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3-amino-9-ethylcarbazole

3-amino-9-ethylcarbazole is a chemical compound with a wide range of applications in biological and medical research.
It is commonly used as a chromogenic substrate for the detection of peroxidase enzymes, making it a valuable tool in immunohistochemistry and other diagnostic assays.
The compound has also been investigated for its potential therapeutic properties, such as its ability to inhibit certain enzymes and modulate cellular processes.
Researchers can optimize their 3-amino-9-ethylcarbazole studies using PubCompare.ai, an AI-driven platform that enhances reproducibility and accuracy by helping to locate relevant protocols from literature, preprints, and patents, and providing AI-driven comparisons to identify the best protocols and products for their work.
By leveraging the power of AI, researchers can streamline their 3-amino-9-ethylcarbazole research process and enhance the overall quality and efficiency of their studies.

Most cited protocols related to «3-amino-9-ethylcarbazole»

1
Immunohistochemical Analysis of Tumor Angiogenesis
Cited 15 times
Six-micrometre thick serial sections of formalin-fixed, paraffin-embedded OSC and NDOLP tissue were deparaf-finized by the xylene-ethanol sequence. For antigen retrieval, the sections were microwaved at 500 W for 10 min., after which endogenous peroxidase activity was blocked with 3% hydrogen peroxide solution. Adjacent sections were incubated with monoclonal antibodies anti-TP (P-GF.44C Neo-Markers, Fremont, CA) diluted 1:100 for 1 hr at room temperature; anti-CD34 (QB-END 10; Bio-Optica, Milan, Italy) pan-endothelial marker diluted 1:50 for 1 hr at room temperature; anti CD68 (KP1; Dako) macrophage marker diluted 1:100 for 1 hr at room temperature [22, 28 (link)]. The bound antibody was visualized using biotinylated secondary antibody, avidinbiotin peroxidase complex, and 3-amino-9-ethylcarbazole or 3,3 diaminoben-zidine. Nuclear counterstaining was performed with Gill's haematoxylin number 2 (Polysciences, Warrington, PA) [18 ]. Primary antibody was omitted in negative controls.
TP expression was determined in five 400 × fields by the image analysis system (Quantimet 500 Leica) and TP positivity was evaluated on the basis of stained epithelial, macrophages and endothelial cells in terms of MVD [18, 24, 29 ]. Endothelial cells were identified as CD34- and TP-positive cells and MVD was evaluated in terms of both TP and CD34 immunostained vessels accordingly to Weidner's method with slight modifications [29 (link)]. Macrophages were identified as CD68- and TP-positive cells. Mean values ± standard deviation of epithelial cells, macrophages and MVD in both NDOLP and OSC was determined for each section and group of samples. Median value of epithelial cells, macrophages and MVD in OSC positive to TP was determined for each section and group of sample and was used as a cut-off to distinguish between high and low TP reactivity.
Ranieri G., Grammatica L., Patruno R., Zito A., Valerio P., Iacobellis S., Gadaleta C., Gasparini G, & Ribatti D. (2007). A possible role of thymidine phosphorylase expression and5-fluorouracil increased sensitivity in oropharyngeal cancer patients. Journal of Cellular and Molecular Medicine, 11(2), 362-368.
Publication 2007
3-amino-9-ethylcarbazole Anti-Antibodies Antigens Blood Vessel Cells Endothelial Cells Endothelium Epithelial Cells Ethanol Formalin Hematoxylin Immunoglobulins Macrophage Paraffin Embedding Peroxidase Peroxides Tissues Tritium Xylene
2
Nude Mice Xenograft Tumor Model
Cited 8 times
Animal experiments were undertaken in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals. BALB/c male nude mice aged of 8–12 weeks were used for this study. 5637 or H-bc cells were embedded in BD Matrigel Matrix (Becton, Dickinson, Franklin Lakes, NJ, USA)51 (link) and subcutaneously injected into the four sites at the back of mice as following: 1.7 × 107 cells/site for 5637, 0.7 × 107 cells/site for H-bc, 4 sites/mouse, 6 mice for 5637, 6 mice for H-bc, respectively. From the fourth day after cell injection, all 5637-generated tumors were intratumorally injected with 2nM miR-193a-3p agomiR or miR-193a-5p agomiR, while H-bc-generated tumors were injected with 4 nM miR-193a-3p/-5p/Mock antagomiR/PBS in a similar manner. From the sixth day after cell injection, three mice from 5637 and three from H-bc intraperitoneally received Pa (45 ug/ mouse) once in 2 days. The remaining six mice (three from 5637 and three from H-bc) received PBS as a mock treatment control. Mice were humanely killed on day 25, and the tumors were weighed and photographed. The tumor weight was described as the mean±S.D.
