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Alexa fluor 546

Alexa Fluor 546 is a fluorescent dye commonly used in biological research.
It is a red-orange fluorescent dye with excitation and emission spectra that make it well-suited for a variety of applications, including fluorescence microscopy, flow cytometry, and protein labeling.
PubCompare.ai's AI-driven platform can help researchers optimize their Alexa Fluor 546 experiments by navigating the vast landscape of protocols from literature, pre-prints, and patents.
The platform allows users to compare and identify the best protocols and products to enhance experimental reproducibility and unlock the full potential of Alexa Fluor 546 in their research.
With PubCompare.ai's intelligent protocol comparison tools, scientists can discover the power of this versatile fluorescent dye and take their Alexa Fluor 546 research to the next level.

Most cited protocols related to «Alexa fluor 546»

1
Multimodal Neuronal Labeling Protocol
Cited 12 times
The following dyes were used: wheat germ agglutinin A594 (1:250, Molecular Probes), NeuroTrace fluorescent Nissl stain A640 (1:200, Molecular Probes), DAPI (300 nM, Molecular Probes), and Alexa Fluor 546 streptavidin (1:500, Molecular Probes). Primary antibodies included: mouse anti-Map2 (1:1000, Sigma), mouse anti-calbindin (1:1500, Sigma), goat anti-HSP60 (1:200, Santa Cruz), rabbit anti-Dendra2 (1:500, Evrogen), and mouse anti-α-actinin (1:100, Sigma). Secondary antibodies included biotinylated goat anti-mouse (Vector labs), Alexa Fluor 546 donkey anti-goat, Alexa Fluor 546 donkey anti-mouse, and Alexa Fluor 488 goat-anti-rabbit (1:500, Molecular probes).
Pham A.H., McCaffery J.M, & Chan D.C. (2012). Mouse lines with photo-activatable mitochondria (PhAM) to study mitochondrial dynamics. Genesis (New York, N.Y. : 2000), 50(11), 833-843.
Publication 2012
Actinin alexa fluor 488 Alexa fluor 546 Antibodies Calbindins Cloning Vectors DAPI Dyes Equus asinus Goat MAP2 protein, human Molecular Probes Mus Rabbits Stains Streptavidin Wheat Germ Agglutinins
2
Tracking Intracellular Protein Trafficking in Myotubes
Cited 12 times
To monitor intracellular trafficking of fluorescent fusion proteins, C2C12 cells or mg53−/− myotubes were cultured in glass-bottom dishes (Bioptechs) and transfected with plasmid DNA using standard techniques. For immunocytochemistry, FDB fibres were fixed using 100% ethanol at −20 °C for 5 min before anti-mouse MG53 rabbit polyclonal antibody was applied at a 1:200 dilution. Cells were washed and secondary antibodies coupled with fluorescent probes (goat anti-rabbit Alexa Fluor 488 or Alexa Fluor 546) were applied according to the manufacturer’s instructions (Molecular Probes). For the assay of acute live-cell membrane damage, transfected cells were mechanically damaged using a micropipette attached to a micromanipulator. Fluorescence images were captured using a BioRad 2100 Radiance laser scanning confocal microscope with a ×40 1.3NA oil immersion objective.
Cai C., Masumiya H., Weisleder N., Matsuda N., Nishi M., Hwang M., Ko J.K., Lin P., Thornton A., Zhao X., Pan Z., Komazaki S., Brotto M., Takeshima H, & Ma J. (2008). MG53 nucleates assembly of cell membrane repair machinery. Nature cell biology, 11(1), 56-64.
