A minimal functional rat TRPV1 construct was cloned into a modified Bacmam vector (Invitrogen) containing an N-terminal fusion cassette (Kozac-MBP-Tev protease site) for purification on amylose resin. TRPV1 protein was expressed in HEK293S GnTI− cells grown in suspension at 37°C. Cells were harvested 48 hours post transduction for preparation of crude membrane and subsequent protein purification, as described10 (link). Negative stain EM and cryo-EM were carried out following established protocols15 (link),44 . At 3.4Å resolution, the cryo-EM map was of sufficient quality for de novo atomic model building. A poly-alanine model was first built in Coot and amino acid assignment subsequently achieved based mainly on the clearly defined side chains densities of bulky residues such as Phe, Tyr, and Trp, as well as some Arg and Lys residues.
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Amylose
Amylose
Amylose is a type of starch found in plants, consisting of long, linear chains of glucose units.
It is a key component in various food and industrial applications, contributing to texture, viscosity, and other functional properties.
Amylose plays a crucial role in human nutrition, digestion, and metabolism.
Researchers utilize a variety of techniques, such as spectroscopy and chromatography, to analyze and quantify amylose content.
Optimizing amylose reasearch workflows and identifying effective products can unlock new possibilities in food science, biochemistry, and related fields.
Experiance the power of PubCompare.ai today to discover the best protocols and products for your amylose studies.
It is a key component in various food and industrial applications, contributing to texture, viscosity, and other functional properties.
Amylose plays a crucial role in human nutrition, digestion, and metabolism.
Researchers utilize a variety of techniques, such as spectroscopy and chromatography, to analyze and quantify amylose content.
Optimizing amylose reasearch workflows and identifying effective products can unlock new possibilities in food science, biochemistry, and related fields.
Experiance the power of PubCompare.ai today to discover the best protocols and products for your amylose studies.
Most cited protocols related to «Amylose»
Alanine
Amino Acids
Amylose
Cells
Cloning Vectors
Dietary Fiber
Poly A
Proteins
Resins, Plant
Stains
TEV protease
Tissue, Membrane
Amylose
Buffers
Chloroform
Deoxyribonuclease EcoRI
DNA Chips
DNA Library
Endopeptidase K
Ethanol
Genome
Genome, Mitochondrial
Immunocytochemistry
Immunoglobulins
Maltose
Nucleic Acids
Oligonucleotide Primers
Phenol
Proteins
Ribonuclease H
Ribosomes
Radiolabeled β-catenin (5 μl) was phosphorylated using 300 nM His-tagged GSK3β and 100 nM maltose-binding protein (MBP)–axin in 20 μl of XB containing 10 mM ATP, 20 mM MgSO4, and 50 mM NaCl. For the nonphorphorylated control, β-catenin was incubated as above, except that 50 mM LiCl was used instead of NaCl to inhibit GSK3β. The kinase reactions were incubated in a shaker for 30 min at 20°C and then added to 50 μl of MBP–axin, bound to amylose beads (1 mg of protein per milliliter of beads), and brought up to 250 μl with XB containing 50 mM LiCl. After phorsphorylated and nonphosphorylated β-catenin, respectively, bound to axin beads, the beads were washed three times with 500 μl of XB containing 50 mM LiCl. Dissociation of the bound β-catenin was initiated by adding 1 μM unlabeled recombinant β-catenin (His-tagged, from Sf9 cells) and incubated at 20°C in a shaker. At the appropriate times, 5 μl aliquots of beads were quickly removed, filtered through Wizard minicolumns (Promega, Madison, Wisconsin, United States), and washed with ice-cold XB (3 ml). Proteins bound to beads were eluted from the minicolumns with 20 μl of hot sample buffer, followed by SDS-PAGE and autoradiography.
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Amylose
Autoradiography
Axin Protein
Buffers
Cardiac Arrest
Cold Temperature
CTNNB1 protein, human
GSK3B protein, human
Maltose
Maltose-Binding Proteins
Phosphotransferases
Promega
Proteins
SDS-PAGE
Sf9 Cells
Sodium Chloride
Sulfate, Magnesium
Human BRCA2 cDNA was cloned into phCMV1 with two repeats of (MBP) at the N-terminus. 293TD cells were transfected and harvested 31 hours post-transfection. Extracts were batch bound to Amylose resin. The protein was eluted with 10 mM maltose, loaded onto HiTrap Q, and step eluted at 450 mM NaCl.
