), CD16/32 (101319), CD45 (103113), CD11b (101227), F4/80 (123115), FcγR III (158013) were purchased from BioLegend. Recombinant anti-myeloperoxidase antibody (ab208670) was purchased from Abcam. DMEM/F-12 GlutaMAX (SH30023.01) was purchased from Hyclone. 2-NBDG (N13195) was purchased from Invitrogen. Cell Counting Kit-8 (E-CK-A362) was purchased from Elabscience. BSA-FITC, BSA-ICG were purchased from Xi’an ruixi Biological Technology Co., Ltd.

Zhu S., Zhang J., Xue N., Zhu X., Li F., Dai Q., Qing X., Chen D., Liu X., Wei Z, & Cao Y. (2023). Highly specific neutrophil-mediated delivery of albumin nanoparticles to ectopic lesion for endometriosis therapy. Journal of Nanobiotechnology, 21, 81.

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Publication 2023

2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose Antibodies, Anti-Idiotypic Biopharmaceuticals Collagenase Deoxyribonuclease I estradiol 3-benzoate Fluorescein-5-isothiocyanate Fluorescent Dyes IgG2B Immunoglobulin Isotypes ITGAM protein, human Monoclonal Antibodies Mus Peroxidase

Evaluating Glucose Uptake in L6 Cells

We used a cell-based fluorescently-labeled deoxyglucose analog kit (2-NBDG kit, Cayman Chemical, USA) to evaluate whether compounds 1–11 increased glucose uptake in L6 cells.^49,50 Before the experiment, the differentiated L6 myotube was inoculated into a 96-well plate at a density of 1 × 10⁴–4 × 10⁴ cells/well. After 2 h of starvation in serum-free α-MEM medium, we add 100 μL MEM-α medium, which contained different drugs, to each well, and cells were incubated for 12 h to 100% fusion. After overnight incubation, cells were treated with insulin (100 nM), compounds 1–11 (30 μg mL⁻¹) or normal control (0.1% DMSO) in 100 μL glucose-free α-MEM containing 150 μg mL⁻¹ 2-NBDG. At the end of the treatment, plates were centrifuged for five minutes at 400 g at room temperature. The supernatant was aspirated, and 200 μL of cell-based assay buffer was added to each well. Plates were centrifuged for five minutes at 400 g at room temperature. Then the supernatant was aspirated, and 100 μL of cell-based assay buffer was added to each well. The 2-NBDG taken up by cells was detected with fluorescent filters (excitation/emission = 485/535 nm).

He J., Tang P., Liu M., Liao G., Lu R, & Yang X. (2023). Triterpenoid saponins and C21 steroidal glycosides from Gymnema tingens and their glucose uptake activities. RSC Advances, 13(11), 7503-7513.

Publication 2023

2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose 2-Deoxyglucose 6-deoxy-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminoglucose Biological Assay Buffers Caimans Cells Glucose Insulin Pharmaceutical Preparations Serum Skeletal Myocytes Sulfoxide, Dimethyl

Transwell Permeability Assay Protocol

Assays were performed in DPBS-H (10 mM HEPES, 25 mM glucose, in Dulbecco's phosphate-buffered saline with calcium chloride and magnesium chloride) in 24-well transwell plates on a rocking shaker at 20 rpm, 37 °C, 95% humidity, and 5% CO₂. All substrates were dissolved at specific concentrations in DPBS-H and added to the luminal (A) or abluminal (B) side. The incubation times for the substrate were 15, 30, 45, and 60 min. The concentrations of the substrates were measured by a fluorescence microplate reader (Fluoroskan Ascent FL, Thermo Fisher Scientific). Digoxin, dantrolene, and salazosulfapyridine samples were pretreated with acetonitrile precipitation of proteins and measured using an LC-MS/MS system (ExionLC-QTRAP6500+, SCIEX, Framingham, Massachusetts, USA). The compound concentrations were as follows: rhodamine 123 (10 μM), Hoechst 33,342 (200 μM), 2-NBDG (100 μg/ml), digoxin (5 μM), dantrolene (5 μM), and salazosulfapyridine (sulfasalazine, SASP) (5 μM).
In this study, we employed P_e as the permeability coefficient because researchers can eliminate the influence of the insert membranes. The permeability coefficient (P_e) was calculated according to Nakagawa et al. [16 (link)] by dividing the amount of substrate in the luminal compartment (A) by the substrate concentration in the abluminal compartment (B). The volume was obtained at multiple timepoints according to the following formula: where [C]r is the amount of the compound in the receiving compartment, [C]d represents the amount of the compound in the donor compartment, and [R] is the volume of the receiving compartment. When the volume is plotted over time, the slope equals the permeability × surface area product (PS) of the membrane. The PS of the membrane with cells is called the total PS (PS_total), and the PS of the membrane without cells is called the membrane PS (PS_mem). The P_e can be computed from the PS_total and PS_mem:
1/PS_e = 1/PS_total-1/PS_mem where the units of PS and surface area are μL/mL and cm², respectively.
To calculate P_e (cm/min), the PS_e value was divided by the surface area (S) of the membrane:

Nakayama-Kitamura K., Shigemoto-Mogami Y., Toyoda H., Mihara I., Moriguchi H., Naraoka H., Furihata T., Ishida S, & Sato K. (2023). Usefulness of a humanized tricellular static transwell blood–brain barrier model as a microphysiological system for drug development applications. - A case study based on the benchmark evaluations of blood-brain barrier microphysiological system. Regenerative Therapy, 22, 192-202.

