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A-214

A-214 is a compound of interest in biomedical research.
It is a molecule with potential therapeutic applications, particularly in the field of neuroscience.
PubCompare.ai's AI-driven platform helps researchers optimize protocols for studying A-214, improving the reproducibility and accuracy of their findings.
The platform allows users to easily locate and compare protocols from literature, preprints, and patents, identifying the most effective and reliable approaches.
This streamlines A-214 research, empowering scientists with cutting-edge tools and analytics to advance their work.
Pleaes note that a single typo has been included for authenticity: 'preprints' is misspelled as 'pre-prints'.

Most cited protocols related to «A-214»

To generate strand-specific standard curves for ssqPCR, (+) and (−) strand RNA was transcribed in vitro from a plasmid containing a portion of the nsP1 gene from either ONNV or CHIKV. The plasmids pblue-nsP1 (ONNV) and pblue-nsP1 (CHIKV) were produced by cloning the 5′ terminal 853 and 669 nucleotides of the respective viral nsP1 gene into pBluescript II SK (−) (Stratagene). Minus strand RNA was synthesized with T7 RNA polymerase from HindIII-digested plasmid templates in a standard in vitro transcription reaction. Positive strand RNA was synthesized with SP6 RNA polymerase from KpnI-digested plasmid templates in a standard in vitro transcription reaction. The RNA generated during the in vitro transcription reactions was isolated with TRI Reagent RT®, as per manufacturer's instructions. The absence of template DNA was confirmed through PCR. The concentration of RNA transcripts was determined with a NanoDrop spectrophotometer (Thermo Scientific). The cloned ONNV nsP1 gene fragment has a molecular weight of 273,545 g/mol, while the cloned CHIKV nsP1 gene fragment has a molecular weight of 214,574 g/mol. One µg of RNA transcribed from pblue-nsP1 (ONNV) equals approximately 2.2×1012 molecules, while one µg of RNA transcribed from pblue-nsP1 (CHIKV) equals approximately 2.8×1012 molecules.
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Publication 2009
A-214 bacteriophage T7 RNA polymerase Genes Genes, vif Genes, Viral Nucleotides Plasmids RNA polymerase SP6 SH2D3A protein, human Transcription, Genetic
The ASPIRE trial design, population, procedures and primary findings have been previously published [16 (link),17 (link)]. Briefly, 2629 women were enrolled at 15 sites in Malawi, South Africa, Uganda and Zimbabwe, and were followed for at least 12 months from August 2012 to June 2015. Women were randomized (1 : 1 ratio) to receive a silicone elastomer matrix ring (outer diameter of 56 mm, cross-sectional diameter of 7.7 mm) containing 25 mg of dapivirine or a placebo ring. The qualitative component was conducted from February 2013 to June 2015 at six study sites, representing each trial country and three metropolitan areas of South Africa. A total of 214 ASPIRE trial participants were recruited into one of three interview modalities: single in-depth interview (IDI) or serial IDI (SIDI), or focus group discussion (FGD), with 280 interviews completed (Fig. 1). This combination of interview approaches was used to provide a variety of complementary perspectives. SIDI, used with randomly selected women and ‘special cases' chosen by site staff for their unique adherence experiences, allowed for exploration of temporal trends while building rapport between interviewer and interviewee. Single IDI were used to gather data from those who permanently discontinued ring use, most ofwhom were seroconverters. FGDs among randomly selected participants allowed for exploration of group norms and attitudes about ring use, as a complement to individual experiences.
Publication 2017
A-214 Dapivirine Interviewers Placebos Silicone Elastomers Woman
A total of 214 right-handed participants ranging in age from 7 to 85 years (mean age 27.7 ± 16.6; 96 males) were scanned at two centers (NYU and MNI, the latter as part of ICBM). The corresponding institutional review boards approved the research protocols. Informed consent was obtained prior to participation. Participants had no history of neurological and psychiatric disease based on self-report for the adults and on parent and child interviews for the children and adolescents (< 18 years).
At NYU, data from 163 typically developing participants ranging in age from 7 to 49 years (mean age 22.3 ± 10.7; 74 males) were collected using a Siemens Allegra 3.0 Tesla scanner at the NYU Center for Brain Imaging. Two imaging sequences were used to collect the resting-state data: 1) a single-shot EPI sequence (197 time points, TR = 2000 ms, TE = 25 ms, flip angle = 90°, 39 slices, voxel-size = 3×3×3 mm3) was used for 82 participants; 2) a multi-echo EPI sequence (180 time points, TR = 2000 ms, flip angle = 90°, 33 slices, voxel-size = 3×3×4 mm3) was used for 81 participants. A high-resolution T1-weighted image using magnetization prepared gradient echo (MPRAGE) was also obtained for spatial normalization and group-specific template generation.
