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AMD 3100

AMD 3100 is a computer processor developed by Advanced Micro Devices (AMD).
It is a 3rd generation Ryzen desktop CPU that offers excellent performance for a variety of applications, including scientific research.
PubCompare.ai's powerful AI-driven analysis can help optimize your AMD 3100 research by easily locating relevant protocols from literature, preprints, and patents.
This can identify the best products and protocols for reproducibility and accuracy, taking your AMD 3100 research to the next level.
Leveragae PubCompare.ai's advanced features to streamline your AMD 3100 studies and maximize your research efficiency.

Most cited protocols related to «AMD 3100»

Mobilizing Hematopoietic Stem Cells

Cited 20 times

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Publication 2013

AMD 3100 Biological Assay BLOOD Cells Chemokine Clodronate Flow Cytometry GW 3965 Immunofluorescence Liposomes Mice, House Saline Solution Sulfoxide, Dimethyl Tissues

Bioluminescent Assay of CXCL12 Binding

Cited 15 times

Cells were plated in black wall 96 well plates (1 × 10⁴ cells per well) using a Multidrop 384 dispensing system (Labsystems, Waltham, MA, USA), and experiments were performed the subsequent day. Cells were incubated with various dilutions of CXCL12-GL or GL supernatants as described in figure legends. For competition experiments, cells were incubated with CXCL12 (R&D Systems); CXCR4 antagonist AMD3100 (Sigma, St. Louis, MO, USA); or CXCR7 antagonists CCX733 or CCX754 (gifts of ChemoCentryx) added 30 minutes before incubating cells with CXCL12-GL for 2 hours at 37° C. Cells then were washed twice with PBS before measuring cell-associated bioluminescence. 1 µg/ml coelenterazine in PBS was added to intact cells, and bioluminescence was measured immediately after adding substrate as described above.

Luker K.E., Gupta M, & Luker G.D. (2009). Bioluminescent CXCL12 Fusion Protein for Cellular Studies of CXCR4 and CXCR7. BioTechniques, 47(1), 625-632.

Publication 2009

AMD 3100 antagonists Cells coelenterazine CXCL12 protein, human CXCR4 protein, human CXCR7 protein, human Gifts Technique, Dilution

Humanized Mouse Model for HCMV Infection

Cited 7 times

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Publication 2010

AMD 3100 Blood Cells Cells Cone-Rod Dystrophy 2 Granulocyte Colony-Stimulating Factor Hematopoietic System Homo sapiens Human Herpesvirus 5 Infection Institutional Animal Care and Use Committees Mice, Inbred NOD Mus Osmosis Radiotherapy SCID Mice Skin Stem Cells Umbilical Cord Blood

Quantifying Hematopoietic Stem Cell Mobilization

Cited 6 times

Mice were injected with either histamine (5 mg/kg i.v.) or AMD3100 (5 mg/kg subcutaneously) and/or rhVEGF-165 (2 μg/mouse, i.v.) as indicated. Total blood was isolated by perfusion with PBS/20 mM EDTA and processed for cell counts and flow-cytometry analysis to determine the number and frequency of each cell population as described previously (Smith-Berdan et al., 2011 (link)). Reconstitution assays on mobilized blood were performed by transplanting one-half of the blood mouse equivalent into a lethally irradiated host (1,024 rads). Recipient mice were bled at the indicated intervals after transplantation via the tail vein, and PB was analyzed for donor chimerism as described above and previously (Beaudin et al., 2014; Boyer et al., 2011, 2012; Forsberg et al., 2006; Ooi et al., 2009; Smith-Berdan et al., 2011 ).

Smith-Berdan S., Nguyen A., Hong M.A, & Forsberg E.C. (2015). ROBO4-Mediated Vascular Integrity Regulates the Directionality of Hematopoietic Stem Cell Trafficking. Stem Cell Reports, 4(2), 255-268.

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Publication 2015

AMD 3100 Biological Assay BLOOD Cells Chimerism Edetic Acid Flow Cytometry Histamine Mus Perfusion RRAD protein, human Tail Tissue Donors Transplantation Veins

Evaluating Viral Replication and Neutralization

Cited 6 times

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Publication 2012

Acquired Immunodeficiency Syndrome AMD 3100 Antibodies Antibodies, Neutralizing Biological Assay CCR5 protein, human CD4 Positive T Lymphocytes Cells Clone Cells CXCR4 protein, human DNA Replication HEK293 Cells HIV-1 HIV hyperimmune globulin Homo sapiens Infection inhibitors Negroid Races Serum Southern African People Susceptibility, Disease TAK 779 Titrimetry Virus Virus Replication

Most recents protocols related to «AMD 3100»

