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2-(N-morpholino)ethanesulfonic acid

2-(N-morpholino)ethanesulfonic acid is a widely used buffer compound in biochemical and cell biology applications.
It maintains a stable pH in the physiological range and is compatible with a variety of experimental conditions.
PubCompare.ai can help researchers identify the most reliable and effective protocols from scientific literature, preprints, and patents to optimize experiments with this important reagent.
Our AI-driven comparisons can enhance reproducibility and accuary, ensuring your research with 2-(N-morpholino)ethanesulfonic acid is efficient and effective.

Most cited protocols related to «2-(N-morpholino)ethanesulfonic acid»

1
Synthesis and Characterization of RGD- and PEG-modified Alginate
Cited 8 times
Sodium alginate rich in guluronic acid blocks and with a high molecular weight (280 kDa, LF20/40) was purchased from FMC Biopolymer, and was prepared as has been described previously3 (link). Briefly, high molecular weight alginate was irradiated by a 3 or 8 Mrad Cobalt source to produce lower molecular weight alginates. RGD-alginate was prepared by coupling the oligopeptide GGGGRGDSP (Peptides International) to the alginate using carbodiimide chemistry at concentrations such that 2 or 20 RGD peptides were coupled to 1 alginate chain on average for high molecular weight alginate (peptide molar concentrations in low molecular weight alginates were kept the same according to high molecular weight alginate for each degree of substitution, respectively). For FRET experiments, either GGGGRGDASSK(carboxyfluorescein)Y or GGGGRGDASSK(Carboxytetramethylrhodamine)Y were used instead of standard RGD peptide sequence, and were coupled at a concentration of 2 peptides per alginate chain on average for high molecular weight alginate (peptide molar concentrations in low molecular weight alginates were kept the same according to high molecular weight alginate). The coupling efficiency using this procedure was previously characterized using 125I labeled RGD peptides3 (link). These correspond to densities of 150 μM and 1500 μM RGD in a 2% wt/vol alginate gel. Alginate was dialyzed against deionized water for 2–3 days (molecular weight cutoff of 3.5 kDa), treated with activated charcoal, sterile filtered, lyophilized, and then reconstituted in serum free DMEM (Life Technologies).
Polyethylene glycol (PEG)-alginate was prepared by coupling PEG-amine (5 kDa, Laysan Bio) to the low molecular weight alginate (35 kDa) using carbodiimide chemistry with a similar procedure to the RGD coupling3 (link). In brief, 295 mg of PEG-amine was mixed with 50 mL of 10 mg/mL alginate in 0.1 M MES (2-(N-morpholino)ethanesulfonic acid, Sigma-Aldrich) buffer at pH 6.5. Then 242 mg of EDC (N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, Sigma-Aldrich) and 137 mg of Sulfo-NHS (N-hydroxysulfosuccinimide, Thermo Fisher Scientific) were added into the solution. The reaction was carried out for 20 hours under constant stirring. The product was dialyzed against deionized water for 3 days (molecular weight cutoff of 10 kDa), filtered with activated charcoal, sterile filtered, and lyophilized. The structure of the PEG-alginate was confirmed with nuclear magnetic resonance (NMR) and gel permeation chromatography (GPC). Based on the change of molecular weight of alginate before and after PEG coupling (from 35 kDa to 45 kDa), an average of 2 PEG molecules were coupled to 1 alginate chain. This number was confirmed by 1H NMR spectroscopy (Supplementary Fig. S14).
Chaudhuri O., Gu L., Klumpers D., Darnell M., Bencherif S.A., Weaver J.C., Huebsch N., Lee H.P., Lippens E., Duda G.N, & Mooney D.J. (2015). Hydrogels with tunable stress relaxation regulate stem cell fate and activity. Nature materials, 15(3), 326-334.
