The largest database of trusted experimental protocols

Acetamiprid

Acetamiprid: A Powerful Insecticide for Crop Protection.
Acetamiprid is a systemic neonicotinoid insecticide used to control a wide range of sucking and chewing insects on various crops.
This AI-powered platform helps researchers optimize Acetamiprid research by providing access to the best experimental protocols from literature, preprints, and patents.
Leveraging advanced AI comparisons, the platform enhances reproducibility and accuracy, ensuring your Acetamiprid experiments deliver reliable, high-quality results.
Experience the power of AI-driven research optimization and take your Acetamiprid studies to new heights.

Most cited protocols related to «Acetamiprid»

Three German cockroach strains were used. The Johnson Wax strain (JWax-S) has been maintained in the laboratory for >80 yr without insecticide exposure and was used as a standard susceptible strain. Two field strains were collected from multifamily housing sites in Danville, IL (D-IL strain), and Indianapolis, IN (I-IN strain), during December 2014 and March 2015. Full human subjects research approval was granted by the Purdue University Institutional Review Board (Protocol number 1411015460R001). The field strains were collected from multiple apartments across each site and pooled to establish laboratory “meta” populations. These populations were maintained without insecticide selection pressure. Colonies were reared in Ziploc plastic containers (44.3 by 30 by17 cm3/15.14 liter; S.C. Johnson Inc., Racine WI, USA) with screened lids and held in a controlled-environmental chamber at 26 ± 1 °C and a photoperiod of 12:12 (L:D) h. Cardboard for shelter, rodent diet (number 8604; Harlan Teklad, Madison, WI), and water were provided ad libitum to the rearing boxes. Bioassay experiments were done with 1–2-wk-old adult males. To obtain enough adult males of appropriate age for experiments, rearing containers were established with gravid adult females and mixed-age nymphs. Adult male cockroaches were separated out of these containers at the beginning of every week and aged for an additional week before using in insecticide bioassays. All bioassays with field strains were performed after three to four generations (within 12 mo after collection).
Technical grade gel bait and spray product AIs used in vial bioassays were purchased from ChemService (West Chester, PA), Fisher Scientific (Pittsburgh, PA), or Sigma-Aldrich (St. Louis, MO). These AIs included indoxacarb (99.1% purity), abamectin (98.3%), boric acid (99.9%), beta-cyfluthrin (99.5%), bifenthrin (99%), lambda-cyhalothrin (99.5%), fipronil (98.3%), dinotefuran (98.4%), imidacloprid (99.4%), acetamiprid (99.5%), clothianidin (99.5%), thiamethoxam (99.5%), chlorfenapyr (99.1%), and hydramethylnon (99.5%). These AIs were selected because they are currently registered for use in cockroach control products. Three cockroach gel baits, InVict Gold (imidacloprid 2.15%; Rockwell Lab Ltd, Kansas City, MO), Maxforce Professional Insect Control (hydramethylnon 2.15%; Bayer, Research Triangle Park, NC), and Magnetic (boric acid 33.3%; Nisus Co., Rockford, TN) were purchased from Univar (Indianapolis, IN) for follow-up testing in no-choice feeding bioassays.
Publication 2017
abamectin acetamiprid Adult ARID1A protein, human beta-cyfluthrin bifenthrin Biological Assay boric acid chlorfenapyr clothianidin Cockroach, German Cockroaches Diet dinotefuran Ethics Committees, Research fipronil Gold hydramethylnon imidacloprid indoxacarb Insect Control Insecticides lambda-cyhalothrin Males Nymph Population Group Pressure Rodent Strains Thiamethoxam Woman
Pesticide residues were extracted from honey bee pollen using a modified QuEChERS method59 (link). A total of 15 g of pollen from each hive, on each sample date, was homogenized and separated into three 50-ml centrifuge tubes and extractions were performed on three replicate pollen samples of 5 g each. Sufficient levels of honey bee pollen were not available for collection due to disturbance of the hives by vertebrate predators (probably striped skunks, Mephitis mephitis) on the following sample dates: Hive 1A (26 July), Hive 1B (24 May), Hive 2A (24 May, 2 August through 13 September) and Hive 2B (24 May). These ten sampling points were excluded from calculations of seasonal pesticide prevalence in pollen samples. Therefore, over the 16-week sampling period a total of 30 pollen samples were collected from colonies at the non-agricultural area, 24 samples from the colonies adjacent to the untreated maize field and 32 samples from the colonies adjacent to the neonicotinoid-treated maize field, for a grand total of 86 pollen samples across all sites. We added 30 ml of extraction solution (15 ml of 1% dH2O/acetic acid solution + 15 ml acetonitrile) to each tube and mixed thoroughly, followed