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3-xylene

3-Xylene is a volatile, aromatic hydrocarbon compound with the molecular formula C8H10.
It is one of the three isomeric xylene compounds, along with o-xylene and p-xylene. 3-Xylene is commonly used as a solvent and in the production of other chemical compounds.
Researchers can leverage the PubCompare.ai platform to easily find and compaore protocols from literature, preprints, and patents related to 3-xylene, helping to identify the most accurate and reproducible experimental methods and ensuring reliable results in their 3-xylene reasearch.

Most cited protocols related to «3-xylene»

1
Bacterial Strains and Growth Conditions
Cited 14 times
The bacterial strains and plasmids used in this study are listed in Table 3. Bacteria were grown routinely in LB (10 g l-1 of tryptone, 5 g l-1 of yeast extract and 5 g l-1 of NaCl). E. coli cells were grown at 37°C while P. putida was cultured at 30°C. Selection of P. putida cells was made onto M9 minimal medium plates [55 ] with citrate (2 g l-1) as the sole carbon source. Antibiotics, when needed, were added at the following final concentration: ampicillin (Ap) 150 μg ml-1 for E. coli and 500 μg ml-1 for P. putida, kanamycin (Km) 50 μg ml-1 and chloramphenicol (Cm) 30 μg ml-1 for both species. 5-bromo-4-chloro-3-indolyl- β-D-galactopyranoside (Xgal) was added when required at 40 μg ml-1. The Pu-lacZ fusion of P. putida MAD1 (Table 3) was induced by exposing cells to saturating m-xylene vapors.
Martínez-García E., Calles B., Arévalo-Rodríguez M, & de Lorenzo V. (2011). pBAM1: an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes. BMC Microbiology, 11, 38.
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Publication 2011
3-xylene Ampicillin Antibiotics Bacteria Carbon Cells Chloramphenicol Citrates Escherichia coli Galactose Kanamycin LacZ Genes Plasmids Sodium Chloride Strains Yeast, Dried
2
Immunohistochemical Analysis of Renal Tissue
Cited 5 times
Renal sections were deparaffinized and hydrated in a descending series of ethanol (3× 5 min xylene, 2× 1 min 100% ethanol, 2× 1 min 96% ethanol, 1× 1 min 70% ethanol, aqua dest.). Endogenous peroxidase activity was blocked by incubating sections in 3% H2O2 for 15 min at room temperature. Antigen retrieval was performed in target retrieval solution (pH 6; Dako, Glostrup, Denmark) for 2.5 min at 120°C using a pressure cooker. Nonspecific binding sites were subsequently saturated in serum or blocked with avidin/biotin solution. All tissue sections were incubated with a primary antibody overnight at 4°C in appropriate dilutions (Table S2 in Supplementary Material). Sections were further processed using the VectaStain ABC kit (Vector Laboratories, Burlingame, CA, USA) according to the manufacturer’s recommendations and 3,3-diaminobenzidine (DAB) as substrate. Finally, the sections were counterstained in hemalaun, dehydrated, and mounted for observation. Immunohistochemistry images were acquired using an Olympus Bx60 microscope equipped with an XC30 camera at various magnifications. Images were taken after white balance, auto exposure, and introduction of scale bar using cellSens software (Olympus Deutschland, Hamburg, Germany). No further adjustments or signal amplifications were performed.
Dennhardt S., Pirschel W., Wissuwa B., Daniel C., Gunzer F., Lindig S., Medyukhina A., Kiehntopf M., Rudolph W.W., Zipfel P.F., Gunzer M., Figge M.T., Amann K, & Coldewey S.M. (2018). Modeling Hemolytic-Uremic Syndrome: In-Depth Characterization of Distinct Murine Models Reflecting Different Features of Human Disease. Frontiers in Immunology, 9, 1459.
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Publication 2018
3-xylene Antigens Avidin Binding Sites Biotin Cloning Vectors Ethanol Immunoglobulins Immunohistochemistry Kidney Microscopy Peroxidase Peroxide, Hydrogen Pressure Serum Technique, Dilution Tissues
3
Histological Evaluation of Vaginal Tissue
Cited 5 times
