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Alexa Fluor 555

Alexa Fluor 555 is a popular fluorescent dye used in a variety of biological applications, such as immunofluorescence, flow cytometry, and microscopy.
This bright, photostable dye emits orange-red fluorescence and is commonly conjugated to antibodies, proteins, and other biomolecules to enable visualization and detection.
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Most cited protocols related to «Alexa Fluor 555»

1
In vivo Multimodal Brain Imaging
In vivo multiphoton imaging of TMR-conjugated dextran and detection of endogenous IgG, fibrin, thrombin and Prussian blue deposits in brain tissue was performed as previously described14 (link). Detection of neuronal uptake of systemically administered Alexa fluor 555-conjugated cadaverine was performed as described15 (link).
BELL R.D., WINKLER E.A., SINGH I., SAGARE A.P., DEANE R., WU Z., HOLTZMAN D.M., BETSHOLTZ C., ARMULIK A., SALLSTROM J., BERK B.C, & ZLOKOVIC B.V. (2012). Apolipoprotein E controls cerebrovascular integrity via cyclophilin A. Nature, 485(7399), 512-516.
Publication 2012
Alexa Fluor 555 Brain Cadaverine Dextran ferric ferrocyanide Fibrin Neurons Thrombin Tissues
2
Enrichment and Detection of HD-CTCs
Blood specimens were rocked for 5 minutes before a white blood cell (WBC) count was measured using the Hemocue white blood cell system (HemoCue, Sweden). Based upon the WBC count, a volume of blood was subjected to erythrocyte lysis (ammonium chloride solution). After centrifugation, nucleated cells were re-suspended in PBS and attached as a monolayer on custom made glass slides. The glass slides are the same size as standard microscopy slides but have a proprietary coating that allows maximal retention of live cells. Each slide can hold approximately 3 million nucleated cells, thus the number of cells plated per slide depended on the patients WBC count.
For HD-CTC detection in cancer patients for this study, 4 slides are used as a test. The remaining slides created for each patient are stored at −80°C for future experiments. Four slides were thawed from each patient, then cells were fixed with 2% paraformaldehyde, permeabilized with cold methanol, and non-specific binding sites were blocked with goat serum. Slides were subsequently incubated with monoclonal anti-pan cytokeratin antibody (Sigma) and CD45-Alexa 647 (Serotec) for 40 minutes at 37°C. After PBS washes, slides were incubated with Alexa Fluor 555 goat anti-mouse antibody (Invitrogen) for 20 minutes at 37°C. Cells were counterstained with DAPI for 10 minutes and mounted with an aqueous mounting media.
Marrinucci D., Bethel K., Kolatkar A., Luttgen M., Malchiodi M., Baehring F., Voigt K., Lazar D., Nieva J., Bazhenova L., Ko A.H., Korn W.M., Schram E., Coward M., Yang X., Metzner T., Lamy R., Honnatti M., Yoshioka C., Kunken J., Petrova Y., Sok D., Nelson D, & Kuhn P. (2012). Fluid Biopsy in Patients with Metastatic Prostate, Pancreatic and Breast Cancers. Physical Biology, 9(1), 016003.