Expression levels of SRSF2, TP73 and Ki67 proteins were measured using immunochemical analysis on 5-mm slices of formalin-fixed paraffin-embedded tumor xenografts in nude mice. To avoid inter-treatment bias, the tissue slides from all the six groups were made on a single slide and subjected to the same immuno-staining simultaneously. Antigens were retrieved by pretreating dewaxed sections in a microwave oven at 750 Watts for 5 min in a citrate buffer (pH 6) processed with the Super Sensitive Link-Labeled Detection System (Biogenex, Menarini, Florence, Italy). The enzymatic activities were developed using 3-amino-9-ethylcarbazole (Dako, Milan, Italy) as a chromogenic substrate. Following counter staining with Mayer hematoxylin (Invitrogen), slides were mounted in an aqueous mounting medium (glycergel, Dako).Pictures were taken using the LEICA DM 4000B microscope (Wetzlar, Germany), while the relative level of each protein was calculated using the LEICA software (Wetzlar, Germany), and the percentage of the mock over the chemotherapeutic treated tumors was calculated and plotted.
Lv L., Deng H., Li Y., Zhang C., Liu X., Liu Q., Zhang D., Wang L., Pu Y., Zhang H., He Y., Wang Y., Yu Y., Yu T, & Zhu J. (2014). The DNA methylation-regulated miR-193a-3p dictates the multi-chemoresistance of bladder cancer via repression of SRSF2/PLAU/HIC2 expression. Cell Death & Disease, 5(9), e1402-.
Publication 2014
3-amino-9-ethylcarbazole Animals, Laboratory Antagomirs antibiotic H107 Antigens Buffers Cells Chromogenic Substrates Citrates enzyme activity Formalin Heterografts Males matrigel Mice, Inbred BALB C Mice, Nude Microscopy Microwaves Mus Neoplasms p73 protein, human Paraffin Embedding Pharmacotherapy Proteins Tissues
3
Immunohistochemical Profiling of Immune Markers
Cited 6 times
IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Serial sections (5-μm thick) were dewaxed and rehydrated. Heat-induced antigen retrieval was followed by endogenous peroxidase activity blockade with hydrogen peroxide. The sections were incubated overnight at 4°C with the following primary antibodies: CD207 (1:50; Atlas Antibodies), VE1 (1:50; Spring Bioscience), FOXP3 (1:75; Abcam), and PDL1 (1:50; R&D Systems). For antigen visualization, a peroxidase-labeled secondary antibody (EnVision/HRP system; DAKO) was used. Subsequently, the sections were rinsed in the kit buffer and immersed in diaminobenzidine stain. The expression of GATA3 (1:100; Cell Signaling Technology) and T-bet (1:50; Abcam) was performed via double IHC staining using DouMaxVision immunohistochemical double dye kits (KIT-9998; Maixin Biotech) according to the manufacturer's instructions, with 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium as the blue-black chromogen for alkaline phosphatase and 3-amino-9-ethylcarbazole as the red chromogen for horseradish peroxidase. The sections were counterstained with Harris' hematoxylin and then mounted. For the negative controls, phosphate-buffered saline was used instead of primary antibody.
Zeng K., Wang Z., Ohshima K., Liu Y., Zhang W., Wang L., Fan L., Li M., Li X., Wang Y., Yu Z., Yan Q., Guo S., Wei J, & Guo Y. (2016). BRAF V600E mutation correlates with suppressive tumor immune microenvironment and reduced disease-free survival in Langerhans cell histiocytosis. Oncoimmunology, 5(7), e1185582.