Histologic evaluation of vaginal tissues was used as the “gold standard” for determining estrous stage and was performed as previously described (Li and Davis, 2007 (link), Rendi et al., 2011 ). After 14 days of cytological evaluation of the estrous cycle, mice were euthanized immediately after cytological sample collection at predetermined assigned estrous stages. Euthanasia was performed by CO2 asphyxia and cervical dislocation. Vaginal tissue was surgically excised and fixed in 4% paraformaldehyde for a minimum period of 24 hours. Once fixed, the tissue was trimmed sagittally near the cervix so that the vaginal folds lateral to the cervix would be included in the tissue block. The tissues were then manually processed in the following manner: the tissues were incubated for 30 minutes each in 70%, 80%, 90%, 95% and twice in 100% ethanol, then 50%/50% and 70%/30% xylene/ethanol, and twice in 100% xylene. Tissues were then incubated for 90 minutes in 50%/50% xylene/paraffin at 60°C and for additional 90 minutes in pure paraffin at 60°C and were then embedded. Paraformaldehyde-fixed, paraffin-embedded tissue blocks were sectioned 4 µm thick, mounted on glass slides and manually stained with hematoxylin and eosin (Richard Allen) in the following manner: deparaffinization was achieved by drying the slides in an oven at 60°C for 20 minutes followed by incubation for 2 minutes 3 times in xylene, twice in 100% alcohol, once in 90% and 70% alcohol and once in running tap water. Slides were then incubated for 2 minutes in hematoxylin and washed under running tap water for 2 minutes. Slides were briefly dipped 4 times in acid alcohol [0.5% HCl in 70% EtOH] to remove excess hematoxylin and then washed under running water for 1 minute. Next slides were briefly dipped in ammonia water [0.1% ammonium hydroxide in H2O] and then washed under running water for 1 minute. Slides were then incubated twice in 95% ethanol for 1 minute, once in eosin for 30 seconds, twice in 95% ethanol for 1 minute, twice in 100% ethanol for 2 minutes, and 3 times in xylene for 2 minutes, followed by coverslipping.
Arnon G., Po-Ching L., Anne M B., Amy L M, & CheMyong K. (2014). Vaginal fold histology reduces the variability introduced by vaginal exfoliative cytology in the classification of mouse estrous cycle stages. Toxicologic pathology, 42(8), 1212-1220.
Publication 2014
3-xylene Acids Ammonium Hydroxide Asphyxia Cervix Uteri Eosin Estrous Cycle Estrus Ethanol Euthanasia Gold Hematoxylin Joint Dislocations Mus Neck Neoplasm Metastasis Operative Surgical Procedures Paraffin paraform Specimen Collection Tissues Vagina Xylene
4
Characterization of Organic Pollutant Profiles
Cited 4 times
Pesticide (alachlor, alpha-BHC, chlorpyrifos, and endrin ketone) and PAH (anthracene, benzo[ghi]perylene, chrysene, and fluoranthene) compounds were selected to represent a range physicochemical properties (Table 1). All were of purity ≥ 98% (Accustandard, USA). Tetrachloro-meta-xylene and PCB-209 (Accustandard, USA) were used as extraction surrogate standards for pesticides, and phenanthrene-d10, fluoranthene-d10, chrysene-d12, and benzo[ghi]perylene-d12 were used for PAHs (CDN Isotopes, Canada). Internal standards 4,4′-dibromooctafluorobiphenyl (Supelco Analytical, USA) and perylene-d12 (Chemservice, USA) were added immediately before instrumental analysis to correct for instrument variation (Table 2). Hexane solvents were Optima™ grade or better (Fisher Chemical, USA). PTFE transport/storage bags and Clip N Seal closures were purchased from Welch Fluorocarbon, Inc. (USA). LDPE lay-flat tubing used to make passive samplers was purchased from Brentwood Plastics, Inc. (USA). Average width of tubing is 2.7 cm, average membrane thickness is 75–95 μm, and average transient polymer cavity size is 10 Å (Anderson et al. 2008 (link)).
Donald C.E., Elie M.R., Smith B.W., Hoffman P.D, & Anderson K.A. (2016). Transport stability of pesticides and PAHs sequestered in polyethylene passive sampling devices. Environmental science and pollution research international, 23(12), 12392-12399.
Publication 2016
3-xylene alachlor anthracene Chlorpyrifos chrysene Dental Caries Endrin fluoranthene Fluorocarbons Hexanes Isotopes Ketones Perylene Pesticides phenanthrene Phocidae Polycyclic Hydrocarbons, Aromatic Polyethylene, Low-Density Polymers Polytetrafluoroethylene Solvents Surgical Clips Tissue, Membrane Transients
5
Comprehensive Soil Contaminant Analysis
Cited 3 times