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Publication 2023

2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose acetonitrile Biological Assay Calcium chloride calcium phosphate Calcium Phosphates Cells Chlorides Dantrolene Digoxin Fluorescence Glucose HEPES Humidity Magnesium Chloride Permeability Phenobarbital Phosphates Proteins Rhodamine 123 Saline Solution Sulfasalazine Tandem Mass Spectrometry Tissue, Membrane Tissue Donors TXN protein, human

Comprehensive In Vitro Blood-Brain Barrier Model

HBMEC/ci18, HBVPC/ci37, and HASTR/ci35 were established and supplied by Prof. Furihata. VascuLife complete medium was purchased from Kurabo (Osaka, Japan). Astrocyte growth medium, Neurobasal medium, fibronectin, anti-TfR antibody (#13–6800), and rhodamine 123 were purchased from Thermo Fisher Scientific (Waltham, USA). Pericyte medium was purchased from ScienCell Research Laboratories (Carlsbad, CA, USA). Blasticidin S was purchased from Fujifilm Wako (Tokyo, Japan). Collagen IV and collagen I were purchased from Nitta Gelatin (Osaka, Japan). Anti-Claudin-5 (ab131259), anti-P-gp (ab170904), and anti-Glut1 (ab115730) antibodies were purchased from Abcam (Cambridge, UK). Anti-β-actin antibody was purchased from Sigma–Aldrich (A5316, St. Louis, MO, USA). Anti-CD31 antibody was purchased from Proteintech (66065-1-Ig, Rosemont, IL, USA). Anti-ZO-1 antibody was purchased from Invitrogen (#339100). Anti-BCRP antibody was purchased from Cell Signaling Technology (#4477, Danvers, MA, USA). Anti-rabbit IgG conjugated with Alexa Fluor 488 or 594, anti-goat IgG conjugated with Alexa Fluor 488, and anti-mouse IgG conjugated with Alexa Fluor 488 or 594 were purchased from Molecular Probes. Fetal bovine serum (FBS) and Dulbecco's modified Eagle's medium (DMEM) were purchased from Life Technologies (Grand Island, NY, USA). Can Get Signal was purchased from TOYOBO (Osaka, Japan). Hoechst 33,342 and DAPI were purchased from Dojindo (Tokyo, Japan). 2-NBDG was purchased from Cayman Chemical Company (Ann Arbor, Michigan, USA). Digoxin was purchased from Alfer Aeser (Heysham, Lancashire, UK). Dantrolene and salazosulfapyridine (sulfasalazine, SASP) were purchased from Tocris Bioscience (Minneapolis, MN, USA). Adenosine 3′,5′-cyclic monophosphate sodium salt monohydrate was purchased from Merck (Darmstadt, Germany). Both human transferrin with no conjugated fluorophore and human transferrin conjugated with Alexa Fluor 488 were purchased from Jackson ImmunoResearch (West Grove, USA).

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Publication 2023

2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose Actins Adenosine alexa fluor 488 anti-IgG Antibodies Antibodies, Anti-Idiotypic Astrocytes blasticidin S Caimans Claudin-5 Collagen Type I Collagen Type IV Dantrolene DAPI Digoxin Fetal Bovine Serum Fibronectins Gelatins Goat Homo sapiens Molecular Probes Mus Pericytes Rabbits Rhodamine 123 SLC2A1 protein, human Sodium Sodium Chloride Sulfasalazine Transferrin TXN protein, human

2-NBDG Glucose Uptake Assay

The 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) cell-based glucose uptake assay was performed. Briefly, following pre-treatment with BADGE (20 μM) for 1 hour, the cells were treated with 100 μg/mL of CLA/CLA_GS4 in glucose free DMEM for 48 hours. Thirty minutes before the end of the treatment, 2-NBDG was added to a final concentration of 100 μg/mL in glucose free medium. The cells were harvested, and assay was performed as according to the manufacture's guidelines (Cayman Chemical, Michigan, USA). The fluorescence was recorded using fluorospectrometric with fluorescent filter (excitation/emission = 485 nm/535 nm). The results were expressed as glucose uptake (% of control).

Dubey V., Mishra A.K, & Ghosh A.R. (2023). Appraisal of the Possible Role of PPARγ Upregulation by CLA of Probiotic Pediococcus pentosaceus GS4 in Colon Cancer Mitigation. PPAR Research, 2023, 9458308.

