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Alexa 350

Alexa 350 is a voice-controlled virtual assistant developed by Amazon.
It is designed to provide users with a wide range of functionalities, including voice-based control of smart home devices, access to information and entertainment, and integration with various third-party services.
The Alexa 350 platform is known for its natural language processing capabilities, allowing users to issue voice commands and receive responses in a conversational manner.
It is commonly found in Amazon's Echo line of smart speakers, as well as in various other devices and appliances.
The Alexa 350 is a versatil and increasingly popular tool for voice-based interaction and automation in the smart home and beyond.

Most cited protocols related to «Alexa 350»

Measuring DNA Replication Dynamics using SMARD

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Publication 2009

3-(triethoxysilyl)propylamine Alexa 350 alexa 568 alexa fluor 488 Alkalies Antibodies Antibodies, Anti-Idiotypic Avidin Biological Assay Biotin Buffers Cells Cloning Vectors Ethanol Glutaral Goat Immunoglobulins isolation Mice, House Microscopy Molecular Probes Monoclonal Antibodies neutravidin Nucleotides Pulses Telomere

Immunofluorescence Analysis of Mouse Spinal Cord

After terminal anesthesia by barbiturate overdose mice were perfused transcardially with 10% formalin (Sigma). Spinal cords were removed, post-fixed overnight, and cryoprotected in buffered 30% sucrose for 48 hours. Frozen sections (30μm horizontal) were prepared using a cryostat microtome (Leica) and processed for immunofluorescence as described^{16 (link)–18 (link)}. Primary antibodies were: rabbit anti-GFAP (1:1000; Dako, Carpinteria, CA); rat anti-GFAP (1:1000, Zymed Laboratories); goat anti-CTB (1:1000, List Biology Lab); rabbit anti-5HT (1:2000, Immunostar); goat anti-5HT (1:1000, Immunostar); mouse anti-CSPG^{22 (link)} (1:100, Sigma); rabbit-anti hemagglutinin (HA) (1:500 Sigma); mouse-anti HA (1:3000 Covance); sheep anti-BrdU (1:6000, Maine Biotechnology Services, Portland, ME); rabbit anti-laminin (1:80, Sigma, Saint Louis, MO); guinea pig anti-NG2 (CSPG4) (Drs. E.G. Hughes and D.W. Bergles^{57 (link)}, Baltimore, MA); goat anti-aggrecan (1:200, NOVUS); rabbit anti-brevican (1:300, NOVUS); mouse anti-neurocan (1:300, Milipore); mouse anti-phosphacan (1:500, Sigma); goat anti-versican (1:200, NOVUS); rabbit anti-neurglycan C (CSPG5) (1:200, NOVUS). Fluorescence secondary antibodies were conjugated to: Alexa 488 (green) or Alexa 350 (blue) (Molecular Probes), or to Cy3 (550, red) or Cy5 (649, far red) all from (Jackson Immunoresearch Laboratories). Mouse primary antibodies were visualized using the Mouse-on-Mouse detection kit (M.O.M. ®, Vector). BDA tract-tracing was visualized with streptavidin-HRP plus TSB Fluorescein geen or Tyr-Cy3 (Jackson Immunoresearch Laboratories). Nuclear stain: 4′,6′-diamidino-2-phenylindole dihydrochloride (DAPI; 2ng/ml; Molecular Probes). Sections were coverslipped using ProLong Gold anti-fade reagent (InVitrogen, Grand Island, NY). Sections were examined and photographed using deconvolution fluorescence microscopy and scanning confocal laser microscopy (Zeiss, Oberkochen, Germany).

Anderson M.A., Burda J.E., Ren Y., Ao Y., O’Shea T.M., Kawaguchi R., Coppola G., Khakh B.S., Deming T.J, & Sofroniew M.V. (2016). Astrocyte scar formation aids CNS axon regeneration. Nature, 532(7598), 195-200.