Publication 2012
Alexa Fluor 555 Antibodies, Anti-Idiotypic Binding Sites BLOOD Blood Volume Cells Centrifugation Chloride, Ammonium Common Cold Cytokeratin DAPI Erythrocytes Goat Leukocyte Count Leukocytes Malignant Neoplasms Methanol Microscopy Monoclonal Antibodies Mus paraform Patients Retention (Psychology) Serum
3
Unilateral Viral Transduction in Mice
For experiments investigating general transduction efficiency three to seven mice were used per serotype and brain region (Figure 1). Animals were deeply anesthetized with a mixture of ketamine and medetomidine (KM; 2.5 mg ketamine-HCl and 0.02 mg medetomidine-HCl/25 g mouse weight) injected intraperitoneally, and positioned in a stereotaxic frame (Kopf Instruments, Tujunga, CA; Stereotaxic System Kopf 1900). A local anaesthetic (lidocaine) was applied subcutaneously before exposure of the skull. Small holes were drilled into the skull and injections were performed unilaterally using a thin glass pipette with 80 nl of virus solution (titer: 9.6 * 1011 viral genomes (VG)/ml in PBS) at a flow rate of 20 nl/min (World Precision Instruments, Sarasota, FL; Nanoliter 2000 Injector). Glass pipettes (World Precision Instruments, Sarasota, FL; Glass Capillaries for Nanoliter 2000; Order# 4878) had been pulled with a long taper and the tip was cut to a diameter of 20-40µm. After the injection, the pipette was left in place for 3 minutes, before being slowly withdrawn. Coordinates for injections were (in mm: caudal, lateral, and ventral to bregma): striatum (0.9, 1.5, 3.2), hippocampus (-1.9, 1.6, 1.6), cortex (-2.9, 4.25, 2.5). After surgery, anesthesia was neutralized with 0.02 ml atipamezole. Mice were monitored daily and intraperitoneal injections of carprofen (0.2 ml of 0.5 mg/ml stock) were applied on the first days after surgery.
For injections of LPS (Escherichia coli 0127:B8, Sigma-Aldrich, Germany; Figure 4A), mice were anesthetized with 1-2 vol% isoflurane in oxygen and two µl of LPS dissolved in saline (5 µg/µl) were infused at a flow rate of 0.2 µl/min into the striatum (coordinates (in mm) relative to bregma: 0.5, 2.0, -3.5). The cannula was left in place for further 5 minutes before being removed.
In the experiments investigating retrograde transport (Figures 5, 6), three mice were unilaterally injected with 250 nl of a 4:1 mixture of rAAV5 solution (titer s.a.) and cholera toxin subunit B-alexa fluor 555 conjugate (Invitrogen, C-22843; 1 mg/ml in PBS) into the hippocampus (same coordinates as above). Surgery, pharmacology, and injection were carried out as above.
When analyzing the time-course of expression (Figure 7 and Figure S4), mice received 80 nl injections into the striatum (titer: 1.01 * 1012 VG/ml; same coordinates as above). One hemisphere was injected with either a (self-complementing) scGFP/scCherry and the other hemisphere was injected with either a (single strand) ssCherry/ssGFP virus solution. Surgery, pharmacology, and injection as above.
Aschauer D.F., Kreuz S, & Rumpel S. (2013). Analysis of Transduction Efficiency, Tropism and Axonal Transport of AAV Serotypes 1, 2, 5, 6, 8 and 9 in the Mouse Brain. PLoS ONE, 8(9), e76310.
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Publication 2013
Alexa Fluor 555 Anesthesia Animals atipamezole Brain Cannula Capillaries carprofen Cholera Cortex, Cerebral Cranium Escherichia coli Injections, Intraperitoneal Isoflurane Ketamine Ketamine Hydrochloride Lidocaine Local Anesthesia Medetomidine Mice, House Operative Surgical Procedures Oxygen Protein Subunits Reading Frames Saline Solution Seahorses Striatum, Corpus Toxins, Chimeric Viral Genome Virus
4
Measuring Protein Synthesis in Cells
For in vitro analysis 103-104 bone marrow or sorted cells were plated in 100 μl of methionine free Dulbecco’s Modified Eagle’s Medium (Sigma) supplemented with 200 μM L-cysteine (Sigma), 50 μM 2-mercaptoethanol (Sigma), 1mM L-glutamine (Gibco) and 0.1% bovine serum albumin (BSA; Sigma). For analysis of HPG and AHA incorporation, cells were pre-cultured for 45 minutes to deplete endogenous methionine. For OP-Puro, the medium was supplemented with 1mM L-methionine (Sigma). HPG (Life Technologies; 1mM final concentration), AHA (Life Technologies; 1mM final concentration) or OP-Puro (Medchem Source; 50 μM final concentration) were added to the culture medium for 1 hour (HPG and OP-Puro) or 2.5 hours (AHA), then cells were removed from wells and washed twice in Ca2+ and Mg2+ free phosphate buffered saline (PBS). Cells were fixed in 0.5ml of 1% paraformaldehyde (Affymetrix) in PBS for 15 minutes on ice. Cells were washed in PBS, then permeabilized in 200 μl PBS supplemented with 3% fetal bovine serum (Sigma) and 0.1% saponin (Sigma) for 5 minutes at room temperature. The azide-alkyne cycloaddition was performed using the Click-iT Cell Reaction Buffer Kit (Life Technologies) and azide conjugated to Alexa Fluor 488 or Alexa Fluor 555 (Life Technologies) at 5μM final concentration. After the 30 minute reaction, the cells were washed twice in PBS supplemented with 3% fetal bovine serum and 0.1% saponin, then resuspended in PBS supplemented with 4’,6-diamidino-2-phenylindole (DAPI; 4 μg/ml final concentration) and analyzed by flow cytometry. To inhibit OP-Puro, HPG or AHA incorporation, cycloheximide (Sigma) was added 30 minutes prior to OP-Puro or HPG at a final concentration of 100 μg/ml. All cultures were incubated at 37°C in 6.5% CO2 and constant humidity.