Amylose
Cells
DNA, Complementary
Gene, BRCA2
Homo sapiens
Maltose
Proteins
Resins, Plant
Sodium Chloride
Transfection
2',5'-oligoadenylate
Amylose
Anions
Baculoviridae
Cells
DNA
Escherichia coli
Gel Chromatography
Heparin
histone H3 trimethyl Lys4
HMGB1 Protein
Insecta
Molar
Molecular Sieve Chromatography
Peptides
RAG-1 Gene
RAG2 protein, human
Most recents protocols related to «Amylose»
Example 183
To a mixture of LHMDS (1 M, 1.0 mL, 1.5 eq) in THF (3 mL) was added compound 183-1a (143.1 mg, 0.81 mmol, 0.13 mL, 1.2 eq) at 0° C. and stirred for 30 min. Then compound 183-1 (250 mg, 0.67 mmol, 1 eq) in THF (3 mL) was added to the mixture and stirred for 2 hr at 25° C. The reaction mixture was quenched with saturated aq.NH4Cl (4 mL), extracted with EA (5 mL*3). The combined organic phase was washed with H2O (5 mL) and brine (5 mL), dried over Na2SO4, filtered and concentrated in vacuum. The crude product was purified by flash silica gel chromatography. Then the product was purified by SFC (column: Phenomenex-Amylose-1 (250 mm*30 mm, 5 um); mobile phase: [0.1% NH3H2O ETOH]; B %: 35%-35%, min). Compound 217 (68.81 mg, 0.17 mmol, 25.9% yield) was obtained. Compound 216 (27.0 mg, 67 umol, 10.0% yield) was obtained. Compound 216 LCMS (ESI): RT=0.858 min, mass calcd. For C23H17F3N2O, 394.13 m/z found 394.9 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 8.39 (d, J=1.5 Hz, 1H), 8.00 (d, J=8.3 Hz, 1H), 7.94-7.86 (m, 1H), 7.78 (d, J=8.5 Hz, 3H), 7.68-7.59 (m, 3H), 7.52 (dd, J=1.3, 7.0 Hz, 1H), 6.65 (td, J=7.7, 11.0 Hz, 1H), 6.43 (br t, J=5.5 Hz, 1H), 5.46 (d, J=10.8 Hz, 1H), 3.75 (q, J=6.4 Hz, 2H), 2.90-2.75 (m, 2H).
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1H NMR
Amylose
brine
Chromatography
compound 183
Ethanol
Gel Chromatography
Lincomycin
naphthalene-2-carboxamide
Silica Gel
Silicon Dioxide
Vacuum
Amylose content and total and reducing sugar were determined for all 20 single-panicle progenies using the standard protocol described by Sadasivam and Manickam (42 ).
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Amylose
Carbohydrates
The gel consistency (GC) is based on the consistency of a cold 4.4% milled rice paste in 0.2 M KOH (44 (link)). The GC was measured by the length of the cold gel in the culture tube held horizontally for 0.5 to 1 h. The GC of rice with less than 24% amylose is usually soft. The test separated high-amylose rice into three categories as follows:
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Amylose
ARID1A protein, human
Cold Temperature
Oryza sativa
Paste
Rice starch (complex carbohydrate) has attracted attention for its use in foods, extruded products, soups, and dressings due to its small size of starch granules, neutral taste, and soft mouth feel. Similarly, the market value of rice is influenced by its cooking qualities (amylose content, gel consistency, and alkali spreading value) apart from differential amounts of phenolics, flavonoids, antioxidants, head rice yield, etc.
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Alkalies
Amylose
Antioxidants
Attention
Carbohydrates
Cytoplasmic Granules
Dressings
Feelings
Flavonoids
Food
Head
Oral Cavity
Oryza sativa
Starch
Taste
MST assay was carried out as previously described (57 (link)). Briefly, recombinant CRP and AceE were purified with amylose resin (New England BioLabs). The purified CRP (10 μM) was incubated with 30 μM labeling dye for 30 min, followed by 16 serial twofold dilutions into buffer containing DNA. After 5 min of incubation, 4 μl of each reaction was enclosed in premium-coated glass capillaries and subjected to MST on a Monolith NT.115 NanoTemper instrument, at 40% MST power and 40% light-emitting diode power. Data were analyzed by MST software (MO.Affinity, Munich, Germany). The purified AceE and N-hydroxysuccinimide (NHS) ester fluorescent dye solutions were mixed at a ratio of 1:1 and incubated in the dark at room temperature for 30 min. Serial dilutions of ampicillin or pyruvate with PBS buffer were mixed with 20 μM NHS fluorescent-labeled AceE and then incubated at room temperature for 5 min. The sample was loaded into MST-glass capillaries,
and a NanoTemper Monolith NT.115T instrument was used for MST analysis. By plotting the concentration of antibiotic or metabolite as the per mil change of normalized fluorescence [∆Fnorm (‰) = (Antibiotic or metabolite fluorescence − Control fluorescence) / Control fluorescence], curve fitting was performed using GraphPad Prism software 8.0, and dissociation constant (Kd) values were determined. Three independent samples were carried out.
and a NanoTemper Monolith NT.115T instrument was used for MST analysis. By plotting the concentration of antibiotic or metabolite as the per mil change of normalized fluorescence [∆Fnorm (‰) = (Antibiotic or metabolite fluorescence − Control fluorescence) / Control fluorescence], curve fitting was performed using GraphPad Prism software 8.0, and dissociation constant (Kd) values were determined. Three independent samples were carried out.