Publication 2016

Aggrecans Alexa 350 Anesthesia Antibodies barbiturate Brevican Bromodeoxyuridine Cavia porcellus Cloning Vectors CSPG4 protein, human DAPI Domestic Sheep Drug Overdose Fluorescein Fluorescent Antibody Technique Formalin Frozen Sections Glial Fibrillary Acidic Protein Goat Gold Hemagglutinin Laminin Mice, House Microscopy, Confocal Microscopy, Fluorescence Microtomy Molecular Probes Neurocan Novus Protein Tyrosine Phosphatase, Receptor Type Z Rabbits Spinal Cord Stains Streptavidin Sucrose Versicans

Genetic Lineage Tracing of Hepatocytes

Mice bearing the (ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo} (Muzumdar et al., 2007 (link)) (“ROSA^mT-mG”) allele and Tg(Alb-cre)21Mgn (Postic et al., 1999 (link)) (“albCre”) mice were purchased from Jackson Laboratories (stocks 007576 and 003574, respectively). ROSA^mT-mG (Muzumdar et al., 2007 (link)) is a “knock-in” of the ubiquitously expressed ROSA26 locus (Soriano, 1999 (link)). ROSA^mT-mG contains a floxed cistron encoding a membrane-targeted red fluorescent protein (tdTomato) followed by a cistron encoding a membrane-targeted GFP (Muzumdar et al., 2007 (link)). In its non-recombined state, ROSA^mT-mG causes red fluorescence in all cells. Following Cre-dependent recombination, cells convert from red- to green-fluorescence (Muzumdar et al., 2007 (link)).
In situ hybridizations used a digoxygenin-labeled cRNA probe recognizing +1553 to +1865 of Alb mRNA (NM_009654). The procedure obliterates GFP and tdTomato so within figures, in situ hybridization and fluorescence images are of different sections from the same liver. For detection of intracellular Alb protein, anesthetized pups were sacrificed and perfused via cardiac puncture with 2 ml saline followed by 2 ml 4% paraformaldehyde/PBS. Immunofluorescence used goat-anti-mouse albumin antibody (Bethyl #A90-134A) and Alexa Fluor 350-labeled donkey-anti-goat secondary antibody (Molecular Probes #A21081). Animal protocols were approved by the Montana State University Institutional Animal Care and Use Committee.

Weisend C.M., Kundert J.A., Suvorova E.S., Prigge J.R, & Schmidt E.E. (2009). CRE ACTIVITY IN FETAL albCre MOUSE HEPATOCYTES: UTILITY FOR DEVELOPMENTAL STUDIES. Genesis (New York, N.Y. : 2000), 47(12), 789-792.

Publication 2009

Albumins Alexa 350 Alleles Animals Antibodies, Anti-Idiotypic Cells Cistron Complementary RNA Equus asinus Erythrocytes Fluorescence Fluorescent Antibody Technique Goat Heart In Situ Hybridization Institutional Animal Care and Use Committees Liver Membrane Proteins Molecular Probes Mus paraform Proteins Protoplasm Punctures Recombination, Genetic RNA, Messenger Rosa Saline Solution tdTomato Tissue, Membrane

