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Amyl acetate

Amyl acetate is a colorless, volatile liquid with a fruity, banana-like odor.
It is commonly used as a flavoring agent in food and beverages, as well as a solvent in various industrial applications.
This versatile chemical compound has a wide range of uses, from food and fragrance industries to the production of plastics and coatings.
Researchers can explore the latest protocols, pre-prints, and patents related to amyl acetate using the powerful AI-driven comparisons provided by PubCompare.ai, optimizing experiments and unlocking new insights into this important chemical.

Most cited protocols related to «Amyl acetate»

Odorant Delivery and Mixture Protocols

All odorants (amyl acetate: Aa, ethyl butyrate: Eb, hexanone: Heb) were from Sigma-Aldrich. As odorant stimuli, we used the following mixtures: amyl acetate/air 60%/40%, ethyl butyrate/air 60%/40%, 3-hexanone 60%/40%, amyl acetate/ethyl butyrate 60%/40% and 40%/60%, 3-hexanone/ethyl butyrate 60%/40% and 40%/60%. Each stimulus was repeated 9 and 5 times for anesthetized and awake mice datasets respectively. To test the impact of the number of repetitions for each stimulus (see below), we acquired another dataset and used 8 different stimuli, each applied individually 20 times (Fig. S1). All are monomolecular odorants evoking different percept, at least in Humans: amyl acetate, methyl benzoate, ethyl butyrate, geraniol, carvone−(+), carvone−(−), octanal, 3-hexanone.
Four milliliters of pure monomolecular odorant were placed in glass vials. Odorants were delivered for 2 seconds through a custom made olfactometer as described previously [22] (link), [57] (link). The odorant onset was set to arrive during an animal's expiration. An air flow passed through the vials containing the odorants and was further diluted 20 times with clean dry air before being sent to the nose. All mixtures were performed by gas mixing, varying the relative flow of independent stream of odorized air. Because odors were delivered ∼1 cm away from the animal's nose, these values overestimate concentrations actually reaching the nasal cavity. The total flow was constant (0.4 l/min). To maintain a stable odor concentration during the entire stimulus application, we ensured that flows were stationary with a 5 s preloading before the odorant was delivered.

Gschwend O., Beroud J, & Carleton A. (2012). Encoding Odorant Identity by Spiking Packets of Rate-Invariant Neurons in Awake Mice. PLoS ONE, 7(1), e30155.

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Publication 2012

3-hexanone amyl acetate caprylic aldehyde carvone ethyl butyrate geraniol Homo sapiens methyl benzoate Mice, House Nasal Cavity Neoplasm Metastasis Nose Odorants Odors

Olfactory Preference Assay in Drosophila Larvae

We use third-instar, feeding-stage larvae from the Canton-S wild-type strain (Michels et al. 2005 (link)), aged 5 days after egg laying. Flies are kept in mass culture and maintained at 25 °C, 60–70% relative humidity and a 14/10 h light:dark cycle. Experiments are performed in red light under a fume hood at 21–26 °C room temperature.
As olfactory stimuli, we use 3-octanol (3OCT, CAS: 589-98-0; purity: 97%, Merck), 1-octanol (1OCT, CAS: 111-87-5; purity: 99%, Sigma-Aldrich), n-amyl acetate (AM, CAS: 628-63-7, purity: 99%, Merck), and linalool (LIN, CAS: 78-70-6; purity: 97%, Merck); in cases when odors are presented in diluted form, paraffin oil (CAS: 8012-95-1; Sigma-Aldrich) (PAR) is used as diluent. Odors are applied by adding 10 μL of odor substance into teflon containers (inner diameter 5 mm) which can be closed by a perforated lid (7 holes, 0.5-mm diameter). As we dilute odors in PAR, we first test whether PAR might be behaviorally active. Therefore, we test innate preference (see next paragraph, Innate preference tests) between a container with 10 μL of PAR versus an empty container (EM); larvae behave indifferently in this situation (supplementary Figure 1A; one-sample sign [OSS] test: P = 0.791, N = 16); we therefore use the EM condition in all cases where a no-odor reference is needed.
Petri dishes (Sarstedt) with 85-mm inner diameter are filled with 1% agarose (electrophoresis grade; Roth), allowed to solidify, covered with their lids, and then left untreated until the following day. As positive reinforcer, we use 2 mol fructose (FRU, purity: 99%, Roth) added to 1 L of 1% agarose 10 min after boiling. Before experiments, we replace the regular lids of the Petri dishes with lids perforated in the center by fifteen 1-mm holes to improve aeration.