Publication 2016
3-amino-9-ethylcarbazole 5-bromo-4-chloro-3-indoxyl phosphate Alkaline Phosphatase Antibodies Antigens azo rubin S CD274 protein, human Formalin GATA3 protein, human Hematoxylin Horseradish Peroxidase Immunoglobulins Nitroblue Tetrazolium Paraffin Embedding Peroxidase Peroxide, Hydrogen Phosphates Saline Solution Stains
4
Quantification of RSV Infection in HEp2 Cells
Cited 6 times
We seeded 104 HEp2 cells in flat bottom 96 well plates (Costar). The next day we pre-incubated 100 TCID50 of RSV A2 or X virus mixed with B cell culture supernatant for 30 min at 37 °C before adding it in triplicate to HEp2 cells for 1 h at 37 °C. After 2 days we fixed the cells with 80% acetone and stained them with polyclonal RSV-HRP antibody (Biodesign) and 3-amino-9-ethylcarbazole for detection and visualization of RSV plaques by light microscopy (plaques were counted).
Kwakkenbos M.J., Diehl S.A., Yasuda E., Bakker A.Q., van Geelen C.M., Lukens M.V., van Bleek G.M., Widjojoatmodjo M.N., Bogers W.M., Mei H., Radbruch A., Scheeren F.A., Spits H, & Beaumont T. (2009). Generation of stable monoclonal antibody-producing BCR+ human memory B cells by genetic programming. Nature medicine, 16(1), 123-128.
Publication 2009
3-amino-9-ethylcarbazole Acetone Cell Culture Techniques Immunoglobulins Light Microscopy Senile Plaques Virus
5
Immunohistochemical Analysis of Tumor Tissues
Cited 5 times

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Publication 2017
3-amino-9-ethylcarbazole Antibodies Avidin azo rubin S Biotin Ethics Committees, Research HIF1A protein, human Homo sapiens Immunoglobulins Malignant Neoplasms Microarray Analysis Neoplasms Pathologists Peroxidase Tissues UBE2O protein, human Visually Impaired Persons

Most recents protocols related to «3-amino-9-ethylcarbazole»

1
Immunohistochemical Analysis of Atherosclerotic Plaques
Frozen sections were cut at a 5 μm thickness and mounted on microscope slides. The 5-μm-thick sections were stained with hematoxylin and eosin (H&E), and lipid deposits in the plaques were visualized by Oil Red O staining as previously described (3 (link), 15 (link)). Primary antibodies against CD66b, CD163, and CD68 cellular markets were diluted to 1:100, and a Histostain-Plus Kit AEC, Broad Spectrum (Invitrogen) was used for their detection. The sections were incubated with the primary antibodies for 2 hrs. at 37°C. Then, the sections were incubated with secondary antibody from the Histostain-Plus kit, for 30 min at 37°C. The 3-amino-9-ethylcarbazole (AEC) was used as a chromogen to detect the antibodies according to manufacturer’s instructions. Polymorphonuclear neutrophils (PMNs) were identified by the expression of CD66b, macrophages were identified by the expression of CD163 and foam cells were identified by the expression of CD68 scavenger receptors. Isotype controls were used as specified in the list of antibodies. Lipid deposits were stained with Oil Red O. Sequential sections were stained each for an antibody including for negative controls. At least 3 different sections were cut from the center of each plaque for each CD marker and in each section at least 5 different fields were analyzed.
Collagen and non-collagen proteins were detected by differential staining in tissue sections with two dyes - Sirius Red for all collagens and Fast Green for non-collagen proteins.
Lavie L., Si-on E, & Hoffman A. (2023). Giant phagocytes (Gφ) and neutrophil-macrophage hybrids in human carotid atherosclerotic plaques – An activated phenotype. Frontiers in Immunology, 14, 1101569.
