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AG-1478

AG-1478 is a tyrosine kinase inhibitor that targets the epidermal growth factor receptor (EGFR).
It has been studied for its potential therapeutic applications in various disease models, including cancer.
PubCompare.ai is a powerful platform that helps researchers optimize their AG-1478 research protocols for reproducibility and accuracy.
The platform utilizes AI-driven comparisons to locate the best protocols from literature, preprints, and patents, enabling researchers to improve their AG-1478 studies and avoid common tyops that can impact their findings.

Most cited protocols related to «AG-1478»

Esophageal epithelial cell culture and TGF-β1 treatment

Cited 10 times

EPC1-hTERT and EPC2-hTERT, established from independent primary cultures of normal human esophageal epithelial cells, and their derivatives were grown in Keratinocyte-serum free medium (Invitrogen, Carlsbad, CA) at 37°C in a 5% CO₂ atmosphere as described previously (18 (link), 20 (link), 22 (link)–23 (link)). HCE7, an ESCC cell line was grown as described previously (24 (link)). Countess^™ Automated Cell Counter (Invitrogen) was used to count cells with 0.2% Trypan Blue dye to exclude dead cells. Cells were treated with 5 ng/ml of recombinant human TGF-β1 (R&D Systems, Minneapolis, MN) reconstituted in 4 mM HCl containing 0.1% bovine serum albumin. AG 1478 (Calbiochem, La Jolla CA) was reconstituted in dimethyl sulfoxide and used at 100 nM. Phase contrast images were acquired using a Nikon Eclipse TS100 microscope. Spindle-shaped cells were scored by counting at least 100 cells per high-power field (n=6) under light microscopy.

Ohashi S., Natsuizaka M., Wong G.S., Michaylira C.Z., Grugan K.D., Stairs D.B., Kalabis J., Vega M.E., Kalman R.A., Nakagawa M., Klein-Szanto A.J., Herlyn M., Diehl J.A., Rustgi A.K, & Nakagawa H. (2010). EGFR and mutant p53 expand esophageal cellular subpopulation capable of epithelial-to-mesenchymal transition through ZEB transcription factors. Cancer research, 70(10), 4174-4184.

Publication 2010

2-(2-(2-chloro-3-(2-(3,3-dimethyl-5-sulfo-1-(4-sulfo-butyl)-3H-indol-2-yl)-vinyl)-cyclohex-2-enylidene)-ethylidene)-3,3-dimethyl-1-(4-sulfo-butyl)-2,3-dihydro-1H-indole-5-carboxylic acid AG-1478 Atmosphere Cell Culture Techniques Cell Lines Cells derivatives Homo sapiens Keratinocyte Light Microscopy Microscopy Microscopy, Phase-Contrast Primary Cell Culture Serum Serum Albumin, Bovine Sulfoxide, Dimethyl TGF-beta1 Trypan Blue

Regulation of EGFR Signaling by miR-203

Cited 5 times

EGF was from R&D (R&D Systems, MN), and EGFR inhibitor (CI1033 and AG1478) was from Selleck (Selleck, TX). The BD Matrigel™ was purchased from BD Biosciences (BD Biosciences, CA) for invasion assay and tumor cell injections. Precursor for miRNAs (empty vector and miR-203 precursor) and anti-miR inhibitors (control and anti-miR-203) were from GeneCopoeia (GeneCopoeia, MD). RFP reporter vectors were constructed using the Clone-it Enzyme free Lenti-vectors Kit (System Biosciences, CA). Human AREG, EREG, and TGFA full length 3'UTR reporters were constructed using the psiHECKTM-2 vector (Promega, WI). The microRNA binding site mutations were made using the Site-Directed Mutagenesis System kit (Invitrogen, CA). All primers used for these constructs are listed in Supplemental Tables (Table S1). All constructs were verified by DNA sequence analysis.