Saumweber T., Husse J, & Gerber B. (2011). Innate Attractiveness and Associative Learnability of Odors Can Be Dissociated in Larval Drosophila. Chemical Senses, 36(3), 223-235.

Publication 2011

1-Octanol amyl acetate Diptera Electrophoresis Fructose Humidity Hyperostosis, Diffuse Idiopathic Skeletal Larva Light linalool Octanols Odors paraffin oils Sense of Smell Sepharose Strains Teflon

Odor Discrimination Training and Memory Assessment

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Publication 2010

Olfactory BOLD and CBVw fMRI Protocol

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Publication 2014

amyl acetate Animals Echo-Planar Imaging Fistula fMRI monocrystalline iron oxide nanoparticle Odors Oil, Mineral Olfactory Bulb pyridine Susceptibility, Disease Training Programs

Olfactory Learning in Drosophila

Groups of 50 male flies were collected 0-1 days after eclosion and trained at 3-5 days of age. Flies were trained in vials (2.5 cm diameter and 9.5 cm height) containing 1% agar. Vials contained 64 evenly-spaced perforations and a mesh lid to allow even distribution of ethanol. Vials were placed into a holder in a 30 cm length × 15 cm height × 15 cm width training chamber. The training chamber (Aladin Enterprises, Inc., San Francisco, CA) had three nozzles to allow for air/odorants/ethanol to stream in and one nozzle for waste to stream out.
Humidified air was bubbled through 95% ethanol to vaporize ethanol with combined flow rate of 80 U vaporized ethanol and 70 U humidified air (where 100 U is equal to 1.7 L/min at room temperature). Humidified air was streamed over odors placed in a 2.5 cm diameter and 13 cm height cylinder at a flow rate of 130 U for training and 100 U for tests. Odors used were 3 mL iso-amyl alcohol (1:36 in mineral oil) and a mixture of 2 mL ethyl acetate (1:36 in mineral oil) and 1 mL acetic acid (1:400 in mineral oil). Odors were replaced daily.
Reciprocal training was performed to ensure that inherent preference for either odor did not affect conditioning. Training consisted of 3 repetitions (spaced by 50 min) of 10 min of odor 1, then 10 min odor 2 plus ethanol. A separate group of flies was simultaneously trained with 10 min odor 2, then 10 min odor 1 plus ethanol. Vials of flies from Group 1 and Group 2 were paired according to placement in the training chamber and tested simultaneously.
The testing chamber was a 6 cm cube with a mesh Y-maze in the middle (Aladin Enterprises, Inc., San Francisco, CA). Odors were streamed in through opposite arms of the Y (each 6 cm). Vials of flies were placed at the lower Y arm and flies climbed up the mesh cylinder and chose between opposing arms of the Y to 2.5 cm diameter, 9.5 cm height vials. After 2 min, vials were removed and capped. The number of flies that moved into the odor 1 and odor 2 vials were counted. A preference index for the odor paired with ethanol was calculated as (# flies in paired odor vial - # flies in unpaired odor vial) / total # flies. A performance index for conditioned odor preference or aversion (CPI) was calculated by averaging the preference indexes for reciprocally trained groups of flies.
Memory was tested 30 min or 24 hrs post-training. Immediately after training, yeast was added to the training vials to ensure flies did not become food deprived prior to test. For experiments lasting several days, flies were trained on food containing 10 g yeast, 10 g sugar and 4 g agar boiled in 200 mL water. All training and tests were performed in a dark room under red light. The temperature was controlled with an oil-filled radiator (DeLonghi TRD0715T, Dubuque, IA) and humidity controlled with a warm-mist humidifier (Vicks V745A, Proctor & Gamble, San Ramon, CA).

Kaun K.R., Azanchi R., Maung Z., Hirsh J, & Heberlein U. (2011). A Drosophila model for alcohol reward. Nature neuroscience, 14(5), 612-619.

Publication 2011

Most recents protocols related to «Amyl acetate»

Spontaneous Odor Aversion in Mice

Mice were kept in their home cage, water-deprived, and habituated to receiving two bottles of water for 1-h/day for 3 days. Then, a simultaneous choice was performed between one bottle containing a scented solution, either iso-amyl acetate or benzaldehyde, diluted in water and one bottle with water only in order to evaluate spontaneous odor aversion. Indeed, if some concentrations of iso-amyl acetate (0.05%) and benzaldehyde (0.01%) solutions are well accepted and considered neutral for the mice when presented alone^{31 (link)}, animals prefer to consume water over these scented solutions when presented a choice^{58 (link),59 (link)}, this allows an evaluation of odor detection. Based on this protocol, we performed two sets of experiments using different concentrations of the odorized solution. The “highest concentrations” (iso-amyl acetate—0.05% and benzaldehyde—0.01%, Sigma-Aldrich) represent concentrations known to be perceived and equally consumed by mice and previously used in odor-conditioning protocols^{31 (link),59 (link)}. The “lowest concentrations” consist of the same solutions diluted 100 times (i.e., iso-amyl acetate—0.0005% and benzaldehyde—0.0001%). In order to promote the choice and decrease the random consumption of a solution, the odor used and the position (left or right) of the odorized solution were randomly assigned, and the top of the bottle lids were spaced about 3 cm from each other.

