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8-anilino-1-naphthalenesulfonic acid

8-anilino-1-naphthalenesulfonic acid is a fluorescent dye used as a probe to study protein conformation and dynamics.
It binds to hydrophobic regions of proteins, causing a shift in its emission spectrum that can be used to monitor protein folding, unfolding, and ligand binding.
This versatile compound has applications in biochemistry, cell biology, and drug discovery reasearch.
Leverage PubCompare.ai's AI-driven platform to easily locate protocols and optimize your 8-anilino-1-naphthalenesulfonic acid experiments for maximal results.

Most cited protocols related to «8-anilino-1-naphthalenesulfonic acid»

1
Quantification of Glycerolipid Classes
Cited 5 times
Total glycerolipids were quantified from their fatty acids: in an aliquot fraction, 5 μg of 15:0 (internal standard) were added and the fatty acids present were converted to methyl esters (FAME) by a 1-hour incubation in 3 mL 2.5% H2SO4 in pure methanol at 100°C [29 (link)]. The reaction was stopped by addition of 3 mL water and 3 mL hexane. The hexane phase was analyzed by gas chromatography-flame ionization detector (GC-FID) (Perkin Elmer) on a BPX70 (SGE) column. FAMEs were identified by comparison of their retention times with those of standards (Sigma) and quantified using 15:0 for calibration. Extraction and quantification were performed at least 3 times. To quantify the various classes of neutral and polar glycerolipids, 300 μg of lipids were separated by thin layer chromatography (TLC) onto glass-backed silica gel plates (Merck) using two distinct resolving systems [30 (link)]. To isolate neutral lipids including DAG and TAG, lipids were resolved by TLC run in one dimension with hexane:diethylether:acetic acid (70:30:1, v/v). To isolate membrane glycerolipids, lipids were resolved by two-dimensional TLC. The first solvent was chloroform:methanol:water (65:25:4, v/v) and the second one chloroform:acetone:methanol:acetic acid:water (50:20:10:10:5, v/v). Lipids were then visualized under UV light, after spraying with 2% 8-anilino-1-naphthalenesulfonic acid in methanol, and scraped off the plate. Lipids were quantified by methanolysis and GC-FID directly from the scraped silica as described above.
Jouhet J., Lupette J., Clerc O., Magneschi L., Bedhomme M., Collin S., Roy S., Maréchal E, & Rébeillé F. (2017). LC-MS/MS versus TLC plus GC methods: Consistency of glycerolipid and fatty acid profiles in microalgae and higher plant cells and effect of a nitrogen starvation. PLoS ONE, 12(8), e0182423.
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Publication 2017
8-anilino-1-naphthalenesulfonic acid Acetic Acid Acetone Chloroform Esters Ethyl Ether Fatty Acids Flame Ionization Gas Chromatography Lipids Methanol n-hexane Retention (Psychology) Silica Gel Silicon Dioxide Solvents Thin Layer Chromatography Tissue, Membrane Ultraviolet Rays
2
Structural Analysis of Recombinant Human G6PD
Cited 5 times
Analysis of secondary structure of the recombinant human WT G6PD and the mutants enzymes was analyzed by CD in a spectropolarimeter (Jasco J-810®) equipped with a peltier thermostated cell holder [19 (link),23 (link),24 (link)]. Far-UV CD spectral scans at 25 °C were performed from 200 to 260 nm at 1 nm intervals in a quartz cell with a path length of 0.1 cm. The assays were conducted with a protein concentration of 0.2 mg/mL in 50 mM phosphate buffer at pH 7.35. Furthermore, CD measurements of recombinant human WT G6PD and the mutant’s enzymes were performed with 0.25 M of Gdn-HCl to evaluate if the loss activity in the time-curse inactivation assay was due to a wider structural disruption or local effect in the secondary structure. For both trials, the spectra of blanks were subtracted from those that contained the protein.
Analysis of conformational changes in the tertiary structure of recombinant human WT G6PD enzymes and mutants were evaluated by intrinsic fluorescence and their capacity to bind 8-anilinonaphthalene-1-sulfonate (ANS) assays. Both assays were performed in a Perkin-Elmer LS-55 fluorescence spectrometer (Perkin Elmer, Wellesley, MA, USA) as formerly reported [19 (link),23 (link)]. The fluorescence emission spectra from 310 to 500 nm were recorded after excitation at 295 nm, with an excitation and emission slits of 4.5 and 3.7 nm, respectively. All the assays of intrinsic fluorescence were conducted with a protein concentration of 0.1 mg/mL. ANS assays were performed in 25 mM phosphate buffer, pH 7.4 at 25 °C, using an excitation wavelength of 395 nm and recording emission spectra from 400 to 600 nm with an excitation and emission slits of 10 and 10 nm, respectively. The final concentrations of ANS and the recombinant human G6PD enzymes were the same as previously reported [19 (link),23 (link)]. For both spectroscopic assays, the spectra of blanks without protein were subtracted from the experimental samples that contained the respective protein.
