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Acetic Acid

Acetic Acid, a versatile organic compound, is a widely studied and widely used chemical in various industrial and research applications.
It serves as a building block for numerous other chemicals and is a key component in the production of a variety of products, from pharmaceuticals to food additives.
Acetic Acid's unique properties, such as its ability to act as a solvent, a preservative, and a pH regulator, make it an indispensable tool for researchers and scientists working in diverse fields.
This MeSH term provides a comprehensive overview of the chemical structure, properties, and applications of Acetic Acid, serving as a valuable resource for those seeking to expand their knowledge or conduct research in this area.

Most cited protocols related to «Acetic Acid»

At various time points following addition of labeled DMEM, metabolites were harvested as previously described.20 (link) After drying the samples, the metabolites extracted from the infected plates were dissolved in 600 µL of HPLC-grade water, while metabolites from mock-treated plates were dissolved in 300 µL. This 2-fold difference in volume accounts for the ~2-fold increase in volume of the fibroblasts during human cytomegalovirus infection. Volumes of 10 µL of each metabolite extract were analyzed via reversephase ion-pairing chromatography coupled to a stand-alone orbitrap mass spectrometer. The mass spectrometer scan rate was set to 1 Hz and resolving power to 100 000, scanning m/z 85–1000 in the negative ion mode. All other parameters are as in Lu et al.21 (link) The LC gradient was 0 min, 0% B; 2.5 min, 0% B; 5 min, 20% B; 7.5 min, 20% B; 13 min, 55% B; 15.5 min, 95% B; 18.5 min, 95% B; 19 min, 0% B; 25 min, 0% B. Solvent A is 97:3 water–methanol with 10 mM tributylamine and 15 mM acetic acid; solvent B is methanol. The flow rate was 200 µL/min on a Synergy Hydro-RP column (100 mm × 2 mm, 2.5 µm particle size, Phenomenex, Torrance, CA).
Publication 2010
Acetic Acid Chromatography Cytomegalovirus Infections Fibroblasts High-Performance Liquid Chromatographies Homo sapiens Human Herpesvirus 5 Infection Methanol Solvents tributylamine
DNA cleavage assay was carried out based on [3 (link)] with commercially available Cas9 enzyme (NEB). PCR amplified genomic fragments for each sgRNA-1 off-target site (OT#1_F 5’-AGAGGCAAGTAAAGGTCAAGTAGG-3’, OT#1_R 5’-TCACATTGCAATGATGAGCACTTT-3’; OT#2_F 5’-CCAGCTCATGTTGAAAAGACACAT-3’, OT#2_R 5’-CCCCCACAGATGAAATGAAAAGAC-3’; OT#3_F 5’-TACCCAAAAATTGTAAGCCAGCAG-3’, OT#3_R 5’-AGATCTGATCCGGTTTCAAAGTGA-3’) were cloned into pGEM-T easy vector (Promega). The plasmids were pre-linearized with BsaI (NEB) about 2kb from the sgRNA target site. 3nM of the pre-linearized plasmids were incubated for one 1h with 30nM sgRNA-1 and 30nM Cas9 protein (NEB) and supplemented with Cas9 nuclease buffer (NEB) in a 30μl reaction volume at 37°C. Gel electrophoresis was performed on 1.5% agarose gel in 1x TAE buffer (40mM Tris, 20mM acetic acid, 1mM EDTA).
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Publication 2015
Acetic Acid Biological Assay Buffers Cloning Vectors CRISPR-Associated Protein 9 DNA Cleavage Edetic Acid Electrophoresis Genome Plasmids Promega prostaglandin M Sepharose tris-acetate-EDTA buffer Tromethamine
Chromosomes and nuclei were spread onto glass slides following a standard procedure. Cell cultures (5 × 105 cells/ml) were incubated in medium containing colcemid (50 ng/ml, 30 min; 37°C; Sigma Chemical Co., Ltd.), washed once in HBSS, once in PBS, swollen (0.075 M KCl, 15 min; 37°C), fixed with methanol/acetic acid (3:1), dropped (three drops; 2.5 × 106 cells/ml) onto washed slides, and then air dried.
To prepare extended DNA fibers (Parra and Windle, 1993 (link)), 2 μl cells resuspended in PBS (106 cells/ml) were spotted onto cleaned glass slides and lysed with 5 μl of 0.5% SDS in 200 mM Tris-HCl, pH 7.4, 50 mM EDTA (10 min, 20°C). Slides were tilted (15° to horizontal), allowing a stream of DNA to run slowly down the slide, air dried, and then fixed in methanol/acetic acid (3:1). For most purposes, cells containing halogenated DNA were diluted 30- and 100-fold with untreated HeLa cells, before spreading. This simplifies the spreads, allows isolated labeled DNA fibers to be found with relative ease, and makes possible the identification of replicons from a single labeled cell (see Fig. 4).
To estimate the extension of DNA fibers, spreads were prepared from HeLa cells infected with adenovirus serotype 2 and grown in medium supplemented with 100 μM BrdU 15–20 h after infection (Pombo et al., 1994 (link)). Abundant Br-labeled DNA molecules measured 13.9 ± 1.3 μm (mean ± SD; n = 50). As the viral genome is 36 kbp, the extension of these DNA fibers is 2.59 ± 0.24 kbp/μm.
Publication 1998
Acetic Acid Adenovirus Vaccine Bromodeoxyuridine Cell Culture Techniques Cell Nucleus Cells Chromosomes Colcemide DNA, A-Form Edetic Acid HeLa Cells Hemoglobin, Sickle Infection Methanol Replicon Tromethamine Viral Genome