Immunophenotyping of Omental Immune Cells

Rabbit monoclonal antibodies against ALDH1A2 (E6O6Q; Cell Signaling Technology), Desmin (Y66; Abcam), and CXCL12 (79018; R&D) were used. Antibodies against B220 (RA3-6B2), CD3 (17A2), CD4 (GK1.5), CD8a (53-6.7), CD11b (M1/70), CD16/32 (93), CD19 (6D5), CD23 (B3B4), CD45 (30-F11), CD105 (MJ7/18), F4/80 (BM8), IgM (RMM-1), MAdCAM-1 (MECA-367), PECAM-1 (390), PNAd (MECA-79), PDPN (8.1.1), TIE2 (TEK4), VCAM-1 (429), and Rat IgG2a,κ isotype control (RTK2758) were obtained from BioLegend. Antibodies against CD140a (APA5) and Msln (295D) were obtained from BD Biosciences and MDL, respectively. ATRA was obtained from Sigma-Aldrich.
For DT-mediated cell ablation, mice were i.p. injected with 100 ng of DT (Sigma-Aldrich). For CXCR4 inhibition, 200 μg of AMD3100 (Sigma-Aldrich) was i.p. injected every day for five times and omenta were harvested 24 h after the last injection. For inhibition of retinoic acid signaling, 500 nmol of pan retinoic acid receptor inverse agonist BMS493 (Tocris) was i.p. injected every day for three times and omenta were harvested 24 h after the last injection.

Yoshihara T, & Okabe Y. (2023). Aldh1a2+ fibroblastic reticular cells regulate lymphocyte recruitment in omental milky spots. The Journal of Experimental Medicine, 220(5), e20221813.

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Publication 2023

5'-N-methylcarboxamideadenosine ALDH1A2 protein, human AMD 3100 Antibodies CD31 Antigens Cells CXCL12 protein, human CXCR4 protein, human Desmin IgG2A Immunoglobulin Isotypes ITGAM protein, human MADCAM1 protein, human MECA-79 antigen Monoclonal Antibodies MSLN protein, human Mus Omentum Psychological Inhibition Rabbits Retinoic Acid Receptor Tretinoin Vascular Cell Adhesion Molecule-1

Investigating CXCR4-dependent CRC Cell Migration

CRC cells were seeded in 24-well plates and grown in culture medium. At 90% confluence, the medium was replaced with medium without FBS, containing or not containing (control) PMPs, and the cells were incubated for 4 h at 37 °C in a humidified atmosphere with 5% CO₂. To determine whether migration was CXCR4-dependent, cells were incubated with the CXCR4 antagonist AMD3100 (at a final concentration of 10 μM) or with anti-CXCR4 antibodies (at a final concentration of 100 μg/ml). SDF-1 (at a final concentration of 400 ng/ml) was added after incubation of CRC cells with PMPS to stimulate CXCR4-dependent cell migration. Cell migration was evaluated by measuring the cell-free surface at the beginning of the experiment (immediately after the scratch was made) and every 2 h until a 24-h period (Additional file 2: Supplementary Methods). The migration and invasion properties of tumour cells labelled with CellTracker after incorporation of PMPs were evaluated in uncoated or Matrigel-coated (10 mg/ml, Thermo Fisher Scientific) Boyden chambers, respectively (Additional file 2: Supplementary Methods).

Kassassir H., Papiewska-Pająk I., Kryczka J., Boncela J, & Kowalska M.A. (2023). Platelet-derived microparticles stimulate the invasiveness of colorectal cancer cells via the p38MAPK-MMP-2/MMP-9 axis. Cell Communication and Signaling : CCS, 21, 51.

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Publication 2023

AMD 3100 Anti-Antibodies Atmosphere Cells Chemokine CXCL12 CXCR4 protein, human matrigel Migration, Cell Neoplasm Invasiveness

Chemokine Receptor Signaling Assay

Cells were plated on coverslips coated with poly-L-lysine (Sigma) in a 6-well plate, and transfected with 2.5ug of respective plasmids with lipofectamine. Cells were serum starved overnight; treated with either 2uM GSK-F1 or 4ug/ml AMD-3100 for 2 hours and then ligand-induced with CXCL12 (200mg/ml, Peprotech). After treatment, cells were fixed with 4% PFA with 0.2% glutaraldehyde at room temperature for 15 minutes. After aspiration, cells were further incubated with 50mM NH4Cl in PBS and washed for 10 minutes, 3 times. Then cells were thoroughly washed with water, and mounted on slides using Vectashield with DAPI. Cells were imaged using a Leica DMi3000 B fluorescence microscope.

Govindarajan B., Sbrissa D., Pressprich M., Kim S., Vaishampayan U., Cher M.L, & Chinni S. (2023). Adaptor proteins mediate CXCR4 and PI4KA crosstalk in prostate cancer cells and the significance of PI4KA in bone tumor growth. Research Square.