Dual-Labeling Immunohistochemistry for Fos and CTb

Sections at 200 μm intervals (80 μm intervals in the area of VTA CTB injection sites) were stained for Fos and CTb using previously described procedures (Sartor and Aston-Jones, 2012 (link)). Briefly, sections were incubated in a rabbit anti-Fos primary antibody overnight (1:2000; Santa Cruz Biotechnology), followed by 2 h in a donkey anti-rabbit secondary (1:500, Jackson ImmunoResearch Laboratories), amplified with the avidin biotin complex (ABC; Vector Labs) method (1:500), and visualized with 3, 3′ diaminobenzidine (DAB) + nickel ammonium sulfate to yield a blue-black nuclear reaction product. CTb was subsequently visualized with a goat anti-CTb primary antibody (1:20,000; List Biological Laboratories), donkey anti-goat secondary (1:500, Jackson ImmunoResearch Laboratories), ABC, and DAB to yield a brown somatic stain in VTA-projecting cells.
Sections from the same brains at ~2.8 and 3.2 mm caudal of bregma (near the center of the orexin field of the hypothalamus) were also stained for Fos, CTb, and orexin_A using fluorescent immunohistochemistry. Rabbit anti-Fos (1:2500; Santa Cruz Biotechnology), mouse anti-CTb (1:500; AbD Serotec), and goat anti-orexin_A (1:500; Jackson ImmunoResearch Laboratories) primaries were mixed, and sections were incubated overnight at room temperature. After washing, sections were incubated in donkey anti-rabbit Alexa Fluor 594 (1:500; Invitrogen) and donkey anti-mouse Alexa Fluor 488 (1:500; Invitrogen) secondary antibodies at room temperature for 4 h, washed, then incubated in biotinylated donkey anti-goat secondary antibody (1:100; Jackson ImmunoResearch Laboratories) for 3 d at 4°C. Finally, sections were incubated in a streptavidin-conjugated Alexa Fluor 350 chromogen (1:100; Invitrogen) for 4 h at room temperature. Slices were mounted and coverslipped with Citifluor mounting medium, and stored at 4°C until photographed at 20× magnification with a Leica fluorescent microscope.

Mahler S.V, & Aston-Jones G.S. (2012). Fos Activation of Selective Afferents to Ventral Tegmental Area during Cue-Induced Reinstatement of Cocaine Seeking in Rats. The Journal of Neuroscience, 32(38), 13309-13325.

Publication 2012

Alexa 350 Alexa594 alexa fluor 488 ammonium nickel sulfate Antibodies Antibodies, Anti-Idiotypic Avidin azo rubin S Biopharmaceuticals Biotin Cloning Vectors Diploid Cell Equus asinus Goat HCRT protein, human Hypothalamus Immunohistochemistry Mice, House Microscopy POU3F2 protein, human Rabbits Streptavidin Vascular Access Ports

Comprehensive Flow Cytometry Immunophenotyping

Antibodies used for flow cytometry were from eBioscience (San Diego, CA) unless otherwise indicated. Cell suspensions were incubated with FITC-labeled anti-GL7 (BD Biosciences, San Jose, CA), PercP-Cy5.5-labeled anti-B220, APC-labeled anti-mouse IgM, Alexa Fluor 700-labeled anti-CD38, and APC-eFluor780-labeled anti-CD4, CD8, F480, CD11c, and Gr-1 antibodies. The cells were then fixed in 2% formaldehyde for 20 minutes in ice, permeabilized with 0.5% saponin (Sigma), and incubated with biotin-labeled anti-IgG1 (A85-1), IgG2c (5.7), IgG2b (R12-3), IgG3(R40-82) (all from BD Biosciences), IgA (RMA-1), and IgE (RME-1) antibodies (both from Biolegend, San Diego, CA) followed by Pacific Blue-labeled streptavidin and Pacific Orange labeled anti-mouse Ig H+L antibody (both from Invitrogen). In some cases, FITC-labeled anti-IgD, CD80, CD73, VLA-4, CD16/32 (BD Biosciences), or CD44 antibodies were used instead of anti-GL7 antibody. In other cases, FITC-labeled anti-B220, PercP-Cy5.5-labeled anti-IgM, and APC-labeled anti-TACI or anti-BAFFR antibodies were substituted for FITC-labeled anti-GL7, PercP-Cy5.5-labeled anti-B220, and APC-labeled anti-mouse IgM antibodies. For detection of BrdU, samples were subjected to an additional fixation with Cytofix/Cytoperm^™ (BD Biosciences), followed by incubation with 1 mg/ml DNAse I (Sigma) for 60 minutes at 37°C, culture supernatant containing 24G2 antibody plus 1% rat serum for 10 minutes at 4°C, and FITC-labeled anti-BrdU (BD Biosciences) for 30 minutes at 4°C. For marginal zone and B1 cell detection, cell suspensions were prepared in the absence of collagenase to prevent cleavage of CD23 (5 (link)), and incubated with FITC-labeled anti-CD23 antibody (BD), PercP-Cy5.5-labeled anti-B220, APC-labeled anti-CD93, Alexa Fluor 650-labeled anti-IgM, eFluor-450-labeled anti-CD21, biotin-labeled CD43 (BD), and APC-eFluor780-labeled anti-CD4, CD8, F480, CD11c, and Gr-1 antibodies, followed by an incubation with V500-labled streptavidin (BD). The cells were then fixed in 2% formaldehyde, permeabilized with 0.5% saponin (Sigma), and incubated with Alexa Fluor 350-labeled anti-mouse Ig H+L antibody. Flow cytometry was performed on a 4-laser (355nm, 405nm, 488nm, 633nm) LSR II device (BD Biosciences) and analyzed with FlowJo software (Tree Star, Ashland, OR). Fluorescent AccuCheck counting beads (Invitrogen) were used to calculate total numbers of live lymphocytes in the column bound and flow through suspensions.