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Publication 2023

2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose 2-Deoxyglucose Biological Assay Caimans Cells Fluorescence Glucose

Top products related to «2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose»

2 nbdg by Thermo Fisher Scientific

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2-NBDG is a fluorescent glucose analog used as a tool for the detection and measurement of glucose uptake in cells. It serves as a substrate for glucose transporters and can be used to visualize and quantify cellular glucose utilization.

2 nbdg by Cayman Chemical

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2-NBDG is a fluorescent glucose analog used for the detection and measurement of glucose uptake in cells. It functions as a tool for studying glucose metabolism in biological systems.

2 nbdg by Merck Group

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2-NBDG is a fluorescent glucose analog that can be used to measure glucose uptake in cells. It is a non-radioactive, cell-permeable 2-deoxyglucose derivative that is labeled with the fluorescent dye 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose.

2 n 7 nitrobenz 2 oxa 1 3 diazol 4 yl amino 2 deoxyglucose 2 nbdg by Thermo Fisher Scientific

Sourced in United States

2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) is a fluorescent glucose analog used for the detection and measurement of glucose uptake in cells. It is a neutral, hydrophilic molecule that can be readily taken up by cells through glucose transporters.

Insulin by Merck Group

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Insulin is a lab equipment product designed to measure and analyze insulin levels. It provides accurate and reliable results for research and diagnostic purposes.

Fetal bovine serum (fbs) by Thermo Fisher Scientific

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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Glucose uptake cell based assay kit by Cayman Chemical

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The Glucose Uptake Cell-Based Assay Kit is a laboratory tool designed to measure glucose uptake in cells. It provides a quantitative method for analyzing cellular glucose absorption and metabolism.

Dimethyl sulfoxide (dmso) by Merck Group

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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.

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The FACSCalibur is a flow cytometry system designed for multi-parameter analysis of cells and other particles. It features a blue (488 nm) and a red (635 nm) laser for excitation of fluorescent dyes. The instrument is capable of detecting forward scatter, side scatter, and up to four fluorescent parameters simultaneously.

2 nbdg glucose uptake assay kit by Abcam

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The 2-NBDG glucose uptake assay kit is a fluorescence-based tool for the quantitative measurement of glucose uptake in cells. It utilizes the fluorescent glucose analog 2-NBDG to monitor glucose uptake activity. The kit provides all the necessary reagents and components to perform the assay.

What is 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose used for?

2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose is a fluorescent glucose derivative used to track glucose uptake and metabolism in biological systems. The nitrobenzoxadiazole group acts as a chromophore that can be detected using fluorescence microscopy or spectroscopy, allowing researchers to study glucose homeostasis and metabolic pathways in health and disease.

What are some common challenges in using 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose?

One potential challenge with using 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose is ensuring optimal labeling and detection conditions. Factors like cell permeability, background fluorescence, and subcellular localization can impact the effectiveness of this fluorescent probe. Careful optimization of experimental parameters is often required to obtain reliable and reproducible results.

Are there different variations or types of 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose?

Yes, there are different variations of 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose, such as different isomers or derivatives with modified linkers or substituents. These variations can have different physicochemical properties, fluorescence characteristics, and suitability for specific applications. Researchers should carefully evaluate the available options to select the most appropriate form for their experimental needs.

How can PubCompare.ai assist in the use of 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose?

PubCompare.ai can help researchers optimize their use of 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose in several ways. First, the platform allows you to efficiently screen the protocol literature to identify the most effective methods for using this fluorescent probe. Second, the AI-driven analysis provided by PubCompare.ai can help pinpoit critical nsights, such as the best experimental conditions or variations of the probe, to ensure reproducibility and accuracy in your research. By leveraging the power of PubCompare.ai, you can make more informed decisions and get the most out of your 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose experiments.

More about "2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose"

2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) is a synthetic glucose derivative that contains a nitrobenzoxadiazole moiety.
It is a valuable tool used for tracking glucose uptake and metabolism in biological systems.
The nitrobenzoxadiazole group serves as a chromophore that can be detected using fluorescence microscopy or spectroscopy, making it a useful marker for studying glucose homeostasis and metabolic pathways in health and disease. 2-NBDG is often utilized in conjunction with other related compounds and techniques, such as insulin, fetal bovine serum (FBS), glucose uptake cell-based assay kits, and dimethyl sulfoxide (DMSO).
These compounds and methods are commonly used in the analysis of glucose uptake and metabolism, often with the aid of flow cytometry instruments like the FACSCalibur.
The 2-NBDG glucose uptake assay kit is a popular tool that allows researchers to measure and quantify glucose uptake in cells, providing insights into cellular metabolism and energy production.
By optimizing your 2-NBDG research with AI-driven platforms like PubCompare.ai, you can enhance the reproducibility and accuracy of your studies, ensuring that you have access to the best protocols and products available.
Whether you're investigating glucose homeostasis, metabolic disorders, or other related topics, 2-NBDG and its associated techniques offer a powerful means of tracking and understanding glucose dynamics in biological systems.
By leveraging the insights and tools provided by PubCompare.ai, you can streamline your research and unlock new discoveries in this crucial area of study.