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Publication Preprint 2023

AMD 3100 Cells Chemokine CXCL12 DAPI Glutaral Ligands Lipofectamine Lysine Microscopy, Fluorescence Plasmids Poly A Serum

Isolation and Purification of Rhesus Macaque HSPCs

Rhesus macaque PBMCs were isolated from PB using Ficoll-Paque PLUS density gradient medium (GE Healthcare) following manufacturer recommendations. G-CSF-mobilized (Amgen) and plerixafor-mobilized (Amgen) rhesus macaque CD34⁺ HSPCs were collected as described previously.²⁹ (link)^,⁶⁰ (link) In short, the animals were treated with a 5-day course of 15 μg/kg G-CSF (Amgen) subcutaneously and a single subcutaneous dose of 1 mg/kg AMD3100 (Sigma-Aldrich) on the morning of the fifth day, 3–4 h before leukapheresis. A small-volume leukapheresis procedure was performed using a CS3000 cell separator (Baxter Fenwal), and CD34⁺ HSPCs were immunoselected using a rhesus macaque CD34⁺ antibody (clone 12.8, Fred Hutchinson Cancer Research Center) and an anti-mouse IgM bead (Miltenyi Biotech). A CD34⁺ antibody (550761, BD Biosciences) was used to determine the purity of the immunoselected population.

Peslak S.A., Demirci S., Chandra V., Ryu B., Bhardwaj S.K., Jiang J., Rupon J.W., Throm R.E., Uchida N., Leonard A., Essawi K., Bonifacino A.C., Krouse A.E., Linde N.S., Donahue R.E., Ferrara F., Wielgosz M., Abdulmalik O., Hamagami N., Germino-Watnick P., Le A., Chu R., Hinds M., Weiss M.J., Tong W., Tisdale J.F, & Blobel G.A. (2023). Forced enhancer-promoter rewiring to alter gene expression in animal models. Molecular Therapy. Nucleic Acids, 31, 452-465.

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Publication 2023

AMD 3100 Animals anti-IgM Cells Clone Cells Ficoll Granulocyte Colony-Stimulating Factor Immunoglobulins Leukapheresis Macaca mulatta Malignant Neoplasms Mice, House plerixafor

Clonogenic Assay for Radiosensitivity

To determine the radiosensitivity of cancer cell clonogens with or without CXCR4 treatment, we seeded appropriate concentrations of single-cell suspensions in T25 flasks with the intent to generate about 50–100 cell colonies/flask. Irradiation of cells (up to 6 flasks simultaneously) was performed 6 h later when cells were attached to the flask bottom; if used, AMD3100 was added to the flasks 1 h prior to RT or 10 min after RT. Colonies of 50 cells or more developed by surviving PCa clonogens (without changing the culture medium) were fixed, stained with crystal violet, and counted 7days later. To evaluate the treatment’s effects on cell growth, we measured the relative intensity of cell staining per flask using ImageJ software.

Gupta N., Ochiai H., Hoshino Y., Klein S., Zustin J., Ramjiawan R.R., Kitahara S., Maimon N., Bazou D., Chiang S., Li S., Schanne D.H., Jain R.K., Munn L.L., Huang P., Kozin S.V, & Duda D.G. (2023). Inhibition of CXCR4 Enhances the Efficacy of Radiotherapy in Metastatic Prostate Cancer Models. Cancers, 15(4), 1021.

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Publication 2023

AMD 3100 Culture Media CXCR4 protein, human Malignant Neoplasms Radiation Tolerance Radiotherapy Violet, Gentian

Top products related to «AMD 3100»

Amd3100 by Merck Group

Sourced in United States, Germany, United Kingdom, France, Italy, Sao Tome and Principe, Morocco, Macao, Japan, Canada

AMD3100 is a small molecule that acts as a CXCR4 antagonist. It is used as a laboratory tool in research applications.

Amd3100 by Selleck Chemicals

Sourced in United States, China

AMD3100 is a small molecule that functions as a CXCR4 antagonist. It is commonly used in research applications to inhibit the CXCR4 receptor.

Amd3100 by Bio-Techne

Sourced in United Kingdom

AMD3100 is a small molecule that functions as a CXCR4 antagonist. It binds to the CXCR4 receptor, inhibiting the binding of CXCL12 to this receptor.

Fetal bovine serum (fbs) by Thermo Fisher Scientific

Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal

Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Amd3100 by Abcam

Sourced in United Kingdom

AMD3100 is a small molecule that functions as a CXCR4 antagonist. It is commonly used in research applications to inhibit CXCR4-mediated cellular processes.

