Log In Sign up
The largest database of trusted experimental protocols
> Chemicals & Drugs > Organic Chemical > Ammonium acetate

Ammonium acetate

Ammonium acetate is a chemical compound with the formula CH3COO-NH4+.
It is a colorless, crystalline solid that is soluble in water and widely used in various scientific and industrial applications.
Ammonium acetate serves as a buffer in analytical chemistry, a preservative in food and pharmaceuticals, and a precursor in organic synthesis.
It is also utilized in the preparation of certain fertilizers and as an ingredient in some personal care products.
Understanding the optimal protocols for utilizing ammonium acetate is crucial for enhancing research efficiency, reproducibility, and reliability across diverse fields.

Most cited protocols related to «Ammonium acetate»

1
Comprehensive Antioxidant and Enzyme Inhibitory Assays
Cited 78 times
Antioxidant (DPPH and ABTS radical scavenging, reducing power (CUPRAC and FRAP), phosphomolybdenum, and metal chelating (ferrozine method)) and enzyme inhibitory activities [cholinesterase (ChE) Elmann’s method], tyrosinase (dopachrome method), α-amylase (iodine/potassium iodide method), and α -glucosidase (chromogenic PNPG method)) were determined using the methods previously described by Zengin et al. (2014) (link) and Dezsi et al. (2015) (link).
For the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging assay: Sample solution (1 mg/mL; 1 mL) was added to 4 mL of a 0.004% methanol solution of DPPH. The sample absorbance was read at 517 nm after a 30 min incubation at room temperature in the dark. DPPH radical scavenging activity was expressed as millimoles of trolox equivalents (mg TE/g extract).
For ABTS (2,2′-azino-bis(3-ethylbenzothiazoline) 6-sulfonic acid) radical scavenging assay: Briefly, ABTS+ was produced directly by reacting 7 mM ABTS solution with 2.45 mM potassium persulfate and allowing the mixture to stand for 12–16 in the dark at room temperature. Prior to beginning the assay, ABTS solution was diluted with methanol to an absorbance of 0.700 ± 0.02 at 734 nm. Sample solution (1 mg/mL; 1 mL) was added to ABTS solution (2 mL) and mixed. The sample absorbance was read at 734 nm after a 30 min incubation at room temperature. The ABTS radical scavenging activity was expressed as millimoles of trolox equivalents (mmol TE/g extract) (Mocan et al., 2016a (link)).
For CUPRAC (cupric ion reducing activity) activity assay: Sample solution (1 mg/mL; 0.5 mL) was added to premixed reaction mixture containing CuCl2 (1 mL, 10 mM), neocuproine (1 mL, 7.5 mM) and NH4Ac buffer (1 mL, 1 M, pH 7.0). Similarly, a blank was prepared by adding sample solution (0.5 mL) to premixed reaction mixture (3 mL) without CuCl2. Then, the sample and blank absorbances were read at 450 nm after a 30 min incubation at room temperature. The absorbance of the blank was subtracted from that of the sample. CUPRAC activity was expressed as milligrams of trolox equivalents (mg TE/g extract).
For FRAP (ferric reducing antioxidant power) activity assay: Sample solution (1 mg/mL; 0.1 mL) was added to premixed FRAP reagent (2 mL) containing acetate buffer (0.3 M, pH 3.6), 2,4,6-tris(2-pyridyl)-S-triazine (TPTZ) (10 mM) in 40 mM HCl and ferric chloride (20 mM) in a ratio of 10:1:1 (v/v/v). Then, the sample absorbance was read at 593 nm after a 30 min incubation at room temperature. FRAP activity was expressed as milligrams of trolox equivalents (mg TE/g extract).
For phosphomolybdenum method: Sample solution (1 mg/mL; 0.3 mL) was combined with 3 mL of reagent solution (0.6 M sulfuric acid, 28 mM sodium phosphate and 4 mM ammonium molybdate). The sample absorbance was read at 695 nm after a 90 min incubation at 95°C. The total antioxidant capacity was expressed as millimoles of trolox equivalents (mmol TE/g extract) (Mocan et al., 2016c (link)).