Pape K.A., Taylor J.J., Maul R.W., Gearhart P.J, & Jenkins M.K. (2011). Different B cell populations mediate early and late memory during an endogenous immune response. Science (New York, N.Y.), 331(6021), 1203-1207.

Publication 2011

Alexa 350 Anti-Antibodies anti-IgD anti-IgM Antibodies Antibodies, Anti-Idiotypic Be1 Cells Biotin Bromodeoxyuridine CD44 protein, human Cells Collagenase CY5.5 cyanine dye Cytokinesis Deoxyribonucleases Flow Cytometry Fluorescein-5-isothiocyanate Formaldehyde IgG1 IgG2B IgG3 Integrin alpha4beta1 lumiliximab Lymphocyte Count Medical Devices Mus NT5E protein, human Saponin Serum SPN protein, human Streptavidin TNFRSF13B protein, human Trees

Most recents protocols related to «Alexa 350»

Myosin Heavy Chain Isoform Identification

The antibodies for three myosin heavy chain (MyHC) isoforms (MyHC1, MyHC2A, and MyHC2X) and laminin were used as described by Riaz et al., 2016 (link). Briefly, cryosections were stained with rabbit anti‐laminin (1:1000, Sigma-Aldrich, L9393) and mouse anti‐6H1 (1:5, DSHB; AB_2314830) detecting MyHC2X, for two hours at room temperature. Following the PBST washing, the secondary antibodies goat anti‐rabbit‐conjugated‐Alexa Fluor 750 (1:1000, Thermo Fisher Scientific, A21039) and goat anti‐mouse‐conjugated‐Alexa Fluor 488 (1:1000, A11001, Thermo Fisher Scientific) were incubated for an hour at room temperature. After PBST washing, sections were incubated overnight at four degrees with a mix of fluorescently conjugated monoclonal antibodies: BA‐D5‐conjugated‐Alexa Fluor 350 (1:600, DSHB, AB_2235587) and SC‐71‐conjugated‐Alexa Fluor 594 (1:700, DSHB, AB_2147165), detecting MyHC1 and MyHC2A, respectively. Lastly, after washing with PBST, the cryosections were mounted with ProLong Gold antifade reagent (P36930, Thermo Fisher Scientific) and stored at four degrees prior to imaging.

Abbassi-Daloii T., el Abdellaoui S., Voortman L.M., Veeger T.T., Cats D., Mei H., Meuffels D.E., van Arkel E., 't Hoen P.A., Kan H.E, & Raz V. (2023). A transcriptome atlas of leg muscles from healthy human volunteers reveals molecular and cellular signatures associated with muscle location. eLife, 12, e80500.