by the addition of 6 g of anhydrous magnesium sulfate (MgSO4) and 1.5 g sodium acetate (NaAc). Tubes were mixed gently on an agitator for 10 min and then centrifuged. Fifteen millilitres of supernatant was removed and dispensed into a 15-ml Agilent dispersive Solid Phase Extraction tube containing 400 mg primary secondary amine, 400 mg C18 and 1,200 mg MgSO4. Two millilitres of toluene was added to each 15 ml Agilent tube, to aid in the extraction of planar pesticides from the pollen matrix60 . Tubes were gently agitated, centrifuged and 200 μl of the supernatant from this final step of processing was transferred to 96-well plates for analysis by liquid chromatography (LC) and tandem mass spectrometry at the Bindley Bioscience Center at Purdue University. An Agilent Zorbax SB-Phenyl 4.6 × 150 mm, 5 μm column was used for LC separation (Agilent Technologies, Santa Clara, CA) and an Agilent 1200 Rapid Resolution LC system coupled to an Agilent 6460 series triple quadrupole mass spectrometer was used to identify pesticide residues based on retention time and co-chromatography with high-purity analytical standards of the 65 pesticide targets evaluated in our study (Table 7). Analytical-grade standards were purchased from Sigma-Aldrich, Accustandard and Fisher Scientific. We used deuterated internal standards to quantify the concentrations of the neonicotinoids acetamiprid, clothianidin, imidacloprid and thiamethoxam in pollen samples. Ten microlitres of a spiking solution containing only deuterated internal standards of these four neonicotinoids was added to 190 μl of each pollen sample for a total volume of 200 μl for analysis on the LC and tandem mass spectrometry instrument. A stock mixture of the 61 remaining analytical standards was created and from that mixture a series of 8 serial dilutions were conducted and analysed on the instrument to establish a standard curve to which pesticide residues in pollen samples was calibrated to determine final concentrations. The Agilent MassHunter METLIN Metabolite Personal Compound Database and Library61 was used to identify compounds based on known ratios of parent mass and at least two fragment transitions. The final concentration of pesticide residues was calculated by averaging the values detected from the three replicate 5 g pollen samples processed from each hive on each available date.
Full text: Click here
Publication 2016
In all experiments, mortality was tallied after 48 hr. Control mortality was <5%. Average control mortality was 2.7%. At least 6 doses of each pesticide plus a control (water only) were tested in each replication per pesticide per bee species. Each replication included a total of 60–135 bees of each species depending on species' availability. Dose-mortality regressions were estimated assuming the normal distribution (i.e., probit model) with the computer program PoloPlus [47] as described by Robertson et al. [48] . We used a two-step procedure to analyze data for each chemical. In the first step, we examined plots of standardized residuals for outliers, which were then eliminated from the data sets. The second and final probit analysis was done to test hypotheses of parallelism (slopes not significantly different) and equality (slopes and intercepts not significantly different) with likelihood ratio tests [48] . PoloPlus also calculated lethal dose ratios (LDR's) of the most toxic chemical compared with all other chemicals for each species. An LDR provides a means to test whether two LD's are significantly different (i.e., when the 95% CI for the LDR did not include the value 1.0 [47] , [48] ).
For tests with a mixture, at least 5 doses of the mixture that bracketed 5–95% mortality were tested concurrently with experiments with at least 5 doses of individual mixture components. As before, 60–135 bees of each species were tested depending on species' availability. To test the hypothesis of independent joint action of fenbuconazole with acetamiprid or imidacloprid, we used the computer program PoloMix [49] . Assuming independent joint action of two mixture chemicals, test subjects can die of three possible causes. The first cause is natural mortality, with a probability po (a constant). The other two causes of mortality are the probabilities of mortalities for chemical 1 or chemical 2. For the first chemical, the probability of response (p1) is a function of dose D1. Usually, the probit or logit of dose X of chemical 1 is log(D1) (i.e., X1 = log[D1]). For the second chemical, the probability of response (p2) is a function of dose D2. If these three causes of mortality are independent, the probability of death (p) is p = p0+(1−p0)p1+(1−p0)(1−p1) p2. When each “+” sign means “or” and each product means “and,” this equation means that the total probability of death equals death from natural causes (p0), or no death from