Frozen leaf material (about 100 mg FW.) was ground in liquid nitrogen with the mixer mill MM400 (Retsch GmbH, Haan, Germany) in a 2 ml Eppendorf tube, and then extracted with 1 ml of extraction solvent (methanol:isopropanol, 20:80 (v/v) with 1% of glacial acetic acid) using ultra sonication (4-7°C). The labeled forms of the compounds d4-SA, d6-ABA, d5-JA, d5-IAA, d2-GA1, d2-GA4, d2-GA9, d2-GA19, d2-GA20, d2-GA24, d4-ACC, d6-2iP, d6-IPA, d5-Z and d5-ZR were added as internal standards. D5-Z and d5-ZR were used as internal standards for DHZ and DHZR, respectively. After centrifugation (10,000 rpm for 15 min at 4°C), the supernatant was collected and the pellet was re-extracted with 0.5 ml of extraction solvent and the extraction repeated three times again. Then, supernatants were combined and dried completely under a nitrogen stream and re-dissolved in 300 μl of methanol, centrifuged (10,000 rpm for 5 min) and filtered through a 0.22 μm PTFE filter (Waters, Milford, MA, USA). Samples (5 μl) were then analyzed by UPLC/ESI-MS/MS. Hormones were determined in ten independent samples for each treatment. Quantification was done by the creation of calibration curves including each of the 17 unlabeled analyte compounds (SA, ABA, JA, IAA, GA1, GA4, GA9, GA19, GA20, GA24, ACC, 2iP, IPA, Z, ZR, DHZ and DHZR). Ten standard solutions were prepared ranging from 0.05 to 1000 ng ml-1 and for each solution a constant amount of internal standard (as described above) was added. Calibration curves for each analyte were generated using Analyst™ software (Applied Biosystems, Inc., California, USA). The limit of detection (LOD, S/N = 3) and the limit of quantification (LOQ, S/N = 10) were also calculated with the aid of this software.
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Publication 2011
Acetic Acid Centrifugation compound 17 Freezing Hormones Isopropyl Alcohol Methanol Nitrogen Plant Leaves Polytetrafluoroethylene Solvents Tandem Mass Spectrometry
Two datasets corresponding to the spectral type (CID, Low, Standard, αLP) and (ETD, Low, Standard, αLP) contain 49,167 spectra each. These datasets were generated in the Komives laboratory (University of California, San Diego). The detailed experimental procedures to generate these datasets are as follows. Wild-type S. pombe cells were lysed in: 50mM Tris-HCl pH: 8.0; 150mM NaCl; 5mM EDTA; 10% Glycerol; 50mM NaF; 0.1mM Na3VO4; 0.2% NP40 and stored at 80°C. The debris was pelleted and then the supernatant was collected. The pellet was extracted according to [56 (link)]. Briefly, the pellet was resuspended in 200 ul of 0.1 M NaOH, 0.05 M ETDA, 2% SDS, and 2% beta-mercaptoethanol and incubated at 90°C for 10 minutes. Acetic acid was added to 0.1M and vortexed followed by an additional incubation at 90°C for 10 minutes before clarification by centrifugation and Methanol/chloroform extraction. The pellet was resuspended in 100 mM Tris containing 0.1% sodium deoxycholate with TCEP at 5 mM. Free thiols were capped with n-ethylmaleimide. Excess reagent was removed by ultrafiltration with amicon-4 10 kDa centrifugal devices. The protein was then quantified and exchanged into 6M guanidine for digestion overnight by αLP. The digests were quenched by the addition of formic acid to 1%, followed by desalting by sep-pak (Waters, Milford, MA). Peptides were then fractionated with Electrostatic Repulsion-Hydrophilic Interaction Chromatography [57 (link)]. Fractions were assayed for protein concentration using a BCA assay and pooled into 18 fractions of equal protein concentration, evaporated to dryness and resuspended in 100 uL of 0.2% FA. Nano liquid chromatography tandem mass spectrometry (nLC-MS/MS) was performed with a LTQ XL mass spectrometer equipped with ETD. 10 ul of each fraction ( 1 ug) was injected onto a 12 cm × 75 um I.D.C18 column prepared in house and eluted in 0.2% FA with a gradient of 5% to 40% ACN over 60 min followed by wash and re-equilibration totaling 90 minutes of MS data per run. The flow was split about 1:500 to a flow rate of about 250 nL/min. A survey scan was followed by data dependent fragmentation of the 4 most abundant ions with both CID and ETD with supplemental activation. The maximum MS/MS ion accumulation time was set to 100 ms. Fragmented precursors were dynamically excluded for 45 seconds with one repeat allowed.
Publication 2014
2-Mercaptoethanol Acetic Acid Biological Assay Cells Centrifugation Chloroform Chromatography Deoxycholic Acid, Monosodium Salt Digestion Disgust Edetic Acid Electrostatics Ethylmaleimide formic acid Glycerin Guanidine Hydrophilic Interactions Liquid Chromatography M-200 Medical Devices Methanol Neoplasm Metastasis Peptides Proteins Radionuclide Imaging Sodium Chloride Sulfhydryl Compounds Tandem Mass Spectrometry tris(2-carboxyethyl)phosphine Tromethamine Ultrafiltration