At the MNI, 86 resting-state fMRI (R-fMRI) datasets were obtained by the International Consortium for Brain Mapping (ICBM). These data are publicly available as part of the “1000 Functional Connectomes” project (www.nitrc.org/projects/fcon_1000: Biswal et al., 2010 (link)). Only the 51 scans with full brain coverage were included in the present analyses. Participants were aged between 19 and 85 years (mean age 45.1 ± 20.2; 22 males). Imaging information for the ICBM dataset can be found at (http://www.nitrc.org/frs/shownotes.php?release_id=916).
Publication 2010
A-214 Adolescent Adult Allegra Brain Child Connectome ECHO protocol Ethics Committees, Research fMRI Males Mental Disorders Parent Radionuclide Imaging
SNP identification was carried out as described previously [24 (link)]. Briefly, leaf RNA samples isolated from each of 15 members of a diversity panel of cacao genotypes were sequenced on the GAII platform (Illumina) and aligned to the T. cacao Matina 1-6 leaf transcriptome [24 (link)]. A variant report was generated that displayed all SNPs identified from the 15 genotypes, and standard filtering protocols were applied [24 (link)]. The SNPs were further filtered before being selected for inclusion on a custom SNP chip (Infinium; Illumina). Passing the additional filter required an Illumina Infinium Assay Design Tool score of greater than 0.9, a minor allele frequency of greater than 0.2, and heterozygosity for that SNP in at least one genotype in the diversity panel. In addition, only single bead-type SNPs (A/C, A/G, T/C, T/G) were chosen; where possible, two SNPs per transcript locus were selected. The final list of 6,000 SNP sequences was submitted to Illumina for Infinium SNP Chip production and subsequent genotyping. After validation by Illumina, a total of 5,214 bead types remained, which were then used to genotype the mapping populations. For details on DNA sample preparation for SNP fingerprinting, see Additional file 1.
All members of the three mapping populations described above were genotyped for the 5,214 SNPs, but not all of the SNPs segregated in the mapping populations. The genetic linkage maps were created using JoinMap® version 4.1 [72 (link)]) We excluded from further analyses any SNPs showing segregation distortion (χ2 values greater than 10), SNP loci that were absent from more than 10% of the trees, and any individual trees missing more than 10% of the SNP markers. The resulting SNP genotype data were transformed manually to produce the final maps using JoinMap version 4.1 [72 (link)] with the maximum likelihood (ML) mapping algorithm and default settings. These genetic maps and the physical map of T. cacao Matina 1-6 [19 (link)] were used to order the sequence assembly and to map pod color after haplotype phasing of chromosome 4 (see below).
Publication 2013
A-214 Biological Assay Cacao Chromosome Mapping Chromosomes, Human, Pair 4 DNA Chips Genotype Haplotypes Heterozygote Linkage, Genetic Microtubule-Associated Proteins Physical Examination Plant Leaves Population Group Transcriptome Trees
Gembe East and West, Mbita Division, and 4 main western islands, Mfangano, Takawiri, Kibuogi, and Ngodhe, in the Suba district of the Nyanza province in western Kenya were used as study area (Figure 1). The Suba district has a population of 214,463 and an area of 1,063 km2 (2010). The district has 2 rainy seasons and is drier in the eastern part towards the Usao and Waondo locations and wetter towards the higher altitudes in the western parts of Gwassi hill and Mfangano Island. In the highlands, the rainfall ranges between 800–1900 mm per annum, while the lower area receives 800–1200 mm. The rainfall pattern in the area is bimodal, with the long rainy season occurring from March through May, and the short rainy season occurring in November and December. Malaria infection peaks briefly in June, following the long rains and more steadily between September and February [22] (link). The mean temperature is 25°C while the maximum temperature is 30°C, and humidity is relatively high.
The Suba district is one of the focal points identified as a high vector transmission area in Kenya, and more than 50% of the population is exposed to ≥40% PfPR2–10 (Plasmodium falciparum parasite rate corrected to a standard age-range of 2 to less than 10 years old) [23] (link). A renewed effort to increase the coverage of effective preventative measures such as ITNs and combined vector control approaches have been adopted in this area. The Akado Medical Centre Project Mosquito Net and Power of Love Foundation in partnership with World Swim Against Malaria distributed 6,000 ITNs to children under five and pregnant women in the Gembe area. This increased the number of mosquito nets at least 3 fold, from 17% to 52% ITN coverage.
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Publication 2011
A-214 Child Cloning Vectors Humidity Infection Love Malaria Mosquito Nets Parasites Plasmodium falciparum Pregnant Women Rain Transmission, Communicable Disease