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Publication 2023

Alexa 350 Alexa594 alexa fluor 488 Alexa Fluor 750 Antibodies Cryoultramicrotomy Goat Gold Laminin Mice, House Monoclonal Antibodies Myosin Heavy Chains Protein Isoforms Rabbits

Chromatin Spread Preparation and Staining

Spermatocyte and oocyte chromatin spreads were prepared as previously described [78 (link)–80 (link)]. Primary antibodies and dilution used for immunolabeling are presented in S4 Table. Secondary antibodies against human, rabbit, rat, mouse, and guinea pig IgG and conjugated to Alexa 350, 488, 568, or 633 (Life Technologies) were used at a 1:500 dilution.
Images from chromatin spread preparations were captured using a Zeiss CellObserver Z1 microscope linked to an ORCA-Flash 4.0 CMOS camera (Hamamatsu). Testis sections stained with H&E staining were captured using a Zeiss AxioImager A2 microscope linked to an AxioCam ERc5s camera, or Keyence BZ-X800 fluorescence microscope. Images were analyzed and processed using ZEN 2012 blue edition imaging software (Zeiss) or with BZ-X800 Viewer and Analyzer software (Keyence).

Martinez-Garcia M., Naharro P.R., Skinner M.W., Baran K.A., Lascarez-Lagunas L.I., Nadarajan S., Shin N., Silva-García C.G., Saito T.T., Beese-Sims S., Diaz-Pacheco B.N., Berson E., Castañer A.B., Pacheco S., Martinez-Perez E., Jordan P.W, & Colaiácovo M.P. (2023). GRAS-1 is a novel regulator of early meiotic chromosome dynamics in C. elegans. PLOS Genetics, 19(2), e1010666.

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Publication 2023

Alexa 350 Antibodies Cavia Chromatin Chronic multifocal osteomyelitis Homo sapiens Mice, House Microscopy Microscopy, Fluorescence Oocytes Orcinus orca Rabbits Spermatocytes Technique, Dilution Testis

Multimodal Cellular Imaging Protocols

To visualize actin, cover slips were incubated 30 min in phalloidin-fluorescein dye (2 µM, Sigma Aldrich, P5282).
To visualize cellular membranes, cover slips were incubated with Wheat Germ Agglutinin (WGA), Alexa Fluor™ 350 Conjugate (10 µg/ml, ThermoFisher, W11263) for 2 h at 37 °C.
To visualize neutral lipids, cover slips were incubated with BODIPY 493/503 (1:100, ThermoFisher, D3922) for 30 min at 37 °C.

Zyśk M., Beretta C., Naia L., Dakhel A., Påvénius L., Brismar H., Lindskog M., Ankarcrona M, & Erlandsson A. (2023). Amyloid-β accumulation in human astrocytes induces mitochondrial disruption and changed energy metabolism. Journal of Neuroinflammation, 20, 43.

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Publication 2023

4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene Actins Alexa 350 Fluorescein Lipids Phalloidine Plasma Membrane Wheat Germ Agglutinins