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Publication 2019
3-xylene acenaphthene Acids ARID1A protein, human Benzo(a)pyrene Brominated Diphenyl Ethers Capillaries Chromatography chrysene Copper Desiccants fluorene Gas Chromatography-Mass Spectrometry Hydrocarbons Ions naphthalene Oxide, Aluminum Pesticides phenanthrene Place Cells Polychlorinated Biphenyls Polycyclic Hydrocarbons, Aromatic Retention (Psychology) Silicon Dioxide Solvents Sulfur Compounds

Most recents protocols related to «3-xylene»

1
Synthesis and Catalytic Evaluation of Mesoporous Materials
For the preparation of the mesoporous materials, USY Zeolite (CBV780) was supplied by Zeolyst, with a nominal Si/Al ratio of 40, and tetrapropylammonium bromide 98% was purchased from Sigma-Aldrich (St. Louis, MO). Sodium hydroxide (98% pellets) was supplied by Fluka.
For the synthesis of the quaternary ammonium bromide surfactant with the tripropyl head, tri-n-propylamine and 1-bromohexadecane both from Sigma-Aldrich were employed.
For the catalytic evaluation, 1,3,5-triisopropylbenzene (C15H24, 95%, Sigma-Aldrich) was used as feed stock. The chemicals used for GC calibration are:

From Sigma-Aldrich: Benzene (C6H6, ≥ 99.8%), toluene (C6H5CH3, ≥ 99.5%), para-xylene (C6H4(CH3)2, ≥99.5% GC), ortho-xylene (C6H4(CH3)2, ≥99.5% GC), meta-xylene (C6H4(CH3)2, ≥99.5% GC), 1,2,3-trimethylbenzene (C6H3(CH3)3, ≥99.5%, neat, GC), 1,2,4-trimethylbenzene (C6H3(CH3)3, 98%) and, 1,3-diisopropylbenzene (C12H18, 96%).

From Alfa Aesar: cumene (C9H12, 99%) and, 1,4-diisopropylbenzene (C12H18, 99%).