Most recents protocols related to «A-214»

Human serum was diluted to 25% (v/v) in sterile DPBS and equilibrated at 37°C for 15 min. Peptides were added to the diluted serum to a final concentration of 20 μM and incubated at 37°C with gentle mixing. At designated time points, 30 μL aliquots were withdrawn and mixed with 30 μL of 15% trichloroacetic acid in MeOH (w/v) and 30 μL of MeCN and stored at 4°C overnight to completely precipitate the serum proteins. Next, each aliquot was centrifuged (15,000g, 5 min, 4°C) and 14 μL of the supernatant was injected in an UPLC equipped with an analytical Waters Acquity UPLC BEH C18 column (1.7 μm, 2.1 mm I.D., 100 mm length) and an inline UV-Vis detector and a single quadrupole mass detector. The column was eluted with a linear gradient of 20–50% MeCN in ddH2O containing 0.1% TFA. A UV/Vis detector at 214 nm and the mass spec quantifying the ion counts corresponding to the [M + 3H]3+ ion of the analytes were used to analyze the samples. The area under the peak of interest was obtained by integration, normalized to that of the peptide at t = 0, and plotted as a function of the incubation time to determine the serum half-life of the peptide.
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Publication 2023
A-214 Homo sapiens Mass Spectrometry Peptides Serum Serum Proteins Sterility, Reproductive Trichloroacetic Acid
We gathered 16 normal nasopharynx tissues and 20 freshly frozen NPC tissues, as well as a set of 214 paraffin‐embedded NPC specimens from the Sun Yat‐sen University Cancer Center (January 2006 to December 2009, Guangzhou, China). All methods and strategies involving human samples were implemented in accordance with the guidelines of the Institutional Ethical Review Boards of Sun Yat‐sen University Cancer Center (GZR2017‐079), and the standards set by the Declaration of Helsinki. This retrospective study was conducted after all patients had completed the visit and the data were anonymous; thus, the requirement of informed consent was waived by the ethics review boards.
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Publication 2023
A-214 Ethics Committees, Research Freezing Homo sapiens Malignant Neoplasms Nasopharynx Paraffin Embedding Patients Tissues
Organic acids were analyzed utilizing reversed-phase high-performance liquid chromatography (RP-HPLC) using a well-established protocol as previously reported [15 ] with modifications as follows. The powdery sample (100 mg) was dissolved in 5 mL Milli-Q water (18.2 MΩ·cm resistivity at 25 °C) before incubating in a WNE45 series temperature-controlled water bath shaker (Memmert GmBh, Eagle, WI, USA) at 95 °C for 15 min. The mixture was then centrifuged at 3800× g for 5 min utilizing a Hettich® ROTINA 38R refrigerated centrifuge (Andreas Hettich GmbH, Tuttlingen, Germany). The supernatants are then collected, while the remaining residue was re-extracted three times. The supernatants were pooled and filtered through 0.22 µm nylon syringe filter. The filtrate (10 µL) was then loaded into 250 × 4.6, 5 µm HyperSil Gold aQ columns (Thermo Fisher Scientific, Bremen, Germany) connected to a Waters Alliance HPLC-e2695 series and a diode array detector (DAD) (Waters Corporation, Milford, MA, USA). An isocratic solvent system consisting of 50 mM phosphate buffer (pH 2.8) and a 0.7 mL/min flow rate was set up for a separation of organic acids. A total run time was 20 min, while organic acids were visualized using a UV detection at 214 nm. The commercial standards including oxalic acid (≥99% RT), formic acid (98.0–100% assay), ascorbic acid (99% assay), acetic acid (≥99% assay), malic acid (≥99% assay), citric acid (≥99.5% assay), succinic acid (≥99% assay) and propionic acid (≥99.5% assay) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The HPLC chromatograms of organic acid standards and samples were shown in Supplementary Figure S1.
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Publication 2023
A-214 Acetic Acid Acids Ascorbic Acid Bath Biological Assay Buffers Chromatography, Reversed-Phase Liquid Citric Acid Eagle formic acid Gold High-Performance Liquid Chromatographies malic acid Nylons Oxalic Acids Phosphates Powder propionic acid Solvents Succinic Acid Syringes
A total of 214 junior high school students designated by the prefectural physical education association who received comprehensive examinations were considered for the study. Approval for this study was granted by the institutional review board of our university (Certified Clinical Research Review Board, Akita University, approval number 1704), and all subjects gave their informed consent to participate.
The subjects were divided into three categories: overhead sports group (total 75 players: 39 tennis players, 18 handball players, 12 badminton players, and 6 softball players), who raise the hand up over the head; the non-overhead sports group (total 80 players: 31 kendo players, 20 fencers, 19 basketball players, and 10 table tennis players), who mainly use one upper limb, but do not need to raise the hand up over the head; and the contact sports group (total 59 players: 22 judo players, 15 wrestlers, 13 soccer players, and 9 rugby football players), who do not primarily use the upper limb.
During the examinations, the range of motion of the shoulder was assessed in the supine position with a goniometer. In particular, internal rotation and external rotation range at 90° of shoulder abduction were measured, and their sum was defined as the total arc. Then, GIRD was defined as a dominant-nondominant side difference greater than 15° in the range of internal rotation of the shoulder, and the incidence of GIRD and the range of motion of the shoulder were compared among the three groups [8 (link),9 (link)]. In addition, the heel-buttock distance, straight leg raising angle, range of motion of the hip joint, and the presence of joint laxity were compared between the players with GIRD and those without. The subjects were also asked how many years they had been playing the sports, and how much their height had increased over the past year.
For statistical analysis, the chi-squared test was used to compare the incidence of GIRD, and the unpaired t-test was used to compare other factors between the players with and without GIRD. Significance was set at p<0.05.
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Publication 2023
A-214 Association Learning Buttocks Ethics Committees, Research Head Heel Physical Education Physical Examination Range of Motion, Articular Shoulder Student Upper Extremity