Immunofluorescence and Immunohistochemistry Protocol

Cells were fixed with 4% paraformaldehyde for 20 min and permeabilized using 0.25% Triton X-100 for 20 min at room temperature. Use quick antigen retrieval solution for cell section (Solarbio) according to the manufacturer’s protocol to retrieve antigen fixed with 4% paraformaldehyde before blocking. Additionally, Cells were also fixed with −20 °C pre-cooled methanol for 5 min to obtain a better resolution of microtubule staining. After blocking with 1% BSA supplemented with 10% goat serum, the cells were incubated with the primary antibodies overnight at 4 °C. After washing with TBS, the cells were incubated with secondary antibodies conjugated with Alexa-350, Alexa-488, or Alexa-594 for 2.5 h at room temperature. Finally, the cells were stained with DAPI (Solarbio) to visualize the nuclei. Photos were taken under a confocal microscope (LSM-880 with Airyscan, Zeiss). Super-resolution Photos were taken under SP8 LIGHTNING confocal microscope (Leica). Shh-subtype medulloblastoma samples in OCT were washed with TBS three times and performed with antigen retrieval solution (Solarbio) for 5 min at room temperature according to the manufacturer’s protocol. After blocking with 1% BSA supplemented with 10% goat serum, the slices were incubated with the primary antibodies overnight at 4 °C. After washing with TBS, the slices were incubated with secondary antibodies conjugated with HRP for 2.5 h at room temperature. Finally, Using DAB to detect the signal according to the manufacturer’s protocol.

Song C., Zhang Y., Li Y., Bie J., Wang Z., Yang X., Li H., Zhu L., Zhang T., Chang Q, & Luo J. (2023). The phosphorylation of PHF5A by TrkA-ERK1/2-ABL1 cascade regulates centrosome separation. Cell Death & Disease, 14(2), 98.

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Publication 2023

Alexa 350 Alexa594 Antibodies Antigens Cell Nucleus Cells DAPI Goat Medulloblastoma Methanol Microscopy, Confocal Microtubules paraform Serum Triton X-100

Cloning and Tagging of Centrosomal Proteins

PCR-amplified human PHF5A, ABL1, and Nek2A were cloned into pcDNA 3.1 vectors (with -HA, -Flag, or -Myc-His tags). PCR-amplified human CETN2 were cloned into pEGFP-C2 vectors. PcDNA 3.1-Flag-PHF5A (all mutant) and pcDNA 3.1-HA-ABL1 (PP and K290R) were constructed using KOD-plus Mutagenesis Kit (TOYOBO). pLVX-TetOne-Puro-Flag-PHF5A (WT, Y36E, Y36F), pQCXIH-Flag-PHF5A, and pHBLV-CMVIE-IRES-Puro EGFP-CETN2 were constructed using ClonExpress II One Step Cloning Kit (Vazyme). ShRNA was constructed via the ligation of an oligonucleotide into a pLKO.1-Puro vector. The following shRNA target sequences were used: shRNA PHF5A CDS, 5′-CCAUCGGAAGACUGUGUGAAA-3′; shRNA PHF5A UTR, 5′-GCCUACUACUACCAGCAGAAA-3′; shRNA ABL1 UTR, 5′- CCAGCTCTACTACCTACGTTT-3′. The following antibodies were obtained from commercial sources: Anti-Flag (Sigma-Aldric, Cat#F-3165), Anti-GFP (Enogene, Cat#E12-009), Anti-pTyr (PY99) (Santa Cruz Biotechnology, Cat#sc-7020), Anti-GAPDH (Cell Signaling Technology, Cat#5174), Anti-HA (Thermo Fisher Scientific, Cat#26183), Anti-TrkA (Bioss, Cat#ba-0193R), Anti-PHF5A (Proteintech, Cat#15554-1-AP), Anti-ERK1/2 (Abcam, Cat#ab184699 and Zenbio, Cat#201246-4F3), Anti-pERK1/2 (Thr202/Tyr204) (Zenbio, Cat#301245), Anti-β-actin (Santa Cruz Biotechnology, Cat#sc-47778), Anti-AKT (Cell Signaling Technology, Cat#4685), Anti-pAKT (Ser473) (Cell Signaling Technology, Cat#4060), Anti-ABL1 (Cell Signaling Technology, Cat#2862), Anti-pABL1 (Tyr412) (Cell Signaling Technology, Cat#2865), Anti-γ-tubulin (mouse: Proteintech, Cat#66320-I-IG and rabbit: Abcam, Cat#ab179503), Anti-CEP250 (mouse: Santa Cruz Biotechnology, Cat#sc-390540 and rabbit: Proteintech, Cat#14498-1-AP), Donkey anti-rabbit Alexa Fluor 488 (Thermo Fisher Scientific, Cat#A21206), Goat anti-mouse Alexa Fluor 594 (Thermo Fisher Scientific, Cat#A32742), Goat anti-mouse Alexa Fluor 488 (Yeason, Cat# 33206ES60), Donkey anti-rabbit Alexa Fluor 350 (Bioss, Cat#bs-0295D-AF250).