Terral G., Harrell E., Lepousez G., Wards Y., Huang D., Dolique T., Casali G., Nissant A., Lledo P.M., Ferreira G., Marsicano G, & Roux L. (2024). Endogenous cannabinoids in the piriform cortex tune olfactory perception. Nature Communications, 15, 1230.

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Publication 2024

Genomic DNA Extraction from Cultured Cells

Not available on PMC !

Treated cells (3 x 10 7 ) were suspended in 20 mL 10 mM Tris-HCl, pH 7.4, 0.5 % SDS, 1 mM EDTA, 150 mM NaCl with 2 mg proteinase K and heated at 55° C for 2h. The solution was cooled and treated with one volume of phenol/CHCl3/iso-amyl alcohol (25/24/1). Following vigorous shaking the layers were separated by centrifugation. The aqueous layer was extracted an additional time and the DNA precipitated by addition of 2 mL 3 M sodium acetate and 15 mL isopropanol. The DNA was resuspended in 10 mL Tris-HCl, (pH 7.4) 1 mM EDTA and treated with 50 µg RNase A for 1 h at 37°C, followed by 100 µg proteinase K for 1 h at 55°C. The aqueous layer was extracted once with phenol/CHCl3/iso-amyl alcohol followed by a CHCl3 extraction. DNA was precipitated with 1/10 th volume 3 M sodium acetate and 2.5 volumes of ethanol.

Torres M.A., Sun D., & Spratt T.E. (2024). Activity of DNA polymerase κ across the genome in human fibroblasts.

Publication 2024

Preparing Mites for SEM Imaging

Not available on PMC !

The mites were xed in 2.5% glutaraldehyde solution for overnight. Then they were washed in cacodylate buffer and dehydrated in a graded series of alcohol. Then the samples were in ltrated with amyl acetate and dried to critical point and coated with gold and observed for Scanning Electron Microscopy (SEM) using Carl Zeiss Scanning Electron Microscope MODEL: Gemini; Sigma 300 at 500 (Kilo Volt) KV.

Bhowmik B., Dey B., Das S., Barman G.D., Chanda S., & Mondal R. (2024). Morphometric and molecular identification of Unionicola chelata (Acari: Hydrachnida: Unionicolidae) from Bellamya bengalensis, a gastropod mollusc in South 24 Parganas district, West Bengal, India.

Publication 2024

Amino Acid Mixture Preparation for Olfactory Assays

We diluted n-amyl acetate (AM) diluted 1 in 20 in paraffin oil and filled into custom-made Teflon containers (10 µl). The preparation for a 20 amino acid mixture followed the previously reported procedure [4 (link)]. Briefly, 20 amino acids were mixed into 1% agarose at concentrations of 0.5 mM for each amino acid (10 mM in total). For a list of all chemicals, see table 1.

Table 1.

List of all chemicals used in this study.

chemical	CAS number	supplier
n-amyl acetate	628-63-7	Merck (Darmstadt, Germany)
paraffin oil	8042-47-5	AppliChem (Darmstadt, Germany)
agarose	9012-36-6	Roth (Karlsruhe, Germany)
l-alanin	56-41-7	Sigma-Aldrich (Seelze, Germany)
l-arginine	74-79-3	Sigma-Aldrich
l-asparagine	70-47-3	Sigma-Aldrich
l-aspartic acid	56-84-8	Sigma-Aldrich
l-cysteine	52-90-4	Sigma-Aldrich
l-histidine	71-00-1	Sigma-Aldrich
l-isoleucine	73-32-5	Sigma-Aldrich
l-leucine	61-90-5	Sigma-Aldrich
l-lysine	56-87-1	Sigma-Aldrich
l-glutamine	56-85-9	Sigma-Aldrich
l-glutamic acid	56-86-0	Sigma-Aldrich
l-glycine	56-40-6	Sigma-Aldrich
l-methionine	63-68-3	Sigma-Aldrich
l-phenylalanine	63-91-2	Sigma-Aldrich
l-proline	147-85-3	Sigma-Aldrich
l-serine	56-45-1	Sigma-Aldrich
l-threonine	72-19-5	Sigma-Aldrich
l-tryptophan	73-22-3	Sigma-Aldrich
l-tyrosine	60-18-4	Sigma-Aldrich
l-valine	72-18-4	Sigma-Aldrich

Toshima N, & Schleyer M. (2024). IR76b-expressing neurons in Drosophila melanogaster are necessary for associative reward learning of an amino acid mixture. Biology Letters, 20(2), 20230519.