Siu M.K., Abou-Kheir W., Yin J.J., Chang Y.S., Barrett B., Suau F., Casey O., Chen W.Y., Fang L., Hynes P., Hsieh Y.Y., Liu Y.N., Huang J, & Kelly K. (2014). Loss of EGFR signaling-regulated miR-203 promotes prostate cancer bone metastasis and tyrosine kinase inhibitors resistance. Oncotarget, 5(11), 3770-3784.

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Publication 2014

3' Untranslated Regions AG-1478 Antagomirs AREG protein, human Binding Sites Biological Assay Cells CI1033 Clone Cells Cloning Vectors EGFR protein, human Enzymes EREG protein, human Homo sapiens inhibitors matrigel MicroRNAs Mutagenesis, Site-Directed Mutation Neoplasms Oligonucleotide Primers Promega Sequence Analysis, DNA TGFA protein, human

Mucin Production in SP-A Knockout Mice

Cited 4 times

Mice were maintained in pathogen-free housing at Duke University. SP-A^−/− mice were obtained (25 (link)) and crossed onto the C57BL/6 background after which they were bred in-house. Wild-type (WT) mice were purchased from Jackson Laboratories (Bar Harbor, ME). Sex-matched female mice were used for experiments and were approximately 6-8 weeks of age. Mice were anesthetized by isoflourane inhalation, and given 5 μg of MMF in 50 μl of sterile saline via oropharyngeal delivery. At the desired time point, mice were euthanized and lavaged with PBS (0.1 mM EDTA) and lung tissue was obtained for further analysis. For analysis of mucin production, mice were harvested either at 16 or 24 hrs post MMF exposure as noted in the text for each experiment; for p-EGFR staining, mice were harvested 12 hrs after MMF exposure. Some mice were given an EGFR inhibitor, AG1478 (Selleckchem), via ip injection 30 minutes prior to receiving oropharyngeal delivery of MMF. AG1478 was solubilized in DMSO and diluted to a concentration of 10 mg/kg mouse weight in sterile saline. The vehicle control was DMSO diluted in sterile saline.

Ledford J.G., Voelker D.R., Addison K.J., Wang Y., Nikam V., Degan S., Kandasamy P., Tanyaratsrisakul S., Fischer B.M., Kraft M, & Hollingsworth J.W. (2015). Genetic variation in Surfactant Protein-A2 (SP-A2) leads to differential binding to Mycoplasma pneumoniae membranes and regulation of host responses. Journal of immunology (Baltimore, Md. : 1950), 194(12), 6123-6132.

Publication 2015

AG-1478 Edetic Acid EGFR protein, human Females Inhalation Injections, Intraperitoneal Lung Mice, House Mucins Obstetric Delivery Oropharynxs Pathogenicity Saline Solution Sterility, Reproductive Sulfoxide, Dimethyl Tissues

Quantifying HSV-1 Viral Entry and Infection

Cited 4 times

Viral entry was detected as described previously (7 (link), 33 (link), 73 (link)). Briefly, cells were seeded 24 h prior to experimentation in 12-well or 24-well plates. The cells were pretreated with inhibitors at 37°C for different times, and then HSV-1 (multiplicity of infection [MOI] = 20) binding to cells was performed for 1 h at 4°C in the absence of inhibitors. Then, the cells were washed with PBS (pH 3.0) to remove the bound but not entered virions and incubated at 37°C with the indicated compounds for 1 h. The cells were then washed three times with PBS and digested and recovered by centrifugation, and the internalized viral DNA was isolated using a UNIQ-10 viral DNA kit (Sangon; China). HSV-1 infection and entry were assessed by detecting the viral UL47 gene and UL46 gene using real-time DNA PCR and were expressed relative to control infections without the addition of inhibitors. The PCR ampliﬁcation product of UL46/UL47 was puriﬁed using Gel Extraction Kit II (U-gene; China), diluted serially, and used as a standard for quantitative analysis. The initial copy number of UL46/UL47 DNA in each group was calculated using the following formula: C_T = −K logX0 + b, where C_T is the cycle threshold and K, X0, and b refer to the slope rate, initial copy number, and constant, respectively. For the HSV-1 binding assay, cells were incubated with virus in the presence of inhibitors at 4°C for 1 h, and then cells were washed to remove unbounded virus. Total viral DNA was extracted and measured by real-time PCR. To study the EGFR effect on HSV-1 entry, AG-1478 inhibitor was added every 20 min after viral binding. To assess the effect of EGF on HSV-1 entry, serum-starved SK–N–SH cells were preincubated in serum-free medium in the presence or absence of EGF prior to HSV-1 binding and entry in the presence or absence of EGF. For the mRNA expression level assay, total RNA was extracted with TRIzol reagent (Invitrogen), and 1 µg of RNA was then reverse transcribed with a PrimeScript RT reagent kit (TaKaRa).