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Publication 2023
3-amino-9-ethylcarbazole Antibodies azo rubin S CD163 protein, human CEACAM8 protein, human Cells Collagen Dyes Eosin Fast Green Foam Cells Frozen Sections Immunoglobulin Isotypes Immunoglobulins Lipids Macrophage Microscopy Neutrophil Proteins Scavenger Receptor Senile Plaques Tissues
2
mRNA and Protein Co-localization in Mouse Brain
A separate subset of mice from those used for in vitro electrophysiology measurements was deeply anesthetized with CO2 and then sacrificed by decapitation. Brain tissue was prepared and sectioned with a vibratome at 60 μm, as previously described (Singer et al., 2013a (link), 2016 (link)). mRNA and protein were co-localized on free-floating brain sections as previously described (Singer et al., 2013a (link)). In brief, following prehybridization for 1 h at 37°C, sections were incubated overnight with NO-GC, GC-A, Arc/Arg3.1, MR, or GR riboprobes at 56°C, incubated with anti-digoxigenin antibody conjugated to alkaline phosphatase (anti-Dig-AP, Roche, 11093274910), and developed as previously described (Singer et al., 2013a (link)). For protein detection, streptavidin–biotin was blocked according to the manufacturer’s instructions (Streptavidin–Biotin Blocking Kit, Vector Laboratories, Newark, CA, United States) after blocking endogenous peroxidase. Sections were incubated overnight at 4°C with the primary antibody against parvalbumin (Abcam, Berlin, Germany, anti-rabbit, 1:500, ab11427) as a marker for inhibitory neurons, followed by incubation with the secondary antibody (biotinylated goat anti-rabbit, Vector Laboratories, BA-1000) and chromogenic detection (AEC, 3-amino-9-ethylcarbazole, Vector Laboratories, SK-4200). For microscopy (BX61 microscope, Olympus, Hamburg, Germany) evaluation photographs of the hippocampus and auditory cortex were taken at a bregma position between −1.58 and −2.18 with a bright-field camera (DP 71, Olympus) for detection of mRNA and protein, without adjusting the picture frame or the plane of focus.
Calis D., Hess M., Marchetta P., Singer W., Modro J., Nelissen E., Prickaerts J., Sandner P., Lukowski R., Ruth P., Knipper M, & Rüttiger L. (2023). Acute deletion of the central MR/GR steroid receptor correlates with changes in LTP, auditory neural gain, and GC-A cGMP signaling. Frontiers in Molecular Neuroscience, 16, 1017761.
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Publication 2023
3-amino-9-ethylcarbazole Alkaline Phosphatase Auditory Area azo rubin S Biotin Brain Cloning Vectors Decapitation Digoxigenin Goat Immunoglobulins Mice, House Microscopy Neurons Parvalbumins Peroxidase Proteins Psychological Inhibition Rabbits Reading Frames RNA, Messenger Seahorses Singer Streptavidin Tissues
3
Immunohistochemical Analysis of Acss2 in Colon Cancer
We performed immunohistochemistry (IHC) on formalin-fixed and paraffin-embedded 5 μm sections from 34 paired stage I-IV human colon cancer and benign tissue samples mounted on poly-l-lysine–coated glass slides and collected by the University of Texas Southwestern Medical Center Tissue Management Core. Tumor and benign tissue samples were present on the same slide when undergoing IHC. We baked slides at 56°C for 30 min, deparaffinized in xylene, and then rehydrated through graded alcohols. We quenched endogenous peroxidase activity by incubating slides in 3% hydrogen peroxide (Cat. No. H1009-100ML, Sigma, Saint Louis, MO) in deionized water for 30 min and rinsing in deionized water twice for 5 min. For Acss2 staining, we submerged slides in BD Retrievagen A (pH 6.0) working solution (Cat. No. 550524, BD Biosciences, San Jose, CA) and heated to ~90°C in a microwave oven for 10 min, followed by slow cooling to room temperature (RT) over 60 min. We then rinsed slides twice with deionized water and once with PBS for 10 min. We blocked non-specific binding by a 60 min incubation with 10% goat serum (Cat. No. S-1000, Vector Laboratories, Burlingame, CA) in PBS. Next, we incubated slides overnight at 4°C with a rabbit monoclonal antibody directed against Acss2 (Cat. No. 3658, Cell Signaling Technology, Danvers, MA; 1:150 in 10% goat serum with PBS) and then 2 hr with biotin-conjugated goat anti-rabbit IgG (Cat. No. 111-065-045, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA; 1:500 in 10% goat serum with PBS). After rinsing in PBS, we incubated slides with HRP-conjugated streptavadin (Cat. No. 43–4323, Invitrogen Corporation, Camarillo, CA; 1:750 in 10% goat serum with PBS) for 60 min at RT. After rinsing in PBS, we visualized immunoreactive deposits by incubation with 3′-amino-9-ethylcarbazole (Cat. No. 00–2007, Invitrogen Corporation, Camarillo, CA). Finally, we counterstained slides with hematoxylin QS nuclear counterstain (Cat. No. H-3404, Vector Laboratories, Burlingame, CA) for 2 min and then coverslipped using Aqua-Poly/Mount Coverslipping Medium (Cat. No. 18606–20, Polysciences, Inc., Warrington, PA).