For in vivo analysis, OP-Puro (50mg/kg body mass; pH 6.4–6.6 in PBS) was injected intraperitoneally. One hour later mice were euthanized, unless indicated otherwise. Bone marrow was harvested, and 3×106 cells were stained with combinations of antibodies against cell surface markers as described below. After washing, the cells were fixed, permeabilized, and the azide-alkyne cycloaddition was performed as described above. “Relative rates of protein synthesis” were calculated by normalizing OP-Puro signals to whole bone marrow after subtracting autofluorescence background. “Mean OP-Puro fluorescence” reflected absolute fluorescence values for each cell population from multiple independent experiments.
To assess the effect of proteasome activity on OP-Puro incorporation mice were administered an intravenous injection of bortezomib (Cell Signaling; 1mg/kg body mass) 1 hour before OP-Puro administration. OP-Puro incorporation was assessed as described above 1 hour later unless indicated otherwise.
Signer R.A., Magee J.A., Salic A, & Morrison S.J. (2014). Haematopoietic stem cells require a highly regulated protein synthesis rate. Nature, 509(7498), 49-54.
Publication 2014
2-Mercaptoethanol alexa fluor 488 Alexa Fluor 555 Alkynes Antibodies Azides Bone Marrow Bortezomib Cardiac Arrest Cells Culture Media Cycloaddition Reaction Cycloheximide Cysteine DAPI Eagle Fetal Bovine Serum Flow Cytometry Fluorescence Glutamine Human Body Humidity Methionine Multicatalytic Endopeptidase Complex Mus O-propargyl-puromycin paraform Phosphates Protein Biosynthesis Saline Solution Saponin Serum Albumin, Bovine
5
Comparative Analysis of Microglial Isolation Methods
A PubMed search was conducted using the terms “microglia AND (rat OR mouse) AND primary AND culture NOT review [publication type]” from 2006 to 2016. This search resulted in 392 articles. One hundred twenty-five articles were excluded (81 did not use microglial culture, 25 microglia used cell lines, 19 were not available for full-text access), resulting in 267 articles that used primary cultures of rodent microglia. This quick survey of the literature suggested that two most commonly used methods to purify microglia involved shaking and mild trypsinization (204 used shaking and 26 of them used mild trypsinization) (Fig. 1). Based on this initial analysis, we compared microglia obtained with shaking versus trypsinization. Primary rat microglia cultures were prepared from cerebral cortices of 1–2-day-old neonatal Sprague-Dawley rats. After removing the meninges, the cortical tissues were digested with 0.25% trypsin-EDTA for 30 min at 37 °C, followed by mechanical triturating in DMEM/F12 with 10% fetal bovine serum. The mixed cortical cells were passed through a 70-μm nylon mesh cell strainer and plated on non-coated plastic dishes or plates in DMEM/F12 with 10% FBS, and the medium was completely replaced every 3–4 days. After achieving confluency at about 14 days in vitro, the microglia were isolated from mixed glial cultures via either mild trypsinization (enzyme, E) or shaking (S). The mild trypsinization was performed according to previously described methods [19 (link), 20 (link)]. Incubation of