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Publication 2024

Scanning Electron Microscopy of Leaf Samples

Not available on PMC !

Leaf histopathological assessments were performed only on the leaf-inoculated plants. These were performed through images obtained via scanning electron microscopy. Leaf fragments of 1 cm 2 were pre-fixed in Karnovsky solution, post-fixed with osmium tetraoxide, dehydrated in ethyl series, transferred to amyl acetate, and critical point dried in a carbon dioxide dryer. The samples were then coated with gold using an ion jet, and images were obtained using scanning electron microscope (Jeol JSM-IT300LV, JEOL USA, Inc., Peabody, MA, USA).

Marques M.M.d.S., Silva I.d.O., Bessa L.A., & Vitorino L.C. (2024). Opportunistic pathogenicity observed for the endophytic fungus Diaporthe ueckerae on Gossypium hirsutum plants. Journal of Plant Pathology.

Publication 2024

Top products related to «Amyl acetate»

Amyl acetate by Merck Group

Sourced in United States

Amyl acetate is a colorless, volatile liquid chemical compound. It is commonly used as a solvent and flavoring agent in various industrial and laboratory applications.

Model hcp 2 by Hitachi

Sourced in Japan

The Hitachi Model HCP-2 is a laboratory-grade centrifuge designed for general-purpose applications. It features a maximum speed of 15,000 rpm and a maximum RCF of 21,620 xg. The centrifuge can accommodate a variety of sample sizes and tube types, making it a versatile instrument for a range of laboratory workflows.

Ethanol by Merck Group

Sourced in Germany, United States, United Kingdom, Italy, India, France, China, Australia, Spain, Canada, Switzerland, Japan, Brazil, Poland, Sao Tome and Principe, Singapore, Chile, Malaysia, Belgium, Macao, Mexico, Ireland, Sweden, Indonesia, Pakistan, Romania, Czechia, Denmark, Hungary, Egypt, Israel, Portugal, Taiwan, Province of China, Austria, Thailand

Ethanol is a clear, colorless liquid chemical compound commonly used in laboratory settings. It is a key component in various scientific applications, serving as a solvent, disinfectant, and fuel source. Ethanol has a molecular formula of C2H6O and a range of industrial and research uses.

Acetic acid by Merck Group

Sourced in United States, Germany, United Kingdom, Italy, India, China, France, Spain, Switzerland, Poland, Sao Tome and Principe, Australia, Canada, Ireland, Czechia, Brazil, Sweden, Belgium, Japan, Hungary, Mexico, Malaysia, Macao, Portugal, Netherlands, Finland, Romania, Thailand, Argentina, Singapore, Egypt, Austria, New Zealand, Bangladesh

Acetic acid is a colorless, vinegar-like liquid chemical compound. It is a commonly used laboratory reagent with the molecular formula CH3COOH. Acetic acid serves as a solvent, a pH adjuster, and a reactant in various chemical processes.

Sodium hydroxide (naoh) by Merck Group

Sourced in Germany, United States, India, United Kingdom, Italy, China, Spain, France, Australia, Canada, Poland, Switzerland, Singapore, Belgium, Sao Tome and Principe, Ireland, Sweden, Brazil, Israel, Mexico, Macao, Chile, Japan, Hungary, Malaysia, Denmark, Portugal, Indonesia, Netherlands, Czechia, Finland, Austria, Romania, Pakistan, Cameroon, Egypt, Greece, Bulgaria, Norway, Colombia, New Zealand, Lithuania

Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.

Amyl alcohol by Merck Group

Sourced in Germany

Amyl alcohol is a colorless, flammable liquid organic compound with the chemical formula C5H12O. It is commonly used as a solvent and in the production of other chemicals.

N amyl alcohol by Merck Group

Sourced in Germany

N-amyl alcohol, also known as 1-pentanol, is a clear, colorless liquid chemical compound. It has a molecular formula of C5H12O and a molar mass of 88.15 g/mol. N-amyl alcohol is primarily used as a solvent and as an intermediate in the production of other chemicals.