Most recents protocols related to «Acetic Acid»

Example 30

[Figure (not displayed)]

To a stirred solution of 3-(3,4-dimethoxyphenyl)-5-(4-piperidyl)-1,2,4-oxadiazole (150 mg, 518 μmol) in N,N-dimethylformamide (1.50 mL) were added (2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate) (196 mg, 518 μmol), N-ethyl-N-(propan-2-yl)propan-2-amine (201 mg, 1.56 mmol, 271 μL), and 2-(benzylamino)acetic acid (89 mg, 544 μmol). The mixture was stirred at 20° C. for 16 h and filtered, and the crude filtrate was purified directly by prep-HPLC (column: Luna C8 100×30 5 μm; mobile phase: [water (10 mM ammonium carbonate)-acetonitrile]; B%: 30%-60%, 12 min) to give 2-(benzylamino)-1-[4-[3-(3,4-dimethoxyphenyl)-1,2,4-oxadiazol-5-yl]-1-piperidyl]ethanone (48 mg, 110 μmol, 21%) as a yellow solid. 1H NMR (400 MHz, METHANOL-d4) δ=7.65 (dd, J=1.8, 8.2 Hz, 1H), 7.57 (d, J=1.8 Hz, 1H), 7.40-7.30 (m, 4H), 7.28-7.22 (m, 1H), 7.06 (d, J=8.4 Hz, 1H), 4.45 (br d, J=13.7 Hz, 1H), 3.94-3.83 (m, 7H), 3.78 (s, 2H), 3.57-3.44 (m, 2H), 3.40-3.33 (m, 1H), 3.27-3.20 (m, 1H), 3.01 (t, J=11.2 Hz, 1H), 2.17 (dd, J=2.8, 13.3 Hz, 2H), 1.93-1.73 (m, 2H); LCMS (ESI) m/z: [M+H]+=437.3.