mixed glial cultures with a trypsin solution (0.25% trypsin-EDTA diluted 1:4 in DMEM/F12) for 15–25 min resulted in the detachment of an intact layer of cells in one piece. Microglial cells remained attached to the bottom of the well. For the shaking method [21 (link), 22 (link)], confluent mixed glial cultures were placed on an orbital shaker at 220 rpm for 1 h. The supernatant containing the detached microglial cells was collected and re-seeded for 1 h to allow microglial attachment. After 1 h, the nonadherent cells were removed. Microglia isolated from both methods were allowed to rest overnight prior to treatments. To compare the yield, we plated the cells from one brain of neonatal pups into one 6-well plate. Yield was calculated as cell numbers per field (six random fields of ×200 magnification per culture, n = 5 cultures). The cells were fixed in 4% paraformaldehyde for 30 min, blocked with 5% normal horse serum for 1 h, and incubated with primary antibody against Iba-1 (1:100) at 4 °C overnight. After washing, the cells were incubated with Alexa Fluor 555-conjugated secondary antibodies (1:200) for 1 h at room temperature. Negative controls were incubated without primary antibodies, and no immunoreactivity was observed in these controls.

The proportion of various methods that are used for primary rodent microglial culture by searching PubMed using the term “microglia AND (rat OR mouse) AND primary AND culture NOT review [publication type]”

Lin L., Desai R., Wang X., Lo E.H, & Xing C. (2017). Characteristics of primary rat microglia isolated from mixed cultures using two different methods. Journal of Neuroinflammation, 14, 101.
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Publication 2017
Alexa Fluor 555 Antibodies Brain Cell Lines Cells Cortex, Cerebral Edetic Acid Enzymes Equus caballus Hyperostosis, Diffuse Idiopathic Skeletal Immunoglobulins Infant, Newborn Kidney Cortex Meninges Microglia Mus Neuroglia Nylons paraform Rats, Sprague-Dawley Rodent Serum Tissues Trypsin

Most recents protocols related to «Alexa Fluor 555»

1
Phalloidin and DAPI Staining

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Publication 2023
Alexa Fluor 555 Cells DAPI Microscopy Phalloidine
2
Immunofluorescent Analysis of HP1γ and γH2AX
The corresponding cells were fixed in 4% formaldehyde for 10 min, then samples were treated in 0.5% (V/V) Triton X-100 for 15 min and blocked with 5% BSA for 30 min at 37 °C. After incubated with anti-HP1γ antibody (1:100) (Cell Signaling Technology, #2619, Danvers, MA, USA) and anti-γH2AX antibody (1:100) (millipore, 05-636, Darmstadt, Germany) overnight at 4 °C, samples were incubated with Alexa Fluor® 555 donkey anti-rabbit IgG (H + L) or Alexa Fluor® 555 donkey anti-rabbit IgG (H + L) (1:2000) for 60 min at room temperature and nucleus counterstaining with DAPI. Imaging was obtained by the Olympus FV1000 IX81-SIM Confocal Microscope (Olympus, Tokyo, Japan).
Li X., Wang S., Xie Y., Jiang H., Guo J., Wang Y., Peng Z., Hu M., Wang M., Wang J., Li Q., Wang Y, & Liu Z. (2023). Deacetylation induced nuclear condensation of HP1γ promotes multiple myeloma drug resistance. Nature Communications, 14, 1290.