Publication 2008
A-A-1 antibiotic alexa fluor 488 Alexa fluor 546 Antibodies Antibodies, Anti-Idiotypic Biological Assay Cells Ethanol Fibrosis Fluorescence Fluorescent Probes Goat Hyperostosis, Diffuse Idiopathic Skeletal Immunocytochemistry Microscopy, Confocal Molecular Probes Mus Plasma Membrane Plasmids Protoplasm Rabbits Skeletal Myocytes Submersion Technique, Dilution
3
Quantifying Axon Growth and Oligodendrocyte Differentiation
Cited 11 times

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Publication 2010
Alexa fluor 546 Anti-Antibodies Axon Cells Immunoglobulins Microscopy Molecular Probes Monoclonal Antibodies Neurons Oligodendroglia Rabbits Tubulin
4
GABA Receptor Subunit Expression and Tagging
Cited 9 times
The cDNAs encoding human α1, β2, γ2S and enhanced cyan fluorescent protein (CFP)-or yellow fluorescent protein (YFP)-tagged GABAA receptor subunits (e.g. γ2SCFP, α1CFP or γ2SYFP subunit) were as described previously (Kang and Macdonald, 2004 (link); Kang et al., 2006 (link)). The γ2 subunit minigenes were generated by inserting the full length intron 8 of the γ2 gene between exons 8 and 9 of the γ2 subunit cDNA constructs. Since intron 8 contains the sequences for the γ2L subunit, which is subject to alternative splicing inside the cells, the term γ2 subunit minigene was used instead of either γ2L or γ2S subunit minigene in Figure 1. Similarly, we are not sure if truncated wild type or mutant γ2S or γ2L subunits were produced with expression of the intron 8 minigenes, and therefore, we refer to γ2 and γ2(Q351X) subunits when the subunits were a product of minigene expression. We used γ2S or γ2L subunit when referring to the cDNA constructs for the rest of study. The ecliptic pHluorin (a pH-sensitive GFP variant)-tagged rat γ2L (γ2LpH) subunit was kindly provided by Dr. Stephen J. Moss. The FLAG (DYKDDDDK) and HA (YPYDVPDYA) epitopes were inserted between amino acids 4 and 5 in the amino terminus of the protein. Rat γ2 subunit siRNA, GFP-22 (CGG CAA GCT GAC CCT GAA GTT CAT), the scrambled negative control siRNA (Alexa Fluor 546; AAT TCT CCG AAC GTG TCA CGT) and primers were all synthesized by Qiagen. The anti-γ2 subunit siRNA sequence (AAG AAA TCT GAT GAT GAC TAT) was complementary to an upstream sequence of the rat γ2 subunit N-terminus that was interrupted in the YFP-tagged human γ2SYFP subunit sequence.
Kang J.Q., Shen W, & Macdonald R.L. (2009). The GABRG2 Mutation, Q351X, Associated with Generalized Epilepsy with Febrile Seizures Plus, Has Both Loss of Function and Dominant-Negative Suppression. The Journal of Neuroscience, 29(9), 2845-2856.
Publication 2009
Alexa fluor 546 Amino Acids Amino Acid Sequence Cells DNA, Complementary enhanced cyan fluorescent protein Epitopes Exons GABA-A Receptor Genes Homo sapiens Introns Mosses NR4A2 protein, human Oligonucleotide Primers PHluorin Proteins Protein Subunits RNA, Small Interfering
5
Antibody Isotyping for MOG and AQP4
Cited 7 times
Serum antibodies to MOG and AQP4 were analyzed in a subgroup of 15 patients for IgG1-IgG4 isotypes via our live cell staining IF assay using MOG or AQP4 transfected cells. After blocking with goat IgG, the transfected cells were incubated with the pre-absorbed serum samples (1:20 and 1:40 dilution) for one hour. Subsequently, cells were washed and incubated with mouse monoclonal anti-human IgG1-IgG4 isotype antibodies for 30 minutes (Sigma-Aldrich, 1:100 dilution in PBS/10% FCS), followed by detection using Alexa Fluor® 546 goat anti-mouse IgG (Invitrogen) for 30 minutes. Dead cells were excluded by DAPI staining and analysis was performed by three independent investigators (SM, KS and VG).
Mader S., Gredler V., Schanda K., Rostasy K., Dujmovic I., Pfaller K., Lutterotti A., Jarius S., Di Pauli F., Kuenz B., Ehling R., Hegen H., Deisenhammer F., Aboul-Enein F., Storch M.K., Koson P., Drulovic J., Kristoferitsch W., Berger T, & Reindl M. (2011). Complement activating antibodies to myelin oligodendrocyte glycoprotein in neuromyelitis optica and related disorders. Journal of Neuroinflammation, 8, 184.