For metal chelating activity assay: Briefly, sample solution (1 mg/mL; 2 mL) was added to FeCl2 solution (0.05 mL, 2 mM). The reaction was initiated by the addition of 5 mM ferrozine (0.2 mL). Similarly, a blank was prepared by adding sample solution (2 mL) to FeCl2 solution (0.05 mL, 2 mM) and water (0.2 mL) without ferrozine. Then, the sample and blank absorbances were read at 562 nm after 10 min incubation at room temperature. The absorbance of the blank was sub-tracted from that of the sample. The metal chelating activity was expressed as milligrams of EDTA (disodium edetate) equivalents (mg EDTAE/g extract).
For ChE inhibitory activity assay: Sample solution (1 mg/mL; 50 μL) was mixed with DTNB (5,5-dithio-bis(2-nitrobenzoic) acid, Sigma, St. Louis, MO, United States) (125 μL) and AChE [acetylcholines-terase (Electric ell AChE, Type-VI-S, EC 3.1.1.7, Sigma)], or BChE [BChE (horse serum BChE, EC 3.1.1.8, Sigma)] solution (25 μL) in Tris–HCl buffer (pH 8.0) in a 96-well microplate and incubated for 15 min at 25°C. The reaction was then initiated with the addition of acetylthiocholine iodide (ATCI, Sigma) or butyrylthiocholine chloride (BTCl, Sigma) (25 μL). Similarly, a blank was prepared by adding sample solution to all reaction reagents without enzyme (AChE or BChE) solution. The sample and blank absorbances were read at 405 nm after 10 min incubation at 25°C. The absorbance of the blank was subtracted from that of the sample and the cholinesterase inhibitory activity was expressed as galanthamine equivalents (mgGALAE/g extract) (Mocan et al., 2016b (link)).
For Tyrosinase inhibitory activity assay: Sample solution (1 mg/mL; 25 μL) was mixed with tyrosinase solution (40 μL, Sigma) and phosphate buffer (100 μL, pH 6.8) in a 96-well microplate and incubated for 15 min at 25°C. The reaction was then initiated with the addition of L-DOPA (40 μL, Sigma). Similarly, a blank was prepared by adding sample solution to all reaction reagents without enzyme (tyrosinase) solution. The sample and blank absorbances were read at 492 nm after a 10 min incubation at 25°C. The absorbance of the blank was subtracted from that of the sample and the tyrosinase inhibitory activity was expressed as kojic acid equivalents (mgKAE/g extract) (Mocan et al., 2017 (link)).
For α-amylase inhibitory activity assay: Sample solution (1 mg/mL; 25 μL) was mixed with α-amylase solution (ex-porcine pancreas, EC 3.2.1.1, Sigma) (50 μL) in phosphate buffer (pH 6.9 with 6 mM sodium chloride) in a 96-well microplate and incubated for 10 min at 37°C. After pre-incubation, the reaction was initiated with the addition of starch solution (50 μL, 0.05%). Similarly, a blank was prepared by adding sample solution to all reaction reagents without enzyme (α-amylase) solution. The reaction mixture was incubated 10 min at 37°C. The reaction was then stopped with the addition of HCl (25 μL, 1 M). This was followed by addition of the iodine-potassium iodide solution (100 μL). The sample and blank absorbances were read at 630 nm. The absorbance of the blank was subtracted from that of the sample and the α-amylase inhibitory activity was expressed as acarbose equivalents (mmol ACE/g extract) (Savran et al., 2016 (link)).
For α-glucosidase inhibitory activity assay: Sample solution (1 mg/mL; 50 μL) was mixed with glutathione (50 μL), α-glucosidase solution (from Saccharomyces cerevisiae, EC 3.2.1.20, Sigma) (50 μL) in phosphate buffer (pH 6.8) and PNPG (4-N-trophenyl-α-D-glucopyranoside, Sigma) (50 μL) in a 96-well microplate and incubated for 15 min at 37°C. Similarly, a blank was prepared by adding sample solution to all reaction reagents without enzyme (α-glucosidase) solution. The reaction was then stopped with the addition of sodium carbonate (50 μL, 0.2 M). The sample and blank absorbances were read at 400 nm. The absorbance of the blank was subtracted from that of the sample and the α-glucosidase inhibitory activity was expressed as acarbose equivalents (mmol ACE/g extract) (Llorent-Martínez et al., 2016 (link)).