Publication 2015
2-(N-morpholino)ethanesulfonic acid Alginate Alginates Amines Biopolymers Buffers Carbodiimides carboxyfluorescein Charcoal, Activated Cobalt Fluorescence Resonance Energy Transfer Gel Chromatography guluronic acid Magnetic Resonance Imaging Molar N-hydroxysulfosuccimide N-hydroxysulfosuccinimide Oligopeptides Peptides Polyethylene Glycols Serum Sodium Alginate Spectroscopy, Nuclear Magnetic Resonance Strains
2
Synthesis and Characterization of RGD- and PEG-modified Alginate
Cited 8 times
Sodium alginate rich in guluronic acid blocks and with a high molecular weight (280 kDa, LF20/40) was purchased from FMC Biopolymer, and was prepared as has been described previously3 (link). Briefly, high molecular weight alginate was irradiated by a 3 or 8 Mrad Cobalt source to produce lower molecular weight alginates. RGD-alginate was prepared by coupling the oligopeptide GGGGRGDSP (Peptides International) to the alginate using carbodiimide chemistry at concentrations such that 2 or 20 RGD peptides were coupled to 1 alginate chain on average for high molecular weight alginate (peptide molar concentrations in low molecular weight alginates were kept the same according to high molecular weight alginate for each degree of substitution, respectively). For FRET experiments, either GGGGRGDASSK(carboxyfluorescein)Y or GGGGRGDASSK(Carboxytetramethylrhodamine)Y were used instead of standard RGD peptide sequence, and were coupled at a concentration of 2 peptides per alginate chain on average for high molecular weight alginate (peptide molar concentrations in low molecular weight alginates were kept the same according to high molecular weight alginate). The coupling efficiency using this procedure was previously characterized using 125I labeled RGD peptides3 (link). These correspond to densities of 150 μM and 1500 μM RGD in a 2% wt/vol alginate gel. Alginate was dialyzed against deionized water for 2–3 days (molecular weight cutoff of 3.5 kDa), treated with activated charcoal, sterile filtered, lyophilized, and then reconstituted in serum free DMEM (Life Technologies).
Polyethylene glycol (PEG)-alginate was prepared by coupling PEG-amine (5 kDa, Laysan Bio) to the low molecular weight alginate (35 kDa) using carbodiimide chemistry with a similar procedure to the RGD coupling3 (link). In brief, 295 mg of PEG-amine was mixed with 50 mL of 10 mg/mL alginate in 0.1 M MES (2-(N-morpholino)ethanesulfonic acid, Sigma-Aldrich) buffer at pH 6.5. Then 242 mg of EDC (N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, Sigma-Aldrich) and 137 mg of Sulfo-NHS (N-hydroxysulfosuccinimide, Thermo Fisher Scientific) were added into the solution. The reaction was carried out for 20 hours under constant stirring. The product was dialyzed against deionized water for 3 days (molecular weight cutoff of 10 kDa), filtered with activated charcoal, sterile filtered, and lyophilized. The structure of the PEG-alginate was confirmed with nuclear magnetic resonance (NMR) and gel permeation chromatography (GPC). Based on the change of molecular weight of alginate before and after PEG coupling (from 35 kDa to 45 kDa), an average of 2 PEG molecules were coupled to 1 alginate chain. This number was confirmed by 1H NMR spectroscopy (Supplementary Fig. S14).
Chaudhuri O., Gu L., Klumpers D., Darnell M., Bencherif S.A., Weaver J.C., Huebsch N., Lee H.P., Lippens E., Duda G.N, & Mooney D.J. (2015). Hydrogels with tunable stress relaxation regulate stem cell fate and activity. Nature materials, 15(3), 326-334.