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Publication 2023
Alexa Fluor 555 anti-IgG Antibodies, Anti-Idiotypic Cell Nucleus Cells DAPI Equus asinus Formaldehyde Microscopy, Confocal Rabbits Triton X-100
3
Evaluating BM-MSC Polarization and Morphology
For the evaluation of BM-MSC polarization and the cellular morphology, upon TP treatment, the cells were seeded in 8-well chamber slides (SPL Life Sciences, #30108) at a density of 2x104 cells per well and incubated overnight to allow attachment, followed by treatment with 10-8 M TP for 24 hours. The slides were fixed and permeabilized as previously described23 and labeled either with anti-CXCL9 PE (Clone: J1015E10, Biolegend, #357903) and anti-CXCL5 APC (Clone: J111B7, Biolegend, #524105) antibodies or with F-actin probe Phalloidin (Alexa Fluor 555 conjugated, Thermo Fisher Scientific, #A34055) by incubating overnight at 4 °C. Cell nuclei were counterstained with DAPI, and the slides were mounted with FluoroShield medium (Sigma Aldrich, #F6182). Micrographs were taken with a Zeiss LSM 780 confocal microscope.
Aru B., Akdeniz T., Dağdeviren H., Gürel G, & Yanıkkaya Demirel G. (2023). Testosterone Propionate Promotes Proliferation and Viability of Bone Marrow Mesenchymal Stem Cells while Preserving Their Characteristics and Inducing Their Anti-Cancer Efficacy. Balkan Medical Journal, 40(2), 117-123.
Publication 2023
Alexa Fluor 555 Antibodies Cell Nucleus Cells Clone Cells CXCL5 protein, human CXCL9 protein, human DAPI F-Actin Fluoroshield Microscopy, Confocal Phalloidine
4
Evaluation of Neutrophil Activation Markers
Freund’s complete adjuvant (FCA) (F5881), lipopolysaccharide (LPS) (L2880) and phorbol 12-myristate 13-acetate (PMA) (P1585) were obtained from Sigma Chemicals (Louis, MO, USA). Sinomenine (S2359) was purchased from Selleck Chemicals (Shanghai, CHN). Anti-MPO (ab9535), anti-NE (ab21595), anti-Ly6G (ab25377), anti-CitH3 (ab5103), anti-IL-6 (ab208113), anti-LC3B (ab48394), anti-Beclin-1 (ab207612), anti-GAPDH (ab181602), anti-ERK1/2 (ab115799), anti-rabbit IgG secondary (ab6721) and goat anti-rabbit IgG H&L (Alexa Fluor® 555) secondary antibodies (ab150086) were obtained from Abcam (Cambridge, MA, USA). The anti-p65 (3033S), anti-phospho-p65 (Ser536) (6956S), anti-SAPK/JNK (9252S), anti-phospho-SAPK/JNK (Thr183/Tyr185) (9251S), anti-phospho-ERK1/2 (Thr202/Tyr204) (9101S), anti-p38 (9212S) and anti-phospho-p38 (Thr180/Tyr182) (9211S) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). The anti-PAD4 antibodies (17373-1-AP) were purchased from Proteintech Group, Inc. (Wuhan, HB, CHN). Horseradish peroxidase (HRP)-conjugated goat anti-mouse/rabbit IgG polymer kit was purchased from ZS GB-Bio (Beijing, CHN). Percoll™ PLUS (17-5445-01) was purchased from GE Healthcare (Uppsala, Sweden). The Cytometric Beads Array (CBA) kit (560485) was purchased from BD Biosciences (Becton, Dickinson and Company). Tris-buffered saline Tween-20 (TBST) was purchased from Biorigin (Beijing, CHN). Sodium citrate antigen retrieval solution and RIPA buffer were obtained from Solarbio (Beijing, CHN). The enhanced chemiluminescence (ECL) reagent was obtained from Cwbio IT Group (Beijing, CHN). Cell Counting Kit 8 (CCK-8) was purchased from Analysis Quiz (Beijing, CHN).
Jiang H., Lu Q., Xu J., Huo G., Cai Y., Geng S., Xu H., Zhang J., Li H., Yuan K, & Huang G. (2023). Sinomenine ameliorates adjuvant-induced arthritis by inhibiting the autophagy/NETosis/inflammation axis. Scientific Reports, 13, 3933.