Zheng K., Xiang Y., Wang X., Wang Q., Zhong M., Wang S., Wang X., Fan J., Kitazato K, & Wang Y. (2014). Epidermal Growth Factor Receptor-PI3K Signaling Controls Cofilin Activity To Facilitate Herpes Simplex Virus 1 Entry into Neuronal Cells. mBio, 5(1), e00958-13.

Publication 2014

AG-1478 Biological Assay Cells Centrifugation DNA, Viral EGFR protein, human Genes Genes, Viral Herpes Simplex Human Herpesvirus 1 Infection inhibitors Real-Time Polymerase Chain Reaction RNA, Messenger Serum trizol Virion Virus Virus Internalization

Accelerated Anti-GBM RPGN Model Experiments

Cited 4 times

We used the accelerated anti-GBM RPGN model, as previously described^{11 (link),30 (link)}. After nephrotoxic serum (NTS) injection, mice were given repeated intraperitoneal injections of either AG1478 (LC Laboratories) (30 mg.kg⁻¹.day⁻), or vehicle (n=7 per group). On day 8, we collected urine and euthanized mice. In another set of experiments, three groups of mice (n=9 per group) received erlotinib (10 mg.kg⁻¹.day⁻¹ per gavage), started erlotinib either on day 0 or day 4, or vehicle, until sacrifice on day 14. We repeated the experiment for electron microscopy study, mice receiving erlotinib or vehicle (n=3 per group), from day 0 to sacrifice on day 5.

Bollée G., Flamant M., Schordan S., Fligny C., Rumpel E., Milon M., Schordan E., Sabaa N., Vandermeersch S., Galaup A., Rodenas A., Casal I., Sunnarborg S.W., Salant D.J., Kopp J.B., Threadgill D.W., Quaggin S.E., Dussaule J.C., Germain S., Mesnard L., Endlich K., Boucheix C., Belenfant X., Callard P., Endlich N, & Tharaux P.L. (2011). The Epidermal Growth Factor Receptor Promotes Glomerular Injury and Renal Failure in Rapidly Progressive Crescentic Glomerulonephritis; the Identification of Possible Therapy. Nature medicine, 17(10), 1242-1250.

Publication 2011

AG-1478 antiglomerular basement membrane antibody Electron Microscopy Erlotinib Injections, Intraperitoneal Mice, House Serum Tube Feeding Urine

Most recents protocols related to «AG-1478»

Silencing ARAP1 and ARAP1-AS2 in HRMCs

We used the same methods as in our previous reports for synthesizing shRNA targeting human ARAP1 and siRNA targeting human ARAP1-AS2 (Li et al., 2020a (link); Li et al., 2020b (link)). The siRNAs targeting mouse ARAP1, overexpression plasmids and siRNAs targeting human YY1 and overexpression plasmids targeting human ARAP1were purchased from Sangon Biotechnology (Shanghai, China). The specific EGFR tyrosine kinase inhibitor AG1478 (MedChemExpress LLC) was used to suppress the phosphorylation of EGFR in HRMCs. Powdered AG1478 was dissolved in DMSO, and the final concentration of AG1478 administered to HRMCs was 10 μmol/L. Cells were exposed to AG1478 for 48 h. Transfection methods, cell lines created by transfection and their names are described in the Supplementary Material and Methods.