Publication 2015
2-(N-morpholino)ethanesulfonic acid Alginate Alginates Amines Biopolymers Buffers Carbodiimides carboxyfluorescein Charcoal, Activated Cobalt Fluorescence Resonance Energy Transfer Gel Chromatography guluronic acid Magnetic Resonance Imaging Molar N-hydroxysulfosuccimide N-hydroxysulfosuccinimide Oligopeptides Peptides Polyethylene Glycols Serum Sodium Alginate Spectroscopy, Nuclear Magnetic Resonance Strains
3
Histone Acetylation Detection Protocol
Cited 5 times
2-(N-morpholino)ethanesulfonic acid (MES) was purchased from EMD-Calbiochem (Gibbstown, NJ). Adenosine, adenosine 5′-diphosphoribose (ADP ribose), adenosine 5′-monophosphate (AMP), p-aminohippuric acid, 1-ethyl-3-(3-methylaminopropyl)carbodiimide (EDC), gluteraldehyde, hydroxylamine hydrochloride, nicotinamide, nicotinamide adenine dinucleotide (NAD+), nicotinamide mononucleotide (NMN), potassium phosphate dibasic, pyridine (99.8%), sodium azide, sodium cyanoborohydride, and sodium phosphate monobasic were obtained from Sigma-Aldrich Chemical Co. (Milwaukee, WI). N-Hydroxysulfosuccinimide (Sulfo-NHS) was from Pierce (Rockford, IL). Acetylated histone H3 (K9) peptide (H3K9Ac) and acetylated histone H3 (K14) peptide (H3K14Ac) were from Upstate/Millipore (Temecula, CA). Solutions were prepared using purified water from a Millipore MilliQ system (Millipore Corporation, Bedford, MA). BcMag amine-terminated magnetic beads (50 mg/mL, 1 µm) were purchased from Bioclone, Inc. (San Diego, CA). The manual magnetic separator Dynal MPC-S was from Invitrogen Corporation (Carlsbad, CA).
Yasuda M., Wilson D.R., Fugmann S.D, & Moaddel R. (2011). The Synthesis and characterization of SIRT6 protein coated magnetic beads: Identification of a novel inhibitor of SIRT6 deacetylase from medicinal plant extracts. Analytical chemistry, 83(19), 7400-7407.
Publication 2011
2-(N-morpholino)ethanesulfonic acid 4-Aminohippuric Acid Adenosine Adenosine Diphosphate Ribose Adenosine Monophosphate Carbodiimides Coenzyme I Exhaling Histone H3 Hydroxylamine Hydrochloride N-hydroxysulfosuccimide N-hydroxysulfosuccinimide Niacinamide Nicotinamide Mononucleotide Peptides potassium phosphate, dibasic pyridine Sodium Azide sodium cyanoborohydride sodium phosphate
4
DNA Labeling with Thiol-Reactive Nitroxide
Cited 5 times
A thiol-reactive nitroxide derivative, 3-iodomethyl-1-oxy-2,2,5,5-tetramethylpyrroline, was reacted with individual DNA strands that contain one phosphorothioate modification at a specific site. This reagent was prepared freshly from a precursor, 1-oxyl-2,2,5,5-tetramethyl-3-methane-sulfonyloxymethylpyrroline, following a published procedure (36 (link),38 ,39 (link)) (Supplementary Data). In the DNA labeling reactions, up to 0.1 mM of phosphorothioate modified DNA was mixed with 60–100 mM of 3-iodomethyl-1-oxy-2,2,5,5-tetramethylpyrroline in a mixture containing 100 mM of MES [2-(N-Morpholino)ethanesulfonic Acid (pH 5.8)] and 50% (v/v) formamide. After incubation in dark for 24 h at room temperature, excess nitroxide was removed using anion-exchange HPLC. The labeled DNA was desalted, lyophilized and stored as described above.
Cai Q., Kusnetzow A.K., Hubbell W.L., Haworth I.S., Gacho G.P., Van Eps N., Hideg K., Chambers E.J, & Qin P.Z. (2006). Site-directed spin labeling measurements of nanometer distances in nucleic acids using a sequence-independent nitroxide probe. Nucleic Acids Research, 34(17), 4722-4730.