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Publication 2011
Alexa fluor 546 Anti-Antibodies anti-IgG Antibodies Biological Assay Cells DAPI Goat Homo sapiens IgG1 IgG4 Immunoglobulin Isotypes Mus Patients Serum Technique, Dilution

Most recents protocols related to «Alexa fluor 546»

1
Analyzing C2C12 Cell Morphology on Nanofibers
The morphology of the C2C12 cells cultivated onto CA and CA@A nanofibers was determined by i) SEM and ii) F-actin staining.
For SEM analysis, C2C12 cells were seeded onto CA and CA@A nanofibers and cultivated at 37°C and 5% CO2 in GM. After 2 and 7 days of culturing, samples were fixed in 2.5% glutaraldehyde for 6 h at RT. Samples were then rinsed with distilled water and gradually dehydrated in two increasing series of ethyl alcohol (35%, 50%, 70%, 85%, 95% and 100% for 15 min/bath). Samples were metalized with gold and visualized using a Quanta 200 FEG SEM (FEI, Hillsboro, USA).
For F-actin staining, C2C12 cells were seeded onto CA and CA@A nanofibers and cultivated at 37°C and 5% CO2 for 7 days in GM. After washing in PBS, cells were fixed in 3.7% formaldehyde for 15 min at RT. Samples were permeabilized in 0.1% Triton-X100 in PBS for 10 min at RT, washed with PBS, and incubated with 0.2 μg/mL Alexa Fluor 546 Phalloidin (Thermo Fisher) in PBS, for 30 min at RT. Next, cell nuclei were counterstained with DAPI (diluted to 1:1,000 in PBS) for 20 min at RT. Images were obtained in a Zeiss fluorescence microscope.
dos Santos A.E., Cotta T., Santos J.P., Camargos J.S., do Carmo A.C., Alcântara E.G., Fleck C., Copola A.G., Nogueira J.M., Silva G.A., Andrade L.D., Ferreira R.V, & Jorge E.C. (2023). Bioactive cellulose acetate nanofiber loaded with annatto support skeletal muscle cell attachment and proliferation. Frontiers in Bioengineering and Biotechnology, 11, 1116917.
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Publication 2023
Alexa fluor 546 Bath Cell Nucleus Cells DAPI Ethanol F-Actin Formaldehyde Glutaral Gold Microscopy, Fluorescence Phalloidine Triton X-100
2
Drosophila Synapse Immunostaining and Imaging
Third instar larvae were dissected in HL3 buffer and subsequently fixed in HL3 + 3.7%PFA for 20 min. Tissue was permeabilized using 1× PBS with 0.2% Triton-X and 5% BSA. Staining was performed using the following probes/antibodies: horseradish peroxidase (HRP; Jackson ImmunoResearch Laboratories, 1:250), Disc Large 1 (4F3, DHSB, 1:50), mouse monoclonal YARS1 (Abnova, 1:500), rabbit polyclonal GFP (Invitrogen, 1:2000), rabbit polyclonal RFP (Abcam, 1:250), mouse monoclonal Brp (nc82, DHSB, 1:100), mouse monoclonal FasII (1D4, DHSB, 1:250), mouse monoclonal Synapsin (SynORF1, 3C11, DHSB, 1:500). Alexa Fluor®−488 and Alexa Fluor®−546 secondary antibodies were used (Invitrogen, 1:1000). Muscle 6/7 of abdominal hemisegments 3 and 4 were imaged.
Laser scanning confocal microscopy was performed on a Carl Zeiss LSM700 microscope equipped with a 20× Plan-Apochromat (0.8 NA) or 63× Plan-Apochromat (1.4 NA) objective. Super-resolution structured illumination microscopy was performed on a Zeiss ELYRA S.1 microscope equipped with a 63× Plan-Apochromat objective (1.4 NA). For a description of methods used to calculate Lifeact-RFP distribution at synaptic boutons, please see Supplementary Fig. 7.