Garcia J.A., Chen R., Xu M., Comerford S.A., Hammer R.E., Melton S.D, & Feagins L.A. (2023). Acss2/HIF-2 signaling facilitates colon cancer growth and metastasis. PLOS ONE, 18(3), e0282223.
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Publication 2023
3-amino-9-ethylcarbazole ACSS2 protein, human Alcohols anti-IgG biotin 2 Cancer of Colon Cloning Vectors Formalin Goat Hematoxylin Homo sapiens Immunohistochemistry Lysine Microwaves Monoclonal Antibodies Neoplasms Paraffin Embedding Peroxidase Peroxide, Hydrogen Poly A Rabbits Serum Tissues Xylene
4
Multimodal Tissue Profiling of Mitochondrial DNA and Cytokeratins
In situ hybridization for mtDNA for both fresh frozen tissues and formalin-fixed paraffin-embedded (FFPE) tissues was performed based on a previous method (48 (link)). The tissue pretreatment conditions for human prostates was as previously described (48 (link)). For human colon FFPE tissues, the pretreatment was the same as that for human prostate FFPE tissues (48 (link)). For human pancreas FFPE tissues, the pretreatment was 15 min steaming in RNAscope® Target Retrieval reagent followed by 10 min incubation in protease plus at 40 °C.
The mtDNA in situ hybridization signals were developed using 3-amino-9-ethylcarbazole (AEC, Vector Laboratories, SK-4200), counterstained in hematoxylin and blued in Shandon bluing buffer (Thermo Scientific, 6769001). The slides were mounted, coverslipped, scanned, and de-coverslipped. The AEC was de-stained in an alcohol gradient. The slides were then rehydrated in an alcohol gradient and incubated using a HRP blocker (ACD bio) for 15 min at 40 °C before being subjected to sequential immunohistochemistry. This scanning, destaining and blocking process was repeated between all staining cycles.
After in situ hybridization, the slides were incubated with an anti-CK8 antibody (1:1500, Abcam, ab53280) for 45 min, PowerVision Poly-HRP anti-Rabbit IgG (Leica, PV6114) for 30 min, and visualized by incubation in AEC for 30 min, followed by counterstaining and bluing. For some slides, an additional CK903 IHC staining cycle was performed after CK8 detection. CK903 (1:50, Enzo, ENZ-C34903) was used as the primary antibody, PowerVision Poly-HRP anti-Mouse IgG (Leica, PV6119) as the secondary antibody, and AEC as the chromagen. All primary antibodies were diluted in Antibody Dilution Buffer (Ventana, adb250) unless specified.
The mtDNA in situ hybridization on mouse prostate tissues using Mm-mt-Co1 sense probe was performed as described previously (48 (link)). Hs-MYC mRNA in situ hybridization was performed similarly with probes against human MYC (ACD Bio, 311761).
Chen J., Zheng Q., Hicks J.L., Trabzonlu L., Ozbek B., Jones T., Vaghasia A., Larman T.C., Wang R., Markowski M.C., Denmeade S.R., Pienta K.J., Hruban R.H., Antonarakis E.S., Gupta A., Dang C.V., Yegnasubramanian S, & De Marzo A.M. (2023). MYC-driven increases in mitochondrial DNA copy number occur early and persist throughout prostatic cancer progression. bioRxiv.