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Publication 2023

Actins Alexa 350 Alexa594 alexa fluor 488 Antibodies Chloramphenicol O-Acetyltransferase Cloning Vectors Equus asinus GAPDH protein, human GIT1 protein, human Goat Homo sapiens Internal Ribosome Entry Sites Ligation Mitogen-Activated Protein Kinase 3 Mus Mutagenesis Oligonucleotides Rabbits Short Hairpin RNA Tubulin

Top products related to «Alexa 350»

Alexa fluor 350 by Thermo Fisher Scientific

Sourced in United States, Germany

Alexa Fluor 350 is a fluorescent dye used in various biological applications. It has an excitation maximum at 346 nm and an emission maximum at 442 nm, making it suitable for use in fluorescence-based experiments.

Alexa fluor 488 by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Germany, Japan, France, Italy, Canada, China, Spain, Switzerland, Denmark, Australia, Hungary, Belgium, Ireland, Israel, Netherlands, Moldova, Republic of, India, Austria, Czechia, Poland

Alexa Fluor 488 is a fluorescent dye used in various biotechnological applications. It has an excitation maximum at 495 nm and an emission maximum at 519 nm, producing a green fluorescent signal. Alexa Fluor 488 is known for its brightness, photostability, and pH-insensitivity, making it a popular choice for labeling biomolecules in biological research.

Alexa 350 by Thermo Fisher Scientific

Sourced in Germany

The Alexa 350 is a compact spectrophotometer designed for accurate and reliable measurement of UV-Vis absorbance. It features a wavelength range of 190-1100 nm and can be used for a variety of routine analysis applications in laboratories.

Prolong gold antifade reagent by Thermo Fisher Scientific

Sourced in United States, Germany, Japan, United Kingdom, Italy, Australia, Canada, France, Spain, China, Netherlands, Austria

ProLong Gold antifade reagent is a water-based mounting medium designed to retard the photobleaching of fluorophores in fluorescence microscopy applications. It is formulated to provide long-term preservation of fluorescent signals in fixed and immunolabeled samples.

Alexa fluor 350 phalloidin by Thermo Fisher Scientific

Sourced in United States

Alexa Fluor 350 phalloidin is a fluorescent dye that binds to F-actin, a component of the cytoskeleton in eukaryotic cells. It can be used to visualize the distribution and organization of actin filaments in cells.

Sc 71 by Developmental Studies Hybridoma Bank

Sourced in United States, United Kingdom

The SC-71 is a laboratory equipment designed for performing cell culture and hybridoma-related experiments. It functions as an incubator, providing a controlled environment for the growth and maintenance of cells. The device regulates temperature, humidity, and atmospheric conditions to support optimal cell culture conditions.

Hoechst 33342 by Thermo Fisher Scientific

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Hoechst 33342 is a fluorescent dye that binds to DNA. It is commonly used in various applications, such as cell staining and flow cytometry, to identify and analyze cell populations.

Propidium iodide by Thermo Fisher Scientific

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Propidium iodide is a fluorescent dye used in flow cytometry to stain and quantify DNA content in cells. It binds to DNA by intercalating between the bases. Propidium iodide is commonly used to distinguish viable from non-viable cells.

4 6 diamidino 2 phenylindole (dapi) by Thermo Fisher Scientific

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DAPI is a fluorescent dye used in microscopy and flow cytometry to stain cell nuclei. It binds strongly to the minor groove of double-stranded DNA, emitting blue fluorescence when excited by ultraviolet light.