natural causes (1−p0) and death from the first chemical [e.g., (1−p0)p1], or no death from natural causes or from the first chemical [i.e., (1−p0)(1−p1)], but death from the second chemical [i.e., (1−p0)(1−p1) p2]. The χ2 statistic produced by PoloMix [49] was used to test the hypothesis of independent joint action. This test statistic is calculated by obtaining an estimate for the probability of mortality (p) for several dose levels of the two components and then comparing (the estimate of p) with the observed proportion killed at the corresponding dose levels. The three contributions to p are estimated separately. First, p0 is calculated as the proportional mortality observed in the control group. Next, p1 and p2 are estimated from bioassays of chemical 1 and chemical 2, with test statistics estimated from PoloPlus [47] .
Full text: Click here
Publication 2013
acetamiprid Bees Biological Assay Chemical Actions DNA Replication fenbuconazole imidacloprid Joints Pesticides Poisons TEST mixture
Eggs, nymphs, and pupae were collected from leaves of collard plants (Brassica oleracea L.) on which the isogenic MEAM1 colony was reared. Tissues were surface sterilized by submersion in a petri dish containing 70% ethanol. The eggs were gently separated from nymphs and pupae using a small paintbrush. Isolated nymph and pupa samples were rinsed with sterile water. Approximately 1500 adult whiteflies reared on broccoli (B. oleracea L. var. botrytis) at the USDA-ARS in Charleston, SC were transferred to either TYLCV-infected or uninfected tomato (Solanum lycopersicum cv. Moneymaker) cuttings and allowed to feed for 24, 48, or 72 h, respectively. For each treatment and time point, two compound leaves were collected from TYLCV-infected or uninfected plants and transferred to a flask filled with water, which was then sealed with Parafilm and placed in an insect-proof cage. Whiteflies were added to each cage and allowed to feed for 24, 48, or 72 h under controlled conditions at 28 ± 1 °C, a 14:10 (L:D) h photoperiod, and ~60% humidity. A total of 200–500 living whiteflies were collected at the end of each time point and stored at –80 °C until processing. Three biological replicates were performed for each sample. A similar experiment under the same environmental conditions was performed using adults from a MEAM1 colony maintained at the USDA-ARS in Salinas, California (CA), but these white flies were fed on ToCV-infected or uninfected tomato (cv. Moneymaker) plants.
For insecticide treatment experiments, adults of two MED populations, PyriR, which is susceptible to the insecticide Mospilan (acetamiprid), and 9-2103, which is resistant, were fed on cotton seedlings (Gossypium hirsutum L. cv. Acala) treated with the insecticide Mospilan at an LC30 dose (lethal concentration required to kill 30% of the population; 2 ppm for PyriR and 100 ppm for 9-2013) with the dipping method, as previously described [64 (link)]. Whiteflies fed on untreated cotton seedlings were used as controls. The experiments were conducted under standard rearing room conditions of 25 °C, 50% relative humidity, and a light regime of 10 h light and 14 h dark. Three to four biological replicates, each containing a pool of 200–500 adult whiteflies, were collected from each treatment. The insects were kept at –80 °C until use.
Total RNA was purified using the Ambion TRIzol Reagent (Thermo Fisher, USA) according to the manufacturer’s protocol. Strand-specific RNA-Seq libraries were constructed following the protocol described in Zhong et al. [65 (link)] and sequenced on the Illumina HiSeq 2500 system. Raw RNA-Seq reads were first processed to remove adapter and low-quality sequences using Trimmomatic [57 (link)]. Reads shorter than 40 bp after trimming were discarded. The resulting reads were then aligned to the ribosomal RNA database [66 (link)] and the three bacterial symbiont genomes using Bowtie [67 (link)], allowing up to three mismatches. The aligned reads were not used for further analysis. To assist gene prediction, the high-quality cleaned RNA-Seq reads were aligned to the assembled B. tabaci genome using TopHat [68 (link)], and the aligned reads were assembled into transcripts using Cufflinks [69 (link)]. For gene expression analysis, the RNA-Seq reads were aligned to the assembled B. tabaci genome using HISAT [70 (link)]. Raw counts for each B. tabaci predicted gene were derived from the read alignments and normalized to fragments per kilobase of exon model per million mapped fragments (FPKM). Differential expression analyses were performed using edgeR [71 (link)]. The resulting raw P values were adjusted for multiple testing using the false discovery rate (FDR) [72 ]. For each comparison, genes with FDR <0.05 and fold change no less than 1.5 were considered as differentially expressed genes.
Full text: Click here
Publication 2016