Gómez-Manzo S., Marcial-Quino J., Vanoye-Carlo A., Serrano-Posada H., González-Valdez A., Martínez-Rosas V., Hernández-Ochoa B., Sierra-Palacios E., Castillo-Rodríguez R.A., Cuevas-Cruz M., Rodríguez-Bustamante E, & Arreguin-Espinosa R. (2016). Functional and Biochemical Characterization of Three Recombinant Human Glucose-6-Phosphate Dehydrogenase Mutants: Zacatecas, Vanua-Lava and Viangchan. International Journal of Molecular Sciences, 17(5), 787.
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Publication 2016
8-anilino-1-naphthalenesulfonic acid Alkanesulfonates ATP8A2 protein, human Biological Assay Buffers Cells Enzymes Fluorescence Glucosephosphate Dehydrogenase Homo sapiens Phosphates Proteins Quartz Radionuclide Imaging Spectrum Analysis Staphylococcal Protein A
3
Fluorescence assays of G6PD variants
Cited 4 times
Intrinsic fluorescence and 8-anilino-1-naphthalenesulfonic acid (ANS) assays of the recombinant human WT-G6PD and G6PD variants were conducted as described formerly [7 (link),21 (link)]. Both assays were monitored in a Perkin-Elmer LS-55 spectrofluorometer (Wellesley, MA, USA). Emission fluorescence spectra from 310 to 500 nm were recorded after excitation at 295 nm, whereas the ANS assays were conducted using an excitation wavelength of 395 nm and recording the emission fluorescence spectra from 400–600 nm. All the intrinsic fluorescence and ANS assays were conducted as described previously [7 (link),21 (link),25 (link)]. Background fluorescence from the buffer (blank), and buffer plus ANS was subtracted from those that contained the respective protein.
Gómez-Manzo S., Marcial-Quino J., Vanoye-Carlo A., Enríquez-Flores S., De la Mora-De la Mora I., González-Valdez A., García-Torres I., Martínez-Rosas V., Sierra-Palacios E., Lazcano-Pérez F., Rodríguez-Bustamante E, & Arreguin-Espinosa R. (2015). Mutations of Glucose-6-Phosphate Dehydrogenase Durham, Santa-Maria and A+ Variants Are Associated with Loss Functional and Structural Stability of the Protein. International Journal of Molecular Sciences, 16(12), 28657-28668.
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Publication 2015
8-anilino-1-naphthalenesulfonic acid Biological Assay Buffers Fluorescence Glucosephosphate Dehydrogenase Homo sapiens Proteins
4
Lipid Extraction and Analysis from P. pastoris
Cited 4 times
Lipids were extracted from freshly harvested P. pastoris cells according to a modified Folch method. The modification consists in boiling freeze-dried cells in ethanol for five minutes in order to denature endogenic enzymes such as phospholipases capable of damaging glycerolipids. The rest of the extraction followed the Folch procedure [30] (link).
Phospholipids were separated by two-dimensional thin layer chromatography (2D-TLC) using 20 cm×20 cm glass plates coated with silica (silica gel 60, Merck). The first chromatographic dimension was achieved by elution in chloroform∶methanol∶water (65∶25∶4, v/v), then the TLC plate was dried thoroughly under a stream of argon, and the second chromatographic dimension was performed in chloroform∶acetone∶methanol∶acetic acid∶water (50∶20∶10∶10∶5, v/v). Lipids were visualized under ultraviolet (UV) light after staining with 8-anilino-1-naphthalenesulfonic acid, 2% in methanol, and identified by comparison with standards.
Phospholipid spots were scraped off the TLC plates separately and known amounts of C21:0 fatty acid (Sigma) were added to each lipid spot as an internal standard. Fatty acids from the glycerolipids and the control C21:0 fatty acid were methylated by 1 h incubation at 100°C with 2.5% H2SO4 in pure methanol (3 mL total volume) in a sealed glass vial. Th reaction was stopped by the addition of 3 ml of water and 3 ml of hexane. Following the formation of a biphasic system, the upper phase containing the fatty acid methyl esters (FAMEs) was collected, dried under a stream of argon, re-suspended in pure hexane and then analyzed by gas chromatography with flame ionisation detector (GC-FID) (Perkin Elmer) on a BPX70 (SGE) column. The temperature program included 7 min 30 s at 130°C, then a ramp from 130°C to 180°C at 3°C/min and 10 min at 180°C. N2 was used as a carrier gas (3.5 mL/min). FAME retention times were compared with those of hydrogenated standard FAMEs (Sigma) and deuterated FAMEs. Deuterated FAMEs have a shorter retention time than the hydrogenated ones (figure S2). Standard deuterated C16:0 and C18:0 FAMEs were obtained by methanolysis of 1,2-dipalmitoyl(d62)-sn-glycero-3-phosphocholine and 1,2-distearoyl(d70)-sn-glycero-3-phosphocholine respectively (from Avanti) (figure S1).
de Ghellinck A., Schaller H., Laux V., Haertlein M., Sferrazza M., Maréchal E., Wacklin H., Jouhet J, & Fragneto G. (2014). Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells. PLoS ONE, 9(4), e92999.
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Publication 2014
8-anilino-1-naphthalenesulfonic acid A-130A Acetic Acid Acetone Argon Cells Chloroform Chromatography Enzymes Esters Ethanol Exanthema Fatty Acids Flame Ionization Freezing Gas Chromatography Glycerylphosphorylcholine Lipids Methanol n-hexane Phospholipase Phospholipids Retention (Psychology) Silica Gel Silicon Dioxide Thin Layer Chromatography Ultraviolet Rays
5
Fluorescence Spectroscopy of Enzyme Unfolding
Cited 3 times