Publication 2006
2-(N-morpholino)ethanesulfonic acid Anions formamide High-Performance Liquid Chromatographies Methane nitroxyl Sulfhydryl Compounds
5
Structural Determination of SARS-CoV PLpro
Cited 4 times

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Publication 2016
2-(N-morpholino)ethanesulfonic acid Diffusion Freezing Gel Chromatography Glycol, Ethylene lithium acetate Nitrogen polyethylene glycol 8000 Severe Acute Respiratory Syndrome Sodium Chloride Tromethamine

Most recents protocols related to «2-(N-morpholino)ethanesulfonic acid»

1
Protein Extraction and Western Blotting from Colonic Tissues
As previously described (Prigent et al., 2019 (link)), protein extraction from colonic tissues was performed with NucleoSpin RNA/Protein Kit (Macherey-Nagel, Hoerdt, France, Cat# 740966) according to the manufacturer instructions. Samples were further prepared for electrophoresis by diluting with a NuPAGE sample buffer (Life Technologies, Saint-Aubin, France, Cat# NP0008) then heated at 98°C for 5 min. Lysates were separated using the NuPAGE 4–12% Bis-Tris gels (Life Technologies, Cat# NP0336BOX) together with the 2-(N-morpholino)ethanesulfonic acid/sodium dodecyl sulfate running buffer (Life Technologies, Cat# IB23002) before electrophoretic transfer to nitrocellulose membranes (Life Technologies, Cat# NP0002) with the iBlot2 Dry Blotting System (Life Technologies, Cat# IB21001). Membranes were then blocked for 1 h at 21°C in Tris-buffered saline (Sigma, Cat# T5912) with 0.1% (v/v) Tween-20 (Sigma, Cat# P1379) and 5% (w/v) non-fat dry milk and incubated overnight at 4°C with the following primary antibodies: rabbit anti-GR (D8H2, 3660S 1:500, Cell Signaling, Danvers, MA, USA), mouse monoclonal anti-β-actin (1:10000; Sigma, Cat#A5441, RRID:AB_476744). Bound antibodies were detected with horseradish peroxidase-conjugated anti-rabbit (Life Technologies Cat# 31460, diluted 1:5000) or anti-mouse antibodies (Sigma, Cat# A9044, diluted 1:5000) and visualized by enhanced chemiluminescent detection (Biorad, Clarity ECL, Marnes-la-Coquette, France, Cat# 170-5061).
Blin J., Gautier C., Aubert P., Durand T., Oullier T., Aymeric L., Naveilhan P., Masson D., Neunlist M, & Bach-Ngohou K. (2023). Psychological stress induces an increase in cholinergic enteric neuromuscular pathways mediated by glucocorticoid receptors. Frontiers in Neuroscience, 17, 1100473.
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Publication 2023
2-(N-morpholino)ethanesulfonic acid Actins Anti-Antibodies Antibodies Bistris Buffers Colon Electrophoresis Gels Horseradish Peroxidase Milk, Cow's Mus Nitrocellulose Proteins Rabbits Saline Solution Sulfate, Sodium Dodecyl Tissue, Membrane Tissues Tween 20
2
Preparation of Amyloid-Beta Peptides
Ni(II) acetate and 2-(N-Morpholino)ethanesulfonic acid hydrate (MES) buffer were purchased from Sigma (Sigma/Merck KGaA, Darmstadt, Germany). The SDS detergent was bought from ICN Biomedicals Inc (USA). Sodium chloride and sodium hydroxide were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Wild-type (wt) Aβ(1–42) peptides, abbreviated as Aβ42, with the primary sequence DAEFR5HDSGY10EVHHQ15KLVFF20AEDVG25SNKGA30IIGLM35VGGVV40IA, were purchased synthetically manufactured from JPT Peptide Technologies (Germany), while recombinantly produced Aβ42 peptides were purchased from rPeptide LLC (USA). Recombinantly produced wild-type (wt) Aβ(1–40) peptides, abbreviated as Aβ40, as well as N-terminal truncated Aβ(4–40) peptides, were purchased as lyophilized powder from AlexoTech AB (Umeå, Sweden). The Aβ40 peptides were either unlabeled, uniformly 15N-labeled, or uniformly 13C,15N-labeled. A recombinantly produced mutant version of Aβ40, where the three histidine residues H6, H13, and H14 have been replaced with alanines, i.e. Aβ(1–40)(H6A, H13A, H14A) was also purchased from AlexoTech AB. This mutant is here abbreviated as Aβ40(NoHis). All Aβ variants were stored at − 80 °C until use, when they were dissolved to monomeric form before the measurements. The Aβ40 and Aβ(4–40) peptides were then dissolved in 10 mM NaOH to 100 µM concentration, and sonicated for 5 min in an ice-bath to dissolve possible pre-formed aggregates. Finally, buffer was added to the peptide solutions. All preparation steps were performed on ice, and the peptide concentrations were determined by weighing the dry powder and/or by NanoDrop measurements of dissolved material.