All the assays were carried out in triplicate. The results are expressed as mean values and standard deviation (SD). The differences between the different extracts were analyzed using one-way analysis of variance (ANOVA) followed by Tukey’s honestly significant difference post hoc test with α = 0.05. This treatment was carried out using SPSS v. 14.0 program.
Uysal S., Zengin G., Locatelli M., Bahadori M.B., Mocan A., Bellagamba G., De Luca E., Mollica A, & Aktumsek A. (2017). Cytotoxic and Enzyme Inhibitory Potential of Two Potentilla species (P. speciosa L. and P. reptans Willd.) and Their Chemical Composition. Frontiers in Pharmacology, 8, 290.
Full text: Click here
Publication 2017
2
Comprehensive Plasma Metabolite Profiling
Cited 60 times
We profiled amino acids, biogenic amines, and other polar plasma metabolites using liquid chromatography-tandem mass spectrometry (LC-MS). Formic acid, ammonium acetate, LC-MS grade solvents, and valine-d8 were purchased from Sigma-Aldrich. We purchased the remainder of the isotopically-labeled analytical standards from Cambridge Isotope Labs, Inc. We prepared calibration curves for a subset of the profiled analytes by serial dilution in stock pooled plasma using stable isotope-labeled reference compounds (leucine-13C, 15N, isoleucine-13C6, 15N, alanine-13C, glutamic acid-13C5, 15N, taurine-13C2, trimethylamine-N-oxide-d9). We ran samples with isotope standards for calibration curves at the beginning, middle, and end of each analytical queue. We prepared plasma samples for LC-MS analyses via protein precipitation with the addition of nine volumes of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing two additional stable isotope-labeled internal standards for valine-d8 and phenylalanine-d8. The samples were centrifuged (10 min, 10,000 rpm, 4°C) and the supernatants were injected directly. Detailed methods are provided in the Supplementary Methods.
Wang T.J., Larson M.G., Vasan R.S., Cheng S., Rhee E.P., McCabe E., Lewis G.D., Fox C.S., Jacques P.F., Fernandez C., O’Donnell C.J., Carr S.A., Mootha V.K., Florez J.C., Souza A., Melander O., Clish C.B, & Gerszten R.E. (2011). Metabolite Profiles and the Risk of Developing Diabetes. Nature medicine, 17(4), 448-453.
Publication 2011
acetonitrile Alanine Amino Acids ammonium acetate Biogenic Amines formic acid Glutamic Acid Isoleucine Isotopes Leucine Liquid Chromatography Methanol Phenylalanine Plasma Proteins Solvents Tandem Mass Spectrometry Taurine Technique, Dilution trimethyloxamine Valine
3
Mass Spectrometric Lipid Profiling
Cited 56 times
Experimental spectra were obtained on a LTQ linear ion trap mass spectrometer, a hybrid LTQ-FT-ICR mass spectrometer (Thermo Fisher Scientific) and a 6530 QTOF mass spectrometer (Agilent). All lipid standards were obtained from Sigma/Aldrich and Avanti Polar Lipids. The infusion of lipid standards and extracted lipid samples was performed using a chip based nano-electrospray infusion (Advion Nanomate). Plasma lipids were extracted using methyl-tert-butyl ether (MTBE)24 (link). In brief, methanol (225 μL) was added to 30 μL blood plasma and shaken with an additional 750 μL of methyl-tert-butyl ether solvent. Phase separation of this extract was induced by adding 187.5 μL of water, vortexing and centrifuging the mixture at 14,000 g for 2 min. The upper organic phase was collected and dried in a vacuum centrifuge. After adding 10 μL of 100 mM ammonium acetate to 90 μL of the supernatant, lipid extracts were infused into the mass spectrometers using an Advion Nanomate chip-based infusion system (nanoESI). Ion trap mass spectra were collected in low resolution mode (1,500 resolving power) on the linear ion trap. The data collection method performed a full scan and a data dependent MS/MS scan of the most abundant ions. Different CID voltages in the range from 0V to 100V were used for evaluation of spectra. For abundance calculations standard spectra were scanned in low-resolution mode with 15V, 20V, 25V, 35V, 45V and 55V CID voltage to obtain specific MS/MS fragmentations. All spectra were recorded with the Thermo Xcalibur software. An infusion time of 30 seconds was set up in full scan mode with 0V CID with an additional 30 seconds of data dependent MS/MS scans to obtain tandem mass spectra for the largest peaks. For each sample, around 50 MS/MS scans were averaged. NIST SRM 1950 blood plasma samples were infused for around 10 minutes to allow the acquisition of a higher number of MS/MS scans.