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2019
acetamiprid Biological Markers clothianidin Creatinine Diagnosis Dry Ice Enzyme Assays Enzymes Freezing High-Performance Liquid Chromatographies Hydrolysis imidacloprid Isotopes N-desmethylacetamiprid Solid Phase Extraction Spectrometry, Mass, Electrospray Ionization Technique, Dilution thiacloprid Urine

Most recents protocols related to «Acetamiprid»

Aphids were reared according to the method described by Chang et al. [35 (link)]. A single apterous viviparous parthenogenetic A. pisum female was reared on broad bean (Vicia faba L.) seedlings in an incubator with a 16:8 h light–dark cycle, 20 °C temperature, and 60% relative humidity (RH).
For acetamiprid exposure, third-instar aphid nymphs were placed on leaves previously dipped in a 6.25 μg/mL acetamiprid solution in 0.01% dimethyl sulfoxide (DMSO) for 15~20 s, as described in Li et al. [31 (link)]. Acetamiprid acts as a systemic insecticide, controlling target insects through both contact and ingestion. Surviving aphids were transferred to fresh leaves after 72 h. This procedure was replicated in three independent experiments with 10 individuals in each replication. Aphids on leaves dipped in 0.01% DMSO served as a negative control and were designated as the non-selected (SS) strain. Acetamiprid exposure continued for nine consecutive generations to establish an acetamiprid-selected strain (RS). The criterion for selecting the RS strain at the ninth generation was based on observed differences in the growth cycle compared to the SS strain, with the RS strain requiring an additional 24 h to reach the adult stage.
Toxicity bioassays were performed as per standard leaf dip toxicity bioassay, with minor modifications [36 (link)]. Briefly, serial dilutions of an acetamiprid stock in 0.01% DMSO were prepared with 0.1% Triton X-100 in water. Medium-sized broad bean leaves were dipped into acetamiprid dilutions (12.5 μg/mL, 6.25 μg/mL, 3.12 μg/mL, 1.56 μg/mL, 0.78 μg/mL) for 30 s each and then laid flat on a non-absorbent plastic to air dry for one hour. Control leaves were treated with 0.01% DMSO in 0.1% Triton X-100 alone. Thirty pea aphids were exposed to each concentration. Assay plates were incubated with a 16:8 h light–dark cycle, 20 °C temperature, and 60% RH, and mortality was recorded after three days (i.e., dead aphid failed to respond after gentle prodding). The bioassays were repeated three times.
Full text: Click here
Publication 2024
To evaluate the direct toxicity of acetamiprid, the total number of dead cockroaches was determined for each acetamiprid concentration (0.1, 0.5, and 1 nmol.g−1) applied either by injection into the haemolymph, topical application, or oral application. The mortality was scored at 1 h, 24 h, and 48 h after acetamiprid exposure. The number of dead cockroaches observed in the corresponding control condition was used to calculate the corrected mortality percentage, using Henderson Tilton’s formula [27 (link)].
Full text: Click here
Publication 2024
Acetamiprid was applied to cockroaches in three different ways at concentrations of 0.1, 0.5, and 1 nmol.g−1 [22 (link)]. For topical application, 1 µL of the final solution was applied to the thorax (dorsal surface) of the cockroach using a Hamilton syringe. As previously described, for the haemolymph treatment, 10 µL of solution was injected between the third and fourth sternites [25 (link)]. Oral application consisted of a starvation for the day before the experiment, and then each cockroach was fed with 5 µL solution (either acetamiprid or control solution) [22 (link)]. For each condition, acetamiprid was administered to the cockroaches one time the day before the experiment.
Full text: Click here
Publication 2024
All chemicals were purchased from Sigma-Aldrich (Saint Quentin, France). Acetamiprid technical grade insecticide (CAS: 135410-20-7, purity ≥ 98%) was purchased from Sigma Aldrich (France) (Figure 1A) and was dissolved in dimethylsulfoxide (DMSO) to prepare a concentrated stock solution. The stock solution was extemporaneously diluted to obtain a working solution of acetamiprid at 0.01% DMSO. The control consisted of DMSO diluted to a final concentration of 0.01% DMSO in a saline solution. The saline solution contained (in mM): NaCl 208; KCl 3.1; CaCl2 5.4; NaHCO3 2; sucrose 26; and had a pH of 7.4 [24 (link)].
Full text: Click here
Publication 2024
Acetamiprid-N-desmethyl (190604–92-3) and luteolin (CAS Number: 491–70-3) were purchased from Sigma-Aldrich, United States.
Full text: Click here
Publication 2024