Mendoza-Castro M.J., Qie Z., Fan X., Linares N, & García-Martínez J. (2023). Tunable hybrid zeolites prepared by partial interconversion. Nature Communications, 14, 1256.
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Publication 2023
1-bromohexadecane 3-xylene 4-xylene ammonium bromide Benzene Bromides Catalysis cumene diisopropylbenzene Head o-xylene Pellets, Drug Sodium Hydroxide Surfactants tetrapropylammonium Toluene tripropylamine Zeolites
2
Ultrapure Water-Based Multimodal Analysis
Ultrapure water (18.2 MΩ
cm, ELGA Water purification system, Purelab Ultra MK 2, UK) was used
for all dilutions and washing steps. A multi-element stock solution
and single element standard solutions were purchased from Labkings
(Hilversum, The Netherlands). Bovine serum albumin (lyophilized powder,
BioReagent), Tris buffered saline (BioUltra), Triton X-100 (for molecular
biology), m-xylene (anhydrous, ≥99%), and ethanol (absolute,
EMSURE) were purchased from Sigma-Aldrich (Steinheim, Germany). The
target retrieval solution was bought from Agilent Technologies (Waldbronn,
Germany). Paraformaldehyde aqueous solution (Electron Microscopy grade,
16%) was obtained from Science Services (Munich, Germany) in form
of sealed ampoules, to ensure fresh solutions in each staining procedure.
The metal-conjugated antibodies used in this study (Table S3) and the Intercalator-Ir (Cell-ID, 125 μM)
were purchased from Fluidigm (San Francisco, CA, USA). LA-ICP-TOFMS
measurements were carried out in an ISO class 7 clean room. All cell
culture media and reagents were purchased from Sigma-Aldrich (Vienna,
Austria) and all plastic dishes, plates, and flasks from StarLab (Hamburg,
Germany) unless stated otherwise.
Schaier M., Theiner S., Baier D., Braun G., Berger W, & Koellensperger G. (2023). Multiparametric Tissue Characterization Utilizing the Cellular Metallome and Immuno-Mass Spectrometry Imaging. JACS Au, 3(2), 419-428.
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Publication 2023
3-xylene Antibodies Cells Electron Microscopy Ethanol Hyperostosis, Diffuse Idiopathic Skeletal Intercalating Agents Metals paraform Powder Saline Solution Serum Albumin, Bovine Technique, Dilution Triton X-100
3
Synthesis and Characterization of PTDMS
Poly(methylhydrosiloxane) with Mn = 1900 g/mol (ABCR, Karlsruhe, Germany), Karstedt’s catalyst (platinum complex of 1,3-divinyl-1,1,3,3-tetramethyldisiloxane in xylene, Sigma-Aldrich, St. Louis, MO, USA), 1-tetradecene (92%, Sigma-Aldrich, St. Louis, MO, USA), cross-linking agent 1,7-octadiene (98%, Sigma-Aldrich, Munich, Germany), and n-hexane (99%, Chimmed, Podolsk, Russia) were used for the synthesis of PTDMS. Helium (99.995%), methane (99.99%), ethane (99.95%), and n-butane (99.95%) were used for sorption and permeation measurements.
Malakhov A.O., Sokolov S.E., Grushevenko E.A, & Volkov V.V. (2023). Temperature Dependence of Light Hydrocarbons Sorption and Transport in Dense Membranes Based on Tetradecyl Substituted Silicone Rubber. Membranes, 13(2), 124.
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Publication 2023
1,3-butadiene 3-xylene Anabolism butane Ethane Helium Methane n-hexane Platinum Poly A
4
Characterization of Human Serum Albumin