Sodium acetate by Merck Group

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Sodium acetate is a chemical compound with the formula CH3COONa. It is a common salt that is widely used in various laboratory and industrial applications. Sodium acetate functions as a buffer solution, helping to maintain a specific pH level in chemical reactions and processes.

Ethyl acetate by Merck Group

Sourced in Germany, United States, India, Italy, United Kingdom, Poland, France, Spain, Sao Tome and Principe, China, Australia, Switzerland, Macao, Chile, Belgium, Brazil, Ireland, Canada, Portugal, Indonesia, Denmark, Mexico, Japan

Ethyl acetate is a clear, colorless liquid solvent commonly used in laboratory applications. It has a characteristic sweet, fruity odor. Ethyl acetate is known for its ability to dissolve a variety of organic compounds, making it a versatile tool in chemical research and analysis.

Su8010 by Hitachi

Sourced in Japan, Germany, United States, China

The SU8010 is a field emission scanning electron microscope (FE-SEM) manufactured by Hitachi. It is designed for high-resolution imaging of a wide range of sample types. The SU8010 provides stable and reliable performance, with a high-resolution capability and a wide range of analytical functions.

What are some common uses for amyl acetate?

Amyl acetate is a versatile chemical compound with a wide range of applications. It is commonly used as a flavoring agent in food and beverages, providing a distinctive fruity, banana-like aroma. Additionally, amyl acetate serves as a solvent in various industrial processes, such as the production of plastics, coatings, and other materials. This versatile chemical has applications across industries, from food and fragrance to manufacturing and beyond.

How can PubCompare.ai help with my amyl acetate research?

PubCompare.ai is a powerful AI-driven platform that can enhance your research on amyl acetate. The tool allows you to more efficiently screen protocol literature and leverage AI to pinpoint critical insights. PubCompare.ai can help you identify the most effective protocols related to amyl acetate for your specific research goals. The platform's AI-driven analysis can highlight key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy.

Are there different variations or types of amyl acetate?

Yes, there are different variations of amyl acetate. The most common types are isoamyl acetate and n-amyl acetate. Isoamyl acetate is the primary component of many banana-flavored products, while n-amyl acetate has a slightly different odor profile. The specific type of amyl acetate used can depend on the desired application and the intended effect. Researchers should carefully consider the different variations when designing experiments or selecting solvents for their work.

What are some common challenges with using amyl acetate?

One of the main challenges with using amyl acetate is its high volatility. As a colorless, volatile liquid, amyl acetate can evaporate quickly, which can impact its effectiveness in certain applications. Additionally, the strong, fruity odor of amyl acetate may be undesirable in some contexts. Researchers and industires using amyl acetate must carefully consider factors like storage, handling, and containment to ensure optimal performance and saefty. PubCompare.ai can help identify protocols that address these challenges and provide insights into the most effective use of amyl acetate.

How can I find the most accurate and reproducible protocols for working with amyl acetate?

PubCompare.ai is an excellent tool for finding the most accurate and reproducible protocols for working with amyl acetate. The platform's AI-driven comparisons allow you to quickly screen protocol literature, including journal articles, pre-prints, and patents, to identify the most effective methods. By leveraging PubCompare.ai's powerful analysis, you can pinpoint the key differences between protocols and choose the option that best suits your research needs, ensuring high levels of reproducibility and accuracy. This can be espcially helpful when trying to optimize experiments or unlock new insights related to amyl acetate.

More about "Amyl acetate"

Amyl acetate, also known as isoamyl acetate or 3-methylbutyl acetate, is a versatile and widely used chemical compound.
This colorless, volatile liquid has a distinct fruity, banana-like aroma, making it a popular flavoring agent in food, beverages, and fragrances.
In addition to its applications in the food and fragrance industries, amyl acetate serves as a solvent in various industrial processes, including the production of plastics, coatings, and other materials.
Researchers can explore the latest protocols, preprints, and patents related to amyl acetate using the powerful AI-driven comparisons provided by PubCompare.ai, optimizing experiments and unlocking new insights into this important chemical.
Amyl acetate is structurally related to other acetate esters, such as ethyl acetate, and can be synthesized from precursors like amyl alcohol (also known as n-amyl alcohol) and acetic acid, often with the aid of a catalyst like sodium hydroxide.
This versatile compound finds applications in a wide range of industries, from food and fragrance to industrial solvents and coatings.
Researchers can leverage the comprehensive data and intuitive tools offered by PubCompare.ai to explore the latest advancements in amyl acetate research, including cutting-edge protocols, emerging preprints, and innovative patent-protected technologies.
By optimizing experiments and unlocking new insights, scientists can drive forward the understanding and utilization of this important chemical compound.