Li X., Ma T.K., Wang M., Zhang X.D., Liu T.Y., Liu Y., Huang Z.H., Zhu Y.H., Zhang S., Yin L., Xu Y.Y., Ding H., Liu C., Shi H, & Fan Q.L. (2023). YY1-induced upregulation of LncRNA-ARAP1-AS2 and ARAP1 promotes diabetic kidney fibrosis via aberrant glycolysis associated with EGFR/PKM2/HIF-1α pathway. Frontiers in Pharmacology, 14, 1069348.

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Publication 2023

AG-1478 Cell Lines Cells EGFR protein, human Homo sapiens Mus Phosphorylation Plasmids RNA, Small Interfering Short Hairpin RNA Sulfoxide, Dimethyl Transfection

Zebrafish Pharmacological Assays

MTZ (MilliporeSigma, M3761-25G) was dissolved in system water at a concentration of 5 mM and 0.5% DMSO. Adult zebrafish were exposed to MTZ for 16 hours at ambient temperature in the dark. APAP (MilliporeSigma, A7085-100G) was dissolved in system water at a concentration of 15 mM. EtOH (MilliporeSigma, EX0276-3) was dissolved in system water at a concentration of 1%. Adult zebrafish were exposed to APAP or EtOH for 24 hours at ambient temperature in the dark. Stock solutions for AG1478 (Selleck Chemicals, S2728), U0126 (Cayman Chemical Company, 70970), LY290002 (Selleck Chemicals, S1105), and Rapamycin (Selleck Chemicals, AY-22989) were made by dissolution in DMSO at a concentration of 10 μM, 20 μM, 10 μM, and 10 μM, respectively. These chemicals were diluted 1:1,000 in system water to make a working solution. Adult zebrafish were immersed in the working solution. Working solution was changed daily. 4-OHT (Hello Bio, HB2508) was dissolved in DMSO at a concentration of 10 mM, vortexed continuously for 15 minutes, and stored at –20°C. Prior to use, an aliquot of 4-OHT was incubated at 65°C for 10 minutes. Triple-transgenic larvae were exposed to 4-OHT between 2 and 3 days after fertilization to induce recombination.

Oderberg I.M, & Goessling W. (2023). Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish. JCI Insight, 8(1), e163929.

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Publication 2023

4,17 beta-dihydroxy-4-androstene-3-one Acetaminophen Adult AG-1478 Animals, Transgenic AY 22-989 Caimans Ethanol Fertilization Larva Recombination, Genetic Sirolimus Sulfoxide, Dimethyl U 0126 Zebrafish

Signaling Pathways Regulation in Cellular Processes

Dulbecco’s modified Eagle’s medium (DMEM)/F-12 medium, fetal bovine serum (FBS), and TRIzol reagent were procured from Invitrogen (Carlsbad, CA, USA). Hybond C membrane and the enhanced chemiluminescence (ECL) Western blot detection system were obtained from GE Healthcare Biosciences (Buckinghamshire, England, UK). Antibodies against phosphorylated proteins, including anti-phospho-PKCδ (#9374, Thr505), anti-phospho-Pyk2 (#3291, Tyr402), anti-phospho-c-Src (#2101, Tyr416), anti-phospho-EGFR (#4407, Tyr1173), anti-phospho-Akt (#9271, Ser473), anti-phospho-c-Jun (#2361, Ser63), and anti-COX-2 (#12282), were sourced from Cell Signaling (Danvers, MA, USA). Antibodies against non-phosphorylated proteins, such as anti-PKCδ (sc-213), anti-c-Src (sc-18), anti-EGFR (sc-03-G), anti-Akt (sc-8312), and anti-c-Jun (sc-44), were obtained from Santa Cruz (Santa Cruz, CA, USA). The antibody against Pyk2 (ab55358) was procured from Abcam (Cambridge, MA, USA). The anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (mouse monoclonal antibody, Cat# MCA-1D4) was sourced from EnCor Biotechnology (Gainesville, FL, USA). Chemical compounds, including Rottlerin, PF431396, PP1, AG1478, LY294002, and Tanshinone IIA, were supplied by Biomol (Plymouth Meeting, PA, USA). Supplies for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were obtained from MDBio Inc. (Taipei, Taiwan). Thrombin (BRENDA: EC3.4.21.5, from bovine plasma, Cat# T4648), enzymes, and other chemicals were purchased from Sigma (St. Louis, MO, USA).