Top products related to «Acetamiprid»

Sourced in Germany, United States, India
Acetamiprid is a laboratory chemical used as an analytical standard for qualitative and quantitative analysis. It is a white crystalline solid that is soluble in organic solvents. Acetamiprid is used as a reference material in the identification and quantification of this compound in various samples.
Sourced in Canada, United States, Germany, France
Clothianidin is a neonicotinoid insecticide used in various agricultural applications. It is a broad-spectrum insecticide that acts as an agonist of the nicotinic acetylcholine receptor in the central nervous system of insects. Clothianidin is commonly used as a seed treatment or soil application to protect crops from a variety of insect pests.
Sourced in United States, Germany, China, United Kingdom, India
Thiamethoxam is a neonicotinoid insecticide developed and manufactured by Syngenta, a division of Merck Group. It is used as a broad-spectrum insecticide in agriculture and horticulture. Thiamethoxam acts as a nicotinic acetylcholine receptor agonist, affecting the central nervous system of insects.
Sourced in Germany, United States, United Kingdom
Thiacloprid is a neonicotinoid insecticide developed by Bayer CropScience. It is used in agriculture to control a variety of insect pests. Thiacloprid acts as a nicotinic acetylcholine receptor agonist, disrupting the nervous system of targeted insects.
Sourced in Germany, United States, United Kingdom, Sweden, China, Sao Tome and Principe, Italy
Imidacloprid is a chemical compound used in laboratory equipment. It functions as an insecticide, targeting the nervous system of insects. The core purpose of Imidacloprid is to provide a means of pest control in controlled laboratory environments.
Sourced in Germany, United States, Italy, India, China, United Kingdom, France, Poland, Spain, Switzerland, Australia, Canada, Brazil, Sao Tome and Principe, Ireland, Belgium, Macao, Japan, Singapore, Mexico, Austria, Czechia, Bulgaria, Hungary, Egypt, Denmark, Chile, Malaysia, Israel, Croatia, Portugal, New Zealand, Romania, Norway, Sweden, Indonesia
Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.
Sourced in United States
Clothianidin-d3 is a stable isotope-labeled compound used as an analytical standard in the detection and quantification of clothianidin, a neonicotinoid insecticide, in various matrices. It serves as a reference material for analytical methods development and validation.
Sourced in Germany
Thiamethoxam is a neonicotinoid insecticide used as a seed treatment and foliar application for the control of a variety of insect pests in various crops. It functions by interfering with the nicotinic acetylcholine receptors in the insect's nervous system, leading to paralysis and death.
Sourced in Germany
Acetamiprid is a neonicotinoid insecticide used in various agricultural and horticultural applications. It functions by disrupting the nervous system of target insect pests. Acetamiprid is commonly used to control a wide range of sucking and chewing insects.
Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan
Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.

More about "Acetamiprid"

Acetamiprid is a powerful neonicotinoid insecticide widely used to protect crops from a variety of sucking and chewing insects.
This systemic compound is effective against a broad spectrum of pests, including aphids, whiteflies, leafhoppers, and some beetle species.
Closely related to other neonicotinoids like Clothianidin, Thiamethoxam, Thiacloprid, and Imidacloprid, Acetamiprid shares similarities in chemical structure and mode of action, targeting the nicotinic acetylcholine receptors in insects.
Acetonitrile is often used as a solvent in Acetamiprid research and analysis, while Clothianidin-d3 and Methanol may be employed as internal standards and extraction solvents, respectively, during sample preparation and chromatographic techniques like HPLC and GC-MS.
Leveraging advanced AI-powered platforms can greatly optimize Acetamiprid research by providing access to the best experimental protocols from literature, preprints, and patents.
These AI-driven tools enhance reproducibility and accuracy, ensuring your Acetamiprid studies deliver reliable, high-quality results.
Experience the power of AI-driven research optimization and take your Acetamiprid research to new heights.