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Publication Preprint 2023
3-amino-9-ethylcarbazole anti-IgG Antibodies Antibodies, Anti-Idiotypic Buffers Cardiac Arrest Cloning Vectors Colon DNA, Mitochondrial Ethanol Formalin Freezing Hematoxylin Homo sapiens Immunoglobulins Immunohistochemistry In Situ Hybridization Mus Pancreas Paraffin Peptide Hydrolases Poly A Prostate Prostatic Diseases Rabbits RNA, Messenger Technique, Dilution Tissues
5
Enumerating IgM Antibody-Secreting Cells
IgM antibody ASC were enumerated as described previously (Doucett et al., 2005 (link)). Briefly, 96-well ELISPOT plates (Multi-Screen HA Filtration, Millipore) were coated with 5 µg/ml anti-IgM (331), whole killed influenza PR8/34 A (400 HAU/ml; in house), Phosphorylcholine-BSA (Biosearch Technologies; 0.625 µg/ml), or 110 nm 1,2-dioleoyl-sn-glycero-3-phosphocholine liposomes (FormuMax, 10 mg/ml in 100% ethanol). Non-specific binding was blocked with 4% BSA in PBS. Tissues were processed as detailed above and cells were plated in culture media (RPMI 1640, 10% fetal bovine serum, 292 µg/ml L-glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin, and 50 µM 2-mercaptoethanol). Cells were twofold serially diluted and cultured overnight (∼16 h) at 37°C/5% CO2. Deionized water was used to lyse the cells after which IgM secretion was revealed with biotinylated anti-IgM (Southern Biotech), followed by streptavadin-horseradish peroxidase (Vector Labs), both diluted in 2% BSA in PBS. Last, a substrate solution (3.3 mg 3-amino-9-ethylcarbazole [Sigma-Aldrich] dissolved in dimethyl formamide and 0.015% hydrogen perioxide in 0.1 M sodium acetate was added and the reaction was stopped with deionized water. Spots were enumerated and images were captured with the AID EliSpot Reader System (Autoimmun Diagnostika).
Smith F.L., Savage H.P., Luo Z., Tipton C.M., Lee F.E., Apostol A.C., Beaudin A.E., Lopez D.A., Jensen I., Keller S, & Baumgarth N. (2023). B-1 plasma cells require non-cognate CD4 T cell help to generate a unique repertoire of natural IgM. The Journal of Experimental Medicine, 220(4), e20220195.
Publication 2023
2-Mercaptoethanol 3-amino-9-ethylcarbazole anti-IgM Cloning Vectors Culture Media Dimethylformamide Enzyme-Linked Immunospot Assay Ethanol Exanthema Fetal Bovine Serum Filtration Glutamine Glycerylphosphorylcholine Horseradish Peroxidase Hydrogen Immunoglobulin M Liposomes Penicillins Phosphorylcholine secretory IgM Sodium Acetate Streptomycin Tissues Virus Vaccine, Influenza

Top products related to «3-amino-9-ethylcarbazole»

1
3 amino 9 ethylcarbazole by Merck Group
Sourced in United States, Germany, United Kingdom
3-amino-9-ethylcarbazole is a chemical compound commonly used as a chromogenic substrate in laboratory procedures. It is a stable, crystalline solid that can be used to detect the presence of various enzymes or proteins through colorimetric reactions. The compound's core function is to serve as a substrate for these analytical tests, providing a visible color change when the target analyte is present. No further details on intended use are provided.
2
3 amino 9 ethylcarbazole aec by Merck Group
Sourced in United States, Germany
3-amino-9-ethylcarbazole (AEC) is a chemical compound used as a substrate in laboratory applications. It is commonly employed as a chromogenic detection reagent in various immunohistochemical and enzyme-linked immunosorbent assay (ELISA) techniques. AEC produces a colored reaction product when oxidized, which allows for the visualization and detection of target analytes.