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Publication 2023
Alexa Fluor 555 Anti-Antibodies anti-IgG Antibodies Antigens BECN1 protein, human Buffers Chemiluminescence Freund's Adjuvant GAPDH protein, human Goat Horseradish Peroxidase Lipopolysaccharides Mitogen-Activated Protein Kinase 3 Mus Percoll Polymers Rabbits Radioimmunoprecipitation Assay Saline Solution sinomenine Sodium Citrate Tetradecanoylphorbol Acetate Tween 20
5
Whole-mount Immunofluorescent Staining of Ileal Tissue
Whole-mount immunofluorescent staining was performed using a modification of a previously reported method61 (link). The ileal tissue was fixed in 4% paraformaldehyde (Sigma) for 2 h at room temperature. Fixed tissue was permeabilized with 0.5% Triton X-100 (Sigma) overnight at room temperature, and then blocked with 10% goat serum (Sigma) and 0.5% Triton X-100 overnight at 4 °C. For SD and CDAHFD group, antibody reaction was performed with FITC labeled mouse anti-Crp1 antibody (50 μg/mL, clone 77-R63, produced in our laboratory) and Alexa Fluor 647-labeled anti-mouse/human CD324 (E-cadherin) antibody (1:100, clone DECMA-1, BioLegend). For CDAHFD + PBS and CDAHFD + R-Spo1 group, the primary antibody reaction was performed with rabbit anti-Olfactomedin 4 (Olfm4) antibody (1:80, clone D6Y5A, Cell Signaling) for 1 day at 4 °C, and then the secondary antibody reaction was performed with Alexa Fluor 555 conjugated F(ab′)2-goat anti-rabbit IgG (dilution 1:500, Thermo Fisher Scientific) and FITC labeled mouse anti-Crp1 antibody overnight at 4 °C. After washing, nuclei were stained with DAPI (Thermo Fisher Scientific). Samples were immersed in the optical-clearing solution (RapiClear 1.52, Sunjin Lab).
For quantification of Crp1 fluorescence intensity and counting numbers of Paneth cells and stem cells, Z-stack images were obtained using a confocal microscope (A1, Nikon) equipped with CFI Apo LWD 20X WI λS (Nikon). The number of Paneth cells was quantified by counting Crp1 immunostaining positive cells on 3 fields (150 × 150 μm2) per tissue. The number of stem cells was quantified counting Olfm4 positive cells on 3 fields (150 × 150 μm2) per tissue. Crp1 fluorescence intensity per Paneth cell was measured by creating a region of interest using image analysis software, NIS-Elements AR ver. 5.11 (Nikon), on 3 fields (150 × 150 μm2) per tissue, and the mean intensity per field was calculated. For quantification of the number and diameter of Paneth cell granules, Z-stack images were obtained using A1 with CFI Apo TIRF 60X Oil (Nikon). The number and diameter of Paneth cell granules were measured on 3 fields (33 × 33 μm2, 2 Paneth cells/field) per tissue.
Nakamura S., Nakamura K., Yokoi Y., Shimizu Y., Ohira S., Hagiwara M., Song Z., Gan L., Aizawa T., Hashimoto D., Teshima T., Ouellette A.J, & Ayabe T. (2023). Decreased Paneth cell α-defensins promote fibrosis in a choline-deficient L-amino acid-defined high-fat diet-induced mouse model of nonalcoholic steatohepatitis via disrupting intestinal microbiota. Scientific Reports, 13, 3953.
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Publication 2023
Alexa Fluor 555 Alexa Fluor 647 anti-c antibody anti-IgG Antibodies, Anti-Idiotypic Apolipoprotein A-I CDH1 protein, human Cell Nucleus Cells Clone Cells Cytoplasmic Granules DAPI E-Cadherin Fluorescein-5-isothiocyanate Fluorescence Fluorescent Antibody Technique Goat Homo sapiens Ileum Immunoglobulins Microscopy, Confocal Mus olfactomedin Paneth Cells paraform Rabbits Serum Stem, Plant Stem Cells Technique, Dilution Tissues Triton X-100

Top products related to «Alexa Fluor 555»

1
Alexa fluor 555 by Thermo Fisher Scientific
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Alexa Fluor 555 is a fluorescent dye used in various biological applications. It has an excitation maximum at 555 nm and an emission maximum at 565 nm, making it suitable for detection and labeling in a range of assays and imaging techniques.