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Patent 2024
1H NMR Acetic Acid acetonitrile Amines ammonium carbonate Dimethylformamide High-Performance Liquid Chromatographies Lincomycin Methanol Oxadiazoles
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Example 26

[Figure (not displayed)]

Synthesis of 169-A.

A mixture of tert-butyl hexahydropyrrolo[3,4-c]pyrrole-2(1H)-carboxylate (750 mg, 3.54 mmol), 1-methylpiperidin-4-one (800 mg, 7.08 mmol) and acetic acid (2 drops) in DCE (15 mL) was stirred at 50° C. for 2 h. Then Sodium triacetoxyborohydride (1.50 g, 7.08 mmol) was added into above mixture and stirred at 50° C. for another 2 h. After the reaction was completed according to LCMS, the solvent was diluted with water (10 mL) and then extracted by DCM (10 mL×3). The combined organics washed with brine (10 mL×3), dried over anhydrous Na2SO4 and then concentrated in vacuo. The residue was purified by column chromatography on silica gel (DCM:MeOH=100:1˜50:1) to give 169-A (750 mg, 69%) as a yellow oil.

Synthesis of 169-B.

A solution of 169-A (400 mg, 1.29 mmol) in DCM (10 mL) was added TFA (5 mL) and stirred at room temperature for 1 h. when LCMS showed the reaction was finished. The solvent was removed in vacuo to give 169-B as a crude product and used to next step directly.

Synthesis of 169-C.

A mixture of 143-C (306 mg, 0.65 mmol) and 169-B (crude product from last step) in acetonitrile (6 mL) was stirred at 50° C. for 30 min. Then Na2CO3 (624 mg, 6.50 mmol) was added into above mixture and stirred at 50° C. for 3 h. After the reaction was completed according to LCMS, the mixture was cooled to room temperature. The Na2CO3 was removed by filtered. The filtrate was concentrated in vacuo. The residue was purified by column chromatography on silica gel (DCM:MeOH=100:1˜20:1) to give 169-C (230 mg, 76%) as a yellow solid.

Synthesis of 169.

A mixture of 169-C (230 mg, 0.49 mmol) and Pd/C (230 mg) in MeOH (10 mL) was stirred at room temperature for 30 min under H2 atmosphere. Pd/C was then removed by filtration through the Celite. The filtrate was concentrated and the residue was purified by Pre-TLC (DCM:MeOH=10:1) to give 169 (150 mg, 70%) as a white solid.

Compounds 152, 182, 199, 201, 202, 203, 235, 236 and 256 were synthesized in a similar manner using the appropriately substituted aldehyde or ketone variant of 169.

Compound 152.

50 mg, 36%, a light yellow solid.

Compound 182.

70 mg, 38%, a red solid.

Compound 199.

50 mg, 54%, a light yellow solid.

Compound 201.

30 mg, 42%, as a yellow solid.

Compound 202.

30 mg, 42%, a yellow solid.

Compound 203.

30 mg, 18%, a yellow solid.

Compound 235.

170 mg, 87%, a white solid.

Compound 236.

70 mg, 50%, a white solid.

Compound 256.

20 mg, 8%, a light yellow solid.

Compounds 210, 211, 215, 222, 223, 242 and 262 were synthesized in a similar manner using the appropriately substituted amine variant of 169.

Compound 210.

160 mg, 96%, a tan solid.

Compound 211.

70 mg, 40%, a white solid

Compound 215.

70 mg, 75%, a white solid.

Compound 222.

30 mg, 42%, a yellow solid.

Compound 223.

35 mg, 31%, a white solid.

Compound 242.

50 mg, 34%, a white solid.

Compound 262.

38 mg, 43%, a white solid.

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Patent 2024
Acetic Acid acetonitrile Aldehydes Amines Anabolism Atmosphere brine Celite Chromatography compound 26 compound 235 Filtration Ketones Light Lincomycin Pyrrole Silica Gel Sodium Solvents TERT protein, human

Example 12

There has been a growing interest in the fabrication of nanofibers derived from natural polymers due to their ability to mimic the structure and function of extracellular matrix. Electrospinning is a simple technique to obtain nano-micro fibers with customized fiber topology and composition (FIGS. 33A and 33B). The chitosan electrospun nanofibers have recently been extensively studied due to the favorable properties of chitosan such as controllable biodegradation, good biocompatibility and high mechanical strength. Currently, chitosan can be electrospun from a solution of chitosan dissolved in either trifluoroacetic acid (TFA) or acetic acid (HAc). However, processes to remove residual acid and acid salts from the electrospun material generally resulted in a swelling of fibers and deterioration of the nano-fibrous structure. Crosslinking in combination with neutralization methods also had not been effective at preventing loss of nano-fibrous structure.