3
3 amino 9 ethylcarbazole by Agilent Technologies
Sourced in Denmark, Italy, United States
3-amino-9-ethylcarbazole is a chemical compound commonly used as a chromogenic substrate in immunohistochemistry and enzymatic assays. It is a versatile and sensitive reagent that undergoes a color change reaction when exposed to certain enzymes, making it useful for the detection and visualization of target analytes in various laboratory applications.
4
Vectastain abc kit by Vector Laboratories
Sourced in United States, Canada, United Kingdom, Germany, Japan, France
The Vectastain ABC kit is a product by Vector Laboratories that is used for the detection of specific target antigens in tissue or cell samples. The kit includes reagents necessary for the avidin-biotin complex (ABC) method of immunohistochemistry. The core function of the Vectastain ABC kit is to provide a reliable and sensitive tool for the visualization of target molecules within a sample.
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3 amino 9 ethylcarbazole aec by Vector Laboratories
Sourced in United States
3-amino-9-ethylcarbazole (AEC) is a laboratory reagent commonly used as a chromogen in immunohistochemical and enzyme-linked immunosorbent assay (ELISA) applications. It produces a brown-colored reaction product when catalyzed by peroxidase enzymes, allowing for the visualization and detection of target molecules in biological samples.
6
3 amino 9 ethylcarbazole aec by Agilent Technologies
Sourced in United States, Germany, Denmark
3-amino-9-ethylcarbazole (AEC) is a chemical compound commonly used as a chromogenic substrate in various laboratory applications. It is a colorimetric reagent that produces a brown-red precipitate upon enzymatic reaction, often utilized in histochemical and immunohistochemical staining procedures.
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Mayer s hematoxylin by Merck Group
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Mayer's hematoxylin is a laboratory stain used in histology and cytology. It is a nuclear stain that selectively colors the nuclei of cells blue-purple. Mayer's hematoxylin is commonly used in the preparation of microscope slides for the examination of biological samples.
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X0909 by Agilent Technologies
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The X0909 is a laboratory instrument designed for analytical applications. It provides accurate and reliable measurements for various research and testing purposes. The core function of the X0909 is to perform precise analysis and data collection.
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3 amino 9 ethylcarbazole aec substrate by BD
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3-amino-9-ethylcarbazole (AEC) substrate is a chromogenic substrate used in immunohistochemistry and other applications to detect the presence of specific proteins or antigens. It produces a colored reaction product when catalyzed by peroxidase enzymes, allowing for the visualization of target molecules.
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Sk 4200 by Vector Laboratories
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The SK-4200 is a laboratory instrument designed for the detection and quantification of proteins. It utilizes a spectrophotometric method to measure the absorbance of samples, allowing for the determination of protein concentration.

More about "3-amino-9-ethylcarbazole"

3-amino-9-ethylcarbazole (AEC) is a versatile chemical compound with a wide range of applications in biological and medical research.
It is commonly used as a chromogenic substrate for the detection of peroxidase enzymes, making it a valuable tool in immunohistochemistry and other diagnostic assays.
The compound has also been investigated for its potential therapeutic properties, such as its ability to inhibit certain enzymes and modulate cellular processes.
Researchers can optimize their 3-amino-9-ethylcarbazole (AEC) studies using PubCompare.ai, an AI-driven platform that enhances reproducibility and accuracy by helping to locate relevant protocols from literature, preprints, and patents, and providing AI-driven comparisons to identify the best protocols and products for their work.
By leveraging the power of AI, researchers can streamline their 3-amino-9-ethylcarbazole (AEC) research process and enhance the overall quality and efficiency of their studies.
The Vectastain ABC kit is commonly used in conjunction with 3-amino-9-ethylcarbazole (AEC) for immunohistochemical staining, while Mayer's hematoxylin is often used as a counterstain.
The compound X0909, also known as 3-amino-9-ethylcarbazole (AEC) substrate, is another related product used for similar applications.
The SK-4200 kit is also a relevant tool for 3-amino-9-ethylcarbazole (AEC) based peroxidase detection and visualization.
By utilizing the insights and tools available, researchers can optimize their 3-amino-9-ethylcarbazole (AEC) studies, leading to more reproducibel and accurate results, and enhancing the overall quality and efficiency of their research.