2
Alexa fluor 488 by Thermo Fisher Scientific
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Alexa Fluor 488 is a fluorescent dye used in various biotechnological applications. It has an excitation maximum at 495 nm and an emission maximum at 519 nm, producing a green fluorescent signal. Alexa Fluor 488 is known for its brightness, photostability, and pH-insensitivity, making it a popular choice for labeling biomolecules in biological research.
3
4 6 diamidino 2 phenylindole (dapi) by Merck Group
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DAPI is a fluorescent dye that binds strongly to adenine-thymine (A-T) rich regions in DNA. It is commonly used as a nuclear counterstain in fluorescence microscopy to visualize and locate cell nuclei.
4
4 6 diamidino 2 phenylindole (dapi) by Thermo Fisher Scientific
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DAPI is a fluorescent dye used in microscopy and flow cytometry to stain cell nuclei. It binds strongly to the minor groove of double-stranded DNA, emitting blue fluorescence when excited by ultraviolet light.
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Triton x 100 by Merck Group
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Triton X-100 is a non-ionic surfactant commonly used in various laboratory applications. It functions as a detergent and solubilizing agent, facilitating the solubilization and extraction of proteins and other biomolecules from biological samples.
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Hoechst 33342 by Thermo Fisher Scientific
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Hoechst 33342 is a fluorescent dye that binds to DNA. It is commonly used in various applications, such as cell staining and flow cytometry, to identify and analyze cell populations.
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Alexa fluor 647 by Thermo Fisher Scientific
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Alexa Fluor 647 is a fluorescent dye used in various life science applications. It has an excitation maximum at 650 nm and an emission maximum at 665 nm. Alexa Fluor 647 can be used for labeling proteins, nucleic acids, and other biomolecules.
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Bovine serum albumin (bsa) by Merck Group
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Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
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Alexa fluor 555 phalloidin by Thermo Fisher Scientific
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Alexa Fluor 555 Phalloidin is a fluorescent conjugate used for the specific and high-affinity labeling of F-actin in fixed and permeabilized cells. It can be used to visualize the distribution of actin filaments in various cell types.
10
Alexa fluor 555 goat anti rabbit igg by Thermo Fisher Scientific
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Alexa Fluor 555 goat anti-rabbit IgG is a secondary antibody conjugated with a fluorescent dye used in immunodetection and microscopy applications. It binds to rabbit primary antibodies and emits fluorescent signals in the orange-red range when excited with appropriate light sources.

More about "Alexa Fluor 555"

Alexa Fluor 555 is a widely used fluorescent dye in a variety of biological applications, such as immunofluorescence, flow cytometry, and microscopy.
This bright, photostable dye emits an orange-red fluorescence and is commonly conjugated to antibodies, proteins, and other biomolecules to enable visualization and detection.
Similar fluorescent dyes like Alexa Fluor 488, DAPI, and Hoechst 33342 are also commonly used in these applications.
Alexa Fluor 647 is another popular far-red fluorescent dye, while Alexa Fluor 555 Phalloidin and Alexa Fluor 555 goat anti-rabbit IgG are specific conjugates of Alexa Fluor 555.
Optimizing Alexa Fluor 555 protocols can be streamlined with AI-driven platforms like PubCompare.ai.
These tools can help researchers discover the most effective methods from scientific literature, preprints, and patents, leveraging the power of AI-driven optimization.
Combining Alexa Fluor 555 with other reagents like Triton X-100 and Bovine serum albumin can also enhance experimental results.
By utilizing the insights and capabilities of these AI-powered tools, scientists can expereince the benefits of increased efficiency and improved outcomes in their Alexa Fluor 555-based research and applications.