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Publication 2006
8-anilino-1-naphthalenesulfonic acid Buffers Enzymes Fluorescence Luminescence NM 295 Tryptophan

Most recents protocols related to «8-anilino-1-naphthalenesulfonic acid»

1
Fluorescence Spectroscopy for HPC Hydrophobicity

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Publication 2023
8-anilino-1-naphthalenesulfonic acid Fluorescence Fluorescence Spectroscopy Kinetics Spectrum Analysis Titrimetry
2
CMC Determination of Chobimalt
The CMC of Chobimalt (Anagrade, CH220, purity > 99%; Mw = 1035 Da, Anatrace, OH, USA) was measured using the fluorescence intensity of a fluorescence probe 8-Anilino-1-naphthalenesulfonic acid (ANS, A1028; Sigma Aldrich, St. Louis, MO, USA). Chobimalt samples (150 µL) in concentrations from 0.05 µM up to 100 µM were mixed with 5 µL ANS (from a freshly prepared 5 mM stock solution, 5 mM in 50 mM PBS). Three different experimental approaches were applied and Chobimalt was dissolved in: (i) milli-Q water (denoted as H2O); (ii) acidic condition, pH 2.0 (adjusted using HCl, denoted as H2O-HCl); (iii) the presence of 100 mM NaCl at pH 2.0 (H2O-NaCl). The fluorescence intensity was measured using a 96-well plate by a Synergy MX (BioTek) spectrofluorometer. The excitation was set at 380 nm and the emission was recorded at 485 nm. The excitation and emission slits were adjusted to 9.0/9.0 nm and the top probe vertical offset was 6 nm. Each experiment was performed in triplicates; the error bars represent the average deviation for repeated measurements of three separate samples.
Artykulnyi O.P., Siposova K., Kriechbaum M., Musatov A., Almásy L, & Petrenko V. (2023). Micelle Formation in Aqueous Solutions of the Cholesterol-Based Detergent Chobimalt Studied by Small-Angle Scattering. Molecules, 28(4), 1811.
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Publication 2023
8-anilino-1-naphthalenesulfonic acid Acids Fluorescence Sodium Chloride
3
Fluorescence Characterization of Protein Aggregation
MedChemExpress (MCE) provided the CTZ (Monmouth Junction, NJ 08852, USA). Sigma-Aldrich provided HEWL, 8-anilino-1-naphthalenesulfonic acid (ANS), Thioflavin-T (ThT), and the rest of the chemicals (St. Louis, MO 68178, USA). All experimental solutions were made to an analytical grade in double-distilled water filtered with the Milli-Q system. The pH was measured with a SENTRON INTEGRATED SENSOR TECHNOLOGY 2001 pH meter (Texas City, TX, USA). Agilent Technologies Cary Eclipse-Fluorescence Spectrophotometer was used for all fluorescence measurements (Santa Clara, USA).
Altwaijry N., Almutairi G.S., Khan M.S., Shaik G.M, & Alokail M.S. (2023). Effect of Antihypertensive Drug (Chlorothiazide) on Fibrillation of Lysozyme: A Combined Spectroscopy, Microscopy, and Computational Study. International Journal of Molecular Sciences, 24(4), 3112.
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Publication 2023
8-anilino-1-naphthalenesulfonic acid Fluorescence thioflavin T
4
Protein Surface Hydrophobicity and Charge

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Publication 2023
8-anilino-1-naphthalenesulfonic acid Fluorescence Membrane Proteins Proteins Spectrometry, Fluorescence
5
Characterization of Yellow Pea Flour and Protein