Berntsson E., Vosough F., Svantesson T., Pansieri J., Iashchishyn I.A., Ostojić L., Dong X., Paul S., Jarvet J., Roos P.M., Barth A., Morozova-Roche L.A., Gräslund A, & Wärmländer S.K. (2023). Residue-specific binding of Ni(II) ions influences the structure and aggregation of amyloid beta (Aβ) peptides. Scientific Reports, 13, 3341.
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Publication 2023
2-(N-morpholino)ethanesulfonic acid Acetate Alanine Bath Buffers Detergents Histidine Peptides Powder Sodium Chloride Sodium Hydroxide
3
Improved SDS-PAGE for Disulfide Bonds
Tris/MES SDS-polyacrylamide gel electrophoresis was chosen to confirm the presence of a disulfide bond between subunits in the mutant forms of RC [20 (link)], modified from [15 (link)]. It differs from conventional SDS polyacrylamide gel electrophoresis [21 (link)] by the addition of a higher percentage of polymer (acrylamide), the addition of MES (2-(N-morpholino)ethanesulfonic acid) (Sigma-Aldrich, St. Louis, MO, USA) to the buffer solution, and urea to the gels. These method alterations afford an increase in resolution. Otherwise, the protocol for setting up the experiment (polymerization of the gels, introduction of the samples, electrophoresis, staining, and washing of the gels) does not differ from the classical method. Comparatively mild denaturation conditions (30 °C, 60 min) for protein samples were used.
Selikhanov G., Atamas A., Yukhimchuk D., Fufina T., Vasilieva L, & Gabdulkhakov A. (2023). Stabilization of Cereibacter sphaeroides Photosynthetic Reaction Center by the Introduction of Disulfide Bonds. Membranes, 13(2), 154.
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Publication 2023
2-(N-morpholino)ethanesulfonic acid Acrylamide Buffers Disulfides Electrophoresis Gels Polymerization Polymers Proteins Protein Subunits SDS-PAGE Tromethamine Urea
4
Evaluation of α-Glucosidase, α-Amylase, and Lipase Inhibitors
α-Glucosidase (maltase from Saccharomyces cerevisiae), α-amylase (type VI-B, from porcine pancreas), lipase (type II, from porcine pancreas), p-nitrophenyl-α-D-glucopyranoside (PNPg), p-nitrophenol (PNP), 2-chloro-4-nitrophenyl-4-O-b-D-galactopyranosyl-a-D-maltoside (GalG2CNP), 2-chloro-4-nitrophenol (CNP), 4-methylumbelliferyl oleate (4-MUO), 4-methylumbelliferone (4-MU), orlistat, acarbose, pluronic F-127, Folin–Ciocalteu reagent, Na2CO3, Ca(OAc)2, NaCl, gallic acid, potassium phosphate buffer, phosphate-citrate buffer, chlorogenic acid, luteolin, oleuropein, piperine, absolute ethanol, and dimethyl sulfoxide (DMSO) were obtained from Merck/Sigma-Aldrich (Darmstadt, Germany). Chrysanthellin B was purchased from Extrasynthese (Genay, France). 2-(N-morpholino)-ethanesulfonic acid (MES) buffer was purchased from ThermoFisher Scientific (Waltham, Massachusetts, USA). NaN3 was obtained from Carlo Erba Reagents (Val-de-Reuil, France). Pure water was obtained using an Evoqua ultra-pure water production system (Water Technologies, Günzburg, Germany). The RED-NHS protein labeling kit was from NanoTemper Technologies (Munich, Germany).
TOTUM-63 (Batch No. V190033) was supplied as a powder by VALBIOTIS (Perigny, France). This product is formulated with five standardized plant extracts (Olea europaea L., Cynara scolymus L., Chrysanthellum indicum subsp. afroamericanum B.L.Turner, Vaccinium myrtillus L., and Piper nigrum L.). Voucher specimens of batches from TOTUM-63 and all plant extracts were deposited and stored in the VALBIOTIS sample library. Table 2 shows the phytochemical characterization of TOTUM-63. Total phenolic compound levels (in gallic acid equivalent) were assessed using the Folin–Ciocalteu colorimetric method [85 ] and a more precise characterization of phytochemical compounds was performed by HPLC-UV/Visible-MS using a 1200 LC system with a 6110 Single Quad MS-ESI detector (Agilent Technologies, Santa Clara, CA, USA) with a C18 Prodigy reversed-phase column (250 mm × 4.6 mm, 5 μm; Phenomenex, USA).