The 6530 QTOF mass spectrometer for measurement of reference compounds was operated with the following parameters. An Agilent JetStream electrospray source was used in infusion mode at a flow rate of 0.25 ml/min for acquiring QTOF MS and MS/MS spectra. Data were collected with a 0.25 s scan rate in both profile and centroid modes, and mass calibration was maintained by constant infusion of reference ions at 121.0509 and 922.0098 m/z. MS/MS data was generated utilizing data-dependent MS/MS triggering with dynamic exclusion. Precursor ions, with a minimum 1 k signal intensity were isolated with a 4 m/z isolation width (medium setting), and a variable collision energy was applied based on precursor ion m/z (10 eV + 0.03 eV × ion m/z). Data were exported into the open exchange format mzXML. Samples were measured in negative and positive mode. For lipid profiling with liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-MS/MS) we used settings from an external reference25 (link), except we choose a scan rate of 4-8 spectra per scan event and collision energies ranging from 20-40eV.
Kind T., Liu K.H., Yup Lee D., DeFelice B., Meissen J.K, & Fiehn O. (2013). LipidBlast - in-silico tandem mass spectrometry database for lipid identification. Nature methods, 10(8), 755-758.
Publication 2013
4
Lipid Extraction from Diluted Plasma
Cited 53 times
The lipid extraction (adapted from Matyash et al. 23 (link)) was carried out in high grade polypropylene deep well plates. Fifty microliters of diluted plasma (50×) (equivalent of 1 μL of undiluted plasma) was mixed with 130 μL of ammonium bicarbonate solution and 810 μL of methyl tert-butyl ether/methanol (7:2, v/v) solution was added. Twenty-one microliters of internal standard mixture was pre-mixed with the organic solvents mixture. The internal standard mixture contained: 50 pmol of lysophasphatidylglycerol (LPG) 17:1, 50 pmol of lysophosphatic acid (LPA) 17:0, 500 pmol of phosphatidylcholine (PC) 17:0/17:0, 30 pmol of hexosylceramide (HexCer) 18:1;2/12:0, 50 pmol of phosphatidylserine (PS) 17:0/17:0, 50 pmol of phosphatidylglycerol (PG) 17:0/17:0, 50 pmol of phosphatic acid (PA) 17:0/17:0, 50 pmol of lysophposphatidylinositol (LPI 17:1), 50 pmol of lysophosphatidylserine (LPS) 17:1, 1 nmol cholesterol (Chol) D6, 100 pmol of diacylglycerol (DAG) 17:0/17:0, 50 pmol of triacylglycerol (TAG) 17:0/17:0/17:0, 50 pmol of ceramide (Cer) 18:1;2/17:0, 200 pmol of sphingomyelin (SM) 18:1;2/12:0, 50 pmol of lysophosphatidylcholine (LPC) 12:0, 30 pmol of lysophosphatidylethanolamine (LPE) 17:1, 50 pmol of phosphatidylethanolamine (PE) 17:0/17:0, 100 pmol of cholesterol ester (CE) 20:0, 50 pmol of phosphatidylinositol (PI) 16:0/16:0. The plate was then sealed with a teflon-coated lid, shaken at 4°C for 15 min, and spun down (3000 g, 5 min) to facilitate separation of the liquid phases and clean-up of the upper organic phase. Hundred microliters of the organic phase was transferred to an infusion plate and dried in a speed vacuum concentrator. Dried lipids were re-suspended in 40 μL of 7.5 mM ammonium acetate in chloroform/methanol/propanol (1:2:4, v/v/v) and the wells were sealed with an aluminum foil to avoid evaporation and contamination during infusion. All liquid handling steps were performed using Hamilton STARlet robotic platform with the Anti Droplet Control feature for organic solvents pipetting.
Surma M.A., Herzog R., Vasilj A., Klose C., Christinat N., Morin-Rivron D., Simons K., Masoodi M, & Sampaio J.L. (2015). An automated shotgun lipidomics platform for high throughput, comprehensive, and quantitative analysis of blood plasma intact lipids. European Journal of Lipid Science and Technology, 117(10), 1540-1549.