Ermanoska B., Asselbergh B., Morant L., Petrovic-Erfurth M.L., Hosseinibarkooie S., Leitão-Gonçalves R., Almeida-Souza L., Bervoets S., Sun L., Lee L., Atkinson D., Khanghahi A., Tournev I., Callaerts P., Verstreken P., Yang X.L., Wirth B., Rodal A.A., Timmerman V., Goode B.L., Godenschwege T.A, & Jordanova A. (2023). Tyrosyl-tRNA synthetase has a noncanonical function in actin bundling. Nature Communications, 14, 999.
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Publication 2023
Abdominal Muscles alexa fluor 488 Alexa fluor 546 Antibodies Buffers Larva Light Microscopy Mice, House Microscopy Microscopy, Confocal, Laser Scanning Presynaptic Terminals Rabbits Synapsins Tissues
3
Immunostaining of Microglia and Neural Markers
Microglia were fixed for 20 min with 4% paraformaldehyde in 0.1 M PBS after treatment with neural debris. The cells were rinsed with PBS and incubated sequentially in: (i) blocking solution (PBS containing 5% normal goat serum and 0.1% Triton X-100), (ii) primary antibodies [MAP2 (1:1000; BioLegend, catalog no. 801801), myelin CNPase (1:1000; BioLegend, catalog no. 836404), and Iba-1 (1:500; Cell Signaling Technology, catalog no.17198S)], (iii) PBS, (iv) secondary antibody(ies) [goat anti-mouse Alexa Fluor 546 (1:1000; Invitrogen, catalog no. A11030) and goat anti-rabbit Alexa Fluor 633 (1:1000; Invitrogen, catalog no. A21071)], and (v) DAPI nuclei marker (1:1000; Sigma-Aldrich). Chamber slides were examined with a Leica confocal microscope.
Ritzel R.M., Li Y., Jiao Y., Lei Z., Doran S.J., He J., Shahror R.A., Henry R.J., Khan R., Tan C., Liu S., Stoica B.A., Faden A.I., Szeto G., Loane D.J, & Wu J. (2023). Brain injury accelerates the onset of a reversible age-related microglial phenotype associated with inflammatory neurodegeneration. Science Advances, 9(10), eadd1101.
Publication 2023
2',3'-Cyclic-Nucleotide Phosphodiesterases Aftercare Alexa fluor 546 Antibodies Cell Nucleus Cells DAPI Goat Immunoglobulins METAP2 protein, human Microglia Microscopy, Confocal Mus Myelin Nervousness paraform Rabbits Serum Triton X-100
4
Immunostaining of Frozen Tissue Sections
The frozen tissue sections were fixed in −20°C acetone for 10 min and washed in phosphate-buffered saline (PBS). Background was blocked with 2% bovine serum albumin, 0.3% Triton-X100, and 5% goat serum (Invitrogen, Carlsbad, CA) in PBS for 30 min at room temperature (RT). Three primary antibodies were combined in each protocol, diluted according to Supplementary Table S2. Primary antibodies were incubated at 4°C overnight. The sections were washed, and the following secondary antibodies were added for 1 h at RT: goat anti-mouse Alexa Fluor 546, goat anti-rabbit Alexa Fluor 546, or goat anti-rabbit Alexa Fluor 647 (Invitrogen). To enable the use of two mouse primary antibodies, the cTnT antibody was conjugated with Alexa 488 fluorochrome using Zenon Kit (Invitrogen). The samples were fixed using Histofix (Histolab, Gothenburg) for 15 min, washed, and mounted with prolong gold antifade reagent with nuclei staining 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen). Corresponding isotype controls for primary antibodies and did not show specific staining.
Sjölin L., Jonsson M., Orback C., Oldfors A., Jeppsson A., Synnergren J., Rotter Sopasakis V, & Vukusic K. (2023). Expression of Stem Cell Niche-Related Biomarkers at the Base of the Human Tricuspid Valve. Stem Cells and Development, 32(5-6), 140-151.