The current study aimed to improve and maintain nano-fibrous and porous structure of the electrospun membranes by introducing a new post electrospinning chemical treatment. Membrane thickness was tripled in this research in order to increase the general tearing strength. Scanning electron micrograph (SEM) examination (FIG. 33C) and transmission electron micrograph (TEM) examination (FIG. 33D) showed Fiber diameters of the triethanolamine/N-tert-butoxycarbonyl (TEA/t-BoC) treated membranes ranged from 40 nm to 130 nm while fiber diameters were not able to be determined for the Na2CO3 group. Membranes treated by TEA/tboc (FIG. 34A) exhibited more nano-scale fibrous structure than membranes treated by saturated Na2CO3 (FIGS. 35B-35D, as seen demonstrated in scanning electron micrographs. After immersion in PBS for 24 hours, membranes treated by TEA/tboc exhibited less than 30% swelling (FIG. 34B) and retained their nanofibrous structure, compared with membranes treated by Na2CO3 (FIGS. 35B-35D) or compared with the non-treated chitosan membrane (FIG. 35A). After soaking the TEA/tBoc treated membranes in water overnight, membranes still kept the porous structure. In both, the before and after water status, fibers kept diameters in the nanometer range (FIG. 35C). TEA/tBoC modified nanofiber membranes also well preserved their fibrous structure over 4 weeks in physiological solution compared with Na2CO3 treated membranes (FIG. 35D).

Chitosan membranes treated by TEA/tboc showed better nano-fiber morphology characteristics than membranes neutralized by saturated Na2CO3 solution before and after being soaked in PBS. Retention of the nanofibrous structure for guided tissue regeneration applications may be of benefit for enabling nutrient exchange between soft gingival tissue and bone compartments and for mimicking the natural nanofibrillar components of the extracellular matrix during regeneration.

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Patent 2024
Acetic Acid Acids Bones Chitosan Electrons Environmental Biodegradation Extracellular Matrix Fibrosis Gingiva Guided Tissue Regeneration Hydrochloric acid Nutrients physiology Polymers Regeneration Retention (Psychology) Submersion TERT protein, human Tissue, Membrane Tissues Transmission, Communicable Disease triethanolamine Trifluoroacetic Acid Vision
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Example 125

[Figure (not displayed)]

Methyl 4-((5-(benzyloxy)-2-methoxyphenyl)(ethyl)amino)butanoate (184). 5-(Benzyloxy)-N-ethyl-2-methoxyaniline (146) (0.681 g, 2.65 mmol), DIEA (0.92 mL, 5.3 mmol), and methyl 4-iodobutyrate (0.72 mL, 5.3 mmol) in DMF (5 mL) were stirred at 70° C. for 5 days. The reaction mixture was cooled to rt, diluted with EtOAc (60 mL), washed with water (4×50 mL), brine (75 mL), dried over Na2SO4 and evaporated. The residue was purified by chromatography on a silica gel column (2.5×30 cm bed, packed with CHCl3), eluant: 5% MeOH in CHCl3 to get compound 184 (0.72 g, 76%) as a dark amber oil.

Methyl 4-(ethyl(5-hydroxy-2-methoxyphenyl)amino)butanoate (186). Ester 184 (0.72 g, 2.0 mmol) was stirred under reflux with 6 mL of water and 6 mL of conc HCl for 1.5 hrs and then evaporated to dryness to give acid 185 as a brown gum. The crude acid was dissolved in 50 mL of methanol containing 1 drop (cat.) of methanesulfonic acid ant the solution was kept for 2 hrs at rt. After that the mixture was concentrated in vacuum and the residue was mixed with 20 mL of saturated NaHCO3. The product was extracted with EtOAc (3×40 mL). The extract was washed with brine (40 mL), dried over Na2SO4 and evaporated. The residue was purified by chromatography on a silica gel column (2.5×30 cm bed, packed with CHCl3), eluant: 5% MeOH in CHCl3 to get compound 186 (0.444 g, 83%) as a brown oil.