Haguet Q., Le Joubioux F., Chavanelle V., Groult H., Schoonjans N., Langhi C., Michaux A., Otero Y.F., Boisseau N., Peltier S.L., Sirvent P, & Maugard T. (2023). Inhibitory Potential of α-Amylase, α-Glucosidase, and Pancreatic Lipase by a Formulation of Five Plant Extracts: TOTUM-63. International Journal of Molecular Sciences, 24(4), 3652.
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Publication 2023
2-(N-morpholino)ethanesulfonic acid 2-chloro-4-nitrophenol 2-chloro-4-nitrophenyl-4-O-galactopyranosylmaltoside 4-methylumbelliferyl oleate 4-nitrophenol 4-nitrophenylgalactoside 11-dehydrocorticosterone Acarbose alpha Glucosidase Amylase Buffers cDNA Library Chlorogenic Acid Citrate Colorimetry Cynara scolymus Ethanol folin Gallic Acid Gene, THRA High-Performance Liquid Chromatographies Hymecromone Lipase Luteolin Olea oleuropein Orlistat Pancreas Phosphates Phytochemicals Pigs piperine Piper nigrum Plant Extracts Pluronic F-127 potassium phosphate Powder Prodigy Proteins Saccharomyces cerevisiae Sodium Azide Sodium Chloride Sulfoxide, Dimethyl Vaccinium myrtillus
5
Screening Phytotoxic Compounds against Dodder Seedlings
A screening of the 25 compounds (1–25) described in Figure 1 was performed to identify phytotoxic activity against the growth of C. campestris seedlings. The germination of C. campestris seeds is inhibited by a thick seed coat that preserves seedbank viability in agricultural fields over time [2 (link)]. To promote C. campestris germination, the hard seed coat was eliminated by scarification with sulfuric acid for 45 min [36 ], followed by thorough rinses with sterile distilled water. Then, twenty scarified C. campestris seeds were placed using tweezers onto 5 cm-diameter filter paper discs inside 5.5 cm-diameter Petri dishes. All compounds were dissolved in dimethyl sulfoxide and then diluted to 1, 0.5, and 0.25 mM in MES 0.3 mM (2-(N-Morpholino) ethanesulfonic acid) (Sigma M-8250). The final concentration of dimethyl sulfoxide in all treatments was 1%. This was conducted for all compounds except for compounds 3, 4, and 5, which were purchased in liquid form and dissolved directly into MES but supplemented with 1% of dimethyl sulfoxide to allow comparisons. Triplicate aliquots of 1 mL of each treatment were applied to filter paper discs containing the scarified C. campestris seeds. Triplicate aliquots of treatment only containing 1% of dimethyl sulfoxide and MES were used as a control. Treated C. campestris seeds were incubated in the dark at 23 °C for 5 days. The seedling length was measured in each of the five randomly chosen C. campestris seedlings for each of the three replicate filter paper discs per treatment. Seedling growth for each treatment was calculated in relation to the seedling growth of the corresponding control. In addition, notes were taken for each C. campestris seedling regarding whether the root apex had developed dark coloration. The percentage of seedlings that developed the darkening of root apices was calculated in each triplicated petri dish for each treatment.
Moreno-Robles A., Cala Peralta A., Zorrilla J.G., Soriano G., Masi M., Vilariño-Rodríguez S., Cimmino A, & Fernández-Aparicio M. (2023). Structure−Activity Relationship (SAR) Study of trans-Cinnamic Acid and Derivatives on the Parasitic Weed Cuscuta campestris. Plants, 12(4), 697.