Full text: Click here
Publication 2015
1-Propanol Acids Aluminum ammonium acetate ammonium bicarbonate Ceramides Chloroform Cholesterol Cholesterol Esters Diacylglycerol Lipids Lysophosphatidylcholines lysophosphatidylethanolamine lysophosphatidylserine Methanol methyl tert-butyl ether Phosphates Phosphatidylcholines phosphatidylethanolamine Phosphatidyl Glycerol Phosphatidylinositols Phosphatidylserines Plasma Polypropylenes Solvents Sphingomyelins Teflon Triglycerides Vacuum
5
Multidimensional Peptide Separation and Analysis
Cited 51 times

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2015
acetonitrile Acids ammonium acetate Buffers Capillaries formic acid High-Performance Liquid Chromatographies Mass Spectrometry Peptides Pressure Proteins Radionuclide Imaging Silicon Dioxide Tandem Mass Spectrometry

Most recents protocols related to «Ammonium acetate»

1
Spectral Library Generation via SCX Fractionation
For spectral library generation, the SCX stage tips were prepared, and samples were processed as previously mentioned [19 (link)]. Briefly, the tips were activated with 50 µl acetonitrile and the enriched peptides were loaded followed by their elution in 6 buffers: SCX buffer 1: 50 mM ammonium acetate, 20% acetonitrile, 0.5% formic acid; SCX buffer 2: 75 mM ammonium acetate, 20% acetonitrile, 0.5% formic acid; SCX buffer 3: 125 mM ammonium acetate, 20% acetonitrile, 0.5% formic acid; SCX buffer 4: 200 mM ammonium acetate, 20% acetonitrile, 0.5% formic acid; SCX buffer 5: 300 mM ammonium acetate, 20% acetonitrile, 0.5% formic acid; SCX buffer 6: 5% ammonium hydroxide, 80% acetonitrile. All the fractions were dried by vacuum centrifugation and suspended into 0.1% formic acid/2% acetonitrile before injection.
Maity S., Huang Y., Kilgore M.D., Thurmon A.N., Vaasjo L.O., Galazo M.J., Xu X., Cao J., Wang X., Ning B., Liu N, & Fan J. (2024). Mapping dynamic molecular changes in hippocampal subregions after traumatic brain injury through spatial proteomics. Clinical Proteomics, 21, 32.
Full text: Click here
Publication 2024
2
Optimized HPLC Mobile Phase Preparation
Ammonium acetate (≥98% purity, for
HPLC, acetic acid ammonium salt) and formic acid (≥98% purity)
were purchased from Acros Organics (Geel, Belgium). Methanol (for
LC) was purchased from Merck (Darmstadt, Germany). Ammonium hydroxide
solution (30–33% in water, w/w) was purchased from Sigma-Aldrich
(St. Louis, MO, USA). Ammonium acetate (10 mM) and formic acid (0.5%,
v/v) were prepared in 25% (v/v) methanol in deionized water. 225 μL
of ammonium hydroxide solution was mixed with 29.8 mL of 25% (v/v)
methanol in deionized water in order to achieve an approximate volumetric
concentration of 0.5% ammonium hydroxide.
Ochirov O, & Urban P.L. (2024). Spontaneous Recycling of Electrosprayed Sample by Retrograde Motion of Microdroplets. Journal of the American Society for Mass Spectrometry, 35(3), 631-635.
Full text: Click here
Publication 2024
3
HILIC-MS Profiling of Metabolites
Not available on PMC !
Positive ion mode (POS) : mobile phase A : 10 mM ammonium acetate, acetonitrile : water 95 : 5, .1% formic acid ; mobile phase B : 10 mM ammonium acetate, acetonitrile : water 50 : 50, 0.1% formic acid ; negative ion mode (NEG) : mobile phase A : 10 mM ammonium acetate, acetonitrile : water 95 : 5 ; adjust the pH to 8.0 with ammonia. Mobile phase B : 10 mM ammonium acetate, acetonitrile : water 50 : 50 ; adjust the pH to 8.0 with ammonia. The samples were separated by Nexera UHPLC LC-30A ultra-high performance liquid phase system with micro-liter flow rate. The chromatographic column was first balanced with 98% mobile phase A, and then the sample was transported by an automatic sampler to the HILIC column (Waters, ACQUITY UPLC BEH Amide 1.7 μm, 2.1 × 100 mm) at a flow rate of 0.3 mL/min.