Ampicillin
Amylose
Antibiotics
Biological Assay
Buffers
Capillaries
Esters
Fluorescence
Fluorescent Dyes
Light
N-hydroxysuccinimide
prisma
Pyruvate
Resins, Plant
Technique, Dilution
Top products related to «Amylose»
Sourced in United States, United Kingdom, Germany, Sweden, China, France
Amylose resin is a chromatography resin used for the purification of proteins and enzymes. It functions by selectively binding to proteins with a high affinity for amylose, a component of starch. This resin can be used to isolate and concentrate target proteins from complex mixtures.
Sourced in United States
Amylose beads are a type of affinity chromatography resin used for the purification of proteins. They are composed of cross-linked amylose, a polysaccharide derived from starch. The beads have a high binding capacity for proteins that contain a specific binding motif, such as the maltose-binding protein (MBP) tag. This allows for the selective capture and purification of tagged proteins from complex mixtures.
Sourced in Ireland
The Amylose/Amylopectin Assay Kit is a laboratory tool designed to quantify the levels of amylose and amylopectin in starch samples. It provides a method to determine the ratio of these two polysaccharides, which are the main components of starch.
Sourced in Germany, United States, United Kingdom, Netherlands, China, Switzerland, Italy, Canada, Spain, India
Ni-NTA agarose is a solid-phase affinity chromatography resin designed for the purification of recombinant proteins containing a histidine-tag. It consists of nickel-nitrilotriacetic acid (Ni-NTA) coupled to agarose beads, which selectively bind to the histidine-tagged proteins.
Sourced in United States, United Kingdom, Sweden, Germany, Japan, Australia, China
Glutathione Sepharose 4B is a chromatography resin used for the purification of proteins tagged with glutathione S-transferase (GST). It consists of the glutathione ligand covalently coupled to Sepharose 4B beads. The resin can be used to efficiently capture and purify GST-tagged proteins from complex samples.
Sourced in United States, United Kingdom
PMAL-c2x is a laboratory product offered by New England Biolabs. It is a fusion protein expression system designed for the purification of recombinant proteins. The core function of PMAL-c2x is to facilitate the expression and purification of proteins of interest.
Sourced in United States
The Amylose resin column is a chromatographic separation tool used for the purification of proteins fused with an amylose-binding tag. It allows for the selective capture and recovery of these fusion proteins from complex sample mixtures.
Sourced in Ireland
K-AMYL is a kit that provides a spectrophotometric method for the measurement of total amylase activity in biological samples. The kit includes all the necessary reagents and controls for the assay.
Sourced in United States, China, Germany
The LightShift Chemiluminescent EMSA Kit is a laboratory tool designed to detect and analyze protein-DNA interactions. It uses chemiluminescent detection to visualize and quantify the binding of proteins to specific DNA sequences.
More about "Amylose"
Amylose, a type of starch found in plants, is a key component in various food and industrial applications.
It consists of long, linear chains of glucose units and plays a crucial role in human nutrition, digestion, and metabolism.
Researchers utilize a variety of techniques, such as spectroscopy and chromatography, to analyze and quantify amylose content.
Amylose resin and amylose beads are commonly used for affinity purification and separation of biomolecules, including proteins and enzymes.
The amylose/amylopectin assay kit provides a convenient way to determine the ratio of these two starch components.
Ni-NTA agarose and Glutathione Sepharose 4B are other related products used for protein purification.
The PMAL-c2x expression system, which utilizes an amylose-binding maltose-binding protein (MBP) tag, enables efficient expression and purification of recombinant proteins.
The amylose resin column is a versatile tool for protein purification, while the K-AMYL kit allows for the quantitative determination of amylose content.
Optimizing amylose research workflows and identifying effective products can unlock new possibilities in food science, biochemistry, and related fields.
The LightShift Chemiluminescent EMSA Kit is a useful tool for studying DNA-protein interactions, which may be relevant in amylose-related research.
Experiance the power of PubCompare.ai today to discover the best protocols and products for your amylose studies and expand your knowledge in this fascinating field.
It consists of long, linear chains of glucose units and plays a crucial role in human nutrition, digestion, and metabolism.
Researchers utilize a variety of techniques, such as spectroscopy and chromatography, to analyze and quantify amylose content.
Amylose resin and amylose beads are commonly used for affinity purification and separation of biomolecules, including proteins and enzymes.
The amylose/amylopectin assay kit provides a convenient way to determine the ratio of these two starch components.
Ni-NTA agarose and Glutathione Sepharose 4B are other related products used for protein purification.
The PMAL-c2x expression system, which utilizes an amylose-binding maltose-binding protein (MBP) tag, enables efficient expression and purification of recombinant proteins.
The amylose resin column is a versatile tool for protein purification, while the K-AMYL kit allows for the quantitative determination of amylose content.
Optimizing amylose research workflows and identifying effective products can unlock new possibilities in food science, biochemistry, and related fields.
The LightShift Chemiluminescent EMSA Kit is a useful tool for studying DNA-protein interactions, which may be relevant in amylose-related research.
Experiance the power of PubCompare.ai today to discover the best protocols and products for your amylose studies and expand your knowledge in this fascinating field.