5,5′-dithio-bis(2-nitrobenzoic acid) (DTNB), DL-homocysteine thiolactone (HTL) hydrochloride, and all solvents and other reagents, unless stated differently, were purchased from Sigma (St. Louis, MO, USA) at the highest available grade and used without purification. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay kit was purchased from Invitrogen (Waltham, MA, USA). Centricon concentrators with a 3 kDa molecular weight cut-off were purchased from Millipore. Perfluoro-m-xylene (PFX) was prepared and kindly provided by V.E. Platonov (Novosibirsk Institute of Organic Chemistry, SB RAS). Cyanine dye derivatives were obtained from Lumiprobe (Moscow, Russia). Sequencing-grade modified trypsin for MALDI ToF peptide analysis was purchased from Promega (Madison, WI, USA) (cat. no. V5111). Trypsin for protein trypsinolysis was obtained from Gibco (Carlsbad, CA, USA) (cat. no. 15090046). The human serum albumin (HSA) used in this study was purchased from Sigma–Aldrich (St. Louis, MO, USA) (cat. no. A3782). MS (MALDI ToF) m/z HSA 66.48 kDa. Ellman’s test (SH group content) indicated that HSA in this study contained 0.28 ± 0.05 sulfhydryl groups per protein molecule.
Mitin D.E, & Chubarov A.S. (2023). Fluorinated Human Serum Albumin as Potential 19F Magnetic Resonance Imaging Probe. Molecules, 28(4), 1695.
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Publication 2023
3-xylene Biological Assay Bromides derivatives Dithionitrobenzoic Acid homocysteine thiolactone hydrochloride Nitrobenzoic Acids Promega Proteins Sequence Analysis, Protein Serum Albumin, Human Solvents Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfhydryl Compounds Trypsin
5
Immunohistochemical Staining Protocol for L-FABP
For IHC staining, the following are the procedures: (a) deparaffinize sections, 3 changes of xylene, 10 min each; (b) re-hydrate in 2 changes of absolute alcohol, 5 min each; (c) 95% alcohol for 2 min; (d) 85% alcohol for 2 min; (e) 75% alcohol for 2 min; (f) wash 2 times in PBS buffer; (g) Hydrogen Peroxide Block to cover the sections for 10 min (Epredia, TL-125-QHD); (h) heat-mediated antigen retrieval: Tris-EDTA (pH9.0), 15 min; (i) Immunoblock, 5 min. (Epredia, TL-125-QHD); (j) primary antibody: L-FABP 1:500, 37 °C 1 h, wash 2 times in PBS buffer; (k) secondary antibody (Epredia, TL-125-QHD); (l) add 30 μl (1 drop) DAB Chromogen to 1.0 ml of DAB Buffer, mix by swirling and apply to tissue, 10 min (Epredia, TL-125-QHD); (m) counterstain in hematoxylin solution for 1 min, wash in running tap water 5 min; (n) dehydrate through 95% alcohol, 2 changes of absolute alcohol, 5 min each; (o) clear in 2 changes of xylene, 5 min each; and (p) mount with xylene-based mounting medium and then examined by light microscopy.
Chang C.C., Hsu C.C., Yu T.H., Hung W.C., Kuo S.M., Chen C.C., Wu C.C., Chung F.M., Lee Y.J, & Wei C.T. (2023). Plasma levels and tissue expression of liver-type fatty acid-binding protein in patients with breast cancer. World Journal of Surgical Oncology, 21, 52.
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Publication 2023
3-xylene 6H,8H-3,4-dihydropyrimido(4,5-c)(1,2)oxazin-7-one Absolute Alcohol azo rubin S Buffers Cardiac Arrest Edetic Acid Ethanol H antigen, bacterial Hematoxylin Immunoglobulins Light Microscopy Peroxide, Hydrogen Tissues Tromethamine Xylene