Zhao S., Xv J., Tang M., & Hou W. (2024). Exploring the Effects of Different Processing Techniques on the Antitussive Expectorant of Platycodon grandiflorus by Metabonomics and Pharmacologic Methods. Natural Product Communications, 19(9).
Publication 2024
4
Purification and Preparation of β-Galactosidase
β-Galactosidase (G3153-5MG) was purchased from Sigma-Aldrich. The lyophilized powder was resuspended in 200 mM ammonium acetate (pH 6.9) to a final concentration of 50 μM. For native ESIBD, it was desalted by passing through two P6 buffer exchange columns (7326222, Bio-Rad), equilibrated with 200 mM ammonium acetate (pH 6.9) and diluted in 200 mM ammonium acetate (pH 6.9) to reach the concentration of 10 μM, and used without further purification.
For the preparation of plunge-frozen cryo-EM samples, it was desalted, purified, and transferred into a buffer (25 mM tris, 50 mM NaCl, 10 mM EDTA, and 2 mM MgCl2) using a Superdex 200 Increase 10/300 column (28990944, Cytiva Life Sciences). Ammonium acetate (7.5 M; A2706-100ML) for native MS and buffer components for the plunge-freezing solution, tris (93362-250G), NaCl (S3014-500G), EDTA (DS-100G), and MgCl2 (63068-250G) were also purchased from Sigma-Aldrich. All concentrations were calculated with respect to the most abundant oligomers.
Esser T.K., Böhning J., Önür A., Chinthapalli D.K., Eriksson L., Grabarics M., Fremdling P., Konijnenberg A., Makarov A., Botman A., Peter C., Benesch J.L., Robinson C.V., Gault J., Baker L., Bharat T.A, & Rauschenbach S. (2024). Cryo-EM of soft-landed β-galactosidase: Gas-phase and native structures are remarkably similar. Science Advances, 10(7), eadl4628.
Full text: Click here
Publication 2024
5
Enrichment of Hydrophobic Peptides
Not available on PMC !

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2024

Top products related to «Ammonium acetate»

1
Ammonium acetate by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, Spain, India, France, China, Australia, Switzerland, Macao, Sao Tome and Principe, Canada, Ireland, Czechia, Belgium, Netherlands, Poland, Japan, Austria, Hungary, Finland, Mexico, Sweden, Romania
Ammonium acetate is a chemical compound with the formula CH3COONH4. It is a colorless, crystalline solid that is soluble in water and alcohol. Ammonium acetate is commonly used in various laboratory applications, such as pH adjustment, buffer preparation, and as a mobile phase component in chromatography.
2
Formic acid by Merck Group
Sourced in Germany, United States, Italy, United Kingdom, France, Spain, China, Poland, India, Switzerland, Sao Tome and Principe, Belgium, Australia, Canada, Ireland, Macao, Hungary, Czechia, Netherlands, Portugal, Brazil, Singapore, Austria, Mexico, Chile, Sweden, Bulgaria, Denmark, Malaysia, Norway, New Zealand, Japan, Romania, Finland, Indonesia
Formic acid is a colorless, pungent-smelling liquid chemical compound. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid is widely used in various industrial and laboratory applications.
3
Acetonitrile by Merck Group
Sourced in Germany, United States, Italy, India, China, United Kingdom, France, Poland, Spain, Switzerland, Australia, Canada, Brazil, Sao Tome and Principe, Ireland, Belgium, Macao, Japan, Singapore, Mexico, Austria, Czechia, Bulgaria, Hungary, Egypt, Denmark, Chile, Malaysia, Israel, Croatia, Portugal, New Zealand, Romania, Norway, Sweden, Indonesia
Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.
4
Methanol by Merck Group
Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan
Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
5
Acetic acid by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, India, China, France, Spain, Switzerland, Poland, Sao Tome and Principe, Australia, Canada, Ireland, Czechia, Brazil, Sweden, Belgium, Japan, Hungary, Mexico, Malaysia, Macao, Portugal, Netherlands, Finland, Romania, Thailand, Argentina, Singapore, Egypt, Austria, New Zealand, Bangladesh
Acetic acid is a colorless, vinegar-like liquid chemical compound. It is a commonly used laboratory reagent with the molecular formula CH3COOH. Acetic acid serves as a solvent, a pH adjuster, and a reactant in various chemical processes.