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Publication 2023
8-anilino-1-naphthalenesulfonic acid Amylase Bacteria Biological Assay Biological Markers Chromatography Dialysis Flour Food Gels Glycine Glycoproteins Hydrophobic Interactions Laemmli buffer o-Phthalaldehyde octyl-sepharose CL-4B Pea Proteins Proteins Resins, Plant Stains Staphylococcal Protein A Sulfate, Sodium Dodecyl Tromethamine

Top products related to «8-anilino-1-naphthalenesulfonic acid»

1
8 anilino 1 naphthalenesulfonic acid ans by Merck Group
Sourced in United States, China, Germany
8-Anilino-1-naphthalenesulfonic acid (ANS) is a fluorescent dye used in various laboratory applications. It is a water-soluble compound that exhibits fluorescence properties when bound to certain biomolecules. The core function of ANS is to serve as a probe for studying the properties and interactions of proteins, lipids, and other biological molecules.
2
8 anilino 1 naphthalenesulfonic acid by Merck Group
Sourced in United States, Canada, China
8-anilino-1-naphthalenesulfonic acid is a chemical compound used in various laboratory applications. It functions as a fluorescent probe and has been utilized in the study of protein structure and dynamics.
3
Sodium chloride (nacl) by Merck Group
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NaCl is a chemical compound commonly known as sodium chloride. It is a white, crystalline solid that is widely used in various industries, including pharmaceutical and laboratory settings. NaCl's core function is to serve as a basic, inorganic salt that can be used for a variety of applications in the lab environment.
4
Cary eclipse fluorescence spectrophotometer by Agilent Technologies
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The Cary Eclipse Fluorescence Spectrophotometer is a laboratory instrument designed to measure the fluorescence properties of samples. It is capable of performing excitation and emission scans, as well as time-based measurements. The instrument uses a xenon flash lamp as the light source and provides high-sensitivity detection and rapid scanning capabilities.
5
Thioflavin t by Merck Group
Sourced in United States, Germany, Sao Tome and Principe, Canada, Italy, Japan, India, Australia, United Kingdom, France
Thioflavin T is a fluorescent dye used in the detection and quantification of amyloid fibrils. It exhibits enhanced fluorescence upon binding to these protein aggregates. The dye is commonly utilized in various research applications, including the study of protein misfolding and amyloidosis.
6
8 anilinonaphthalene 1 sulfonic acid ans by Merck Group
Sourced in United States
8-anilinonaphthalene-1-sulfonic acid (ANS) is a fluorescent dye used as a laboratory tool. It is a water-soluble compound that exhibits a shift in its fluorescence spectrum upon binding to various biological molecules, making it useful for structural and functional studies of proteins and other biomolecules.
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F 7000 by Hitachi
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The Hitachi F-7000 is a high-performance fluorescence spectrophotometer designed for versatile laboratory applications. It features advanced optics and a sensitive detector to provide accurate and reliable measurements of fluorescence intensity.
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8 anilinonaphthalene 1 sulfonic acid by Merck Group
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8-anilinonaphthalene-1-sulfonic acid is a fluorescent dye compound used in various laboratory applications. It serves as a probe for the investigation of protein structure and dynamics.
9
Bovine serum albumin (bsa) by Merck Group
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Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
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Sodium dodecyl sulfate (SDS) is a commonly used anionic detergent for various laboratory applications. It is a white, crystalline powder that has the ability to denature proteins by disrupting non-covalent bonds. SDS is widely used in biochemical and molecular biology techniques, such as protein electrophoresis, Western blotting, and cell lysis.

More about "8-anilino-1-naphthalenesulfonic acid"

8-anilino-1-naphthalenesulfonic acid (ANS) is a versatile fluorescent dye that has been extensively used in biochemistry, cell biology, and drug discovery research.
This compound, also known as 8-anilinonaphthalene-1-sulfonic acid, is a powerful tool for studying protein conformation, dynamics, and ligand binding.
ANS fluoresces weakly in aqueous solutions but exhibits a dramatic increase in fluorescence intensity and a blue shift in its emission spectrum when it binds to hydrophobic regions of proteins.
This property makes it a valuable probe for monitoring protein folding, unfolding, and interactions with other molecules, such as ligands or surfactants like sodium dodecyl sulfate (SDS).
The use of ANS in combination with techniques like fluorescence spectroscopy, such as the Cary Eclipse Fluorescence Spectrophotometer, has provided valuable insights into protein structure and function.
Additionally, ANS has been used as a fluorescent probe for the detection of amyloid-like aggregates, similar to the well-known dye Thioflavin T.
By leveraging the power of PubCompare.ai's AI-driven platform, researchers can easily locate protocols and optimize their ANS experiments for maximal results.
The platform's intuitive, typo-free interface allows users to streamline their workflow and access cutting-edge comparisons to identify the optimal protocols and products for their specific research needs.