Top products related to «3-xylene»

1
M xylene by Merck Group
Sourced in United States, Germany, China
M-xylene is a colorless, flammable liquid chemical compound with the molecular formula C8H10. It is one of the three isomeric forms of xylene, which are widely used as solvents and in the production of other chemicals.
2
Toluene by Merck Group
Sourced in United States, Germany, Italy, United Kingdom, India, Spain, Japan, Poland, France, Switzerland, Belgium, Canada, Portugal, China, Sweden, Singapore, Indonesia, Australia, Mexico, Brazil, Czechia
Toluene is a colorless, flammable liquid with a distinctive aromatic odor. It is a common organic solvent used in various industrial and laboratory applications. Toluene has a chemical formula of C6H5CH3 and is derived from the distillation of petroleum.
3
Qiaamp dna ffpe tissue kit by Qiagen
Sourced in Germany, United States, United Kingdom, Netherlands, Japan, Spain, China, Italy, France, Australia, Canada
The QIAamp DNA FFPE Tissue Kit is a laboratory equipment designed for the purification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples. It is used to extract high-quality genomic DNA from FFPE samples for downstream applications such as PCR, sequencing, and other molecular biology techniques.
4
Ethylbenzene by Merck Group
Sourced in United States, Germany
Ethylbenzene is a clear, colorless liquid used as a chemical intermediate in the production of other compounds. It is commonly employed in the manufacturing of styrene, which is a key ingredient in various plastics and resins.
5
Acetonitrile by Merck Group
Sourced in Germany, United States, Italy, India, China, United Kingdom, France, Poland, Spain, Switzerland, Australia, Canada, Brazil, Sao Tome and Principe, Ireland, Belgium, Macao, Japan, Singapore, Mexico, Austria, Czechia, Bulgaria, Hungary, Egypt, Denmark, Chile, Malaysia, Israel, Croatia, Portugal, New Zealand, Romania, Norway, Sweden, Indonesia
Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.
6
3 isobutyl 1 methylxanthine by Merck Group
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3-isobutyl-1-methylxanthine is a chemical compound primarily used as a research tool in laboratories. It functions as a nonselective phosphodiesterase inhibitor, which can affect various cellular processes. The core function of this product is to serve as a laboratory reagent for scientific research purposes.
7
Recombinant human transforming growth factor β1 tgf β1 by Merck Group
Sourced in Italy
Recombinant human transforming growth factor-β1 (TGF-β1) is a laboratory reagent that is a member of the transforming growth factor beta family of cytokines. It is a protein that regulates various cellular processes, including cell growth, cell differentiation, and immune function.
8
Acetone by Merck Group
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Acetone is a colorless, volatile, and flammable liquid. It is a common solvent used in various industrial and laboratory applications. Acetone has a high solvency power, making it useful for dissolving a wide range of organic compounds.
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Mrs2179 by Merck Group
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MRS2179 is a laboratory reagent manufactured by Merck Group. It is a small molecule that can be used as a pharmacological tool in research applications. The core function of MRS2179 is to act as an antagonist for the P2Y1 receptor, which is involved in various physiological processes. Detailed information about its intended use or applications is not provided in this factual and unbiased description.
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Nf546 by Bio-Techne
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The NF546 is a laboratory instrument designed for the separation and analysis of proteins. It utilizes gel electrophoresis technology to separate proteins based on their molecular weight. The NF546 provides a reliable and consistent platform for protein separation and analysis in a variety of research and diagnostic applications.

More about "3-xylene"

3-Xylene, also known as m-xylene, is a volatile, aromatic hydrocarbon compound with the molecular formula C8H10.
It is one of the three isomeric xylene compounds, along with o-xylene (2-xylene) and p-xylene (4-xylene). 3-Xylene is commonly used as a solvent and in the production of other chemical compounds, such as toluene and ethylbenzene.
Researchers can leverage the PubCompare.ai platform to easily find and compare protocols from literature, preprints, and patents related to 3-xylene, helping to identify the most accurate and reproducible experimental methods and ensuring reliable results in their 3-xylene research.
This AI-driven platform allows researchers to streamline their 3-xylene studies by providing smart comparisons of protocols, ensuring that they are using the most effective and reproducible methods.
In addition to its use as a solvent, 3-xylene has also been studied for its potential biological and pharmacological applications.
For example, researchers have investigated the use of 3-isobutyl-1-methylxanthine, a derivative of 3-xylene, as a phosphodiesterase inhibitor, and the use of recombinant human transforming growth factor-β1 (TGF-β1) in combination with 3-xylene for tissue engineering applications.
Furthermore, 3-xylene is related to other aromatic hydrocarbons, such as M-xylene and toluene, which share similar chemical properties and applications.
Researchers may also find the QIAamp DNA FFPE Tissue Kit useful for extracting and purifying DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples containing 3-xylene.
Overall, the insights gained from the MeSH term description and the Metadescription can help researchers better understand the properties, applications, and research methods related to 3-xylene, and leverage the power of PubCompare.ai to streamline their 3-xylene research and ensure reliable results.