Yang C.C., Lee I.T., Lin Y.J., Wu W.B., Hsiao L.D, & Yang C.M. (2023). Thrombin-Induced COX-2 Expression and PGE2 Synthesis in Human Tracheal Smooth Muscle Cells: Role of PKCδ/Pyk2-Dependent AP-1 Pathway Modulation. International Journal of Molecular Sciences, 24(20), 15130.

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Publication 2023

AG-1478 Antibodies Bos taurus Chemiluminescence Eagle EGFR protein, human Enzymes Fetal Bovine Serum Glyceraldehyde-3-Phosphate Dehydrogenases Immunoglobulins LY 294002 Monoclonal Antibodies Mus Plasma Proteins PTGS2 protein, human rottlerin SDS-PAGE tanshinone II A Thrombin Tissue, Membrane trizol Western Blotting

Silica Nanoparticle-Induced Cellular Responses

SiNPs (nanopowder, particle size between 10 and 20 nm, # 637238) were purchased from Sigma (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM)/F-12 medium, fetal bovine serum (FBS), TRIZOL reagent, 2′,7′-dichlorofluorescin diacetate (H₂DCF-DA), and M-MLV Reverse Transcriptase kit were purchased from Invitrogen (Carlsbad, CA, USA). BioTrace™ NT nitrocellulose transfer membrane was from Pall Corporation (Port Washington, NY, USA). Enhanced chemiluminescence reagent was from EMD Millipore Corporation (Burlington, MA, USA). GenMute^TM siRNA Transfection Reagent was obtained from SignaGen Lab (Gaithersburg, MD, USA). Actinomycin D (Act. D), cycloheximide (CHI), edaravone, AG1478, PF-431396, LY294002, Akt inhibitor (Akti) VIII, p38 MAPK inhibitor (p38i) VIII, SP600125, AS1842856, tanshinone IIA were purchased from Biomol (Plymouth Meeting, PA, USA). Anti-COX-2 (#12282S), anti-GAPDH (#2118L), anti-phospho-Pyk2 (#3291S), anti-phospho-EGFR (Tyr¹¹⁷³, #4407L), anti-P110 (#4249S), anti-phospho-Akt (Ser⁴⁷³, #9271L), anti-Akt (#4691L), anti-phospho-p38 MAPK (Thr¹⁸⁰/Tyr¹⁸², #9211L), anti-p38 MAPK (#8690S), anti-phospho-SAPK/JNK (Thr¹⁸³/Tyr¹⁸⁵, #4668S), anti-phospho-FoxO1 (#9461S), anti-FoxO1 (#2880S), anti-phospho-c-Jun (#2361S), and anti-c-Jun (#9165L) antibodies were from Cell Signaling Technology (Danvers, MA). Anti-Pyk2 (#ab32448) antibody was from Abcam (Cambridge, UK). Anti-EGFR (sc-373746) and anti-JNK1/2 (sc-137020) antibodies were from Santa Cruz (Santa Cruz, CA, USA). Enzymes and other chemicals were from Sigma (St. Louis, MO, USA).

Lin Y.J., Yang C.C., Lee I.T., Wu W.B., Lin C.C., Hsiao L.D, & Yang C.M. (2023). Reactive Oxygen Species-Dependent Activation of EGFR/Akt/p38 Mitogen-Activated Protein Kinase and JNK1/2/FoxO1 and AP-1 Pathways in Human Pulmonary Alveolar Epithelial Cells Leads to Up-Regulation of COX-2/PGE2 Induced by Silica Nanoparticles. Biomedicines, 11(10), 2628.