N-(6-(dimethylamino)-9-(4-(ethyl(4-methoxy-4-oxobutyl)amino)-2-hydroxy-5-methoxyphenyl)-3H-xanthen-3-ylidene)-N-methylmethanaminium chloride (187). To a stirred suspension of tetramethylrhodamine ketone 101 (0.234 g, 0.830 mmol) in 10 mL of dry chloroform was added oxalyl chloride (72 μL, 0.82 mmol) upon cooling to 0-5° C. The resulting red solution was stirred for 0.5 h at 5° C., and the solution of compound 186 (0.222 g, 0.831 mmol) in dry chloroform (5 mL) was introduced. The reaction was allowed to heat to rt, stirred for 72 h, diluted with CHCl3 (100 mL and washed with sat. NaHCO3 solution (2×30 mL) The organic layer was extracted with 5% HCl (3×25 mL). The combined acid extract was washed with CHCl3 (2×15 mL; discarded), saturated with sodium acetate and extracted with CHCl3 (5×30 mL). The extract was washed with brine (50 mL), dried over Na2SO4 and evaporated. The crude product was purified by chromatography on silica gel column (2×50 cm bed, packed with CHCl3/MeOH/AcOH/H2O (100:20:5:1)), eluant: CHCl3/MeOH/AcOH/H2O (100:20:5:1) to give the product 187 (0.138 g, 29%) as a purple solid.

4-((4-(6-(dimethylamino)-3-(dimethyliminio)-3H-xanthen-9-yl)-5-hydroxy-2-methoxyphenyl)(ethyl)amino)butanoate (188). Methyl ester 187 (0.136 g, 0.240 mmol) was dissolved in 5 mL of 1M KOH (5 mmol). The reaction mixture was kept at rt for 1.5 hrs and the acetic acid (1 mL) was added. The mixture was extracted with CHCl3 (4×30 mL), and combined extract was washed with brine (20 mL), filtered through the paper filter and. The crude product was purified by chromatography on silica gel column (2×50 cm bed, packed with MeCN/H2O (4:1)), eluant: MeCN/H2O/AcOH/(4:1:1) to give the product 188 (0.069 g, 98%) as a purple solid.

N-(6-(dimethylamino)-9-(4-((4-(2,5-dioxopyrrolidin-1-yloxy)-4-oxobutyl)(ethyl)amino)-2-hydroxy-5-methoxyphenyl)-3H-xanthen-3-ylidene)-N-methylmethanaminium chloride (189). To a solution of the acid 188 (69 mg, 0.12 mmol) in DMF (2 mL) and DIEA (58 μL, 0.33 mmol) was added N-hydroxysuccinimide trifluoroacetate (70 mg, 0.33 mmol). The reaction mixture was stirred for 30 min, diluted with chloroform (100 mL) and washed with water (5×50 mL), brine (50 mL), filtered through paper and concentrated in vacuum. The crude product was purified by precipitation from CHCl3 solution (5 mL) with ether (20 mL) to give compound 189 (55 mg, 67%) as a purple powder.

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Patent 2024
Acetic Acid Acids Amber Anabolism Bicarbonate, Sodium brine Chlorides Chloroform Chromatography Esters Ethyl Ether Hydroxyl Radical Ketones methanesulfonic acid Methanol N,N-diisopropylethylamine N-hydroxysuccinimide oxalyl chloride Powder Silica Gel Sodium Acetate tetramethylrhodamine Trifluoroacetate Vacuum

Example 22

[Figure (not displayed)]