4 6 diamidino 2 phenylindole (dapi) by Merck Group

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DAPI is a fluorescent dye that binds strongly to adenine-thymine (A-T) rich regions in DNA. It is commonly used as a nuclear counterstain in fluorescence microscopy to visualize and locate cell nuclei.

What are the key features and capabilities of the Alexa 350 virtual assistant?

The Alexa 350 is a powerful voice-controlled virtual assistant developed by Amazon. It offers a wide range of functionalities, including voice-based control of smart home devices, access to information and entertainment, and integration with various third-party services. The Alexa 350 is known for its natural language processing capabilities, allowing users to issue voice commands and receive responses in a conversational manner. It is commonly found in Amazon's Echo line of smart speakers, as well as in other devices and appliances, making it a versatile tool for voice-based interaction and automation.

How can PubCompare.ai help researchers optimize their use of the Alexa 350?

PubCompare.ai is a leading AI-driven platform that can assist researchers in optimizing their use of the Alexa 350. The platform allows you to screen protocol literature more efficiently, leveraging AI to pinpoint critical insights. PubCompare.ai can help researchers identify the most effective protocols related to the Alexa 350 for their specific research goals. The platform's AI-driven analysis can highlight key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy.

Are there any common usage challenges or limitations with the Alexa 350?

While the Alexa 350 is a powerful and versitile virtual assistant, there are some common usage challenges or limitations to be aware of. For example, users may encounter issues with the device's natural language processing capabilities, such as difficulty understanding accents or interpreting complex queries. Additionally, the Alexa 350 may have limitations in terms of its integration with certain third-party services or devices, and its functionality may vary depending on the user's location and the available features in their region.

What are the different variations or types of the Alexa 350?

The Alexa 350 is available in several different variations and device types. The most common form is the Amazon Echo line of smart speakers, which come in various sizes and design options. However, the Alexa 350 technology is also integrated into other devices, such as televisions, smart displays, and even some appliances and vehicles. Each variation may have slightly different capabilities or features, so it's important for users to research the specific model they are interested in to ensure it meets their needs.

What are some of the specific applications or use cases for the Alexa 350?

The Alexa 350 has a wide range of applications and use cases, making it a versatile tool for both personal and professional use. Some common applications include voice-controlled smart home automation, hands-free control of entertainment systems, access to information and news, setting reminders and alarms, and even integration with various productivity tools and services. The Alexa 350 can also be used for voice-based shopping, making it a convenient option for users who prefer a more natural and intuitive way of interacting with their devices.

More about "Alexa 350"

Alexa 350 is a powerful and versatile voice-controlled virtual assistant developed by Amazon.
It is designed to provide users with a wide range of functionalities, including voice-based control of smart home devices, access to information and entertainment, and seamless integration with various third-party services.
The Alexa 350 platform is renowned for its advanced natural language processing capabilities, allowing users to issue voice commands and receive responses in a conversational, user-friendly manner.
Alexa 350 is commonly found in Amazon's popular Echo line of smart speakers, as well as in a growing number of other compatible devices and appliances.
It is a valuable tool for voice-based interaction and automation in the smart home and beyond, offering users a convenient and hands-free way to control their environment, access information, and enjoy entertainment.
While Alexa 350 is the primary focus, it's worth noting that there are other related terms and concepts that may be of interest.
Alexa Fluor 350 and Alexa Fluor 488 are fluorescent dyes used in various scientific and medical applications, while ProLong Gold antifade reagent is a mounting medium often used in microscopy.
Alexa 350, SC-71, Hoechst 33342, and Propidium iodide are also related terms that may be relevant in certain contexts.
Whether you're looking to harness the power of Alexa 350 in your smart home, or you're conducting research involving related scientific tools and techniques, this information should provide a solid foundation for understanding the key aspects and capabilities of this innovative technology.