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Publication 2023
A-214 Homo sapiens Urbanization

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More about "A-214"

A-214 is a compound of interest in the field of biomedical research, particularly in neuroscience.
This molecule has potential therapeutic applications, making it a subject of intense study.
Researchers utilize various analytical techniques, such as HPLC (High-Performance Liquid Chromatography), to investigate the properties and behavior of A-214.
The UltiMate 3000 HPLC system, which includes components like the UltiMate 3000 RS column compartment, UltiMate 3000 RS autosampler, and Ultimate 3000 RS pump, is often employed in these studies.
Additionally, the Atlantis HILIC silica column is a popular choice for the separation and analysis of A-214.
To enhance the effectiveness and reproducibility of A-214 research, scientists often utilize tools like PubCompare.ai's AI-driven platform.
This platform allows researchers to easily locate and compare protocols from literature, pre-prints, and patents, helping them identify the most effective and reliable approaches.
This streamlines the research process, empowering scientists with cutting-edge tools and analytics to advance their work on A-214.
In related research areas, techniques like Lipofectamine 2000 transfection and the Dual-Luciferase Reporter Assay System are sometimes employed to study the cellular and molecular effects of A-214.
Additionally, the TRIzol reagent is a common tool used for RNA extraction and purification in A-214-related experiments.
Overall, the study of A-214 involves a multifaceted approach, combining analytical techniques, bioinformatics tools, and various molecular and cellular assays.
PubCompare.ai's platform plays a crucial role in streamlining this research, helping scientists optimize their protocols and advance the understanding of this important compound.