To a stirred solution of 3-(3,4-dimethoxyphenyl)-5-(4-piperidyl)-1,2,4-oxadiazole (150 mg, 518 μmol) in N,N-dimethylformamide (2 mL) was added (2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate) (196 mg, 518 μmol) and N-ethyl-N-(propan-2-yl)propan-2-amine (201 mg, 1.56 mmol, 271 μL) and 2-[benzoyl(methyl)amino]acetic acid (105 mg, 544 μmol). The mixture was stirred at 20° C. for 5 h, then cooled and purified directly by prep-HPLC (column: Luna C8 100×30 5 μm; mobile phase: [water (10 mM ammonium carbonate)-acetonitrile]; B%: 30%-60%, 12 min) to give N-[2-[4-[3-(3,4-dimethoxyphenyl) -1,2,4-oxadiazol-5-yl]-1-piperidyl]-2-oxo-ethyl]-N-methyl-benzamide (133 mg, 282 μmol, 54%) as a white solid. 1H NMR (400 MHz, DMSO-d6) δ=7.59 (dd, J=1.8, 8.4 Hz, 1H), 7.49-7.32 (m, 5H), 7.27 (br d, J=6.8 Hz, 1H), 7.16-7.08 (m, 1H), 4.44-4.24 (m, 2H), 4.21-4.03 (m, 1H), 4.02-3.88 (m, 1H), 3.88-3.74 (m, 6H), 3.56 (br d, J=13.7 Hz, 1H), 3.48-3.33 (m, 1H), 3.11-2.77 (m, 5H), 2.20-1.99 (m, 2H), 1.86 (br t, J=12.6 Hz, 1H), 1.74-1.48 (m, 2H), 1.43-1.26 (m, 1H); LCMS (ESI) m/z: [M+H]+=465.3.

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Patent 2024
1H NMR Acetic Acid acetonitrile Amines ammonium carbonate benzamide Dimethylformamide High-Performance Liquid Chromatographies Lincomycin N-methylbenzamide Oxadiazoles Sulfoxide, Dimethyl

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Acetic acid is a colorless, vinegar-like liquid chemical compound. It is a commonly used laboratory reagent with the molecular formula CH3COOH. Acetic acid serves as a solvent, a pH adjuster, and a reactant in various chemical processes.
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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
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Glacial acetic acid is a colorless, odorous, and corrosive liquid used as a laboratory reagent. It has a chemical formula of CH3COOH and a concentration of 99.7% or higher. Glacial acetic acid is commonly used in various analytical and research applications, serving as a solvent, catalyst, and pH modifier.
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Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.
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Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.
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Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.
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Chitosan is a natural biopolymer derived from the exoskeletons of crustaceans, such as shrimp and crabs. It is a versatile material with various applications in the field of laboratory equipment. Chitosan exhibits unique properties, including biocompatibility, biodegradability, and antimicrobial activity. It can be utilized in the development of a wide range of lab equipment, such as filters, membranes, and sorbents, due to its ability to interact with various substances and its potential for customization.
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Formic acid is a colorless, pungent-smelling liquid chemical compound. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid is widely used in various industrial and laboratory applications.
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Gallic acid is a naturally occurring organic compound that can be used as a laboratory reagent. It is a white to light tan crystalline solid with the chemical formula C6H2(OH)3COOH. Gallic acid is commonly used in various analytical and research applications.
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Ethanol is a clear, colorless liquid chemical compound commonly used in laboratory settings. It is a key component in various scientific applications, serving as a solvent, disinfectant, and fuel source. Ethanol has a molecular formula of C2H6O and a range of industrial and research uses.

More about "Acetic Acid"

Acetic acid, also known as ethanoic acid, is a widely studied and versatile organic compound with numerous industrial and research applications.
It serves as a fundamental building block for a vast array of chemicals, including pharmaceuticals, food additives, and other essential products.
One of the key properties of acetic acid is its ability to act as a solvent, preservative, and pH regulator, making it an indispensable tool for researchers and scientists across diverse fields.
Acetic acid's unique chemical structure and properties allow it to be used in a variety of applications, from chemical synthesis to food preservation.
Closely related to acetic acid are other important compounds like methanol, glacial acetic acid, sodium hydroxide, acetonitrile, hydrochloric acid, chitosan, formic acid, gallic acid, and ethanol.
These substances often play complementary roles in research, industrial processes, and product formulations involving acetic acid.
The comprehensive understanding of acetic acid's structure, properties, and applications, as provided by the MeSH term description, serves as a valuable resource for those seeking to expand their knowledge or conduct research in this area.
Leveraging cutting-edge technologies like PubCompare.ai's AI-driven platform can further optimize and streamline the research process, helping researchers identify the most effective protocols and solutions for their acetic acid-related projects.