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Publication 2023
Acetone Alexa fluor 546 Alexa Fluor 647 Antibodies Cell Nucleus Fluorescent Dyes Frozen Sections Goat Gold Immunoglobulin Isotypes Immunoglobulins Mice, House Phosphates Rabbits Saline Solution Serum Serum Albumin, Bovine Tissues Triton X-100
5
Immunostaining Protocol for Spinal Cord and Neuromuscular Junctions
C5–C7 spinal segments or musculocutaneous nerves were cut into 15-µm-thick frozen sections for immunostaining. The blocking buffer was composed of 5% goat serum and 3% bovine serum albumin diluted in 0.1 M phosphate buffer saline (PBS). Signal was detected with Alexa fluor 546 or 488 coupled secondary antibodies (1:1000, Invitrogen). Primary antibodies were: goat anti- choline acetyltransferase (ChAT, 1:500, ab144p, Millipore), chicken anti-β-gal (1:500, ab9361, Abcam), rabbit anti-Calretinin (1:300, ab702, Abcam), mouse anti-Parvalbumin (1:1000, Mab1572, Millipore), rabbit anti-CAMKII (1:500, ab104224, Abcam), rabbit anti-vesicular GABA transporter (VGAT; 1:800, NO131013, Synaptic Systems), mouse anti-vesicular glutamate transporter 1 (vGlut1; 1:1000, Mab5502, Millipore), rat anti-major histocompatibility complex 1 (MHC1; 1:300, sc-59199, Santa Cruz), rabbit anti-glial fibrillary acidic protein (GFAP; 1:1000, AB7260, Abcam), rabbit anti-Iba1(1:1000, 019–19,741, Wako), and rabbit anti-Oligo2 (1:500, ab9610, Merck Millipore).
On day 50 after BPA, the biceps were collected and 7-µm horizontal sections were prepared with a sliding microtome (Leica, Germany) and double stained with rabbit anti-NF200 (1:500, n4142, Sigma) and α-BT (1:1000, Molecular probes, USA) to visualize neuromuscular junctions (NMJs).
Yu L., Liu M., Li F., Wang Q., Wang M., So K.F., Qu Y, & Zhou L. (2023). Celsr2 Knockout Alleviates Inhibitory Synaptic Stripping and Benefits Motoneuron Survival and Axon Regeneration After Branchial Plexus Avulsion. Molecular Neurobiology, 60(4), 1884-1900.
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Publication 2023
Alexa fluor 546 Antibodies Buffers Calmodulin-Dependent Protein Kinase II Calretinin Chickens Choline O-Acetyltransferase Frozen Sections Glial Fibrillary Acidic Protein Goat Major Histocompatibility Complex Mice, House Microtomy Molecular Probes Nerves, Musculocutaneous Neuromuscular Junction OLIG2 protein, human Parvalbumins Phosphates Rabbits Saline Solution Serum Serum Albumin, Bovine vesicular GABA transporter Vesicular Glutamate Transport Protein 1

Top products related to «Alexa fluor 546»

1
Alexa fluor 546 by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, Japan, France, Italy, Denmark, China
Alexa Fluor 546 is a fluorescent dye used in various life science applications. It has an absorption maximum at 556 nm and an emission maximum at 573 nm, making it suitable for detection in the orange-red region of the visible spectrum.
2
Alexa fluor 488 by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, Japan, France, Italy, Canada, China, Spain, Switzerland, Denmark, Australia, Hungary, Belgium, Ireland, Israel, Netherlands, Moldova, Republic of, India, Austria, Czechia, Poland
Alexa Fluor 488 is a fluorescent dye used in various biotechnological applications. It has an excitation maximum at 495 nm and an emission maximum at 519 nm, producing a green fluorescent signal. Alexa Fluor 488 is known for its brightness, photostability, and pH-insensitivity, making it a popular choice for labeling biomolecules in biological research.
3
4 6 diamidino 2 phenylindole (dapi) by Thermo Fisher Scientific
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DAPI is a fluorescent dye used in microscopy and flow cytometry to stain cell nuclei. It binds strongly to the minor groove of double-stranded DNA, emitting blue fluorescence when excited by ultraviolet light.