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Publication 2023

1,3-dihydro-1-(1-((4-(6-phenyl-1H-imidazo(4,5-g)quinoxalin-7-yl)phenyl)methyl)-4-piperidinyl)-2H-benzimidazol-2-one AG-1478 Antibodies AS 1842856 Chemiluminescence Cycloheximide Dactinomycin dichlorofluorescin Eagle Edaravone EGFR protein, human Enzymes Fetal Bovine Serum GAPDH protein, human Immunoglobulins LY 294002 Mitogen-Activated Protein Kinase p38 Nitrocellulose PTGS2 protein, human RNA, Small Interfering RNA-Directed DNA Polymerase SP600125 tanshinone II A Tissue, Membrane Transfection trizol

Modulation of Liver Lipid Metabolism

Tg(fabp10a:pt-β-catenin) larvae were treated with 0.1% dimethyl sulfoxide (DMSO), 1 µM GW7647 (Cayman Chemical, Ann Arbor, MI), 2 µM K-975 (MedChemExpress, Princeton, NJ), 15 µM Etomoxir (Cayman Chemical, Ann Arbor, MI), 2 µM AG1478 (ApexBio, Houston, TX), or 7 μM CAY10599 (Cayman Chemical, Ann Arbor, MI) for 48 h from 12 or 13 days post-fertilization (dpf). Compounds were refreshed every 24 h. The larvae were fed prior to the drug treatments but fasted during the treatments. They were harvested at 14 or 15 dpf for subsequent analyses.

Kim M., So J, & Shin D. (2023). PPARα activation promotes liver progenitor cell-mediated liver regeneration by suppressing YAP signaling in zebrafish. Scientific Reports, 13, 18312.

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Publication 2023

AG-1478 Caimans CTNNB1 protein, human etomoxir Fertilization GW 7647 Larva Pharmaceutical Preparations Sulfoxide, Dimethyl

Top products related to «AG-1478»

Ag1478 by Merck Group

Sourced in United States, Germany, Japan, Sao Tome and Principe, Macao

AG1478 is a tyrosine kinase inhibitor. It functions by inhibiting the epidermal growth factor receptor (EGFR).

Ag1478 by Selleck Chemicals

Sourced in United States, Germany, China

AG1478 is a chemical compound that functions as a tyrosine kinase inhibitor. It is used as a research tool in cell culture and biochemical studies. The core function of AG1478 is to selectively inhibit the epidermal growth factor receptor (EGFR) tyrosine kinase activity.

Ly294002 by Merck Group

Sourced in United States, Germany, United Kingdom, China, Sao Tome and Principe, Macao, Italy, Canada, Switzerland, Japan, France, Israel, Spain, Morocco

LY294002 is a chemical compound that functions as a specific inhibitor of phosphoinositide 3-kinase (PI3K). It is commonly used in laboratory research settings to investigate the role of PI3K signaling pathways.

Fetal bovine serum (fbs) by Thermo Fisher Scientific

Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal

Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Epidermal growth factor (egf) by Merck Group

Sourced in United States, Germany, United Kingdom, China, Italy, Canada, Japan, Spain, France, Macao, Israel, Australia, Denmark, Sao Tome and Principe, Morocco, Austria, Portugal, Belgium, Poland, Switzerland

EGF is a laboratory equipment product manufactured by Merck Group. It is a protein that plays a key role in cell growth and differentiation processes. EGF primarily functions as a growth factor, stimulating cell division and proliferation in various cell types.

Ag1478 by Bio-Techne

Sourced in United Kingdom

AG1478 is a highly selective inhibitor of the epidermal growth factor receptor (EGFR) tyrosine kinase. It blocks EGFR autophosphorylation and downstream signaling pathways.

Dimethyl sulfoxide (dmso) by Merck Group

Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh

DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.

Tyrphostin ag 1478 by Merck Group

Sourced in United States, Germany

Tyrphostin AG 1478 is a small-molecule compound commonly used as a tool in laboratory research. It functions as a selective inhibitor of the epidermal growth factor receptor (EGFR) tyrosine kinase. This compound can be utilized to study the role of EGFR signaling in various cellular processes and pathways.