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Publication 2023
2-(N-morpholino)ethanesulfonic acid DNA Replication fluoromethyl 2,2-difluoro-1-(trifluoromethyl)vinyl ether Germination Hyperostosis, Diffuse Idiopathic Skeletal Plant Embryos Plant Roots Seed Bank Seedlings Sterility, Reproductive Sulfoxide, Dimethyl sulfuric acid

Top products related to «2-(N-morpholino)ethanesulfonic acid»

1
2 n morpholino ethanesulfonic acid mes by Merck Group
Sourced in United States, Germany, United Kingdom, Australia, Sao Tome and Principe, China
2-(N-morpholino)ethanesulfonic acid (MES) is a chemical compound commonly used as a buffer in various laboratory applications. It is a zwitterionic organic compound that maintains a stable pH range, typically between 5.5 and 6.7, making it suitable for a variety of biological and biochemical experiments.
2
2 n morpholino ethanesulfonic acid by Merck Group
Sourced in United States, Germany, United Kingdom, Ireland, France, Australia
2-(N-morpholino)ethanesulfonic acid is a chemical compound used as a buffering agent in biochemical and cell culture applications. It is a zwitterionic organic compound with the chemical formula C₆H₁₃NO₄S. The compound helps maintain a stable pH environment in various laboratory procedures.
3
Bovine serum albumin (bsa) by Merck Group
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Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
4
N hydroxysuccinimide by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Canada, India, Poland, Sao Tome and Principe, France, Japan, Spain, Australia, Macao, Switzerland, Brazil, Sweden
N-hydroxysuccinimide is a chemical compound commonly used as an activating agent in organic synthesis. It is a stable, crystalline solid that can be used to facilitate the formation of amide bonds between carboxylic acids and primary amines. Its core function is to activate carboxylic acids, enabling their subsequent reaction with other functional groups.
5
Sodium chloride (nacl) by Merck Group
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NaCl is a chemical compound commonly known as sodium chloride. It is a white, crystalline solid that is widely used in various industries, including pharmaceutical and laboratory settings. NaCl's core function is to serve as a basic, inorganic salt that can be used for a variety of applications in the lab environment.
6
Sodium hydroxide (naoh) by Merck Group
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Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.
7
Hydrochloric acid (hcl) by Merck Group
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Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.
8
2 n morpholino ethanesulfonic acid hydrate mes by Merck Group
Sourced in United States
2-(N-Morpholino)ethanesulfonic acid hydrate (MES) is a buffering agent commonly used in biochemical and cell culture applications. It maintains a stable pH in the range of 5.5 to 6.7. MES is a zwitterionic compound that can effectively buffer solutions without significantly altering ionic strength.
9
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
10
Phosphate buffered saline (pbs) by Merck Group
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PBS (Phosphate-Buffered Saline) is a widely used buffer solution in biological and medical research. It is a balanced salt solution that maintains a stable pH and osmotic pressure, making it suitable for a variety of applications. PBS is primarily used for washing, diluting, and suspending cells and biological samples.

More about "2-(N-morpholino)ethanesulfonic acid"

2-(N-morpholino)ethanesulfonic acid, also known as MES, is a widely used buffer compound in biochemical and cell biology applications.
It is a zwitterionic organic compound that helps maintain a stable pH in the physiological range, typically between 5.5 and 6.7.
MES is compatible with a variety of experimental conditions, making it a versatile reagent for researchers.
MES is often used in conjunction with other commonly used compounds, such as bovine serum albumin (BSA), N-hydroxysuccinimide (NHS), sodium chloride (NaCl), sodium hydroxide (NaOH), and hydrochloric acid (HCl).
These substances can be used to prepare buffers, culture media, and other solutions for a range of experimental procedures.
Additionally, MES hydrate (MES,) and fetal bovine serum (FBS) are also important components in cell culture applications, where they provide a nutrient-rich environment for cell growth and maintenance.
Phosphate-buffered saline (PBS) is another commonly used buffer that can be used alongside MES to maintain physiological pH and ionic conditions.
PubCompare.ai, an AI-driven platform, can help researchers identify the most reliable and effective protocols from scientific literature, preprints, and patents to optimize experiments with MES.
By comparing various methods and techniques, the platform can enhance reproducibility and accuracy, ensuring your research with 2-(N-morpholino)ethanesulfonic acid is efficient and effective.