Matrigel by BD

Sourced in United States, United Kingdom, Germany, China, Canada, Japan, Italy, France, Belgium, Australia, Uruguay, Switzerland, Israel, India, Spain, Morocco, Austria, Brazil, Ireland, Netherlands, Montenegro, Poland, Denmark

Matrigel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins. It is widely used as a substrate for the in vitro cultivation of cells, particularly those that require a more physiologically relevant microenvironment for growth and differentiation.

Amd3100 by MedChemExpress

Sourced in United States, China

AMD3100 is a small molecule that functions as a CXCR4 antagonist. It is commonly used in research applications as a tool compound to study the CXCR4 receptor and its biological role.

Cxcl12 by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Germany

CXCL12 is a recombinant protein that belongs to the CXC chemokine family. It functions as a chemoattractant for a variety of cell types, including lymphocytes, monocytes, and stem cells.

Cxcl12 by R&D Systems

Sourced in United States, Germany, United Kingdom

CXCL12 is a recombinant human chemokine. Chemokines are a family of small cytokines or signaling proteins secreted by a variety of cell types. CXCL12 plays a key role in the trafficking and homing of cells expressing the CXCR4 receptor.

G csf by Amgen

Sourced in United States

G-CSF is a laboratory equipment product that functions as a growth factor, specifically stimulating the production and differentiation of granulocytes, a type of white blood cell. It plays a key role in the regulation of the immune system.

What is the AMD 3100 and what are its key features?

The AMD 3100 is a 3rd generation Ryzen desktop CPU developed by Advanced Micro Devices (AMD). It offers excellent performance for a variety of applications, including scientific research. Some key features of the AMD 3100 include high core counts, strong single-threaded performance, and energy-efficient architecture. This makes it a great choice for powering demanding workloads like data analysis, simulations, and scientific computing.

How can PubCompare.ai help with AMD 3100 research?

PubCompare.ai's powerful AI-driven analysis can help optimize your AMD 3100 research by easily locating relevant protocols from literature, preprints, and patents. The platform can identify the best products and protocols for reproducibility and accuracy, taking your AMD 3100 research to the next level. PubCompare.ai allows you to screen protocol literature more efficiently and leverage AI to pinpoint critical insights. This helps researchers identify the most effective protocols related to the AMD 3100 for their specific research goals. The platform's AI-driven analysis can highlight key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy.

What are some common usage challenges with the AMD 3100?

One common challenge with the AMD 3100 is ensuring optimal cooling and power delivery to handle its high core counts and performance demands. Users may need to invest in a quality CPU cooler and power supply to get the most out of the AMD 3100 in their system. Additionally, some software may not be fully optimized for the AMD 3100's architecture, leading to compatibility or performance issues. Leveraging PubCompare.ai can help identify the best protocols and practices to address these challenges and maximize the AMD 3100's capabilities for your research needs.

Are there different variations or types of the AMD 3100?

While the AMD 3100 is a specific model in the Ryzen 3 desktop CPU lineup, there are other Ryzen 3 processors available with similar core counts and performance characteristics. For example, the AMD Ryzen 3 3300X is another 3rd generation Ryzen 3 CPU that shares many similarities with the 3100 but may offer slight variations in clock speeds, cache sizes, and power consumption. When choosing an AMD 3100 for your research, it's important to carefully consider your specific needs and compare the available options to ensure you select the best fit.

More about "AMD 3100"

AMD 3100 is a powerful desktop CPU developed by Advanced Micro Devices (AMD), part of the renowned Ryzen 3rd generation processor lineup.
This high-performance processor offers excellent capabilities for a wide range of applications, including scientific research and computational workloads.
The AMD 3100 is built on the advanced Zen 2 microarchitecture, delivering impressive core counts, robust multi-threading, and exceptional energy efficiency.
This processor is particularly well-suited for tasks that benefit from its impressive single-threaded performance, such as data analysis, simulations, and algorithm development.
For researchers leveraging the AMD 3100 in their studies, PubCompare.ai's cutting-edge AI-driven analysis can be a game-changer.
This powerful platform helps optimize research by identifying the most relevant protocols, products, and best practices from the scientific literature, preprints, and patent data.
By streamlining the research process and ensuring reproducibility and accuracy, PubCompare.ai can take your AMD 3100-based studies to new heights.
In addition to the AMD 3100, researchers may also find value in exploring related technologies and concepts, such as FBS (Fetal Bovine Serum), Matrigel, CXCL12, and G-CSF (Granulocyte-Colony Stimulating Factor).
These components and biomolecules can be crucial in various experimental setups, from cell culture to in vivo studies.
Integrating PubCompare.ai's AI-powered insights into your workflows can help you navigate these related elements more effectively, ensuring your AMD 3100-based research achieves maximum impact and efficiency.