Ag1478 by Cayman Chemical

Sourced in United States

AG1478 is a selective inhibitor of the epidermal growth factor receptor (EGFR) tyrosine kinase. It inhibits the enzymatic activity of EGFR, which is involved in cellular signaling pathways.

Epidermal growth factor (egf) by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Germany, China, Canada, France, Italy, Japan, Israel, Switzerland, Australia, Macao, Belgium, Spain, Denmark, Jersey

EGF is a lab equipment product from Thermo Fisher Scientific. It is a recombinant human Epidermal Growth Factor (EGF) protein. EGF is a growth factor that plays a role in cell proliferation and differentiation.

What is AG-1478 and what are its potential therapeutic applications?

AG-1478 is a tyrosine kinase inhibitor that targets the epidermal growth factor receptor (EGFR). It has been studied for its potential therapeutic applications in various disease models, including cancer. AG-1478 has shown promise in preclinical studies for its ability to inhibit EGFR signaling and potentially treat conditions where EGFR plays a key role, such as certain types of cancer.

What are some common challenges researchers face when working with AG-1478?

One common challenge researchers face when working with AG-1478 is ensuring the reproducibility and accuracy of their research protocols. Small variations in experimental conditions, such as typos in reagent concentrations or incubation times, can significantly impact the results. This makes it critical to identify and implement the most effective and robust protocols for AG-1478 studies.

How can PubCompare.ai help researchers optimize their AG-1478 research protocols?

PubCompare.ai is a powerful platform that can assist researchers in optimizing their AG-1478 research protocols for reproducibility and accuracy. The platform utilizes AI-driven comparisons to locate the best protocols from literature, preprints, and patents, enabling researchers to improve their AG-1478 studies and avoid common tyops that can impact their findings. PubCompare.ai allows researchers to screen protocol literature more efficiently, leverage AI to pinpoint critical insights, and identify the most effective protocols related to AG-1478 for their specific research goals.

What are the key benefits of using PubCompare.ai for AG-1478 research?

The key benefits of using PubCompare.ai for AG-1478 research include improved reproducibility, increased accuracy, and more efficient protocol selection. The platform's AI-driven analysis can highlight key differences in protocol effectiveness, enabling researchers to choose the best option for their specific research goals. This helps researchers avoid common pitfalls, such as typos in reagent concentrations, that can negatively impact their findings and the overall quality of their AG-1478 studies.

More about "AG-1478"

AG-1478, also known as Tyrphostin AG 1478, is a potent and selective tyrosine kinase inhibitor that primarily targets the epidermal growth factor receptor (EGFR).
This compound has been extensively studied for its potential therapeutic applications in various disease models, particularly in the field of cancer research.
The EGFR is a receptor tyrosine kinase that plays a crucial role in cell signaling pathways involved in cell proliferation, survival, and differentiation.
Inhibition of EGFR by AG-1478 has been shown to disrupt these signaling cascades, making it a promising candidate for the treatment of EGFR-driven cancers, such as non-small cell lung cancer, head and neck cancer, and certain types of breast cancer.
In addition to its effects on EGFR, AG-1478 has also been investigated for its interactions with other signaling pathways.
For instance, it has been found to inhibit the phosphoinositide 3-kinase (PI3K) pathway, which is another important cellular signaling network implicated in cancer progression.
Compounds like LY294002, which target the PI3K pathway, have been used in combination with AG-1478 to explore synergistic effects in various preclinical models.
To optimize the use of AG-1478 in research settings, the PubCompare.ai platform offers a powerful tool for researchers.
This AI-driven platform allows researchers to locate the best protocols from the literature, preprints, and patents, enabling them to improve the reproducibility and accuracy of their AG-1478 studies.
By utilizing this platform, researchers can avoid common tyopss and ensure that their findings are robust and reliable.
When working with AG-1478, researchers often use cell culture systems, where the compound is dissolved in solvents like dimethyl sulfoxide (DMSO) and added to the culture medium, along with other growth factors like epidermal growth factor (EGF) and fetal bovine serum (FBS), to study its effects on cell biology and signaling pathways.