6
Ammonium acetate by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Belgium, Canada, Australia, China, Germany, France
Ammonium acetate is a chemical compound that serves as a laboratory reagent. It is a crystalline solid that is highly soluble in water and other polar solvents. Ammonium acetate is commonly used in various analytical techniques, such as liquid chromatography and mass spectrometry, to improve the separation and ionization of analytes.
7
Methanol by Thermo Fisher Scientific
Sourced in United States, United Kingdom, China, Germany, Belgium, Canada, France, India, Australia, Portugal, Spain, New Zealand, Ireland, Sweden, Italy, Denmark, Poland, Malaysia, Switzerland, Macao, Sao Tome and Principe, Bulgaria
Methanol is a colorless, volatile, and flammable liquid chemical compound. It is commonly used as a solvent, fuel, and feedstock in various industrial processes.
8
Acetonitrile by Thermo Fisher Scientific
Sourced in United States, United Kingdom, China, Belgium, Germany, Canada, Portugal, France, Australia, Spain, Switzerland, India, Ireland, New Zealand, Sweden, Italy, Japan, Mexico, Denmark
Acetonitrile is a highly polar, aprotic organic solvent commonly used in analytical and synthetic chemistry applications. It has a low boiling point and is miscible with water and many organic solvents. Acetonitrile is a versatile solvent that can be utilized in various laboratory procedures, such as HPLC, GC, and extraction processes.
9
Sodium hydroxide (naoh) by Merck Group
Sourced in Germany, United States, India, United Kingdom, Italy, China, Spain, France, Australia, Canada, Poland, Switzerland, Singapore, Belgium, Sao Tome and Principe, Ireland, Sweden, Brazil, Israel, Mexico, Macao, Chile, Japan, Hungary, Malaysia, Denmark, Portugal, Indonesia, Netherlands, Czechia, Finland, Austria, Romania, Pakistan, Cameroon, Egypt, Greece, Bulgaria, Norway, Colombia, New Zealand, Lithuania
Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.
10
Formic acid by Thermo Fisher Scientific
Sourced in United States, United Kingdom, China, Germany, Belgium, Canada, France, Australia, Spain, New Zealand, Sweden, Japan, India, Macao, Panama, Czechia, Thailand, Ireland, Italy, Switzerland, Portugal, Poland
Formic acid is a clear, colorless liquid chemical compound used in various industrial and laboratory applications. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid has a pungent odor and is highly corrosive. It is commonly used as a preservative, pH adjuster, and analytical reagent in laboratory settings.

More about "Ammonium acetate"

Ammonium acetate (NH4CH3COO) is a versatile chemical compound with a wide range of applications in various fields.
Also known as acetic acid ammonium salt or ammonium ethanoate, this colorless, crystalline solid is highly soluble in water and has a distinctive odor.
In analytical chemistry, ammonium acetate serves as a crucial buffer, helping to maintain a stable pH in diverse applications such as chromatography, electrophoresis, and mass spectrometry.
Its buffer properties make it an indispensable tool for researchers, ensuring accurate and reproducible results.
Beyond its analytical uses, ammonium acetate finds application as a preservative in food and pharmaceutical industries.
Its antimicrobial properties help to extend the shelf life of perishable products, contributing to improved food safety and quality.
In organic synthesis, ammonium acetate is utilized as a precursor, playing a vital role in the preparation of various organic compounds.
Its versatility extends to the production of certain fertilizers, where it can be incorporated as an essential ingredient.
Ammonium acetate is also a common component in personal care products, such as shampoos and cosmetics, where it may act as a pH adjuster or a mild surfactant.
When working with ammonium acetate, it is crucial to understand the optimal protocols and best practices to ensure the highest levels of research efficiency, reproducibility, and reliability.
Factors such as concentration, pH, and compatibility with other chemicals, like formic acid, acetonitrile, methanol, acetic acid, and sodium hydroxide, must be carefully considered.
By leveraging the insights and protocols surrounding ammonium acetate, researchers can enhance their workflows, improve data quality, and drive advancements across a wide range of scientific and industrial domains.