4
Alexa fluor 546 phalloidin by Thermo Fisher Scientific
Sourced in United States, Germany, Italy, France
Alexa Fluor 546 phalloidin is a fluorescent dye conjugate used for labeling and visualizing actin filaments in cells. It binds to F-actin with high affinity and specificity, allowing for the detection and localization of the actin cytoskeleton in fixed and permeabilized samples.
5
4 6 diamidino 2 phenylindole (dapi) by Merck Group
Sourced in United States, Germany, Japan, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, Canada, Switzerland, Spain, Australia, Denmark, India, Poland, Israel, Belgium, Sweden, Ireland, Netherlands, Panama, Brazil, Portugal, Czechia, Puerto Rico, Austria, Hong Kong, Singapore
DAPI is a fluorescent dye that binds strongly to adenine-thymine (A-T) rich regions in DNA. It is commonly used as a nuclear counterstain in fluorescence microscopy to visualize and locate cell nuclei.
6
Triton x 100 by Merck Group
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Triton X-100 is a non-ionic surfactant commonly used in various laboratory applications. It functions as a detergent and solubilizing agent, facilitating the solubilization and extraction of proteins and other biomolecules from biological samples.
7
Alexa fluor 546 goat anti rabbit igg by Thermo Fisher Scientific
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Alexa Fluor 546 goat anti-rabbit IgG is a secondary antibody conjugate designed for use in fluorescence-based applications. It is made by conjugating Alexa Fluor 546 dye to goat-derived antibodies that are specific to rabbit immunoglobulin G (IgG). The Alexa Fluor 546 dye provides a bright, photostable fluorescent signal that can be detected using standard TRITC filter sets.
8
Hoechst 33342 by Thermo Fisher Scientific
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Hoechst 33342 is a fluorescent dye that binds to DNA. It is commonly used in various applications, such as cell staining and flow cytometry, to identify and analyze cell populations.
9
Bovine serum albumin (bsa) by Merck Group
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Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
10
Goat anti mouse alexa fluor 546 by Thermo Fisher Scientific
Sourced in United States, United Kingdom, China, Spain
Goat anti-mouse Alexa Fluor 546 is a secondary antibody conjugated with the Alexa Fluor 546 fluorescent dye. It is designed to detect and label mouse primary antibodies in various immunoassay techniques.

More about "Alexa fluor 546"

Alexa Fluor 546 is a versatile fluorescent dye that has become a staple in biological research.
This red-orange dye excites and emits at wavelengths that make it well-suited for a variety of applications, including fluorescence microscopy, flow cytometry, and protein labeling.
Researchers can harness the power of Alexa Fluor 546 to visualize and study a range of biological processes and structures.
Closely related to Alexa Fluor 488, another widely used fluorescent dye, Alexa Fluor 546 shares many of the same properties and applications.
Both dyes offer bright, photostable fluorescence that can be readily detected and quantified.
When combined with counterstains like DAPI or Hoechst 33342, Alexa Fluor 546 can be used to create multicolor labeling schemes, allowing researchers to visualize multiple cellular components or targets simultaneously.
Alexa Fluor 546 phalloidin, a conjugate of the dye and the actin-binding toxin phalloidin, is a popular tool for labeling and visualizing the actin cytoskeleton.
By binding to F-actin, Alexa Fluor 546 phalloidin provides a clear picture of the intricate network of actin filaments within cells.
This can be particularly useful for studying cell morphology, migration, and other dynamic cellular processes.
To optimize the use of Alexa Fluor 546 in research, scientists can turn to PubCompare.ai's AI-driven platform.
This innovative tool allows researchers to navigate the vast landscape of protocols from literature, preprints, and patents, comparing and identifying the best methods and products to enhance experimental reproducibility.
By leveraging PubCompare.ai's intelligent protocol comparison tools, scientists can unlock the full potential of Alexa Fluor 546 and take their research to new heights.
Whether you're studying protein localization, tracking cellular dynamics, or exploring a range of other biological phenomena, Alexa Fluor 546 and the power of PubCompare.ai can help you achieve your research goals.
With its versatility, brightness, and the support of cutting-edge tools, this fluorescent dye is a valuable asset in the modern biological laboratory.